Bio-enzyme deodorization and bacteriostasis spray and application thereof

Abstract

The invention discloses a bio-enzyme deodorization and bacteriostasis spray. Comprising the following components in parts by weight: 75 to 87 parts of deionized water, 1 to 3 parts of composite biological enzyme, 0.3 to 0.8 part of rosmarinic acid, 0.2 to 0.5 part of tea polyphenol, 0.5 to 1 part of honeysuckle flower extract, 0.1 to 0.3 part of dipotassium glycyrrhizinate, 0.05 to 0.1 part of low-sensitivity lavender essential oil, 0.3 to 0.6 part of polyglycerol-4 caprate, 0.2 to 0.4 part of polyoxyethylene sorbitan monooleate, 1 to 2 parts of glycerol and 0.8 to 1.5 parts of propylene glycol. The bio-enzyme deodorization and bacteriostasis spray is applied to odor dissolution. According to the bio-enzyme deodorization and bacteriostasis spray and the application, the peculiar smell of pets and the peculiar smell in the living environment of old people can be removed, meanwhile, the formula takes biodegradation peculiar smell and plant source bacteriostasis as the core, and the peculiar smell is effectively degraded through the synergistic effect of compound bio-enzyme and plant source activity; deodorization and bacteriostasis are achieved on peculiar smell of pets and the living environment of old people, plant sources are adopted as components in the formula, and secondary pollution to the environment is avoided.

Description

Technical Field

[0001] This invention belongs to the field of home deodorization and antibacterial, and relates to a bio-enzyme deodorizing and antibacterial spray and its application. Background Technology

[0002] There are countless deodorizing sprays on the market, but their effectiveness and performance are often unsatisfactory.

[0003] Current pet deodorizing spray technologies mainly focus on bio-enzyme decomposition, plant extracts, hypoallergenic formulas, and long-lasting antibacterial effects. Many products use compound bio-enzymes and plant amino acids, which can efficiently decompose ammonia and hydrogen sulfide, and are advertised as suitable for sensitive pets; however, in reality, the sensitization rate is very high. In addition, nano-slow-release technology and microencapsulation technology have also been used to prolong the deodorizing effect, but these products suffer from insufficient durability of effect, requiring frequent use, and therefore cannot achieve satisfactory results.

[0004] For deodorizing sprays used by the elderly, existing products mostly rely on fragrance to mask the odor rather than actually breaking it down, resulting in poor effectiveness and potential discomfort. Many products are merely air fresheners, failing to effectively remove the smell of urine, and may even make it smell worse when mixed with other products. Furthermore, skin irritation and safety are also pain points for products targeting the elderly, especially in the area of ​​incontinence care.

[0005] Therefore, existing technologies have the following shortcomings: the stability of biological enzymes, adaptability to complex odors, and long-term effects on pet skin. Deodorizing sprays for the elderly, on the other hand, need to address issues such as incomplete odor decomposition, strong ingredient irritation, and insufficient long-lasting effects.

[0006] From a technical perspective, the issues can be summarized as follows: 1. It has insufficient adaptability to complex odors and low decomposition efficiency for vomit and volatile fatty acids produced by oil oxidation, such as butyric acid, requiring the use of physical cleaning methods.

[0007] Second: Performance limitations in extreme environments. Enzyme activity may decrease at high temperatures, and frequent respraying is required in high humidity environments. Low temperature tests show that some products are stable at -5℃, but in actual applications, they may still be affected by the material.

[0008] Third: Controversy over long-lasting effect and residue. Some products claim "72-hour antibacterial effect", but users report that the odor may rebound after 24 hours, and the spray residue may damage the cilia of the pet's respiratory tract.

[0009] Fourth: Ingredient compatibility challenges. Plant essential oils such as tea tree oil are toxic to cats and their concentrations must be strictly controlled. Bioenzymes and bactericides may have antagonistic effects.

[0010] Therefore, there is an urgent need for a deodorizing spray that can be used effectively for pet odors, as well as for the elderly, with safe ingredients and effective results. Summary of the Invention

[0011] The purpose of this invention is to overcome the shortcomings of existing technologies by proposing a bio-enzyme deodorizing and antibacterial spray and its application, which can eliminate pet odors and odors in the living environment of the elderly. At the same time, the formula is based on biodegradation of odors and plant-derived antibacterial agents. Through the synergistic effect of compound bio-enzymes and plant-derived active agents, it achieves deodorization and antibacterial effects on pet odors and the living environment of the elderly. Moreover, the formula uses plant-derived ingredients, so there is no secondary environmental pollution.

[0012] To achieve the above objectives, the present invention adopts the following technical solution: a bio-enzyme deodorizing and antibacterial spray, comprising, by weight percentage: 75-87 parts deionized water 1-3 parts of compound bio-enzyme 0.3-0.8 parts rosmarinic acid Tea polyphenols 0.2-0.5 parts Honeysuckle extract 0.5-1 part Dipotassium glycyrrhizate 0.1-0.3 parts Hypoallergenic lavender essential oil 0.05-0.1 parts Polyglycerol-4-decanoate 0.3-0.6 parts 0.2-0.4 parts of polyoxyethylene dehydrated sorbitan monooleate 1-2 parts glycerin And 0.8-1.5 parts of propylene glycol.

[0013] As a further description of the above technical solution: A bio-enzyme deodorizing and antibacterial spray, calculated by mass percentage, comprises: 82 parts of deionized water 2 portions of compound bio-enzyme 0.5 parts rosmarinic acid 0.3 parts tea polyphenols Honeysuckle extract 0.7 parts Dipotassium glycyrrhizate 0.2 parts Hypoallergenic lavender essential oil 0.08 parts 0.4 parts of polyglycerol-4-decanoate 0.3 parts of polyoxyethylene dehydrated sorbitan monooleate 1.5 parts glycerin 1.2 parts of propylene glycol.

[0014] As a further description of the above technical solution: A bio-enzyme deodorizing and antibacterial spray, calculated by mass percentage, comprises: 82 parts of deionized water Two portions of protease, lipase, and amylase 0.5 parts rosmarinic acid 0.3 parts tea polyphenols Honeysuckle extract 0.7 parts Hypoallergenic lavender essential oil 0.08 parts 0.3 parts of polyoxyethylene dehydrated sorbitan monooleate Citric acid 0.15 parts Sodium citrate 0.15 parts 1.5 parts glycerin 1.2 parts of propylene glycol.

[0015] As a further description of the above technical solution: a bio-enzyme deodorizing and antibacterial spray, calculated by mass percentage, includes 86 portions of deionized water One part papain and neutral lipase Honeysuckle extract 0.8 parts Rosemary extract 1.1 parts Dipotassium glycyrrhizate 0.2 parts Desensitizing chamomile essential oil 0.01 parts 0.4 parts of polyglycerol-4-decanoate 0.2 parts of phenoxyethanol Sodium dihydrogen phosphate 0.2 parts 0.3 parts of disodium hydrogen phosphate 5 parts glycerin 3 parts isopropanol.

[0016] As a further description of the above technical solution: the composite bioenzyme is a protease, lipase, amylase and / or cellulase.

[0017] As a further description of the above technical solution: the hypoallergenic lavender essential oil is distilled hypoallergenic lavender essential oil.

[0018] As a further description of the above technical solution: the tea polyphenols are tea polyphenols extracted from green tea.

[0019] As a further description of the above technical solution: the honeysuckle extract is obtained by water extraction.

[0020] An application of a bio-enzyme deodorizing and antibacterial spray, comprising the bio-enzyme deodorizing and antibacterial spray, wherein the bio-enzyme deodorizing and antibacterial spray is applied to odor dissolution.

[0021] Due to the adoption of the above technical solution, the beneficial effects achieved by this invention are: (1) The present invention can decompose 90% of pet urine and feces and odors in shoe-related rooms within 2 hours, and there is no rebound within 48 hours. At the same time, the enzymatic hydrolysis products can be naturally degraded without environmental residues, and the deodorization is thorough and long-lasting. (2) The bio-enzyme deodorizing and antibacterial spray designed by the present invention can achieve a bacteriostatic rate of ≥98% for common pathogens, is safe for pets to lick (LD50>5000mg / kg), and has an inhalation and skin contact irritation rate of <1% for the elderly, making it safe and non-irritating for antibacterial purposes. (3) This invention targets the sensitive respiratory tracts of the elderly and the thin skin of pets, reducing the adverse reaction rate from 20% to less than 1%, solving the problem that existing sprays are effective but cause allergies, and has strong anti-allergy compatibility; (4) Through the design of the nozzle, the present invention can achieve a spray particle diameter of 10-20μm, which can be suspended for 15 minutes. The coverage area can be increased by 60% compared with the existing spray. There is no dripping phenomenon during use, resulting in a good user experience, fine atomization and uniform coverage. (5) All ingredients of this invention are derived from plant extracts and bio-fermentation, are biodegradable, and have no secondary pollution from chemical additives, which is in line with the trend of "green home". Detailed Implementation

[0022] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only a part of the embodiments of the present invention, and not all of them. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0023] The present invention will be described in detail with reference to the following embodiments: Example 1

[0024] This embodiment provides a bio-enzyme deodorizing and antibacterial spray, which, by weight percentage, comprises: 75-87 parts deionized water 1-3 parts of compound bio-enzyme 0.3-0.8 parts rosmarinic acid Tea polyphenols 0.2-0.5 parts Honeysuckle extract 0.5-1 part Dipotassium glycyrrhizate 0.1-0.3 parts Hypoallergenic lavender essential oil 0.05-0.1 parts Polyglycerol-4-decanoate 0.3-0.6 parts 0.2-0.4 parts of polyoxyethylene dehydrated sorbitan monooleate 1-2 parts glycerin Propylene glycol 0.8-1.5 parts.

[0025] Because the odors in pet environments often contain protein, oil, and carbohydrates, and because the elderly have weaker immune systems, their living environments often pose risks of respiratory and skin irritation.

[0026] In this embodiment, the base solvent is deionized water. Using deionized water ensures the effective concentration of each component and avoids excessive concentration that could irritate the body. The core deodorizing component is a compound biological enzyme with an enzyme activity of 100,000 U / g, which can decompose 90% of pet urine and feces odor within 2 hours.

[0027] In plant-derived antibacterial agents, we select three components to form a compound antibacterial agent. The specific functions and mechanisms of action are as follows: (1) The added ingredient rosmarinic acid has an antibacterial rate of ≥99% against Escherichia coli / Staphylococcus aureus and has been tested on rabbit skin and has no skin irritation. (2) Add tea polyphenols, because tea polyphenols can work synergistically with rosmarinic acid to increase the antifungal rate to 98%; (3) Honeysuckle extract can reduce the mild irritation of rosmarinic acid to the mucous membrane and is suitable for the respiratory tract of the elderly.

[0028] The preferred anti-allergy agent is dipotassium glycyrrhizate. This anti-allergy agent has been tested on human patches and can reduce the risk of redness in sensitive skin by 80%, making it very suitable for people who frequently come into contact with pets and for the living environment of the elderly.

[0029] The plant-derived fragrance is made with hypoallergenic lavender essential oil, has a mild scent, and has been shown to exhibit no avoidance behavior in pets through behavioral observation experiments.

[0030] The solubilizing emulsifier is polyglycerol-4-decanoate, which keeps the spray particle diameter stable at 10-20μm, resulting in fine atomization without dripping.

[0031] Polyoxyethylene dehydrated sorbitan monooleate ensures that the complex bio-enzymes and antibacterial components do not separate into layers after 6 months of accelerated testing at 45°C.

[0032] Glycerin and propylene glycol were selected as moisturizing stabilizers to maintain enzyme activity. Tests showed that the enzyme activity retention rate was ≥85% after 6 months. This addition can prevent dryness of the elderly's skin after contact.

[0033] Propylene glycol is fermented from corn and works synergistically with glycerol to improve the low-temperature stability of the formula, ensuring that the product does not freeze at -5°C.

[0034] Therefore, the formulation design of Example 1 can effectively eliminate pet odors and odors in the living environment of the elderly. The core of the formulation design is biodegradable odor and plant-derived antibacterial. The deodorization design for pet odors and the living environment of the elderly avoids problems such as chemical masking deodorization, single antibacterial ingredients and pollution from chemical additives. Through the synergy of compound biological enzyme system and plant-derived active ingredients, the functions of deodorization and antibacterial are achieved. Moreover, the formulation design follows the design of plant-derived ingredients, avoiding secondary pollution to the environment. Example 2

[0035] This embodiment provides an optimized formula for a bio-enzyme deodorizing and antibacterial spray, which, calculated by weight percentage, includes: 82 parts of deionized water 2 portions of compound bio-enzyme 0.5 parts rosmarinic acid 0.3 parts tea polyphenols Honeysuckle extract 0.7 parts Dipotassium glycyrrhizate 0.2 parts Hypoallergenic lavender essential oil 0.08 parts 0.4 parts of polyglycerol-4-decanoate 0.3 parts of polyoxyethylene dehydrated sorbitan monooleate 1.5 parts glycerin 1.2 parts of propylene glycol.

[0036] Current technologies often use single enzymes, such as proteases, and chemical fragrances for deodorization. However, the addition of a single enzyme can only break down protein-based odors, such as pet urine, but cannot mask oily odors. For example, odors caused by the decomposition of pet food residue, pet hair, or dander, which produce carbohydrate-based odors, are easily masked by fragrances and tend to reappear.

[0037] Therefore, in this embodiment, we adopt the design concept of a composite bio-enzyme, and we have selected protease, lipase, amylase, and cellulase as the composite bio-enzymes. Among them, protease can decompose urea and protein, thus removing ammonia and amine odors; lipase can decompose oils in pet food, thus removing fatty acid odors; amylase can decompose carbohydrates, such as pet treat residue, thus removing organic acid odors; and cellulase can decompose fibers in the environment, such as carpet fibers, thus removing humus odors.

[0038] In practical applications, maintaining enzyme activity is an unavoidable issue. Improving the stability of bio-enzyme deodorizing and antibacterial sprays hinges on addressing the problem that bio-enzymes, such as protein-based active substances, are easily deactivated by environmental factors. These environmental factors, such as temperature, pH, oxidation, metal ions, and microorganisms, are unavoidable in daily life, thus placing numerous limitations on formula design.

[0039] This formula, through the addition of multiple compound bio-enzymes, enables the enzymatic decomposition of odor molecules to replace chemical masking, eliminating odors at their source. The enzymatic decomposition products are water, carbon dioxide, and small-molecule organic matter. Furthermore, the generated substances are biodegradable, causing no secondary pollution.

[0040] In terms of bactericides, existing technologies mostly use chemical bactericides, such as benzalkonium chloride and chlorhexidine. Although they have strong antibacterial effects, they can irritate the mucous membranes, eyes, and noses of pets and the sensitive respiratory tracts of the elderly. Furthermore, their residues can easily cause secondary environmental pollution and inhibit soil microorganisms.

[0041] The formulation in this embodiment selects three plant-derived ingredients: 1. Rosmarinic acid: Broad-spectrum antibacterial, capable of inhibiting bacteria and fungi. At the same time, it has good thermal stability, retaining 80% of its activity after sterilization at 121℃. This choice avoids loss of activity during high-temperature storage. II. Tea polyphenols: assist in antibacterial and antioxidant effects, and reduce the production of new odors by oxidizing odor precursors such as unsaturated fatty acids; 3. Honeysuckle extract: A traditional, mild antibacterial ingredient that reduces the irritation of rosmarinic acid to sensitive individuals or pets, achieving a balance between highly effective antibacterial activity and low irritation.

[0042] Acute oral toxicity testing (LD50 > 5000 mg / kg) showed that the product remains safe even if pets accidentally lick it; at the same time, the respiratory irritation rate in the elderly was <1%, while the irritation rate of existing chemical antibacterial sprays was >15%. Therefore, in this embodiment, our formulation effectively reduces the irritation risk of chemical antibacterial agents.

[0043] This embodiment overcomes the limitations of single-enzyme deodorization, covering over 95% of odor types in pet scenarios. After enzymatic hydrolysis, the odor residue rate is <5%, compared to existing masking sprays with a rebound rate >50% after 24 hours. Our product solves the problem of odor rebound. It also exhibits an antibacterial rate of ≥98% against common pet pathogens such as Escherichia coli, Staphylococcus aureus, and ringworm fungi, breaking through the limitations of existing single-plant antibacterial ingredients that are only effective against bacteria. Example 3

[0044] In the specific environment of pet use, we have adjusted certain added ingredients in the formulation of this embodiment to better suit pet-friendly environments. Specifically, a bio-enzyme deodorizing and antibacterial spray, calculated by weight percentage, includes: 82 parts of deionized water Two portions of protease, lipase, and amylase 0.5 parts rosmarinic acid 0.3 parts tea polyphenols Honeysuckle extract 0.7 parts Hypoallergenic lavender essential oil 0.08 parts 0.3 parts of polyoxyethylene dehydrated sorbitan monooleate Citric acid 0.15 parts Sodium citrate 0.15 parts 1.5 parts glycerin 1.2 parts of propylene glycol.

[0045] Because pet environments often have unpleasant odors, with high concentrations of urine, feces, and grease, these require rapid decomposition by highly active enzymes. In this embodiment, we preferentially use protease, lipase, and amylase, with each enzyme comprising 2% of the total content. Our formulation uses a combination of these three enzymes: protease, lipase, and amylase. The interaction between these enzymes can decompose 90% of the odor from urine and feces within 2 hours without irritating the pet's mucous membranes.

[0046] Regarding the selection of solvents, we chose 82 parts deionized water and 1.2 parts propylene glycol. Propylene glycol is necessary to enhance enzyme stability in pet environments, and the design must be specifically tailored to prevent accidental licking by pets, ensuring an enzyme activity retention rate of ≥85% after 6 months. Propylene glycol is derived from corn fermentation, making it safe for pets to lick, and it also has other functional properties.

[0047] Because pets require broad-spectrum antibacterial agents that cover E. coli and ringworm fungi, we selected 0.5 parts rosmarinic acid, 0.3 parts tea polyphenols, and 0.7 parts honeysuckle extract, totaling 1.5 parts, accounting for 1.5% of the overall formula content. Rosmarinic acid and tea polyphenols synergistically enhance the antifungal effect, achieving an inhibition rate of ≥98% against ringworm fungi in cats, and preventing skin redness in pets.

[0048] Because pets have a sensitive sense of smell, lavender essential oil can reduce their avoidance behavior towards sprays. In this embodiment, 0.08 parts of distilled, hypoallergenic lavender essential oil were used. During use, the pet did not avoid the area where the spray was applied, and a faint fragrance remained in the environment.

[0049] Regarding preservatives and emulsifiers, in this embodiment, we selected plant-derived polyoxyethylene sorbitan monooleate (POD), with 0.3 parts of POD, which enhanced the emulsification and preservative effects. During testing, we found that the deodorizing spray with added POD maintained its properties for 6 months without stratification or microbial contamination.

[0050] Meanwhile, since the optimal pH for pet-grade compound enzymes is 6.0-6.5, we added 0.15 parts citric acid and 0.15 parts sodium citrate to this formula as a pH buffer to maintain the pH at 6.0-6.5. The effective addition of citric acid and sodium citrate in the formula ensures stable enzyme activity and prevents skin irritation in pets.

[0051] In pet environments, glycerin is needed to maintain enzyme activity and prevent enzyme deactivation due to dryness. Therefore, we added 1.5 parts glycerin as a humectant. In specific tests, we found that the enzyme activity retention rate was ≥80% in a dry litter box environment.

[0052] The specific data is shown in Table 1 below.

[0053] .

[0054] Therefore, the formula in this embodiment is more suitable for use in pet environments. Example 4

[0055] For the use of elderly people, we provide another optimized formula in this embodiment, which can further meet the needs of elderly people in their living and working scenarios.

[0056] A bio-enzyme deodorizing and antibacterial spray, comprising, by weight percentage: 86 portions of deionized water One part papain and neutral lipase Honeysuckle extract 0.8 parts Rosemary extract 1.1 parts Dipotassium glycyrrhizate 0.2 parts Desensitizing chamomile essential oil 0.01 parts 0.4 parts of polyglycerol-4-decanoate 0.2 parts of phenoxyethanol Sodium dihydrogen phosphate 0.2 parts 0.3 parts of disodium hydrogen phosphate 5 parts glycerin 3 parts isopropanol.

[0057] Because the skin and respiratory tract of the elderly are sensitive, high enzyme activity may cause irritation. Plant-derived enzymes are gentler. Therefore, the compound enzymes in this formula are plant-derived compound enzymes, specifically papain and neutral lipase, in a total of 1 part, with an enzyme activity of 50,000 U / g. They can decompose food and sweat odors within 4 hours, and there is no tightness on the skin or coughing in the respiratory tract.

[0058] Because elderly people need glycerin to moisturize and prevent dry skin, and because isopropyl alcohol is volatile and can reduce furniture residue, and because some people may be intolerant to propylene glycol, isopropyl alcohol is used instead of propylene glycol in this embodiment. Therefore, the formula uses 86 parts deionized water, 5 parts glycerin, and 3 parts isopropyl alcohol to achieve a dry, residue-free finish within 15 minutes of spraying, and to eliminate any dryness upon skin contact.

[0059] The environment for the elderly is dominated by bacteria, especially E. coli and mold. Reducing the mild irritation caused by rosmarinic acid and increasing the proportion of natural ingredients can reduce mucosal irritation. With the addition of 0.8 parts honeysuckle extract and 0.6 parts rosemary extract, totaling 1.4 parts, the test results showed that the antibacterial rate against bathroom mold was ≥95% and the respiratory irritation rate was <0.5%.

[0060] Elderly people have low olfactory tolerance, and strong odors can easily induce dizziness and coughing. Unscented or very lightly scented chamomile essential oil is more suitable. We use unscented or 0.01 part desensitized chamomile essential oil, which does not burden the sense of smell and has no adverse reactions in elderly people with rhinitis and asthma.

[0061] Elderly people are prone to skin allergies. To reduce the amount of chemical preservatives such as polyoxyethylene sorbitan monooleate, natural preservatives are added to lower the risk of contact sensitization. In this example, 0.2 parts of low-concentration phenoxyethanol and 0.5 parts of rosemary extract are used as natural preservatives, bringing the total amount of preservatives to 0.7 parts. Skin patch tests showed an allergy rate of <1%, and the product did not deteriorate within the shelf life.

[0062] The skin pH of the elderly is 6.5-7.5. Neutral phosphate buffer is closer to the skin pH and avoids acid and alkali irritation such as skin redness. The solution contains 0.2 parts sodium dihydrogen phosphate and 0.3 parts disodium hydrogen phosphate. The pH was tested to be 7.0. The spray does not cause stinging when it comes into contact with the hands and face. Long-term use will not cause barrier damage.

[0063] The specific results are shown in Table 2 below.

[0064] .

[0065] Therefore, the formula in this embodiment is more suitable for use in the living environment of the elderly. Example 5

[0066] In practice, we have found that the size of the atomized particles in a spray directly affects respiratory irritation, specifically in the following two aspects: Particles that are too fine (<5μm) can easily penetrate deep into the alveoli; Particles that are too coarse (>50μm) are prone to dripping. Therefore, it is necessary to control the particles within the "settling range" to reduce suspended inhalation. In this embodiment, we abandoned the traditional design of the original nozzle with particles of 5-15μm and replaced it with a fan-shaped wide-angle nozzle, controlling the atomized particle diameter to 10-20μm. In the experiment, the 10-20μm particles quickly settled on object surfaces, such as furniture and floors, and hardly remained suspended in the air. Since the suspension time of 5-15μm spray particles in the traditional design is >5 minutes, while the suspension time after using the fan-shaped wide-angle nozzle is <30 seconds, this significantly reduces the amount inhaled by the elderly. Simultaneously, because the particle size does not affect the contact efficiency between the enzyme and the odor, this design ensures that the deodorizing effect of the formula is not compromised, better adapting to the formula in this invention and improving the deodorizing effect. Example 6

[0067] In this embodiment, we added low molecular weight sodium hyaluronate to increase the viscosity of the spray liquid, controlling the viscosity at 15-20 mPa·s. Adding the anti-settling agent sodium hyaluronate prevents the spray particles from becoming suspended due to excessive lightness. Simultaneously, it is a natural moisturizer, causing no irritation when in contact with skin or the respiratory tract, and it also enhances enzyme adhesion, extending the antibacterial effect on object surfaces to 72 hours.

[0068] During use, we set the content of low molecular weight sodium hyaluronate at 0.1-0.15%, which can effectively increase the viscosity of the spray liquid without affecting the functionality of the formula.

[0069] To verify that the adjusted formula could achieve the desired non-irritating and effective results, we conducted targeted testing. This targeted testing required the following: The discomfort rate is <1%, the odor removal rate is ≥90% in 24 hours, the antibacterial rate is ≥95% in 72 hours, the VOC content is <0.05mg / m³, and the heavy metals (lead and mercury) content is <0.001mg / kg.

[0070] During the test, we selected 50 elderly people aged 60-80 as the test subjects, including 20% ​​of them with asthma or rhinitis. They were asked to use the spray in a 15㎡ enclosed room for 7 consecutive days, and the incidence of discomfort such as coughing, sneezing, and chest tightness was recorded.

[0071] During the testing process: 1. Deodorizing and antibacterial effect test: Simulating an environment for the elderly, containing food residue, sweat odor, and E. coli / mold concentration of 10. 5 CFU / m³, tested for odor removal rate in 24 hours and antibacterial rate in 72 hours; Second: Component safety verification: VOCs content and heavy metals are detected by GC-MS to avoid hidden irritants.

[0072] The experimental results are shown in Table 3 below.

[0073] .

[0074] Through the above adjustments, respiratory irritation can be reduced from the source of ingredients, contact path, and usage scenario without reducing the deodorizing and antibacterial effect. This is suitable for the fragile respiratory physiology of the elderly, while also taking into account long-lasting effects, reducing the frequency of use, and lowering the risk of cumulative irritation. Example 7

[0075] This embodiment is still optimized for the elderly, because the respiratory tract of the elderly is sensitive to volatile organic compounds (VOCs) and drying solvents, such as isopropanol and propylene glycol, which can easily trigger coughing from trace amounts of volatilization.

[0076] In an optimized embodiment, we replaced the solvent with a low-volatility, highly biocompatible solvent, while reducing mucosal dryness and irritation through moisturizing. Therefore, in this optimized embodiment, isopropanol was removed from the original formulation, the solvent was adjusted to 90 parts deionized water, and 5 parts of 1,3-propanediol from plant fermentation was added, while other ingredients remained unchanged.

[0077] We found that by replacing the solvent in the original formula with a zero-VOC solvent, the VOC content of 1,3-propanediol is <0.1mg / m³, and its volatility is only 1 / 20 that of isopropanol, with almost no inhalation irritation; at the same time, its moisturizing properties can maintain the moisture of the respiratory mucosa and reduce discomfort caused by dryness.

[0078] In summary, the present invention has achieved the following beneficial effects in relation to Examples 1-7: (1) The present invention can decompose 90% of pet urine and feces and odors in shoe-related rooms within 2 hours, and there is no rebound within 48 hours. At the same time, the enzymatic hydrolysis products can be naturally degraded without environmental residues, and the deodorization is thorough and long-lasting. (2) The bio-enzyme deodorizing and antibacterial spray designed by the present invention can achieve a bacteriostatic rate of ≥98% for common pathogens, is safe for pets to lick (LD50>5000mg / kg), and has an inhalation and skin contact irritation rate of <1% for the elderly, making it safe and non-irritating for antibacterial purposes. (3) This invention targets the sensitive respiratory tracts of the elderly and the thin skin of pets, reducing the adverse reaction rate from 20% to less than 1%, solving the problem that existing sprays are effective but cause allergies, and has strong anti-allergy compatibility; (4) Through the design of the nozzle, the present invention can achieve a spray particle diameter of 10-20μm, which can be suspended for 15 minutes. The coverage area can be increased by 60% compared with the existing spray. There is no dripping phenomenon during use, resulting in a good user experience, fine atomization and uniform coverage. (5) All ingredients of this invention are derived from plant extracts and bio-fermentation, are biodegradable, and have no secondary pollution from chemical additives, which is in line with the trend of "green home".

[0079] Finally, it should be noted that the above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A bio-enzyme deodorizing and bacteriostatic spray, characterized in that, By mass percent, comprising: Deionized water 75-87 parts Compound biological enzyme 1-3 parts Rosemary acid 0.3-0.8 parts Tea polyphenol 0.2-0.5 parts Honeysuckle extract 0.5-1 part Glycyrrhizin acid dipotassium 0.1-0.3 parts Low-sensitivity lavender essential oil 0.05-0.1 parts Polyglycerol-4 decanoate 0.3-0.6 parts Polyoxyethylene sorbitan monooleate 0.2-0.4 parts Glycerol 1-2 parts And propylene glycol 0.8-1.5 parts.

2. The bio-enzyme deodorizing and bacteriostatic spray according to claim 1, characterized in that, By mass percent, comprising: Deionized water 82 parts Compound biological enzyme 2 parts Rosemary acid 0.5 parts Tea polyphenol 0.3 parts Honeysuckle extract 0.7 parts Glycyrrhizin acid dipotassium 0.2 parts Low-sensitivity lavender essential oil 0.08 parts Polyglycerol-4 decanoate 0.4 parts Polyoxyethylene sorbitan monooleate 0.3 parts Glycerol 1.5 parts And propylene glycol 1.2 parts.

3. The bio-enzyme deodorizing and bacteriostatic spray according to claim 1, characterized in that, By mass percent, comprising: Deionized water 82 parts Protease, lipase and amylase 2 parts Rosemary acid 0.5 parts Tea polyphenol 0.3 parts Honeysuckle extract 0.7 parts Low-sensitivity lavender essential oil 0.08 parts Polyoxyethylene sorbitan monooleate 0.3 parts Citric acid 0.15 parts Sodium citrate 0.15 parts Glycerol 1.5 parts And propylene glycol 1.2 parts.

4. The bio-enzyme deodorizing and bacteriostatic spray according to claim 1, characterized in that, By mass percent, comprising Deionized water 86 parts Papain and neutral lipase 1 part Honeysuckle extract 0.8 parts Rosemary extract 1.1 parts Glycyrrhizin acid dipotassium 0.2 parts Desensitization chamomile essential oil 0.01 parts Polyglycerol-4 decanoate 0.4 parts Phenoxyethanol 0.2 parts Sodium dihydrogen phosphate 0.2 parts Disodium hydrogen phosphate 0.3 parts Glycerol 5 parts And isopropyl alcohol 3 parts.

5. The bio-enzyme deodorizing and bacteriostatic spray according to claim 1, characterized in that, The compound biological enzyme is protease, lipase, amylase and / or cellulase.

6. The bio-enzyme deodorizing and bacteriostatic spray according to claim 1, characterized in that, The low-sensitivity lavender essential oil is distillation grade low-sensitivity lavender essential oil.

7. The bio-enzyme deodorizing and bacteriostatic spray according to claim 1, characterized in that, The tea polyphenol is tea polyphenol extracted from green tea.

8. The bio-enzyme deodorizing and bacteriostatic spray according to claim 1, characterized in that, The honeysuckle extract adopts water extraction method.

9. The use of a bio-enzyme deodorizing and bacteriostatic spray, comprising the bio-enzyme deodorizing and bacteriostatic spray according to any one of claims 1-8, characterized in that, The biological enzyme deodorization bacteriostatic spray is applied to odor dissolution.