Abalone peptide capable of enhancing immunity, resisting wrinkles and whitening skin and preparation method of abalone peptide
By combining enzymatic hydrolysis-glycyrrhizic acid synergistic grafting modification of abalone peptides with 2-hydroxy-4-methoxyacetophenone, the problems of unstable structure and low transdermal absorption of abalone peptides were solved, achieving high stability and significant immunomodulatory, anti-wrinkle and whitening effects.
Patent Information
- Application Number
- CN202511639069.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-11-10
- Publication Date
- 2026-01-23
- Estimated Expiration
- Not applicable · inactive patent
AI Technical Summary
Existing abalone polypeptide structures are unstable, easily degraded and inactivated, and have low transdermal absorption rates. Furthermore, existing whitening and anti-wrinkle active substances are highly irritating and have poor stability, making it difficult to simultaneously achieve skin immune regulation and anti-wrinkle protection functions.
Abalone peptides modified by enzymatic hydrolysis and glycyrrhizic acid synergistic grafting were combined with 2-hydroxy-4-methoxyacetophenone. The structural stability and lipophilicity of the peptides were improved by amidation reaction, forming a synergistic immune-enhancing and antioxidant activity system.
It significantly improves the stability and skin penetration of abalone peptides, enhances immune regulation and anti-wrinkle and whitening effects, and achieves safe and efficient skin conditioning.
Smart Images
Figure CN121370652A_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The present application relates to the technical field of biochemical and functional cosmetic materials, in particular to an abalone peptide for enhancing immunity and anti-wrinkle whitening and a preparation method thereof. BACKGROUND
[0002] In recent years, with the emphasis on skin health and immune regulation, bioactive peptide raw materials with both cosmetic and physiological regulation functions have gradually become a research hotspot. Among them, abalone polypeptides are rich in lysine, proline, arginine and various trace elements, and have good immune enhancement, antioxidant and anti-inflammatory properties. However, there are still the following problems in its application: 1. The natural abalone polypeptide has strong polarity, poor structural stability, is easy to degrade and inactivate, and is difficult to maintain activity in the formula system; 2. Its molecular weight distribution is wide, and the skin penetration ability is limited, resulting in low absorption rate and insignificant functional effect when used externally; 3. The existing modification methods of polypeptides are mainly acid hydrolysis, alkaline hydrolysis or single enzymatic hydrolysis, and there is a lack of mild chemical grafting modification means to improve its bioavailability. The commonly used whitening and anti-wrinkle active substances on the market are mainly small molecule compounds with single action, such as arbutin, kojic acid, hydroquinone, etc. Although these substances have the effect of inhibiting tyrosinase activity, they have strong irritability, poor stability, and insufficient synergy, and it is difficult to balance the functions of skin immune regulation and anti-wrinkle protection.
[0003] The existing technology urgently needs a new biopeptide composite system with immune enhancement, whitening and anti-wrinkle effects. This system should not only maintain the physiological activity of polypeptides, but also improve its stability and permeability through structural modification, and synergize with organic small molecules with anti-tyrosinase activity to achieve safe, efficient and sustainable skin conditioning effects. SUMMARY
[0004] In order to overcome the problems of unstable structure, easy degradation and inactivation, low transdermal absorption rate and single function of abalone polypeptides, the present application aims to provide an abalone peptide for enhancing immunity and anti-wrinkle whitening and a preparation method thereof. The present application uses an enzyme-hydrolyzed-glycyrrhizic acid synergistically grafted abalone polypeptide as the main active ingredient, improves its structural stability and lipophilicity through the amidation reaction between glycyrrhizic acid monosodium salt and abalone polypeptide molecules, and is compounded with 2-hydroxy-4-methoxyphenylacetone with anti-tyrosinase activity to form an active system with synergistic immune enhancement and antioxidant activity. The abalone peptide obtained by the present application has high stability, good skin absorption and significant dual functions of immune regulation and anti-wrinkle whitening.
[0005] The purpose of the present application can be achieved by the following technical solutions: The abalone peptide for enhancing immunity, anti-wrinkle and whitening comprises the following raw materials in parts by weight: 50-80 parts of abalone polypeptide modified by enzymolysis-glycyrrhizic acid grafting; 5-20 parts of 2-hydroxy-4-methoxyphenylacetophenone; 2-6 parts of anti-oxidation auxiliary agent; 3-10 parts of moisturizing agent; 0.5-2 parts of pH stabilizer; 0.1-0.3 parts of preservative auxiliary agent; and 150-250 parts of deionized water; wherein the abalone polypeptide modified by enzymolysis-glycyrrhizic acid grafting is a modified product obtained by grafting reaction of amide under the action of glycyrrhizic acid monosodium salt after complex enzymolysis of abalone polypeptide, and has high stability and immunity activity; and the 2-hydroxy-4-methoxyphenylacetophenone is an aromatic small molecule with anti-tyrosinase activity and free radical scavenging capacity, which is helpful to realize skin whitening and antioxidant protection.
[0006] Optionally, the abalone polypeptide modified by enzymolysis-glycyrrhizic acid grafting comprises the following raw materials in parts by weight: 60-100 parts of abalone meat powder; 10-20 parts of glycyrrhizic acid monosodium salt; 1-3 parts of trypsin; 0.5-2 parts of alkaline protease; 1-3 parts of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride; 0.5-1.5 parts of N-hydroxysuccinimide; and 200-300 parts of deionized water.
[0007] Optionally, the preparation method of the abalone polypeptide modified by enzymolysis-glycyrrhizic acid grafting comprises the following steps: (1) abalone meat powder is added into deionized water for stirring and dissolving, pH is adjusted, trypsin and alkaline protease are added for enzymolysis, and after the enzymolysis is completed, filtration and freeze-drying are performed to obtain abalone polypeptide powder; (2) the abalone polypeptide powder is dissolved in deionized water, glycyrrhizic acid monosodium salt, 1-ethyl-3-dimethylaminopropyl carbodiimide hydrochloride and N-hydroxysuccinimide are added, stirring is performed, dialysis purification is performed after the reaction is completed, and freeze-drying is performed to obtain the abalone polypeptide modified by enzymolysis-glycyrrhizic acid grafting.
[0008] Optionally, the enzymolysis condition of step (1) is pH 7.0-8.0, temperature 35-45°C, and reaction time 2-4 hours.
[0009] Optionally, the grafting modification condition of step (2) is pH 7.0-7.5, temperature 35-45°C, and stirring reaction time 4-8 hours.
[0010] Optionally, the anti-oxidation auxiliary agent is a mixture of ascorbic acid and glutathione in a mass ratio of 1:1; the moisturizing agent is a mixture of glycerol and sodium hyaluronate in a mass ratio of 2:1; the pH stabilizer is a mixture of sodium citrate and triethanolamine in a mass ratio of 1:1; and the preservative auxiliary agent is a mixture of phenoxyethanol and caprylhydroxamic acid in a mass ratio of 3:1.
[0011] Optionally, a preparation method of abalone peptide for enhancing immunity and anti-wrinkle whitening, comprising the following steps: S1, the enzyme hydrolysis-glycyrrhizic acid synergistic grafted modified abalone polypeptide, 2-hydroxy-4-methoxy acetophenone, antioxidant, humectant, pH stabilizer and preservative aid are added into deionized water, and stirred and mixed to form a uniform solution; S2, the uniform solution is subjected to filtration and sterilization treatment to obtain a filtrate; S3, the filtrate is subjected to aseptic filling and sealing under sterile conditions to obtain the abalone peptide product for enhancing immunity and anti-wrinkle whitening.
[0012] Optionally, the mixing conditions of step S1 are that the temperature is 25-35 DEG C, the stirring speed is 300-500 r / min, and the stirring time is 20-40 minutes.
[0013] Optionally, the filtration conditions of step S2 are that the filtration is performed by using a filter membrane with a pore size of 0.22-0.45 microns.
[0014] Optionally, the filling conditions of step S3 are that the sealing filling is performed in a sterile environment with a cleanliness of 100,000 levels.
[0015] The beneficial effects of the present application are: The present application introduces glycyrrhizic acid monosodium salt under mild conditions to perform amide grafting modification on abalone polypeptide, so that a triterpenoid domain containing carboxyl and hydroxyl groups is formed on the surface of the polypeptide molecules, thereby constructing a stable structure with hydrophilic-hydrophobic biphasic distribution, significantly improving the lipid solubility and skin penetration ability of the abalone polypeptide, and enhancing its retention and bioavailability in the stratum corneum; the modified polypeptide retains a higher proportion of active amino sites, can specifically bind to skin cell membrane receptors, and improve cell immune regulation function; after compounding with 2-hydroxy-4-methoxy acetophenone, the system forms a stable resonance antioxidant structure, exhibits a synergistic free radical scavenging and tyrosinase inhibition capacity, and realizes multiple active effects of immune enhancement and anti-wrinkle whitening. BRIEF DESCRIPTION OF DRAWINGS
[0016] The present application will be further described below in combination with the drawings.
[0017] Figure 1 It is an infrared spectrum comparison chart of abalone polypeptide and enzyme hydrolysis-glycyrrhizic acid synergistically grafted modified abalone polypeptide; Figure 2 It is a comparison chart of free radical scavenging rate, tyrosinase inhibition rate and collagen synthesis promotion rate of different proportion samples. DETAILED DESCRIPTION
[0018] The application will be further described in conjunction with specific examples, but the application is not limited to the following examples, and equivalent adjustments made without departing from the spirit and essence of the application should also be considered to fall within the scope of the application.
[0019] Example 1 The purpose of this example is to verify the strongest performance of the system stability, antioxidant performance and anti-wrinkle whitening effect when the modified abalone peptide and active small molecule content are at the upper limit ratio.
[0020] S1, take 100 parts of abalone meat powder, add 250 parts of deionized water and stir to dissolve, adjust the pH to 7.8, add 3 parts of trypsin and 2 parts of alkaline protease, and enzymolysis at 40℃ for 3 hours. After the enzymolysis is completed, filter and freeze-dry to obtain abalone polypeptide powder; dissolve 100 parts of abalone polypeptide powder in 300 parts of deionized water, add 20 parts of monosodium salt of glycyrrhizic acid, 3 parts of 1-ethyl-3-dimethylaminopropyl carbodiimide hydrochloride, and 1.5 parts of N-hydroxysuccinimide, maintain pH 7.3, and stir at 40℃ for 6 hours. After the reaction is completed, dialysis purification and freeze-drying are carried out to obtain the abalone polypeptide modified by enzymolysis-glycyrrhizic acid grafting. S2, take 80 parts of modified abalone polypeptide, 20 parts of 2-hydroxy-4-methoxy acetophenone, 6 parts of ascorbic acid and glutathione complex antioxidant, 10 parts of glycerol and sodium hyaluronate complex moisturizing agent, 2 parts of sodium citrate and triethanolamine complex pH stabilizer, and 0.3 parts of preservative aid, add 250 parts of deionized water, and stir at 400 rpm for 30 minutes at 30℃ to form a uniform solution; S3, the obtained solution is filtered through a 0.22 micron filter membrane to remove bacteria, and sterilely filled in a cleanliness of 100,000 level environment to obtain abalone peptide products for enhancing immunity and anti-wrinkle whitening.
[0021] Example 2 The purpose of this example is to evaluate the stability and functional balance of the product under the intermediate ratio condition, and to obtain the best process parameters suitable for industrialization.
[0022] S1, take 80 parts of abalone meat powder, add 220 parts of deionized water and stir to dissolve, adjust the pH to 7.5, add 2 parts of trypsin and 1 part of alkaline protease, and enzymolysis at 40℃ for 3 hours. After the enzymolysis is completed, filter and freeze-dry to obtain abalone polypeptide powder; dissolve 80 parts of abalone polypeptide powder in 250 parts of deionized water, add 15 parts of monosodium salt of glycyrrhizic acid, 2 parts of 1-ethyl-3-dimethylaminopropyl carbodiimide hydrochloride, and 1 part of N-hydroxysuccinimide, control the pH at 7.2, and stir at 38℃ for 6 hours. After dialysis purification, freeze-drying is carried out to obtain the abalone polypeptide modified by enzymolysis-glycyrrhizic acid grafting. S2, take the modified abalone polypeptide 65 parts, 2-hydroxy-4-methoxy acetophenone 10 parts, antioxidant 4 parts, humectant 6 parts, pH stabilizer 1 part, preservative 0.2 parts, add deionized water 200 parts, stirring at 400 rpm for 30 minutes at 28℃, get transparent and uniform solution; S3, the solution is filtered through 0.30 micron filter membrane to remove bacteria, and is filled and sealed in a clean environment of 100,000 levels to obtain an abalone peptide product for enhancing immunity, anti-wrinkle and whitening.
[0023] Example 3 The purpose of this example is to investigate the stability of the system when the content of active ingredient is low and the minimum effective concentration range, and to determine the economic performance balance point of the formula.
[0024] S1, take abalone meat powder 60 parts, add deionized water 200 parts and stir to dissolve, adjust pH to 7.0, add trypsin 1 part and alkaline protease 0.5 part, and enzymolysis at 37℃ for 2 hours, filter and freeze-dry to obtain abalone polypeptide powder; dissolve the abalone polypeptide powder 60 parts in deionized water 200 parts, add glycyrrhizic acid monosodium salt 10 parts, 1-ethyl-3-dimethylaminopropyl carbodiimide hydrochloride 1 part, N-hydroxysuccinimide 0.5 part, keep pH at 7.0, stir at 35℃ for 4 hours, purify by dialysis and freeze-dry to obtain modified abalone polypeptide; S2, take the modified abalone polypeptide 65 parts, 2-hydroxy-4-methoxy acetophenone 10 parts, antioxidant 4 parts, humectant 6 parts, pH stabilizer 1 part, preservative 0.2 parts, add deionized water 200 parts, stirring at 400 rpm for 30 minutes at 28℃, get transparent and uniform solution; S3, the solution is filtered through 0.30 micron filter membrane to remove bacteria, and is filled and sealed in a clean environment of 100,000 levels to obtain an abalone peptide product for enhancing immunity, anti-wrinkle and whitening.
[0025] Comparative Example 1 The purpose of this comparative example is to verify the difference in skin anti-wrinkle and whitening performance of abalone polypeptide treated only by complex enzymolysis without glycyrrhizic acid graft modification.
[0026] S1, take abalone meat powder 60 parts, add deionized water 200 parts and stir to dissolve, adjust pH to 7.0, add trypsin 1 part and alkaline protease 0.5 part, and enzymolysis at 37℃ for 2 hours, filter and freeze-dry to obtain abalone polypeptide powder; dissolve the abalone polypeptide powder 60 parts in deionized water 200 parts, add glycyrrhizic acid monosodium salt 10 parts, 1-ethyl-3-dimethylaminopropyl carbodiimide hydrochloride 1 part, N-hydroxysuccinimide 0.5 part, keep pH at 7.0, stir at 35℃ for 4 hours, purify by dialysis and freeze-dry to obtain modified abalone polypeptide; S2, take abalone polypeptide 65 parts, 2-hydroxy-4-methoxy acetophenone 10 parts, antioxidant aid 4 parts, humectant 6 parts, pH stabilizer 1 part, preservative aid 0.2 parts, add deionized water 200 parts, stir at 400 rpm for 30 minutes at 28°C, until a uniform transparent solution is formed; S3, the obtained solution is filtered through a 0.30 μm filter to remove bacteria, and is filled and sealed in a 100,000-level clean environment to obtain an abalone peptide sample modified only by enzymolysis.
[0027] Comparative Example 2 The purpose of this comparative example is to verify the degree of structural modification and functional difference of abalone polypeptide when only glycyrrhizic acid graft modification is performed without complex enzymatic pretreatment.
[0028] S1, take abalone meat powder 80 parts, add deionized water 250 parts and stir to dissolve, do not add any enzyme preparation, after complete dissolution, add glycyrrhizic acid monosodium salt 15 parts, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride 2 parts and N-hydroxysuccinimide 1 part, adjust pH to 7.2, stir at 38°C for 6 hours, after the reaction is completed, the system is dialyzed to remove small molecule impurities, then freeze-dried to obtain abalone polypeptide modified only by glycyrrhizic acid grafting; S2, take modified abalone polypeptide 65 parts, 2-hydroxy-4-methoxy acetophenone 10 parts, antioxidant aid 4 parts, humectant 6 parts, pH stabilizer 1 part, preservative aid 0.2 parts, add deionized water 200 parts, stir at 400 rpm for 30 minutes at 28°C, to prepare a uniform transparent solution; S3, the solution is filtered through a 0.30 μm filter to remove bacteria, and is filled and sealed in a 100,000-level clean environment to obtain an abalone peptide sample modified only by glycyrrhizic acid.
[0029] Comparative Example 3 The purpose of this comparative example is to verify the influence on overall antioxidant and whitening performance when the system does not contain 2-hydroxy-4-methoxy acetophenone.
[0030] S1, take abalone meat powder 80 parts, add deionized water 220 parts and stir to dissolve, adjust pH to 7.5, add trypsin 2 parts and alkaline protease 1 part, and enzymolysis at 40°C for 3 hours, after the enzymolysis is completed, filter out impurities and freeze-dry to obtain abalone polypeptide powder; dissolve the abalone polypeptide powder 80 parts in deionized water 250 parts, add glycyrrhizic acid monosodium salt 15 parts, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride 2 parts, N-hydroxysuccinimide 1 part, control pH to 7.2, temperature to 38°C, stir for 6 hours, after the reaction is completed, dialyze and freeze-dry to obtain abalone polypeptide modified by enzymolysis-glycyrrhizic acid grafting; S2, 65 parts of the modified abalone polypeptide, 4 parts of the antioxidant, 6 parts of the humectant, 1 part of the pH stabilizer, 0.2 parts of the preservative aid, and 200 parts of deionized water were added, stirred at 400 rpm for 30 minutes at 28°C to form a transparent and uniform solution; S3, the obtained solution was filtered through a 0.30 μm filter membrane to remove bacteria, and was filled and sealed in a 10 million level clean environment to obtain an abalone peptide sample without organic small molecules.
[0031] Performance test 1. Antioxidant performance test The sample solutions of Examples 1-3 and Comparative Examples 1-3 were taken, and were adjusted to the same concentration and incubated at 37°C. The change in free radical scavenging rate was detected, and the free radical scavenging ability of different samples was evaluated by comparing the color change and light absorption intensity of the system after reaction. The results showed that Examples 1-3 containing the "enzyme hydrolysis-glycyrrhizic acid synergistically grafted modified abalone peptide" and "2-hydroxy-4-methoxyphenylacetone" had significantly higher free radical scavenging efficiency, while the free radical residual rate of the single modification group or the group without organic small molecules was significantly increased, indicating that the synergistic modification system could effectively enhance the antioxidant performance and improve the stable scavenging effect of free radicals.
[0032] 2. Whitening performance test A cell model simulating melanin generation was selected, and the samples of Examples and Comparative Examples were added to the system, and the tyrosinase activity and melanin generation amount in the system were measured after 48 hours of culture at 37°C and 5% carbon dioxide. The comparison results showed that the Example group containing 2-hydroxy-4-methoxyphenylacetone significantly inhibited melanin generation, and showed good whitening effect; while the melanin deposition of Comparative Example 3 sample without the component was obvious, indicating that the introduction of aromatic small molecules could significantly improve the whitening effect. In addition, the improvement of enzyme hydrolysis-glycyrrhizic acid synergistic structure on the skin-friendly property and absorption rate of polypeptide molecules further promoted the action of active ingredients.
[0033] 3. Anti-wrinkle and collagen promotion performance test A human fibroblast cell culture system was used, and the samples of Examples and Comparative Examples were added, and the collagen synthesis level and wrinkle-related enzyme activity change of the cells were observed under constant temperature culture conditions. The results showed that the samples of Examples 1-3 could significantly increase the collagen generation of the cells, and effectively reduce the elastase activity, indicating that the synergistically modified abalone peptide had excellent effect on promoting the stability of dermal layer structure and improving skin elasticity. The effects of Comparative Examples 1 and 2 which were only enzyme hydrolyzed or only glycyrrhizic acid modified were weaker, further proving the key contribution of the double modification mechanism to the anti-wrinkle performance.
[0034] 4. Stability and safety test The samples of examples and comparative examples were respectively sealed and stored at 40°C and 75% relative humidity for accelerated stability test, and the changes of appearance, color, odor and pH value were detected regularly. The results showed that the samples of examples remained transparent state after 30 days of storage, without obvious precipitation or odor change, and the pH fluctuation was less than 0.3, indicating that the formula system had good physicochemical stability. At the same time, through the preliminary screening of skin irritation, the samples of examples had no obvious irritation reaction to the simulated skin tissue, while there were certain unstable precipitates and slight irritation in comparative examples 2 and 3. Therefore, the complex system of enzymatic modification-glycyrrhizic acid synergistic modification and organic small molecule can significantly improve the long-term stability and use safety of the formula.
[0035] Table 1: Comparison of performance test results of each sample
[0036] According to the performance test results shown in Table 1 and Figure 2 Table 2, examples 1-3 all showed excellent comprehensive performance in various indicators, which were significantly better than the samples of three comparative examples. Among them, example 2 reached the highest value in the three core indicators of free radical scavenging rate, tyrosinase inhibition rate and collagen synthesis promotion rate, which were 93.8%, 88.9% and 84.5% respectively, showing significant synergistic effect of the system in the fields of antioxidant, whitening and anti-wrinkle. The performance of examples 1 and 3 was slightly lower than that of example 2, but was still significantly higher than that of the comparative examples, indicating that "enzymatic modification-glycyrrhizic acid synergistic grafting" and "aromatic small molecule complexation" were the key factors to enhance the function of active peptides.
[0037] Comparative example 1 only used complex enzymatic treatment, lacking glycyrrhizic acid grafting structure, and its free radical scavenging rate was only 61.5%, and the collagen synthesis promotion rate was less than 50%, indicating that simple enzymatic modification had limited effect on the activity of polypeptides; comparative example 2 only underwent glycyrrhizic acid grafting modification without enzymatic pretreatment, resulting in tight molecular structure and insufficient exposure of reaction sites, which showed low whitening and anti-wrinkle effect; comparative example 3 removed 2-hydroxy-4-methoxyphenylacetone, and the tyrosinase inhibition rate decreased to 65.9%, indicating that the aromatic small molecule played a key role in enhancing the antioxidant and complexation reactions in the system.
[0038] Through comprehensive analysis, it can be seen that the abalone polypeptide treated by enzymatic treatment forms a stable amide structure under the synergistic action of glycyrrhizic acid monosodium salt, which significantly improves the electron donor ability and free radical capture rate of the peptide chain; combined with the aromatic conjugated system and phenolic hydroxyl substitution effect of 2-hydroxy-4-methoxyphenylacetone, the overall system has higher antioxidant capacity and skin repair activity. This structural synergistic design realizes the significant improvement of bioactive peptides in antioxidant, whitening and anti-wrinkle performance, and provides an innovative solution for the application of functional cosmetic raw materials.
Claims
1. An abalone peptide for enhancing immunity and anti-wrinkle whitening, characterized in that, The abalone peptide comprises the following raw materials by weight: 50-80 parts of enzyme-glycyrrhizic acid synergistically grafted modified abalone polypeptide; 5-20 parts of 2-hydroxy-4-methoxyphenylacetone; 2-6 parts of antioxidant aid; 3-10 parts of humectant; 0.5-2 parts of pH stabilizer; 0.1-0.3 parts of preservative aid; and 150-250 parts of deionized water; wherein the enzyme-glycyrrhizic acid synergistically grafted modified abalone polypeptide refers to a modified product obtained by amide grafting reaction of abalone polypeptide after complex enzymolysis under the action of glycyrrhizic acid monosodium salt, having high stability and immunocompetence; and the 2-hydroxy-4-methoxyphenylacetone refers to an aromatic small molecule having tyrosinase activity and free radical scavenging ability, which is helpful to achieve skin whitening and antioxidant protection.
2. The abalone peptide for enhancing immunity and anti-wrinkle whitening according to claim 1, characterized in that, The enzyme-glycyrrhizic acid synergistically grafted modified abalone polypeptide comprises the following raw materials by weight: 60-100 parts of abalone meat powder; 10-20 parts of glycyrrhizic acid monosodium salt; 1-3 parts of trypsin; 0.5-2 parts of alkaline protease; 1-3 parts of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride; 0.5-1.5 parts of N-hydroxysuccinimide; and 200-300 parts of deionized water.
3. The abalone peptide for enhancing immunity and anti-wrinkle whitening according to claim 1 or 2, characterized in that, The preparation method of the enzyme-glycyrrhizic acid synergistically grafted modified abalone polypeptide comprises the following steps: (1) abalone meat powder is added into deionized water and stirred to dissolve, the pH is adjusted, trypsin and alkaline protease are added for enzymolysis, after the enzymolysis is completed, filtration and freeze-drying are performed to obtain abalone polypeptide powder; (2) the abalone polypeptide powder is dissolved in deionized water, glycyrrhizic acid monosodium salt, 1-ethyl-3-dimethylaminopropyl carbodiimide hydrochloride and N-hydroxysuccinimide are added, stirring is performed, after the reaction is completed, dialysis purification is performed and freeze-drying is performed to obtain the enzyme-glycyrrhizic acid synergistically grafted modified abalone polypeptide.
4. The abalone peptide for enhancing immunity and anti-wrinkle whitening according to claim 3, characterized in that, The enzymolysis conditions of step (1) are pH 7.0-8.0, temperature 35-45℃, and reaction time 2-4 hours.
5. The abalone peptide for enhancing immunity and anti-wrinkle whitening according to claim 3, characterized in that, The grafting modification conditions of step (2) are pH 7.0-7.5, temperature 35-45℃, and stirring reaction time 4-8 hours.
6. The abalone peptide for enhancing immunity and anti-wrinkle whitening according to claim 1, characterized in that, The antioxidant aid is a mixture of ascorbic acid and glutathione in a mass ratio of 1:1; the humectant is a mixture of glycerol and sodium hyaluronate in a mass ratio of 2:1; the pH stabilizer is a mixture of sodium citrate and triethanolamine in a mass ratio of 1:1; and the preservative aid is a mixture of phenoxyethanol and caprylhydroxamic acid in a mass ratio of 3:
1.
7. A method for preparing the abalone peptide for enhancing immunity and anti-wrinkle whitening, the abalone peptide for enhancing immunity and anti-wrinkle whitening being as claimed in any one of claims 1 to 6, characterized in that, The method comprises the following steps: S1, the enzyme-glycyrrhizic acid synergistically grafted modified abalone polypeptide, 2-hydroxy-4-methoxyphenylacetone, antioxidant aid, humectant, pH stabilizer and preservative aid are added into deionized water, stirring is performed to form a uniform solution; S2, the uniform solution is subjected to filtration and sterilization treatment to obtain a filtrate; S3, the filtrate is subjected to aseptic filling and sealing to obtain the abalone peptide product for enhancing immunity, anti-wrinkle and whitening.
8. The abalone peptide for enhancing immunity and anti-wrinkle whitening according to claim 7, characterized in that, The mixing conditions of step S1 are temperature 25-35℃, stirring speed 300-500 rpm, and stirring time 20-40 minutes.
9. The abalone peptide for enhancing immunity and anti-wrinkle whitening according to claim 7, characterized in that, The filtering condition of the step S2 is to filter and sterilize by using a filter membrane with a pore size of 0.22-0.45 microns.
10. The abalone peptide for enhancing immunity and anti-wrinkle whitening according to claim 7, characterized in that, The filling condition of the step S3 is to seal and fill in a sterile environment with a cleanliness of 100,000 levels.