A composition with antioxidant and whitening effects, and a preparation method and use thereof

Abstract

The application belongs to the technical field of food processing, and specifically provides a composition with antioxidant and whitening effects, a preparation method and uses thereof. The composition with antioxidant and whitening effects contains the following raw material components by weight: 20-40 parts of glutathione-rich yeast extract, 0.5-5 parts of burdock root extract, 0.5-5 parts of cordyceps militaris extract, 0.2-2 parts of white pine mushroom freeze-dried powder, 0.2-2 parts of boletus concentrate powder, and 20-40 parts of osmanthus powder. The composition provided by the technical scheme is compounded according to a certain proportion, so that it has good antioxidant and whitening effects.

Description

Technical Field

[0001] This invention relates to the field of food processing technology, and in particular to a composition with antioxidant and whitening effects, its preparation method, and its uses. Background Technology

[0002] With the rapid development of modern society and the economy, people's living standards and health awareness have also increased, and their pursuit of beauty has become more comprehensive. Skin problems (such as dull skin, age spots, wrinkles, and sagging) have become one of the primary concerns, mainly due to oxidative stress from excessive free radicals and excessive melanin production. In addition to external skincare products, people are increasingly inclined towards "inside-out" skincare.

[0003] Glutathione is a tripeptide composed of L-glutamic acid, glycine, and L-cysteine. It is widely found in plants, animals, and microorganisms and is one of the most important non-protein thiol compounds in living organisms. Glutathione (GSH) is an abundant non-protein thiol in cells and one of the most abundant antioxidants in the human body. It participates in redox reactions within the body and has various physiological functions.

[0004] Glutathione-enriched yeast is a functional yeast product made from Saccharomyces cerevisiae using modern biotechnology through cultivation, fermentation, enzymatic hydrolysis, centrifugation (or non-centrifugation), and drying. It belongs to the category of common food ingredients. Essentially, it consists of yeast cells rich in glutathione (GSH) and has broad market prospects in areas such as oral beauty treatments and hangover relief and liver protection.

[0005] Existing technologies disclose several food products for antioxidant and skin whitening purposes. For example, CN 115349636 A discloses a skin whitening and spot-fading composition containing bird's nest peptides. This composition has the effects of removing or fading facial spots and whitening the skin. It also has excellent anti-glycation effects, maintaining skin elasticity, delaying skin aging, and reducing wrinkles. CN108434443 B discloses a skin whitening and antioxidant composition and its uses. This composition can effectively replenish the collagen lost by the skin, thereby restoring the skin's elasticity and hydration. It can also prevent ultraviolet rays from damaging skin tissue, thus ensuring normal skin metabolism. At the same time, the astaxanthin in Haematococcus pluvialis extract has an absorption wavelength similar to UVA, which can significantly absorb long-wave ultraviolet rays, further reducing the damage caused by ultraviolet rays to the skin. It can also inhibit the melanin synthesis pathway.

[0006] However, there is still room for improvement in the antioxidant and whitening effects of the aforementioned patents, and the use of glutathione-enriched yeast is still in its initial stage. Therefore, in response to the current market demand for antioxidant and whitening products, developing a food-medicine homologous product containing glutathione-enriched yeast extract that also has antioxidant and whitening effects is a technical problem that urgently needs to be solved by those skilled in the art. Summary of the Invention

[0007] In view of the shortcomings of the prior art described above, the purpose of this invention is to provide a composition with antioxidant and whitening effects, its preparation method and uses, in order to solve the problems in the prior art.

[0008] To achieve the above and other related objectives, the present invention is obtained through the following technical solution.

[0009] The first aspect of the present invention provides a composition having antioxidant and whitening effects, comprising at least the following raw material components in parts by weight: 20-40 parts of glutathione-rich yeast extract, 0.5-5 parts of burdock root extract, 0.5-5 parts of cordyceps extract, 0.2-2 parts of freeze-dried white matsutake mushroom powder, 0.2-2 parts of concentrated porcini mushroom powder, and 20-40 parts of osmanthus powder.

[0010] The glutathione-enriched yeast extract can be in the form of 25-35 parts, 20-32 parts, 25-40 parts, 26-32 parts, 26-30 parts, or 28-32 parts; the burdock root extract can be in the form of 2-5 parts, or 2 parts, 3 parts, 4 parts, or 5 parts; the cordyceps militaris extract can be in the form of 2-4 parts, or 2 parts, 3 parts, or 4 parts; the white matsutake freeze-dried powder can be in the form of 0.5-1.5 parts, or 0.5 parts, 1.0 parts, or 1.5 parts; the porcini concentrated powder can be in the form of 0.5-1.5 parts, or 0.5 parts, 1.0 parts, or 1.5 parts; the osmanthus powder can be in the form of 25-35 parts, 20-32 parts, 25-40 parts, 26-32 parts, 26-30 parts, or 28-32 parts.

[0011] Preferably, the raw materials of the composition further include bird's nest peptides, and the weight ratio of the glutathione-enriched yeast extract to the bird's nest peptides is 20-40:1-10. For example, the weight ratio of the glutathione-enriched yeast extract to the bird's nest peptides can be 20-40:2-5, 25-35:2-5, 25-35:2, 25-35:3, 25-35:4, or 25-35:5.

[0012] Preferably, the composition comprises the following raw material components in parts by weight: 25-35 parts of glutathione-rich yeast extract, 2-5 parts of burdock root extract, 2-4 parts of cordyceps extract, 2-5 parts of bird's nest peptide, 0.5-1.5 parts of freeze-dried white matsutake mushroom powder, 0.5-1.5 parts of concentrated porcini mushroom powder, and 25-35 parts of osmanthus powder.

[0013] The glutathione-enriched yeast extract can be in quantities of 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, or 35 parts; the burdock root extract can be in quantities of 2, 3, 4, or 5 parts; the cordyceps militaris extract can be in quantities of 2, 3, or 4 parts; the bird's nest peptide can be in quantities of 2, 3, 4, or 5 parts; the white matsutake freeze-dried powder can be in quantities of 0.5, 1.0, or 1.5 parts; the porcini mushroom concentrate powder can be in quantities of 0.5, 1.0, or 1.5 parts; and the osmanthus powder can be in quantities of 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, or 35 parts.

[0014] Preferably, the glutathione content in the glutathione-enriched yeast extract is 15-25 wt%. For example, it can be 15-20 wt% or 20-25 wt%.

[0015] Preferably, the sialic acid content in the bird's nest peptide is 1-5 wt%; more preferably, it is 1-3 wt%. For example, it can be 1 wt%, 2 wt%, and 3 wt%.

[0016] Preferably, the polysaccharide content in the burdock root extract is 25-35 wt%. For example, it can be 28-32 wt%, specifically 28 wt%, 29 wt%, 30 wt%, 31 wt%, and 32 wt%.

[0017] Preferably, the polysaccharide content in the Cordyceps militaris extract is 25-35 wt%. For example, it can be 28-32 wt%, specifically 28 wt%, 29 wt%, 30 wt%, 31 wt%, and 32 wt%.

[0018] Preferably, the content of matsutake polysaccharide in the freeze-dried white matsutake powder is 25-35 wt%. For example, it can be 28-32 wt%, specifically 28 wt%, 29 wt%, 30 wt%, 31 wt%, or 32 wt%.

[0019] Preferably, the ergothioneine content in the porcini mushroom concentrate is 0.5-3 wt%. For example, it can be 0.5-2 wt%, 1.2-1.8 wt%, 1.3 wt%, 1.4 wt%, 1.5 wt%, 1.6 wt%, 1.7 wt%, or 1.8 wt%.

[0020] Preferably, the glutathione content in the porcini mushroom concentrate is 0.01~0.5 wt%. For example, it can be 0.1~0.3 wt%, or 0.1 wt%, 0.2 wt%, or 0.3 wt%.

[0021] Preferably, the composition has an ABTS radical scavenging rate of 60-90% and a DPPH radical scavenging rate of 60-85%.

[0022] The free radical scavenging rate of ABTS can be 66~83%, 65~85%, or 63~88%; the free radical scavenging rate of DPPH can be 69~79%, 65~85%, or 68~80%.

[0023] The second aspect of the present invention provides a method for preparing the composition with antioxidant and whitening effects as described above, comprising the following steps: mixing the glutathione-rich yeast extract, burdock root extract, cordyceps extract, white matsutake freeze-dried powder, porcini concentrated powder and osmanthus powder evenly to obtain the composition.

[0024] Preferably, bird's nest peptides are also added during mixing.

[0025] Preferably, the mixture is dried after mixing, and the drying temperature is 30~50℃, and the drying time is 1~3h. For example, the drying temperature can be 30~40℃ or 33~38℃; the drying time can be 1h, 2h, or 3h.

[0026] A third aspect of the present invention provides the use of the composition having antioxidant and whitening effects as described above in the preparation of antioxidant products.

[0027] A fourth aspect of the present invention provides a product having antioxidant properties, comprising the composition having antioxidant and whitening properties as described above and food-grade acceptable excipients.

[0028] Preferably, the excipients are selected from one or more of sweeteners, acid regulators, fillers, flavoring agents, colorants, antioxidants, thickeners, stabilizers, emulsifiers, anti-caking agents, flow aids, and lubricants.

[0029] Preferably, the excipients include maltodextrin.

[0030] This application does not specify a particular amount of maltodextrin; the amount can be selected based on the actual type and intended use of the product. For example, the weight ratio of maltodextrin to glutathione-enriched yeast extract can be 0.5–5:1, 1–3:1, or 1.5–2:1.

[0031] Preferably, the product is selected from one or more of solid beverages, liquid beverages, dairy products and flavoring powders, and is preferably a solid beverage.

[0032] Beneficial effects:

[0033] This invention combines yeast extract rich in glutathione, burdock root extract, cordyceps extract, bird's nest peptide, freeze-dried white matsutake powder, concentrated porcini mushroom powder, and osmanthus powder in a certain proportion to obtain a composition with good antioxidant and whitening effects.

[0034] This composition, with the synergistic effect of glutathione-rich yeast extract and osmanthus powder as its core, forms the basic line of defense for skin's anti-oxidation and whitening. The glutathione-rich yeast extract provides the body with an appropriate amount of glutathione, a powerful endogenous oxidant that can enhance SOD activity and strengthen the body's own antioxidant capacity, thereby improving the cells' ability to resist oxidative damage from within. Osmanthus powder, rich in flavonoids (such as quercetin) and polyphenols, can directly scavenge free radicals and has a certain metal ion chelating ability. The exogenous direct scavenging of osmanthus powder and the endogenous enzymatic defense of the glutathione-rich yeast extract complement each other, working synergistically to cover different sites and stages of free radical generation.

[0035] In addition, flavonoids in osmanthus pollen have been shown to inhibit tyrosinase activity, which can reduce melanin production at its source; while small molecules such as oligopeptides in glutathione-rich yeast extract can compete for the action site of tyrosinase. The two work synergistically to block key steps in melanin synthesis and reduce the number of melanocytes.

[0036] Meanwhile, the synergistic effect of burdock root extract, cordyceps militaris extract, freeze-dried white matsutake mushroom powder, and concentrated porcini mushroom powder in the composition strengthens the skin's antioxidant and whitening defenses. It not only effectively scavenge free radicals and enhances the antioxidant system, but also has anti-inflammatory effects, reducing skin inflammation.

[0037] Specifically, the antioxidant components such as arctiin and chlorogenic acid in burdock root extract, along with the effective components such as cordyceps polysaccharide, cordycepin, ergosterol and its derivatives in cordyceps extract, and the polyphenolic antioxidant components such as matsutake polysaccharide in white matsutake freeze-dried powder and ergothioneine and chlorogenic acid in porcini concentrated powder, work synergistically to form a synergistic antioxidant network that can neutralize various types of free radicals.

[0038] Furthermore, bird's nest peptides not only help accelerate skin cell repair, but their small molecule polypeptides and sialic acid also synergistically work with the active ingredients in the basic and reinforced antioxidant defenses, further enhancing the antioxidant and anti-inflammatory effects of the composition. Simultaneously, they nourish the skin and improve its radiance. Moreover, the active ingredients in matsutake polysaccharides, porcini mushroom concentrate powder, bird's nest peptides, and osmanthus powder (linalool, geraniol) possess anti-inflammatory activity, inhibiting the release of inflammatory factors such as TNF-α, IL-6, IL-1β, and IL-18, thus alleviating skin inflammation.

[0039] The antioxidant composition provided by this invention has components that work together to enhance the overall effect, resulting in a composition that effectively improves skin oxidation and dullness, and provides excellent antioxidant, anti-inflammatory, and whitening cosmetic benefits. Detailed Implementation

[0040] The following specific embodiments illustrate the implementation of the present invention. Those skilled in the art can easily understand other advantages and effects of the present invention from the content disclosed in this specification.

[0041] Before further describing specific embodiments of the present invention, it should be understood that the scope of protection of the present invention is not limited to the specific embodiments described below; it should also be understood that the terminology used in the embodiments of the present invention is for describing specific embodiments and not for limiting the scope of protection of the present invention. Test methods in the following embodiments that do not specify specific conditions are generally performed under conventional conditions or as recommended by the respective manufacturers.

[0042] When numerical ranges are given in the embodiments, it should be understood that, unless otherwise stated in the present invention, both endpoints of each numerical range and any value between the two endpoints may be selected. Unless otherwise defined, all technical and scientific terms used in this invention have the same meaning as commonly understood by one of ordinary skill in the art. In addition to the specific methods, apparatus, and materials used in the embodiments, based on the knowledge of the prior art possessed by one of ordinary skill in the art and the description of this invention, any prior art methods, apparatus, and materials similar to or equivalent to those described, apparatus, and materials in the embodiments of this invention may be used to implement the present invention.

[0043] Glutathione-enriched yeast is a functional yeast product made from Saccharomyces cerevisiae using modern biotechnology through cultivation, fermentation, enzymatic hydrolysis, centrifugation (or non-centrifugation), and drying. It is a common food ingredient. Its source is safe, and its essence is yeast cells rich in glutathione (GSH). Glutathione-enriched yeast extract is a food ingredient obtained from glutathione-enriched yeast through modern food processing technologies such as enzymatic hydrolysis, autolysis, enrichment, extraction, and refining. It contains glutathione, protein, various amino acids, vitamins, minerals, and other nutrients. Among these, the characteristic component glutathione (GSH) is a tripeptide composed of glutamic acid, cysteine, and glycine. It is one of the core components of the human body's antioxidant system, possessing important physiological functions such as anti-free radical activity, detoxification, immune enhancement, and liver protection.

[0044] Burdock root, the root of the burdock plant (Arctium lappa L.), belongs to the Asteraceae family. It is both a traditional Chinese medicine and a nutritious edible plant. The core components of burdock root extract include flavonoids such as arctiin and arctigenin, as well as polyphenols such as caffeic acid and chlorogenic acid, all of which are antioxidants. Burdock root extract has the ability to scavenge various free radicals, achieving its antioxidant effect by directly eliminating free radicals. Simultaneously, it can increase the activity of antioxidant enzymes such as superoxide dismutase (SOD) and glutathione peroxidase in the body, enhancing the antioxidant enzyme system and strengthening the cell's own defense capabilities. Furthermore, chlorogenic acid and other polyphenols, water-soluble dietary fiber, and other active ingredients work synergistically to exert antioxidant effects. It also possesses multiple health benefits, including antibacterial, anti-inflammatory, antioxidant, and immune-regulating properties.

[0045] Cordyceps militaris (L.ex Fr.) Link. is a fungus belonging to the family Clavicipitaceae and the genus Cordyceps. Also known as North Cordyceps, it is a dual-purpose edible and medicinal fungus with a protein content as high as 40.7%. It contains a complete range and abundant quantity of essential amino acids, and artificially cultivated Cordyceps militaris also contains rich vitamins and minerals. The core components of Cordyceps militaris extract include cordycepin, Cordyceps militaris polysaccharide, ergosterol, and various amino acid and protein components. Cordyceps militaris polysaccharide has the ability to scavenge DPPH, superoxide anions (O2⁻), hydroxyl radicals (·OH), and hydrogen peroxide (H2O2), directly eliminating free radicals. Simultaneously, combined with cordycepin, ergosterol, and their derivatives, it directly exerts antioxidant effects, protecting cells from oxidative stress damage, thus achieving antioxidant, anti-inflammatory, and anti-aging effects.

[0046] Bird's nest is a nest built by swiftlets (Apodidae) and other related species, using a mixture of saliva and down feathers. Bird's nest peptides, on the other hand, are small-molecule active peptides extracted and separated from bird's nest through enzymatic hydrolysis of large-molecule bird's nest protein. Their main active substances include small-molecule polypeptides and sialic acid. The small-molecule polypeptides and sialic acid in bird's nest peptides can promote cell regeneration and repair, thereby accelerating tissue repair and delaying aging. They also have the ability to scavenge free radicals, directly neutralizing reactive oxygen species (ROS) that cause skin aging and activating the core antioxidant pathway—the Nrf2 pathway. In addition, bird's nest peptides can inhibit the activity of tyrosinase, reducing the "raw materials" for melanin production at the source, and suppressing inflammatory responses. Therefore, they possess antioxidant, anti-inflammatory, and whitening effects.

[0047] The freeze-dried white matsutake mushroom powder in this application is a granular substance prepared from white matsutake mushrooms (Tuber magnatum) using vacuum freeze-drying technology. It fully retains the active ingredients of white matsutake mushrooms, such as matsutake polysaccharides, matsutake alcohol, and matsutake polypeptides. The freeze-dried white matsutake mushroom powder not only enhances the body's immunity but also, through the synergistic effect of matsutake polysaccharides and polypeptides, scavenges DPPH, superoxide anions, and hydroxyl free radicals, exhibiting antioxidant and anti-aging effects. Furthermore, the freeze-dried white matsutake mushroom powder can inhibit the release of inflammatory factors and reduce skin inflammation.

[0048] Boletus, the fruiting body of the fungi Boletus speciosus Frost [Suillusspeciosus (Frost) Kuntz.], Boletus queletii Schulz., and Boletus regius Krombh. (family Boletaceae), is a highly nutritious edible fungus, rich in protein, B vitamins, potassium, iron, and other elements, as well as dietary fiber and antioxidants (vitamin E and polyphenols). Boletus concentrate powder, on the other hand, is made from fresh, edible Boletus mushrooms using modern food processing techniques (such as extraction, concentration, spray drying, and sterilization) to highly concentrate the essential components of the fresh mushrooms. It is enriched with the bioactive components of Boletus, such as Boletus polysaccharides, vitamin E, ergothioneine, and polyphenols. Boletus edulis concentrate not only regulates the immune system and improves gut microbiota imbalance, but also effectively scavenge free radicals and protect cell membranes, mitochondria, and DNA from oxidative damage through polyphenols such as ergothioneine and chlorogenic acid, as well as antioxidants like vitamin E, exhibiting powerful antioxidant and anti-aging effects. Furthermore, Boletus edulis concentrate can inhibit the expression of inflammatory factors and has inhibitory effects on various bacteria and fungi. It possesses multiple benefits, including antioxidant, antibacterial, anti-inflammatory, gut microbiota regulation, immune enhancement, and metabolic improvement.

[0049] Osmanthus powder is a powdered product made from fresh or dried osmanthus flowers (Osmanthus fragrans, a plant in the Oleaceae family) using traditional techniques or modern technology. It retains the inherent properties of osmanthus flowers and possesses multiple benefits, including warming the middle jiao (spleen and stomach), dispelling cold, resolving phlegm and relieving cough, and anti-oxidation and beautifying the skin. Among its components, flavonoids such as quercetin and kaempferol have powerful antioxidant properties, delaying cell aging, while volatile oils such as linalool and geraniol have anti-inflammatory and soothing effects. It also has beautifying and nourishing effects, improves liver blood, and reduces dull skin tone.

[0050] Unless otherwise specified, the raw materials used in the following embodiments and comparative examples of this application are all commercially available raw materials.

[0051] Among them: glutathione-rich yeast extract was purchased from Angel Yeast Co., Ltd., model KA66; burdock root extract was purchased from Hunan Aijia Biotechnology Co., Ltd.; cordyceps militaris extract was purchased from Hunan Aijia Biotechnology Co., Ltd.; bird's nest peptide was purchased from Guangzhou Fenwei Trading Co., Ltd.; osmanthus powder was purchased from Tianjin Zhenruguo Food Industry Co., Ltd.; porcini mushroom concentrate powder was purchased from Xi'an Nuozhong Kangjian Biotechnology Co., Ltd.; and white matsutake freeze-dried powder was purchased from Weizhuo Health Group.

[0052] In the following examples and comparative examples of this application, the glutathione content of the yeast extract KA66 enriched with glutathione is 15 wt%, the sialic acid content of the bird's nest peptide is 2 wt%, the ergothionein content of the porcini mushroom concentrate powder is 1.5 wt%, and the matsutake polysaccharide content of the white matsutake freeze-dried powder is 30 wt%.

[0053] All raw materials used in this application are commercially available and are in powder form.

[0054] Example 1

[0055] Antioxidant Compositions and Their Preparation Methods

[0056] Specifically, the steps are as follows: Take 26 parts of glutathione-rich yeast extract KA66, 4 parts of burdock root extract, 4 parts of cordyceps extract, 1 part of white matsutake freeze-dried powder, 1 part of porcini concentrated powder and 26 parts of osmanthus powder, mix them evenly, and dry them in an oven at 35°C for 2 hours to obtain the antioxidant composition.

[0057] Antioxidant solid beverages and their preparation methods

[0058] Specifically, the steps include: taking the antioxidant composition as described above and mixing it evenly with 50 parts of maltodextrin to obtain the antioxidant solid beverage.

[0059] Example 2

[0060] Antioxidant Compositions and Their Preparation Methods

[0061] Specifically, the steps are as follows: Take 25 parts of glutathione-rich yeast extract KA66, 3 parts of bird's nest peptide, 3 parts of burdock root extract, 3 parts of cordyceps extract, 1 part of white matsutake freeze-dried powder, 1 part of porcini concentrated powder and 25 parts of osmanthus powder, mix them evenly, and dry them in an oven at 35°C for 2 hours to obtain the antioxidant composition.

[0062] Antioxidant solid beverages and their preparation methods

[0063] Specifically, the steps include: taking the antioxidant composition as described above and mixing it evenly with 50 parts of maltodextrin to obtain the antioxidant solid beverage.

[0064] Example 3

[0065] Antioxidant Compositions and Their Preparation Methods

[0066] Specifically, the steps are as follows: Take 32 parts of glutathione-rich yeast extract KA66, 5 parts of bird's nest peptide, 5 parts of burdock root extract, 4 parts of cordyceps extract, 1.5 parts of white matsutake freeze-dried powder, 1.5 parts of porcini concentrated powder and 32 parts of osmanthus powder, mix them evenly, and then dry them in an oven at 35°C for 2 hours to obtain the antioxidant composition.

[0067] Antioxidant solid beverages and their preparation methods

[0068] Specifically, the steps include: taking the antioxidant composition as described above and mixing it evenly with 50 parts of maltodextrin to obtain the antioxidant solid beverage.

[0069] Example 4

[0070] Antioxidant Compositions and Their Preparation Methods

[0071] Specifically, the steps are as follows: Take 25 parts of glutathione-rich yeast extract KA66, 2 parts of bird's nest peptide, 2 parts of burdock root extract, 2 parts of cordyceps extract, 0.5 parts of white matsutake freeze-dried powder, 0.5 parts of porcini concentrated powder, and 25 parts of osmanthus powder, mix them evenly, and then dry them in an oven at 35°C for 2 hours to obtain the antioxidant composition.

[0072] Antioxidant solid beverages and their preparation methods

[0073] Specifically, the steps include: taking the antioxidant composition as described above and mixing it evenly with 50 parts of maltodextrin to obtain the antioxidant solid beverage.

[0074] Comparative Example 1

[0075] The only difference from Example 2 is that osmanthus powder is not added, the amount of glutathione-enriched yeast extract is increased to 50 parts, the total amount of raw materials remains unchanged, and all other conditions are the same.

[0076] Comparative Example 2

[0077] The only difference from Example 2 is that the glutathione-enriched yeast extract is not added, the amount of osmanthus powder is increased to 50 parts, the total amount of raw materials remains unchanged, and all other conditions are the same.

[0078] Comparative Example 3

[0079] The only difference from Example 2 is that burdock root extract is not added, the ratio of Cordyceps militaris extract, white matsutake freeze-dried powder and Boletus edulis concentrated powder is kept unchanged (3:1:1) and the amount is increased to a total of 8 parts, and all other conditions are the same.

[0080] Comparative Example 4

[0081] The only difference from Example 2 is that Cordyceps militaris extract is not added, the ratio of burdock root extract, white matsutake freeze-dried powder and porcini concentrated powder is kept unchanged (3:1:1) and the amount is increased to a total of 8 parts, and all other conditions are the same.

[0082] Comparative Example 5

[0083] The only difference from Example 2 is that Boletus edulis concentrate is not added, the ratio of burdock root extract, Cordyceps militaris extract and white matsutake freeze-dried powder is kept unchanged (3:3:1) and the amount is increased to a total of 8 parts, and all other conditions are the same.

[0084] Comparative Example 6

[0085] The only difference from Example 2 is that the freeze-dried white matsutake mushroom powder is not added, the ratio of burdock root extract, cordyceps militaris extract and porcini mushroom concentrate is kept unchanged (3:3:1) and the amount is increased to a total of 8 parts, and all other conditions are the same.

[0086] The specific formulations of the compositions in Examples 1-4 and Comparative Examples 1-6 are shown in Table 1.

[0087] Table 1. Specific formulations of the compositions in Examples 1-4 and Comparative Examples 1-6

[0088]

[0089] The applicant conducted antioxidant performance tests on the compositions provided in Examples 1-4 and Comparative Examples 1-6.

[0090] Application Example 1

[0091] The specific test method for determining DPPH free radical scavenging activity is as follows:

[0092] 1) Solution preparation

[0093] Weigh 20 mg of DPPH, dissolve it in 95% ethanol, and dilute to 250 mL to prepare a 0.2 mmol / L DPPH solution for later use; prepare composition samples (solvent: 75% ethanol aqueous solution) of concentrations of 0.5 mg / mL, 1.0 mg / mL, 1.5 mg / mL, 2.0 mg / mL, and 2.5 mg / mL for the examples and comparative examples, respectively; and prepare solutions of Vc (water-soluble antioxidant) and BHT (lipid-soluble synthetic antioxidant) with the same concentration gradient.

[0094] 2) DPPH reaction system

[0095] The reaction was carried out using a 96-well plate, with 200 μL of the test system added to each sample well. The groups were set as follows:

[0096] Experimental group: Equal volumes of the composition samples from each example and comparative example and 0.2 mmol / L DPPH solution were mixed in the sample wells; Background control group: Equal volumes of the composition samples from each example and comparative example and 75% ethanol aqueous solution were mixed in the sample wells; Blank group: Equal volumes of 75% ethanol aqueous solution and 0.2 mmol / L DPPH solution were mixed in the sample wells; Positive control group: Equal volumes of Vc and 0.2 mmol / L DPPH solution were mixed in the sample wells; Equal volumes of BHT solution and 0.2 mmol / L DPPH solution were mixed in the sample wells.

[0097] After mixing the test system in the above sample wells evenly, react at room temperature (25℃) in the dark for 45 min, and then use a spectrophotometer to measure the absorbance value at 517 nm.

[0098] 3) Calculate the free radical scavenging rate

[0099] Free radical scavenging rate = [1-(A1-A2) / A0] ×100%, where A1 is the absorbance value of the experimental group, A2 is the absorbance value of the sample background control group, and A0 is the absorbance value of the blank group.

[0100] The specific test results are shown in Table 2.

[0101] Table 2. Scavenging rate of DPPH free radicals by different compositions

[0102]

[0103] As shown in Table 2, the compositions provided in Examples 1-4 maintained a relatively stable DPPH radical scavenging rate of 69.14-78.16% at 2.5 mg / ml. In contrast, the compositions in Comparative Examples 1-6 only achieved a DPPH radical scavenging rate of 49.23-61.12% at 2.5 mg / ml, which is significantly lower than the DPPH radical scavenging rate of the compositions in this application.

[0104] Furthermore, as demonstrated in Examples 1 and 2, the bird's nest peptide works synergistically with other raw material components in this application, which is beneficial to improving the DPPH free radical scavenging rate of the composition. With the same total amount of raw material components, the addition of bird's nest peptide significantly improves the DPPH free radical scavenging rate of the composition, thus contributing to the synergistic antioxidant effect of the composition.

[0105] As can be seen from Examples 2 and Comparative Examples 1-2, the synergistic effect of osmanthus powder and glutathione-enriched yeast extract in the technical solution of this application is beneficial to improving the DPPH free radical scavenging rate of the composition. When the osmanthus powder is replaced with an equal amount of glutathione-enriched yeast extract (Comparative Example 1), its DPPH free radical scavenging rate is significantly reduced compared with Example 2; when the glutathione-enriched yeast extract is replaced with an equal amount of osmanthus powder (Comparative Example 2), its DPPH free radical scavenging rate is significantly reduced compared with Example 2.

[0106] As shown in Examples 2 and Comparative Examples 3-6, the burdock root extract, cordyceps militaris extract, frost-dried white matsutake mushroom powder, and porcini mushroom concentrate in the technical solution of this application work together to improve the DPPH free radical scavenging rate of the composition, exhibiting a synergistic effect. Compared with Example 2, while maintaining the same total amount of burdock root extract, cordyceps militaris extract, frost-dried white matsutake mushroom powder, and porcini mushroom concentrate, the compositions provided in Comparative Examples 3-6 show a significantly reduced DPPH free radical scavenging rate regardless of whether the burdock root extract, cordyceps militaris extract, frost-dried white matsutake mushroom powder, or porcini mushroom concentrate is removed.

[0107] Application Example 2

[0108] The ABTS free radical scavenging activity assay, specifically the test method is as follows:

[0109] 1) Solution preparation

[0110] Prepare a 7 mmol / L ABTS aqueous solution and mix it with an equal volume of a 2.45 mmol / L potassium persulfate solution. Incubate at room temperature, avoiding contact with air.

[0111] After being exposed to light for 16 hours, ABTS free radicals are generated. Before use, the solution is diluted with PBS buffer until the absorbance is within the range of 0.7 ± 0.02 when measured at 734 nm using a spectrophotometer. This solution is then used as the working solution for subsequent experiments.

[0112] Composition samples (solvent: PBS buffer) of the examples and comparative examples were prepared at concentrations of 0.5 mg / ml, 1.0 mg / ml, 1.5 mg / ml, 2.0 mg / ml, and 2.5 mg / ml, respectively, along with Vc (water-soluble antioxidant) at the corresponding concentration gradients.

[0113] 2) ABTS reaction system

[0114] The reaction was carried out using a 96-well plate, with 200 μL of the test system added to each sample well. The groups were set as follows:

[0115] Experimental group: 50 μL of the composition sample from each example and comparative example was mixed with 150 μL of ABTS working solution in each sample well; Background control group: 50 μL of the composition sample from each example and comparative example was mixed with 150 μL of PBS buffer in each sample well; Blank group: 50 μL of PBS buffer and 150 μL of ABTS working solution were mixed in each sample well; Positive control group: 50 μL of Vc and 150 μL of ABTS working solution were mixed in each sample well.

[0116] After mixing the test system in the above sample wells evenly, react at room temperature (25℃) in the dark for 30 min, and then use a spectrophotometer to measure the absorbance value at 734 nm.

[0117] 3) Calculate the free radical scavenging rate

[0118] Free radical scavenging rate = [1-(A1-A2) / A0] ×100%, where A1 is the absorbance value of the experimental group, A2 is the absorbance value of the sample background control group, and A0 is the absorbance value of the blank group.

[0119] The specific test results are shown in Table 3.

[0120] Table 3. Scavenging rates of different compositions against ABTS free radicals

[0121]

[0122] As shown in Table 3, the compositions provided in Examples 1-4 exhibit an ABTS radical scavenging rate of 66.52-82.18% at 2.5 mg / ml. In contrast, the compositions in Comparative Examples 1-6 show an ABTS radical scavenging rate of only 45.98-58.36% at 2.5 mg / ml, which is significantly lower than the ABTS radical scavenging rate of the compositions in this application.

[0123] Furthermore, as demonstrated in Examples 1 and 2, the bird's nest peptide works synergistically with other raw material components in this application, which is beneficial to improving the composition's ABTS free radical scavenging rate. With the same total amount of raw material components, the addition of bird's nest peptide significantly improves the composition's ABTS free radical scavenging rate, thus contributing to the composition's synergistic antioxidant effect.

[0124] As can be seen from Examples 2 and Comparative Examples 1-2, the synergistic effect of osmanthus powder and glutathione-enriched yeast extract in the technical solution of this application is beneficial to improving the ABTS free radical scavenging rate of the composition. When the osmanthus powder is replaced with an equal amount of glutathione-enriched yeast extract (Comparative Example 1), its ABTS free radical scavenging rate is significantly reduced compared with Example 2; when the glutathione-enriched yeast extract is replaced with an equal amount of osmanthus powder (Comparative Example 2), its ABTS free radical scavenging rate is significantly reduced compared with Example 2.

[0125] As shown in Examples 2 and Comparative Examples 3-6, the burdock root extract, cordyceps militaris extract, frost-dried white matsutake mushroom powder, and porcini mushroom concentrate in the technical solution of this application, when used in combination, collectively improve the ABTS free radical scavenging rate of the composition, exhibiting a synergistic effect. Compared with Example 2, while maintaining the same total amount of burdock root extract, cordyceps militaris extract, frost-dried white matsutake mushroom powder, and porcini mushroom concentrate, the ABTS free radical scavenging rate of the compositions provided in Comparative Examples 3-6 is reduced regardless of whether the burdock root extract, cordyceps militaris extract, frost-dried white matsutake mushroom powder, or porcini mushroom concentrate is removed.

[0126] In summary, the composition with antioxidant and whitening effects provided by this application has a good scavenging effect on ABTS and DPPH free radicals, and can clear different types of free radicals, thereby achieving antioxidant function.

[0127] Application Example 3

[0128] The applicant also used a zebrafish model to determine the antioxidant properties of the compositions in the various embodiments and comparative examples.

[0129] The experimental materials were: wild-type AB strain zebrafish embryos (purchased from Huante Biotechnology), phenylthiourea (purchased from Shanghai Yuanye Biotechnology Co., Ltd., S60243), methylcellulose (sigma, CAS: 497-76-7), and reactive oxygen species detection kit (Beyotime Biotechnology Co., Ltd., S0033M).

[0130] 1) Maximum Tolerable Concentration (MTC) Test of Samples in Zebrafish

[0131] Normally developing zebrafish embryos, 6 days post-fertilization (6 dpf), were randomly selected and placed into six-well culture plates, 15 embryos per well. 3 mL of different treatment groups were added to each well. The control group consisted of only standard dilution water; the experimental group consisted of solutions containing different concentrations of the compositions provided in Examples 1-4 and Comparative Examples 1-6 (prepared with standard dilution water); and the model control group consisted of 0.0445 mg / L menadione (menadione is a strong oxidative stressor that induces a large amount of free radicals in zebrafish). The culture plates were covered with the front panel and wrapped with aluminum foil, and incubated in a biochemical incubator at (28.5±1)℃ in the dark for 24 hours. The MTC of the samples on zebrafish was then measured. The test results are shown in Tables 4.1-4.2.

[0132] Table 4.1 Maximum Tolerable Concentration Test of the Compositions in the Examples for Zebrafish

[0133]

[0134] Table 4.2 Maximum Tolerable Concentration Tests of the Compositions in the Comparative Examples on Zebrafish

[0135]

[0136] As shown in Tables 4.1-4.2, the optimal MTC for the zebrafish used in this experiment was 0.5 mg / mL.

[0137] 2) Experiment on the effect of the test sample on the reactive oxygen species content in zebrafish

[0138] a) Grouping according to the following method:

[0139] Blank control group: containing zebrafish embryos and standard diluted water containing phenylthiourea. Only one normal control group needs to be set up for each experiment.

[0140] Model group control group: containing zebrafish embryos and 0.0445 mg / L menaquinone solution;

[0141] Experimental group: zebrafish embryos, 0.0445 mg / L menadione solution and 0.5 mg / mL of the composition sample solutions provided in Examples 1-4 and Comparative Examples 1-6 (solvent is standard dilution water).

[0142] b) Observation

[0143] Zebrafish juveniles were cultured for 24 hours using the different groups described above. They were then stained at 28°C with the DCFH-DA fluorescent ROS probe from a 10 μM reactive oxygen species detection kit (Beyotime Biotechnology Co., Ltd., S0033M) for 30 minutes in the dark. After staining, they were washed with standard dilution water for 2–3 hours.

[0144] Ten zebrafish were randomly selected and fixed with 3% methylcellulose. Fluorescence images of the juvenile zebrafish were observed and photographed under a fluorescence microscope (stronger fluorescence indicates higher intracellular ROS levels). ImageJ software was used to analyze and collect data, and the relative content of reactive oxygen species in the zebrafish bodies was analyzed. The statistical analysis results of this index were used to evaluate the sample's ability to inhibit menadione-induced reactive oxygen species generation. Statistical results are expressed as mean ± SE. Graphpad Prism software was used for plotting and statistical analysis; p < 0.05 indicated statistical significance. The test results are shown in Table 5.

[0145] The formula for calculating ROS clearance rate is: C = (S0 - S1) / S0 × 100%, where C is the ROS clearance rate (%), S0 is the average ROS fluorescence signal intensity of the model control group, and S1 is the average ROS fluorescence signal intensity of the experimental group.

[0146] Table 5. Effects of different compositions on reactive oxygen species content in zebrafish.

[0147]

[0148] Note: Compared with the model group, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001

[0149] Table 5 shows that under the experimental conditions, the green fluorescence in zebrafish in the blank control group was weak, indicating a low content of reactive oxygen species (ROS). In contrast, the green fluorescence in zebrafish in the model control group was strong, indicating that the ROS content increased after menadione induction. Compared to the model control group, treatment with the compositions in Examples 1-4 significantly reduced the green fluorescence in zebrafish, with ROS scavenging rates ranging from 49.7% to 58.2%. This demonstrates that the compositions provided in this application have the effect of scavenging some of the ROS induced by menadione.

[0150] However, the ROS removal rate after treatment with the composition in the comparative example was significantly lower than that of the composition in the embodiments of this application. This indicates that only by using the composition in this application can the technical effect of this application be achieved.

[0151] Application Example 4

[0152] The applicant also used a zebrafish model to determine the whitening effect of the compositions in the various embodiments and comparative examples.

[0153] The experimental materials were: wild-type AB strain zebrafish embryos (purchased from Huante Biotechnology), methylcellulose (Sigma, CAS: 497-76-7), and β-arbutin (Shanghai Yuanye Biotechnology Co., Ltd.).

[0154] 1) Maximum Tolerable Concentration (MTC) Test of Samples in Zebrafish

[0155] Zebrafish embryos with normal development, 3 days post-fertilization (3 dpf), were randomly selected and placed into six-well culture plates, 30 embryos per well. 3 mL of different treatment groups were added to each well. The control group consisted only of standard dilution water; the experimental group consisted of solutions containing different concentrations of the compositions provided in Examples 1-4 and Comparative Examples 1-6 (prepared with standard dilution water); and the positive control group consisted of β-arbutin at 4.5 mg / mL (β-arbutin is a tyrosinase inhibitor, and tyrosinase is a key enzyme in melanin synthesis; therefore, β-arbutin can competitively inhibit the activity of this enzyme, reducing melanin production at its source). The culture plates were covered with the plate panel and wrapped with aluminum foil, and incubated in a biochemical incubator at (28.5±1)℃ in the dark for 2 days. The MTC of the samples in zebrafish was then measured. The test results are shown in Tables 6.1-6.2.

[0156] Table 6.1 Maximum Tolerable Concentration Tests of the Compositions in the Examples for Zebrafish

[0157]

[0158] Table 6.2 Maximum Tolerable Concentration Tests of the Compositions in the Comparative Examples for Zebrafish

[0159]

[0160] As shown in Tables 6.1-6.2, the optimal MTC for the zebrafish used in this experiment was 0.312 mg / mL.

[0161] 2) Experiment on the effect of the test sample on the melanin content in the head of zebrafish

[0162] a) Grouping according to the following method:

[0163] Blank control group: containing zebrafish embryos and standard dilution water; only one normal control group needs to be set up for each experiment.

[0164] Positive control group: Contains zebrafish embryos and β-arbutin, with a β-arbutin concentration of 4.5 mg / mL;

[0165] Experimental group: Sample solutions containing zebrafish embryos and the compositions provided in Examples 1-4 and Comparative Examples 1-6 at a concentration of 0.312 mg / mL (solvent was standard dilution water).

[0166] b) Observation

[0167] Zebrafish juveniles were cultured at 28℃ for 2 days using the different groups described above. Ten zebrafish were randomly selected, fixed with 3% methylcellulose, observed and photographed under a stereomicroscope, and the data were analyzed and collected using ImageJ advanced image processing software. The melanin signal intensity in the zebrafish head was analyzed, and the statistical analysis results of this index were used to evaluate the ability of the samples to inhibit melanin production. Statistical results are expressed as mean ± SE. Graphpad Prism software was used for plotting and statistical analysis. p < 0.05 indicated that the difference was statistically significant. Detailed results are shown in Table 7.

[0168] The formula for calculating the melanin inhibition rate is: C = (S0 - S1) / S0 × 100%, where C is the melanin inhibition rate of the test substance (%), S0 is the average melanin signal intensity of the blank control group (n = 10), and S1 is the average melanin signal intensity of the experimental group (n = 10).

[0169] Table 7. Effects of different compositions on melanin content in zebrafish heads.

[0170]

[0171] Note: Compared with the blank control group, *p<0.05, **p<0.01, ***p<0.001, ****p < 0.0001

[0172] As shown in Table 7, under the experimental conditions, compared with the blank control group, the compositions provided in Examples 1-4 of this application significantly reduced the intensity of melanin signal in the zebrafish head by 60.34-76.16%. This indicates that the compositions with antioxidant and whitening effects provided by the technical solution of this application can significantly inhibit melanin production and have a whitening effect.

[0173] As shown in Comparative Examples 1-6, when using the compositions provided in the comparative examples, although they can also reduce the intensity of melanin signals in the zebrafish head to some extent compared with the blank control group, the degree of reduction is not as good as that in Example 2 of this application. This indicates that only the compositions obtained using the technical solution provided in this application can have such a good effect on inhibiting melanin production.

[0174] The above embodiments are merely illustrative of the principles and effects of the present invention and are not intended to limit the invention. Any person skilled in the art can modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by those skilled in the art without departing from the spirit and technical concept disclosed in the present invention should still be covered by the claims of the present invention.

Claims

1. A composition having antioxidant and whitening effects, characterized in that, It contains at least the following raw material components by weight: 20-40 parts of glutathione-rich yeast extract, 0.5-5 parts of burdock root extract, 0.5-5 parts of cordyceps extract, 0.2-2 parts of freeze-dried white matsutake mushroom powder, 0.2-2 parts of concentrated porcini mushroom powder, and 20-40 parts of osmanthus powder; The raw materials of the composition also include bird's nest peptides, and the weight ratio of the glutathione-enriched yeast extract to the bird's nest peptides is 20~40:1~10.

2. The composition according to claim 1, characterized in that, The composition comprises the following raw material components in parts by weight: 25-35 parts of glutathione-rich yeast extract, 2-5 parts of burdock root extract, 2-4 parts of cordyceps extract, 2-5 parts of bird's nest peptide, 0.5-1.5 parts of freeze-dried white matsutake mushroom powder, 0.5-1.5 parts of concentrated porcini mushroom powder, and 25-35 parts of osmanthus powder.

3. The composition according to claim 1, characterized in that, The glutathione-enriched yeast extract contains 15-25 wt% glutathione. And / or, the sialic acid content in the bird's nest peptide is 1~5 wt%; And / or, the polysaccharide content in the burdock root extract is 25-35 wt%; And / or, the polysaccharide content in the Cordyceps militaris extract is 25-35 wt%; And / or, the content of matsutake polysaccharides in the freeze-dried white matsutake powder is 25-35 wt%; And / or, the ergothioneine content in the porcini mushroom concentrate is 0.5-3 wt%; And / or, the composition has an ABTS radical scavenging rate of 60-90% and a DPPH radical scavenging rate of 60-85%.

4. A method for preparing a composition with antioxidant and whitening effects as described in any one of claims 1 to 3, characterized in that, Includes the following steps: The composition is obtained by uniformly mixing the glutathione-enriched yeast extract, burdock root extract, cordyceps extract, white matsutake freeze-dried powder, porcini concentrated powder, and osmanthus powder; bird's nest peptide is also added during mixing.

5. The preparation method according to claim 4, characterized in that, After mixing, the mixture is dried at a temperature of 30-50°C.

6. The use of a composition having antioxidant and whitening effects as described in any one of claims 1 to 3 in the preparation of antioxidant products.

7. A product with antioxidant properties, characterized in that, It contains the composition with antioxidant and whitening effects as described in any one of claims 1 to 3 and food-grade acceptable excipients.

8. The product according to claim 7, characterized in that, The excipients are selected from one or more of the following: sweeteners, acid regulators, fillers, colorants, antioxidants, thickeners, stabilizers, emulsifiers, anti-caking agents, flow aids, and lubricants. And / or, the product is selected from one or more of solid beverages, liquid beverages, dairy products, and flavoring powders.

9. The product according to claim 8, characterized in that, The excipients include maltodextrin; And / or, the product is a solid beverage.

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