Device for non-destructive detachment and collection of cell sheets

Abstract

The application relates to the technical field of cell sheet peeling and collecting, in particular to a cell sheet nondestructive peeling and collecting device, which comprises a rotating shaft, a pushing mechanism, an auxiliary separation mechanism and a separation plate. The processing area and the static area of a processing cylinder are physically isolated through the separation plate. After the separation plate is clamped with a limiting protrusion, the penetration slot is blocked by the baffle to form a closed separation. Compared with the overall rotation of the cylinder in the prior art, only the rotating shaft and the pushing mechanism are operated in the device, the fluid disturbance of the processing area is blocked by the separation plate and cannot be conducted to the static area, the cell sheet of the culture substrate is effectively prevented from being separated due to inertia or disturbance, the accurate distinction between the cell sheet and the suspended cell sheet after peeling is ensured, and the problem of indiscriminate accumulation of the cell sheet is solved.

Description

Technical Field

[0001] This application relates to the technical field of cell sheet peeling and collection, and in particular to a device for non-destructive peeling and collection of cell sheets. Background Technology

[0002] In biomedical engineering applications related to cell sheets, cells before peeling are usually tightly attached to the culture medium bottom in the form of cell sheets containing multiple layers of cells and an intact extracellular matrix. At this time, the culture medium only serves as a carrier to provide nutrition and maintain a suitable environment. The cell sheets maintain a fixed shape and do not disperse or suspend. After non-destructive peeling, the cell sheets will detach from the substrate, and the whole still mainly exists in a sheet-like free aggregate form.

[0003] The peeled cell sheets need to be collected and processed. For example, patent application CN215365697U discloses a cell suspension centrifugation device that can collect the peeled cell sheets. The device includes a mounting base with a rotating seat embedded inside. A toothed disc is fixed to the center of the rotating seat, and one side of the toothed disc meshes with a drive gear. A drive motor is connected below the drive gear. A cylinder is fixed above the toothed disc, and a base is located at the center of the bottom of the cylinder. A bearing seat is connected to the outside of the base, and a pad is fixed above it. A centrifuge seat is located at the center of the pad, and a partition is located above the centrifuge seat. A feed pipe is embedded in the center of the partition. This prior art uses a conical cylinder and the centrifuge seat and blades inside to beat the solution while rotating to accelerate separation. After separation, the solution moves to the feed chamber by inertia, which facilitates the export and extraction of cell suspension.

[0004] The existing technology described above can also be used to collect the peeled cell sheets. However, this technology involves rotating the entire cylinder. While the rotation of the cylinder can move the suspended cell sheets toward the cylinder wall, the cell sheets on the culture medium bottom may also move toward the cylinder wall due to inertia. This causes the cell sheets on the culture medium bottom to detach from the culture medium bottom and float to the surface, eventually accumulating indistinguishably with the peeled cell sheets at the cylinder wall, thus affecting the peeling and collection effect of the cell sheets. Summary of the Invention

[0005] To address the aforementioned technical problems, this application provides a non-destructive cell sheet peeling and collection device, employing the following technical solution: A non-destructive cell sheet peeling and collection device, comprising:

[0006] The processing cylinder is divided into a layered zone, a processing zone, and a static zone from top to bottom.

[0007] A horizontal plate is detachably mounted on top of the processing cylinder.

[0008] A rotating shaft is installed through and rotatably in the middle of the horizontal plate, and is coaxial with the processing cylinder.

[0009] The collection rack is fixed to the bottom of the horizontal plate by symmetrically arranged connecting rods and is located within the layered area.

[0010] A toggle mechanism is located in the section where the rotating shaft is situated in the layered area.

[0011] An auxiliary separation mechanism is located in the part of the rotating shaft that is situated in the processing area.

[0012] A partition plate, located at the bottom of the rotating shaft, is used to separate the processing area from the static area.

[0013] The linkage shaft runs through and is rotatably installed inside the rotating shaft, while the partition plate is rotatably installed at the bottom of the linkage shaft via bearings.

[0014] The partition plate is provided with a through groove, and a fixed ring is fixed at the bottom of the linkage shaft. The fixed ring is provided with a baffle that corresponds to the through groove and can rotate to block the through groove.

[0015] Preferably, the collection rack includes a ring frame and a ring base plate fixed to the bottom of the ring frame. The ring base plate is configured to tilt upward from its sides toward the center to guide the cell sheets to float upward.

[0016] Preferably, the actuating mechanism includes:

[0017] Annular connecting frame, fixed on the rotating shaft

[0018] The actuating plates are arranged in multiples and evenly distributed around the ring frame. The actuating plates are telescopic structures with cavities inside.

[0019] Drive component, used to drive the toggle switch to extend or retract.

[0020] Preferably, the driving component includes:

[0021] The lifting disc is installed on the rotating shaft via a threaded connection.

[0022] Multiple extension rods are provided and fixed to the circumference of the lifting disc.

[0023] There are multiple vertical bars, which are fixed to the partition plate and pass through the corresponding extension bars.

[0024] Multiple linkage rods are provided and fixed to the top of the lifting disc.

[0025] The lifting ring is limited and slidably mounted on the linkage rod.

[0026] The airbag is installed between the lifting ring and the rotating shaft, and is connected to the cavity of the actuating plate.

[0027] Preferably, the telescopic end of the toggle plate is provided with multiple through holes that are connected to the cavity, and a pressure valve is provided on the through holes.

[0028] Preferably, the annular connecting frame is provided with an exhaust pipe, which is connected to an annular transition pipe for blowing air into the area between adjacent toggle plates.

[0029] Preferably, the auxiliary separation mechanism includes:

[0030] The annular limit frame is installed below the lifting disc via an extension rod, and the annular limit frame has an annular groove on its circumference.

[0031] The rotating shaft has multiple blades that are evenly arranged circumferentially within an annular groove.

[0032] The sprocket is mounted on the top of the rotating shaft.

[0033] The retractable linkage shaft has one end connected to any rotating shaft, and the other end passes through and is rotatably mounted on a horizontal plate.

[0034] Preferably, a spacer mesh is provided on the outer side of the annular groove.

[0035] Preferably, the maximum extension length of the actuating plate is less than the distance between the linkage shaft and the rotating shaft.

[0036] Preferably, the inner wall of the processing cylinder is symmetrically provided with limiting protrusions, and the partition plate and the limiting protrusions are interlocked with each other.

[0037] In summary, this application includes at least one of the following beneficial technical effects:

[0038] 1. This device physically isolates the processing area and static area of ​​the processing cylinder through a partition plate. After the partition plate is engaged with the limiting protrusion, the through groove can be sealed by a baffle to form a sealed partition. Compared with the existing technology in which the entire cylinder rotates, this device only rotates the shaft and the actuating mechanism. Fluid disturbance in the processing area is blocked by the partition plate and will not be transmitted to the static area. This effectively prevents the cell sheets at the bottom of the culture medium from detaching due to inertia or disturbance, ensuring accurate differentiation from the suspended cell sheets after peeling, and solving the problem of indiscriminate accumulation of cell sheets.

[0039] 2. This device uses a retractable actuating plate in conjunction with a rotating shaft to replace the overall rotation of the cylinder to guide the cell sheets. The actuating plate adapts to its extension and retraction through airbag inflation, always maintaining effective contact with the cell sheets moving towards the cylinder wall and applying uniform actuating force. At the same time, the rotation speed of the rotating shaft is precisely matched with the extension speed of the actuating plate. Combined with the auxiliary air blowing through the exhaust pipe, this ensures that the suspended cell sheets move efficiently towards the collection area.

[0040] 3. This device, through the through-hole of the actuating plate and its cooperation with the pressure valve, uses airflow compensation to agitate residual cell sheets after the actuating plate reaches its maximum stroke. The annular limiting frame rises to the liquid surface, forming a closed space with the collection area. Combined with the rotating blades disturbing the culture medium, this further pushes the cell sheets towards the collection area. The inclined annular base plate of the collection frame prevents cell sheet accumulation. Finally, collection is completed through drainage and washing, improving the thoroughness and integrity of cell sheet collection. Attached Figure Description

[0041] Figure 1 This is a three-dimensional structural schematic diagram of the present invention.

[0042] Figure 2 This is a schematic diagram of the internal three-dimensional structure of the present invention.

[0043] Figure 3 This is a schematic diagram of the three-dimensional installation structure between the ring frame, the ring base plate, and the partition plate of the present invention.

[0044] Figure 4 This is a bottom view of the partition plate of the present invention.

[0045] Figure 5 This is a schematic diagram of the three-dimensional installation structure between the extension rod, vertical rod, and airbag of the present invention.

[0046] Figure 6 This is the present invention. Figure 5 A magnified view of part A.

[0047] Figure 7 This is a schematic diagram of the three-dimensional installation structure between the extension rod and the annular limiting frame of the present invention.

[0048] Figure 8 This is the present invention. Figure 7 A magnified view of section B.

[0049] Figure 9 This is a schematic diagram of the three-dimensional installation structure between the rotating shaft, the annular connecting frame, and the actuating plate of the present invention.

[0050] Figure 10 This is a cross-sectional view of the internal structure of the toggle plate of the present invention.

[0051] Figure 11 This is the present invention. Figure 10 A magnified view of a portion of point C.

[0052] Explanation of reference numerals in the attached drawings: 1. Processing cylinder; 12. Lifting disc; 13. Extension rod; 14. Vertical rod; 15. Linkage rod; 16. Lifting ring; 17. Airbag; 18. Exhaust pipe; 19. Annular transition pipe; 2. Horizontal plate; 20. Linkage shaft; 3. Rotating shaft; 4. Collection rack; 401. Annular frame; 402. Annular base plate; 5. Connecting rod; 6. Actuating mechanism; 601. Annular connecting frame; 602. Actuating plate; 603. Cavity; 604. Through hole; 7. Auxiliary separation mechanism; 701. Annular limiting frame; 702. Annular groove; 703. Rotating shaft; 704. Rotating blade; 705. Sprocket; 8. Divider plate; 801. Through groove; 9. Linkage shaft; 10. Fixing ring; 11. Baffle. Detailed Implementation

[0053] The following is in conjunction with the appendix Figures 1 to 11 This application will be described in further detail.

[0054] This application discloses a non-destructive cell sheet peeling and collection device, which can collect peeled cell sheets on the culture medium without damaging the cell sheets on the culture medium substrate, and effectively distinguish between peeled and unpeeled cell sheets.

[0055] A cell sheet non-destructive peeling and collection device includes a processing cylinder 1 with its opening facing upwards. The interior of the processing cylinder 1 is divided into a layering zone, a processing zone, and a static zone from top to bottom. A horizontal plate 2 is snapped onto the top of the processing cylinder 1. A rotating shaft 3, coaxial with the processing cylinder 1, is rotatably installed through the middle of the horizontal plate 2. A collection rack 4 is installed inside the layering zone, and the collection rack 4 is installed at the bottom of the horizontal plate 2 via connecting rods 5 symmetrically distributed along the length of the horizontal plate 2.

[0056] The part of the rotating shaft 3 located in the layered area is equipped with a toggle mechanism 6, the part of the rotating shaft 3 located in the processing area is equipped with an auxiliary separation mechanism 7, and a partition plate 8 is provided at the bottom of the rotating shaft 3 to separate the processing area from the static area.

[0057] It should be noted that the culture medium surface inside the processing cylinder 1 is located inside the stratified zone, while the cell sheets at the bottom of the culture medium are located inside the static zone. The peeled cell sheets are suspended on the surface of the culture medium. The processing cylinder 1 is symmetrically provided with limiting protrusions along the length of the horizontal plate 2, and the partition plate 8 slides with the limiting protrusions. The limiting protrusions can limit the partition plate 8 to prevent relative rotation inside the processing cylinder 1. The partition plate 8 can separate the processing zone from the static zone, preventing the cell sheets at the bottom of the culture medium in the static zone from being affected.

[0058] In the initial state, the horizontal plate 2, the rotating shaft 3, and all the components set on them are removed, leaving only the processing cylinder 1. Culture medium is poured into the processing cylinder 1, and a culture medium substrate is set at the bottom of the processing cylinder 1. Cell sheets are inoculated on the culture medium substrate. The workers peel off the cell sheets inside the processing cylinder 1, and the peeled cell sheets are suspended on the surface of the culture medium.

[0059] The processing cylinder 1 has a placement groove that matches the horizontal plate 2. In actual operation, after the cell sheets are peeled off, the horizontal plate 2, the rotating shaft 3, and all the components set on them are placed in the processing cylinder 1, so that the horizontal plate 2 is engaged inside the placement groove, and the partition plate 8 is engaged with the limiting protrusion.

[0060] It should be noted that if the separator plate 8 is a planar and intact structure during the process of entering the culture medium, it will cause the suspended cell sheets to move downward synchronously, thus losing the value of cell sheet collection. Therefore, the separator plate 8 needs to be provided with grooves to make way for the suspended cell sheets. Specifically, a linkage shaft 9 is rotatably installed inside the rotating shaft 3, and the separator plate 8 is rotatably installed at the bottom of the circumference of the linkage shaft 9 through a bearing. Multiple through grooves 801 are evenly opened on the separator plate 8 along its circumference. A fixing ring 10 is installed on the linkage shaft 9 at the bottom of the separator plate 8. Multiple baffles 11 are evenly installed on the fixing ring 10 along its circumference, corresponding one-to-one with the through grooves 801 and used to block the through grooves 801.

[0061] In practice, during the placement of the separator plate 8 into the culture medium, the through groove 801 is kept open so that it can make way for the culture medium as the separator plate 8 moves. During this process, a few peeled cell sheets may still adhere to the bottom of the separator plate 8. At this time, the horizontal plate 2 is gently shaken up and down. The horizontal plate 2, through the cooperation of the rotating shaft 3 and the linkage shaft 9, drives the separator plate 8 to shake slightly. The shaking separator plate 8 can separate the peeled cell sheets from the bottom of the separator plate 8. The separated cell sheets re-enter the culture medium and rise back to the surface of the culture medium.

[0062] When the partition plate 8 moves to the vicinity of the limiting protrusion, the partition plate 8 engages with the limiting protrusion. When the linkage shaft 9 is rotated, the partition plate 8 will not rotate with the linkage shaft 9. The linkage shaft 9 will then drive the baffle 11 to rotate at a certain angle (pre-set) through the fixed ring 10, so that the baffle 11 blocks the through groove 801, thereby separating the treatment area from the static area. This prevents the fluid disturbance caused by the flow of culture medium in the treatment area from causing the culture medium in the static area to flow synchronously, thus ensuring the integrity and adhesion stability of the cell sheet structure attached to the culture medium substrate in the static area.

[0063] The collection rack 4 consists of a ring rack 401, with a ring base plate 402 installed at the bottom of the ring rack 401. The ring base plate 402 is designed to be inclined upward from its side to the center. Thus, when the collection rack 4 enters the culture medium surface, the cell sheets suspended on the culture medium surface can be guided by the ring base plate 402 to be resuspended on the culture medium surface, avoiding the accumulation of suspended cell sheets at the bottom of the ring base plate 402.

[0064] Furthermore, the annular base plate 402 is located inside the culture medium, and the top of the annular frame 401 is located above the surface of the culture medium. The area formed by the annular base plate 402 and the annular frame 401 is set as a collection area, which can collect and process the cell sheets suspended after being moved by the agitator 6.

[0065] The actuating mechanism 6 includes an annular connecting frame 601 mounted on the rotating shaft 3. Multiple actuating plates 602 are evenly arranged on the circumferential surface of the annular connecting frame 601, and the actuating plates 602 are telescopic structures. The area formed by the rotating shaft 3 and the partition plate 8 is also equipped with a driving component for driving the actuating plates 602 to extend and retract.

[0066] In actual operation, after the horizontal plate 2 is placed, the actuating plate 602 is located near the surface of the culture medium (half of the actuating plate 602 is inside the culture medium and half is above the surface of the culture medium). The rotating shaft 3 is driven to rotate by an external drive (motor, etc., not shown in the figure). During the rotation of the rotating shaft 3, the actuating plate 602 is driven to rotate synchronously around its circumference through the annular connecting frame 601. During the rotation of the actuating plate 602, the cell sheets suspended on the surface of the culture medium can be moved towards the wall of the processing cylinder 1 and finally moved into the collection area.

[0067] The rotating shaft 3 and the actuating plate 602 work together to collect the peeled cell sheets, and the separator plate 8 prevents the stability of the cell sheets at the bottom of the culture medium from being affected, thus avoiding the possibility of the cell sheets at the bottom of the culture medium detaching from the culture medium.

[0068] The toggle plate 602 adopts a telescopic structure design, and its length can be elastically extended or rigidly adjusted along its own extension direction.

[0069] During the circumferential rotation of the agitator plate 602 along the rotating shaft 3, when the suspended cell sheets move towards the wall of the processing cylinder 1 under the action of the agitator force, the free end of the agitator plate 602 away from the annular connecting frame 601 can be adaptively extended by external force, always maintaining effective contact with the cell sheets. This ensures that the suspended cell sheets are continuously subjected to a uniform agitator force applied by the agitator plate 602 throughout the entire collection process, avoiding the problem of agitator force attenuation or uneven force due to the gradual increase in the distance between the cell sheets and the rotating shaft 3. This, in turn, ensures the structural integrity of the cell sheets during the collection process and improves collection efficiency.

[0070] The drive assembly designed in this invention includes a lifting disc 12 mounted on a rotating shaft 3 via a threaded connection. Multiple circumferentially distributed extension rods 13 are mounted on the circumferential surface of the lifting disc 12. Multiple vertical rods 14, corresponding one-to-one with the extension rods 13, are mounted on a partition plate 8, with each vertical rod 14 penetrating through its corresponding extension rod. Multiple linkage rods 15 are evenly mounted on the top of the lifting disc 12 along its circumference. A lifting ring 16 is slidably mounted on each linkage rod 15, and an airbag 17 is slidably sleeved on the rotating shaft 3. The end of the airbag 17 furthest from the lifting ring 16 is internally connected to the actuating plate 602.

[0071] The toggle plate 602 has a cavity 603 that is connected to the airbag 17.

[0072] The airbag 17 is a wave-shaped flexible plastic structure that can only deform in the vertical direction and cannot rotate relative to the ground. In actual operation, the rotating shaft 3 drives the lifting disc 12 to rotate during rotation. However, because the vertical rod 14 and the extension rod 13 cooperate to limit the lifting disc 12, the lifting disc 12 cannot rotate but moves upward through the screw thread. Thus, the lifting disc 12 changes from rotation to linear movement along the rotating shaft 3. During the upward movement of the lifting disc 12, the lifting ring 16 moves upward synchronously through the linkage rod 15.

[0073] It should be noted that the top of the lifting ring 16 is provided with an annular groove that cooperates with the linkage rod 15, and the end of the linkage rod 15 away from the lifting disc 12 is limited and slidably disposed inside the annular groove. The linkage rod 15 can only slide horizontally inside the annular groove and cannot move out of the annular groove.

[0074] As the lifting ring 16 moves upward, it causes the airbag 17 to compress. During the compression of the airbag 17, gas is pumped into the cavity 603. The gas causes the telescopic end of the actuating plate 602 to move, so that the telescopic end of the actuating plate 602 is always synchronized with the actuating suspended cell sheet.

[0075] It should be noted that the present invention also includes an airbag 17 assembly and a cavity 603 adapted to the airbag 17. When the device is in the initial working position, the gas inflated by the airbag 17 will fill the sealed area formed by the airbag 17 itself and the interior of the cavity 603.

[0076] In this initial state, the telescopic end of the agitator 602 associated with cell sheet collection remains in its initial contracted state without displacement. This design achieves initial position locking of the telescopic end of the agitator 602 through precise control of the inflation state of the airbag 17, effectively avoiding the following technical problem: that is, before the cell sheets suspended near the agitator 602 enter the effective range of the agitator 602 and before the agitator 602 applies a stable agitating force, the telescopic end of the agitator 602 extends prematurely, resulting in insufficient agitation of the cell sheets by the agitator 602. This leads to the risk that the cell sheets between adjacent agitators 602 cannot be timely and completely agitated to the target collection area, thus providing further assurance for the orderliness and integrity of the cell sheet collection process.

[0077] In addition, to ensure the complete removal of suspended cell sheets between the actuating plates 602, the present invention also provides an exhaust pipe 18 on the annular connecting frame 601 and located at the position of adjacent actuating plates 602. An annular transition pipe 19 is installed on the exhaust pipe 18. In specific operation, when the actuating plate 602 just starts to rotate, gas is pumped into the annular transition pipe 19 by an external air pump. The gas enters the exhaust pipe 18 through the annular transition pipe 19. The gas inside the exhaust pipe 18 is discharged outward, thereby blowing on one side of the wall of the processing cylinder 1 for the suspended cell sheets in the area between adjacent actuating plates 602, assisting in the discharge of the suspended cell sheets in the area between adjacent actuating plates 602, and improving the movement effect of the suspended cell sheets towards the wall of the processing cylinder 1.

[0078] It is necessary to explain that the rotation speed of the rotating shaft 3 is set in advance to ensure that the moving speed of the suspended cell sheet towards the wall of the processing cylinder 1 is matched with the extension speed of the telescopic end of the agitator 602. This avoids the possibility that the extension speed of the telescopic end of the agitator 602 is greater than the moving speed of the suspended cell sheet towards the wall of the processing cylinder 1, which would prevent the agitator 602 from fully agitating the suspended cell sheet towards the wall of the processing cylinder 1.

[0079] The auxiliary separation mechanism 7 includes an annular limiting frame 701, which is installed on the side of the extension rod 13 away from the lifting disc 12. An annular groove 702 is provided on the circumferential surface of the annular limiting frame 701. Multiple rotating shafts 703 are evenly arranged in the circumferential direction inside the annular groove 702. Multiple rotating blades 704 are evenly arranged in the circumferential direction on the circumferential surface of the rotating shaft 703. The rotating shaft 703 passes through the top of the annular limiting frame 701. A sprocket 705 is installed on the part of the rotating shaft 703 located at the top of the annular limiting frame 701. A telescopic linkage shaft 20 is installed on any rotating shaft 703 located directly below the horizontal plate 2. The top end of the linkage shaft 20 is rotatably installed on the horizontal plate 2.

[0080] An interlayer mesh (not shown in the figure) is also provided on the outside of the annular groove 702 to prevent suspended cell sheets from entering the interior of the annular groove 702.

[0081] Before the work begins, all sprockets 705 are connected via existing chains (not shown in the figure). During the work, as the lifting disc 12 moves upward, the extension rod 13 synchronously drives the annular limiting frame 701 to move upward until the annular limiting frame 701 moves to the surface of the culture medium. It should be noted that before this, the cell sheets suspended on the surface of the culture medium have completely entered the collection area. After the annular limiting frame 701 moves to the surface of the culture medium, it forms a closed area with the collection area, preventing the suspended cell sheets from drifting back to the side of the rotating shaft 3 and limiting the suspended cell sheets.

[0082] To further ensure the collection effect of suspended cell sheets in the collection area, the linkage shaft 20 provided by this invention rotates synchronously under the drive of an external driving force (existing motor). During the rotation of the linkage shaft 20, the corresponding rotating shaft 703 is driven to rotate. During the rotation of the rotating shaft 703, the remaining rotating shaft 703 is driven to rotate through the sprocket 705 and chain transmission. During the rotation of the rotating shaft 703, the rotating blade 704 is driven to rotate. During the rotation of the rotating blade 704, the culture medium can be agitated to flow synchronously. The flowing culture medium can further agitate the suspended cell sheets near the annular limiting frame 701 towards the collection area, so as to further ensure the collection effect of the collection area on the suspended cell sheets. The spacer mesh can block the suspended cell sheets and prevent them from entering the annular groove 702.

[0083] The linkage shaft 20 is configured as a telescopic structure that can extend along its length to ensure that the linkage shaft 20 can compensate for the vertical displacement of the annular limit frame 701 while driving the corresponding rotating shaft 703 to rotate, thus preventing the rigid breakage of the linkage shaft 20 when the annular limit frame 701 moves vertically.

[0084] To avoid mechanical collision between the actuating plate 602 and the linkage shaft 20 during the telescopic movement, the present invention limits the telescopic stroke of the actuating plate 602, so that the maximum displacement of the telescopic end of the actuating plate 602 is less than the distance between the linkage shaft 20 and the rotating shaft 3, ensuring that the two do not interfere with each other on the movement trajectory. At the same time, considering that when the telescopic end of the actuating plate 602 reaches the maximum displacement, there is still a gap to be crossed between it and the collection area, if the actuating action of the actuating plate 602 alone cannot cover this displacement, it is easy to cause the cell sheet to be suspended and retained in this area.

[0085] Therefore, this invention provides through holes 604 in the telescopic section of the actuating plate 602. These through holes 604 allow the actuating plate 602 to continue generating a blowing force on the suspended cell sheets even after the actuating plate 602 has passed its maximum displacement. This guides the cell sheets smoothly across the aforementioned spacing area and accurately into the collection area, preventing cell sheet accumulation, damage, or retention, and ensuring the integrity and efficiency of cell sheet collection. Specifically, the telescopic end of the actuating plate 602 has multiple through holes 604 evenly distributed along its height direction, communicating with the cavity 603. Pressure valves are installed on the through holes 604.

[0086] It should be noted that when the actuating plate 602 reaches its maximum extension length, the annular limiting frame 701 has not yet moved to the working position. The extension sections of the actuating plate 602 are sealed, and the culture medium will not enter the cavity 603 through the through hole 604. In actual operation, the rotating shaft 3 needs to continue to rotate to drive the lifting disc 12 to move upward and continue to drive the air bag 17 to expand. The air bag 17 expands and pumps gas into the cavity 603. When the gas inside the cavity 603 reaches the threshold of the pressure valve, the pressure valve opens and releases pressure to the outside through the through hole 604. The directional airflow generated during the pressure release process can form a precise compensating blowing force on the cell sheets that are still in a suspended state outside the maximum extension range of the actuating plate 602, guiding all suspended cell sheets to completely enter the collection area, ensuring the thorough collection of suspended cell sheets.

[0087] Once the suspended cell sheets enter the collection area, the culture medium containing the suspended cell sheets is discharged outward by an existing pump. The collection area is then washed with a culture medium of the same concentration, and finally the washed culture medium is collected, thus collecting the suspended cell sheets.

[0088] Working principle: In the initial state, remove the horizontal plate 2, the rotating shaft 3 and all parts thereof, leaving only the processing cylinder 1. Pour culture medium into the processing cylinder 1, set the culture medium base at the bottom and inoculate cell sheets. After the cell sheets are peeled off, the peeled cell sheets are suspended on the surface of the culture medium. The cell sheets on the culture medium base remain in the static area. Then, snap the horizontal plate 2 into the placement groove at the top of the processing cylinder 1, so that the rotating shaft 3 is coaxial with the processing cylinder 1. The separator plate 8 is engaged with the limiting protrusion inside the processing cylinder 1. The collection rack 4 is fixed to the bottom of the horizontal plate 2 by the connecting rod 5 and is located in the layered area. At this time, the through groove 801 is in the open state. When the separator plate 8 moves down, it makes way for the culture medium through the through groove 801. Slightly shaking the horizontal plate 2 can cause the cell sheets adhering to the bottom of the separator plate 8 to detach and float to the surface of the liquid.

[0089] After the partition plate 8 moves to the limiting protrusion and engages, the limiting protrusion restricts its rotation. The rotating linkage shaft 9 drives the baffle 11 to rotate through the fixed ring 10, so that the baffle 11 blocks the through groove 801 on the partition plate 8, completely separating the treatment area from the static area, and preventing the fluid disturbance in the treatment area from affecting the cell sheets at the bottom of the culture medium in the static area. At this time, the horizontal plate 2 is installed, the actuating mechanism 6 is located in the stratification area, the auxiliary separation mechanism 7 is located in the treatment area, the culture medium surface is in the stratification area, and the suspended cell sheets are located in the culture medium.

[0090] An external drive rotates the rotating shaft 3. Due to the limitation of the vertical rod 14 and the extension rod 13, the lifting disc 12 on the rotating shaft 3 is converted into an upward linear motion through the thread. The lifting ring 16 is moved upward through the linkage rod 15 to compress the air bag 17. The air bag 17 pumps air into the cavity 603 inside the actuating plate 602, so that the telescopic end of the telescopic actuating plate 602 extends out and keeps in contact with the suspended cell sheet. The rotating shaft 3 drives the annular connecting frame 601 and the actuating plate 602 to rotate, pushing the liquid cell sheet towards the wall of the processing cylinder 1. At the same time, the external air pump supplies air to the exhaust pipe 18 between the adjacent actuating plates 602 through the annular transition pipe 19 to assist the cell sheet in moving towards the cylinder wall. The rotation speed of the rotating shaft 3 ensures that the moving speed of the cell sheet matches the extension speed of the actuating plate 602.

[0091] When the actuating plate 602 reaches its maximum extension length, the lifting disc 12 continues to drive the extension rod 13 upward, causing the annular limiting frame 701 to rise to form a closed area with the culture medium surface and the collection area. The rotating shaft 3 continues to drive the airbag 17 to expand. When the air pressure in the cavity 603 reaches the pressure valve threshold, the pressure valve opens and releases pressure through the through hole 604. The airflow blows the suspended cell sheets that have not reached the collection area. At the same time, the linkage shaft 20 drives the rotating shaft 703 to rotate. Through the sprocket 705 and chain drive, all rotating shafts 703 operate synchronously. The rotating blades 704 agitate the culture medium, pushing the suspended cell sheets near the annular limiting frame 701 towards the collection area. Finally, the culture medium containing the suspended cell sheets is discharged through the existing pump body. After washing the collection area with culture medium of equal concentration, the washing liquid containing the suspended cell sheets is collected, completing the cell sheet collection.

[0092] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the invention can be implemented in other specific forms without departing from its spirit or essential characteristics. Therefore, the embodiments should be considered in all respects as exemplary and non-limiting, and the scope of the invention is defined by the appended claims rather than the foregoing description. Thus, all variations falling within the meaning and scope of equivalents of the claims are intended to be included within the present invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

[0093] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A device for non-destructive peeling and collection of cell sheets, characterized in that, include: The processing cylinder is divided into a layered zone, a processing zone, and a static zone from top to bottom. A horizontal plate is detachably mounted on top of the processing cylinder; A rotating shaft is installed through and rotatably in the middle of a horizontal plate, and is coaxial with the processing cylinder; The collection rack is fixed to the bottom of the horizontal plate by symmetrically arranged connecting rods and is located within the layered area; A toggle mechanism is located on the portion of the rotating shaft situated in the layered area; An auxiliary separation mechanism is located in the part of the processing area where the rotating shaft is situated. A partition plate, located at the bottom of the rotating shaft, is used to separate the processing area from the static area; A linkage shaft is installed inside the rotating shaft, and a partition plate is installed at the bottom of the linkage shaft via a bearing. The partition plate is provided with a through groove, and a fixed ring is fixed at the bottom of the linkage shaft. The fixed ring is provided with a baffle that corresponds to the through groove and can rotatably block the through groove. The inner wall of the processing cylinder is symmetrically provided with limiting protrusions, and the partition plate is engaged with the limiting protrusions; The collection rack includes a ring frame and a ring base plate fixed to the bottom of the ring frame. The ring base plate is configured to tilt upward from its sides toward the center to guide the cell sheets to float upward. The actuating mechanism includes: an annular connecting frame fixed to a rotating shaft; multiple actuating plates evenly distributed circumferentially on the annular connecting frame, the actuating plates being telescopic with internal cavities; and a driving assembly for driving the actuating plates to extend and retract, including: a lifting disc threadedly mounted on the rotating shaft; multiple extension rods fixed to the circumference of the lifting disc; multiple vertical rods fixed to a partition plate and passing through corresponding extension rods; multiple linkage rods fixed to the top of the lifting disc; a lifting ring slidably mounted on the linkage rods; and an airbag installed between the lifting ring and the rotating shaft, communicating with the cavity of the actuating plate. The auxiliary separation mechanism includes: an annular limiting frame, which is installed below the lifting disc via an extension rod, and the annular limiting frame has an annular groove on its circumferential surface; a rotating shaft, which has multiple rotating blades evenly arranged in the annular groove; a sprocket, which is installed at the top of the rotating shaft; and a telescopic linkage shaft, one end of which is connected to any rotating shaft, and the other end of which passes through and is rotatably installed on the horizontal plate.

2. The cell sheet non-destructive peeling and collection device according to claim 1, characterized in that, The telescopic end of the toggle plate is provided with multiple through holes that are connected to the cavity, and pressure valves are provided on the through holes.

3. The cell sheet non-destructive peeling and collection device according to claim 1, characterized in that, An exhaust pipe is provided on the annular connecting frame, and the exhaust pipe is connected to an annular transition pipe for blowing air into the area between adjacent toggle plates.

4. The cell sheet non-destructive peeling and collection device according to claim 1, characterized in that, A spacer mesh is provided on the outside of the annular groove.

5. The cell sheet non-destructive peeling and collection device according to claim 1, characterized in that, The maximum extension length of the actuating plate is less than the distance between the linkage shaft and the rotating shaft.

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