High-throughput cell transfection device and application thereof

Abstract

The invention discloses a high-throughput cell transfection device and application thereof, and belongs to the technical field of cell transfection. The device comprises a base table, a transfection disc is arranged at the top of the base table, a plurality of transfection boxes are fixedly connected to the edge of the top of the transfection disc, a liquid discharge port is formed in the middle of the transfection disc, liquid discharge grooves are formed between the liquid discharge port and the transfection boxes, a fixed liquid discharge port is formed in one side of the transfection disc, and the fixed liquid discharge port is connected with the transfection boxes. A butt-joint disc is arranged above the transfection disc, and the center of the bottom of the butt-joint disc is fixedly connected with a movable blocking piece; the liquid level in the transfection box is controlled by adjusting the height of the movable baffle, and a transfection reagent is automatically discharged; compared with a traditional manual transfection reagent replacement mode, the transfection reagent replacement device has the advantages that through the structural design of the transfection disc and the cooperation of the liquid discharging assembly and the liquid adding assembly, automatic discharging and refilling operation of the transfection reagent is achieved, and manpower is saved.

Description

Technical Field

[0001] This invention relates to the field of cell transfection technology, and in particular to high-throughput cell transfection devices and their applications. Background Technology

[0002] Cell transfection is a technique that introduces foreign molecules into eukaryotic cells to alter their genotype or phenotype. The main purpose of transfection is to study gene function or gene products and to influence the production of recombinant proteins by enhancing or inhibiting the expression of specific genes in cells.

[0003] As one of the various cell transfection methods, chemical transfection has advantages such as simple operation, applicability to most adherent and suspension cells, ability to carry large fragments of DNA or RNA, high transfection efficiency, and suitability for in vitro experiments, and is therefore widely used.

[0004] During transfection, the transfection reagent must be changed 4-6 hours after transfection; otherwise, cell death may occur due to the toxicity of the transfection reagent. However, current methods generally require manual medium changes at regular intervals, which consumes a lot of time and effort. In addition, since manual medium changes usually involve opening the transfection box and using a pipette to aspirate and transfer the liquid, the frequent opening of the transfection box and repeated insertion of the pipette into the reagent medium can affect the stability of the cell growth environment, affect cell life activities, and thus affect the transfection throughput. Summary of the Invention

[0005] This invention provides a high-throughput cell transfection device and its application, which can solve the problem that existing operation methods generally require manual medium changes at regular intervals, consuming a lot of time and energy.

[0006] This invention provides a high-throughput cell transfection device, comprising a base, a platform fixedly connected to the center of the base, a transfection disk on the top of the platform, a plurality of transfection boxes fixedly connected to the top edge of the transfection disk, a drain port in the middle of the transfection disk, drain grooves between the drain port and the plurality of transfection boxes, a fixed drain port on the side of the transfection disk near the drain grooves, a drain assembly above the transfection disk, the drain assembly including an adjusting cylinder, a docking plate fixedly connected to the output end of the adjusting cylinder, a plurality of movable baffles fixedly connected to the bottom center of the docking plate, movable drain ports in the middle of the plurality of movable baffles, and clearance grooves at the bottom of the plurality of drain grooves near the transfection disk; a liquid addition assembly for delivering reagents into the transfection disk is provided on one side of the adjusting cylinder.

[0007] As a further aspect of the present invention: a coiled vibrating tube is fixedly connected inside the base, and several vibrating baffles are fixedly connected to the inner wall of the vibrating tube. Both ends of the vibrating tube are connected to an external temperature control and circulation assembly.

[0008] As a further aspect of the present invention: several vibration baffles are divided into a large baffle group and a small baffle group. Several vibration baffles of the large baffle group are fixedly installed on the inner wall of the vibration tube near the inner ring, and several vibration baffles of the small baffle group are fixedly installed on the inner wall of the vibration tube near the edge.

[0009] As a further aspect of the present invention: a closed cover plate is fixedly connected to the edge of the docking plate, and the inner diameter of the closed cover plate is the same as the diameter of the transfection plate; a plurality of sealing sleeves are fixedly connected to the bottom edge of the docking plate, and the diameter of the sealing sleeves is the same as the inner diameter of the transfection box.

[0010] As a further aspect of the present invention: a connecting pipe is fixedly connected to the top of each docking plate, and a sealing sleeve passes through the bottom of each connecting pipe, and the connecting pipe is connected to the output end of the liquid adding component.

[0011] As a further embodiment of the present invention: a three-way solenoid valve is fixedly connected to the top of the connecting pipe, a guide pipe is fixedly connected to one output port of the three-way solenoid valve, the guide pipe is fixedly connected to the output end of the liquid adding component, a pneumatic pipe is fixedly connected to the other output port of the three-way solenoid valve, and a pneumatic pressure regulating component is fixedly connected to one end of the pneumatic pipe.

[0012] As a further aspect of the present invention: the liquid dispensing assembly includes a reagent box, a dispensing pump is fixedly installed inside the reagent box, a dispensing tube is fixedly connected to the output end of the dispensing pump, a flow divider is fixedly connected to the output end of the dispensing tube, the tops of several flow guide tubes are fixedly connected to the bottom of the flow divider, and a flow control valve is fixedly installed in the middle of several flow guide tubes; a dispensing port is opened on one side of the top of the reagent box, and a sealing plug is engaged with the inner wall of the dispensing port.

[0013] As a further aspect of the present invention: the air pressure regulating component includes an air pressure cover, an air pressure detector is fixedly connected to the inner wall of the air pressure cover, an exhaust valve is fixedly installed on one side of the top of the air pressure cover, an air inlet pipe is fixedly connected to one side of the air pressure cover, an air inlet pump is fixedly installed at one end of the air inlet pipe, and an air inlet one-way valve is fixedly installed in the middle of the air inlet pipe.

[0014] As a further embodiment of the present invention: a connector is engaged at the bottom of the drain port, the connector is fixedly installed on the top of the base, a suction tube is fixedly connected to the bottom of the connector, and a drain pump is fixedly installed at one end of the suction tube.

[0015] As a further embodiment of the present invention: a housing is fixedly installed on the top edge of the base, and an installation box is fixedly connected to the top of the housing. The regulating cylinder and the liquid adding component are both disposed in the installation box. A door is rotatably installed on one side of the housing, and an observation window is fixedly installed in the middle of the door.

[0016] The application of high-throughput cell transfection devices includes cell culture equipment and high-throughput cell transfection devices configured on the cell culture equipment. The cell culture equipment is a comprehensive cell and molecular biology laboratory platform composed of existing technologies such as biosafety cabinets, laminar flow hoods, or microscopic observation equipment.

[0017] Compared with the prior art, the beneficial effects of the present invention are as follows: The present invention, by setting a movable baffle that can be raised and lowered, seals and blocks the fixed drain port of the transfection box under normal conditions, ensuring the normal culture environment of the transfection plate. When drainage is required, the height of the movable baffle is adjusted so that its movable drain port corresponds to the fixed drain port, realizing the natural drainage of the liquid in the transfection box under the action of gravity; and because the reagent liquid in the transfection box flows out naturally under the action of gravity, the damage to the cells located at the bottom of the transfection box is minimized during drainage. This invention controls the liquid level in the transfection chamber by adjusting the height of the movable baffle. The lower the height of the movable baffle, the lower the height of its movable drain port, allowing liquid at a lower position to pass through the movable drain port into the drain tank and then be discharged from the drain port. Compared to the traditional method of manually changing transfection reagents, this invention, through the structural design of the transfection tray and the coordination of the drain and add components, achieves automated discharge and refilling of transfection reagents, saving manpower. This invention drives water to circulate along a vibrating tube, and uses the impact of the circulating water on each vibrating plate to generate vibration. The vibration disperses the cells that accumulate on one side of the transfection box during the drainage process, effectively avoiding cell damage and reduced transfection efficiency caused by cell accumulation. At the same time, by adjusting the temperature of the water flow into the vibrating tube, the vibrating tube of this application can meet the needs of temperature control during the transfection process while realizing the vibration function. This invention uses a sealed cover and sealing plug to enclose and protect the environment inside each transfection box. Simultaneously, it utilizes a pressure regulating component to release air when the pressure detected by the pressure gauge exceeds a preset value, and to introduce air when the pressure is below the preset value. This allows cells to be transfected under suitable environmental conditions. Combined with the aforementioned automated medium change operation, the entire transfection process avoids human intervention, maximizing the stability of the cell culture environment, ensuring optimal cell metabolic activity, and thus effectively increasing cell transfection throughput. Attached Figure Description

[0018] Figure 1 This is a schematic diagram of the internal structure of the present invention; Figure 2 This is a schematic diagram of the structure of the transfer disc of the present invention; Figure 3 This is a schematic diagram illustrating the bottom structure of the docking plate according to the present invention; Figure 4 This is a schematic diagram of the top surface structure of the docking plate of the present invention; Figure 5 This is a schematic diagram of the position and structure of the vibration tube of the present invention; Figure 6 This is a cross-sectional view of the vibrating tube of the present invention; Figure 7 This is a cross-sectional structural diagram of the transfection box of the present invention; Figure 8 This is a cross-sectional view of the air pressure regulating component of the present invention; Figure 9 This is a cross-sectional view of the mounting box of the present invention; Figure 10 This is a perspective view of the present invention.

[0019] Explanation of reference numerals in the attached figures: 101. Base; 102. Housing; 103. Door; 104. Observation window; 105. Mounting box; 201. Base; 202. Transfer tray; 203. Transfer box; 204. Drain outlet; 205. Drain trough; 206. Clearance groove; 207. Adjusting cylinder; 208. Movable baffle; 209. Movable drain outlet; 210. Fixed drain outlet; 211. Connecting joint; 301. Connecting plate; 302. Sealing sleeve; 303. Enclosed cover Plate; 304, Flow control valve; 305, Three-way solenoid valve; 306, Air pressure regulating assembly; 3061, Air pressure hood; 3062, Air pressure pipe; 3063, Exhaust valve; 3064, Air inlet pump; 3065, Air inlet pipe; 3066, Air pressure detector; 308, Connecting pipe; 309, Guide pipe; 401, Vibrating pipe; 402, Vibrating baffle; 501, Reagent box; 502, Filling pump; 503, Filling pipe; 504, Sealing plug. Detailed Implementation

[0020] The specific embodiments of the present invention will be described in detail below, but it should be understood that the scope of protection of the present invention is not limited to the specific embodiments.

[0021] like Figures 1 to 10 As shown, this invention provides a high-throughput cell transfection device. Please refer to [link to relevant documentation]. Figure 10 The device includes a base 101, a housing 102 fixedly mounted on the top edge of the base 101, a door 103 rotatably mounted on one side of the housing 102, and an observation window 104 fixedly mounted in the middle of the door 103. The basic external structure of the device, consisting of the base 101, housing 102, and door 103, protects the internal equipment and creates a relatively stable and closed internal environment. The housing 102 is equipped with regulating devices for controlling environmental conditions such as temperature, carbon dioxide, and air pressure. The specific structure of such regulating devices is implemented with reference to existing technologies.

[0022] In one embodiment, see Figure 1 The internal core structure of the device includes a base 201 centrally fixedly connected to the base 101. Please refer to [link / reference]. Figure 2 A transfection tray 202 is snapped onto the top of the abutment 201. Several transfection boxes 203 for cell transfection are fixedly connected to the top edge of the transfection tray 202. A drain port 204 is provided in the middle of the transfection tray 202. Drainage channels 205 are provided between the drain port 204 and each of the transfection boxes 203. A fixed drain port 210 is provided on the side of the transfection tray 202 near the drain channels 205. Please refer to [link / reference]. Figure 3 and Figure 4A drain assembly is provided above the transfection tray 202. The drain assembly includes an adjusting cylinder 207. A mounting box 105 is fixedly connected to the top of the housing 102. The adjusting cylinder 207 is located inside the mounting box 105. A docking plate 301 is fixedly connected to the output end of the adjusting cylinder 207. Several movable baffles 208 are fixedly connected to the bottom center of the docking plate 301. Each of the several movable baffles 208 has a movable drain port 209 in the middle. Please see Figure 7 Several drainage troughs 205 have clearance grooves 206 at their bottom ends near the transfection tray 202. A liquid addition component for supplying reagents into the transfection tray 202 is provided on one side of the adjusting cylinder 207. The bottoms of several movable baffles 208 correspond to the tops of the clearance grooves 206. This application achieves normal sealing of the transfection tray 202 by using movable baffles 208 that can be raised and lowered to block the fixed drainage port 210 on the transfection box 203. The curvature of the movable baffles 208 matches the curvature of the side wall of the transfection box 203, ensuring a stable fit between the movable baffles 208 and the side wall of the transfection box 203, guaranteeing the sealing effect of the fixed drainage port 210 on the transfection box 203. When drainage is required, the height of the movable baffle is adjusted so that the movable drainage port 209 on it aligns with the fixed drainage port 210. The 10-corresponding design enables the natural drainage of reagent liquid inside the transfection box 203. Furthermore, the liquid level inside the transfection box 203 can be controlled by adjusting the height of the movable baffle. That is, the lower the height of the movable baffle, the lower the height of the movable drain port 209, which allows liquid at a lower position to pass through the movable drain port 209 into the drain tank 205 and then be discharged from the drain port 204. Since the reagent liquid inside the transfection box 203 flows out naturally under gravity, it causes less damage to the cells at the bottom of the transfection box 203 during drainage. Compared with the traditional method of manually replacing the transfection reagent every 4 to 6 hours, this application achieves automated drainage and refilling of the transfection reagent through the structural design of the transfection tray 202 and the cooperation of the drainage and filling components, saving manpower.

[0023] In one embodiment, see Figure 2 and Figure 6 The bottom of the drain port 204 is connected to a connector 211, which is fixedly installed on the top of the base 201. The bottom of the connector 211 is fixedly connected to a suction tube, and a drain pump is fixedly installed at one end of the suction tube. By setting up a drain pump and a suction tube, negative pressure suction is used during the drain process to improve the discharge speed of the transfection reagent.

[0024] In one embodiment, during the drainage operation, the reagents in the transfection box 203 flow towards the fixed drainage port 210 for drainage. Therefore, when the liquid level drops to a lower level, the cells at the bottom of the transfection box 203 move with the reagent flow to the side closer to the fixed drainage port 210 and accumulate. This accumulation of cells is prone to damage and affects contact with subsequent transfection reagents and culture media, thus impacting cell viability and transfection efficiency. Please refer to [link to previous text]. Figure 6 and Figure 7 Therefore, this application embeds a vortex-shaped vibrating tube 401 inside the base 201. Several vibrating baffles 402 are fixedly connected to the inner wall of the vibrating tube 401. Both ends of the vibrating tube 401 are connected to an external temperature control and circulation assembly. The external temperature control and circulation assembly drives the water flow to circulate along the vibrating tube 401. The impact of the circulating water flow on each vibrating baffle generates vibration, which disperses the cells accumulated on one side of the transfection box 203, effectively avoiding the problem of cell accumulation. The temperature control and circulation assembly is used to drive the water flow and regulate the water temperature. Its specific structure is implemented with reference to existing technology and should include a drive pump and pipes connected to both ends of the vibrating tube 401. A heater or cooler is installed in the middle of the pipes to help regulate the water temperature. Thus, the vibrating tube 401 of this application can achieve the vibration function while meeting the needs of temperature control during the transfection process.

[0025] In one embodiment, several vibration baffles 402 are divided into a large baffle group and a small baffle group. Several vibration baffles 402 of the large baffle group are fixedly installed on the inner wall of the vibration tube 401 near the inner ring, and several vibration baffles 402 of the small baffle group are fixedly installed on the inner wall of the vibration tube 401 near the edge. This results in greater vibration at the position near the inner ring of the vibration tube 401 (i.e., the side of the transfection box 203 near the fixed drain port 210) and less vibration at the position away from the inner ring of the vibration tube 401 (i.e., the side of the transfection box 203 away from the fixed drain port 210). This achieves the goal of pushing the cells accumulated on the side of the transfection box 203 near the fixed drain port 210 to the other side. In another embodiment, several vibration baffles 402 can be configured to be of different sizes and fixedly connected from the inner ring of the vibration tube 401 to the outer ring of the vibration tube 401 in descending order. Compared with the two-group method of this application, this method results in a more uniform change in vibration force, but it is also more difficult to manufacture.

[0026] In one embodiment, see Figure 4A sealing cover plate 303 is fixedly connected to the edge of the docking plate 301. The inner diameter of the sealing cover plate 303 is the same as the diameter of the transfer plate 202. The sealing cover plate 303 covers and seals the edge of the transfer plate 202, improving the sealing of the working environment of the transfer box 203. Several sealing sleeves 302 are fixedly connected to the bottom edge of the docking plate 301. The diameter of the sealing sleeves 302 is the same as the inner diameter of the transfer box 203. The sealing sleeves 302 are connected to the transfer box 203 to seal and protect the internal environment of the transfer box 203.

[0027] In one embodiment, see Figure 4 To enable the liquid addition operation to the transfection box 203, a connecting pipe 308 is fixedly connected to the top of each docking plate 301. The bottom of each connecting pipe 308 passes through a sealing sleeve 302, and the connecting pipe 308 is connected to the output end of the liquid addition component. Please refer to [link to relevant documentation]. Figure 9 The liquid dispensing assembly includes a reagent tank 501, inside which a dispensing pump 502 is fixedly installed. The output end of the dispensing pump 502 is fixedly connected to a dispensing tube 503, and the output end of the dispensing tube 503 is fixedly connected to a flow divider. The tops of several guide tubes 309 are fixedly connected to the bottom of the flow divider. The guide tubes 309 are flexible tubes. To ensure that the amount of reagent dispensed meets the requirements, a flow control valve 304 is fixedly installed in the middle of several guide tubes 309. A dispensing port is opened on one side of the top of the reagent tank 501. A sealing plug 504 is engaged with the inner wall of the dispensing port so that the reagent in the reagent tank 501 can be replenished after it is consumed.

[0028] In one embodiment, a three-way solenoid valve 305 is fixedly connected to the top of the connecting pipe 308. A guide pipe 309 is fixedly connected to one output port of the three-way solenoid valve 305. The guide pipe 309 is fixedly connected to the output end of the liquid addition component. A pressure pipe 3062 is fixedly connected to the other output port of the three-way solenoid valve 305. A pressure regulating component 306 is fixedly connected to one end of the pressure pipe 3062. By setting the pressure regulating component 306, the cells can be transfected under suitable pressure conditions during the transfection operation.

[0029] In one embodiment, see Figure 8The air pressure regulating component 306 includes an air pressure shroud 3061. An air pressure detector 3066 is fixedly connected to the inner wall of the air pressure shroud 3061. An exhaust valve 3063 is fixedly installed on one side of the top of the air pressure shroud 3061. An air inlet pipe 3065 is fixedly connected to one side of the air pressure shroud 3061. An air inlet pump 3064 is fixedly installed at one end of the air inlet pipe 3065. An air inlet one-way valve is fixedly installed in the middle of the air inlet pipe 3065. The air inlet one-way valve faces the inside of the air pressure shroud 3061. When the air pressure detected by the air pressure detector 3066 exceeds the preset value, the exhaust valve 3063 is opened to exhaust air. When the air pressure detected by the air pressure detector 3066 is lower than the preset value, the air inlet pump 3064 is started to perform an air intake operation. In summary, this application automates the dispensing and filling of transfection reagents, avoiding frequent manual media changes and minimizing cell damage. It also minimizes interference with the cell culture and growth environment. The sealing sleeve 302 and the enclosed cover 303 protect the cell production environment. Combined with the pressure regulating component 306 and the vibration tube 401, the conditions such as cell growth temperature and dispersion within the transfection box 203 are regulated, maximizing the probability of cell death and ensuring normal cell life activities, thereby effectively increasing cell transfection throughput.

[0030] In use, the present invention pre-treats the cells before transfection, allowing the cells to grow in the transfection box 203, and then performs the transfection operation. During the transfection operation, the regulating cylinder 207 first drives the docking plate 301 to press down, and then the sealing cover 303 presses down, so that the sealing cover 303 and the edge of the transfection plate 202 are nested together to achieve the overall sealing of the transfection plate 202. At the same time, the descent of the docking plate 301 drives each movable baffle 208 to press down, so that the movable baffle 208 penetrates into the drain trough 205 and comes into contact with the transfection box 203, and the movable baffle 208 blocks the fixed drain port 210. Then, the transfection reagent is added. Specifically, the dispensing pump is activated, and the reagent is delivered through the dispensing tube 503, the distribution plate, and each guide tube 309. It is then delivered into each transfection box 203 through each connecting tube 308 to transfect the cells. During this process, a circulating temperature control component supplies water to the vibrating tube 401. The water flow within the vibrating tube 401 impacts the vibrating baffle 402, generating vibration. This vibration of the vibrating tube 401 drives the abutment 201 and the transfection plate 202 on it to vibrate, which in turn drives the transfection box 203 to vibrate. This achieves uniform cell dispersion, increasing the contact area between the cells and the transfection reagent, improving transfection throughput, and preventing cell damage due to accumulation. Simultaneously, the circulating temperature control component... The water temperature is adjusted to ensure the reagent temperature inside the transfection box 203 is suitable. After 4-6 hours of static transfection, the drain pump is started, and negative pressure suction is performed through the suction pipe. At the same time, the regulating cylinder 207 drives the docking plate 301 and several movable baffles 208 to descend, so that the movable drain trough 205 is aligned with the fixed drain trough 205. This allows the liquid inside the transfection box 203 to gradually flow into the drain port 204 through the drain trough 205 and be discharged through the suction pipe. After the discharge is completed, the regulating cylinder 207 is started to drive the docking plate 301 and several movable baffles 208 to rise and reset, so that the movable drain trough 205 is misaligned with the fixed drain trough 205. Then, the liquid addition pump is started again to re-inject the transfection reagent into each transfection box 203.

[0031] The above-disclosed embodiments are merely a few specific examples of the present invention. However, the embodiments of the present invention are not limited thereto, and any variations that can be conceived by those skilled in the art should fall within the protection scope of the present invention.

Claims

1. A high-throughput cell transfection device, characterized in that, Includes a base (101), a platform (201) is fixedly connected to the center of the base (101), a transfection tray (202) is provided on the top of the platform (201), a plurality of transfection boxes (203) are fixedly connected to the top edge of the transfection tray (202), a drain port is provided in the middle of the transfection tray (202), a drain groove (205) is provided between the drain port and the plurality of transfection boxes (203), a fixed drain port (210) is provided on the side of the transfection tray (202) near the drain groove (205), and a [missing information] is provided above the transfection tray (202). The system includes a drain assembly comprising an adjusting cylinder (207), the output end of which is fixedly connected to a docking plate (301), and the bottom center of the docking plate (301) is fixedly connected to several movable baffles (208). Each of the movable baffles (208) has a movable drain port (209) in the middle, and each of the several drain troughs (205) has a clearance groove (206) at the bottom of one end near the transfection plate (202). A liquid addition assembly for conveying reagents into the transfection plate (202) is provided on one side of the adjusting cylinder (207).

2. The high-throughput cell transfection device as described in claim 1, characterized in that, The base (201) is fixedly connected to a coiled vibrating tube (401), and a number of vibrating baffles (402) are fixedly connected to the inner wall of the vibrating tube (401). Both ends of the vibrating tube (401) are connected to an external temperature control and circulation assembly.

3. The high-throughput cell transfection device as described in claim 2, characterized in that, Several vibration baffles (402) are divided into large baffle groups and small baffle groups. Several vibration baffles (402) of the large baffle group are fixedly installed on the inner wall of the vibrating tube (401) near the inner ring, and several vibration baffles (402) of the small baffle group are fixedly installed on the inner wall of the vibrating tube (401) near the edge.

4. The high-throughput cell transfection device as described in claim 1, characterized in that, The edge of the docking plate (301) is fixedly connected to a closed cover plate (303), the inner diameter of which is the same as the diameter of the transfection plate (202); a number of sealing sleeves (302) are fixedly connected to the bottom edge of the docking plate (301), the diameter of which is the same as the inner diameter of the transfection box (203).

5. The high-throughput cell transfection device as described in claim 1, characterized in that, The top of each docking plate (301) is fixedly connected to a connecting pipe (308), and the bottom of each connecting pipe (308) passes through a sealing sleeve (302). The connecting pipe (308) is connected to the output end of the liquid adding component.

6. The high-throughput cell transfection device as described in claim 5, characterized in that, A three-way solenoid valve (305) is fixedly connected to the top of the connecting pipe (308). A guide pipe (309) is fixedly connected to one output port of the three-way solenoid valve (305). The guide pipe (309) is fixedly connected to the output end of the liquid adding component. A pneumatic pipe (3062) is fixedly connected to the other output port of the three-way solenoid valve (305). A pneumatic pressure regulating component (306) is fixedly connected to one end of the pneumatic pipe (3062).

7. The high-throughput cell transfection device as described in claim 6, characterized in that, The liquid dispensing assembly includes a reagent box (501), a dispensing pump (502) is fixedly installed inside the reagent box (501), a dispensing tube (503) is fixedly connected to the output end of the dispensing pump (502), a flow divider is fixedly connected to the output end of the dispensing tube (503), the top of several flow guide tubes (309) is fixedly connected to the bottom of the flow divider, and a flow control valve (304) is fixedly installed in the middle of several flow guide tubes (309).

8. The high-throughput cell transfection device as described in claim 6, characterized in that, The air pressure regulating component (306) includes an air pressure hood (3061), an air pressure detector (3066) is fixedly connected to the inner wall of the air pressure hood (3061), an exhaust valve (3063) is fixedly installed on one side of the top of the air pressure hood (3061), an air inlet pipe (3065) is fixedly connected to one side of the air pressure hood (3061), an air inlet pump (3064) is fixedly installed at one end of the air inlet pipe (3065), and an air inlet one-way valve is fixedly installed in the middle of the air inlet pipe (3065).

9. The high-throughput cell transfection device as described in claim 1, characterized in that, The bottom of the drain port is connected to a connector (211), which is fixedly installed on the top of the base (201). A suction pipe is fixedly connected to the bottom of the connector (211), and a drain pump is fixedly installed at one end of the suction pipe.

10. The application of a high-throughput cell transfection device, characterized in that, The invention includes a cell culture device and a high-throughput cell transfection device as described in any one of claims 1-9, wherein the high-throughput cell transfection device is configured on the cell culture device.

Images