Method for extracting bamboo leaf flavonoid and preparing bamboo leaf flavonoid health-care beverage and beverage

Abstract

The invention discloses a method for extracting bamboo leaf flavonoid and preparing a bamboo leaf flavonoid health-care beverage and beverage, and particularly relates to a method for preparing the bamboo leaf flavonoid health-care beverage and beverage by using nine high-benefit bamboo varieties in southern China as raw materials. The high-temperature-resistant coating is prepared from the following raw materials in parts by weight. The components comprise 70 parts of flavonoid compounds and other effective active substances extracted from dry bamboo leaf fragments by adopting a complex enzyme-microwave method, 16 parts of polysaccharide extracted from hericium erinaceus by adopting a conventional hot water extraction method, 17 parts of effective active substances extracted from plumbago indica by adopting hot water, 9 parts of eurycoma longifolia, 26 parts of effective active substances of beautiful millettia roots and 15 parts of effective active substances of polygonatum kingianum. The bamboo leaf flavonoid health-care beverage and the bamboo leaf beverage integrate the nutrients and other effective components of the bamboo leaf flavonoid and the hericium erinaceus and plumbago indica extract liquor, become an excellent bamboo leaf flavonoid health-care beverage and the bamboo leaf beverage, and have the effects of eliminating various types of reactive oxygen free radicals and inhibiting lipid peroxidation after being drunk usually; the vitamin C can protect brain blood vessels, block nitrosation reaction and eliminate nitrous ammonia, and can resist fatigue and aging as vitamin C.

Description

TECHNICAL FIELD

[0001] The present invention relates to the field of product health drinks and beverages. The present invention provides a bamboo leaf flavonoid extract health drink and beverage, and a method for preparing the bamboo leaf flavonoid health drink and beverage using bamboo leaves of nine high-yield bamboo species in southern China as raw materials. BACKGROUND

[0002] The bamboo leaves of nine high-yield bamboo species (Phyllostachys edulis, Dendrocalamus latiflorus, Phyllostachys nigra, Phyllostachys praecox, Phyllostachys aureosulcata, Phyllostachys pubescens, Phyllostachys atrovittatus, Phyllostachys aurea, and Dendrocalamus giganteus) in southern China are used as raw materials. According to the detection by the relevant departments, the bamboo leaves are rich in flavonoids, bioactive polysaccharides, alkaloids, special amino acids, and trace elements, etc. The flavonoid content of the leaves of D. latiflorus is 1.5%, which is higher than that of other bamboo species. As is known, the leaves of D. latiflorus have been used as food and medicine for a long time in China. According to records in Shennong's Herbal Classic, Famous Physicians' Records, and Compendium of Materia Medica, the leaves of D. latiflorus can clear heart-fire and resolve phlegm, the leaves of D. latiflorus can clear lung and resolve phlegm, and the extract of bamboo leaves is beneficial to human health. This is because the bamboo leaves contain a large amount of flavonoids, bioactive factors, polysaccharides, chlorophyll, and their effective components, such as phenolic acids, anthraquinones, terpenoid lactones, special amino acids, and trace elements such as manganese, zinc, and selenium. The health function factors contained in the bamboo leaves are mainly flavonoid glycosides and coumarin lactones, and the content and bioactivity of their effective components are comparable to those of ginkgo leaves. In fact, the extract of bamboo leaves has excellent effects of resisting free radicals, resisting oxidation, beautifying skin, resisting aging, reducing blood lipids and cholesterol, and has effects of resolving inflammation, resisting bacteria, resisting virus, and improving immunity. The extract of bamboo leaves has a very broad prospect in the field of functional food and Chinese medicine health products and beverages due to its rich resources, excellent quality, proven health effects, fresh and pleasant flavor, and great safety. Other bamboo species such as Phyllostachys sulphonis and Dendrocalamus latiflorus have a very significant health effect on human body due to the large amount of flavonoid polysaccharides and other effective components contained therein. At present, hot water extraction method and microwave extraction method are used to extract flavonoids from bamboo leaves. In China, there are products such as bamboo leaf flavonoid tablets, extracts, and medicine products. There are also beverages prepared by using the extract of D. latiflorus leaves and bamboo shoot juice, such as bamboo leaf wine, bamboo leaf liquor, and bamboo shoot juice beverage.

[0003] Chinese invention patent application: Phyllostachys sulphonis beverage and preparation method thereof (publication number CN1701722A). This technology is to put one or a mixture of several kinds of Phyllostachys sulphonis shoots, stems, leaves, and shoot whips in a common pot to boil, and to obtain the Phyllostachys sulphonis beverage with effects of health care, skin care, refreshing, brain health, lung moistening, and cough relieving.

[0004] There is also a Chinese invention patent application (CN02114071.5) entitled "Lophatherum drink" which uses young bamboo with age below two years, Lophatherum gracile Brongn, glutinous rice, etc. as raw materials, and adopts the following steps: cutting the young bamboo into segments, crushing the Lophatherum gracile Brongn, and then boiling them in water, filtering, adding appropriate amount of protease, honey, and steamed glutinous rice into the filtrate, and then treating by saccharification, filtration, instant high-temperature sterilization, etc. at appropriate temperature. The drink is suitable for people with high blood pressure, high fat, diabetes, and obesity.

[0005] From the above, it can be seen that although people's understanding and interest in the utilization of bamboo resources are increasing and enhancing, the large-scale industrialization of bamboo resources, especially the utilization of bamboo leaf resources, is still not enough. There are more than 30 million mu of green bamboo in China's southern Fujian and Guangdong, combined with other fungal and Chinese herbal medicine materials, to develop more comprehensive health care food and beverages that can serve human health. People and governments hope to have more development of bamboo resources, especially bamboo leaf resources, to "turn waste into treasure" and increase the added value for the three rural economic contributions. The project was evaluated by experts on May 12, 2015. The expert evaluation of the project technical achievements is: "The product of the project mainly uses green bamboo leaves grown in the southern part of China under excellent natural environment, which is rich in bamboo leaf flavonoids. The flavonoids are extracted to make drinks, which have innovation and practicality. The bamboo leaf flavonoid extract is detected by spectrum test, and the flavonoid content is high. SUMMARY

[0006] The purpose of the present application is to provide a method for preparing bamboo leaf flavonoid health care drink and beverage using bamboo leaves of nine high-efficiency bamboo species in southern China as raw materials, which makes the bamboo industry resources and Chinese herbal medicine resources in the south fully developed and utilized, and contributes to the health of human beings, society and the masses.

[0007] The purpose of the present application is achieved by the following technical solutions:

[0008] A health-care drink of flavonoids of bamboo leaves and a bamboo leaf drink are characterized by being prepared from raw materials including the following components by weight: flavonoids and other effective active substances extracted from dry bamboo leaf fragments by a composite enzyme-microwave method, 70 parts; polysaccharides extracted from Hericium erinaceus by a conventional hot water extraction method, 16 parts; effective active substances extracted from Herba Lysimachiae by hot water, 17 parts; and effective active substances extracted from dried Euphorbia neriifolia L. by a continuous countercurrent ultrasonic extraction method, 9 parts; providing Euphorbia neriifolia L. raw materials, grinding the Euphorbia neriifolia L. raw materials to obtain Euphorbia neriifolia L. ground materials; mixing and emulsifying the Euphorbia neriifolia L. ground materials with water to obtain Euphorbia neriifolia L. homogenate liquid, and separating the Euphorbia neriifolia L. homogenate liquid to obtain Euphorbia neriifolia L. separation liquid and Euphorbia neriifolia L. separation residue, 16 parts of which are weighed for standby use; weighing Rhizoma Polygonati, and extracting by hot reflux extraction using ethanol as a solvent to obtain ethanol extraction extract and waste residue, 15 parts of which are weighed for standby use;

[0009] The flavonoids and other effective active substances extracted from dry bamboo leaf fragments by a composite enzyme-microwave method are prepared by the following method: bamboo leaves are made into fragments and washed, then dried and ground into bamboo leaf products with a fineness of 40 mesh, pure water is added, the bamboo leaves and the pure water are added in a weight ratio of 1:15-25, and soaked at 80-100°C, then microwave irradiation is performed at a microwave power of 800W for 5min, then enzymes are added, and the composite enzyme-microwave extracted flavonoids and other effective active substances are obtained by enzymolysis at a pH of 5 and a temperature of 48°C, with enzyme addition amounts of cellulase 0.08-0.10wt%, and pectinase 0.05-0.07wt%;

[0010] The effective active substances extracted from Herba Lysimachiae by hot water are prepared by the following method: the leaves of Herba Lysimachiae are dried and ground into 30-50 mesh, hot water is added, the leaves of Herba Lysimachiae and the hot water are added in a weight ratio of 1:15-25, and cooked at a temperature of 75-85°C, then supercritical extraction is performed by liquid carbon dioxide, and the effective active substances are obtained by filtering and concentrating after 35-40min of extraction;

[0011] The polysaccharides extracted from Hericium erinaceus by a hot water extraction method are prepared by the following method: Hericium erinaceus is weighed, and hot water at 75-95°C is added for extraction to obtain ethanol extraction extract and waste residue, 15 parts of which are weighed for standby use;

[0012] The dried donggareali raw material is weighed, (1) is super-micro ground to a particle size of 80-120 meshes; 500 kg of the powder (the total content of halomonolide and epoxy halomonolide in the raw material is 0.28 wt%) is subjected to continuous countercurrent ultrasonic extraction: 10 times the weight of the raw material of purified water is added, the ultrasonic extraction time is 30 min, the temperature is 20 DEG C, the ultrasonic frequency is 30 KHZ, and the extraction liquid is obtained; (2) the extraction liquid obtained in step (1) is subjected to tubular centrifugation, the centrifugal liquid is subjected to ultrafiltration membrane with a molecular weight of 10,000, and then is transferred to a membrane concentration device, and is concentrated to a solution Brix value of 9.6, and the weight of the obtained concentrated liquid is 86 kg; (3) the extraction liquid obtained in step (2) is subjected to tubular centrifugation, the centrifugal liquid is subjected to ultrafiltration membrane with a molecular weight of 10,000, and then is transferred to a membrane concentration device, and is concentrated to a solution Brix value of 9.6, and the weight of the obtained concentrated liquid is 86 kg; (4) the concentrated liquid obtained in step (3) is transferred to a batching tank, 14.48 kg of alpha-cyclodextrin is added, and homogenization is carried out at room temperature for 20 min to obtain a homogenized liquid; the weight ratio of alpha-cyclodextrin inclusion agent to solute in the concentrated liquid is 2:1; (5) the homogenized liquid obtained in step (4) is subjected to spray drying, and donggareali effective active substance is obtained; the total weight content of halomonolide and epoxy halomonolide in the powder is 6.44% measured by HPLC method, the active ingredient transfer rate is 96.51 wt%, and the product has low bitterness;

[0013] The milkvetch root raw material is weighed and ground to obtain milkvetch root ground material; the milkvetch root ground material is mixed with water and emulsified and stirred to obtain milkvetch root homogenate, and the milkvetch root homogenate is subjected to solid-liquid separation to obtain first ground material; the first ground material is mixed with water in a mass ratio of 1:(4-10) to obtain first finely ground slurry and first finely ground residue precipitated at the bottom of water; the first finely ground residue is subjected to second grinding with water to obtain second finely ground slurry and second finely ground residue precipitated at the bottom of water; the process is repeated for n times, and n is an integer greater than or equal to 2; the finely ground slurries obtained in each step are combined to obtain the milkvetch root ground material; the milkvetch root ground material is mixed with water and subjected to emulsification and stirring, and the mass ratio of the milkvetch root raw material to the water is 1:(10-17); the cycle pressure of emulsification and stirring is 0.1-0.2 MPa, and milkvetch root separation liquid and milkvetch root separation residue are obtained.

[0014] Further, the bamboo leaf flavone health drink and bamboo leaf beverage are characterized in that they further comprise sugar and purified water.

[0015] Further, the added sugar refers to white sugar, fructose syrup, xylitol or stevia leaf sugar.

[0016] Further, the bamboo leaf flavonoids health drink and bamboo leaf beverage are characterized in that dry bamboo leaves are crushed to 40 meshes, appropriately supplemented with water, treated by a microwave frequency of 2450 MHz and a power of 800 W for 5 min; the final material-to-liquid mass ratio is 1:20, cellulase 0.08 wt%, pectinase 0.05 wt% are added at a temperature of 48 DEG C and a pH of 5, and enzymolysis is performed for 2 h, so that the flavonoid extraction rate can reach 90%, which is more than 10% higher than that of a traditional extraction method.

[0017] The application uses the bamboo leaves of 9 high-yield bamboo varieties in southern China as raw materials to prepare a method for preparing a bamboo leaf flavonoid health drink and a bamboo leaf beverage, and is characterized by the following steps: (1) preparing materials: selecting one of the following 9 bamboo species with high flavonoid content: green bamboo, bamboo, black bamboo, Lei bamboo, Huangnaogui bamboo, just bamboo, Xiaoshun bamboo, gold bamboo, and giant dragon bamboo; the bamboo leaves must be sliced on the same day of purchase and dried by sunning or drying; (2) drying the prepared bamboo leaf fragments, washing the bamboo leaf fragments before extraction, and then grinding them into bamboo leaf products with a fineness of 40 mesh, adding purified water, adding bamboo leaves and purified water at a material-to-liquid ratio of 1:15-25, soaking at 80-100°C, and irradiating with a microwave power of 800W for 5min to achieve the best extraction effect; then adding enzymes, and obtaining flavonoids and other active substances by enzymatic hydrolysis at a pH of 5 and a temperature of 48°C, with the enzyme addition amount being cellulase 0.08-0.10% and pectinase 0.05-0.07%; (3) extracting effective components by a fresh and clean big season flower hot water extraction method: drying the leaves of big season flowers, crushing them into 30-50 mesh, adding hot water, adding big season flower leaves and hot water at a material-to-liquid ratio of 1:15-25, and cooking at a temperature of 75-85°C, and then performing supercritical carbon dioxide extraction, filtering and concentrating the active substances for standby use; (4) adding monkey head mushrooms and hot water at a material-to-liquid ratio of 1:15-25 to obtain polysaccharides; (5) weighing dry Donggai Ali raw materials, 1) using ultrafine grinding to a particle size of 80-120 mesh; taking 500kg of powder (the total content of wide pectin and epoxy wide pectin in the raw material is 0.28wt%), and performing continuous countercurrent ultrasonic extraction: adding purified water equivalent to 10 times the weight of the raw material, ultrasonic extraction for 30min at a temperature of 20°C and an ultrasonic frequency of 30KHZ to obtain an extraction liquid; 2) centrifuging the extraction liquid obtained in step 1) by a tubular centrifuge, passing the centrifuged liquid through a ultrafiltration membrane with a molecular weight of 10000, and then transferring it to a membrane concentration device to concentrate it to a solution Brix value of 9.6, and the weight of the concentrated liquid obtained is 86kg; 3) centrifuging the extraction liquid obtained in step 2) by a tubular centrifuge, passing the centrifuged liquid through a ultrafiltration membrane with a molecular weight of 10000, and then transferring it to a membrane concentration device to concentrate it to a solution Brix value of 9.6, and the weight of the concentrated liquid obtained is 86kg; 4) transferring the concentrated liquid obtained in step 3) to a batching tank, adding 14.48kg of α-cyclodextrin, and stirring and homogenizing the mixture at room temperature for 20min to obtain a homogeneous liquid; the weight ratio of α-cyclodextrin inclusion agent to solute in the concentrated liquid is 2:1; 5) spray drying the homogeneous liquid obtained in step 4) to obtain Donggai Ali active substances, and the total weight content of wide pectin and epoxy wide pectin in the powder is 6.44% measured by HPLC, and the active ingredient transfer rate is 96.51wt%, the product has low bitterness; (6) the milk vetch root raw material is weighed and crushed to obtain milk vetch root crushed material; the milk vetch root crushed material is mixed with water and emulsified and stirred to obtain milk vetch root homogenate, and the milk vetch root homogenate is subjected to solid-liquid separation to obtain first crushed material; the first crushed material is mixed with water in a mass ratio of 1: (4-10) to obtain first finely crushed slurry and first finely crushed residue precipitated at the bottom of water; the first finely crushed residue is subjected to second crushing with water to obtain second finely crushed slurry and second finely crushed residue precipitated at the bottom of water; the process is repeated for n times, to obtain nth finely crushed slurry, n being an integer greater than or equal to 2; the finely crushed slurries obtained in each step are combined to obtain the milk vetch root crushed material; the milk vetch root crushed material is mixed with water and subjected to emulsification and stirring, and the mass ratio of the milk vetch root raw material to the water is 1: (10-17); the cycle pressure of emulsification and stirring is 0.1-0.2mpa, to obtain milk vetch root separation liquid and milk vetch root separation residue; (7) the rhizoma polygonati is weighed, ethanol is used as a solvent, and hot reflux extraction is performed to obtain ethanol extraction extract and waste residue, and 15 parts are weighed for standby use.

[0018] The composite enzyme obtained in step (2) - flavonoids and other effective active substances extracted by the microwave method 70 parts, the effective active substances of the hot water extraction of the large season flower obtained in step (3) 17 parts, the polysaccharides extracted by the conventional hot water extraction method of the hericium erinaceus obtained in step (4) 16 parts, the three components are mixed uniformly, then filtered and refined, the filtrate is subjected to instant high-temperature sterilization, hot-filled, and then packaged after cooling to obtain a semi-finished product as an intermediate product of raw liquid, then mixed with the effective active substances of the east gali obtained in step (5) 9 parts, the milk vetch root separation liquid and the milk vetch root separation residue obtained in step (6) 16 parts, and the rhizoma polygonati ethanol extraction extract and waste residue obtained in step (7) 15 parts, and the above components are mixed uniformly at normal temperature and pressure, and then adjusted, constant volume, ultra-high temperature sterilization, hot-filled, and inverted bottle.

[0019] Further, the enzyme in the method is cellulase 0.08%, pectinase 0.05%.

[0020] The beneficial effects of the present application are: (1) the bamboo leaf flavone described in the present application is prepared into health care drinks and beverages, and green bamboo leaves are unique bamboo varieties in southern China, and are known as "green gold, one of the plant kings" in Southeast Asia. According to records in Shennong Herbal Classic, Famous Physicians' Records, Compendium of Materia Medica, Theory of Drug Properties, and Kaibao Herbal Classic, bamboo leaf flavone has the effects of removing heart fire, eliminating inflammation, eliminating internal heat, cooling and detoxifying, and is particularly suitable for eating in smog areas. Bamboo leaves contain 18 kinds of active nutrient components such as bamboo flavones, polysaccharides, polypeptides, dietary fibers, and chlorophyll, and have excellent effects of resisting free radicals, resisting aging, resisting fatigue, reducing blood lipids, preventing cardiovascular and cerebrovascular diseases, promoting memory, improving sleep, beautifying skin, and enhancing immunity. Bamboo leaf flavone also has the functions of antibacterial, antiviral, phlegm relieving, and cough relieving, and the product reaches the international advanced level in color, shape, flavor, and taste. The present application has been searched by the Science and Technology Information Institute, and no literature reports on the use of microwave and composite enzyme to extract bamboo leaf flavone and its industrialized production have been found. The innovation point of the present project is to turn waste into treasure by planting bamboo (agriculture in rural areas, rural areas, and rural areas), and the product has characteristics, is a pure natural gramineous plant, has a competitive advantage in the domestic and foreign markets, gradually occupies a large market share, and has been welcomed and favored by consumers since its advent.

[0021] (2) The present application uses dried Eurycoma longifolia as raw material, which is superfinely pulverized to a particle size of 80-120 mesh, and then subjected to continuous countercurrent ultrasonic extraction of wide-banded ketones, epoxy wide-banded ketones and other effective active substance diterpene components 9 parts. The roots and trunks of the dried Eurycoma longifolia can be used as medicine, which can improve immune function and resist aging, because some components in it can resist free radicals and also have anticancer effect. Some components of Eurycoma longifolia also have the effects of resisting tumors and inhibiting tumors. When the body produces excessive uric acid or the excretion function of uric acid is impaired, the level of uric acid in the blood rises, causing hyperuricemia. Clinically, male blood uric acid higher than 420 μmol / l and female blood uric acid higher than 360 μmol / l are defined as hyperuricemia. The increase of blood uric acid level can lead to the increase of gout prevalence, the production of kidney stones and chronic kidney damage, and is also a high-risk factor for inducing diabetes, hypertension, hyperlipidemia, obesity, cardiovascular disease and other diseases. Hyperuricemia has a high incidence and few treatment drugs, so it is an urgent need to find new uric acid-lowering drugs, and Eurycoma longifolia has broad clinical application prospects. The present application is scientifically formulated with bamboo leaf flavone, and the product prospect is more significant (Green Saint Treasure).

[0022] (3) The present application provides a scientific formulation of bamboo leaf flavone and Eurycoma longifolia raw materials. The Eurycoma longifolia raw material is pulverized to obtain Eurycoma longifolia pulverized material. The Eurycoma longifolia pulverized material is mixed with water and emulsified and stirred to obtain Eurycoma longifolia homogenate liquid. The Eurycoma longifolia homogenate liquid is subjected to solid-liquid separation to obtain Eurycoma longifolia separation liquid and Eurycoma longifolia separation residue. The Eurycoma longifolia separation liquid and the Eurycoma longifolia separation residue are mixed with bamboo leaf flavone to obtain a scientific formulation of bamboo leaf flavone and Eurycoma longifolia.

[0023] (I) As early as the "Essentials of Herbal Properties", it was recorded that it "strengthens tendons and tendons, invigorates blood circulation, nourishes deficiency and moistens the lungs. It treats lower back and leg pain, rheumatic pain, chronic hepatitis, and pulmonary tuberculosis." The "Lu Chuan Materia Medica" also records that it "clears the lungs and stops cough, cools and detoxifies. It treats hemoptysis, dysentery, fever and thirst due to febrile diseases, and dizziness." The combined effects are even better.

[0024] (II) Existing pharmacological studies have shown that *Achyranthes bidentata* extract can effectively scavenge OH and DPPH free radicals, has anti-lipid peroxidation effects, exhibits bidirectional regulatory effects on the proliferation of mouse T lymphocytes, as well as immunomodulatory and hypoglycemic effects. Clinically, it has been proven to have therapeutic effects on various chronic diseases, mainly used to treat lower back pain, leukorrhea due to kidney deficiency, rheumatoid arthritis, lumbar muscle strain, chronic hepatitis, and post-illness weakness.

[0025] (III) This product contains components such as triterpenoids, flavonoids, phenolic glycosides, and lignans. It has extremely high medicinal value, with effects such as nourishing the lungs and replenishing deficiencies, strengthening muscles and tendons, invigorating the body, enhancing immunity and disease resistance, and has broad application and development prospects.

[0026] This invention not only has excellent anti-fatigue properties, but also meets people's demands for safety and environmental friendliness.

[0027] The extract of this product was tested on mice. Experiments including weight-bearing swimming, whole blood lactate content, liver glycogen content, and serum urea nitrogen content were performed on mice after administration. The results showed that the *Smilax glabra* extract provided by this invention has significant anti-fatigue effects. This invention has a simple process, and the resulting extract is a pure natural plant extract that not only has excellent anti-fatigue effects but also meets the safety requirements for human consumption.

[0028] (iv) The application of the *Smilax glabra* extract in the preparation of anti-fatigue drugs or health products.

[0029] This invention relates to an extract of *Achyranthes bidentata* and its applications. The extract is obtained by crushing *Achyranthes bidentata*, placing it in distilled water, heating at 100°C, and then filtering. The residue is then repeatedly extracted with distilled water. The filtrates are combined and concentrated under reduced pressure to obtain an extract, which is then freeze-dried under vacuum to constant weight. This product is scientifically formulated with bamboo leaf flavonoids, resulting in a pure natural plant extract that not only possesses excellent anti-fatigue effects but also meets the demands for safety and environmental friendliness. It can be effectively used in the preparation of anti-fatigue drugs or health products.

[0030] (V) The aforementioned Polygonatum is a traditional Chinese medicine, a plant of the Polygonatum genus, with a horizontal, cylindrical rhizome and enlarged nodules. The leaves are whorled and sessile. Extracts of Polygonatum substances are combined with bamboo leaf flavonoids to create the "Polygonatum Treasure" health drink, whose functions include:

[0031] 1. It is rich in polysaccharides, amino acids, and various nutrients. Its pharmacological functions include:

[0032] ① Antimicrobial effects: In vitro tests show that Polygonatum sibiricum water extract (1:320) has an inhibitory effect on Salmonella typhi, Staphylococcus aureus and acid-fast bacilli. 2% Polygonatum sibiricum in Sabouraud dextrose agar has a different degree of inhibitory effect on common pathogenic fungi.

[0033] ② Effects on blood glucose: When rabbits were administered Polygonatum extract via gavage, their blood glucose levels gradually increased and then decreased. Polygonatum extract showed a significant inhibitory effect on hyperglycemia induced by adrenaline.

[0034] ③ Anti-fatigue effect: Intraperitoneal injection of 0.3 ml / mouse of 17% Polygonatum decoction can prolong the swimming time of mice.

[0035] ④ Antioxidant effect: 20% concentration of Polygonatum decoction, 13ml / mouse, was fed to mice for 27 consecutive days, which increased the activity of superoxide dismutase (SOD) in the liver and decreased the content of lipofuscin in the myocardium.

[0036] ⑤ Anti-aging effect: A 20% concentration of Polygonatum decoction, when used to soak mulberry leaves for feeding silkworms, can prolong the larval stage of silkworms.

[0037] ⑥ Effects on the cardiovascular system: Intravenous injection of 0.16–0.26 g / kg of Polygonatum sibiricum aqueous extract into mice significantly increased coronary blood flow in anesthetized dogs; intravenous injection of 1.5 g / kg antagonized myocardial ischemia induced by posterior pituitary extract in rabbits, counteracted T-wave hyperplasia induced by posterior pituitary extract, and promoted early recovery of abnormal T-waves; intraperitoneal injection of 12 g / kg enhanced the tolerance of mice to hypoxia.

[0038] ⑦ Antiviral effect: 0.2% Polygonatum polysaccharide eye drops, 6 times / day, or Polygonatum polysaccharide 10mg / kg, 2 times / day, have a therapeutic effect on experimental herpes simplex virus keratitis in rabbits. Therefore, the cAMP / cGMP ratio is greater than that of the control group. The cAMP and cGMP contents of spleen tissue are increased, but the cAMP / cGMP ratio does not change significantly.

[0039] The "Huangjingbao" health drink prepared from bamboo leaf flavonoids according to this invention is a colorless or pale yellow, transparent, slightly viscous liquid with a mild and refreshing sweet taste of fructooligosaccharides, free of odor and foreign impurities. It possesses the following effects:

[0040] ① Low calorie value: Because fructooligosaccharides cannot be directly digested and absorbed by the human body, but can only be absorbed and utilized by intestinal bacteria, their calorie value is low, so they will not cause obesity and indirectly have a weight-loss effect. They are also a good sweetener for diabetic patients.

[0041] ②Because it cannot be utilized by oral bacteria, it has the effect of preventing tooth decay.

[0042] ③ The effect of promoting the growth of beneficial intestinal bacteria. Fructooligosaccharides have a selective proliferative effect on beneficial intestinal flora such as Bifidobacteria and Lactobacillus, enabling beneficial flora to dominate in the intestine, inhibiting the growth of harmful bacteria, reducing the formation of toxic substances, and protecting intestinal mucosal cells and liver, thereby preventing the occurrence of diseases and enhancing the body's immunity.

[0043] ④ It can lower the levels of cholesterol and triglycerides in serum.

[0044] ⑤ Promotes nutrient absorption, especially calcium absorption. Ingesting fructooligosaccharides (FOS) can enhance the body's absorption of calcium ions, and several human clinical trials have been conducted. For adolescents, inulin, rich in FOS, has been shown to have a positive effect on bone health by strengthening calcium absorption and increasing bone density. For postmenopausal women, inulin, rich in FOS, has been shown to improve bone health by increasing mineral absorption.

[0045] ⑥ Clinical studies have shown that supplementing with inulin, which is rich in fructooligosaccharides, can help improve mineral absorption in menopausal women and affect their bone turnover markers.

[0046] The effects and efficacy of Hericium erinaceus described in this invention are as follows: ① The polysaccharides extracted from Hericium erinaceus are a mixture of glucans with β1,3 glycosidic bonds in the main chain and β1,6 glycosidic bonds in the side chains; ② As a medicinal fungus, Hericium erinaceus is effective in treating digestive system cancers, ulcers, antral gastritis, chronic gastritis, and other diseases, and can also improve the body's immunity; ③ Medicinal fungal polysaccharides, as an important class of bioactive substances, have shown promise in the treatment of some intractable human diseases. It has shown good therapeutic effects; ④ In recent years, many research methods have been developed for polysaccharide extraction, such as hot water extraction, dilute alkali extraction, and dilute acid extraction; this Hericium erinaceus extract uses hot water extraction; the Hericium erinaceus is weighed according to the ratio and converted to the dry weight, and then extracted with hot water at 75-95℃. Temperatures exceeding 95℃ will affect its activity. Under optimal extraction conditions, the polysaccharide extraction rate is 6.41% by ultrasonic extraction and 5.80% by hot water extraction. The synergistic effect of ultrasound and compound enzyme method can greatly improve the polysaccharide extraction rate. The effective components are extracted using conventional hot water extraction method for later use.

[0047] When the above-mentioned bamboo leaf flavonoid health drink and beverage semi-finished product is used as an intermediate product to make bamboo leaf flavonoid health drink and beverage, an appropriate amount of sugar is added after the above-mentioned extracts are finely filtered, the pH value can be adjusted to 5, and mineral water or purified water in conventional proportions (such as 2T to 5T weight parts) is mixed at a temperature of 48°C, sterilized at high temperature, and then bottled to obtain the finished product.

[0048] Alternatively, when the above-mentioned bamboo leaf flavonoid health drinks and beverage semi-finished products are used as intermediate product raw materials to make bamboo leaf flavonoid health drinks and beverages, an appropriate amount of sugar, a trace amount of malic acid and a conventional weight of purified water are added on the basis of fine filtration of the above-mentioned extracts (before instant high-temperature sterilization), mixed well, sterilized at high temperature and bottled to obtain the finished product.

[0049] The reason for including Hericium erinaceus (monkey head mushroom) as an ingredient in this invention is that Hericium erinaceus is a superior mushroom due to its higher nutritional and medicinal value compared to most other fungi. It is widely used in the food and pharmaceutical industries, possessing a unique flavor and excellent taste. It is a valuable edible and medicinal fungus. The hericium erinaceus used in this invention has the following effects: clearing heat, promoting diuresis, and relieving summer heat. It is used to treat: colds with fever, lung heat cough, damp-heat jaundice, diarrhea and dysentery, urinary tract stones, and prevention of heatstroke. It also has the effect of moisturizing the lungs and detoxifying.

[0050] This invention integrates the nutritional components and other effective ingredients of bamboo leaf flavonoids, Hericium erinaceus, and extracts of *Hypericum perforatum*, creating an excellent bamboo leaf flavonoid health drink and flavonoid beverage. Regular consumption can eliminate various types of reactive oxygen free radicals, inhibit lipid peroxidation, protect cerebral blood vessels, block nitrosation reactions, eliminate nitrosamines, and, like vitamin C, combat fatigue and aging. Bamboo leaf flavonoids are rich in flavonoid compounds and 18 kinds of active nutrients, including bioactive polysaccharides, polypeptides, and chlorophyll, possessing excellent anti-free radical, anti-aging, anti-fatigue, lipid-lowering, cardiovascular disease prevention, memory enhancement, skin beautification, and sleep improvement effects.

[0051] The effects and efficacy of the diterpenoid components of Tongkat Ali ketone and epoxy ketone extracted in this invention, combined with bamboo leaf flavonoids:

[0052] I. Effects on Gout: Hyperuricemia is caused by an imbalance between the production and excretion of uric acid in the body. Uric acid in the body is mainly produced by purine metabolism, and the conversion process is mainly completed by xanthine oxidase. Uric acid excretion can be divided into several processes, including glomerular filtration and renal tubular reabsorption. About two-thirds of uric acid is excreted through the kidneys, which plays a dominant role in uric acid excretion. When there is excessive production of uric acid or impaired uric acid excretion, the uric acid level in the blood rises, causing hyperuricemia. Clinically, hyperuricemia is usually defined as a serum uric acid level higher than 420 μmol / L in men and higher than 360 μmol / L in women. Elevated serum uric acid levels lead to an increased incidence of gout, kidney stones, and chronic kidney damage. It is also a high-risk factor for various diseases such as diabetes, hypertension, hyperlipidemia, obesity, and cardiovascular disease.

[0053] Second, according to Professor Wang Yan, Chief Physician of China-Japan Friendship Hospital, Tongkat Ali has three main effects: 1. Strengthens the kidneys, regulates the intestines and stomach, and nourishes the five internal organs; 2. Promotes development and strengthens the body; 3. Improves the body's immunity.

[0054] Thirdly, according to the preparation method of the present invention, the diterpenoid components in Tongkat Ali can be effectively extracted. 3 kg of Tongkat Ali root slices are weighed, chopped, and extracted three times under reflux with 8 times the volume of 50% etohyl (extraction times are 3 hours, 2 hours, and 2 hours respectively). The three extracts are combined, filtered, and the solvent is recovered under reduced pressure to obtain 175.0 g of extract. 120.0 g of the above extract is extracted with 5.0 L of chloroform / water (1:1, v / v) to obtain 45 g of chloroform extract and 3.0 L of H2O extract. The H2O extract is loaded into an AB-8 macroporous adsorption resin column and eluted sequentially with 20% etohyl, 50% etohyl, and 95% etohyl to obtain 32.4 g of 20% etohyl eluent, 25.8 g of 50% etohyl eluent, and 27.8 g of 95% etohyl eluent. 20.0 g of the above 95% etoh eluent was loaded onto a Sephadex LH-20 column and eluted with meoh-h2O (1:1, v / v). One fraction was collected for every 50 ml, and a total of 20 fractions were collected. The fractions were combined, and the solvent was recovered under reduced pressure to obtain 15.4 g of total diterpenoids (i.e., total diterpenoid components). This provides an effective active substance of Tongkat Ali containing diterpenoid components.

[0055] In this invention, the "Tongkat Ali diterpenoid component" refers to the effective fraction obtained by extracting and purifying Tongkat Ali, which is mainly composed of diterpenoid components.

[0056] The first aspect of this invention provides a Tongkat Ali extract comprising 85% to 95% by weight of Tongkat Ali diterpenoid components. Among these extraction methods, reflux extraction is preferred from the perspective of extraction efficiency. The extraction time is typically at least 15 minutes, preferably at least 30 minutes, and preferably 1 to 3 hours from the perspective of more effectively extracting the diterpenoid components. If the extraction time is less than 15 minutes, the active ingredients cannot be sufficiently extracted; if the extraction time exceeds 3 hours, adverse effects such as decomposition of the active ingredients may occur due to excessive heating time. Detailed Implementation

[0057] In the following examples, the sum of the contents of each component is 100%.

[0058] Example 1

[0059] The following are specific processing examples of bamboo leaf flavonoid health drinks or bamboo leaf beverages prepared according to the present invention. The production and processing steps are as follows:

[0060] (1) Material preparation: Select bamboo leaves from one of the following nine bamboo species: Green bamboo (Horseshoe Green, shoots emerge from May to September, peak shoot season is July to August, it is a sweet bamboo shoot, with abundant leaves and large shoots, 1-1.5 jin / each). There are 3.8 million mu of green bamboo base in Sanming, Fujian, and 13 million mu in southern Fujian. The flavonoid content of the following nine bamboo species is higher than that of other bamboo leaves: Moso bamboo, Black bamboo, Thunder bamboo, Yellow-groove bamboo, Gang bamboo, Xiaoshun bamboo, Golden bamboo, and Giant dragon bamboo. The flavonoid content of green bamboo leaves, Black bamboo, and Giant dragon bamboo leaves is 1.5% higher than that of Moso bamboo leaves. Bamboo leaves must be sliced ​​on the same day they are purchased. The bamboo leaf slices (or chopped) are fragments with a diameter of 1-1.5 cm, which are then sun-dried or oven-dried.

[0061] (2) Selection Steps: Before extraction, the prepared dried bamboo leaf fragments need to be washed, then dried and ground to a fineness of 40 mesh. Add purified water at a weight ratio of 1:20 (material to liquid). Soak at 90℃ for 30 minutes. Microwave irradiation at 800W for 5 minutes yields the best extraction results. Then add enzymes and hydrolyze at pH 5 and 48℃ for 2 hours. The enzyme dosages are: 0.08% cellulase and 0.05% pectinase. The weights of cellulase and pectinase refer to 0.08% and 0.05% of the total weight of the 40-mesh bamboo leaf product and purified water, respectively. The above application of a compound enzyme-microwave method extracts flavonoids, achieving the extraction of flavonoid compounds and other effective active substances from bamboo leaves for later use.

[0062] (3) The method of extracting effective components by hot water extraction of fresh and clean big-flower flowers is as follows: After drying the leaves of big-flower flowers, crush them into 30-50 mesh, add hot water, and cook the big-flower flowers leaves and hot water at a weight ratio of 1:20. Then, supercritical extraction is carried out with liquid carbon dioxide. After dynamic extraction for 38 minutes, filter and concentrate the effective active substances for later use.

[0063] (4) Weigh out the amount of fresh Hericium erinaceus mushrooms according to the specified ratio, converting it to a weight equivalent to the dried product. Method: Hericium erinaceus effects and efficacy: ① The polysaccharides extracted from Hericium erinaceus are a mixture of glucans with β1,3 glycosidic bonds in the main chain and β1,6 glycosidic bonds in the side chains; ② As a medicinal fungus, Hericium erinaceus is effective against digestive system cancers, ulcers, antral gastritis, chronic gastritis, etc., and can improve the body's immunity; ③ Medicinal fungal polysaccharides, as an important class of bioactive substances, play a role in the treatment of some intractable human diseases. The treatment has shown good efficacy; ④ In recent years, many research methods for polysaccharide extraction have been developed, such as hot water extraction, dilute alkali extraction, and dilute acid extraction; this Hericium erinaceus extract uses hot water extraction; the Hericium erinaceus is weighed according to the ratio and converted to dry weight, and then extracted with hot water at 85℃. The ratio of Hericium erinaceus to hot water is 1:20 by weight. Temperatures exceeding 95℃ will affect its activity. Under optimal extraction conditions, the polysaccharide extraction rate is 6.41% with ultrasonic extraction and 5.80% with hot water extraction. The synergistic effect of ultrasound and compound enzyme method can greatly improve the polysaccharide extraction rate. The effective components are extracted using conventional hot water extraction method for later use.

[0064] (5) Ingredients: 70 parts (by weight) of flavonoids extracted from dried bamboo leaf fragments in step (2), 16 parts (by weight) of polysaccharides extracted from Hericium erinaceus using conventional hot water extraction method in step (3), and 17 parts (by weight) of effective active substances extracted from Euphorbia milii using hot water extraction method in step (4) are mixed together, filtered and finely filtered using conventional techniques, and the filtrate is instantaneously sterilized at 120°C for 7 seconds, hot-filled, cooled and packaged as the raw liquid for intermediate products.

[0065] Example 2

[0066] When the semi-finished original liquid of bamboo leaf flavonoid health drink or beverage prepared according to the above-mentioned Example 1 is used as an intermediate product to make bamboo leaf flavonoid health drink or beverage, an appropriate amount of sugar (the amount conventional in the art can be used) is added to the original liquid of the intermediate product of Example 1, the pH value is adjusted to 5, and at a temperature of 48°C, it is mixed with conventional weight ratio (such as 100 kg of the original liquid of Example 1 and 2T to 5T weight of mineral water or purified water), sterilized at high temperature, and bottled to obtain the finished product.

[0067] The "bamboo leaf flavonoid health drink and bamboo leaf" beverage products developed by the present invention have been tested and meet the product Q / GXSP000OIS--2016.

[0068] Bamboo leaves contain approximately 1.5% flavonoids, higher than those in moso bamboo. To fully utilize the unique green bamboo leaf resources of southern China and turn waste into treasure, this invention applies a compound enzymatic hydrolysis combined with microwave processing method to extract the effective components, flavonoids and bioactive polysaccharides, from green bamboo leaves and develop functional bamboo leaf flavonoid health drinks and beverages. The research results will provide a basis for their industrial production. Experiments have shown that this method is simple, quick, energy-efficient, and highly effective.

[0069] 1. Materials and Analytical Methods

[0070] 1.1 Materials and Equipment

[0071] 1.1.1 Raw materials: Green bamboo leaves were provided by Youxi Green Bamboo Cooperative in Fujian Province, Southern China; cellulase (15000r / g) was provided by Wuxi Enzyme Preparation Factory; pectinase (80000r / g) was provided by Tianjin Lihua Enzyme Preparation Factory.

[0072] 1.1.2 Instruments and equipment: PHS-25 pH meter, 722 spectrophotometer, analytical balance, constant temperature water bath, electric plant powder maker, WD800B microwave oven, vacuum filtration system (self-assembled), drying oven, etc.; pilot-scale equipment was provided by Fujian Youxi Kangyuan Health Food Co., Ltd.

[0073] 1.1.3 Reagents: Rutin standard was provided by the National Institutes for Food and Drug Control; other biochemical reagents were of analytical grade and food grade as required.

[0074] 1.2 Analytical Methods

[0075] 1.2.1 Total Flavonoids Determination Method

[0076] 1.2.1.1 Standard Curve Construction: Accurately weigh 10 mg of rutin standard (dried to constant weight at 105℃), place it in a 100 ml volumetric flask, add an appropriate amount of 60% ethanol to dissolve and dilute to the mark, and shake well; accurately measure 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5, 4.0, 4.5, 5.0, 5.5, 6.0, 6.5, 7.0, 7.5, and 8.0 ml, place them in separate 10 ml volumetric flasks, add 60% ethanol to dilute to the mark for each, shake well, and measure the OD value at a wavelength of 265 nm. The reagent is a blank reference. Plot the standard curve.

[0077] 1.2.1.2 Sample Determination: Weigh 3g of fresh bamboo leaves, crush them, and bottle them; soak them in 30mL of petroleum ether for 1h, then filter and dry; evaporate to dryness at 64℃; soak them again in 30mL of petroleum ether for 30min, filter, and evaporate to dryness at 64℃; add 50mL of 75% ethanol, place in a constant temperature water bath at 70℃, and reflux for 1h; repeat the above steps twice more with the filter residue; combine the filtrates, concentrate to 50mL under vacuum at 45℃; add an equal volume of distilled water, mix well, and place in a refrigerator at 4℃ overnight to allow chlorophyll precipitation to be complete; remove the refrigerated liquid, vacuum filter, remove the precipitate, and obtain the crude extract of total flavonoids; make up to 100mL of the crude extract; take 1mL of the crude extract, add 4mL of 30% ethanol and 0.3mL of 5% NaNO2, add 0.3mL of 10% Al(NO)3 after 6min, and add 1mol / L after 6min. Add NaOH to a final volume of 10 mL, and after 15 minutes, the sample extract is ready.

[0078] The OD value of the sample extract was measured at a wavelength of 265 nm. The reagent was used as a blank reference. A standard curve was plotted according to the above. The bamboo juice was filtered by interpolation, and the bamboo juice beverage was vacuum concentrated to obtain bamboo juice extract.

[0079] 2 Results and Analysis

[0080] 2.1 Effect of raw material fineness on extraction rate

[0081] The finer the raw material is ground, the larger its surface area and the better the extraction effect. However, excessively fine grinding will make subsequent separation and filtration difficult. As shown in Table 1, a raw material fineness of 40-60 mesh is ideal. This study selected 40 mesh.

[0082] Table 1. Effect of raw material fineness on extraction efficiency

[0083]

[0084] 2.2 Effect of material-to-liquid ratio on extraction efficiency

[0085] A higher solid-liquid ratio facilitates the diffusion, separation, and dissolution of active ingredients during extraction, resulting in better extraction efficiency. However, an excessively high solid-liquid ratio can also increase the burden on subsequent processes such as filtration and concentration, leading to higher energy consumption. As shown in Table 2, a solid-liquid ratio of 1:20-25 is optimal, and this study selected 1:20.

[0086] Table 2. Effect of solid-liquid ratio on extraction efficiency

[0087] Ratio of material to liquid (W:V) 1:10 1:15 1:20 1:25 Extraction rate (%) 86.0 87.8 89.2 89.3

[0088] 2.3 Selection of Enzymatic Hydrolysis Process Conditions: Enzymatic hydrolysis using cellulase and pectinase. The main factors affecting the enzymatic hydrolysis effect include: enzyme dosage, hydrolysis time, temperature, pH, substrate fineness, and concentration. Among these factors, the research group referred to the optimal pH and temperature for two enzymes provided by the enzyme preparation manufacturer, and obtained the raw material fineness and material-to-liquid ratio through preliminary experiments. The determined optimal conditions were: raw material fineness 40 mesh, material-to-liquid ratio 1:20, pH 5, and temperature 48℃. The research focus was on: enzyme dosage and hydrolysis time.

[0089] 2.3.1 Effect of enzyme dosage on enzymatic hydrolysis efficiency

[0090] Green bamboo leaves contain a relatively high amount of cellulose, hemicellulose, and pectin, which affects the release of effective substances. Direct extraction using traditional methods results in a low extraction rate. This study utilizes cellulase to degrade cellulose in the cell wall, disrupting the cell wall network structure; and pectinase to break down its insoluble protopectin into water-soluble pectic acid, facilitating the release and dissolution of effective substances from the cells. This not only improves the extraction yield but also facilitates subsequent separation and filtration. The total flavonoid content in each extract was then calculated.

[0091] Calculation of total flavonoid extraction rate

[0092] The total flavonoid extraction rate is calculated using the following formula:

[0093]

[0094] Extraction process of total flavonoids from green bamboo leaves

[0095] Fresh green bamboo is washed, dried (60℃), pulverized (40-60 mesh), microwaved, subjected to compound enzymatic hydrolysis, and filtered.

[0096] Table 3 Effect of cellulase dosage on enzymatic hydrolysis efficiency

[0097]

[0098] Table 4. Effect of pectinase dosage on enzymatic hydrolysis efficiency

[0099]

[0100] As can be seen from Tables 3 and 4, under the conditions of raw material fineness of 40 mesh, material-liquid ratio of 1:20, pH 5, 48℃, and 2, the enzyme dosages of 0.08%-0.10% (0.08% in this study) and 0.05%-0.07% (0.05% in this study) of cellulase and pectinase respectively yielded the best extraction results.

[0101] 2.3.2 Effect of enzymatic hydrolysis time on enzymatic hydrolysis efficiency

[0102] Table 5. Effect of enzymatic hydrolysis time on enzymatic hydrolysis efficiency

[0103]

[0104]

[0105] As can be seen from Table 5, under the conditions of raw material fineness of 40 mesh, material-to-liquid ratio of 1:20, pH 5, 48℃, cellulase 0.08%, and pectinase 0.05%, the enzymatic hydrolysis time of 2-2.5h is good. In this study, 2h was used.

[0106] 2.4 Selection of Microwave Processing Conditions

[0107] In recent years, microwave extraction technology has made great progress in the application of food and pharmaceuticals. Although its mechanism is still under investigation, experts agree that the enhanced extraction, dehydration, and sterilization effects of microwaves are caused by their thermal and non-thermal effects. In extraction technology, microwaves work because the extract and the organism contain polar water molecules. Under the influence of a microwave field, these molecules undergo strong polar oscillations, leading to the breakage of hydrogen bonds between cell molecules and the electrical breakdown of cell membranes, promoting matrix permeation and the release of extracted components. Microwave extraction technology is a "green technology" that is short in time, highly efficient, energy-saving, safe, and pollution-free, and can maximize the preservation of the sensory and nutritional value of food. The following experiment demonstrates the selection of optimal microwave power and irradiation time during microwave extraction.

[0108] 2.4.1 Effect of microwave power on extraction rate

[0109] Table 6. Effect of microwave power on extraction rate (extraction time 5 min)

[0110]

[0111] As can be seen from Table 6, high microwave power results in a high extraction rate; 800W was used in this study.

[0112] 2.4.2 Effect of microwave irradiation time on extraction rate

[0113] Table 7. Effect of microwave irradiation time on extraction rate (microwave power 800W)

[0114]

[0115] As can be seen from Table 7, longer microwave irradiation time results in higher extraction rate; the study used 5 minutes.

[0116] 2.5 Comparison of Compound Enzyme-Microwave Extraction Method with Traditional Extraction Method

[0117] Table 8 Comparison of Complex Enzyme-Microwave Extraction Method and Traditional Extraction Method

[0118]

[0119]

[0120] 2.6 Orthogonal Experiment

[0121] 2.6.1 Results and Analysis of Orthogonal Experiments

[0122] According to the orthogonal experimental results, the optimal factor combination is A2B3C2, and the best enzymatic hydrolysis process conditions are: 0.08% cellulose, 0.05% pectinase, hydrolysis temperature of 48℃, and hydrolysis time of 2h.

[0123] Range analysis showed that the order of importance of each factor on the extraction rate was as follows: hydrolysis time > amount > hydrolysis temperature.

[0124] Table 9. Results of the Orthogonal Experiment

[0125]

[0126]

[0127] 3. Conclusion

[0128] The extraction process of flavonoids from green bamboo leaves using the compound enzyme-microwave method, as demonstrated by single-factor and orthogonal experiments, is summarized as follows:

[0129] Dried green bamboo leaves were pulverized through a 40-mesh sieve, and water was added as needed. The mixture was then microwaved at 2450MHz and 800W for 5 minutes. The final material-to-liquid ratio was 1:20. At a temperature of 48℃ and pH 5, 0.08% cellulose and 0.05% pectinase were added, and the mixture was enzymatically hydrolyzed for 2 hours. The flavonoid extraction rate reached about 90%, which is more than 10% higher than that of traditional extraction methods.

[0130] 4. Three batches of pilot production

[0131] Three batches of actual production were carried out using the optimal combination of process conditions to extract flavonoids from green bamboo leaves by compound enzyme-microwave method. The results are shown in Table 10. The extraction rate of the product was 90.24-91.56%, and the extraction effect was good.

[0132] Table 10 Production status of extracting flavonoids from green bamboo leaves using this process.

[0133]

[0134] 5. Development of bamboo leaf flavonoid beverage

[0135] We used green bamboo leaf flavonoids extracted by the compound enzyme-microwave method as raw materials to develop "green bamboo" beverage products, and also produced and formulated bamboo leaf flavonoids with other products.

[0136] 5.1 Production process of "Bamboo Leaf Flavonoids" beverage products

[0137] First, the production process of the "Green Bamboo" beverage product was determined as follows: bamboo leaves, washing, drying, crushing, microwave extraction, enzymatic hydrolysis, coarse filtration, fine filtration, blending, volume adjustment, ultra-high temperature sterilization, hot filling, bottle inversion, spray cooling, labeling, boxing, and finished product (testing).

[0138] 5.2 Production process conditions for "Bamboo Leaf Flavonoids" beverage products

[0139] Bamboo leaves were dried at 60℃ with a moisture content of ≤3% and pulverized through a 40-mesh screen. Microwave extraction was performed with appropriate water replenishment, a microwave power of 800W, and a time of 5 minutes. Enzymatic hydrolysis was conducted with a final material-to-liquid ratio of 1:20, a pH of 5.3, a temperature of 48℃, and a time of 2 hours. Cellulase content was 0.08%, and pectinase content was 0.05%. Coarse filtration was achieved through diatomaceous earth filtration. Fine filtration was performed through microfiltration. Ultra-high temperature sterilization was carried out at 135℃ for 6-8 seconds. Hot filling temperature was ≥85℃. Spraying temperature was ≤40℃.

[0140] 5.3 Formula for "Bamboo Leaf Flavonoids" Beverage Products

[0141] Green bamboo leaves 0.4%, licorice 0.05%, fructose syrup 5%, acesulfame potassium 0.015%, citric acid 0.03-0.05%, ethyl malt 0.001%, edible flavoring as needed, and the rest is mineral water.

[0142] 5.4 "Green Saint Treasure" Beverage Product Formula

[0143] Reserved green bamboo leaves 0.3%, reserve Tongkat Ali 0.3%, Salvia miltiorrhiza 0.1%, Hericium erinaceus 0.2%, red dates 0.03%, Salvia miltiorrhiza 0.1%, the rest is mineral water, hot filling temperature ≥85℃;

[0144] 5.5 "Dali Shuang" Beverage Recipe

[0145] The ingredients include 0.3% green bamboo leaves, 0.4% *Smilax glabra* (a plant in the genus *Smilax*), 0.05% licorice, 5% fructose syrup, 0.03-0.05% citric acid, 0.001% ethyl malt, appropriate amount of edible flavoring, and mineral water.

[0146] 5.6 "Huangjingbao" Health Drink Formula

[0147] A flavonoid from Solomon's seal and bamboo leaves is prepared from the following raw materials in parts by weight: 15 parts green bamboo leaves, 9 parts fructooligosaccharides, 13 parts Solomon's seal, 8 parts kudzu root, 8 parts Hericium erinaceus, and 5 parts egg drop soup.

[0148] A method for preparing Polygonatum odoratum and bamboo leaf extract as described above is as follows:

[0149] 1) First, crush the bamboo in the above weight proportions and pass it through a 40-mesh sieve, then package it in a sterile plastic bag;

[0150] 2) After washing the above-mentioned parts by weight of Polygonatum and Pueraria, place them in a container and crush them with bamboo powder and pass them through a 40-mesh sieve. Then, pack them in a sterile plastic bag and soak them for one hour. After soaking, pour them into a steamer, add water until the water level covers the raw materials, heat the steamer to 100 degrees Celsius and start timing. Maintain the temperature for 30 minutes and then stop heating. After cooling to room temperature, filter to obtain the extract filtrate.

[0151] 3) Place the above-mentioned ingredients in a stainless steel pot, add the extract filtrate from step 2) and 8 parts of drinking water, stir until uniform, and then slowly add drinking water until the total weight is 15 parts, stirring while adding until the color is uniform, to obtain a solution with a weight of 15 parts.

[0152] 4) Add the filtrate extracted in step 2) above, the xylose, fructooligosaccharides and a small amount of other substances in the above weight proportions to a mixer and mix for ten minutes. Then, while stirring, add the binder in step 3) above to obtain a mixture. Adjust the volume, sterilize at ultra-high temperature, hot fill, invert the bottle, spray cool, label, pack, and the finished product. After passing the inspection, it is put into storage.

[0153] Example 3

[0154] A bamboo leaf flavonoid health drink and bamboo leaf beverage, comprising 70 parts of flavonoids and other effective active substances, 16 parts of polysaccharides extracted from Hericium erinaceus using conventional hot water extraction, 17 parts of effective active substances extracted from Euphorbia pekinensis using hot water extraction, and 9 parts of dried Tongkat Ali raw material extracted with ultrafine grinding to a particle size of 80-120 mesh and continuous countercurrent ultrasonic extraction of fennel, epoxy fennel, and other effective active substances; pulverizing the raw material of Achyranthes bidentata to obtain pulverized Achyranthes bidentata; mixing the pulverized Achyranthes bidentata with water and emulsifying and stirring to obtain Achyranthes bidentata homogenate; performing solid-liquid separation on the Achyranthes bidentata homogenate to obtain 16 parts of Achyranthes bidentata separation liquid and Achyranthes bidentata separation residue; weighing Polygonatum sibiricum and using ethanol as solvent for hot reflux extraction to obtain ethanol extract and waste residue, taking 15 parts for later use;

[0155] The flavonoids and other effective active substances are prepared by the following method: bamboo leaves are made into fragments and washed, then dried and ground into bamboo leaf products with a fineness of 40 mesh. Pure water is added, with the ratio of bamboo leaves to pure water being 1:20 by weight. The mixture is soaked at 90°C and then irradiated with microwave power of 800W for 5 minutes. Enzymes are then added, and the mixture is enzymatically hydrolyzed at pH 5 and temperature of 48°C to obtain flavonoids and other effective active substances extracted by the compound enzyme-microwave method. The enzyme dosages are: 0.08wt% cellulose and 0.05wt% pectinase. After 2 hours of enzymatic hydrolysis, the flavonoid extraction rate can reach 90%, which is more than 10% higher than that of traditional extraction methods.

[0156] The effective active substances extracted from the hot water of the large-flowered flower are prepared by the following method: the leaves of the large-flowered flower are dried, crushed into 40 mesh, and hot water is added. The leaves of the large-flowered flower and the hot water are added at a weight ratio of 1:20. The mixture is boiled at 80°C, and then supercritical extraction is performed with liquid carbon dioxide. After 38 minutes of extraction, the effective active substances are filtered and concentrated.

[0157] The polysaccharide extracted from Hericium erinaceus using conventional hot water extraction method is prepared by the following method: weigh Hericium erinaceus, add hot water at 80℃ for extraction, and obtain polysaccharide;

[0158] The dried Tongkat Ali raw material: (1) It is ultra-finely pulverized to a particle size of 100 mesh; 500 kg of powder (the total content of ketone and epoxy ketone in the raw material is 0.28 wt%) is taken and subjected to continuous countercurrent ultrasonic extraction: purified water equivalent to 10 times the weight of the raw material is added, the ultrasonic extraction time is 30 min, the temperature is 20℃, and the ultrasonic frequency is 30 kHz to obtain the extract; (2) the extract obtained in step (1) is centrifuged in a tube, the centrifuged liquid is passed through a 10000 molecular weight ultrafiltration membrane, and then transferred to a membrane concentration device to concentrate to a Brix value of 9.6, and the weight of the concentrate obtained is 86 kg; (3) the extract obtained in step (2) is centrifuged in a tube, and the centrifuged liquid is subjected to continuous countercurrent ultrasonic extraction. The solution was filtered through a 10,000 molecular weight ultrafiltration membrane and then transferred to a membrane concentration device to concentrate it to a Brix value of 9.6. The weight of the concentrate was 86 kg. (4) The concentrate obtained in step (3) was transferred to a mixing tank and 14.48 kg of α-cyclodextrin was added. The mixture was stirred and homogenized at room temperature for 20 min to obtain a homogenized solution. The weight ratio of α-cyclodextrin inclusion agent to solute in the concentrate was 2:1. (5) The homogenized solution obtained in step (4) was spray-dried to obtain 20.98 kg of Tongkat Ali extract powder. The total weight content of ketone and epoxy ketone in the powder was 6.44% by HPLC. The active ingredient transfer rate was 96.51 wt%. The product had a low bitterness.

[0159] The raw material of *Achyranthes bidentata* is crushed to obtain crushed *Achyranthes bidentata*. The crushed *Achyranthes bidentata* is mixed with water and emulsified and stirred to obtain a homogenate of *Achyranthes bidentata*. The homogenate of *Achyranthes bidentata* is subjected to solid-liquid separation to obtain a first crushed material. The obtained first crushed material is mixed with water at a mass ratio of 1:8 to obtain a first fine pulp and a first fine residue that has settled at the bottom of the water. The first fine residue is added with water and crushed a second time to obtain a second fine pulp and a second fine residue that has settled at the bottom of the water. This process is repeated 3 times, and the fine pulps obtained in each step are combined to obtain the crushed *Achyranthes bidentata*. In the step of mixing the crushed *Achyranthes bidentata* with water and emulsifying and stirring, the mass ratio of the raw material of *Achyranthes bidentata* to the water is 1:13; the circulating pressure of emulsification and stirring is 0.1-0.2 MPa.

[0160] The specific preparation method includes the following steps: (1) Material preparation: Select bamboo leaves from one of the following nine bamboo species: green bamboo, moso bamboo, black bamboo, thunder bamboo, yellow bamboo, rigid bamboo, filial bamboo, golden bamboo, and giant dragon bamboo. The flavonoid content of these nine bamboo species is higher than that of other bamboo leaves. The bamboo leaves must be sliced ​​on the same day they are purchased. The bamboo leaf slices or chops are made into fragments with a diameter of 1.3 cm and then dried or oven-dried. (2) Before extraction, the bamboo leaf fragments need to be washed, then dried and ground into bamboo leaf products with a fineness of 40 mesh. Add purified water at a weight ratio of 1:20 (material to liquid). Soak at 90℃ and irradiate with microwave power of 800W for 5 minutes. The extraction efficiency is high. The best results were obtained by adding enzymes and enzymatically hydrolyzing the fruit at pH 5 and 48°C to obtain flavonoids and other effective active substances. The enzyme dosages were: cellulase 0.08% and pectinase 0.05%. (3) The effective components were extracted using the hot water extraction method with fresh and clean *Hylocereus undatus* flowers. The leaves of *Hylocereus undatus* flowers were dried, pulverized into 40 mesh, and hot water was added. The ratio of leaves to hot water was 1:20. The mixture was steamed at 80°C, and then supercritical extraction was performed using liquid carbon dioxide. After 38 minutes of extraction, the effective active substances were filtered and concentrated for later use. (4) *Hericium erinaceus* mushrooms were weighed and extracted with hot water at 75-95°C to obtain polysaccharides. The ratio of leaves to hot water was 1:20. Weight ratio 1:15-25; (5) Weigh the dried Tongkat Ali raw material: 1) Grind it into a particle size of 100 mesh using ultrafine grinding; take 500 kg of powder (the total content of ketone and epoxy ketone in the raw material is 0.28 wt%), and perform continuous countercurrent ultrasonic extraction: add purified water equivalent to 10 times the weight of the raw material, ultrasonic extraction time is 30 min, temperature is 20℃, ultrasonic frequency is 30 kHz, and extract the extract; 2) Centrifuge the extract obtained in step 1) through a tube, pass the centrifuged liquid through a 10000 molecular weight ultrafiltration membrane, and then transfer it to a membrane concentration device to concentrate it to a Brix value of 9.6, and the weight of the concentrated liquid obtained is 86 kg; 3) Take the extract obtained in step 2) The extract was centrifuged in a tube, and the centrifuged liquid was passed through a 10,000 molecular weight ultrafiltration membrane, and then transferred to a membrane concentration device to concentrate the solution to a Brix value of 9.6, resulting in a concentrated solution weighing 86 kg; 4) The concentrated solution obtained in step 3) was transferred to a mixing tank, and 14.48 kg of α-cyclodextrin was added. The mixture was stirred and homogenized at room temperature for 20 min to obtain a homogenized solution; the weight ratio of α-cyclodextrin inclusion agent to solute in the concentrated solution was 2:1; 5) The homogenized solution obtained in step 4) was spray-dried to obtain 20.98 kg of Tongkat Ali extract powder. The total weight content of ketone and epoxyketone in the powder was determined by HPLC to be 6.44%, and the active ingredient transfer rate was 96%.51wt%, the product has low bitterness, take 9 parts of dried Tongkat Ali effective active substances for later use; (6) weigh the raw material of Niu Dali and crush it to obtain Niu Dali crushed material; mix the Niu Dali crushed material with water and emulsify and stir to obtain Niu Dali homogenate, and perform solid-liquid separation on the Niu Dali homogenate to obtain the first crushed material; mix the obtained first crushed material with water at a mass ratio of 1:8 to obtain the first fine pulp and the first fine residue that settles at the bottom of the water; add water to the first fine residue and crush it a second time to obtain the second fine pulp. The second fine residue settled at the bottom of the water; the process is repeated 3 times, and the fine pulp obtained from each step is combined to obtain the crushed *Niu Dali*; in the step of mixing the crushed *Niu Dali* with water and performing emulsification and stirring, the mass ratio of the raw material of *Niu Dali* to the water is 1:13; the circulation pressure of emulsification and stirring is 0.1-0.2 MPa, and *Niu Dali* separation liquid and *Niu Dali* separation residue are obtained for later use; (7) *Huang Jing* is weighed, and ethanol is used as solvent for hot reflux extraction to obtain ethanol extract and waste residue for later use;

[0161] 70 parts of flavonoids and other effective active substances obtained by the compound enzyme-microwave method in step (2) were mixed with 17 parts of effective active substances obtained by hot water extraction of *Hypericum perforatum* obtained in step (3) and 16 parts of polysaccharides obtained by conventional hot water extraction of *Hericium erinaceus* obtained in step (4). After filtration and fine filtration, the filtrate was instantaneously sterilized at high temperature, hot-filled, cooled and packaged as the raw liquid of the intermediate product. Then, 9 parts of effective active substances of Tongkat Ali obtained in step (5) were added and mixed with 16 parts of *Euphorbia pekinensis* separation liquid and *Euphorbia pekinensis* separation residue obtained in step (6) and 15 parts of *Polygonatum sibiricum* ethanol extract and waste residue obtained in step (7). Appropriate amounts of fructose syrup, xylitol or stevia leaf sugar and water were added and mixed evenly. The mixture was prepared, diluted to a certain volume, sterilized at ultra-high temperature, hot-filled, inverted, sprayed and cooled, labeled, packed and finished. After passing the inspection, it was put into storage.

[0162] In conclusion, "every part of the bamboo is valuable," and developing the green bamboo industry is a decision that benefits the country and its people. Green bamboo belongs to the genus *Phyllostachys* of the subfamily Bambusoideae in the family Poaceae. It is a superior bamboo species in southern my country, valued for both its shoots and timber. Because its shoots resemble horseshoes, it is commonly known as "horseshoe bamboo shoots," meaning sweet. It is a top-quality variety, produced in summer and autumn (May to October), with a shoot season lasting more than five months. The shoots are crisp, tender, sweet, and delicious, rich in nutrients, containing 17 essential amino acids, minerals such as phosphorus, iron, and calcium, and vitamins. Green bamboo leaves are rich in flavonoids and bioactive polysaccharides, with a flavonoid content exceeding 1.5%, higher than any other bamboo species. To fully utilize the unique resources of southern China and turn waste into treasure, this research project provides crucial evidence for national industrial production.

Claims

1. A bamboo leaf flavonoid health drink and a bamboo leaf beverage, characterized by the use of... This preparation is made from raw materials comprising the following components in parts by weight: 70 parts of flavonoids and other effective active substances extracted from dried bamboo leaf fragments using a compound enzyme-microwave method; 16 parts of polysaccharides extracted from Hericium erinaceus using a conventional hot water extraction method; 17 parts of effective active substances extracted from Rosa multiflora using hot water extraction; 9 parts of dried Tongkat Ali raw material extracted with ultrafine grinding to a particle size of 80-120 mesh using continuous countercurrent ultrasonic extraction of fennel, epoxy fennel, and other effective active substances; raw material of Achyranthes bidentata is provided, which is then pulverized to obtain pulverized Achyranthes bidentata; the pulverized Achyranthes bidentata is mixed with water and emulsified and stirred to obtain a homogenate of Achyranthes bidentata; the homogenate of Achyranthes bidentata is subjected to solid-liquid separation to obtain Achyranthes bidentata separation liquid and Achyranthes bidentata separation residue, 16 parts of which are weighed for later use; Polygonatum sibiricum is weighed and extracted using ethanol as a solvent by hot reflux to obtain ethanol extract and waste residue, 15 parts of which are weighed for later use; Flavonoids and other active substances extracted from dried bamboo leaf fragments using a compound enzyme-microwave method were prepared by the following method: Bamboo leaves were shredded, washed, dried, and ground into a fine 40-mesh product. Purified water was added at a weight ratio of 1:15-25 (bamboo leaves to purified water). The mixture was soaked at 80-100℃ and then irradiated with 800W microwave power for 5 minutes. Enzymes were then added, and the mixture was enzymatically hydrolyzed at pH 5 and 48℃ to obtain the flavonoids and other active substances extracted using the compound enzyme-microwave method. The enzyme dosages were: cellulase 0.08-0.10 wt% and pectinase 0.05-0.07 wt%. The effective active substances extracted from *Hypericum perforatum* by hot water were prepared by the following method: dried leaves of *Hypericum perforatum* were pulverized into 30-50 mesh, and hot water was added. The leaves and hot water were added at a weight ratio of 1:15-25. The mixture was boiled at a temperature of 75-85℃, and then supercritical extraction was performed using liquid carbon dioxide. After extraction for 35-40 minutes, the mixture was filtered and concentrated to obtain the effective active substances. The polysaccharides extracted from Hericium erinaceus by hot water extraction were prepared by the following method: Hericium erinaceus was weighed, and extracted with hot water at 75-95℃ to obtain an ethanol extract and waste residue. 15 portions were taken for later use. Weigh the dried Tongkat Ali raw material, (1) use ultra-fine grinding to a particle size of 80-120 mesh; take 500 kg of powder (the total content of ketone and epoxy ketone in the raw material is 0.28 wt%), and perform continuous countercurrent ultrasonic extraction: add purified water equivalent to 10 times the weight of the raw material, ultrasonic extraction time is 30 min, temperature is 20℃, ultrasonic frequency is 30KHZ, and extract liquid is obtained; (2) centrifuge the extract obtained in step (1) through a tube, pass the centrifuged liquid through a 10000 molecular weight ultrafiltration membrane, and then transfer it to a membrane concentration device to concentrate it to a solution Brix value of 9.6, and the weight of the concentrated liquid obtained is 86 kg; (3) centrifuge the extract obtained in step (2) through a tube, pass the centrifuged liquid through a 10000 molecular weight ultrafiltration membrane, and then transfer it to a membrane concentration device to concentrate it to a solution Brix value of 9.6, and the weight of the concentrated liquid obtained is 86 kg; (4) take the extract obtained in step (2) through a tube, pass the centrifuged liquid through a 10000 molecular weight ultrafiltration membrane, and then transfer it to a membrane concentration device to concentrate it to a solution Brix value of 9.6, and the weight of the concentrated liquid obtained is 86 kg; (3) The obtained concentrate was transferred to the mixing tank, 14.48 kg of α-cyclodextrin was added, and the mixture was stirred and homogenized at room temperature for 20 min to obtain a homogenized liquid; the weight ratio of α-cyclodextrin inclusion agent to solute in the concentrate was 2:1; (5) The homogenized liquid obtained in step (4) was spray-dried to obtain the effective active substance of Tongkat Ali. The total weight content of ketone and epoxy ketone in the powder was 6.44% by HPLC, the active ingredient transfer rate was 96.51 wt%, and the product had low bitterness; Weigh out the raw material of *Achyranthes bidentata* and crush it to obtain crushed *Achyranthes bidentata*. Mix the crushed *Achyranthes bidentata* with water and emulsify and stir to obtain a homogenate of *Achyranthes bidentata*. Separate the homogenate of *Achyranthes bidentata* to obtain the first crushed material. Mix the obtained first crushed material with water at a mass ratio of 1:(4-10) to obtain the first fine pulp and the first fine residue that settles at the bottom of the water. Add water to the first fine residue and crush it a second time to obtain the second fine pulp and the second fine residue that settles at the bottom of the water. Repeat this process n times to obtain the nth fine pulp, where n is an integer greater than or equal to 2. Combine the fine pulps obtained in each step to obtain the crushed *Achyranthes bidentata*. Mix the crushed *Achyranthes bidentata* with water and emulsify and stir to obtain the raw material of *Achyranthes bidentata* and the water at a mass ratio of 1:(10-17). The circulating pressure of emulsification and stirring is 0.1-0.2 MPa to obtain the separated liquid and separated residue of *Achyranthes bidentata*.

2. The bamboo leaf flavonoid health drink and bamboo leaf beverage according to claim 1, characterized in that: It also includes sugar and purified water.

3. The bamboo leaf flavonoid health drink and bamboo leaf beverage according to claim 2, characterized in that: Added sugars refer to white sugar, high fructose corn syrup, xylitol, or stevia leaf syrup.

4. The bamboo leaf flavonoid health drink and bamboo leaf beverage according to claim 1, characterized in that the dried bamboo leaf is... Bamboo leaves were pulverized through a 40-mesh screen, and water was added as needed. The mixture was then microwaved at 2450MHz and 800W for 5 minutes. The final material-to-liquid mass ratio was 1:

20. At a temperature of 48℃ and pH 5, 0.08wt% cellulose and 0.05wt% pectinase were added, and the mixture was enzymatically hydrolyzed for 2 hours. The flavonoid extraction rate reached 90%, which is more than 10% higher than that of traditional extraction methods.

5. A method for preparing bamboo leaf flavonoid health drinks and bamboo leaf beverages using bamboo leaves from nine high-yield bamboo species in southern China as raw materials, characterized by: The process includes the following steps: (1) Material preparation: Select bamboo leaves from one of the following nine bamboo species: green bamboo, moso bamboo, black bamboo, thunder bamboo, yellow-groove bamboo, rigid bamboo, filial bamboo, golden bamboo, and giant dragon bamboo. The flavonoid content of these nine bamboo species is higher than that of other bamboo leaves. The bamboo leaves must be sliced ​​on the same day they are purchased. The bamboo leaves sliced ​​or chopped into fragments with a diameter of 1-1.5 cm are sun-dried or oven-dried. (2) Before extraction, the bamboo leaf fragments need to be washed, then dried and ground into bamboo leaf products with a fineness of 40 mesh. Add purified water at a weight ratio of 1:15-25 (material to liquid). Soak the bamboo leaves at 80-100℃ and irradiate them with microwave power of 800W for 5 minutes for the best extraction effect. Then, enzymes were added, and flavonoids and other effective active substances were obtained by enzymatic hydrolysis at pH 5 and temperature 48℃. The enzyme dosages were: cellulase 0.08-0.10% and pectinase 0.05-0.07%; (3) The effective components were extracted by hot water extraction of fresh and clean *Heliotropium indicum* flowers: the leaves of *Heliotropium indicum* flowers were dried, pulverized into 30-50 mesh, and hot water was added. The leaves and hot water were added at a weight ratio of 1:15-25. The mixture was steamed at 75-85℃, and then supercritical extraction was performed using liquid carbon dioxide. After extraction for 35-40 minutes, the effective active substances were filtered and concentrated for later use; (4) *Heliotropium indicum* mushrooms were weighed and extracted by soaking in hot water at 75-95℃ to obtain... Polysaccharides, Hericium erinaceus, and hot water were added at a material-to-liquid weight ratio of 1:15-25; (5) Weigh the dried Tongkat Ali raw material, 1) use ultra-fine grinding to a particle size of 80-120 mesh; take 500 kg of powder (the total content of ketone and epoxy ketone in the raw material is 0.28 wt%), and perform continuous countercurrent ultrasonic extraction: add purified water equivalent to 10 times the weight of the raw material, ultrasonic extraction time is 30 min, temperature is 20℃, ultrasonic frequency is 30 kHz, and extract the extract; 2) centrifuge the extract obtained in step 1) through a tube, pass the centrifuged liquid through a 10000 molecular weight ultrafiltration membrane, and then transfer it to a membrane concentration device to concentrate it to a Brix value of 9.6, and the weight of the concentrated liquid is 86 kg. g; 3) Centrifuge the extract obtained in step 2) through a tube, pass the centrifuged liquid through a 10,000 molecular weight ultrafiltration membrane, and then transfer it to a membrane concentration device to concentrate it to a Brix value of 9.6, with a weight of 86 kg; 4) Transfer the concentrate obtained in step 3) to a mixing tank, add 14.48 kg of α-cyclodextrin, and stir and homogenize at room temperature for 20 min to obtain a homogenized liquid; the weight ratio of α-cyclodextrin inclusion agent to solute in the concentrate is 2:1; 5) Spray dry the homogenized liquid obtained in step 4) to obtain the effective active substance of Tongkat Ali. The total weight content of ketone and epoxyketone in the powder was determined by HPLC to be 6.44%, and the active ingredient transfer rate was 96%.51wt%, the product has low bitterness; (6) Weigh the raw material of *Smilax glabra* and crush it to obtain crushed *Smilax glabra*; mix the crushed *Smilax glabra* with water and emulsify and stir to obtain *Smilax glabra* homogenate; perform solid-liquid separation on the homogenate to obtain the first crushed material; The first pulverized material obtained is mixed with water at a mass ratio of 1:(4-10) to obtain the first fine slurry and the first fine residue that settles at the bottom of the water; the first fine residue is added with water for a second pulverization to obtain the second fine slurry and the second fine residue that settles at the bottom of the water; the process is repeated n times to obtain the nth fine slurry, where n is an integer greater than or equal to 2; the fine slurries obtained in each step are combined to obtain the pulverized *Euphorbia lactea*; the pulverized *Euphorbia lactea* is mixed with water and emulsified and shredded, the mass ratio of the *Euphorbia lactea* raw material to the water is 1:(10-17); the circulating pressure of the emulsification and shredding is 0.1-0.2 MPa to obtain the *Euphorbia lactea* separation liquid and the *Euphorbia lactea* separation residue; (7) *Polygonatum sibiricum* is weighed, and ethanol is used as a solvent for hot reflux extraction to obtain ethanol extract and waste residue, and 15 portions are weighed for later use; 70 parts of flavonoids and other effective active substances obtained by the compound enzyme-microwave method in step (2), 17 parts of effective active substances obtained by hot water extraction of *Hypericum perforatum* obtained in step (3), and 16 parts of polysaccharides obtained by conventional hot water extraction of *Hericium erinaceus* obtained in step (4) were mixed evenly. Then, the mixture was filtered and finely filtered. The filtrate was instantaneously sterilized at high temperature, hot-filled, cooled, and packaged as the original liquid of the intermediate product. Then, 9 parts of effective active substances of Tongkat Ali obtained in step (5) were added and mixed. 16 parts of *Euphorbia pekinensis* separation liquid and *Euphorbia pekinensis* separation residue obtained in step (6) were used. 15 parts of *Polygonatum sibiricum* ethanol extract obtained in step (7) were mixed with waste residue. After the mixture was evenly mixed, it was prepared, diluted to a certain volume, sterilized at ultra-high temperature, hot-filled, and inverted.

6. The method according to claim 5, characterized in that: The enzymes were 0.08% cellulase and 0.05% pectinase.

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