Sterile fermentation device for sophorolipid

Abstract

The utility model provides a sophorolipid sterile fermentation device which comprises a tank body, a jacket, a steam inlet pipe, a steam outlet pipe, a cooling water inlet pipe, a cooling water outlet pipe, a liquid discharge pipe, a gas inlet pipe and a detection control system, and the detection control system is used for collecting and adjusting internal environment data of the tank body. The sophorolipid fermentation device has a plurality of temperature control modes, is convenient in temperature regulation, adopts the double temperature control modes of the electric heating pipe and steam heating, and is combined with a cooling water cooling system, so that the temperature regulation is more flexible, the accurate control of the temperature in the fermentation process is improved, and the optimal fermentation environment of sophorolipid is favorably maintained. The liquid level sensor, the pressure sensor, the temperature sensor, the pH sensor and the dissolved oxygen sensor are adopted, key parameters of fermentation liquor can be collected in real time, accurate monitoring of the fermentation environment is ensured, the fermentation efficiency and the product quality can be improved, and good economic benefits are achieved.

Description

TECHNICAL FIELD

[0001] The utility model relates to fermentation technical field, concretely is a sophorolipid sterile fermentation device. BACKGROUND

[0002] In modern biological engineering and pharmaceutical industry, fermentation technology is widely used in the production of various biological products, such as antibiotics, enzyme preparations and bioactive substances. Sophorolipid, as an important biological surfactant, has good biocompatibility and biodegradability, and has been paid more and more attention in the fields of food, medicine and cosmetics. However, the production process of sophorolipid usually needs to be carried out under strict sterile conditions to avoid microbial contamination and improve the quality of products.

[0003] The current sophorolipid sterile fermentation device has the problem of single temperature control mode. The conventional fermentation tank usually adopts single steam heating temperature control mode, and the temperature regulation response lag is obvious. The temperature rising and falling speed is slow. Moreover, the sensor is less, and the collection of fermentation environment data is not enough, which will lead to insufficient monitoring of the fermentation process. UTILITY MODEL CONTENT

[0004] The utility model aims at providing a sophorolipid sterile fermentation device, which has multiple temperature control modes and diversified data collection, and can monitor the fermentation environment in real time.

[0005] In order to achieve the above purpose, the utility model provides the following technical scheme: a sophorolipid sterile fermentation device, comprising:

[0006] The tank body is provided with a stirring mechanism at the top;

[0007] The jacket is wrapped outside the tank body, and the jacket and the tank body have a temperature control cavity therebetween, and an electric heating pipe is arranged in the temperature control cavity, which is used for heating the tank body;

[0008] The steam inlet pipe and the steam outlet pipe are communicated with the temperature control cavity, and the first electromagnetic valve is arranged on the steam inlet pipe;

[0009] The cooling water inlet pipe and the cooling water outlet pipe are communicated with the temperature control cavity, and the second electromagnetic valve is arranged on the cooling water inlet pipe;

[0010] The liquid discharge pipe is communicated with the bottom of the tank body, and the gas inlet pipe is communicated with the tank body;

[0011] The detection control system is used for collecting and adjusting the internal environment data of the tank body.

[0012] Further, in the utility model, the tank body is stainless steel tank body, the inner wall electrolytic polishing, Ra≤.μm.

[0013] Further, in the utility model, the stirring mechanism includes stirring motor and stirring rod, the stirring motor is installed at the top of tank body, and the output shaft of stirring motor is fixedly connected with stirring rod.

[0014] Further, in the utility model, the top of tank body is provided with feed pipe, and sight glass and material supplementing port are arranged on the feed pipe.

[0015] Further, in the utility model, the tank body is communicated with sampling pipe, and sealing valve is arranged on the sampling pipe.

[0016] Further, in the utility model, the gas inlet pipe is communicated with sterile gas source.

[0017] Further, in the utility model, the top of tank body is communicated with exhaust pipe, and exhaust valve is arranged on the exhaust pipe.

[0018] Further, in the utility model, the detection control system includes:

[0019] Liquid level sensor, the liquid level sensor is used for detecting the liquid level data in tank body, and the filling condition of fermentation broth is monitored in real time, the liquid level sensor is installed on the inner wall of tank body and is connected with singlechip, and liquid level signal is transmitted to singlechip for processing and display;

[0020] Pressure sensor, the pressure sensor is used for detecting the pressure in tank body, and the pressure change in fermentation process is monitored in real time, the pressure sensor is installed on the top or side wall of tank body and is connected with singlechip, and pressure signal is transmitted to singlechip for processing and monitoring;

[0021] Temperature sensor, the temperature sensor is used for detecting the temperature of fermentation broth in tank body, and the temperature change in fermentation process is monitored in real time, the temperature sensor is installed on the detection frame on the inner wall of tank body and is connected with singlechip, and temperature signal is transmitted to singlechip for processing and control;

[0022] PH sensor, the PH sensor is used for detecting the PH value of fermentation broth in tank body, and the pH change in fermentation process is monitored in real time, the PH sensor is installed on the detection frame on the inner wall of tank body and is connected with singlechip, and PH value signal is transmitted to singlechip for processing and control;

[0023] Dissolved oxygen sensor, the dissolved oxygen sensor is used for detecting the dissolved oxygen content of fermentation broth in tank body, and the oxygen supply condition in fermentation process is monitored in real time, the dissolved oxygen sensor is installed on the detection frame on the inner wall of tank body and is connected with singlechip, and dissolved oxygen signal is transmitted to singlechip for processing and control;

[0024] The single-chip microcomputer is used for receiving and processing data from the liquid level sensor, the temperature sensor, the PH sensor, the dissolved oxygen sensor and the pressure sensor, and controlling the operation of the first electromagnetic valve, the second electromagnetic valve and the electric heating tube according to a preset control strategy, so as to adjust the liquid temperature in the tank body, and the single-chip microcomputer stores the collected data into the storage module and sends the data to the user end through the wireless communication module.

[0025] Further, the storage module is used for storing the liquid level, temperature, PH value, dissolved oxygen and pressure data collected by the single-chip microcomputer, so as to facilitate subsequent data analysis and process tracing, and the storage module is connected with the single-chip microcomputer, receives and stores the data sent by the single-chip microcomputer.

[0026] The first electromagnetic valve is used for controlling the opening and closing of the steam inlet pipe, so as to adjust the steam amount entering the temperature control cavity and realize heating control of the tank body, and the first electromagnetic valve is connected with the single-chip microcomputer, receives the control signal of the single-chip microcomputer and controls the opening and closing of the steam inlet pipe.

[0027] The second electromagnetic valve is used for controlling the opening and closing of the cooling water inlet pipe, so as to adjust the cooling water amount entering the temperature control cavity and realize cooling control of the tank body, and the second electromagnetic valve is connected with the single-chip microcomputer, receives the control signal of the single-chip microcomputer and controls the opening and closing of the cooling water inlet pipe.

[0028] The electric heating tube is used for temperature control heating of the tank body, and the electric heating tube is connected with the single-chip microcomputer, receives the control signal of the single-chip microcomputer and controls the power of the electric heating tube, so as to adjust the heating amount.

[0029] Further, the wireless communication module is used for sending the data and control information collected by the single-chip microcomputer to the user end, realizing remote monitoring, and the wireless communication module is connected with the single-chip microcomputer, receives the data and control information sent by the single-chip microcomputer and sends them to the user end through a wireless network.

[0030] The user end is a computer, a mobile phone or other smart devices, which is used for receiving the data and control information from the wireless communication module and displaying the liquid level, temperature, PH value, dissolved oxygen and pressure data in the tank body.

[0031] Beneficial effects, the technical scheme of the application has the following technical effects:

[0032] The utility model discloses a variety of temperature control mode, temperature regulation is convenient, the device adopts electric heating tube and steam heating double temperature control mode, and combines cooling water cooling system, make temperature regulation more flexible, improve the precision control of temperature in the fermentation process, help to maintain sophorolipid fermentation The optimal environment. Adopt liquid level sensor, pressure sensor, temperature sensor, pH sensor and dissolved oxygen sensor, can real -time acquisition of the key parameters of fermentation broth, ensure the accurate monitoring of fermentation environment, help to improve fermentation efficiency and product quality, with very good economic benefit.

[0033] It will be appreciated that all combinations of the foregoing concepts (and additional concepts described below) can be viewed as being part of the inventive subject matter herein unless otherwise stated or otherwise understood from context and / or the specification as a whole.

[0034] The foregoing and other aspects, embodiments and features of the present teachings can be better understood and appreciated from the following description, taken together with the accompanying drawings. Other aspects, embodiments and features of the present teachings will be apparent from the description that follows and from the claims. BRIEF DESCRIPTION OF DRAWINGS

[0035] The drawings are not intended to be drawn to scale. In the drawings, each same or like component that is shown in and described with respect to multiple figures can be indicated with a same reference label. For purposes of clarity, not every component can be labeled in every figure. Embodiments of various aspects of the present teachings will now be described, by way of example only, with reference to the attached figures, in which:

[0036] Figure 1 is a structural schematic view of the utility model.

[0037] Figure 2 is a top view of the partial structure of the utility model.

[0038] Figure 3 is a system block diagram of the utility model.

[0039] In the drawings, the meanings of various reference signs are as follows: 1, tank body;2, jacket;3, temperature control cavity;4, electric heating tube;5, steam inlet pipe;6, steam outlet pipe;7, cooling water inlet pipe;8, cooling water outlet pipe;9, liquid outlet pipe;10, gas inlet pipe;11, stirring mechanism;111, stirring motor;112, stirring rod;12, feed pipe;13, sampling pipe;14, exhaust pipe;15, liquid level sensor;16, pressure sensor;17, detection frame. DETAILED DESCRIPTION

[0040] To better understand the technical content of this utility model, specific embodiments are described below in conjunction with the accompanying drawings. Various aspects of this utility model are described in this disclosure with reference to the accompanying drawings, which illustrate numerous illustrative embodiments. The embodiments of this disclosure are not necessarily defined to include all aspects of this utility model. It should be understood that the various concepts and embodiments described above, as well as those described in more detail below, can be implemented in any of many ways, because the concepts and embodiments disclosed in this utility model are not limited to any particular implementation. Furthermore, some aspects of this utility model can be used alone or in any suitable combination with other aspects disclosed in this utility model.

[0041] Sophorolipids are glycolipid biosurfactants secreted by microorganisms such as *Candida bombicola*. They consist of hydrophobic fatty acid chains linked to hydrophilic sophorose (a disaccharide) via glycosidic bonds, exhibiting an amphiphilic structure. Current sophorolipid fermentation devices suffer from limited temperature control methods and a lack of sensors, potentially leading to lag in temperature regulation and insufficient data monitoring, thus restricting product yield and quality stability. The following example clarifies this issue.

[0042] Example 1

[0043] like Figures 1-2 As shown, a sophorolipid aseptic fermentation device includes a tank 1, a jacket 2, a steam inlet pipe 5, a steam outlet pipe 6, a cooling water inlet pipe 7, a cooling water outlet pipe 8, a drain pipe 9, a gas inlet pipe 10, and a detection and control system. A stirring mechanism 11 is installed on the top of the tank 1. The tank 1 is made of stainless steel with an electropolished inner wall, Ra≤0.4μm. The tank 1 provides the site for the fermentation reaction. The stainless steel material ensures corrosion resistance, easy cleaning, and resistance to high-temperature and high-pressure sterilization. The electropolished inner wall reduces microbial adhesion and improves sterility. As the main container for the fermentation reaction, it ensures a sterile environment during the fermentation process.

[0044] The stirring mechanism 11 includes a stirring motor 111 and a stirring rod 112. The stirring motor 111 is mounted on the top of the tank 1, and the output shaft of the stirring motor 111 is fixedly connected to the stirring rod 112. The stirring motor 111 drives the stirring rod 112 to rotate, making the fermentation broth mix evenly and ensuring uniform mixing of the fermentation broth. This improves mass transfer efficiency.

[0045] The jacket 2 surrounds the outside of the tank body 1, and a temperature control chamber 3 is located between the jacket 2 and the tank body 1, providing space for the electric heating element, steam, and cooling water. To ensure efficient temperature transfer, an electric heating element 4 is installed within the temperature control chamber 3. The electric heating element 4 heats the tank body 1 quickly and responds rapidly. The heating power can be precisely controlled. 304 stainless steel electric heating elements can be used, and the power is determined based on the tank size and heating requirements (e.g., 3kW, 6kW, etc.). The resistance wire heats up when energized, converting electrical energy into heat energy.

[0046] The steam inlet pipe 5 and the steam outlet pipe 6 are in communication with the temperature control cavity 3, the first electromagnetic valve is arranged on the steam inlet pipe 5, the steam heating efficiency is high, the temperature rises fast, and it is suitable for large-scale heating. It can provide rapid temperature rise and temperature maintenance. The pipe diameter and material are determined according to the steam pressure and flow demand. The heat energy carried by the steam is used to heat the tank body.

[0047] The cooling water inlet pipe 7 and the cooling water outlet pipe 8 are in communication with the temperature control cavity 3, the second electromagnetic valve is arranged on the cooling water inlet pipe 7, and the cooling speed is controllable. It is suitable for maintaining low temperature or rapid cooling. It provides cooling function to prevent overheating. The pipe diameter and material are determined according to the cooling water flow demand. The cooling water is used to absorb the heat of the tank body to reduce the temperature.

[0048] The liquid discharge pipe 9 is in communication with the bottom of the tank body 1, and the gas inlet pipe 10 is in communication with the tank body 1. The liquid discharge pipe 9 facilitates the discharge of fermentation liquid. It is convenient for cleaning and maintenance. The gas inlet pipe 10 provides oxygen or other gas required for fermentation, which can be used for stirring and mixing, and provides a gas environment for fermentation. Sterile gas is introduced into the tank body.

[0049] In summary, the jacket 2 provides uniform temperature control, various temperature control methods, including electric heating, steam heating, and cooling water cooling, which improves the flexibility and response speed of temperature control. By arranging the jacket outside the tank body, the heating or cooling medium circulates in the jacket, thereby controlling the temperature of the tank body and accurately controlling the temperature inside the tank body.

[0050] The detection control system is used for collecting and adjusting the internal environment data of the tank body 1. It can monitor the key parameters of the fermentation process in real time. It is helpful to ensure the stability of the fermentation process. Remote monitoring is convenient for management.

[0051] In this embodiment, the top of the tank body 1 is provided with a feed pipe 12, and a sight glass and a feeding port are arranged on the feed pipe 12. It is convenient to add culture medium or other materials. The sight glass can observe the feeding condition.

[0052] In this embodiment, the tank body 1 is communicated with a sampling pipe 13, and a sealing valve is arranged on the sampling pipe 13. It is convenient to take samples for analysis. The sealing valve ensures the sterility.

[0053] In this embodiment, the gas inlet pipe 10 is communicated with a sterile gas source.

[0054] In this embodiment, the top of the tank body 1 is communicated with an exhaust pipe 14, and an exhaust valve is arranged on the exhaust pipe 14. The gas generated by fermentation is discharged. It prevents the pressure in the tank from being too high.

[0055] Embodiment 2

[0056] As shown in Figures 1-3 the detection control system of embodiment 1, the detection control system comprises:

[0057] A liquid level sensor 15 is used to detect the liquid level data in the tank body 1, to monitor the filling of the fermentation liquid in real time, and is installed on the inner wall of the tank body 1 and connected to the single-chip microcomputer to transmit the liquid level signal to the single-chip microcomputer for processing and display. The model of the liquid level sensor 15 is Siemens SITRANS LR200 radar level gauge, which uses microwave radar technology, the sensor transmits 26GHz high-frequency microwaves, receives the reflected waves of the liquid surface, and calculates the liquid level height by time of flight (range 0-10m, accuracy ±2mm). Non-contact measurement avoids contamination and is suitable for sterile environments and high-viscosity fermentation liquids.

[0058] A pressure sensor 16 is used to detect the pressure in the tank body 1, to monitor the pressure changes during the fermentation process in real time, and is installed on the top or side wall of the tank body 1 and connected to the single-chip microcomputer to transmit the pressure signal to the single-chip microcomputer for processing and monitoring. The pressure sensor 16 uses a MEMS piezoresistive sensor. Pressure acting on the silicon diaphragm causes the resistance of the Wheatstone bridge to change, outputting a digital signal (range 0-1MPa, accuracy ±0.25%). The high-temperature design is directly integrated into the top or side wall of the tank, and the pressure fluctuations in the tank are monitored in real time.

[0059] A temperature sensor is used to detect the temperature of the fermentation liquid in the tank body 1, to monitor the temperature changes during the fermentation process in real time, and is installed on the detection frame 17 on the inner wall of the tank body 1 and connected to the single-chip microcomputer to transmit the temperature signal to the single-chip microcomputer for processing and control. The temperature sensor is a PT100, which is based on the characteristic that the resistance of a platinum resistor changes linearly with temperature. PT100 has a resistance of 100Ω at 0°C, and the resistance increases by about 0.385Ω for every 1°C increase in temperature. By measuring the change in resistance, combined with a transmitter to convert to a 4-20mA or digital signal, accurate temperature monitoring is achieved (accuracy ±0.1°C). It has the advantages of high temperature resistance and corrosion resistance, can directly contact the fermentation liquid, and is suitable for sterile environments.

[0060] A pH sensor is used to detect the pH value of the fermentation liquid in the tank body 1, to monitor the changes in acidity and alkalinity during the fermentation process in real time, and is installed on the detection frame 17 on the inner wall of the tank body 1 and connected to the single-chip microcomputer to transmit the pH value signal to the single-chip microcomputer for processing and control. The pH sensor uses Mettler-Toledo InPro 3250, when the pH-sensitive glass membrane comes into contact with the fermentation liquid, the difference in hydrogen ion concentration between the inside and outside of the membrane produces a potential difference, and the reference electrode provides a stable potential. The potential difference is converted to a pH value by a transmitter (range 0-14, accuracy ±0.01). It can be sterilized at high temperatures (resistant to 130°C steam sterilization) and is suitable for installation in fermentation tanks.

[0061] The dissolved oxygen sensor is used for detecting the dissolved oxygen content of the fermentation liquid in the tank body 1, monitoring the oxygen supply in the fermentation process in real time, and is installed on the detection frame 17 on the inner wall of the tank body 1 and connected with the single-chip microcomputer to transmit the dissolved oxygen signal to the single-chip microcomputer for processing and control. The model of the dissolved oxygen sensor is VisiFerm DO 120. The fluorescent substance on the surface of the sensor emits red light after being excited by blue light. The higher the dissolved oxygen concentration is, the shorter the fluorescent lifetime is. The dissolved oxygen content is calculated by measuring the fluorescent decay time (range 0-100% saturation, accuracy ±1%). No electrolyte is needed, the maintenance period is long, it is resistant to bubble interference, and is suitable for long-term online monitoring.

[0062] The single-chip microcomputer is used for receiving and processing data from the liquid level sensor, the temperature sensor, the PH sensor, the dissolved oxygen sensor and the pressure sensor. The single-chip microcomputer controls the operation of the first electromagnetic valve, the second electromagnetic valve and the electric heating tube according to a preset control strategy to adjust the liquid temperature in the tank body. The single-chip microcomputer stores the collected data in the storage module and sends the data to the user end through the wireless communication module. The model of the single-chip microcomputer is STM32F407VGT6 (ARM Cortex-M4 core), which integrates a multi-channel ADC (12 bits, sampling rate 2.4 MSPS) to collect sensor signals, supports PWM output to control heating power, and has UART / SPI interfaces to connect the wireless module. It has the characteristics of low power consumption and high real-time performance, and is suitable for industrial multi-task control.

[0063] The storage module is used for storing the liquid level, temperature, PH value, dissolved oxygen and pressure data collected by the single-chip microcomputer for subsequent data analysis and process tracing. The storage module is connected with the single-chip microcomputer to receive and store the data sent by the single-chip microcomputer.

[0064] The first electromagnetic valve is used for controlling the opening and closing of the steam inlet pipe 5 to adjust the amount of steam entering the temperature control cavity 3 and realize heating control of the tank body 1. The first electromagnetic valve is connected with the single-chip microcomputer to receive the control signal of the single-chip microcomputer and control the opening and closing of the steam inlet pipe 5.

[0065] The second electromagnetic valve is used for controlling the opening and closing of the cooling water inlet pipe 7 to adjust the amount of cooling water entering the temperature control cavity 3 and realize cooling control of the tank body 1. The second electromagnetic valve is connected with the single-chip microcomputer to receive the control signal of the single-chip microcomputer and control the opening and closing of the cooling water inlet pipe 7.

[0066] An electric heating tube is used for temperature control heating of the tank body 1, and is connected with the single-chip microcomputer to receive the control signal of the single-chip microcomputer and control the power of the electric heating tube, so as to adjust the heating amount. The electric heating tube is selected from an SRY6-220V / 3KW type of Aode Machinery, the resistance wire generates heat, the duty cycle is adjusted through the PWM signal of the single-chip microcomputer, and the heating power (0-100% adjustable) is controlled. The stainless steel shell is corrosion-resistant and can be directly immersed in the jacket temperature control cavity. The built-in fuse protection automatically cuts off the power supply when the temperature is too high.

[0067] The wireless communication module is used for sending the data and control information collected by the single-chip microcomputer to the user end to realize remote monitoring. The wireless communication module is connected with the single-chip microcomputer to receive the data and control information sent by the single-chip microcomputer and send them to the user end through a wireless network. The wireless communication module is selected from an ESP32-WROOM-3E, which is a Wi-Fi+Bluetooth dual-mode module. The data is uploaded to a cloud platform through Wi-Fi in an MQTT / HTTP protocol, and the data is displayed on a mobile phone APP or a PC end. The transmission distance is 50 m indoors and 100 m outdoors, and the frequency band is 2.4 GHz.

[0068] The user end is a computer, a mobile phone or other smart devices, which is used for receiving the data and control information from the wireless communication module and displaying the liquid level, temperature, PH value, dissolved oxygen and pressure data in the tank body 1.

[0069] The use process of the sophorolipid sterile fermentation device is as follows. The device is started and initialized, the system is self-checked, the single-chip microcomputer (STM32F407) is started, the state of the tank is detected through the sensors (temperature, pressure, liquid level, etc.), and it is confirmed that there is no abnormality (such as pressure leakage and abnormal liquid level). The electromagnetic valve (steam, water cooling), the electric heating tube and the stirring motor enter the standby mode.

[0070] Before fermentation, sterilization is performed, then feeding and inoculation of the strain are performed, the culture medium is injected through the feeding pipe 12, the sophorolipid producing strain is inoculated under sterile conditions, and the feeding port is sealed.

[0071] During the fermentation process, the temperature is controlled in the bacterial growth stage (0-24 hours), the electric heating tube (SRY6-3KW) is operated, then the electric heating tube is turned off, the temperature is increased from room temperature to 30°C (the PT100 sensor feeds back in real time), the stirring motor 111 is operated at high speed (300-500 rpm) to improve the dissolved oxygen efficiency, the gas inlet pipe 10 is connected with sterile air, and the dissolved oxygen sensor (VisiFerm DO) monitors the oxygen content. If the PH value is lower than 5.5 (the bacteria produce acid), the alkaline buffer is supplemented; if the PH value is higher than 6.5, the acid regulator is injected (supplemented through the feeding pipe).

[0072] Product synthesis stage (24-72 hours), cooling requirement, after the metabolism of the bacteria slows down, the second electromagnetic valve is started, the cooling water is connected to the jacket, and the temperature is reduced to 25 DEG C (to avoid product degradation). The stirring speed is reduced to 200 rpm to reduce the damage of shear force to the bacteria. The pressure sensor (BMP388) detects the pressure in the tank in real time, and when the pressure exceeds 0.3 MPa, the exhaust pipe 14 automatically releases pressure.

[0073] The sensor collects data (temperature, PH, dissolved oxygen, pressure, liquid level) every 10 seconds, which is transmitted to the single-chip microcomputer through the ADC module. The storage module records complete data, and the wireless communication module pushes data to the user end.

[0074] Fermentation termination and product collection, dissolved oxygen continues to rise (bacteria stop consuming oxygen), PH is stable (metabolism is terminated), and it is determined that the fermentation is completed (about 72 hours). The liquid discharge pipe 9 is opened, the fermentation broth is filtered sterilely and then enters the downstream separation and purification process. The sampling pipe 13 collects the final sample, and the off-line detection of sophorolipid concentration and homolog ratio is carried out. The present embodiment has various temperature control modes, and the temperature adjustment is convenient. The device adopts double temperature control modes of electric heating tube and steam heating, and combines with the cooling water cooling system, so that the temperature adjustment is more flexible, the precision control of the temperature in the fermentation process is improved, and the optimal environment for sophorolipid fermentation is maintained. The liquid level sensor, pressure sensor, temperature sensor, pH sensor and dissolved oxygen sensor can collect the key parameters of the fermentation broth in real time, ensure the accurate monitoring of the fermentation environment, help to improve the fermentation efficiency and product quality, and have good economic benefits.

[0075] The standard parts used in the present application file can be purchased from the market, and can be ordered according to the description and drawings, and the specific connection mode of each part adopts the conventional bolt, rivet, welding and other conventional means in the prior art, the mechanical, parts and equipment adopt the conventional type in the prior art, the control mode is automatically controlled through the controller, and the control circuit of the controller can be realized by simple programming of the person skilled in the art, which belongs to the common knowledge in the art, and the present application is mainly used to protect the mechanical device, so the control mode and circuit connection are not explained in detail.

[0076] Although the present application has been disclosed as above with preferred embodiments, it is not intended to limit the present application. Those skilled in the art can make various modifications and improvements without departing from the spirit and scope of the present application. Therefore, the protection scope of the present application shall be subject to the definition of the claims.

Claims

1. A sterile fermentation apparatus for sophorolipids, characterized in that: include: Tank (1), the top of which is provided with a stirring mechanism (11); A jacket (2) is wrapped around the outside of the tank body (1). A temperature control cavity (3) is provided between the jacket (2) and the tank body (1). An electric heating tube (4) is provided in the temperature control cavity (3). The electric heating tube (4) is used to heat the tank body (1). Steam inlet pipe (5) and steam outlet pipe (6) are connected to the temperature control chamber (3), and a first solenoid valve is provided on the steam inlet pipe (5); Cooling water inlet pipe (7) and cooling water outlet pipe (8), the cooling water inlet pipe (7) and cooling water outlet pipe (8) are connected to the temperature control chamber (3), and a second solenoid valve is provided on the cooling water inlet pipe (7); The drain pipe (9) and the gas inlet pipe (10) are connected to the bottom of the tank (1) and the gas inlet pipe (10) is connected to the tank (1). The detection and control system is used to collect and regulate the internal environmental data of the tank (1).

2. The aseptic fermentation apparatus for sophorolipids according to claim 1, characterized in that: The tank (1) is a stainless steel tank with an inner wall that is electropolished and has Ra≤0.4μm.

3. The aseptic fermentation apparatus for sophorolipids according to claim 1, characterized in that: The stirring mechanism (11) includes a stirring motor (111) and a stirring rod (112). The stirring motor (111) is installed on the top of the tank (1), and the output shaft of the stirring motor (111) is fixedly connected to the stirring rod (112).

4. The aseptic fermentation apparatus for sophorolipids according to claim 1, characterized in that: The top of the tank (1) is provided with a feed pipe (12), and the feed pipe (12) is provided with a sight glass and a feeding port.

5. The aseptic fermentation apparatus for sophorolipids according to claim 1, characterized in that: The tank (1) is connected to a sampling tube (13), and a sealing valve is provided on the sampling tube (13).

6. The aseptic fermentation apparatus for sophorolipids according to claim 1, characterized in that: The gas inlet pipe (10) is connected to a sterile gas source.

7. The aseptic fermentation apparatus for sophorolipids according to claim 1, characterized in that: The top of the tank (1) is connected to an exhaust pipe (14), and an exhaust valve is provided on the exhaust pipe (14).

8. The aseptic fermentation apparatus for sophorolipids according to claim 1, characterized in that: The detection and control system includes: Liquid level sensor (15) is used to detect the liquid level data in the tank (1) and monitor the filling status of the fermentation liquid in real time. The liquid level sensor is installed on the inner wall of the tank (1) and connected to the microcontroller to transmit the liquid level signal to the microcontroller for processing and display. Pressure sensor (16) is used to detect the pressure inside the tank (1) and monitor the pressure changes during the fermentation process in real time. The pressure sensor is installed on the top or side wall of the tank (1) and connected to the microcontroller to transmit the pressure signal to the microcontroller for processing and monitoring. Temperature sensor, the temperature sensor is used to detect the temperature of the fermentation liquid in the tank (1) and monitor the temperature change in the fermentation process in real time. The temperature sensor is installed on the detection frame (17) on the inner wall of the tank (1) and connected to the microcontroller to transmit the temperature signal to the microcontroller for processing and control. The pH sensor is used to detect the pH value of the fermentation liquid in the tank (1) and monitor the acidity and alkalinity changes in real time during the fermentation process. The pH sensor is installed on the detection frame (17) on the inner wall of the tank (1) and connected to the microcontroller to transmit the pH value signal to the microcontroller for processing and control. Dissolved oxygen sensor is used to detect the dissolved oxygen content of the fermentation liquid in the tank (1) and monitor the oxygen supply during the fermentation process in real time. The dissolved oxygen sensor is installed on the detection frame (17) on the inner wall of the tank (1) and connected to the microcontroller to transmit the dissolved oxygen signal to the microcontroller for processing and control. The microcontroller receives and processes data from a liquid level sensor, a temperature sensor, a pH sensor, a dissolved oxygen sensor, and a pressure sensor. According to a preset control strategy, the microcontroller controls the operation of a first solenoid valve, a second solenoid valve, and an electric heating element to regulate the liquid temperature inside the tank. The microcontroller stores the collected data in a storage module and sends the data to the user terminal via a wireless communication module.

9. The aseptic fermentation apparatus for sophorolipids according to claim 8, characterized in that: The storage module is used to store liquid level, temperature, pH value, dissolved oxygen and pressure data collected by the microcontroller for subsequent data analysis and process traceability. The storage module is connected to the microcontroller, receives data sent by the microcontroller and stores it. The first solenoid valve is used to control the opening and closing of the steam inlet pipe (5), thereby regulating the amount of steam entering the temperature control chamber (3) and realizing the heating control of the tank (1). The first solenoid valve is connected to the microcontroller and receives the control signal from the microcontroller to control the opening and closing of the steam inlet pipe (5). The second solenoid valve is used to control the opening and closing of the cooling water inlet pipe (7), thereby regulating the amount of cooling water entering the temperature control chamber (3) and realizing the cooling control of the tank (1). The second solenoid valve is connected to the microcontroller and receives the control signal from the microcontroller to control the opening and closing of the cooling water inlet pipe (7). An electric heating tube is used to control the temperature of the tank (1). The electric heating tube is connected to a microcontroller and receives control signals from the microcontroller to control the power of the electric heating tube, thereby adjusting the heating amount.

10. The aseptic fermentation apparatus for sophorolipids according to claim 8, characterized in that: The wireless communication module is used to send the data and control information collected by the microcontroller to the user terminal to realize remote monitoring. The wireless communication module is connected to the microcontroller, receives the data and control information sent by the microcontroller, and sends it to the user terminal through the wireless network. The user terminal is a computer, mobile phone or other smart device used to receive data and control information from the wireless communication module and display the liquid level, temperature, pH value, dissolved oxygen and pressure data in the tank (1).

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