A natural ecological bacteria cultivation device for saline-alkali soil improvement

Abstract

The application discloses a natural ecological strain cultivation device for saline-alkali soil improvement, and belongs to the technical field of strain cultivation. The natural ecological strain cultivation device for saline-alkali soil improvement comprises a supporting bottom bin, a plurality of anti-deposition liquid storage assemblies are slidably connected to one side of the supporting bottom bin, a sealed cabin is fixedly connected to the top end of the supporting bottom bin, a plurality of multilayer cabin assemblies are fixedly connected to the two sides of the sealed cabin, an irradiation frame is rotatably connected to the inner side of the sealed cabin, an isolation assembly is fixedly connected to the middle of the inner side of the multilayer cabin assembly, and a rotating cabinet is rotatably connected to the outer side of the isolation assembly. The application simulates the day and night temperature difference in the nature through the partition of the cold cabin and the hot cabin, and synchronously links the irradiation frame and the rotating cabinet, and the synergistic change cooperates with the spraying assembly, so that the real physical stress and recovery cycle of the saline-alkali soil strain in the natural environment are highly restored, and thus the target strain with stronger adaptability and more stable improvement effect can be more efficiently screened and cultivated.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of strain cultivation, and particularly relates to a natural ecological strain cultivation device for saline-alkali soil improvement. BACKGROUND

[0002] The natural ecological strain for saline-alkali soil improvement refers to indigenous microorganisms including bacteria and fungi, which are isolated and screened from natural environments such as saline-alkali soil or salt-tolerant plant roots and have salt-tolerant characteristics. After being cultured and expanded, the strain is applied to saline-alkali soil to improve the physicochemical properties of the soil, enhance the fertility of the soil and promote the growth of plants, so as to achieve ecological restoration. This is currently the most safe, economic and efficient biological improvement measure.

[0003] However, when such a strain is cultivated and functionally studied in a laboratory on a large scale, it faces special challenges. The growth environment needs to simulate the specific conditions of saline-alkali soil, such as salt stress, while providing suitable temperature, humidity, light and nutrients. The general microbial incubator or fermenter in the prior art often has a single function, and its environmental simulation capability is insufficient, which cannot effectively simulate the variable environment required by the strain from adaptation to expansion. SUMMARY

[0004] The technical problem to be solved by the application is to overcome the shortcomings of the prior art and provide a natural ecological strain cultivation device for saline-alkali soil improvement.

[0005] The technical solution adopted to solve the above technical problem is as follows: a natural ecological strain cultivation device for saline-alkali soil improvement, comprising a support bottom bin, a plurality of anti-settling liquid storage assemblies are connected to one side of the support bottom bin in a sliding manner, a sealed cabin is fixedly connected to the top end of the support bottom bin, a plurality of multilayer cabin assemblies are fixedly connected to the two sides of the sealed cabin, a monitoring panel is arranged on the surface of the sealed cabin, an irradiation frame is rotatably connected to the inner side of the sealed cabin, an isolation assembly is fixedly connected to the middle of the inner side of the multilayer cabin assembly, a rotating cabinet is rotatably connected to the outer side of the isolation assembly, a plurality of culture media are slidably connected in the rotating cabinet, and the rotating cabinet and the irradiation frame are rotatably connected to each other.

[0006] Further, two isolation assemblies are fixedly connected to the inner side of the isolation assembly, and a spraying assembly is fixedly connected to the inner side of the isolation assembly.

[0007] Further, the culture medium is provided with a high-density gauze at the bottom end, the culture medium is located between the spraying assemblies, and the spraying assemblies are fixedly connected to the anti-settling liquid storage assemblies.

[0008] Through the above technical solution, the high-density gauze is arranged to allow the salt solution at the spraying end to slowly penetrate upward, thereby simulating the salt environment in saline-alkali soil, and the arrangement of the anti-settling liquid storage assembly ensures the accuracy of the liquid concentration.

[0009] Further, the isolation assembly comprises a stand column, the bottom end of the stand column is fixedly connected with the support bottom bin, the middle part of the stand column is fixedly connected with an annular hopper, the two sides of the annular hopper are fixedly connected with the air inlet assembly respectively, the top end of the annular hopper is fixedly connected with T-shaped isolation plates on the two sides and the inner side of the stand column respectively, the outer side of the T-shaped isolation plates is fixedly connected with the spraying assembly, the two sides of the stand column are divided into a cold cabin and a hot cabin by the T-shaped isolation plates, the outer wall of the T-shaped isolation plates is rotationally attached to the inner wall of the rotary cabinet, and the cold cabin and the hot cabin are in communication with the inside of the rotary cabinet respectively.

[0010] Through the above technical scheme, the T-shaped isolation plates and the stand column divide the annular space of the rotary cabinet into two halves, forming independent cold cabin and hot cabin for partition temperature control, ensuring that the airflow of two different temperatures can be strictly limited in the respective areas, minimizing the mixing of cold and hot air, providing a foundation for creating a clear and stable temperature gradient. When the culture medium rotates with the rotary cabinet, it can periodically and sequentially pass through the cold cabin and the hot cabin, thereby simulating the day-night alternating environment, which is more suitable for the natural growth environment of the strain, and improving the survival rate of the strain after transplanting.

[0011] Further, the rotary cabinet is provided with a plurality of compartments, a plurality of said compartments on the same layer are rotationally corresponding to a plurality of irradiation lamps, a plurality of said compartments are respectively installed with a blocking strip on the two sides, the top end of the blocking strip is slidingly connected with the culture medium, the top end of the rotary cabinet is fixedly connected with a single spool, and the single spool is respectively frictionally connected with two belts.

[0012] Further, the irradiation frame comprises a rotating rod, the bottom end of the rotating rod is rotationally connected with a second motor, the power output end of the second motor is fixedly connected with the support bottom bin, a plurality of six-paw turntables are fixedly connected in an up-down array on the middle part of the rotating rod, a plurality of irradiation lamps are arranged on the bottom end of the six-paw turntables, a double-groove spool is fixedly connected to the top end of the rotating rod, and two belts are frictionally connected to the surface of the double-groove spool respectively.

[0013] Through the above technical scheme, the motor drives the irradiation frame and the rotary cabinet to rotate synchronously through the spool belt structure, and the interlaced rotation makes the irradiation lamps simulate the sun's irradiation angle. The six-paw turntable on the irradiation frame rotates by a certain angle, thereby changing the incident angle of light or simulating the photoperiod change of sunrise and sunset, simulating the real day-night cycle and alternating environment of saline-alkali land, thereby efficiently screening and cultivating adaptive strains.

[0014] Further, the spraying assembly comprises a plurality of annular sleeves, a sealing annular plate is rotatably connected to the outside of the annular sleeve, an extension pipe is mounted on the surface of the sealing annular plate, a nozzle is arranged at the end of the extension pipe away from the sealing annular plate, a plurality of annular sleeves are arranged on the upper and lower ends of the inner side of each compartment, the two nozzles in the same compartment are oppositely arranged, a plurality of annular sleeves on the top end of the inner side of the compartment are fixedly connected to the inner side of the upper connecting pipe, and a plurality of annular sleeves on the bottom end of the inner side of the compartment are fixedly connected to the inner side of the lower connecting pipe. The lower connecting pipe and the upper connecting pipe are fixedly connected with the anti-deposition liquid storage assembly.

[0015] Through the above technical scheme, the annular sleeve and the sealing annular plate cooperate with each other, so that even if the rotating cabinet drives the culture medium to rotate continuously, the nozzle can still maintain the basic stability of its spatial orientation through the relative rotation of the sealing annular plate, ensuring that the spraying direction always points to the culture area. The upper and lower nozzles create a three-dimensional spraying effect. The lower nozzle can simulate the direction and intensity of salt stress, and the upper nozzle simulates the irrigation effect in the real environment. This not only realizes fine adjustment of the spraying intensity and mode, but also simulates the growth environment of the fungus in the real environment, further improving the fungus cultivation effect.

[0016] Further, the air inlet assembly comprises a first pipeline and a second pipeline, the second pipeline is fixedly communicated with the cold cabin, a circulating air duct is fixedly connected to the middle of the second pipeline, a semiconductor heat sink is fixedly connected to the inside of the circulating air duct, one end of the semiconductor heat sink is fixedly connected with the second pipeline, the circulating air duct is fixedly connected with the supporting bottom bin, a first electric fan is installed on the penetrating end of the circulating air duct, the first pipeline is fixedly communicated with the hot cabin, the middle of the second pipeline and the middle of the first pipeline are fixedly connected with an air inlet duct, a second electric fan is fixedly connected to the inside of the air inlet duct, the air inlet duct is fixedly connected with the supporting bottom bin, and a filter screen is installed on the penetrating end of the air inlet duct.

[0017] Through the above technical scheme, the air inlet duct delivers the filtered air into the first pipeline and the second pipeline, and the circulating air duct cools the semiconductor heat sink through the flowing air, thereby cooling the air in the second pipeline, achieving the cooling effect of the cold cabin, simulating the night cooling environment. After the air in the first pipeline enters the hot cabin, it is heated by the irradiation lamp, simulating the daytime warming environment, and achieving precise simulation of day and night alternation.

[0018] Further, the anti-precipitation liquid storage assembly comprises a pull plate, the inner side of the pull plate is fixedly connected with a stand, the bottom ends of the two sides of the stand are slidably connected with fixed carriages, the fixed carriages are fixedly connected with the supporting bottom bin, the top end of the side close to the pull plate of the stand is rotatably connected with a fixed frame, the inner side of the fixed frame is slidably connected with square buckets, the inner parts of the square buckets are respectively filled with clean water, brine and nutrient solution, the bottom end of the side away from the pull plate of the fixed frame is fixedly connected with an extension plate, one end of the extension plate is slidably connected with a roller, the roller is slidably connected with the fixed carriages in penetration, the penetration end of the roller is rotatably connected with a push handle, the bottom end of the side of the fixed carriage is fixedly connected with a plurality of mounting sleeves, the mounting sleeves are rotatably connected with a transmission rod in penetration, a plurality of cams are fixedly connected with the middle part of the transmission rod, the surface of the cam is slidably attached to the surface of the push handle, the one end of the transmission rod is rotatably connected with a first motor, the power output end of the first motor is fixedly connected with the transmission rod, the first motor is fixedly connected with the fixed carriage, the top end of the side close to the fixed carriage of the stand is fixedly connected with a pump, the water inlet of the pump is located at the inner bottom end of the square bucket, a mixing tank is fixedly connected between the water outlet of the pump connected with the square bucket filled with clean water and nutrient solution and the lower connecting pipe.

[0019] Through the above technical scheme, the motor drives the cam to rotate, when the roller is attached to the cam, the push handle continuously pushes the square bucket at the bottom to shake, the low-amplitude and high-frequency shaking can effectively prevent the solute from settling, ensures that the concentration of the solution in the barrel is uniform at all times, is conducive to long-term cultivation, reduces manual operation, the system can instruct the mixing tank to pump the nutrient solution for spraying, and can also pump the brine for stress, the change of the chemical environment is a simulation of the real saline-alkali ecological condition, and the effectiveness of the strain cultivation is ensured.

[0020] Further, the multi-layer cabin assembly comprises a semicircular sleeve, the semicircular sleeve is fixedly connected with a sealed cabin, the inner wall of the sealed cabin is rotatably attached to a rotating cabinet, the middle part of the semicircular sleeve is slidably connected with a sealing door, the middle part of the sealing door is provided with an observation window, one side of the surface of the observation window is rotatably connected with a light shield, and the outer surface of the semicircular sleeve is slidably connected with an arc-shaped door.

[0021] Through the above technical scheme, a double-layer safety structure is formed, and the rotating rotating cabinet enables each compartment to be individually observed and accessed, reduces the damage to the internal environment caused by observation and access operations, and ensures the stability of the internal environment.

[0022] The beneficial effects of the present application are as follows:

[0023] The application integrates the spraying assembly in depth by cooperating the isolation assembly with the air inlet assembly, the rotating cabinet and the irradiation frame, and builds a cultivation core capable of accurately simulating a dynamic complex environment. The partition of the cold cabin and the hot cabin, combined with the periodic rotation of the rotating cabinet, makes the culture medium automatically experience programmed temperature cycle, simulating the day and night and seasonal temperature difference in nature; at the same time, the synchronous linkage of the irradiation frame and the rotating cabinet realizes the simulation of the light angle and period. The synergistic change of temperature and light in space and time highly restores the real physical stress and recovery cycle of the saline-alkali soil bacteria in the natural environment, so that the target bacteria with stronger adaptability and more stable improvement effect can be more efficiently screened and cultivated.

[0024] 2. The application significantly improves the cultivation efficiency and operation convenience through highly automated and modularized design. From the anti-settling storage of liquid, accurate pumping, to the temperature control cycle of environmental gas, the periodic change of light, to the uniform rotation of the culture unit, the whole process can be controlled by the monitoring panel to realize automatic operation of the whole process, and the manual intervention and pollution risk are minimized. At the same time, the observation window and the sealing door of the multi-layer cabin assembly facilitate non-destructive observation and sampling; the drawer type design of the anti-settling liquid storage assembly is convenient to maintain; the modularized pulling and replacement of the culture medium is simple and fast, so that the device is not only suitable for rigorous laboratory research, but also has the potential to be applied to large-scale and standardized production. BRIEF DESCRIPTION OF DRAWINGS

[0025] Figure 1 is the first structural schematic diagram of the application;

[0026] Figure 2 is the second structural schematic diagram of the application;

[0027] Figure 3 is the internal structure perspective schematic diagram of the application;

[0028] Figure 4 is the overall internal structure cross-sectional schematic diagram of the application;

[0029] Figure 5 is the installation structure perspective schematic diagram of the isolation assembly and the air inlet assembly of the application;

[0030] Figure 6 is the structural front view schematic diagram of the rotating cabinet of the application;

[0031] Figure 7 is the installation structure perspective schematic diagram of the spraying assembly of the application;

[0032] Figure 8 is the local structure perspective schematic diagram of the spraying assembly of the application;

[0033] Figure 9 is the air inlet duct structure perspective schematic diagram of the application;

[0034] Figure 10 is the medium structure of the present application

[0035] Figure 11 is the overall structure of the anti-settling liquid storage assembly of the present application

[0036] Figure 12 is the partial structure of the anti-settling liquid storage assembly of the present application.

[0037] Figure 1, support bottom bin; 2, anti-settling liquid storage assembly; 201, pull plate; 202, square bucket; 203, fixed frame; 204, stand; 205, fixed carriage; 206, pump; 207, mixing tank; 208, transmission rod; 209, mounting sleeve; 210, cam; 211, first motor; 212, extension plate; 213, push handle; 214, roller; 3, sealed cabin; 4, monitoring panel; 5, multi-layer cabin assembly; 501, semicircular sleeve; 502, arc-shaped door; 503, sealed door; 504, light shield; 505, observation window; 6, rotating cabinet; 601, compartment; 602, blocking bar; 603, single spool; 7, culture medium; 701, high-density gauze; 8, isolation assembly; 801, upright column; 802, T-shaped isolation plate; 803, cold cabin; 804, hot cabin; 805, annular hopper; 9, irradiation rack; 901, rotating rod; 902, second motor; 903, six-claw turntable; 904, irradiation lamp; 905, double-groove spool; 906, belt; 10, air inlet assembly; 1001, first pipeline; 1002, second pipeline; 1003, circulating air duct; 1004, air inlet air duct; 1005, first electric fan; 1006, filter screen; 1007, second electric fan; 1008, semiconductor heat sink; 11, spraying assembly; 1101, annular sleeve; 1102, sealed annular plate; 1103, extension pipe; 1104, spout; 1105, lower connecting pipe; 1106, upper connecting pipe. DETAILED DESCRIPTION

[0038] In order to make the purpose, technical scheme and advantages of the present application more clear, the present application is further described in detail below in combination with the drawings and examples. It should be understood that the specific examples described herein are only used to explain the present application and do not limit the present application.

[0039] As Figures 1 to 12As shown, the natural ecological strain cultivation device for saline-alkali soil improvement of the embodiment comprises a support bottom bin 1, a plurality of anti-settling liquid storage assemblies 2 are connected to one side of the support bottom bin 1 in a sliding manner, a sealed cabin 3 is fixedly connected to the top end of the support bottom bin 1, a plurality of multilayer cabin assemblies 5 are fixedly connected to the two sides of the sealed cabin 3, a monitoring panel 4 is arranged on the surface of the sealed cabin 3, an irradiation frame 9 is rotatably connected to the inner side of the sealed cabin 3, an isolation assembly 8 is fixedly connected to the middle of the inner side of the multilayer cabin assembly 5, a rotating cabinet 6 is rotatably connected to the outer side of the isolation assembly 8, and a plurality of culture media 7 are slidably connected inside the rotating cabinet 6. The rotating cabinet 6 and the irradiation frame 9 are rotatably connected to each other.

[0040] As shown in the Figures 1 to 2 multilayer cabin assembly 5 comprises a semicircular sleeve 501, the semicircular sleeve 501 is fixedly connected to the sealed cabin 3, the inner wall of the sealed cabin 3 is rotatably attached to the rotating cabinet 6, a sealing door 503 is slidably connected to the middle of the semicircular sleeve 501 in an up-down uniform manner, an observation window 505 is arranged in the middle of the sealing door 503, a light shield 504 is rotatably connected to one side of the surface of the observation window 505, and an arc-shaped door 502 is slidably connected to the outer surface of the semicircular sleeve 501.

[0041] As shown in the Figures 3 to 4 irradiation frame 9 comprises a rotating rod 901, a second motor 902 is rotatably connected to the bottom end of the rotating rod 901, the power output end of the second motor 902 is fixedly connected to the support bottom bin 1, a plurality of six-paw turntables 903 are fixedly connected to the middle of the rotating rod 901 in an up-down arrayed manner, a plurality of irradiation lamps 904 are arranged at the bottom end of the six-paw turntables 903, in the rotating process, the change of the sunlight irradiation angle is simulated, and the irradiation dead angle is also reduced, a double-groove spool 905 is fixedly connected to the top end of the rotating rod 901, two belts 906 are frictionally connected to the surface of the double-groove spool 905 respectively, the two belts 906 are frictionally connected to the single spool 603 respectively, and the two belts 906 move synchronously, which simplifies the transmission structure, reduces the cost and failure rate.

[0042] As shown in the Figures 2 to 4As shown, the two isolation assemblies 8 are fixedly connected with the spraying assemblies 11 on the inner side, and the bottom ends of the two isolation assemblies 8 are fixedly connected with the air inlet assembly 10, the isolation assembly 8 comprises a stand column 801, the bottom end of the stand column 801 is fixedly connected with the supporting bottom bin 1, the middle part of the stand column 801 is fixedly connected with an annular hopper 805, the two sides of the annular hopper 805 are fixedly connected with the air inlet assembly 10, the top ends of the two sides of the annular hopper 805 are fixedly connected with T-shaped isolation plates 802 on the inner side of the stand column 801, the outer side of the T-shaped isolation plate 802 is fixedly connected with the spraying assembly 11, the stand column 801 and the T-shaped isolation plate 802 of the isolation assembly 8 are not only structural members for separating the cold cabin 803 and the hot cabin 804, but also become the base for installing the spraying annular sleeve 1101 and the wall surface for guiding the airflow, the two sides of the stand column 801 are separated into the cold cabin 803 and the hot cabin 804 by the T-shaped isolation plate 802, the outer wall of the T-shaped isolation plate 802 is rotationally attached to the inner wall of the rotating cabinet 6, the cold cabin 803 and the hot cabin 804 are respectively communicated with the inside of the rotating cabinet 6, forming a stable and closed air conveying and supplying basis, ensuring the structural rigidity and sealing performance of the environmental control system.

[0043] As shown in Figures 4 to 6 The rotating cabinet 6 is internally provided with a plurality of compartments 601, which constitute independent and standardized culture units, realize batch and modular management of strain culture, a plurality of compartments 601 on the same layer are rotationally corresponding with a plurality of irradiation lamps 904, the two sides of the compartment 601 are respectively provided with a baffle 602, the top end of the baffle 602 is slidingly connected with the culture medium 7, for fixing the position of the culture medium 7, and also facilitating the access operation of the culture medium 7, the rotating cabinet 6 is fixedly connected with a single spool 603 at the top end, the single spool 603 is respectively frictionally connected with two belts 906, and synchronously moves with the irradiation frame 9, ensuring sufficient illumination effect.

[0044] As shown in Figures 6 to 8As shown, the spraying assembly 11 comprises a plurality of annular sleeves 1101, the outer side of the annular sleeve 1101 is rotatably connected with a sealing annular plate 1102, the surface of the sealing annular plate 1102 is mounted with an extension pipe 1103, the end of the extension pipe 1103 away from the sealing annular plate 1102 is provided with a spray port 1104, and the plurality of annular sleeves 1101 are respectively arranged on the upper and lower ends of the inner side of each compartment 601. Even if the rotating cabinet 6 drives the culture medium 7 to rotate continuously, the spatial orientation of the spray port 1104 can still be basically stable through the relative rotation of the sealing annular plate 1102, so as to ensure that the spraying direction is always aligned with the culture area. The two spray ports 1104 located inside the same compartment 601 are oppositely arranged, different liquids are sprayed from the upper and lower directions at the same time, and omnidirectional uniform humidification and nourishment are performed. The inner sides of the plurality of annular sleeves 1101 located at the top end of the inner side of the compartment 601 are fixedly connected with an upper connecting pipe 1106, and the inner sides of the plurality of annular sleeves 1101 located at the bottom end of the inner side of the compartment 601 are fixedly connected with a lower connecting pipe 1105. The lower connecting pipe 1105 and the upper connecting pipe 1106 are respectively fixedly connected with the anti-settling liquid storage assembly 2, and the upper and lower connecting pipes 1105 are respectively connected to different liquid sources of the anti-settling liquid storage assembly 2. In the simulation of the growth promotion period, nutrient solution can be sprayed from the upper and lower directions at the same time; in the simulation of the stress period, salt water can be sprayed from the lower direction alone to simulate the increase of rhizosphere salt, so as to realize the simulation of the direction and intensity of salt stress.

[0045] As shown in Figure 9 , the air inlet assembly 10 comprises a first pipeline 1001 and a second pipeline 1002. The second pipeline 1002 is fixedly communicated with the cold cabin 803. A circulating air duct 1003 is fixedly connected to the middle part of the second pipeline 1002. A semiconductor heat sink 1008 is fixedly connected inside the circulating air duct 1003. One end of the semiconductor heat sink 1008 is fixedly connected with the second pipeline 1002. The circulating air duct 1003 is fixedly connected with the supporting bottom bin 1. A first electric fan 1005 is installed at the penetrating end of the circulating air duct 1003. The first pipeline 1001 is fixedly communicated with the hot cabin 804. The middle part of the second pipeline 1002 and the middle part of the first pipeline 1001 are fixedly connected with an air inlet duct 1004. A second electric fan 1007 is fixedly connected inside the air inlet duct 1004. The air inlet duct 1004 is fixedly connected with the supporting bottom bin 1. A filter screen 1006 is installed at the penetrating end of the air inlet duct 1004. Two sets of independent air ducts and fans are formed, which ensures that the generation and conveying paths of cold air and hot air are completely independent, fundamentally avoids the advance mixing of air flow and temperature interference, and guarantees that the air temperature sent to the cold cabin 803 and the hot cabin 804 has the highest purity and stability.

[0046] As shown in Figure 10 , the culture medium 7 is provided with a high-density gauze 701 at the bottom end, which is convenient for salt stress simulation. The culture medium 7 is located between the spraying assemblies 11, and the spraying assemblies 11 are fixedly connected with the anti-settling liquid storage assembly 2.

[0047] As Figures 11 to 12 shown, the anti-settling liquid storage assembly 2 includes a pull plate 201, the inner side of the pull plate 201 is fixedly connected with a stand 204, the bottom ends of the two sides of the stand 204 are slidably connected with a fixed slide 205, the fixed slide 205 is fixedly connected with the support bottom bin 1, the top end of the side close to the pull plate 201 of the stand 204 is rotatably connected with a fixed frame 203, the inner side of the fixed frame 203 is slidably connected with a square bucket 202, a plurality of square buckets 202 are independently placed in the fixed frame 203 in a sliding manner, and the square buckets 202 are respectively filled with clean water, salt water and nutrient solution. A plurality of square buckets 202 are independently placed in the fixed frame 203 in a sliding manner, and are respectively filled with clean water, salt water, nutrient solution and other different media. This design facilitates users to individually take out any bucket for cleaning, replenishment or replacement of liquid, realizes modular management of the storage unit, is flexible to operate, avoids cross contamination, and the bottom end of the side away from the pull plate 201 of the fixed frame 203 is fixedly connected with an extension plate 212, one end of the extension plate 212 is slidably connected with a roller 214, the roller 214 is slidably connected with the fixed slide 205, the penetrating end of the roller 214 is rotatably connected with a push handle 213, the bottom end of one side of the fixed slide 205 is fixedly connected with a plurality of mounting sleeves 209, the penetrating end of the plurality of mounting sleeves 209 is rotatably connected with a transmission rod 208, the middle part of the transmission rod 208 is fixedly connected with a plurality of cams 210, the surface of the cam 210 is slidably attached to the surface of the push handle 213, one end of the transmission rod 208 is rotatably connected with a first motor 211, the power output end of the first motor 211 is fixedly connected with the transmission rod 208, the first motor 211 is fixedly connected with the fixed slide 205, and the square bucket 202 in the fixed slide 205 is driven to swing regularly. This low-amplitude and high-frequency shaking can effectively prevent the solute from settling, ensure that the concentration of the solution in the bucket remains uniform at all times, and provide a physical guarantee for subsequent precise quantitative spraying, the top end of the side close to the fixed slide 205 of the stand 204 is fixedly connected with a pump 206, the water inlet of the pump 206 is located in the inner bottom end of the square bucket 202, the water outlet of the pump 206 connected with the square bucket 202 filled with clean water and nutrient solution is fixedly connected with a mixing tank 207, the mixing tank 207 is fixedly connected with the upper connecting pipe 1106, and the water outlet of the pump 206 connected with the square bucket 202 filled with salt water is fixedly connected with the lower connecting pipe 1105. The water inlet of the pump 206 penetrates the bottom of each bucket to directly extract homogeneous solution. The conveying path is intelligently divided: the pump 206 connected with the mixing tank 207 is responsible for pumping clean water and nutrient solution into the tank as needed and mixing them in real time to form culture solution, which is supplied to the upper layer of the spraying assembly 11 through the upper connecting pipe 1106; and the salt water is directly pumped out by the independent pump 206 and supplied to the lower layer through the lower connecting pipe 1105. This design realizes independent or mixed supply of liquids with different properties, and lays a foundation for simulating complex scenarios such as spraying salt water from the root or spraying nutrient solution from the upper layer under salt stress.

[0048] The working principle of the embodiment is as follows:

[0049] The strains and substrates are placed in the culture medium 7, and then each culture medium 7 is placed in each compartment 601, at this time, the sealing door 503 and the arc-shaped door 502 closing device are closed, and then the device is started, the second motor 902 drives the irradiation frame 9 and the rotating cabinet 6 to rotate slowly through the belt 906, and the culture medium 7 periodically passes through the cold cabin 803 and the hot cabin 804 separated by the isolation assembly 8 with the rotating cabinet 6.

[0050] At the same time, the air inlet assembly 10 starts to work: the second electric fan 1007 sends the filtered fresh air outside into the first pipeline 1001 and the second pipeline 1002 through the air inlet duct 1004. The air flowing through the circulating air duct 1003 is cooled by the semiconductor heat sink 1008 to form cold air into the cold cabin 803; the air flowing through the second pipeline 1002 is sent into the hot cabin 804 and is naturally heated by the irradiation lamp 904, so that the rotating culture medium 7 alternately stays in a low-temperature and a high-temperature environment, simulating the day and night temperature cycle in nature.

[0051] The irradiation lamp 904 on the irradiation frame 9 rotates with the rotating rod 901, and the light thereof sweeps through the passing culture medium 7 at a changing angle, simulating the daily cycle change of sunlight.

[0052] The first motor 211 in the anti-settling liquid storage assembly 2 drives the cam mechanism to make each liquid storage barrel continuously swing slightly, preventing the solution from settling. According to the preset program, the control system instructs the corresponding pump 206 to work. For example, in the simulation of the plant growth stage, the mixed tank 207 can be controlled to adjust the mixing ratio of water and nutrient solution, and the mixed culture solution is pumped to the upper connecting pipe 1106 of the spraying assembly 11, and the culture medium 7 is humidified and fed by the upper spray port 1104; in the simulation of the stress or night stage, the salt water pump can be controlled to directly pump the salt solution to the lower connecting pipe 1105, mainly to spray the culture medium 7 from the bottom spray port 1104 to simulate the actual environment of the saline-alkali soil root.

[0053] The user can set the temperature, light cycle, spraying program and other parameters through the monitoring panel 4, and monitor the growth state of the strains in real time through the observation window 505 on the multi-layer cabin assembly 5, and can open the sealing door 503 to access the culture medium 7 when necessary.

[0054] The device realizes dynamic and complex simulation of the temperature, light, humidity, salt and nutrition of the natural environment of the saline-alkali soil through the precise cooperation of the above-mentioned subsystems, and provides a platform for efficient cultivation of natural ecological strains with adaptability.

[0055] The above only describes the preferred embodiment of the present application, and is not used to limit the protection scope of the present application.

Claims

1. A device for cultivating natural ecological microbial strains for saline-alkali land improvement, comprising a supporting chamber (1), characterized in that: A plurality of anti-precipitation liquid storage components (2) are slidably connected through one side of the support base (1). A sealed chamber (3) is fixedly connected to the top of the support base (1). Multi-layer chamber components (5) are fixedly connected to both sides of the sealed chamber (3). A monitoring panel (4) is provided on the surface of the sealed chamber (3). An irradiation frame (9) is rotatably connected to the inside of the sealed chamber (3). An isolation component (8) is fixedly connected to the middle of the inside of the multi-layer chamber component (5). A rotating cabinet (6) is rotatably connected to the outside of the isolation component (8). A plurality of culture media (7) are slidably connected inside the rotating cabinet (6). The rotating cabinet (6) and the irradiation frame (9) are rotatably connected to each other in an alternating manner. A spraying assembly (11) is fixedly connected to the inner side of the two isolation components (8), and an air intake assembly (10) is fixedly connected between the bottom ends of the two isolation components (8). The culture medium (7) is provided with a high-density mesh (701) at the bottom. The culture medium (7) is located between the spraying components (11). The spraying components (11) are fixedly connected to the anti-precipitation storage components (2). The isolation assembly (8) includes a column (801), the bottom end of which is fixedly connected to the support chamber (1), a ring bucket (805) is fixedly connected to the middle of the column (801), the two sides of the ring bucket (805) are fixedly connected to the air intake assembly (10), the two sides of the top of the ring bucket (805) are fixedly connected to the inner side of the column (801), and the outer side of the T-shaped isolation plate (802) is fixedly connected to the spraying assembly (11). The two sides of the column (801) are divided into a cold compartment (803) and a hot compartment (804) by the T-shaped isolation plate (802). The outer wall of the T-shaped isolation plate (802) rotates and fits against the inner wall of the rotating cabinet (6). The cold compartment (803) and the hot compartment (804) are respectively connected to the interior of the rotating cabinet (6). The irradiation frame (9) includes a rotating rod (901), a second motor (902) is rotatably connected to the bottom end of the rotating rod (901), the power output end of the second motor (902) is fixedly connected to the support base (1), a number of six-claw turntables (903) are arranged and fixedly connected in an array in the middle of the rotating rod (901), a number of irradiation lamps (904) are provided at the bottom end of the six-claw turntables (903), and a double-groove roller (905) is fixedly connected to the top end of the rotating rod (901), and two belts (906) are respectively frictionally driven connected to the surface of the double-groove roller (905).

2. The device for cultivating natural ecological microbial strains for saline-alkali land improvement according to claim 1, characterized in that, The rotating cabinet (6) has several compartments (601) inside. Several compartments (601) on the same layer are rotated and correspond to several illumination lamps (904). A baffle (602) is installed on both sides of the compartment (601). The top of the baffle (602) is slidably connected to the culture medium (7). A single roller (603) is fixedly connected to the top of the rotating cabinet (6). The single roller (603) is frictionally connected to two belts (906).

3. The device for cultivating natural ecological microbial strains for saline-alkali land improvement according to claim 1, characterized in that, The spraying assembly (11) includes several annular sleeves (1101). A sealing annular plate (1102) is rotatably connected to the outer side of each annular sleeve (1101). An extension tube (1103) is installed on the surface of the sealing annular plate (1102). A nozzle (1104) is provided at the end of the extension tube (1103) away from the sealing annular plate (1102). Several annular sleeves (1101) are respectively arranged at the upper and lower ends of the inner side of each compartment (601) and are located in the same compartment. (601) The two nozzles (1104) inside are arranged opposite to each other. The inner sides of several annular sleeves (1101) located at the top of the inner side of the compartment (601) are fixedly connected to an upper connecting pipe (1106). The inner sides of several annular sleeves (1101) located at the bottom of the inner side of the compartment (601) are fixedly connected to a lower connecting pipe (1105). The lower connecting pipe (1105) and the upper connecting pipe (1106) are respectively fixedly connected to the anti-sedimentation storage component (2).

4. The device for cultivating natural ecological microbial strains for saline-alkali land improvement according to claim 1, characterized in that, The air intake assembly (10) includes a first pipe (1001) and a second pipe (1002). The second pipe (1002) is fixedly connected to the cold compartment (803). A circulating air duct (1003) is fixedly connected to the middle of the second pipe (1002). A semiconductor heat sink (1008) is fixedly connected inside the circulating air duct (1003). One end of the semiconductor heat sink (1008) is fixedly connected to the second pipe (1002). The circulating air duct (1003) is fixedly connected to the supporting base (1). A first electric fan (1005) is installed at the through end of the air duct (1003). The first pipe (1001) is fixedly connected to the hot chamber (804). The middle part of the second pipe (1002) and the middle part of the first pipe (1001) are fixedly connected to the air intake duct (1004). A second electric fan (1007) is fixedly connected inside the air intake duct (1004). The air intake duct (1004) is fixedly connected to the support base (1). A filter screen (1006) is installed at the through end of the air intake duct (1004).

5. The device for cultivating natural ecological microbial strains for saline-alkali land improvement according to claim 1, characterized in that, The anti-sedimentation storage component (2) includes a pull plate (201), a support frame (204) is fixedly connected to the inner side of the pull plate (201), and fixed slides (205) are slidably connected to the bottom ends of both sides of the support frame (204). The fixed slides (205) are fixedly connected to the supporting base (1). A fixed frame (203) is rotatably connected to the top of the support frame (204) near the pull plate (201). A square water bucket (202) is slidably connected to the inner side of the fixed frame (203). Several square water buckets (202) are included. The interior is filled with clean water, salt water, and nutrient solution. An extension plate (212) is fixedly connected to the bottom end of the fixed frame (203) away from the pull plate (201). A roller (214) is slidably connected to one end of the extension plate (212). The roller (214) is slidably connected to the fixed slide (205). A push handle (213) is rotatably connected to the end of the roller (214). Several mounting sleeves (209) are fixedly connected to one side of the bottom of the fixed slide (205). Several mounting sleeves (209)... A transmission rod (208) is rotatably connected between 9) and 9. Several cams (210) are fixedly connected to the middle of the transmission rod (208). The surface of the cams (210) slides against the surface of the push handle (213). A first motor (211) is rotatably connected to one end of the transmission rod (208). The power output end of the first motor (211) is fixedly connected to the transmission rod (208). The first motor (211) is fixedly connected to the fixed slide (205). The upright frame (204) is close to the fixed slide (205). 205) A pump (206) is fixedly connected to the top of one side. The inlet of the pump (206) is located at the bottom of the inside of the square water bucket (202). A mixing tank (207) is fixedly connected to the outlet of the pump (206) which is connected to the square water bucket (202) containing clean water and nutrient solution. The mixing tank (207) is fixedly connected to the upper connecting pipe (1106). The outlet of the pump (206) which is connected to the square water bucket (202) containing salt water is fixedly connected to the lower connecting pipe (1105).

6. The device for cultivating natural ecological microbial strains for saline-alkali land improvement according to claim 1, characterized in that, The multi-layer cabin assembly (5) includes a semi-circular sleeve (501), which is fixedly connected to the sealed cabin (3). The inner wall of the sealed cabin (3) is rotated and fitted with the rotating cabinet (6). A sealing door (503) is slidably connected to the middle of the semi-circular sleeve (501) from top to bottom. An observation window (505) is provided in the middle of the sealing door (503). A light shield (504) is slidably connected to one side of the surface of the observation window (505). An arc-shaped door (502) is slidably connected to the outer surface of the semi-circular sleeve (501).

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