Molecular markers, primers, reagent kits and applications for identifying rubber tree variety 'Yunyan 272'

By designing molecular markers and specific primer pairs on the chromosome of rubber tree CM021232_1, and combining PCR amplification and Sanger sequencing, the problem of unstable rubber tree variety identification results was solved, and the accurate identification of the 'Yunyan 272' variety was achieved, improving the accuracy of identification and ease of operation.

CN121183016BActive Publication Date: 2026-05-26YUNNAN INST OF TROPICAL CROPS
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
YUNNAN INST OF TROPICAL CROPS
Filing Date
2025-10-21
Publication Date
2026-05-26

AI Technical Summary

Technical Problem

Existing rubber tree variety identification methods rely on morphological characteristics, which are easily affected by environmental factors, leading to unstable identification results. In addition, high-throughput sequencing methods are costly and difficult to accurately distinguish the 'Yunyan 272' variety, which can easily cause variety confusion.

Method used

Using molecular markers located at bases 10110474–10110618 on chromosome CM021232_1, specific primer pairs were designed, and accurate identification of the 'Yunyan 272' variety was achieved through PCR amplification and Sanger sequencing. A simple kit is provided for the operation.

Benefits of technology

It has enabled the specific identification and accurate characterization of the 'Yunyan 272' variety, improved the accuracy of identification, optimized variety selection and regional suitability assessment, and promoted the scientific nature of rubber tree planting and breeding.

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Abstract

This invention relates to the field of variety breeding and identification technology in molecular biology, specifically a molecular marker, primers, kit, and application for identifying the rubber tree variety 'Yunyan 272'. The molecular marker is located at bases 10110474–10110618 on chromosome CM021232_1, and its nucleotide sequence is shown in SEQ ID No. 1. This invention provides molecular marker primers and a kit for the rubber tree variety 'Yunyan 272'. This technology can specifically identify and amplify the DNA sequence of the 'Yunyan 272' variety, enabling accurate identification. This helps improve the accuracy of variety identification, optimize variety selection and regional suitability assessment, thereby providing a scientific basis for rubber tree planting and breeding, and promoting the healthy development of the rubber industry. The molecular marker, primer pairs, or kit of this invention are easy to operate and suitable for large-scale application.
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