Cell culture reactor in full temperature control mode

Abstract

The invention relates to a cell culture reactor in a full temperature control mode, and relates to the technical field of cell culture reaction equipment. The cell culture reactor in the full temperature control mode comprises a shell, a heating cavity is formed in the shell, a bottom plate and a bottom heater are arranged in the heating cavity, the bottom plate is used for placing a cell reaction bag, the bottom heater is used for heating the bottom plate, a cavity heating mechanism is further arranged in the shell, and the cavity heating mechanism is used for heating the cell reaction bag. And the cavity heating mechanism comprises a heating fan, and the heating fan is used for heating gas in the heating cavity. The device has the effect of ensuring the heating uniformity of the cell reaction bag.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of cell culture reaction equipment, in particular to a cell culture reactor in full temperature control mode. BACKGROUND

[0002] The cell culture reactor is an analytical instrument used in the field of biological engineering, which can provide a suitable artificial environment for cell growth and reproduction, so as to carry out large-scale culture of cells in a controllable and sterile environment to produce the target product, and thus plays an important role in the fields of biopharmaceuticals, industrial biology and food industry.

[0003] There is a cell culture reactor in the prior art, which comprises a shell, a bottom plate and a bottom plate heating mechanism are arranged in the shell, the bottom plate is arranged in the shell and is used for placing a cell reaction bag. The bottom plate heating mechanism is used for heating the bottom plate, so that the bottom plate heats the cell reaction bag through heat transfer after being heated. In use, the cell reaction bag is placed on the bottom plate, and then the bottom plate heating mechanism is started to heat the cell reaction bag through heat transfer.

[0004] For the related technology in the above, since the bottom plate heating mode is adopted in the prior art to heat the cell reaction bag through heat transfer, only the bottom of the cell reaction bag is in direct contact with the bottom plate, which makes the heat always transferred from the bottom of the cell reaction bag to the rest of the cell reaction bag during the heating of the cell reaction bag by the bottom plate. This results in that the temperature of the bottom of the cell reaction bag is often higher than that of the rest of the cell reaction bag, thereby reducing the heating uniformity of the cell reaction bag and further reducing the heating effect of the cell reaction bag. Therefore, it needs to be improved. SUMMARY

[0005] In order to ensure the heating uniformity of the cell reaction bag, the present application provides a cell culture reactor in full temperature control mode.

[0006] The cell culture reactor in full temperature control mode provided by the present application adopts the following technical solution: A cell culture reactor in full temperature control mode, comprising a shell, a heating cavity is formed in the shell, a bottom plate and a bottom heater are arranged in the heating cavity, the bottom plate is used for placing a cell reaction bag, the bottom heater is used for heating the bottom plate, a chamber heating mechanism is further arranged in the shell, the chamber heating mechanism comprises a heating fan, and the heating fan is used for heating the gas in the heating cavity.

[0007] By adopting the technical scheme, compared with the prior art, only the bottom plate is heated, the bottom of the cell reaction bag is often higher than the rest, the heating uniformity of the cell reaction bag is reduced, and the heating effect of the cell reaction bag is reduced. The application is provided with a heating chamber heating mechanism, so that the heating fan can suck and heat the gas outside, and after heating, it is introduced into the heating chamber, so that the gas in the heating chamber can heat the rest of the surface of the cell reaction bag except the bottom by heat transfer, thereby increasing the heating uniformity of the cell reaction bag, and effectively ensuring the heating effect of the cell reaction bag.

[0008] As a preferred, the shell is further provided with a temperature control mechanism, the temperature control mechanism comprises a first temperature detection module, a second temperature detection module and a control module, the first temperature detection module, the second temperature detection module, the bottom heater and the heating fan are all in communication connection with the control module, the first temperature detection module is used for detecting the temperature of the bottom plate, the second temperature detection module is used for detecting the temperature in the heating chamber, and the control module is used for controlling the heating power of the bottom heater and the heating fan based on the temperatures detected by the first temperature detection module and the second temperature detection module.

[0009] By adopting the technical scheme, the first temperature detection module can detect the temperature of the bottom of the cell reaction bag, and the second temperature detection module can detect the temperature in the heating chamber, so that the control module can control the heating power of the bottom heater and the heating fan based on the detected temperature after receiving the temperatures of the first temperature detection module and the second detection module, thereby regulating and controlling the heating effect of the bottom heater and the heating fan, and effectively ensuring the heating effect of the cell reaction bag.

[0010] As a preferred, the control module further comprises a temperature comparison unit, the temperature comparison unit is used for comparing the temperature data detected by the first temperature detection module and the second temperature detection module, and when the temperature detected by the first temperature detection module is greater than the temperature detected by the second temperature detection module, the heating power of the heating fan is controlled to be increased.

[0011] By adopting the technical scheme, the temperature comparison unit can compare the temperatures detected by the first detection module and the second detection module, so that when the temperature detected by the first detection module is greater than the temperature detected by the second detection module, the control module judges that the temperature of the heating chamber is less than the temperature of the bottom plate, so as to control the heating fan to increase its heating power, thereby increasing the temperature in the heating chamber, thereby increasing the temperature near the bottom, and effectively ensuring the heating uniformity of the biological reaction bag.

[0012] Preferably, the side of the housing is provided with an opening, and the housing is also provided with a switch door for closing the opening on the housing. The temperature control mechanism also includes a door detection module, which is communicatively connected to the control module. The door detection module is used to detect whether the switch door is open. The control module includes a temperature compensation unit, which is used to control the heating fan to increase the heating power when the door detection module detects that the switch door is open.

[0013] By adopting the above technical solution and configuring the door detection module, the door detection module can detect whether the door is open or closed. When the door is detected to be open, the temperature compensation unit can control the heating fan to increase the heating power, thereby allowing the external gas to come into contact with the gas inside the heating chamber. After the air inside the heating chamber cools down, the heating fan can quickly heat up the gas inside the heating chamber and restore it to the temperature when it was not open. This effectively ensures the heating effect on the cell reaction bag and guarantees the uniformity of heating of the cell reaction bag.

[0014] Preferably, the chamber heating mechanism further includes an air inlet pipe, a uniform distribution box, and a uniform distribution cover. The uniform distribution box is disposed inside the heating chamber. One end of the air inlet pipe is connected to the air outlet of the heating fan, and the other end is connected to the uniform distribution box. The top of the uniform distribution box is open. The uniform distribution cover is fitted onto the top of the uniform distribution box, and several ventilation holes are provided through the top. The air inlet pipe is connected to the heating chamber through the ventilation holes.

[0015] By adopting the above technical solution, the uniform box and uniform cover are designed so that the gas heated by the heating fan can enter the uniform box after entering the air inlet pipe, and then enter the heating chamber through the ventilation hole on the top of the uniform cover. This allows the gas in the heating pipe to be evenly dispersed, thereby effectively increasing the temperature uniformity in the heating chamber and ensuring the uniform heating of the cell reaction bag.

[0016] Preferably, a guiding mechanism is also provided in the heating cavity. The guiding mechanism includes a guide plate and a linkage component. One end of the guide plate is rotatably connected to the housing. The uniform cover drives the guide plate to rotate through the linkage component, so that the extension direction of the guide plate is tilted and faces the switch door.

[0017] By adopting the above technical solution and setting the guiding mechanism, when the uniform cover rotates, the uniform cover can drive the guide plate to rotate through the linkage, thereby changing the extension direction of the guide plate and pointing it toward the location of the switch door. As a result, a portion of the gas passing through the ventilation holes on the uniform cover can flow toward the position near the switch door after contacting the guide plate, thereby heating the area near the switch door and rapidly increasing the temperature of the lower temperature area near the switch door, effectively ensuring the uniformity of the temperature in the heating chamber.

[0018] Preferably, the guide plate is further provided with a flow guide plate, which is rotatably connected to the guide plate and is used to guide the gas passing through the uniform cover after rotation. The flow guide plate is also provided with a linkage mechanism, which includes a sliding frame, a linkage rod and a gear and rack assembly. The sliding frame is slidably connected to the guide plate, one end of the linkage rod is rotatably connected to the sliding frame and the other end is rotatably connected to the housing. The sliding frame drives the flow guide plate to rotate through the gear and rack assembly.

[0019] By adopting the above technical solution, the design of the guide plate and the linkage mechanism allows the guide plate to be in a retracted state when it is not tilted. This eliminates the guide plate's influence on the gas exiting through the uniform cover, allowing the gas to diffuse evenly into the heating chamber, effectively ensuring the temperature uniformity within the heating chamber and thus guaranteeing the uniform heating of the cell reaction bag. Simultaneously, the linkage mechanism enables the guide plate to automatically drive the sliding frame as it rotates. This, in turn, allows the sliding frame to automatically rotate the guide plate via a gear and rack assembly, effectively achieving linkage between the guide plate and the flow guide plate. This saves on the active components required to drive the flow guide plate and facilitates the control of its rotation.

[0020] Preferably, the linkage component includes a linkage frame, one end of which is rotatably connected to the guide plate and the other end of which is rotatably connected to the uniform cover.

[0021] By adopting the above technical solution and setting the linkage frame, when the uniform cover rotates, the uniform cover can drive the linkage frame to move, thereby causing the linkage frame to drive the guide plate to rotate, realizing the linkage between the uniform cover and the guide plate. This effectively saves the active device for driving the guide plate to rotate, facilitates the operation of relevant personnel, and saves the space required for setting the active device.

[0022] Preferably, the end of the uniform cover near the opening of the housing is rotatably connected to the housing. A rotating component is also provided inside the housing. The rotating component is used to drive the uniform cover to rotate and is communicatively connected to the control module. The control module also includes a region compensation unit. The region compensation unit is used to control the rotating component to drive the uniform cover to rotate when the door detection module detects that the door has been opened and then closed again, and the heating fan is in operation, so that the ventilation hole faces the door.

[0023] By adopting the above technical solution and setting the regional compensation unit, when the door detection module detects that the door has been opened and then closed again, it can control the rotating component to drive the uniform cover to rotate, so that the ventilation holes on the uniform cover are oriented towards the position of the door. This allows the gas vented by the uniform cover to be blown towards the vicinity of the door, thereby effectively increasing the temperature near the door and thus effectively improving the temperature uniformity in the heating chamber, ensuring the uniform heating of the cell reaction bag.

[0024] Preferably, the housing is also provided with an alarm module, which is communicatively connected to the control module. The control module is used to control the alarm module to issue an alarm signal when the door detection module detects that the door has been in an open state for a long time and the heating fan is in operation.

[0025] By adopting the above technical solution and configuring the alarm module, when the door detection module detects that the door has been open for a long time and the heating fan is in operation, the control module can send an alarm signal by controlling the alarm module. This allows relevant personnel to know that the door has been open for a long time, effectively reducing the probability of the door being open for an extended period.

[0026] In summary, this application includes at least one of the following beneficial technical effects: 1. The chamber heating mechanism is designed so that the heating fan can draw in and heat the outside gas, and then introduce the heated gas into the heating chamber. This allows the gas in the heating chamber to heat the surface of the cell reaction bag except for the bottom through heat transfer, thereby increasing the heating uniformity of the cell reaction bag and effectively ensuring the heating effect of the cell reaction bag. 2. The temperature control mechanism is designed so that the first temperature detection module can detect the temperature at the bottom of the cell reaction bag, and the second temperature detection module can detect the temperature inside the heating chamber. This allows the control module to receive the temperatures from the first and second temperature detection modules and, based on the detected temperatures, control the heating power of the bottom heater and the heating fan, thereby regulating the heating effect of the bottom heater and the heating fan and effectively ensuring the heating effect of the cell reaction bag. 3. The setting of the regional compensation unit enables the rotating component to drive the uniform cover to rotate when the door detection module detects that the door has been opened and then closed again. This causes the ventilation holes on the uniform cover to face the location of the door, so that the gas vented by the uniform cover can be blown towards the vicinity of the door, thereby effectively increasing the temperature near the door and thus effectively improving the temperature uniformity in the heating chamber, ensuring the uniform heating of the cell reaction bag. Attached Figure Description

[0027] Figure 1 This is a schematic diagram used to illustrate the overall structure in Embodiment 1 of this application.

[0028] Figure 2 This is a schematic diagram illustrating the structure in Embodiment 1 of this application.

[0029] Figure 3 This is a schematic diagram illustrating the structure in Embodiment 1 of this application.

[0030] Figure 4 This is a structural block diagram illustrating the electrical connection of the control module in Embodiment 1 of this application.

[0031] Figure 5 This is a schematic diagram illustrating the structure of the rotating component in Embodiment 2 of this application.

[0032] Figure 6 This is a structural block diagram illustrating the electrical connection of the control module in Embodiment 2 of this application.

[0033] Figure 7 This is a schematic diagram of the structure of the uniform cover in Embodiment 2 of this application.

[0034] Figure 8 This is a schematic diagram illustrating the structure of the sliding frame in Embodiment 2 of this application.

[0035] Explanation of reference numerals in the attached drawings: 1. Shell; 11. Heating chamber; 12. Placement chamber; 2. Base plate; 3. Bottom heater; 4. Chamber heating mechanism; 41. Heating fan; 42. Air inlet pipe; 43. Uniform distribution box; 44. Uniform distribution cover; 5. Ventilation hole; 6. Temperature control mechanism; 61. First temperature detection module; 62. Second temperature detection module; 63. Control module; 631. Temperature comparison unit; 632. Temperature compensation unit; 633. Area compensation unit; 64. Door detection module; 65. Alarm module; 7. Door opening and closing; 8. Rotating component; 9. Guide mechanism; 91. Guide plate; 911. Extension; 92. Linkage component; 921. Linkage frame; 93. Guide plate; 10. Linkage mechanism; 101. Sliding frame; 102. Linkage rod; 103. Gear and rack assembly; 1031. Linkage rack; 1032. Linkage gear; 1033. Linkage bevel gear. Detailed Implementation

[0036] The following is in conjunction with the appendix Figures 1-8 This application will be described in further detail.

[0037] Example 1: Embodiment 1 of this application discloses a cell culture reactor with fully temperature-controlled operation. (Refer to...) Figure 1 and Figure 2 The fully temperature-controlled cell culture reactor includes a shell 1, within which a heating chamber 11 is provided. A bottom plate 2 and a bottom heater 3 are installed within the heating chamber 11. The bottom plate 2 is used to place cell reaction bags, and the bottom heater 3 is used to heat the bottom plate 2. A chamber heating mechanism 4 is also provided within the shell 1, including a heating fan 41, which is used to heat the gas within the heating chamber 11.

[0038] Reference Figure 2 The housing 1 also includes a placement cavity 12, located directly below the heating cavity 11. The heating fan 41 is bolted to the inner bottom wall of the placement cavity 12. The chamber heating mechanism 4 also includes two air inlet pipes 42, located on opposite sides of the heating fan 41. One end of each air inlet pipe 42 is connected to the air inlet and outlet of the heating fan 41, respectively, and the other ends of each air inlet pipe 42 extend to both ends of the placement cavity 12 along the length of the housing 1 and are bent upwards.

[0039] Reference Figure 2 The chamber heating mechanism 4 also includes a homogenizing box 43. In this embodiment, the number of homogenizing boxes 43 is set to two, and they are respectively located at both ends of the heating chamber 11 along the length of the housing 1. Each homogenizing box 43 is fixedly installed on the inner top wall of the placement chamber 12, and its bottom is connected to the upward-extending end of the corresponding air inlet pipe 42.

[0040] Reference Figure 2 and Figure 3 Each uniform box 43 has an opening at the top, and the inner bottom wall of the heating chamber 11 is also raised upward. Several ventilation holes 5 are provided through it, and each ventilation hole 5 is connected to the opening of the corresponding uniform box 43, so that one uniform box 43 is supplied with gas from the heating chamber 11 through the ventilation hole 5, and the other uniform box 43 is supplied with gas heated by the heating fan 41 through the ventilation hole 5, which is then discharged back into the heating chamber 11.

[0041] Reference Figure 2In this embodiment, the base plate 2 is made of aluminum. The base plate 2 is fixedly mounted to the bottom of the heating chamber 11 via a bracket. The bottom heater 3 is a plate heater located below the base plate 2 for heating the base plate 2. The cell reaction bag is placed on the base plate 2, so that the base plate 2 heats the bottom of the cell reaction bag through heat transfer.

[0042] Reference Figure 2 and Figure 4 The housing 1 also includes a temperature control mechanism 6, which comprises a first temperature detection module 61, a second temperature detection module 62, and a control module 63. In this embodiment, the control module 63 is configured as a PLC controller, the first temperature detection module 61 is configured as a temperature sensor, which is mounted on the base plate 2 and located directly below the cell reaction bag, and the second temperature detection module 62 is configured as a temperature sensor, which is mounted on the outer end of the base plate 2 or on the inner wall of the housing 1. The PLC controller is fixedly mounted on the inner bottom wall of the placement cavity 12, and each of the aforementioned temperature sensors, the heating fan 41, and the bottom heater 3 is communicatively connected to the PLC controller.

[0043] Reference Figure 2 and Figure 4 The first temperature detection module 61 is used to detect the temperature of the base plate 2, and the second temperature detection module 62 is used to detect the indoor temperature of the heating chamber 11. Both the first temperature detection module 61 and the second temperature detection module 62 are used to feed back the detected temperature values ​​to the PLC controller. The PLC controller is used to receive the detected temperature values, and the PLC controller pre-stores the correspondence between the temperature detected by the first temperature detection module 61 and the heating power of the bottom heater 3, and also stores the correspondence between the temperature detected by the second temperature detection module 62 and the heating power of the heating fan 41. After receiving the data detected by the first temperature detection module 61 or the second temperature detection module 62, the PLC controller is used to adjust the heating power of the bottom heater 3 and the heating fan 41 according to the corresponding correspondence.

[0044] Reference Figure 4 The control module 63 also includes a temperature comparison unit 631, which is used to compare the temperature data detected by the first temperature detection module 61 and the second temperature detection module 62, and control the heating fan 41 to increase its heating power when the temperature detected by the first temperature detection module 61 is greater than the temperature detected by the second temperature detection module 62.

[0045] Reference Figure 2The housing 1 has an opening on one side along its width, and a switch door 7 is provided on the housing 1 to close the opening. The top of the switch door 7 is rotatably connected to the top of the housing 1 by a hinge or pin, and the bottom of the switch door 7 may also be provided with a buckle to ensure the effect of the switch door 7 when closed.

[0046] The implementation principle of a fully temperature-controlled cell culture reactor in Embodiment 1 of this application is as follows: When it is necessary to heat the cell reaction bags on the base plate 2, the bottom heater 3 heats the base plate 2, thereby heating the cell reaction bags placed on it through heat transfer. Simultaneously, the heating fan 41 draws in and heats the gas in the heating chamber 11 through one air inlet pipe 42, and after heating, it is introduced into the heating chamber 11 through another air inlet pipe 42, thus heating the gas in the heating chamber 11. This allows the gas in the heating chamber 11 to heat the remaining parts of the cell reaction bags through heat transfer. During this process, the first temperature detection module 61 detects the temperature of the base plate 2, and the second temperature detection module 62 detects the temperature inside the heating chamber 11, thereby regulating the temperatures of the base plate 2 and the heating chamber 11.

[0047] Example 2: The difference between Embodiment 2 and Embodiment 1 in this application is that: (Refer to...) Figure 5 and Figure 6 The temperature control mechanism 6 also includes a door detection module 64. In this embodiment, the door detection module 64 is configured as a proximity switch and a trigger target (metal target). The proximity switch is fixedly installed inside the housing 1, and the trigger target is fixedly installed at the bottom of the door 7. The control module 63 also includes a temperature compensation unit 632, and the proximity switch is communicatively connected to the temperature compensation unit 632.

[0048] Reference Figure 5 and Figure 6 The proximity switch is used to detect whether the trigger target is located within its own sensing area (the trigger target is located within the sensing area when the door 7 is closed), and feeds back the detection data (i.e., whether the output state has changed) to the temperature compensation unit 632. The temperature compensation unit 632 has a pre-stored compensation power value for the heating fan 41. The temperature compensation unit 632 is used to receive the data detected by the proximity switch and to control the heating fan 41 to increase the heating power to reach the compensation power value when the proximity switch detects that the trigger target is not in the sensing area (i.e., when the door 7 is open).

[0049] Reference Figure 5 and Figure 6The housing 1 is also equipped with an alarm module 65. In this embodiment, the alarm module 65 is configured as an alarm light, which is fixedly installed outside the housing 1 and communicatively connected to the PLC controller. The PLC controller also stores an alarm value in advance, which is set as the time value for the door 7 to be in the open state. When the heating fan 41 is in operation and the proximity switch detects that the trigger target is not in the sensing area, and the time it is not in the sensing area exceeds the alarm value, the PLC controller controls the alarm light to work and emits an alarm light as an alarm signal, thereby reminding relevant personnel that the door 7 has not been closed for a long time.

[0050] Reference Figure 5 and Figure 7 The uniform distribution box 43, located above the air inlet pipe 42 which connects to the outlet of the heating fan 41, has an opening at its top and is fixedly mounted on the inner bottom wall of the heating chamber 11. The chamber heating mechanism 4 also includes a uniform distribution cover 44, which is positioned above the uniform distribution box 43 and serves to cover the opening of the box. The top of the uniform distribution cover 44 has several ventilation holes 5, allowing the cover to communicate with the heating chamber 11.

[0051] Reference Figure 5 and Figure 7 The uniform cover 44 extends downwards from its side to cover the corresponding uniform box 43 and is in contact with the side wall of the corresponding uniform box 43. The end of the uniform cover 44 near the switch door 7 is rotatably connected to the frame via a pin. A rotating component 8 is also provided inside the heating chamber 11. In this embodiment, the rotating component 8 is a rotating cylinder. The cylinder body of the rotating cylinder is rotatably connected to the inner bottom wall of the heating chamber 11 via a pin, and the piston rod of the rotating cylinder is rotatably connected to the uniform cover 44 via a pin to drive the rotation of the uniform cover 44.

[0052] Reference Figure 5 and Figure 6 The control module 63 also includes a region compensation unit 633, to which the aforementioned rotating cylinder is communicatively connected. The region compensation unit 633 is used to record the data signal fed back by the proximity switch. When the proximity switch detects that the door 7 is closed, and the previous state of the door 7 detected by the proximity switch was open, and the heating fan 41 is still in operation, the region compensation unit 633 controls the rotating cylinder to operate, thereby causing the rotating cylinder to drive the uniform cover 44 to rotate, so that the ventilation holes 5 on the uniform cover 44 face the vicinity of the door 7.

[0053] Reference Figure 5 and Figure 7The heating chamber 11 is also equipped with a guide mechanism 9, which includes a guide plate 91 and a linkage 92. The guide plate 91 is located directly above the uniform cover 44, and the end of the guide plate 91 near the switch door 7 is rotatably connected to the housing 1 by a pin. The end of the guide plate 91 near the switch door 7 extends downward to an extension 911, which is integrally formed with the guide plate 91, and the bottom end of the extension 911 is inclined away from the switch door 7.

[0054] Reference Figure 5 and Figure 7 The linkage component 92 includes a linkage frame 921. One end of the linkage frame 921 is rotatably connected to the end of the extension 911 away from the switch door 7 via a pin. The other end of the linkage frame 921 is rotatably connected to the side of the uniform cover 44 away from the switch door 7 via a pin.

[0055] Reference Figure 7 and Figure 8 The guide plate 91 is also provided with a flow guide plate 93, the bottom end of which is rotatably connected to the guide plate 91 via a pin. The flow guide plate 93 is also provided with a linkage mechanism 10, which includes a sliding frame 101, a linkage rod 102, and a gear and rack assembly 103. The sliding frame 101 is slidably connected to the guide plate 91 via a slide rail, and the sliding direction is the length direction of the guide plate 91.

[0056] Reference Figure 5 and Figure 8 One end of the linkage rod 102 is rotatably connected to the sliding frame 101 via a pin, and the other end is rotatably connected to the inner wall of the housing 1 via a pin. The gear and rack assembly 103 includes a linkage rack 1031, a linkage gear 1032, and two linkage bevel gears 1033. The linkage rack 1031 is fixedly connected to the sliding frame 101, and its extension direction is the sliding direction of the sliding frame 101. The linkage gear 1032 is located below the linkage rack 1031 and meshes with the linkage rack 1031.

[0057] Reference Figure 7 and Figure 8 The linkage gear 1032 and its linkage bevel gear 1033 are fixedly sleeved on the same pin shaft, which is rotatably connected to the guide plate 91. The other linkage bevel gear 1033 is fixedly sleeved on the end of the guide plate 93, and the axis of this linkage bevel gear 1033 is the same as the rotation axis of the guide plate 93. The two linkage bevel gears 1033 mesh, allowing the sliding frame 101 to drive the guide plate 93 to rotate via the gear and rack assembly 103.

[0058] Reference Figure 5 , Figure 7 and Figure 8In the initial state, the ventilation holes 5 on the uniform cover 44 are vertically upward, and the end of the guide plate 91 away from the switch door 7 is not tilted downward. At this time, the end of the guide plate 93 away from the guide plate 91 is upward. When the rotating component 8 drives the uniform cover 44 to rotate, so that the ventilation holes 5 on the uniform cover 44 gradually face the switch door 7, the uniform cover 44 drives the guide plate 91 to rotate through the linkage component 92, and so that the end of the guide plate 91 away from the switch door 7 gradually tilts.

[0059] Reference Figure 5 , Figure 7 and Figure 8 During this process, the linkage rod 102 drives the sliding frame 101 to slide, causing the sliding frame 101 to gradually approach the linkage gear 1032. At this time, the linkage rack 1031 drives the linkage gear 1032 to rotate, thereby causing the linkage gear 1032 to drive the guide plate 93 to rotate through the two linkage bevel gears 1033. When the ventilation hole 5 on the uniform cover 44 is facing the location of the switch door 7, the end of the guide plate 91 away from the switch door 7 abuts against the top of the uniform cover 44. At this time, the top wall of the guide plate 93 is coplanar with the top wall of the guide plate 91 to guide the gas coming out of the ventilation hole 5.

[0060] The implementation principle of a fully temperature-controlled cell culture reactor in Embodiment 2 of this application is as follows: When the switch door 7 is opened and then closed, the control module 63 controls the rotating component 8 to drive the uniform cover 44 to rotate, so that the ventilation holes 5 on the uniform cover 44 gradually move towards the location of the switch door 7. The uniform cover 44 drives the guide plate 91 to rotate through the linkage component 92, and the end of the guide plate 91 away from the switch door 7 gradually tilts.

[0061] During this process, the linkage rod 102 drives the sliding frame 101 to slide, causing the sliding frame 101 to gradually approach the linkage gear 1032. At this time, the linkage rack 1031 drives the linkage gear 1032 to rotate, thereby causing the linkage gear 1032 to drive the guide plate 93 to rotate through the two linkage bevel gears 1033. When the ventilation hole 5 on the uniform cover 44 is facing the location of the switch door 7, the end of the guide plate 91 away from the switch door 7 abuts against the top of the uniform cover 44. At this time, the top wall of the guide plate 93 is coplanar with the top wall of the guide plate 91 to guide the gas coming out of the ventilation hole 5.

[0062] The above are all preferred embodiments of this application, and are not intended to limit the scope of protection of this application. Therefore, all equivalent changes made in accordance with the structure, shape and principle of this application should be covered within the scope of protection of this application.

Claims

1. A cell culture reactor with fully temperature-controlled operation, comprising a shell (1), wherein a heating chamber (11) is provided inside the shell (1), and a bottom plate (2) and a bottom heater (3) are provided inside the heating chamber (11), wherein the bottom plate (2) is used for placing cell reaction bags, and the bottom heater (3) is used for heating the bottom plate (2), characterized in that: The housing (1) is also provided with a chamber heating mechanism (4), which includes a heating fan (41) for heating the gas in the heating chamber (11).

2. The cell culture reactor with fully temperature-controlled operation according to claim 1, characterized in that: The housing (1) is also provided with a temperature control mechanism (6). The temperature control mechanism (6) includes a first temperature detection module (61), a second temperature detection module (62), and a control module (63). The first temperature detection module (61), the second temperature detection module (62), the bottom heater (3), and the heating fan (41) are all communicatively connected to the control module (63). The first temperature detection module (61) is used to detect the temperature of the bottom plate (2), the second temperature detection module (62) is used to detect the temperature inside the heating cavity (11), and the control module (63) is used to control the heating power of the bottom heater (3) and the heating fan (41) based on the temperatures detected by the first temperature detection module (61) and the second temperature detection module (62).

3. The cell culture reactor with fully temperature-controlled operation according to claim 2, characterized in that: The control module (63) further includes a temperature comparison unit (631), which is used to compare the temperature data detected by the first temperature detection module (61) and the second temperature detection module (62), and control the heating fan (41) to increase the heating power when the temperature detected by the first temperature detection module (61) is greater than the temperature detected by the second temperature detection module (62).

4. The cell culture reactor with fully temperature-controlled operation according to claim 2, characterized in that: The side of the housing (1) is also provided with an opening, and the housing (1) is also provided with a switch door (7). The switch door (7) is used to close the opening on the housing (1). The temperature control mechanism (6) also includes a door detection module (64). The door detection module (64) is communicatively connected to the control module (63). The door detection module (64) is used to detect whether the switch door (7) is open. The control module (63) includes a temperature compensation unit (632). The temperature compensation unit (632) is used to control the heating fan (41) to increase the heating power when the door detection module (64) detects that the switch door (7) is open.

5. A cell culture reactor with fully temperature-controlled operation according to claim 4, characterized in that: The chamber heating mechanism (4) further includes an air inlet pipe (42), a uniform box (43), and a uniform cover (44). The uniform box (43) is disposed inside the heating chamber (11). One end of the air inlet pipe (42) is connected to the air outlet of the heating fan (41), and the other end is connected to the uniform box (43). The top of the uniform box (43) is open. The uniform cover (44) is fitted onto the top of the uniform box (43), and several ventilation holes (5) are opened through the top. The air inlet pipe (42) is connected to the heating chamber (11) through the ventilation holes (5).

6. A cell culture reactor with fully temperature-controlled operation according to claim 5, characterized in that: The heating chamber (11) is also provided with a guide mechanism (9), which includes a guide plate (91) and a linkage (92). One end of the guide plate (91) is rotatably connected to the housing (1). The uniform cover (44) drives the guide plate (91) to rotate through the linkage (92), so that the extension direction of the guide plate (91) is tilted and faces the switch door (7).

7. A cell culture reactor with fully temperature-controlled operation according to claim 6, characterized in that: A flow guide plate (93) is also provided on the guide plate (91). The flow guide plate (93) is rotatably connected to the guide plate (91) and is used to guide the gas passing through the uniform cover (44) after rotation. A linkage mechanism (10) is also provided on the flow guide plate (93). The linkage mechanism (10) includes a sliding frame (101), a linkage rod (102), and a gear and rack assembly (103). The sliding frame (101) is slidably connected to the guide plate (91). One end of the linkage rod (102) is rotatably connected to the sliding frame (101), and the other end is rotatably connected to the housing (1). The sliding frame (101) drives the flow guide plate (93) to rotate through the gear and rack assembly (103).

8. A cell culture reactor with fully temperature-controlled operation according to claim 6, characterized in that: The linkage component (92) includes a linkage frame (921), one end of which is rotatably connected to the guide plate (91), and the other end is rotatably connected to the uniform cover (44).

9. A cell culture reactor with fully temperature-controlled operation according to claim 5, characterized in that: The uniform cover (44) is rotatably connected to the housing (1) at one end near the opening of the housing (1). A rotating component (8) is also provided inside the housing (1). The rotating component (8) is used to drive the uniform cover (44) to rotate and is communicatively connected to the control module (63). The control module (63) also includes a region compensation unit (633). The region compensation unit (633) is used to control the rotating component (8) to drive the uniform cover (44) to rotate when the door detection module (64) detects that the door (7) has been opened and then closed again, and the heating fan (41) is in working condition, so that the ventilation hole (5) faces the door (7).

10. A cell culture reactor with fully temperature-controlled operation according to claim 9, characterized in that: An alarm module (65) is also provided on the housing (1). The alarm module (65) is communicatively connected to the control module (63). The control module (63) is used to control the alarm module (65) to issue an alarm signal when the door detection module (64) detects that the door (7) has been in an open state for a long time and the heating fan (41) is in a working state.

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