Traditional Chinese medicine composition for treating bovine parainfluenza virus type 3 infection as well as preparation method and application thereof

Abstract

The invention discloses a traditional Chinese medicine composition for treating bovine parainfluenza virus type 3 infection as well as a preparation method and application thereof, and relates to the technical field of traditional Chinese medicine compositions. The traditional Chinese medicine composition comprises the following components in parts by weight: 5-7 parts of scutellaria baicalensis, 4-6 parts of astragalus membranaceus, 4-6 parts of myrobalan, 2-3 parts of honeysuckle, 2-3 parts of fructus forsythiae, 2-3 parts of cyrtomium fortunei, 1-2 parts of folium isatidis and 1-2 parts of liquorice. The invention has a direct inhibition effect on the replication of the BPIV3 virus, has a good clinical application effect, and is suitable for clinical popularization and use.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of traditional Chinese medicine compositions, and more particularly to a traditional Chinese medicine composition for treating bovine parainfluenza virus 3 infection, and a preparation method and application thereof. BACKGROUND

[0002] Bovine parainfluenza virus 3 (BPIV3) can cause "transport fever" in cattle and is one of the main pathogens of bovine respiratory disease complex. BPIV3 infection causes serious harm to the beef cattle industry. Sick cattle initially show symptoms such as elevated body temperature, depression, and reduced appetite, and then show respiratory symptoms such as coughing, runny nose, and rapid breathing. In severe cases, sick cattle can develop pneumonia due to secondary bacterial infection, leading to respiratory distress and even death. BPIV3 is particularly harmful in the rearing of dairy beef calves, and according to statistics, the mortality rate of BPIV3-infected calves can be as high as 10%-15%, and sick calves also experience growth retardation, reduced feed conversion, and other issues, resulting in significant economic losses.

[0003] BPIV3 infection is very common and widely distributed globally, especially in areas with large-scale cattle farming industries, where the prevalence rate is relatively high. Serological surveys in Europe and the United States have shown that the antibody positive rate in calf populations is generally over 60%, and the positive rate can soar to over 80% in winter.

[0004] BPIV3 belongs to the Paramyxoviridae family and is a single-stranded negative-sense RNA virus. Currently, the main methods for preventing and controlling BPIV3 are vaccination and drug treatment. Vaccines include inactivated vaccines and attenuated vaccines, but due to the antigenic variability of the virus, the immune effect of the vaccine is unstable, and the protection rate of some vaccines can only be maintained at 60%-70%. In terms of drug treatment, antibiotics are commonly used to control secondary bacterial infections, but antiviral drugs have limited effectiveness, and long-term use of antibiotics can easily lead to drug resistance, further increasing the difficulty of prevention and control.

[0005] Therefore, it is an urgent problem for those skilled in the art to provide a BPIV3 prevention and control traditional Chinese medicine composition that directly inhibits the replication of BPIV3 virus, has good clinical application effects, and is suitable for clinical promotion and use. SUMMARY

[0006] Therefore, the present application provides a traditional Chinese medicine composition for treating bovine parainfluenza virus 3 infection, and a preparation method and application thereof.

[0007] To achieve the above-mentioned purpose, the present application adopts the following technical solutions:

[0008] A traditional Chinese medicine composition for treating parainfluenza virus type 3 infection, the traditional Chinese medicine composition comprises the following components in parts by weight:

[0009] Scutellaria 5-7 parts, Astragalus 4-6 parts, Terminalia chebula 4-6 parts, honeysuckle 2-3 parts, forsythia 2-3 parts, Piper 2-3 parts, Isatis indigotica 1-2 parts, licorice 1-2 parts.

[0010] Further, the pharmaceutical dosage form of the traditional Chinese medicine composition is powder or oral liquid.

[0011] A preparation method of a traditional Chinese medicine composition for treating parainfluenza virus type 3 infection, the preparation of the oral liquid comprises the following steps:

[0012] (1) Preparation of medicinal materials:

[0013] The medicinal materials are weighed according to the proportion, impurities are removed, and the medicinal materials are crushed into coarse powder and soaked in water for 2 hours;

[0014] (2) Decoction:

[0015] Mix the medicinal materials and water according to a mass ratio of 1:5, boil and decoct at 100℃ for 30 minutes; pour out the medicinal liquid after decoction, filter with gauze; add water to the medicinal residue again, the amount of water is half of the first time, boil and decoct at 100℃ for 30 minutes, then pour out the medicinal liquid and filter;

[0016] (3) Concentration:

[0017] Combine the two medicinal liquids, heat and concentrate on the fire, to a crude drug concentration of 1g / ml, pay attention to stirring during the process to prevent burning, after concentration, add sodium benzoate with a final concentration of 0.05% mass fraction for preservation, fill, seal, sterilize, and the Chinese herbal medicine oral liquid is completed.

[0018] The application of a traditional Chinese medicine composition in the preparation of a medicine for treating parainfluenza virus type 3 infection.

[0019] Scutellaria, also known as Camellia root and earth gold tea root, is bitter and cold in nature, and is related to the lungs, gallbladder, spleen, large intestine and small intestine. It mainly contains flavonoids such as scutellarein, baicalein and hanbaikalide. It can inhibit Staphylococcus aureus, Escherichia coli, Candida albicans and influenza virus and other pathogenic microorganisms. It has the effects of clearing heat and drying dampness, purging fire and detoxifying, stopping bleeding and calming the fetus, and is suitable for treating damp-heat diarrhea, lung heat cough, high fever, blood heat vomiting and blood loss, and fetal movement instability.

[0020] Terminalia chebula, also known as Terminalia chebula, is bitter, sour and astringent in nature, and is related to the lungs and large intestine. The main components include tannins, chebulagic acid and gallic acid. It has inhibitory effect on Staphylococcus aureus, Shigella and other pathogenic microorganisms. It has the effects of astringing the intestines to stop diarrhea, astringing the lungs to stop coughing, reducing fire and benefiting the throat, and is suitable for treating chronic diarrhea and chronic diarrhea, blood loss, anal prolapse, lung deficiency cough, chronic cough, sore throat and hoarseness.

[0021] Radix Astragali, also known as cotton, Huangqi, Dushen, sweet, slightly warm, spleen, lung. Mainly contains astragalus saponins, polysaccharides, flavonoids and amino acids and other ingredients. On Staphylococcus aureus, Streptococcus pneumoniae, Helicobacter pylori and other pathogenic microorganisms have a certain inhibitory effect. With Bubbling gas, solid, diuretic, pus, astringent wound healing effect, suitable for gas deficiency, food less loose, middle, chronic diarrhea, prolapse, self-hemorrhoids, edema and sores, sore, or long-term wound healing and other symptoms.

[0022] Honeysuckle, also known as Lonicera japonica, Shuanghua, Erbao flower, sweet, cold, lungs, heart, stomach. Mainly contains chlorogenic acid, luteolin, isochlorogenic acid, flavonoids and volatile oil and other ingredients. Can inhibit Staphylococcus aureus, hemolytic streptococcus, Escherichia coli, influenza virus, herpes simplex virus and other pathogenic microorganisms. With heat and detoxification, dispersing wind-heat effect, suitable for wind-heat cold, warm fever, sore throat, carbuncle, sores, toxic blood dysentery and wind-heat eye redness and other symptoms.

[0023] Forsythia, also known as Hualianzi, empty Kao, Lianke, bitter, slightly cold, lungs, heart, small intestine. Mainly contains forsythoside, forsythoside, volatile oil, flavonoids and other ingredients. Can inhibit Staphylococcus aureus, hemolytic streptococcus, Escherichia coli, influenza virus, herpes simplex virus and other pathogenic microorganisms. With heat and detoxification, swelling and dispersing wind-heat effect, suitable for wind-heat cold, warm fever, sore throat, carbuncle, sores, scrofula, hot and sore pain and erysipelas and other symptoms.

[0024] Radix Pulsatillae, also known as Guan Jie, Guan Gu, Baotou, Caotou, bitter, slightly cold, liver, lung, stomach. Mainly contains flavonoids, triterpenoid saponins, m-benzenetriol (such as Guanzhong), volatile oil and tannins and other ingredients. Can inhibit Staphylococcus aureus, Escherichia coli, influenza virus, herpes simplex virus and some fungi. With heat and detoxification, cooling blood and hemostasis, insecticidal effect, suitable for wind-heat cold, warm fever, blood heat and vomit, hematochezia, abdominal pain, also for sore and sores, burns and sores and leucorrhea and other symptoms.

[0025] Largeleaf privet leaf, also known as Dacian, Beiban Gen, Indigo root, bitter, cold, heart, lung, stomach. Mainly contains indigo, indigo, tryptamine ketone, flavonoids and organic acids and other ingredients. Can inhibit Staphylococcus aureus, hemolytic streptococcus, influenza virus, mumps virus and some fungi. With heat and detoxification, cooling blood and clearing effect, suitable for warm fever, convulsions, sore throat, erysipelas, sores, carbuncle, also for blood heat and vomit, mumps and viral pneumonia and other symptoms.

[0026] Licorice, also known as honey licorice, sweet grass, and licorice root, is sweet in taste and neutral in nature, and enters the heart, lung, spleen, and stomach meridians. It mainly contains glycyrrhizic acid, glycyrrhizin, glycyrrhizin flavonoids, volatile oils, and amino acids. It has a certain inhibitory effect on pathogenic microorganisms such as Staphylococcus aureus, Escherichia coli, Helicobacter pylori, and Candida albicans. It has the effects of tonifying the spleen and replenishing qi, clearing heat and detoxifying, resolving phlegm and relieving cough, relieving spasms and pain, and harmonizing other medicines. It is suitable for those with weak spleen and stomach, fatigue, palpitations, shortness of breath, cough with excessive phlegm, and abdominal cramps and pain. It is also often used as a harmonizing agent in compound prescriptions.

[0027] As can be seen from the above technical solution, compared with the prior art, the beneficial effects of the present invention are as follows:

[0028] BPIV3 infection is a respiratory infectious disease in cattle, and affected cattle often exhibit fever and respiratory symptoms. From the perspective of traditional Chinese veterinary medicine, its etiology is attributed to the invasion of external wind-heat pathogens into the cattle's body, leading to impaired lung function and imbalance of defensive qi, thus triggering a series of symptoms. Improper cattle feeding and management, such as damp pens, poor ventilation, and excessive labor, result in insufficient vital energy and weakened defenses, making the cattle susceptible to external pathogens.

[0029] Based on this, the formulation design of this invention is based on the following:

[0030] 1. Differentiation of syndromes based on pathogenic factors

[0031] Wind-heat invading the lungs: In the early stages of bovine parainfluenza virus type 3 infection, symptoms often present as wind-heat invading the lungs, such as fever, cough, and runny nose with clear or thick nasal discharge. The formula contains honeysuckle and forsythia to disperse wind-heat, clear heat and detoxify, effectively dispelling wind-heat pathogens from the lung meridian, allowing lung qi to flow smoothly, and relieving symptoms such as fever and cough; Isatis leaf clears heat and detoxifies, cools the blood and soothes the throat, and has a good effect on symptoms such as sore throat caused by wind-heat attacking the lungs, and can reduce respiratory inflammatory response.

[0032] Excessive Heat and Toxins: As the disease progresses, signs of excessive heat and toxins are likely to appear, such as persistent high fever, worsening cough, and thick, yellow phlegm. Scutellaria baicalensis effectively clears lung heat and toxins from the upper burner, significantly reducing heat in the lungs and alleviating various symptoms caused by excessive lung heat. Dryopteris crassirhizoma clears heat and detoxifies, cools the blood and stops bleeding; it can assist Scutellaria baicalensis and other herbs in enhancing their heat-clearing and detoxifying effects, helping to eliminate toxins from the body and control disease progression.

[0033] Astringent and antitussive effects: Terminalia chebula has the effects of astringing the intestines to stop diarrhea and astringing the lungs to stop cough. In bovine parainfluenza virus type 3 infection, cough symptoms often occur. Terminalia chebula can relieve cough by astringing lung qi, and is especially suitable for chronic cough and cough due to lung deficiency. It can prevent the dissipation of lung qi and allow it to be astringed, thereby reducing the severity and frequency of cough, and plays an important role in relieving respiratory symptoms.

[0034] 2. Differentiation of syndromes based on the body's vital energy.

[0035] Strengthening the body resistance and eliminating pathogenic factors: After the bovine is infected by parainfluenza virus type 3, the body resistance is easy to be weak, and the disease resistance is decreased. Huangqi has the effects of tonifying qi and ascending yang, and benefiting the defensive phase and consolidating the superficial phase, which can supplement the body resistance, enhance the disease resistance of the bovine, improve the defense function of the body, make the body resistance sufficient to eliminate pathogenic factors, and help the recovery in the later stage of the disease and prevent the disease from recurring.

[0036] 3. Overall conditioning and syndrome differentiation

[0037] Harmonizing the drug properties: Gancao is the key drug for harmonizing the properties of various drugs, which can moderate the properties of various drugs in the prescription, make the various drugs play the synergistic effect, and also has the effects of clearing heat and resolving toxins, moistening the lung and relieving cough, which can relieve the cough symptoms, and play a certain buffering effect on the side effects of the drugs, protect the function of the spleen and stomach, and make the whole prescription more gentle and effective.

[0038] The traditional Chinese medicine composition is aimed at the infection of the bovine parainfluenza virus type 3, and can regulate the body from the aspects of clearing heat and resolving toxins, dispersing wind-heat, astringing the lung and relieving cough, strengthening the body resistance and eliminating pathogenic factors, and harmonizing the drug properties, so as to restore the balance of the body's yin and yang, qi and blood, and the functions of the viscera, and achieve the purpose of treating the disease. DETAILED DESCRIPTION

[0039] The technical solutions in the embodiments of the present application will be clearly and completely described below. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all the other embodiments obtained by those skilled in the art without creative labor fall within the protection scope of the present application.

[0040] Embodiment 1

[0041] A traditional Chinese medicine composition for treating bovine parainfluenza virus type 3 infection, the traditional Chinese medicine composition comprises the following components in parts by weight:

[0042] Huangqi 6 parts, Huangqi 5 parts, Hezi 5 parts, Jinyinhua 3 parts, Lianqiao 3 parts, Guanzhong 2 parts, Daqingye 1 part, and Gancao 1 part.

[0043] Powder preparation method:

[0044] (1) Prepare the medicinal materials, wash the surface of the medicinal materials with flowing water to remove the dirt and impurities, and dry at 56℃.

[0045] (2) Weigh the medicinal materials according to the proportion, use a hammer crusher or a universal crusher to crush the dried medicinal materials into coarse powder with a particle size of about 2-5mm; further use a supermicro grinder (such as airflow crushing, vibration grinding) to process the coarse powder into fine powder. Screen with a standard sieve of 100 meshes to ensure uniform powder particle size.

[0046] (3) Further mix the Chinese herbal medicine powder in a three-dimensional motion mixer for 30 minutes. After mixing, pack in aluminum plastic packaging bags.

[0047] Oral liquid preparation method:

[0048] (1) Preparation of medicinal materials, according to the proportion of medicinal materials, remove impurities, crush into coarse powder, soak for 2h before decoction.

[0049] (2) Decoction: mix the medicinal materials with water according to the mass ratio of 1:5, first boil with strong fire, then turn to medium heat 100℃ and decoct for 30 minutes. After decoction, pour out the medicinal liquid, filter with gauze. Add water to the medicinal residue again, the water amount is about half of the first time, boil with strong fire, then decoct with medium heat for 30 minutes, then pour out the medicinal liquid and filter.

[0050] (3) Concentration: combine the two medicinal liquids, heat and concentrate on the fire, to the concentration of crude drug 1g / ml, pay attention to stirring during the period, prevent scorching. After concentration, add sodium benzoate with a final concentration of 0.05% mass fraction for preservation, then fill, seal, sterilize, and the Chinese herbal medicine oral liquid is completed.

[0051] Example 2

[0052] A traditional Chinese medicine composition for treating bovine parainfluenza virus type 3 infection, the traditional Chinese medicine composition comprises the following components by weight fraction:

[0053] Scutellaria 5 parts, Astragalus 4 parts, Terminalia 4 parts, honeysuckle 2 parts, forsythia 2 parts, 2 parts of rhinacanthus, 1 part of isatis, 1 part of licorice.

[0054] The preparation method is the same as example 1.

[0055] Example 3

[0056] A traditional Chinese medicine composition for treating bovine parainfluenza virus type 3 infection, the traditional Chinese medicine composition comprises the following components by weight fraction:

[0057] Scutellaria 7 parts, Astragalus 6 parts, Terminalia 6 parts, honeysuckle 3 parts, forsythia 3 parts, 3 parts of rhinacanthus, 2 parts of isatis, 2 parts of licorice.

[0058] The preparation method is the same as example 1.

[0059] In vitro inhibition test of the traditional Chinese medicine composition prepared in example 1 on BPIV3 virus

[0060] The present application utilizes BPIV3 infected MDBK cell model to evaluate the inhibition effect of the traditional Chinese medicine composition on BPIV3 from effective inhibition rate and virus titer determination.

[0061] ①Determination of the maximum safe concentration of Chinese herbal medicine.

[0062] The present application uses IBRV infection of MDBK cell model as the basis for evaluating the effect of Chinese herbal medicine. Since high-dose Chinese herbal medicine itself can inhibit or damage the cells, the present application first evaluates the toxic damage of the Chinese medicine composition to MDBK cells, and the highest drug concentration without inhibition and damage to the growth of MDBK cells is used as the maximum safe concentration. Specifically, the MDBK cells are inoculated in a 96-well cell culture plate, and when the cell density reaches 60%, different dilution multiples of Chinese herbal medicine oral liquid are added to the cell culture space, and different dilution multiples of ribavirin are used as a control group. The degree of cell pathological changes (CPE) caused by IBRV and the number of viable cells in the cell well are evaluated by CCK-8 kit to evaluate the toxicity of the drug. The results show that the Chinese medicine composition and ribavirin both have certain inhibition and toxicity to MDBK cells, and the effect shows a certain dose dependence. The maximum safe concentration of ribavirin is 2 mg / mL, and the maximum safe concentration of the Chinese medicine composition of the present application is 3.125 mg / mL.

[0063] ②Evaluation of the effective inhibition rate of Chinese herbal medicine on IBRV.

[0064] The single Chinese herbal medicine with the maximum safe concentration is continuously diluted by 2 times, 50 μL of the diluted solution and 50 μL of 100 TCID50 virus solution are mixed, and then the mixture is added to the 96-well cell culture plate. The treated 96-well cell culture plate is placed in a 37°C carbon dioxide incubator overnight for culture. The number of cell pathological changes and the degree of pathological changes in each well of the 96-well plate are recorded and observed under a microscope.

[0065] The CPE changes of the cells in the well are observed under a microscope, and the culture is stopped when the CPE no longer continues. After washing with PBS washing solution for 3 times in a clean bench, 10 μL of CCK-8 reagent is added to each well, and the cells are incubated in a 37°C carbon dioxide incubator for 4 hours. Then the OD value of the cells is measured at 450 nm wavelength, and the effective inhibition rate of the drug is calculated according to the formula after the result is determined.

[0066] Effective inhibition rate of drug (%) = [(average OD value of the test drug group - OD value of the virus control group) / (average OD value of the cell control group - OD value of the virus control group)] x 100%.

[0067] The results show that the Chinese medicine composition and ribavirin of the present application both have inhibitory effect on the replication of BPIV3 in MDBK cells, and show a certain dose-dependent relationship, i.e. the inhibitory effect is best at the maximum safe concentration, and when the concentration is lower than the maximum safe concentration, the higher the cell pathological rate is. Overall, the effective inhibition rate of the Chinese medicine composition of the present application on BPIV3 is higher than that of ribavirin.

[0068] Table 1: Effective inhibition rate of BPIV3 by oral liquid of Chinese medicine composition in Example 1 and ribavirin

[0069] Drug dilution factor Pharmaceutical composition Ribavirin 2 0 ]] 66.3 54.7 2 -1 ]] 42.3 32.9 2 -2 ]] 26.2 21.8 2 -3 ]] 21.4 12.9 2 -4 ]] 17.5 4 C 100 100.00 V 0 0.00

[0070] C: cell control group; V: virus control group

[0071] ③ Determination and evaluation of intervention effect of single Chinese herbal medicine on BPIV3 replication

[0072] Similarly, the BPIV3 virus infected cells were treated with Chinese herbal medicine, and the virus was harvested to determine the virus TCID50. The results showed (Table 2) that after treatment with ribavirin and the Chinese medicine composition of the present application, the replication of BPIV3 in MDBK cells was significantly inhibited, and the inhibition effect of the Chinese herbal medicine treatment group was more obvious, and the virus titer produced by the infected cells was lower.

[0073] Table 2: Inhibition effect of BRDC and BED viral pathogens by oral liquid of Chinese medicine composition in Example 1

[0074] Virus + drug TCID50 Virus + self-made Chinese herbal medicine 6.37 Virus + ribavirin 7.42 Virus control 8.23 Cell control -

[0075] In vitro inhibition test of Chinese medicine composition on BPIV3 virus

[0076] Beef BPIV3 infection cases were provided by breeders in Changli County, Hebei Province and Weicheng County, Chengde City. According to the clinical symptoms and pathogenic examination results, it was diagnosed as BPIV3. The Chinese medicine composition of the present application was used as the test group, and the veterinary antiviral drug Banqing Granules (Chinese Veterinary Pharmacopoeia 2005 Edition Part 2) was used as the control group. The sick cattle were treated, and the cure rate was calculated. The treatment results are shown in Tables 3 and 4.

[0077] Drug standard: Sick poultry mixed the Chinese herbal medicine oral liquid in water at a dose of 50 ml per bird, and drank or gavage once a day, 5 days as a course of treatment. Banqing Granules 0.2 g / kg body weight, the drug was dissolved in water, morning and evening each time, gavage or drinking water. In addition to antiviral treatment, the control group and the experimental group also added the same standard antibiotic treatment to control the induced infection.

[0078] Cure rate determination standard: After the end of treatment, continue to observe for 3-5 days, and those with clinical symptoms disappearing, drinking water, feeding and mental state returning to normal are judged as cured, and the prevention and treatment effect is determined combined with the pathological examination results, and the cure rate is calculated.

[0079] Table 3: Clinical treatment effect of oral liquid of Chinese medicine composition in Example 1 on BPIV3 infection in cattle

[0080]

[0081] Table 4 Treatment effect of Banqing granules on bovine BPIV3 infection

[0082]

[0083] Through the treatment of BPIV3 infection cases in different regions, the Chinese herbal medicine oral liquid of the present application has a significant treatment effect on the disease, with an average cure rate of 98%, and the symptoms are generally relieved on the second day after treatment, disappear after 4-7 days, and the normal feeding is restored; the average cure rate of the Banqing granule control group treating natural cases is 92%, and some cattle still have respiratory symptoms after 7 days of treatment. In terms of treatment time, early detection and treatment is crucial for BPIV3 infection, which can reduce the harm of the disease. Overall, the treatment effect of the Chinese medicine composition of the present application on BPIV3 is better than that of the Banqing granule control group.

[0084] The above description of disclosed embodiments enables one of ordinary skill in the art to make or use the application. Various modifications to these embodiments will be readily apparent to those of ordinary skill in the art, and the generic principles defined herein can be applied to other embodiments without departing from the spirit or scope of the application. Therefore, the present application is not to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A traditional Chinese medicine composition for treating bovine parainfluenza virus type 3 infection, characterized in that, The traditional Chinese medicine composition comprises the following components in parts by weight: Scutellaria baicalensis 5-7 parts, Astragalus membranaceus 4-6 parts, Terminalia chebula 4-6 parts, Lonicera japonica 2-3 parts, Forsythia suspensa 2-3 parts, Dryopteris crassirhizoma 2-3 parts, Isatis tinctoria 1-2 parts, Glycyrrhiza uralensis 1-2 parts.

2. The traditional Chinese medicine composition according to claim 1, characterized in that, The dosage form of the traditional Chinese medicine composition is powder or oral liquid.

3. The method for preparing the traditional Chinese medicine composition according to claim 2, characterized in that, The preparation of the oral liquid includes the following steps: (1) Preparation of medicinal materials: Weigh the medicinal materials according to the proportion, remove impurities, crush them into coarse powder, and soak them in water for 2 hours. (2) Decoction: Mix the herbs and water in a 1:5 mass ratio, boil and then decoct at 100℃ for 30 minutes; after decoction, pour out the liquid and filter with gauze; add water to the dregs again, half the amount of water added the first time, boil and then decoct at 100℃ for 30 minutes, then pour out the liquid and filter. (3) Concentration: Combine the two medicinal solutions, heat and concentrate to a crude drug concentration of 1g / ml, stirring constantly to prevent scorching. After concentration, add sodium benzoate at a final concentration of 0.05% by mass for preservation, fill, seal, and sterilize. The oral liquid of traditional Chinese medicine is now ready.

4. The use of the traditional Chinese medicine composition according to any one of claims 1-2 in the preparation of a drug for treating bovine parainfluenza virus type 3 infection.

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