Multi-element composite collagen composition capable of promoting absorption and preparation method of multi-element composite collagen composition

Abstract

The invention discloses an absorption-promoting multi-element composite collagen composition and a preparation method thereof, and belongs to the field of cosmetic raw materials, the absorption-promoting multi-element composite collagen composition comprises the following components by mass: 5%-15% of multi-element composite collagen, 0.1%-0.5% of sodium hyaluronate, 3%-7% of ethyl oleate, 2%-5% of azone, citric acid for maintaining pH at 5.5-6.5, and the balance of water, the multi-element composite collagen comprises I type collagen, III type collagen and XVII type collagen. The azone mainly destroys ordered arrangement of cuticle lipid, the ethyl oleate can further dissolve lipid, the combination of the azone and the ethyl oleate can more effectively weaken the barrier effect of skin barrier and promote collagen penetration, and the sodium hyaluronate increases the hydration degree of the cuticle and makes the cuticle swell, so that the compactness of the skin is reduced, and the skin care effect is improved. And a more favorable condition is provided for penetration of the collagen. The formula has good safety and stability, and is suitable for various skin types.

Description

Technical Field

[0001] This invention relates to an absorption-enhancing multi-component collagen composition and its preparation method, belonging to the field of cosmetic raw materials. Background Technology

[0002] Collagen, as an important biomolecule, is widely used in cosmetics, medical devices, and tissue engineering. Its main functions in skin care include moisturizing, anti-wrinkle, and damage repair. However, due to its large molecular weight, collagen has difficulty penetrating the stratum corneum directly, resulting in low absorption efficiency of topical collagen and limiting its effectiveness in deeper layers of the skin. The stratum corneum, the outermost layer of the skin, is composed of keratinocytes and intercellular lipids and is the skin's main barrier. Intercellular lipids, mainly composed of ceramides, cholesterol, and fatty acids, form a highly ordered layered structure that prevents the penetration of most substances. Therefore, effectively improving the transdermal absorption efficiency of collagen to reach deeper layers of the skin has become a significant technological challenge. Currently, various topical collagen products exist on the market, but most only remain on the skin surface, failing to exert deep repair and anti-aging effects. Therefore, developing a technology that can effectively promote transdermal collagen absorption is crucial for improving the efficacy of topical collagen products. Existing transdermal absorption technologies, such as iontophoresis and ultrasound-guided transdermal absorption, while improving the absorption efficiency of certain substances, remain limited in their effectiveness for large molecules like collagen. Furthermore, some transdermal absorption enhancers may irritate the skin, restricting their application in cosmetics. Therefore, there is a need to develop a safe, effective, and suitable transdermal absorption enhancement technology for collagen. Summary of the Invention

[0003] To overcome the shortcomings of the prior art, the present invention provides an absorption-promoting multi-component collagen composition and its preparation method, which can safely and effectively promote collagen penetration.

[0004] The technical solution adopted by this invention to solve its technical problem is: In a first aspect, this application provides an absorption-promoting multi-component collagen composition, comprising, by weight: 5%-15% multi-component collagen, 0.1%-0.5% sodium hyaluronate, 3%-7% ethyl oleate, 2%-5% azone, and citric acid to maintain the pH at 5.5-6.5, with the balance being water. The multi-component collagen includes type I collagen, type III collagen, and type XVII collagen.

[0005] The absorption-enhancing multi-component collagen composition provided in this application can significantly improve the transdermal absorption efficiency of collagen, enabling it to better reach the deep layers of the skin, increase the concentration of collagen in skin tissue, and promote the absorption and utilization of collagen.

[0006] Furthermore, the mass ratio of the type I collagen, the type III collagen, and the type XVII collagen is 8-10:1:0.1-0.5.

[0007] Type III collagen molecules are softer and more elastic, playing a lubricating role in this system. An appropriate proportion of Type III collagen, with its flexible chains, interweaves between the rigid fibers of Type I collagen, reducing intermolecular hydrogen bonds and hydrophobic interactions, thereby decreasing the tendency of the complex to aggregate. This directly enhances the transdermal potential. Simultaneously, the charge of Type III collagen forms a dynamic equilibrium with that of Type I collagen under weakly acidic conditions, preventing excessive self-assembly and maintaining a permeable state. A low proportion of Type XVII collagen avoids excessive interference with overall charge or viscosity, but is sufficient to transiently interact with integrins or laminin on the surface of keratinocytes. This interaction may locally perturb the connecting proteins between keratinocytes, temporarily increasing intercellular pathways, and synergistically with the transdermal promotion mechanisms of ethyl oleate and azone to reduce collagen retention on the surface.

[0008] This ratio also benefits product storage stability. This specific ratio keeps the net charge of the multi-component collagen near neutral, preventing excessive repulsion from the negatively charged sodium hyaluronate and reducing electrostatic adsorption to skin lipids (facilitating penetration). The anionic chains of sodium hyaluronate form a controllable hydrogen bond network with the collagen complex in a specific ratio, further stabilizing the dispersion system and preventing precipitation during storage or application.

[0009] Furthermore, the molecular weight of the sodium hyaluronate is 1 million Da to 1.8 million Da.

[0010] When the molecular weight of sodium hyaluronate is below 1 million Da, the chains are too short. Although they are easy to diffuse, their film-forming and hydrating abilities are insufficient, making it unable to effectively soften the stratum corneum. When the molecular weight is above 1.8 million Da, the chains are too long, leading to excessive viscosity and hindering the movement of collagen and transdermal penetration enhancers. Sodium hyaluronate with an appropriate molecular weight can form a dynamic complex with multi-component collagen, reducing the non-specific adsorption of collagen to stratum corneum lipids. This allows ethyl oleate and azone to more effectively guide collagen across the lipid barrier.

[0011] Furthermore, the aforementioned absorption-enhancing multi-component collagen composition also contains 0.05%-0.15% disodium ethylenediaminetetraacetate by weight.

[0012] Disodium EDTA exhibits high chelating efficiency within this pH range (5.5-6.5). Intercellular lipids in the stratum corneum (such as ceramides) often bind to calcium ions to maintain their layered structure. Disodium EDTA enhances the penetration-promoting effects of ethyl oleate and azone by chelating calcium ions, locally weakening the stability of the lipid bilayer. Low dosages of disodium EDTA avoid over-chelation that could damage the skin's natural barrier and cause irritation. Furthermore, disodium EDTA prevents precipitation or discoloration caused by metal ions during storage.

[0013] Furthermore, the aforementioned absorption-promoting multi-component collagen composition also contains 0.1%-0.3% vitamin E and 0.5%-1% phenoxyethanol by weight.

[0014] Ethyl oleate, as a transdermal penetration enhancer, may induce localized oxidative stress (such as lipid peroxidation) when disrupting the lipid barrier of the stratum corneum. Vitamin E, through its phenolic hydroxyl groups, captures free radicals, preventing this oxidative damage, thereby maintaining the integrity of the stratum corneum and reducing skin irritation. Citric acid itself has mild antioxidant properties; the combination of the two provides dual protection against the oxidative degradation of collagen and sodium hyaluronate. The antioxidant properties of vitamin E also help prevent the oxidative inactivation of phenoxyethanol during storage.

[0015] Secondly, this application provides a method for preparing an absorption-enhancing multi-component collagen composition, comprising the following steps: Type I collagen, Type III collagen, and Type XVII collagen are mixed to form a multi-component collagen complex; Heat water to 40℃-50℃, add sodium hyaluronate, dissolve it, then add the multi-component collagen and stir to mix. Continue adding azone and ethyl oleate, stir and mix, then adjust the pH to 5.5-6.5 with citric acid, cool to room temperature, and obtain the absorption-promoting multi-component collagen composition. The absorption-promoting multi-component collagen composition comprises, by weight, 5%-15% of the multi-component collagen, 0.1%-0.5% of the sodium hyaluronate, 3%-7% of the ethyl oleate, and 2%-5% of the azone.

[0016] Adding azone and ethyl oleate first may result in excessively high local concentrations, disrupting the sodium hyaluronate-collagen complex. However, adding them after the collagen is evenly dispersed allows azone and ethyl oleate to gradually embed into the complex's periphery, binding to the nonpolar regions of collagen through hydrophobic interactions to form transdermal precursors and prevent phase separation. Finally, citric acid is used to adjust the pH, minimizing the repulsion between collagen and sodium hyaluronate while maximizing the activity of ethyl oleate and azone.

[0017] Furthermore, the step of adding sodium hyaluronate includes: mixing sodium hyaluronate with glycerin until homogeneous and then adding it to water; the amount of glycerin used is equivalent to 0.8 to 2 times the mass of sodium hyaluronate.

[0018] Glycerol's hydroxyl groups form strong hydrogen bonds with the carboxyl and glycosidic bonds of sodium hyaluronate. During mixing, glycerol molecules temporarily insert into the interchain of sodium hyaluronate, preventing the formation of irreversible gel blocks in water. This effect is optimal at specific dosage ratios (0.8-2 times), providing a smooth dispersion medium for collagen when added to multi-component collagen complexes. Glycerol also optimizes collagen stability; its hydroxyl groups form weak hydrogen bonds with the polar groups of collagen, reducing conformational changes under heating and thus preserving bioactivity. Furthermore, glycerol itself softens the stratum corneum, acting more gently than azone. Glycerol increases skin hydration, while ethyl oleate and azone disrupt lipids; together, these three components create a multi-mechanism transdermal pathway, enhancing the penetration depth of collagen.

[0019] Furthermore, after the step of adding azone and ethyl oleate, ultrasonic treatment is performed with an ultrasonic power of 100W-200W and an ultrasonic time of 10min-20min.

[0020] Ultrasonic treatment reduces the size of the multi-component collagen-sodium hyaluronate complex: ultrasonic shear force breaks down weak aggregates of collagen, forming smaller, more uniform particles. In this treatment, too low a power or too short a time will result in insufficient effect; too high a power or too long a time may lead to collagen denaturation or sodium hyaluronate chain breakage. Azone distributes more evenly into the composition under ultrasound, and its penetration-enhancing effect is amplified due to the reduced particle size.

[0021] Furthermore, after the step of adding azone and ethyl oleate, a high-pressure homogenization process is performed at a homogenization pressure of 50 MPa-80 MPa.

[0022] Ethyl oleate and azone are forcibly emulsified into nanoemulsion droplets under high pressure. These droplets bind with collagen complexes through hydrophobic interactions, forming stable composite microparticles. The homogenized nanoparticles can more easily penetrate the stratum corneum pretreated with ethyl oleate and azone because their reduced size increases their specific surface area, facilitating interaction with skin lipids.

[0023] Furthermore, before the step of adding the multi-component collagen, a vacuum degassing process is performed with a vacuum degree of -0.08 MPa and a degassing time of 30 minutes.

[0024] Vacuum degassing effectively removes dissolved oxygen from water at -0.08 MPa, reducing the risk of oxidation. Degassing also improves the oxidative stability of the composition. Furthermore, it helps reduce foam formation in subsequent steps.

[0025] The beneficial effects of this invention are as follows: This invention uses a multi-component complex collagen as the main active ingredient to replenish the collagen needed by the skin. Azone and ethyl oleate can significantly improve the transdermal absorption efficiency of collagen. Azone can alter the structure of intercellular lipids in the stratum corneum, increasing skin permeability. Ethyl oleate can promote collagen penetration into the deep layers of the skin. Sodium hyaluronate has excellent moisturizing properties, increasing skin hydration and further promoting collagen absorption. Specifically, azone mainly disrupts the orderly arrangement of lipids in the stratum corneum, while ethyl oleate can further dissolve lipids. The combination of the two can more effectively weaken the barrier effect of the skin barrier, promoting collagen penetration. Sodium hyaluronate increases the hydration level of the stratum corneum, causing it to swell, thereby reducing skin density and providing more favorable conditions for collagen penetration. This formula has good safety and stability and is suitable for various skin types. Detailed Implementation

[0026] To make the objectives, technical solutions, and advantages of the embodiments of this disclosure clearer, the technical solutions of the embodiments of this disclosure are described clearly and completely below. Obviously, the described embodiments are only some, not all, of the embodiments of this disclosure. All other embodiments obtained by those skilled in the art based on the described embodiments of this disclosure without creative effort are within the scope of protection of this invention.

[0027] It should be understood that, without conflict, any and all embodiments of the present invention can be combined with technical features of any other embodiment or multiple other embodiments to obtain other embodiments. The present invention includes such combinations to obtain other embodiments.

[0028] In this specification, groups and their substituents may be selected by those skilled in the art to provide stable structural moieties and compounds. When a substituent is described by a conventional chemical formula written from left to right, the substituent also includes chemically equivalent substituents obtained when the structural formula is written from right to left.

[0029] Unless otherwise specified, all technical and scientific terms used herein have the standard meaning in the field to which the claimed subject matter pertains. Where multiple definitions exist for a term, the definition herein shall prevail.

[0030] This application provides an absorption-enhancing multi-component collagen composition, comprising the following components by mass: 5%-15% multi-component collagen, 0.1%-0.5% sodium hyaluronate, 3%-7% ethyl oleate, 2%-5% azone, and citric acid to maintain the pH at 5.5-6.5, with the balance being water. The multi-component collagen includes type I collagen, type III collagen, and type XVII collagen.

[0031] Type I collagen is the most abundant type in the skin, but it is difficult to penetrate the stratum corneum when used alone. Type III collagen, with its more flexible molecular chains, fills the gaps in the network of Type I collagen, reducing its tendency to aggregate and thus lowering its overall hydrodynamic size, facilitating penetration. Type X and VII collagen mimic the skin's natural components, temporarily disrupting intercellular connections locally and creating channels for the penetration of other collagens. This multi-component complex not only avoids the limitations of a single type of collagen but also enhances its adaptability to the skin's environment.

[0032] Sodium hyaluronate not only acts as a moisturizer, but more importantly, it works synergistically with multi-component collagen and transdermal penetration enhancers. Sodium hyaluronate itself is a highly hydrophilic polysaccharide that can form a hydrating layer on the skin surface, softening the stratum corneum. Ethyl oleate can insert into the intercellular lipids of the stratum corneum, increasing lipid fluidity, while azone expands the intercellular space by interacting with cell membrane lipids.

[0033] Ethyl oleate primarily targets lipids in the stratum corneum, increasing permeability by disrupting the ordered structure of lipids, while azone focuses more on altering cell membrane fluidity; their mechanisms of action are complementary. Within the pH range of 5.5-6.5, this not only matches the physiological pH of the skin (reducing irritation) but also optimizes the activities of both ethyl oleate and azone.

[0034] Specifically, the preparation steps are as follows: Heat purified water to 40-50℃, slowly add sodium hyaluronate while stirring at 200-300 rpm, and stir for 1-2 hours.

[0035] Slowly add the multi-component collagen at a stirring speed of 100-200 rpm and stir for 1-2 hours. Control the water temperature to not exceed 50℃ to prevent collagen denaturation.

[0036] Mix azone and ethyl oleate in a specific ratio until homogeneous, then add this mixture to the above-mentioned solution containing multi-component collagen. Continue stirring for 30 minutes. Ultrasonic dispersion can be used, with an ultrasonic power of 100-200W and an ultrasonic time of 10-20 minutes.

[0037] The mixture was cooled to room temperature and filtered using a 0.22 μm filter membrane to remove impurities and microorganisms, thereby improving product quality and safety.

[0038] The filtered solution is filled into sterile containers and sealed for storage.

[0039] Preferably, the absorption-enhancing multi-component collagen composition includes: Multi-component collagen 5-15 parts by weight, azone 2-5 parts by weight, ethyl oleate 3-7 parts by weight, sodium hyaluronate (molecular weight: 1 million-1.8 million Da) 0.1-0.5 parts by weight, vitamin E 0.1-0.3 parts by weight, disodium EDTA 0.05-0.15 parts by weight, phenoxyethanol 0.5-1.0 parts by weight, citric acid to adjust pH to 5.5-6.5, purified water: make up to 100 parts by weight.

[0040] The chain length of sodium hyaluronate within this molecular weight range maintains system homogeneity without excessively increasing diffusion resistance. Vitamin E scavenge free radicals, preventing the oxidation of collagen and ethyl oleate, thus maintaining the product's activity and appearance. Disodium EDTA chelates metal ions, preventing them from catalyzing collagen degradation, thereby extending the product's shelf life. Phenoxyethanol inhibits microbial growth, preventing product spoilage and ensuring product safety. The phenolic hydroxyl groups in Vitamin E can interact with the ester groups in ethyl oleate through hydrogen bonding, forming a stable complex and preventing ethyl oleate oxidation. Compared to the basic formulation, this optimized solution exhibits higher stability, extends product shelf life, and ensures product efficacy and safety. Specifically, it prevents collagen aggregation or precipitation, prevents the separation of azone and ethyl oleate, maintains product homogeneity, slows down sodium hyaluronate degradation, maintains the product's moisturizing effect, prevents microbial contamination, and ensures product safety.

[0041] Accordingly, the preparation steps are as follows: The purified water was heated to 40-50℃ and protected with nitrogen for 30 minutes to remove dissolved oxygen. The purified water was then subjected to vacuum degassing treatment at a vacuum degree of -0.08MPa for 30 minutes to prevent the oxidative degradation of collagen and ethyl oleate.

[0042] Mix sodium hyaluronate with a small amount of glycerin until homogeneous, then add it to purified water and stir at 200-300 rpm for 1-2 hours.

[0043] In the above sodium hyaluronate solution, slowly add the multi-component collagen at a stirring speed of 100-200 rpm and stir for 1-2 hours.

[0044] Dissolve vitamin E in a small amount of ethyl oleate and stir well. Mix azone and vitamin E-containing ethyl oleate evenly, and slowly add this mixture to the collagen-containing solution above, continuing to stir for 30 minutes.

[0045] Dissolve EDTA-2Na in a small amount of purified water and slowly add it to the above mixture. Then add phenoxyethanol and stir until homogeneous.

[0046] Using citric acid to adjust the pH to 5.5-6.5 is beneficial for the stable existence of collagen.

[0047] The mixture was cooled to room temperature and filtered using a 0.22 μm filter membrane to remove impurities and microorganisms, thereby improving product quality and safety.

[0048] The filtered solution is filled into sterile containers and sealed for storage.

[0049] Example 1 The product formula is as follows: Multi-component collagen: 10 parts by weight; Azone: 5 parts by weight; Ethyl oleate: 5 parts by weight; Sodium hyaluronate: 0.3 parts by weight (Maclean, catalog number 768689); Purified water: Add to a total of 100 parts by weight.

[0050] The multi-component collagen includes type I collagen (recombinant collagen, Shanghai Boersen Biotechnology Co., Ltd.), type III collagen (recombinant collagen, Yisheng Biotechnology, catalog number 92616ES80), and type XVII collagen (recombinant collagen, Jiangsu Jinpu Nuoan Biotechnology Co., Ltd.), with a mass ratio of 10:1:0.5.

[0051] The preparation method of the product is as follows: 1. Heat the purified water to 45°C.

[0052] 2. While stirring, add sodium hyaluronate and multi-component collagen in sequence, and stir for 1.5 hours.

[0053] 3. Slowly add azone and ethyl oleate, continue stirring for 30 minutes, and add citric acid to maintain the pH at 5.5.

[0054] 4. Cool to room temperature to obtain a collagen transdermal absorption promoting composition.

[0055] Perform performance testing on the product: Transdermal absorption test: The Franz diffusion cell method was used, with pigskin as a model, to test the transdermal absorption of collagen. The test method followed Appendix 15.5 of the "Cosmetic Safety Technical Specifications" (2015 edition): Franz diffusion cell method. Pigskin was fixed in the center of the diffusion cell, and the prepared sample was applied to the surface of the pigskin. Physiological saline was added to the receiving cell below, and the temperature was maintained at 37℃. Samples were taken every 2 hours to determine the collagen content in the receiving cell. Quantitative analysis of collagen was performed using the BCA protein quantification method.

[0056] Moisturizing performance test: Skin moisture content was tested using a Corneometer CM825. The test method followed Appendix 15.1, Method for Determination of Skin Moisture Content, in the "Cosmetic Safety Technical Specifications" (2015 edition). The product was applied to the inner forearm of volunteers, and skin moisture content was tested after 2 hours and compared with a blank control group.

[0057] Stability test: The product was placed in a 40℃ constant temperature chamber for accelerated aging for 30 days, and the changes in product appearance, pH value, viscosity and other indicators were observed.

[0058] Performance data results: Transdermal absorption: The transdermal absorption over 24 hours was 25.6 ± 3.2 μg / cm³. 2 .

[0059] Moisturizing performance: After 2 hours of use, the skin moisture content increased by 35.2±4.5% compared with the blank control group.

[0060] Stability: After accelerated aging at 40℃ for 30 days, the product appearance showed no significant change, the pH value changed by less than 0.5, and the viscosity changed by less than 10%.

[0061] Example 2 The product formula is as follows: Multi-component collagen: 5 parts by weight; Azone: 5 parts by weight; Ethyl oleate: 3 parts by weight; Sodium hyaluronate: 0.1 parts by weight (Maclean's, 768689). Purified water: Add to a total of 100 parts by weight.

[0062] The multi-component collagen includes type I collagen (recombinant collagen, Shanghai Boersen Biotechnology Co., Ltd.), type III collagen (recombinant collagen, Yisheng Biotechnology, catalog number 92616ES80), and type XVII collagen (recombinant collagen, Jiangsu Jinpu Nuoan Biotechnology Co., Ltd.), with a mass ratio of 9:1:0.4.

[0063] The preparation method of the product is as follows: 1. Heat the purified water to 40°C.

[0064] 2. While stirring, add sodium hyaluronate and multi-component collagen in sequence, and stir for 2 hours.

[0065] 3. Slowly add azone and ethyl oleate, continue stirring for 30 minutes, and add citric acid to maintain the pH at 6.3.

[0066] 4. Cool to room temperature to obtain a collagen transdermal absorption promoting composition.

[0067] Performance testing was conducted on the product, and the performance data results are as follows: Transdermal absorption: The transdermal absorption over 24 hours was 27.8 ± 3.0 μg / cm³. 2 .

[0068] Moisturizing performance: After 2 hours of use, the skin moisture content increased by 30.5±3.8% compared with the blank control group.

[0069] Stability: After accelerated aging at 40℃ for 30 days, the product appearance showed no significant change, the pH value changed by less than 0.5, and the viscosity changed by less than 10%.

[0070] Example 3 The product formula is as follows: Multi-component collagen: 10 parts by weight; Azone: 5 parts by weight; Ethyl oleate: 5 parts by weight; Sodium hyaluronate: 0.3 parts by weight (Maclean's, 768689). Vitamin E: 0.2 parts by weight; Disodium ethylenediaminetetraacetate: 0.1 parts by weight; Phenoxyethanol: 0.7 parts by weight; Citric acid: Appropriate amount, adjust the pH value to 5.8; Purified water: Add to a total of 100 parts by weight.

[0071] The multi-component collagen includes type I collagen (recombinant collagen, Shanghai Boersen Biotechnology Co., Ltd.), type III collagen (recombinant collagen, Yisheng Biotechnology, catalog number 92616ES80), and type XVII collagen (recombinant collagen, Jiangsu Jinpu Nuoan Biotechnology Co., Ltd.), with a mass ratio of 8:1:0.1.

[0072] The preparation method of the product is as follows: 1. Heat the purified water to 45°C and purge with nitrogen for 30 minutes.

[0073] 2. While stirring, add sodium hyaluronate and multi-component collagen in sequence, and stir for 1.5 hours.

[0074] 3. Dissolve vitamin E in ethyl oleate and stir well.

[0075] 4. Slowly add azone and the vitamin E-ethyl oleate mixture from step 3, and continue stirring for 30 minutes.

[0076] 5. Dissolve EDTA-2Na in a small amount of purified water and slowly add it to the above mixture, stirring until homogeneous.

[0077] 6. Add phenoxyethanol to the above mixture and stir until homogeneous.

[0078] 7. Adjust the pH to 5.8 using citric acid.

[0079] 8. Cool to room temperature and filter using a 0.22μm filter membrane.

[0080] Performance testing was conducted on the product, and the performance data results are as follows: Transdermal absorption: The transdermal absorption over 24 hours was 29.1 ± 3.5 μg / cm³. 2 .

[0081] Moisturizing performance: After 2 hours of use, the skin moisture content increased by 38.5±4.2% compared with the blank control group.

[0082] Stability: After accelerated aging at 40℃ for 30 days, the product appearance showed no significant change, the pH value changed by less than 0.3, and the viscosity changed by less than 5%.

[0083] Example 4 The product formula is as follows: Multi-component collagen: 15 parts by weight; Azone: 2 parts by weight; Ethyl oleate: 7 parts by weight; Sodium hyaluronate: 0.5 parts by weight (Maclean's, 768689). Vitamin E: 0.3 parts by weight; Disodium ethylenediaminetetraacetate: 0.15 parts by weight; Phenoxyethanol: 1.0 part by weight; Citric acid: Appropriate amount, adjust the pH value to 6.2; Purified water: Add to a total of 100 parts by weight.

[0084] The multi-component collagen includes type I collagen (recombinant collagen, Shanghai Bosheng Biotechnology Co., Ltd.), type III collagen (recombinant collagen, Yisheng Biotechnology, catalog number 92616ES80), and type XVII collagen (recombinant collagen, Jiangsu Jinpu Nuoan Biotechnology Co., Ltd.), with a mass ratio of 8:1:0.2.

[0085] The preparation method of the product is as follows: 1. Heat the purified water to 45°C and purge with nitrogen for 30 minutes.

[0086] 2. While stirring, add sodium hyaluronate and multi-component collagen in sequence, and stir for 1.5 hours.

[0087] 3. Dissolve vitamin E in ethyl oleate and stir well.

[0088] 4. Slowly add azone and the vitamin E-ethyl oleate mixture from step 3, and continue stirring for 30 minutes.

[0089] 5. Dissolve EDTA-2Na in a small amount of purified water and slowly add it to the above mixture, stirring until homogeneous.

[0090] 6. Add phenoxyethanol to the above mixture and stir until homogeneous.

[0091] 7. Adjust the pH to 6.2 using citric acid.

[0092] 8. Cool to room temperature and filter using a 0.22μm filter membrane.

[0093] Performance testing was conducted on the product, and the performance data results are as follows: Transdermal absorption: 30.2 ± 3.3 μg / cm³ over 24 hours. 2 .

[0094] Moisturizing performance: After 2 hours of use, the skin moisture content increased by 40.1±4.8% compared with the blank control group.

[0095] Stability: After accelerated aging at 40℃ for 30 days, the product appearance showed no significant change, the pH value changed by less than 0.3, and the viscosity changed by less than 5%.

[0096] As can be seen from the above embodiments, the formulation of this application has good transdermal absorption and moisturizing properties. After adjusting the formulation in Examples 3 and 4, the stability of the product was significantly improved, and the transdermal absorption and moisturizing properties were also enhanced.

[0097] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "illustrative embodiment," "example," "specific example," or "some examples," etc., indicate that a specific feature, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0098] The above description represents the preferred embodiments of the present invention. It should be noted that those skilled in the art can make various improvements and modifications without departing from the principles of the present invention, and these improvements and modifications are also considered to be within the scope of protection of the present invention.

Claims

1. A multi-component collagen composition that promotes absorption, characterized in that, By weight, it comprises the following components: 5%-15% multi-component collagen, 0.1%-0.5% sodium hyaluronate, 3%-7% ethyl oleate, 2%-5% azone, and citric acid to maintain the pH at 5.5-6.5, with the balance being water. The multi-component collagen includes type I collagen, type III collagen, and type XVII collagen.

2. The absorption-promoting multi-component collagen composition according to claim 1, characterized in that, The mass ratio of the type I collagen, the type III collagen, and the type XVII collagen is 8-10:1:0.1-0.

5.

3. The absorption-promoting multi-component collagen composition according to claim 1, characterized in that, The molecular weight of the sodium hyaluronate is 1 million Da to 1.8 million Da.

4. The absorption-promoting multi-component collagen composition according to claim 1, characterized in that, It also contains 0.05%-0.15% disodium ethylenediaminetetraacetate by weight.

5. The absorption-promoting multi-component collagen composition according to claim 1, characterized in that, By weight, it also contains 0.1%-0.3% vitamin E and 0.5%-1% phenoxyethanol.

6. A method for preparing an absorption-enhancing multi-component collagen composition, characterized in that, Includes the following steps: Type I collagen, Type III collagen, and Type XVII collagen are mixed to form a multi-component collagen complex; Heat water to 40℃-50℃, add sodium hyaluronate, dissolve it, then add the multi-component collagen and stir to mix. Continue adding azone and ethyl oleate, stir and mix, then adjust the pH to 5.5-6.5 with citric acid, cool to room temperature, and obtain the absorption-promoting multi-component collagen composition. The absorption-promoting multi-component collagen composition comprises, by weight, 5%-15% of the multi-component collagen, 0.1%-0.5% of the sodium hyaluronate, 3%-7% of the ethyl oleate, and 2%-5% of the azone.

7. The method for preparing the absorption-enhancing multi-component collagen composition according to claim 6, characterized in that, The step of adding sodium hyaluronate includes: mixing sodium hyaluronate with glycerin until homogeneous and then adding it to water; the amount of glycerin used is equivalent to 0.8 to 2 times the mass of sodium hyaluronate.

8. The method for preparing the absorption-enhancing multi-component collagen composition according to claim 6, characterized in that, After the step of adding azone and ethyl oleate, ultrasonic treatment is performed with an ultrasonic power of 100W-200W and an ultrasonic time of 10min-20min.

9. The method for preparing the absorption-enhancing multi-component collagen composition according to claim 6, characterized in that, After the steps of adding azone and ethyl oleate, a high-pressure homogenization process is performed at a pressure of 50 MPa-80 MPa.

10. The method for preparing the absorption-enhancing multi-component collagen composition according to claim 6, characterized in that, Before adding the multi-component collagen, a vacuum degassing process is performed with a vacuum degree of -0.08 MPa and a degassing time of 30 minutes.