An andro medon peptide-containing alcohol-protecting and liver-protecting food-medicine homologous composition and a preparation method thereof

Abstract

The application relates to the technical field of traditional Chinese medicine compositions, and particularly discloses a homologous composition of medicine and food containing giant salamander peptide for dispelling alcohol and protecting liver and a preparation method thereof. The homologous composition of medicine and food containing giant salamander peptide for dispelling alcohol and protecting liver contains giant salamander peptide and a traditional Chinese medicine composite extract, and the traditional Chinese medicine composite extract is prepared by extracting raw materials including green tea, turmeric, mulberry leaves, hawthorn, radix puerariae, hovenia dulcis thunb, poria cocos, semen cassiae, lotus leaf powder, amomum kravanh, chrysanthemum, kudzuvine flower and abrus precatorius by a double solvent system of alcohol extraction and water extraction. The application can effectively avoid the negative interaction between some traditional Chinese medicine components and the giant salamander peptide, guarantee the activity and absorption utilization rate, significantly enhance the efficacy, realize the dispelling alcohol and protecting liver functions close to the expected effect, and finally obtain the homologous composition of medicine and food containing giant salamander peptide for dispelling alcohol and protecting liver with better application quality.

Description

Technical Field

[0001] This application relates to the field of traditional Chinese medicine composition technology, and more specifically, it relates to a food-medicine homologous composition containing giant salamander peptide for relieving hangovers and protecting the liver, and its preparation method. Background Technology

[0002] With the fast pace of modern life, people often need to drink alcohol for social and business activities. However, excessive drinking not only damages the body but can also lead to a series of health problems, such as liver disease. Therefore, alcoholic liver disease has become a hidden killer that cannot be ignored in modern society, and the need for hangover relief and liver protection is receiving increasing attention.

[0003] Food and medicine homology products are diverse, consisting of substances that combine the properties of food and medicine, allowing them to better exert their nutritional value and medicinal effects; according to the wisdom of traditional Chinese medicine dietary therapy, these substances can exert their corresponding functions through daily dietary intake.

[0004] Giant salamander peptides have significant hepatoprotective and alcohol-relieving effects in food-medicine homologous products. They can activate the activity of alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH), accelerating the harmless metabolism of ethanol, acetaldehyde, and acetic acid, shortening the time alcohol remains in the body, and also protecting the liver from alcohol damage and maintaining normal liver function. Traditional Chinese medicines also have significant hepatoprotective and alcohol-relieving effects in food-medicine homologous products. For example, kudzu root, Japanese raisin tree fruit, and kudzu flower can significantly reduce blood ethanol concentration, increase ADH and ALDH activity in liver tissue, and improve liver function indicators, alleviating liver damage caused by ethanol by enhancing the body's antioxidant capacity. Existing technologies also aim to create more comprehensive food-medicine homologous solutions by synergistically combining giant salamander peptides with traditional Chinese medicine extracts.

[0005] Regarding the aforementioned technologies, the inventors believe that although the research on the synergistic effect of giant salamander peptides and traditional Chinese medicine extracts shows good application prospects, some traditional Chinese medicine components may react chemically with giant salamander peptides, leading to reduced activity and absorption and utilization, ultimately making it difficult to achieve the expected efficacy. Therefore, it is urgent to propose a solution to address the above-mentioned technical problems. Summary of the Invention

[0006] In order to enable the giant salamander peptide and traditional Chinese medicine ingredients to exert a stable and excellent synergistic effect in food and medicine homologous products, this application provides a food and medicine homologous composition containing giant salamander peptide for relieving hangovers and protecting the liver, and its preparation method.

[0007] In a first aspect, this application provides a food-grade composition containing giant salamander peptides for relieving hangovers and protecting the liver, employing the following technical solution:

[0008] A food-grade medicinal composition containing giant salamander peptide for relieving hangovers and protecting the liver, comprising giant salamander peptide and a compound extract of traditional Chinese medicine, wherein the compound extract of traditional Chinese medicine is prepared by extraction of the following raw materials in parts by weight using a dual solvent system of alcohol extraction and water extraction:

[0009] 280-320 servings of green tea;

[0010] 140-160 parts turmeric;

[0011] 180-220 portions of mulberry leaves;

[0012] 80-120 servings of hawthorn;

[0013] 90-110 portions of kudzu root;

[0014] 80-120 portions of Hovenia dulcis seeds;

[0015] Poria cocos 60-80 parts;

[0016] Cassia seeds 15-25 parts;

[0017] 15-25 parts lotus leaf powder;

[0018] 15-25 parts of cardamom;

[0019] Chrysanthemums 25-35 parts;

[0020] 80-120 portions of kudzu flower;

[0021] 60-80 servings of white hyacinth beans.

[0022] By adopting the above technical solution, the raw materials selected for the preparation of the traditional Chinese medicine compound extract include: kudzu root, kudzu flower, and Japanese raisin tree fruit, a classic combination for relieving hangovers and protecting the liver, which can promote alcohol metabolism and protect liver cells; turmeric, chrysanthemum, and cassia seed, which have antioxidant and anti-inflammatory effects and can reduce oxidative damage to the liver caused by alcohol; poria cocos, white hyacinth bean, hawthorn, and cardamom, which can strengthen the spleen and eliminate dampness, promote digestion, and help reduce the metabolic burden on the liver; and green tea, mulberry leaf, and lotus leaf powder, which can promote liver detoxification and metabolism. Through the careful combination of the above-mentioned traditional Chinese medicine components, a significant and excellent hangover-relieving and liver-protecting effect can be achieved. Moreover, the preparation of the traditional Chinese medicine compound extract uses dual solvent extraction, which not only separates components with different polarities but also fully extracts polysaccharides using water extraction and extracts fat-soluble active substances using alcohol extraction, ensuring the completeness of the medicinal components and the stability of the original activity of the traditional Chinese medicine compound extract. Meanwhile, the compatibility between the Chinese herbal components in the above-mentioned compound extract and the giant salamander peptide is good. The multi-target, multi-pathway, and multi-level interaction can effectively avoid some negative interactions between the Chinese herbal components and the giant salamander peptide, ensuring its activity and absorption rate, thereby significantly enhancing the efficacy and achieving a hangover relief and liver protection effect that is far close to the expectation. Finally, a high-quality medicinal and food homologous composition containing giant salamander peptide for hangover relief and liver protection is obtained.

[0023] Preferably, the mass percentage of the giant salamander peptide is 1-5%.

[0024] By adopting the above technical solution, the giant salamander peptide may not be able to exert its corresponding effect if it is less than 1%, while it may reduce the solubility due to peptide aggregation if it exceeds 5%. Therefore, the mass ratio of giant salamander peptide is 1-5%, which can meet the requirements for good and stable application. At this ratio, it can also avoid excessive binding with the Chinese medicine components in the Chinese medicine extract, which is conducive to ensuring compatibility stability. In this way, the combination of food and medicine containing giant salamander peptide for hangover relief and liver protection can exert excellent and stable efficacy.

[0025] Preferably, the herbal compound extract is prepared by extraction from raw materials comprising the following parts by weight using a dual solvent system of alcohol extraction and water extraction:

[0026] 300 servings of green tea;

[0027] 150 portions of turmeric;

[0028] 200 portions of mulberry leaves;

[0029] 100 servings of hawthorn;

[0030] 100 servings of kudzu root;

[0031] 100 portions of Japanese raisin tree seeds;

[0032] 70 parts of Poria cocos;

[0033] 20 portions of cassia seeds;

[0034] 20 portions of lotus leaf powder;

[0035] 20 portions of cardamom;

[0036] 30 portions of chrysanthemums;

[0037] 100 portions of kudzu flowers;

[0038] 70 servings of white hyacinth beans.

[0039] By adopting the above technical solution, the traditional Chinese medicine compound extract obtained by extracting the above-mentioned raw materials by weight has good compatibility with giant salamander peptides, and performs better in terms of the effects of relieving hangovers and protecting the liver.

[0040] Preferably, in the preparation of the traditional Chinese medicine compound extract, 75-85% ethanol is used in the alcohol extraction process, the material-to-liquid ratio is 1:(25-35), the extraction temperature is 65-75℃, the single extraction time is 55-65min, and the extraction is performed 2-3 times.

[0041] By adopting the above technical solution, 75-85% ethanol is very effective in extracting Chinese medicine components, while an extraction temperature of 65-75℃ can promote the dissolution of effective components and avoid the damage of heat-labile components caused by high temperature. At the same time, the material-liquid ratio of 1:(25-35) provides sufficient space for the full dissolution of effective components, and the extraction time of each extraction is controlled at 55-65min, which can ensure extraction efficiency and avoid introducing too many impurities due to excessive time. Finally, extraction 2-3 times can ensure that the effective components in the medicinal materials are fully extracted.

[0042] Preferably, in the preparation of the traditional Chinese medicine compound extract, the material-to-liquid ratio during water extraction is 1:(25-35), the extraction temperature is 95-100℃, the extraction time for a single extraction is 90-100 min, and the extraction is performed 2-3 times.

[0043] By adopting the above technical solution, a material-to-liquid ratio of 1:(25-35) provides sufficient diffusion space for water-soluble components such as polysaccharides and glycosides. Combined with an extraction temperature of 95-100℃ to disrupt the plant cell wall structure, the dissolution of effective components is significantly improved. At the same time, controlling the extraction time to 90-100 minutes per extraction ensures extraction efficiency without introducing too many impurities due to excessive time. Finally, extraction is performed 2-3 times to ensure that the effective components in the medicinal materials are fully extracted.

[0044] Preferably, the giant salamander peptide is coated before use, and the specific operation is as follows:

[0045] S1. Dissolve chitosan in glacial acetic acid solution and adjust the pH to 4-5 to obtain chitosan solution;

[0046] S2. Add giant salamander peptide to the chitosan solution obtained in step S1. Add sodium tripolyphosphate solution dropwise while stirring to adjust the pH value to 4-5. After stirring and reacting, filter and dry to obtain chitosan-coated giant salamander peptide material.

[0047] Phospholipid complexes are also added during the preparation of the traditional Chinese medicine compound extract.

[0048] By employing the above-mentioned technical solution, chitosan and sodium tripolyphosphate form a nanoscale composite membrane through electrostatic self-assembly, encapsulating the giant salamander peptide within. This physical barrier effectively prevents direct contact between the polyphenols, tannins, and other components in the traditional Chinese medicine extract and the giant salamander peptide, avoiding the inactivation or precipitation of active ingredients due to intermolecular forces. It also provides a sustained-release effect, further effectively mitigating potential negative interactions between the traditional Chinese medicine components in the composite extract and the giant salamander peptide. Furthermore, the use of a phospholipid complex in the preparation of the traditional Chinese medicine composite extract allows for the formation of a micellar structure, promoting the transmembrane transport of the giant salamander peptide. The phospholipid complex also acts as a regulatory bridge between the chitosan-encapsulated giant salamander peptide and the traditional Chinese medicine components in the composite extract, enabling them to exert a more synergistic effect. Meanwhile, the coating treatment of giant salamander peptide before use and the addition of phospholipid complex in the preparation of traditional Chinese medicine compound extracts can significantly reduce the potential negative interaction between traditional Chinese medicine components and giant salamander peptide through the dual mechanism of physical encapsulation and chemical compounding, thus significantly promoting its synergistic effect in relieving hangovers and protecting the liver.

[0049] Preferably, in the chitosan-coated giant salamander peptide material, the mass ratio of chitosan to giant salamander peptide is (1.2-1.5):1.

[0050] By adopting the above technical solution, this ratio range can ensure that chitosan molecules fully cover the surface of giant salamander peptides, forming a continuous and dense nanofilm layer. Excessive chitosan may lead to an excessively thick film layer, affecting the release of giant salamander peptides. Low chitosan content may easily result in uneven coating, leading to local exposure. At the same time, this ratio facilitates the construction of phospholipid complexes to regulate the bridge, thus achieving the dual goals of activity protection and functional synergy.

[0051] Preferably, the phospholipid complex accounts for 60-70% of the mass of the herbal compound extract.

[0052] By adopting the above technical solution, the synergistic effect of phospholipid complex and active ingredients of traditional Chinese medicine is fully realized at a ratio of 60-70%, which can effectively enhance the lipid solubility of poorly soluble active ingredients of traditional Chinese medicine and promote their binding to cell membranes, thereby significantly improving the absorption rate and bioavailability of drugs. At the same time, it can also play an excellent stabilizing role between traditional Chinese medicine components and giant salamander peptides, which is conducive to obtaining a high-quality alcohol-relieving and liver-protecting drug-food homologous composition containing giant salamander peptides.

[0053] Secondly, this application provides a method for preparing a food-derived composition containing giant salamander peptides for relieving hangovers and protecting the liver, using the following technical solution:

[0054] A method for preparing a food-grade medicinal composition containing giant salamander peptides for relieving hangovers and protecting the liver includes the following steps:

[0055] (1) Prepare the raw materials required for the preparation of giant salamander peptide and traditional Chinese medicine compound extract according to the proportion: green tea, turmeric, mulberry leaf, hawthorn, kudzu root, Japanese raisin tree fruit, poria cocos, cassia seed, lotus leaf powder, cardamom, chrysanthemum, kudzu flower and white hyacinth bean;

[0056] (2) Take green tea, turmeric, mulberry leaf, hawthorn, kudzu root, Japanese raisin tree fruit, poria cocos, cassia seed, lotus leaf powder, cardamom, chrysanthemum, kudzu flower and white hyacinth bean and mix them. Then, extract them with alcohol and water in sequence. Combine the extracts and filter them. Then, concentrate them under reduced pressure and spray dry them to obtain the Chinese herbal compound extract.

[0057] (3) After mixing the compound extract of traditional Chinese medicine and giant salamander peptide, a food-medicine composition containing giant salamander peptide for relieving hangovers and protecting the liver is obtained.

[0058] By adopting the above technical solution, the above preparation method is simple to operate and can obtain a high-quality and stable medicinal and edible composition containing giant salamander peptides for relieving hangovers and protecting the liver.

[0059] Preferably, a method for preparing a food-derived composition containing giant salamander peptides for relieving hangovers and protecting the liver includes the following steps:

[0060] (1) Prepare the raw materials required for the preparation of giant salamander peptide and traditional Chinese medicine compound extract, including green tea, turmeric, mulberry leaf, hawthorn, kudzu root, Japanese raisin tree fruit, poria cocos, cassia seed, lotus leaf powder, cardamom, chrysanthemum, kudzu flower, white hyacinth bean and phospholipid complex according to the formula ratio;

[0061] (2) Take green tea, turmeric, mulberry leaf, hawthorn, kudzu root, Japanese raisin tree fruit, poria cocos, cassia seed, lotus leaf powder, cardamom, chrysanthemum, kudzu flower and white hyacinth bean, mix them and extract them with alcohol and water in sequence, combine the resulting extracts and filter them, then add phospholipid complex and mix them, and then concentrate and spray dry under reduced pressure to obtain the Chinese herbal compound extract.

[0062] (3) After mixing the compound extract of traditional Chinese medicine and the chitosan-coated giant salamander peptide, a medicinal and food homologous composition containing giant salamander peptide for relieving hangovers and protecting the liver is obtained.

[0063] By adopting the above technical solutions, using chitosan to coat giant salamander peptides and adding phospholipid complexes to traditional Chinese medicine compound extracts, it is possible to obtain a food-medicine homologous composition containing giant salamander peptides for hangover relief and liver protection with better application quality.

[0064] In summary, this application has the following beneficial effects:

[0065] 1. This application carefully selects and combines various traditional Chinese medicine ingredients to significantly improve the compatibility and coordination between the traditional Chinese medicine ingredient system and the giant salamander peptide, thereby effectively avoiding the negative interactions that may occur between some traditional Chinese medicine ingredients and the giant salamander peptide, ensuring its activity and absorption rate, thus significantly enhancing the efficacy and achieving a hangover relief and liver protection effect that is far close to the expected effect, and finally obtaining a hangover relief and liver protection medicinal and food homologous composition containing giant salamander peptide with better application quality;

[0066] 2. This application involves coating the giant salamander peptide before use and adding a phospholipid complex in the preparation of the traditional Chinese medicine compound extract. This can significantly reduce the potential negative interaction between the traditional Chinese medicine components and the giant salamander peptide through a dual mechanism of physical encapsulation and chemical compounding, thereby significantly promoting its synergistic effect in relieving hangovers and protecting the liver. Attached Figure Description

[0067] Figure 1 This is a diagram showing the effect of a food-medicine composition containing giant salamander peptides, which is used for relieving hangovers and protecting the liver, on the behavior of drunk zebrafish.

[0068] Figure 2 The effect of a food-derived compound containing giant salamander peptides, which is used for hangover relief and liver protection, on fat production in drunken zebrafish. Detailed Implementation

[0069] The present application will be further described in detail below with reference to preparation examples, embodiments and comparative examples.

[0070] Unless otherwise specified, all raw materials used in the preparation examples, embodiments and comparative examples of this application are commercially available.

[0071] The giant salamander peptide was purchased from Xi'an Xihai Biotechnology Co., Ltd. as 98% water-soluble giant salamander peptide, product number XH647.

[0072] Chitosan was purchased from Shandong Huiheng Biotechnology Co., Ltd., and the average mass of chitosan molecules is 50 kDa.

[0073] The phospholipid complex was soybean lecithin, purchased from Wuhan Kemic Biomedical Technology Co., Ltd., CAS number 8002-43-5.

[0074] Preparation examples of raw materials and / or intermediates

[0075] Preparation Example 1

[0076] A chitosan-coated giant salamander peptide material is obtained by coating the giant salamander peptide before use. The specific operation is as follows:

[0077] S1. Dissolve chitosan in a 1% glacial acetic acid solution and adjust the pH to 4-5 to obtain a chitosan solution with a concentration of 0.5 mg / mL.

[0078] S2. Add giant salamander peptide to the chitosan solution obtained in step S1. During stirring, add sodium tripolyphosphate solution with a concentration of 0.8 mg / mL dropwise to adjust the pH value to 4-5. Then, stir and react at 25°C for 30 min. After filtration and drying, obtain chitosan-coated giant salamander peptide material.

[0079] Note: The preferred pH value in operations S1 and S2 is 4; the mass ratio of chitosan to sodium tripolyphosphate is 5:1; and the mass ratio of chitosan to giant salamander peptide is 1.35:1.

[0080] Preparation Example 2

[0081] A chitosan-coated giant salamander peptide material differs from Preparation Example 1 in that the mass ratio of chitosan to giant salamander peptide is 1.2:1.

[0082] Preparation Example 3

[0083] A chitosan-coated giant salamander peptide material differs from Preparation Example 1 in that the mass ratio of chitosan to giant salamander peptide is 1.5:1.

[0084] Example

[0085] Example 1

[0086] A medicinal and edible composition containing giant salamander peptide for relieving hangovers and protecting the liver is composed of giant salamander peptide and a compound extract of traditional Chinese medicine. The compound extract of traditional Chinese medicine is prepared by a dual solvent system of alcohol extraction and water extraction. The raw materials used in the preparation and their corresponding weight parts are shown in Table 1. The preparation is carried out through the following steps:

[0087] (1) Prepare the raw materials required for the preparation of giant salamander peptide and traditional Chinese medicine compound extract according to the proportion: green tea, turmeric, mulberry leaf, hawthorn, kudzu root, Japanese raisin tree fruit, poria cocos, cassia seed, lotus leaf powder, cardamom, chrysanthemum, kudzu flower and white hyacinth bean;

[0088] (2) Take green tea, turmeric, mulberry leaf, hawthorn, kudzu root, Japanese raisin tree fruit, poria cocos, cassia seed, lotus leaf powder, cardamom, chrysanthemum, kudzu flower and white hyacinth bean and mix them. Then, extract them with alcohol and water in sequence. Combine the extracts and filter them. Then, concentrate them under reduced pressure and spray dry them to obtain the Chinese herbal compound extract.

[0089] (3) After mixing the compound extract of traditional Chinese medicine and giant salamander peptide, a food-medicine composition containing giant salamander peptide for relieving hangovers and protecting the liver is obtained.

[0090] Note: In the above operations, 80% ethanol was used for alcohol extraction, with a solid-liquid ratio of 1:30, an extraction temperature of 70℃, a single extraction time of 60 min, and two extractions were performed. For water extraction, the solid-liquid ratio was 1:30, the extraction temperature was 100℃, a single extraction time of 95 min, and two extractions were performed. The mass percentage of giant salamander peptide was 3%.

[0091] Example 2-3

[0092] A medicinal and edible composition containing giant salamander peptides for relieving hangovers and protecting the liver differs from Example 1 in that the raw materials used in the preparation of the compound extract of traditional Chinese medicine and their corresponding weight parts are shown in Table 1.

[0093] Table 1. Raw materials used in the preparation of compound extracts of traditional Chinese medicine in Examples 1-3 and their corresponding weight parts (parts / kg)

[0094]

[0095] Example 4

[0096] A food-derived composition containing giant salamander peptides for relieving hangovers and protecting the liver, differs from Example 1 in that 75% ethanol is used in the alcohol extraction process, the material-to-liquid ratio is 1:35, the extraction temperature is 75℃, the single extraction time is 55min, and the extraction is performed 3 times.

[0097] Example 5

[0098] A food-derived composition containing giant salamander peptide for relieving hangovers and protecting the liver, differs from Example 1 in that 85% ethanol is used in the alcohol extraction process, the material-to-liquid ratio is 1:25, the extraction temperature is 65℃, the single extraction time is 65min, and the extraction is performed twice.

[0099] Example 6

[0100] A food-derived composition containing giant salamander peptides for relieving hangovers and protecting the liver, differs from Example 1 in that the material-to-liquid ratio during water extraction is 1:25, the extraction temperature is 95℃, the single extraction time is 100min, and the extraction is performed 3 times.

[0101] Example 7

[0102] A food-derived composition containing giant salamander peptides for relieving hangovers and protecting the liver, differs from Example 1 in that the material-to-liquid ratio during water extraction is 1:35, the extraction temperature is 97.5℃, the single extraction time is 90 min, and the extraction is performed 3 times.

[0103] Example 8

[0104] A food-derived composition containing giant salamander peptide for relieving hangovers and protecting the liver, differing from Example 1 in that the giant salamander peptide accounts for 1% of the total mass.

[0105] Example 9

[0106] A medicinal and edible composition containing giant salamander peptide for relieving hangovers and protecting the liver, differing from Example 1 in that the giant salamander peptide accounts for 5% of the total mass.

[0107] Example 10

[0108] A food-grade composition containing giant salamander peptides for relieving hangovers and protecting the liver, differing from Example 1 in that it is prepared through the following steps:

[0109] (1) Prepare the raw materials required for the preparation of giant salamander peptide and traditional Chinese medicine compound extract, including green tea, turmeric, mulberry leaf, hawthorn, kudzu root, Japanese raisin tree fruit, poria cocos, cassia seed, lotus leaf powder, cardamom, chrysanthemum, kudzu flower, white hyacinth bean and phospholipid complex according to the formula ratio;

[0110] (2) Take green tea, turmeric, mulberry leaf, hawthorn, kudzu root, Japanese raisin tree fruit, poria cocos, cassia seed, lotus leaf powder, cardamom, chrysanthemum, kudzu flower and white hyacinth bean, mix them and extract them with alcohol and water in sequence, combine the resulting extracts and filter them, then add phospholipid complex and mix them, and then concentrate and spray dry under reduced pressure to obtain the Chinese herbal compound extract.

[0111] (3) After mixing the compound extract of traditional Chinese medicine and the chitosan-coated giant salamander peptide, a medicinal and food homologous composition containing giant salamander peptide for relieving hangovers and protecting the liver is obtained.

[0112] Note: In the above operations, the chitosan-coated giant salamander peptide material was obtained from Preparation Example 1; in the traditional Chinese medicine compound extract, the phospholipid complex accounts for 65% of the mass.

[0113] Example 11

[0114] A food-derived composition containing giant salamander peptides for relieving hangovers and protecting the liver, differing from Example 10 in that the chitosan-coated giant salamander peptide material was obtained from Preparation Example 2.

[0115] Example 12

[0116] A food-derived composition containing giant salamander peptides for relieving hangovers and protecting the liver, differing from Example 10 in that the chitosan-coated giant salamander peptide material was obtained from Preparation Example 3.

[0117] Example 13

[0118] A medicinal and edible composition containing giant salamander peptides for relieving hangovers and protecting the liver, differs from Example 10 in that the phospholipid complex accounts for 60% of the mass of the traditional Chinese medicine compound extract.

[0119] Example 14

[0120] A medicinal and edible composition containing giant salamander peptides for relieving hangovers and protecting the liver, differs from Example 10 in that the phospholipid complex accounts for 70% of the mass of the traditional Chinese medicine compound extract.

[0121] Example 15

[0122] A medicinal and edible composition containing giant salamander peptides for relieving hangovers and protecting the liver, differing from Example 10 in that the chitosan-coated giant salamander peptide material is replaced with giant salamander peptide raw material.

[0123] Example 16

[0124] A medicinal and edible composition containing giant salamander peptides for relieving hangovers and protecting the liver, differs from Example 10 in that the phospholipid complex is not used in the herbal compound extract.

[0125] Comparative Example

[0126] Comparative Example 1

[0127] A medicinal and edible composition for relieving hangovers and protecting the liver, which differs from Example 1 in that it does not use giant salamander peptides in the raw materials.

[0128] Comparative Example 2

[0129] A medicinal and edible composition for relieving hangovers and protecting the liver, which differs from Example 1 in that no compound extracts of traditional Chinese medicine are used in the raw materials.

[0130] Performance testing

[0131] Test samples: The alcohol-relieving and liver-protecting medicinal and food homologous compositions containing giant salamander peptides obtained in Examples 1-16 were selected as test samples 1-16, and the alcohol-relieving and liver-protecting medicinal and food homologous compositions obtained in Comparative Examples 1-2 were selected as control samples 1-2.

[0132] Experimental Methods: 190 mice, half male and half female, were randomly divided into 19 groups of 10 mice each, with 5 males and 5 females per group. 18 groups were experimental groups, corresponding to test samples 1-16 and control samples 1-2, respectively. One group served as a blank control group. The following tests were then conducted:

[0133] In the experimental group, mice were fasted for 12 hours before the test. During the experiment, mice were first given a single dose of 53% ethanol by gavage (0.30 mL / 20 g). After 20 minutes, blood was drawn to measure the plasma ethanol concentration, which was recorded as the initial value A0. Then, mice were given a single dose of a hepatotoxic and liver-protecting combination containing giant salamander peptides by gavage (40 mg / kg). After 3 hours, blood was drawn to measure the plasma ethanol concentration, which was recorded as the termination value A1. The reduction rate of plasma ethanol concentration was then calculated as (A0 - A1) / A0. The average reduction rate of plasma ethanol concentration was recorded as the average reduction rate of plasma ethanol concentration for a group of 10 mice. The higher the average reduction rate of plasma ethanol concentration, the stronger the efficacy of the hepatotoxic and liver-protecting combination.

[0134] In the blank control group, mice were fasted for 12 hours before the test. During the experiment, mice were first given a single gavage of 0.30 mL / 20 g of 53% ethanol. After 20 minutes, blood was drawn to measure the plasma ethanol concentration, which was recorded as the initial value A0. After 3 hours, blood was drawn to measure the plasma ethanol concentration, which was recorded as the termination value A1. The decrease rate of plasma ethanol concentration was then calculated as (A0 - A1) / A0. The average value of 10 mice in a group was recorded as the average decrease rate of plasma ethanol concentration.

[0135] After performing the above tests on test samples 1-16 and control samples 1-2, the test results are recorded in Table 2.

[0136] Table 2 Test results of test samples 1-16 and control samples 1-2

[0137]

[0138] As can be seen from Example 1, Comparative Examples 1-2, the blank control group, and Table 2, this application significantly improves the compatibility and coordination between the selected traditional Chinese medicine components and the giant salamander peptide by carefully combining multiple traditional Chinese medicine components. Although the giant salamander peptide or the traditional Chinese medicine compound extract can bring corresponding effects on the basis of the blank control group when used alone, the sum of the improvement effects brought by the individual application is far less than that of the combination of the two. Thus, the combination of the two can bring about a significant enhancement of the corresponding efficacy.

[0139] As can be seen from Examples 1 and 10-14, and Table 2, the coating treatment of giant salamander peptides before use and the addition of phospholipid complexes in the preparation of traditional Chinese medicine compound extracts in this application can significantly enhance the corresponding efficacy through a dual mechanism of physical encapsulation and chemical compounding. Furthermore, as can be seen from Examples 15-16 and Table 2, while coating giant salamander peptides before use or adding phospholipid complexes only in the preparation of traditional Chinese medicine compound extracts can enhance the corresponding effects, the enhancement is limited. Moreover, based on the above experimental data, the combination of the two can achieve a significant improvement effect of 1+1>2.

[0140] at the same time, Figure 1 and Figure 2 All of these were based on the alcohol-relieving and liver-protecting medicinal and food homologous composition containing giant salamander peptides obtained in Example 1; from Figure 1 The study directly demonstrated that the alcohol-relieving and liver-protecting composition containing giant salamander peptides can improve the behavioral damage caused by alcohol to the central nervous system, and restore the zebrafish's motor, exploratory and social abilities. Figure 2 Histopathological evidence demonstrates that anti-alcohol drugs have a protective effect on the liver, reducing alcoholic liver damage and fatty degeneration; therefore, Figure 1 and Figure 2 The combination of these factors proves that this food-grade composition containing giant salamander peptides has both hangover-relieving and liver-protecting effects.

[0141] This specific embodiment is merely an explanation of this application and is not intended to limit it. After reading this specification, those skilled in the art can make modifications to this embodiment without contributing any inventive step, but such modifications are protected by patent law as long as they fall within the scope of the claims of this application.

Claims

1. An andro medon peps id e-containing food-herb composition for protecting liver and relieving alcoholism, characterized in that, The application relates to a hangover-preventing and liver-protecting medicine-food homologous composition containing andei peptides and a traditional Chinese medicine compound extract. Green tea 280-320 parts; Turmeric 140-160 parts; Mulberry leaf 180-220 parts; Hawthorn 80-120 parts; Radix Puerariae 90-110 parts; Hovenia dulcis 80-120 parts; Poria cocos 60-80 parts; Cassia seed 15-25 parts; Lotus leaf powder 15-25 parts; Amomum kravanh 15-25 parts; Chrysanthemum 25-35 parts; Kudzu flower 80-120 parts; Lablab 60-80 parts; The andei peptides are coated before use, and the specific operation is as follows: S1, chitosan is dissolved in glacial acetic acid solution, and the pH value is adjusted to 4-5 to obtain a chitosan solution; S2, andei peptides are added into the chitosan solution obtained in step S1, sodium tripolyphosphate solution is added dropwise during stirring, the pH value is adjusted to 4-5, and then stirring reaction is carried out, and the chitosan-coated andei peptide material is obtained after filtration and drying; Phospholipid compound is further added during preparation of the traditional Chinese medicine compound extract; In the chitosan-coated andei peptide material, the mass ratio of chitosan to andei peptides is (1.2-1.5):1; In the traditional Chinese medicine compound extract, the mass percentage of the phospholipid compound is 60-70%.

2. The Andrias davidianus peptide-containing alcohol-protecting and liver-protecting food-herbal composition according to claim 1, characterized in that: The mass percentage of the andei peptides is 1-5%.

3. The Andrias davidianus peptide-containing alcohol-protecting and liver-protecting food-herbal composition according to claim 1, characterized in that: The traditional Chinese medicine compound extract is prepared from raw materials containing the following components by alcohol extraction and water extraction of a double-solvent system: Green tea 300 parts; Turmeric 150 parts; Mulberry leaf 200 parts; Hawthorn 100 parts; Radix Puerariae 100 parts; Hovenia dulcis 100 parts; Poria cocos 70 parts; Cassia seed 20 parts; Lotus leaf powder 20 parts; Amomum kravanh 20 parts; Chrysanthemum 30 parts; Kudzu flower 100 parts; Lablab 70 parts.

4. The Androcambs chinensis peptide-containing alcohol-protecting and liver-protecting food-herbal composition according to claim 1, characterized in that: During preparation of the traditional Chinese medicine compound extract, 75-85% ethanol is used in the alcohol extraction process, the solid-liquid ratio is 1:(25-35), the extraction temperature is 65-75 DEG C, the single extraction time is 55-65 min, and the extraction is carried out 2-3 times.

5. The Andro Mediated Liver-Protecting and Anti-Alcoholic Food-Drug Composition of Claim 1, wherein: During preparation of the traditional Chinese medicine compound extract, the solid-liquid ratio is 1:(25-35) in the water extraction process, the extraction temperature is 95-100 DEG C, the single extraction time is 90-100 min, and the extraction is carried out 2-3 times.

6. The method for preparing the Andrias davidianus peptide-containing alcohol-protecting and liver-protecting food-herbal composition of claim 1, characterized in that: The method comprises the following steps: (1) preparing green tea, turmeric, mulberry leaf, hawthorn, radix puerariae, hovenia dulcis, poria cocos, cassia seed, lotus leaf powder, amomum kravanh, chrysanthemum, kudzu flower, lablab and phospholipid compound according to the proportion required for preparation of andei peptides and the traditional Chinese medicine compound extract; (2) mixing green tea, turmeric, mulberry leaf, hawthorn, radix puerariae, hovenia dulcis, poria cocos, cassia seed, lotus leaf powder, amomum kravanh, chrysanthemum, kudzu flower and lablab, and then sequentially carrying out alcohol extraction and water extraction, combining the obtained extraction liquid, filtering, then adding the phospholipid compound, mixing, and then carrying out vacuum concentration and spray drying to obtain the traditional Chinese medicine compound extract; (3) mixing the traditional Chinese medicine compound extract and the chitosan-coated andei peptide material to obtain the hangover-preventing and liver-protecting medicine-food homologous composition containing andei peptides.

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