An apparatus and method for measuring hydrogen peroxide content

Abstract

This invention relates to the field of hydrogen peroxide measurement, and discloses a device and method for measuring hydrogen peroxide content. The device includes a liquid absorption component, a gaseous absorption component, and a solid absorption component, used to collect hydrogen peroxide in different forms to obtain a test liquid, which is then separated into a first test liquid and a second test liquid. The solid absorption component includes a first gas-liquid separation chamber and a vapor trap, connected to a first three-way valve and a second three-way valve. A first fluorescence measurement channel is used to detect and obtain first fluorescence detection data for the first test liquid; a second fluorescence measurement channel is used to detect and obtain second fluorescence detection data for the second test liquid after hydrogen peroxide removal; a data processing module is used to compare and process the first and second fluorescence detection data to obtain hydrogen peroxide content data. This application can collect hydrogen peroxide in various forms, thereby enabling a more comprehensive and accurate measurement of hydrogen peroxide in the ambient atmosphere.

Description

Technical Field

[0001] This invention relates to the field of hydrogen peroxide measurement, and in particular to a device and method for measuring the content of hydrogen peroxide. Background Technology

[0002] Hydrogen peroxide is an important oxide in the troposphere, possessing high reactivity and oxidizing power. It plays a crucial role in ozone formation within the troposphere, thus its concentration can be considered an indicator of atmospheric oxidation. Hydrogen peroxide is also a significant factor contributing to acid deposition and increased secondary aerosol concentrations, impacting ecosystems and climate change. Furthermore, due to its strong oxidizing properties, hydrogen peroxide depletes the body's antioxidant capacity, potentially causing DNA damage leading to gene mutations and various diseases. Long-term exposure to high concentrations of hydrogen peroxide can irritate the eyes, respiratory tract, and skin. Therefore, when hydrogen peroxide levels in the air exceed a certain threshold, it adversely affects plant and microbial growth, as well as human health. Accurate monitoring of hydrogen peroxide levels in ambient air and at pollution sources is therefore crucial for public health.

[0003] Currently, the analysis and measurement of hydrogen peroxide in the ambient atmosphere mainly considers hydrogen peroxide existing in gaseous form. Therefore, when collecting and testing hydrogen peroxide in the ambient atmosphere, the focus is mainly on collecting and testing gaseous hydrogen peroxide. However, in reality, hydrogen peroxide in the ambient atmosphere is not limited to gaseous form. For example, hydrogen peroxide may also exist as solid particles. Hydrogen peroxide existing in other forms besides gaseous is often easily overlooked in the test, which can easily lead to insufficient accuracy in the measurement results of hydrogen peroxide content in the atmospheric environment. Summary of the Invention

[0004] The purpose of this invention is to provide a device and method for measuring hydrogen peroxide content, which can more comprehensively measure hydrogen peroxide in the atmospheric environment and improve the accuracy and reliability of the measurement results.

[0005] To solve the above-mentioned technical problems, the present invention provides a hydrogen peroxide content measuring device, comprising:

[0006] The liquid absorption assembly is used to collect dissolved hydrogen peroxide in the sample solution to obtain the test liquid;

[0007] A gaseous absorption component is used to absorb gaseous hydrogen peroxide from the sample gas to obtain the liquid to be tested.

[0008] A solid-state absorption assembly is used to collect dissolved hydrogen peroxide in a sample solution to obtain a test liquid. The solid-state absorption assembly includes a first gas-liquid separation chamber and a vapor trap, and is connected to a first three-way valve and a second three-way valve. The output end of the gas-state absorption assembly is connected to the input end of the first three-way valve. The first output end of the first three-way valve is connected to the input end of the first gas-liquid separation chamber. The gas output end of the first gas-liquid separation chamber is connected to the input end of the vapor trap. The second output end of the first three-way valve, the liquid output end of the first gas-liquid separation chamber, and the output end of the vapor trap are all connected to the first input end of the second three-way valve. The output end of the liquid absorption assembly is connected to the second input end of the second three-way valve. The output end of the second three-way valve is connected to the input ends of both the first and second fluorescence measurement channels, so as to separate the test liquid output from the second three-way valve into a first test liquid and a second test liquid.

[0009] The first fluorescence measurement channel is used to detect and obtain the first fluorescence detection data of the first test solution;

[0010] The second fluorescence measurement channel is used to detect and obtain the second fluorescence detection data of the second test solution after removing hydrogen peroxide;

[0011] The data processing module is used to compare and process the first fluorescence detection data and the second fluorescence detection data to obtain hydrogen peroxide content data.

[0012] In one optional embodiment of this application, the gaseous absorption assembly includes a water bath temperature-controlled trapping spiral tube and an absorption liquid container connected to the first input end of the water bath temperature-controlled trapping spiral tube via a peristaltic pump; the absorption liquid container is used to store an absorption liquid whose main component is potassium hydrogen phthalate buffer solution.

[0013] The second input end of the water bath temperature-controlled trapping spiral tube is connected to the gas collection port;

[0014] The input end of the first three-way valve is connected to the output end of the water bath temperature control and trapping spiral tube.

[0015] In one optional embodiment of this application, the output end of the second three-way valve is connected to a second gas-liquid separation chamber; the liquid output end of the second gas-liquid separation chamber is connected to both the first fluorescence measurement channel and the input end of the second fluorescence measurement channel.

[0016] In one optional embodiment of this application, it further includes a standard liquid container, a cleaning liquid container, a third three-way valve, and a fourth three-way valve;

[0017] The output end of the absorption liquid container is connected to the first input end of the third three-way valve, and the output end of the standard liquid container is connected to the second input end of the third three-way valve; the output end of the third three-way valve is connected to the first input end of the fourth three-way valve through a peristaltic pump.

[0018] The output end of the cleaning fluid container is connected to the second input end of the fourth three-way valve; the output end of the fourth three-way valve is connected to the first input end of the water bath temperature control and trapping spiral tube.

[0019] In an optional embodiment of this application, a fifth three-way valve and a peroxide removal device are further provided between the water bath temperature-controlled trapping spiral tube and the gas collection port;

[0020] The gas collection port is connected to the first input terminal of the fifth three-way valve via a peristaltic pump; the first output terminal of the fifth three-way valve is connected to the second input terminal of the water bath temperature-controlled trapping spiral tube; the second output terminal of the fifth three-way valve is connected to the input terminal of the peroxide removal device; and the output terminal of the peroxide removal device is connected to the second input terminal of the water bath temperature-controlled trapping spiral tube.

[0021] In one optional embodiment of this application, the first fluorescence measurement channel includes a first spiral tube, a first water bath temperature control spiral tube, a first pH adjustment spiral tube, and a first fluorescence detection chamber connected in sequence.

[0022] The second fluorescence measurement channel includes a second spiral tube, a second water bath temperature-controlled spiral tube, a second pH adjustment spiral tube, and a second fluorescence detection chamber connected in sequence.

[0023] The input ends of the first and second spiral tubes are used to receive the first test solution and the second test solution, respectively; and the input end of the first spiral tube is connected to a first liquid container via a peristaltic pump; the first liquid container is used to store potassium hydrogen phthalate buffer solution; the input end of the second spiral tube is connected to a second liquid container via a peristaltic pump; the second liquid container is used to store potassium hydrogen phthalate buffer solution containing hydrogen peroxide catalytic enzyme.

[0024] The input ends of the first water bath temperature-controlled spiral tube and the second water bath temperature-controlled spiral tube are respectively connected to a third liquid container via a peristaltic pump. The third liquid container is used to store a mixture of puerarin and peroxidase.

[0025] The input ends of the first pH adjusting spiral tube and the second pH adjusting spiral tube are respectively connected to pH adjusting liquid containers via peristaltic pumps;

[0026] The data processing module is connected to the first fluorescence detection chamber and the second fluorescence detection chamber respectively, and is used to compare the first fluorescence detection data measured in the first fluorescence detection chamber and the second fluorescence detection data measured in the second fluorescence detection chamber to obtain hydrogen peroxide content data.

[0027] In one optional embodiment of this application, the first liquid container, the second liquid container, the third liquid container, and the pH adjustment liquid container are all brown spout bags.

[0028] In an optional embodiment of this application, an air venting tee is provided between the first spiral tube and the first water bath temperature-controlled spiral tube, between the first water bath temperature-controlled spiral tube and the first pH adjustment spiral tube, between the first pH adjustment spiral tube and the first fluorescence detection chamber, between the second spiral tube and the second water bath temperature-controlled spiral tube, between the second water bath temperature-controlled spiral tube and the second pH adjustment spiral tube, and between the second pH adjustment spiral tube and the second fluorescence detection chamber.

[0029] A method for measuring hydrogen peroxide content, applied to the hydrogen peroxide content measuring device as described in any of the preceding claims, comprising:

[0030] The gaseous hydrogen peroxide in the sample gas to be tested is absorbed by the gaseous absorption component and / or the hydrogen peroxide particles in the sample gas to be tested are absorbed by the solid absorption component and / or the dissolved hydrogen peroxide in the sample liquid to be tested is collected by the liquid absorption component to obtain the sample liquid to be tested;

[0031] The liquid to be tested is divided into a first test liquid and a second test liquid;

[0032] The first test liquid is subjected to fluorescence detection through the first fluorescence measurement channel to obtain first fluorescence detection data;

[0033] Hydrogen peroxide is removed from the second test solution through the second fluorescence measurement channel, and fluorescence detection is performed on the second test solution after removing hydrogen peroxide to obtain second fluorescence detection data;

[0034] The hydrogen peroxide content is obtained by comparing and calculating the first fluorescence detection data and the second fluorescence detection data.

[0035] In one optional embodiment of this application, a process for pre-calibrating the concentration response curve is also included;

[0036] The process of pre-calibrating the concentration response curve includes:

[0037] Prepare standard solutions of hydrogen peroxide at different concentrations;

[0038] Each concentration of the hydrogen peroxide standard solution is divided into two portions and passed into the first fluorescence measurement channel and the second fluorescence measurement channel respectively to obtain the first calibration detection data and the second calibration detection data corresponding to each different concentration of the hydrogen peroxide standard solution.

[0039] The calibration test data of the hydrogen peroxide standard solution is obtained by performing a difference calculation on the first calibration test data and the second calibration test data corresponding to the same concentration of hydrogen peroxide standard solution.

[0040] A concentration response curve is obtained by linearly fitting the calibration detection data corresponding to the hydrogen peroxide standard solutions of different concentrations and the hydrogen peroxide concentration.

[0041] This invention provides a hydrogen peroxide content measuring device and method. The hydrogen peroxide content measuring device includes a liquid absorption component for collecting dissolved hydrogen peroxide in a sample liquid to obtain a test liquid; a gaseous absorption component for absorbing gaseous hydrogen peroxide in a sample gas to obtain a test liquid; and a solid absorption component for collecting dissolved hydrogen peroxide in the sample liquid to obtain a test liquid. The solid absorption component includes a first gas-liquid separation chamber and a vapor trap, and is connected to a first three-way valve and a second three-way valve. The output end of the gaseous absorption component is connected to the input end of the first three-way valve. The first output end of the first three-way valve is connected to the input end of the first gas-liquid separation chamber. The gas output end of the first gas-liquid separation chamber is connected to the input end of the vapor trap. The first three-way valve... The second output terminal, the liquid output terminal of the first gas-liquid separation chamber, and the output terminal of the vapor trap are all connected to the first input terminal of the second three-way valve; the output terminal of the liquid absorption assembly is connected to the second input terminal of the second three-way valve; the output terminal of the second three-way valve, the input terminal of the first fluorescence measurement channel, and the input terminal of the second fluorescence measurement channel are all connected to separate the test liquid output from the output terminal of the second three-way valve into a first test liquid and a second test liquid; the first fluorescence measurement channel is used to detect and obtain the first fluorescence detection data of the first test liquid; the second fluorescence measurement channel is used to detect and obtain the second fluorescence detection data of the second test liquid after removing hydrogen peroxide; the data processing module is used to compare and process the first fluorescence detection data and the second fluorescence detection data to obtain hydrogen peroxide content data.

[0042] This application provides a detection device for detecting hydrogen peroxide content, which includes a gaseous absorption component, a solid absorption component, and a liquid absorption component. The solid absorption component mainly comprises a first gas-liquid separation chamber and a vapor trap. The first gas-liquid separation chamber separates the liquid to be tested and the gaseous portion containing particulate matter from the gas-liquid mixture output from the gaseous absorption component. The vapor trap further dissolves and absorbs the particulate matter containing hydrogen peroxide. Thus, by cooperating with the gaseous absorption component, the solid absorption component, and the liquid absorption component, it is possible not only to collect gaseous hydrogen peroxide in the air, but also to absorb and collect particulate solid hydrogen peroxide, and to collect and measure hydrogen peroxide dissolved in liquids such as rainwater through the liquid absorption component. This application collects hydrogen peroxide from multiple different dimensions, thereby more comprehensively and accurately measuring different forms of hydrogen peroxide in the ambient atmosphere and improving the accuracy and reliability of hydrogen peroxide content measurement. Furthermore, during the hydrogen peroxide content measurement process, two fluorescence measurement channels are utilized. Two test liquids containing hydrogen peroxide from the same source are divided into two portions. One portion of the test liquid is directly subjected to fluorescence detection in the first fluorescence measurement channel, obtaining the first fluorescence detection data. The other portion of the test liquid is first subjected to fluorescence detection in the second fluorescence measurement channel to remove the hydrogen peroxide, obtaining the second fluorescence detection data as a reference. Based on the comparison between the first and second fluorescence detection data, interference from other peroxides besides hydrogen peroxide on the measurement results can be effectively eliminated, further improving the accuracy and reliability of hydrogen peroxide content measurement. Attached Figure Description

[0043] To more clearly illustrate the technical solutions of the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0044] Figure 1 A schematic diagram of the frame structure of the hydrogen peroxide content measuring device provided in the embodiments of this application;

[0045] Figure 2 A schematic flowchart illustrating the hydrogen peroxide content measurement method provided in this application embodiment;

[0046] Figure 3 A schematic diagram showing the particulate matter content of air after passing through a water bath temperature-controlled trapping spiral tube, as provided in an embodiment of this application.

[0047] Figure 4 A schematic diagram showing the content of particulate matter remaining after being absorbed by a steam trap in the air, as provided in an embodiment of this application.

[0048] Figure 5 This is a schematic diagram showing the hydrogen peroxide content measured in air with and without hydrogen peroxide particles, provided in an embodiment of this application.

[0049] Figure 6 A schematic diagram illustrating the particulate matter absorption rate of steam at different temperatures in a steam trap provided in this application embodiment;

[0050] Figure 7 Another schematic flowchart of the hydrogen peroxide content measurement method provided in the embodiments of this application;

[0051] In the attached diagram: 100 is a peristaltic pump, 10 is a gaseous absorption assembly, 11 is a water bath temperature-controlled trapping spiral tube, 12 is a peroxide removal device, 200 is a bubble-removing tee, 20 is a solid absorption assembly, 21 is the first gas-liquid separation chamber, 22 is a vapor trap, 30 is a liquid absorption assembly, 40 is the first fluorescence measurement channel, 41 is the first spiral tube, 42 is the first water bath temperature-controlled spiral tube, 43 is the first pH adjustment spiral tube, 44 is the first fluorescence detection chamber, 50 is the second fluorescence measurement channel, 51 is the second spiral tube, and 52 is... 53 is the second water bath temperature control spiral tube, 54 is the second pH adjustment spiral tube, 55 is the second fluorescence detection chamber, 60 is the data processing module, 71 is the first three-way valve, 72 is the second three-way valve, 73 is the third three-way valve, 74 is the fourth three-way valve, 75 is the fifth three-way valve, 81 is the absorption liquid container, 82 is the standard liquid container, 83 is the cleaning liquid container, 84 is the first liquid container, 85 is the second liquid container, 86 is the third liquid container, 87 is the pH adjustment liquid container, 9 is the second gas-liquid separation chamber, 91 is the filter membrane, and 92 is the air pump. Detailed Implementation

[0052] The core of this invention is to provide a device and method for measuring hydrogen peroxide content, which can collect and measure hydrogen peroxide in different forms, thereby improving the accuracy and reliability of hydrogen peroxide measurement results.

[0053] To enable those skilled in the art to better understand the present invention, the invention will be further described in detail below with reference to the accompanying drawings and specific embodiments. Obviously, the described embodiments are merely some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0054] like Figure 1 As shown, Figure 1 This is a schematic diagram of the frame structure of the hydrogen peroxide content measuring device provided in the embodiments of this application.

[0055] In one specific embodiment of this application, the hydrogen peroxide content measuring device may include:

[0056] Liquid absorption component 30, gas absorption component 10, solid absorption component 20, first fluorescence measurement channel 40, second fluorescence measurement channel 50 and data processing module 60;

[0057] The gaseous absorption component 10 is used to absorb gaseous hydrogen peroxide in the sample gas to obtain the test liquid; the solid absorption component 20 is used to absorb hydrogen peroxide particles in the sample gas to obtain the test liquid; the liquid absorption component is used to collect the test liquid with dissolved hydrogen peroxide to obtain the test liquid; the output terminals of the gaseous absorption component 10, the solid absorption component 20, and the liquid absorption component 30 are all connected to the input terminals of the first fluorescence measurement channel 40 and the second fluorescence measurement channel 50, so as to separate the test liquid into the first test liquid and the second test liquid;

[0058] The first fluorescence measurement channel 40 is used to perform fluorescence detection on the first test solution to obtain first fluorescence detection data; the second fluorescence measurement channel 50 is used to remove hydrogen peroxide from the second test solution and perform fluorescence detection on the second test solution after removing hydrogen peroxide to obtain second fluorescence detection data.

[0059] The data processing module 60 is used to compare and process the first fluorescence detection data and the second fluorescence detection data to obtain hydrogen peroxide content data.

[0060] In this embodiment, the components for absorbing and collecting hydrogen peroxide in the analyte include a gaseous absorption component 10, a solid absorption component 20, and a liquid absorption component 30. Taking the measurement target as the hydrogen peroxide content in the air as an example, the collected air can be used as the sample gas. The gaseous absorption component 10 is mainly used to absorb gaseous hydrogen peroxide in the air, while the solid absorption component 20 is mainly used to absorb solid particulate hydrogen peroxide in the air. In the current conventional testing process where the measurement target is the hydrogen peroxide content in the air, the hydrogen peroxide content in the air is mainly measured directly using air as the detection object. The measured data is mainly gaseous hydrogen peroxide in the air, and solid particulate hydrogen peroxide is easily overlooked because it is not specifically collected. In this embodiment, a solid absorption component 20 is added to specifically collect and absorb solid particulate hydrogen peroxide in the air, which can improve the comprehensiveness and reliability of hydrogen peroxide measurement to a certain extent.

[0061] This embodiment further considers that in humid conditions or rainy weather, hydrogen peroxide in the air inevitably dissolves in dew or rainwater. The condensed dew and rainwater can absorb hydrogen peroxide from the air to some extent. Therefore, measuring the hydrogen peroxide content in dew and rainwater can compensate for the inadequacy of simply measuring the gaseous hydrogen peroxide content in the air as the overall atmospheric hydrogen peroxide content. Based on this, this embodiment also includes a liquid absorption component 30, which can specifically be a collection device for collecting dew or rainwater. The liquid absorption component 30 can be an open container with an open top, and multiple adsorption rods or plates can be installed inside the container. This allows for efficient absorption of dew while maintaining the open container's ability to absorb rainwater.

[0062] It is understood that the hydrogen peroxide content measuring device in this application is not limited to measuring the hydrogen peroxide content in air and rainwater. In practical applications, it can also be used to measure the hydrogen peroxide content in other gases or liquids or even solid substances (measured after being formed into powder). This application does not specifically limit this.

[0063] like Figure 1 As shown, the gaseous absorption component 10 in this embodiment is a device for absorbing and collecting gaseous hydrogen peroxide in the sample gas to be measured. When the sample gas to be measured is air, a potassium hydrogen phthalate buffer solution can be used as the absorbent for absorbing hydrogen peroxide. Therefore, in an optional embodiment of this application, the gaseous absorption component 10 mainly includes a water bath temperature-controlled trapping spiral tube 11. In addition, the first input end of the water bath temperature-controlled trapping spiral tube 11 is connected to an absorbent container 81 through a peristaltic pump 100. The absorbent container 81 is used to store the absorbent solution with potassium hydrogen phthalate buffer solution as the main component. The second input end of the water bath temperature-controlled trapping spiral tube 11 is connected to a gas collection port.

[0064] In this embodiment, the water bath temperature-controlled trapping spiral tube 11 is a fluid channel with a spiral tube structure, allowing the sample gas and absorbent to be fully mixed within the spiral tube structure. Furthermore, circulating water is wrapped around the spiral tube, and this circulating water, while maintaining circulation, is also controlled by a temperature control device to a set constant temperature. The temperature of the circulating water in the water bath temperature-controlled trapping spiral tube 11 of this embodiment can be controlled between 4℃ and 10℃ to ensure good absorption performance of the absorbent for peroxides. In addition, the absorbent stored in the absorbent container 81 of this embodiment can be obtained by diluting potassium hydrogen phthalate buffer solution; the preparation process of this potassium hydrogen phthalate buffer solution is as follows:

[0065] Weigh 41g of potassium hydrogen phthalate, add 185ml of 1mol / L NaOH solution and 4L of deionized water and mix well. Measure the pH value of the solution with a pH meter, and adjust the pH value to 5.8~6.0 with 1mol / L NaOH solution or concentrated HCl solution. Then add 100mg of EDTA (ethylenediaminetetraacetic acid) and 1ml of 37% formaldehyde solution to obtain potassium hydrogen phthalate buffer solution.

[0066] In subsequent embodiments, the potassium hydrogen phthalate buffer solution is a solution with the same composition and concentration as the solution obtained by the above preparation method, which will not be repeated in this application.

[0067] In this embodiment, the absorbent can be obtained by diluting 0.5L of potassium hydrogen phthalate buffer solution with 4.5L of deionized water. The absorbent container 81 can be a brown nozzle bag, which can better avoid environmental interference. The brown nozzle bag can be stored in a heat preservation device at 4℃~10℃ to ensure the good working performance of the absorbent.

[0068] Therefore, when ambient air is introduced into the water bath temperature-controlled trapping spiral tube 11, the absorbent in the absorbent container 81 can be drawn into the water bath temperature-controlled trapping spiral tube 11 by the peristaltic pump 100. The water bath temperature of the water bath temperature-controlled trapping spiral tube 11 can be controlled between 4℃ and 10℃. Thus, at the water bath temperature of 4℃ to 10℃, the absorbent and air are fully mixed and contacted in the water bath temperature-controlled trapping spiral tube 11, so that the gaseous hydrogen peroxide in the air can be absorbed by the absorbent to the greatest extent.

[0069] Based on this, since both the solid absorption component 20 and the gaseous absorption component 10 absorb hydrogen peroxide in the sample gas, the solid absorption component 20 can be placed at the output end of the gaseous absorption component 10. That is to say, the gas-liquid mixture output from the water bath temperature-controlled trapping spiral tube 11 can be further absorbed by the solid absorption component 20 for hydrogen peroxide solid particles.

[0070] It should be noted that although the spiral tube in the water bath temperature-controlled collecting spiral tube 11 can fully mix gaseous hydrogen peroxide in the air with the absorbent, and then be absorbed into the absorbent solution, the time it takes for air to pass through the water bath temperature-controlled collecting spiral tube 11 is still relatively short compared to the tiny solid particulate hydrogen peroxide in the air. This is insufficient to dissolve and absorb the solid particulate hydrogen peroxide, and the smaller the particle size, the lower the possibility of absorption. Hydrogen peroxide ultrafine particles with a particle size of less than 2.5 μm, especially less than 0.1 μm, pose the greatest harm to the human body when they enter the bloodstream. Therefore, effective measurement of the content of solid particulate hydrogen peroxide in the air is of great significance for environmental air monitoring. Thus, it is necessary to further configure a solid absorption component 20 to fully absorb the solid particulate hydrogen peroxide.

[0071] In one optional embodiment of this application, the solid-state absorption component 20 may include:

[0072] The first gas-liquid separation chamber 21 and the steam trap 22 are connected; the first gas-liquid separation chamber 21 is connected to the output end of the water bath temperature-controlled trapping spiral tube 11 through the first three-way valve 71; the steam trap 22 is connected to the input end of the first fluorescence measurement channel 40 and the input end of the second fluorescence measurement channel 50 through the second three-way valve 72.

[0073] It is understandable that the output end of the second three-way valve 72 and the input end of the first fluorescence measurement channel 40 and the input end of the second fluorescence measurement channel 50 can be directly connected or indirectly connected.

[0074] Optionally, the output of the second three-way valve 72 can also be connected to the input of the first fluorescence measurement channel 40 and the input of the second fluorescence measurement channel 50 through the second gas-liquid separation chamber 9, respectively.

[0075] Therefore, the connection structure of the solid-state absorption component 20 can specifically include the output end of the water bath temperature-controlled trapping spiral tube 11 being connected to the input end of the first three-way valve 71; the first output end of the first three-way valve 71 being connected to the input end of the first gas-liquid separation chamber 21; the gas output end of the first gas-liquid separation chamber 21 being connected to the input end of the steam trap 22; the second output end of the first three-way valve 71, the liquid output end of the first gas-liquid separation chamber 21, and the output end of the steam trap 22 being connected to the first input end of the second three-way valve 72; the output end of the liquid absorption component being connected to the second input end of the second three-way valve 72; the output end of the second three-way valve 72 being connected to the input end of the second gas-liquid separation chamber 9; and the liquid output end of the second gas-liquid separation chamber 9 being connected to the input ends of the first fluorescence measurement channel 40 and the second fluorescence measurement channel 50.

[0076] like Figure 1As shown, in this embodiment, the vapor trap 22 is the main component of the solid absorption assembly 20 that absorbs solid hydrogen peroxide particles. The liquid output from the water bath temperature-controlled trapping spiral tube 11 is a gas-liquid mixture containing the absorbed gaseous hydrogen peroxide and the remaining air. The solid particulate hydrogen peroxide is in the air of the gas-liquid mixture. Therefore, in this embodiment, a first gas-liquid separation chamber 21 is further configured for the vapor trap 22. The gas-liquid mixture is separated by the first gas-liquid separation chamber 21, so that the gaseous part is separated and introduced into the vapor trap 22. The atomized steam at a temperature of up to 200°C absorbs and dissolves the particulate matter in the gas, so that the particulate matter can quickly absorb a large amount of water vapor and gradually dissolve. Then, the mixture is cooled and condensed by the low-temperature mixed gas flow at about 10°C. The condensed water droplets are collected at the bottom, and a solution containing dissolved and absorbed hydrogen peroxide is obtained. This solution can be mixed with the liquid separated from the first gas-liquid separation chamber 21 and used as the test liquid for subsequent detection. Of course, what is discharged from the output end of the steam trap 22 is not only liquid, but also a small amount of gas, which can be discharged as waste gas together with the gas output end of the subsequent second gas-liquid separation chamber 9.

[0077] like Figure 3 , Figure 4 and Figure 5 As shown, Figure 3 and Figure 4 All data are particulate matter content in the range of 10 nm to 1000 nm, obtained by scanning electromobility particle size analyzer. Figure 3 and Figure 4 The x-axis represents the particle size, and the y-axis represents the particle number concentration. Figure 3 The image shows the data for the content of each particulate matter in the air collected at two intervals of 5 seconds. Figure 4 yes Figure 3 The data corresponds to the particulate matter content remaining after the air from the two samples passed through the steam trap 22. However, the particulate matter content of one of the air samples was so low that it was considered completely absorbed and therefore not included in the data. Figure 4 It is displayed in the middle.

[0078] in, Figure 3 Data on the content of particulate matter of different sizes contained in the gas portion after the air passes through the water bath temperature-controlled trapping spiral tube 11; Figure 4 This refers to the particulate matter content in the exhaust gas after the air passes through the water bath temperature-controlled trapping spiral tube 11 and the particulate matter is fully dissolved and absorbed by the steam trap 22; Figure 3 and Figure 4A comparison clearly shows that the water bath temperature-controlled trapping spiral tube 11 has insufficient capacity to dissolve and absorb solid particulate matter. Furthermore, it is understandable that not all particulate matter in the air is hydrogen peroxide; it may also be particulate matter from other common airborne substances, or particulate matter formed by the adsorption of hydrogen peroxide and other substances. After passing through the vapor trap 22, all of these can be dissolved and absorbed. The test liquid output from the vapor trap 22, which contains dissolved hydrogen peroxide and other substances, will not interfere with the measurement results even if the test liquid contains substances other than hydrogen peroxide, because the subsequent first fluorescence measurement channel 40 and second fluorescence measurement channel 50 are only equipped with enzymes capable of reacting with peroxide to generate fluorescent substrates.

[0079] like Figure 5 As shown, Figure 5 The image shows a comparison of the changes in hydrogen peroxide concentration in the air measured with and without the absorption of hydrogen peroxide from particulate matter. According to... Figure 5 As shown, it is clear that hydrogen peroxide is indeed present in the dissolved and absorbed particulate matter in the air.

[0080] Combination Figures 3 to 5 It is known that when monitoring the hydrogen peroxide content in the atmosphere, if only the water bath temperature-controlled trapping spiral tube 11 is used to capture and absorb hydrogen peroxide in the air, only gaseous hydrogen peroxide can be absorbed. The absorption of solid particulate hydrogen peroxide is minimal or almost non-existent, and thus solid hydrogen peroxide particles will not enter the subsequent fluorescence detection process. This means that the final fluorescence detection result only characterizes the content of gaseous hydrogen peroxide in the air, leading to a significant underestimation of the measured hydrogen peroxide content. Therefore, this application simultaneously includes a gaseous absorption component 10 and a solid absorption component 20, enabling more comprehensive and reliable absorption of both gaseous and solid particulate hydrogen peroxide in the atmosphere, thereby improving the accuracy and reliability of monitoring the hydrogen peroxide content in the atmosphere.

[0081] like Figure 6 As shown, Figure 6 The horizontal axis represents the particle size of the particulate matter, and the vertical axis represents the absorption rate. Figure 6 In this context, T represents the temperature of the high-temperature steam, and pump refers to the rotational speed of the peristaltic pump that provides the water needed to generate the high-temperature steam for the steam trap. Figure 6 The absorption rates of particles of different sizes in the steam trap 22 using high-temperature steam at different temperatures are shown. Clearly, when the high-temperature steam is at 200°C, the steam trap 22 exhibits a high dissolution and absorption rate for particles of most sizes.

[0082] Based on this, in order to meet different measurement purposes for hydrogen peroxide content, in this embodiment, a first three-way valve 71 is provided between the output end of the water bath temperature-controlled trapping spiral tube 11 and the first gas-liquid separation chamber 21, and a second three-way valve 72 is also provided between the second gas-liquid separation chamber 9 and the steam trapping trap 22. Therefore, if it is necessary to measure the absorption of hydrogen peroxide in solid particles in the gas-liquid mixture after the gas-liquid mixture output from the water bath temperature-controlled trapping spiral tube 11 is input into the first three-way valve 71, the first output end of the first three-way valve 71 can be opened and the second output end closed, so that the gas-liquid mixture is output from the first output end of the first three-way valve 71 to the first gas-liquid separation chamber 21. After gas-liquid separation in the first gas-liquid separation chamber 21, the gas part enters the steam trap 22 and the liquid part flows to the first input end of the second three-way valve 72. After the steam trap 22 dissolves the hydrogen peroxide in the solid particles in the gas using high-temperature steam, it also forms a test liquid that has absorbed hydrogen peroxide and outputs it to the first input end of the second three-way valve 72.

[0083] If it is not necessary to perform hydrogen peroxide absorption detection of solid particles on the gas-liquid mixture output from the water bath temperature-controlled trapping spiral tube 11 (for example, if it is clear that there is no hydrogen peroxide with solid particles in the sample gas input from the gas collection port), then the first output end of the first three-way valve 71 can be closed and the second output end opened, so that the gas-liquid mixture is directly input from the second output end of the first three-way valve 71 to the first input end of the second three-way valve 72.

[0084] Optionally, a three-way valve can be installed at the liquid output end of the first gas-liquid separation chamber 21. The input end of the three-way valve is connected to the liquid output end of the first gas-liquid separation chamber 21, and the first output end is connected to the waste liquid recovery device. The second output end is connected to the first input end of the second three-way valve 72. When it is only necessary to measure the hydrogen peroxide content of solid particles in the sample gas, the first output end of the three-way valve can be controlled to open and the second output end to close, so that the liquid output from the liquid output end of the first gas-liquid separation chamber 21 is discharged as waste liquid and not input to the first input end of the second three-way valve 72.

[0085] Based on this, the liquid or gas-liquid mixture input from the first input terminal of the second three-way valve 72 flows from the output terminal of the second three-way valve 72 to the second gas-liquid separation chamber 9, thus entering the subsequent gas-liquid separation process. Additionally, the second input terminal of the second three-way valve 72 is also connected to the output terminal of the liquid absorption assembly 30; therefore, in practical applications, the measurement of hydrogen peroxide can be performed on either the sample liquid collected by the liquid absorption assembly 30 or the sample gas collected at the gas collection port, depending on the actual measurement needs.

[0086] The gas, liquid, or gas-liquid mixture output from the output end of the second three-way valve 72 is input into the second gas-liquid separation chamber 9 for further gas-liquid separation. The gas portion can be discharged as waste gas through the filter membrane 91 and the air pump 92 in sequence. The liquid discharged from the second gas-liquid separation chamber 9 is the main liquid to be tested for subsequent hydrogen peroxide measurement.

[0087] Based on the above discussion, this embodiment utilizes the control and regulation of three-way valves at different positions to achieve the collection of hydrogen peroxide analytes in various states, thereby flexibly meeting the measurement needs of hydrogen peroxide in various application scenarios and facilitating the widespread application of hydrogen peroxide content measurement equipment.

[0088] As described above, in this embodiment, both the first fluorescence measurement channel 40 and the second fluorescence measurement channel 50 can achieve fluorescence detection of peroxides. However, the difference is that in the second fluorescence measurement channel 50, the input test liquid is first removed with hydrogen peroxide before fluorescence detection, while the first fluorescence measurement channel 40 directly performs fluorescence detection on the input test liquid. It can be seen that for the same test liquid, the difference in the results of detection by the two fluorescence measurement channels is caused by the presence or absence of hydrogen peroxide. Therefore, based on the comparison of the data measured by the two sets of fluorescence measurement channels, a more accurate and reliable measurement result can be obtained.

[0089] The first fluorescence measurement channel 40 and the second fluorescence measurement channel 50 will be described in detail below with specific embodiments.

[0090] In one optional embodiment of this application, the first fluorescence measurement channel 40 may include a first spiral tube 41, a first water bath temperature control spiral tube 42, a first pH adjustment spiral tube 43, and a first fluorescence detection chamber 44 connected in sequence.

[0091] The second fluorescence measurement channel 50 may include a second spiral tube 51, a second water bath temperature control spiral tube 52, a second pH adjustment spiral tube 53, and a second fluorescence detection chamber 54 connected in sequence.

[0092] The input ends of the first helical tube 41 and the second helical tube 51 are used to receive the first test solution and the second test solution, respectively; and the input end of the first helical tube 41 is connected to the first liquid container 84 through the peristaltic pump 100; the first liquid container 84 is used to store potassium hydrogen phthalate buffer solution; the input end of the second helical tube 51 is connected to the second liquid container 85 through the peristaltic pump 100; the second liquid container 85 is used to store potassium hydrogen phthalate buffer solution dissolved with hydrogen peroxide catalytic enzyme.

[0093] The input ends of the first water bath temperature-controlled spiral tube 42 and the second water bath temperature-controlled spiral tube 52 are respectively connected to a third liquid container 86 through a peristaltic pump 100. The third liquid container 86 is used to store a mixture of puerarin and peroxidase.

[0094] The input ends of the first pH adjusting spiral tube 43 and the second pH adjusting spiral tube 53 are respectively connected to the pH adjusting liquid container 87 through the peristaltic pump 100;

[0095] The data processing module 60 is connected to the first fluorescence detection chamber 44 and the second fluorescence detection chamber 54 respectively, and is used to compare the first fluorescence detection data measured by the first fluorescence detection chamber 44 and the second fluorescence detection data measured by the second fluorescence detection chamber 54 to obtain hydrogen peroxide content data.

[0096] like Figure 1 As shown, the test liquid output from the liquid output terminal of the second gas-liquid separation chamber 9 is divided into two parts: the test liquid input into the first fluorescence measurement channel 40 is the first test liquid, and the test liquid input into the second fluorescence measurement channel 50 is the second test liquid. The amount of the first test liquid can be exactly the same or different. If they are different, as long as the ratio between the amount of the first test liquid and the amount of the second test liquid is clearly determined, the detection data of the two sets of test liquids can be used as a reference for each other. This will not be elaborated in detail in this application. In this embodiment, it is assumed that the first test liquid and the second test liquid are obtained by equally dividing the same test liquid, which will not be repeated hereafter.

[0097] Based on this, the first and second test solutions are respectively input into the first fluorescence measurement channel 40 and the second fluorescence measurement channel 50, and then first enter the first spiral tube 41 and the second spiral tube 51, respectively. The first spiral tube 41 is connected to the first liquid container 84 through a peristaltic pump 100, and the second spiral tube 51 is connected to the second liquid container 85 through a peristaltic pump 100. Therefore, the first spiral tube 41 serves as a container for the full contact and reaction of the first test solution and the solution output from the first liquid container 84, while the second spiral tube 51 serves as a container for the full contact and reaction of the second test solution and the solution output from the second liquid container 85.

[0098] Based on this, the solutions stored in the first liquid container 84 and the second liquid container 85 in this embodiment are not the same; the solution stored in the second liquid container 85 is a mixture of potassium hydrogen phthalate and hydrogen peroxide catalytic enzyme.

[0099] The solution stored in the second liquid container 85 can be diluted with a syringe by taking 1 ml of hydrogen peroxide catalytic enzyme (refrigerated and shaken well before use; if the original solution is yellow-green, it indicates that it is not completely mixed; the original solution should be a dark, viscous particulate suspension) and diluting it to 100 ml with potassium hydrogen phthalate buffer to form a hydrogen peroxide catalytic enzyme stock solution; then, take about 20 ml of the hydrogen peroxide catalytic enzyme stock solution and add it to 2 L of potassium hydrogen phthalate buffer to obtain a mixture of potassium hydrogen phthalate and hydrogen peroxide catalytic enzyme.

[0100] The mixture is introduced into the second spiral tube 51 mainly to utilize the hydrogen peroxide catalytic enzyme in it to fully contact and react with the hydrogen peroxide in the second test solution, thereby removing the hydrogen peroxide in the second test solution, that is, to obtain a second test solution with hydrogen peroxide removed, which can be used as a reference group data for the first test solution.

[0101] Unlike the solution stored in the second liquid container 85, the potassium hydrogen phthalate buffer solution stored in the first liquid container 84 does not chemically react with the first test solution; it is merely a process to ensure that the first and second test solutions have the same mixed potassium hydrogen phthalate buffer solution.

[0102] Therefore, it can be seen that there is a difference between the first test solution and the second test solution output from the first spiral tube 41 and the second spiral tube 51, respectively, whether or not hydrogen peroxide has been removed. This allows the second test solution to be used as a reference group for the first test solution, so as to eliminate the interference caused by other peroxides in the test solution in the subsequent fluorescence reaction.

[0103] The first and second test solutions, output from the first spiral tube 41 and the second spiral tube 51 respectively, then enter the first and second water bath temperature-controlled spiral tubes 42 and 52 respectively. The input ends of both the first and second water bath temperature-controlled spiral tubes 42 and 52 are connected to a third liquid container 86 storing a mixture of puerarin and peroxidase. Thus, within the first and second water bath temperature-controlled spiral tubes 42 and 52, the first and second test solutions can be thoroughly mixed with the mixture of puerarin and peroxidase. Since the peroxidases used are mostly plant and animal extracts, a suitable temperature is required for efficient reaction. Water with a high specific heat capacity can be used as the heat transfer medium in both the first and second water bath temperature-controlled spiral tubes 42 and 52. Constant-temperature circulating water is used to fill the outer layer of the spiral tubes with circulating water at a set temperature, which can be between 30℃ and 37℃, maintaining a stable and uniform reaction temperature for each test.

[0104] It should be noted that the principle of fluorescence detection of hydrogen peroxide is based on the liquid-phase reaction of peroxide and fluorescent substrate under the catalysis of peroxidase, generating a fluorescent substance, which is a substance that can be excited by laser irradiation and output fluorescence. The content of this fluorescent substance is directly proportional to the content of peroxide, and the fluorescence intensity output by the excited fluorescent substance is also directly proportional to the content of the fluorescent substance. Therefore, the content of peroxide can be determined based on the measured fluorescence signal intensity. In this embodiment, puerarin, which has a high fluorescence intensity, is used as the fluorescent substrate. The peroxides in the first and second test solutions undergo liquid-phase reactions with puerarin under the catalysis of peroxidase, and the resulting fluorescent dimers can be excited at 315 nm and output fluorescence light in the range of 460 nm to 485 nm.

[0105] In addition, horseradish peroxidase can be used as the peroxidase in this embodiment. In practical applications, 500 mg of puerarin and 30 mg of peroxidase (e.g., Peroxidase, Sigma 8375, which needs to be refrigerated; if using Sigma 8250 peroxidase, then 120 mg needs to be weighed) can be added to 2 L of potassium hydrogen phthalate buffer to dissolve and obtain a mixture of puerarin and peroxidase.

[0106] Based on this, the first and second test solutions, under the temperature conditions of 30℃-37℃ provided by the first water bath temperature-controlled spiral tube 42 and the second water bath temperature-controlled spiral tube 52, respectively, react with puerarin under the action of horseradish peroxidase to generate o-hydroxyphenylacetic acid. Obviously, the first test solution contains all peroxides, including hydrogen peroxide, that react to generate o-hydroxyphenylacetic acid, while the second test solution should only contain the o-hydroxyphenylacetic acid generated by the reaction of the remaining peroxides after the removal of hydrogen peroxide.

[0107] Based on this, the reaction product solution output from the first water bath temperature control spiral tube 42 and the second water bath spiral tube is further input into the first pH adjustment spiral tube 43 and the second pH adjustment spiral tube 53, respectively, and the pH value is adjusted by using the pH adjustment liquid provided by the pH adjustment liquid container 87. The pH adjustment liquid can be NaOH solution or other pH adjustment liquid, and the pH of the reaction product solution is adjusted to 9-10.

[0108] After pH adjustment, the first fluorescence detection chamber 44 and the second fluorescence detection chamber 54 can be used to perform fluorescence detection on the two reaction product solutions obtained respectively. The measured data are the detection data that can characterize the peroxide content in the first test solution and the second test solution after removing hydrogen peroxide. The data processing module 60 performs difference calculation on the two sets of detection data to obtain the result characterizing the peroxide content.

[0109] Based on the above discussion, in this embodiment, the first fluorescence measurement channel 40 and the second fluorescence measurement channel 50 are each equipped with three spiral tubes to enable the test liquid to be thoroughly mixed or reacted with different solutions in sequence. On this basis, when the peroxide and puerarin in the test liquid undergo a chemical reaction, a stable and uniform temperature environment is provided by the first water bath temperature-controlled spiral tube 42 and the second water bath temperature-controlled spiral tube 52, respectively, to ensure that the reaction process is fully and effectively carried out, thereby ensuring the accuracy and reliability of subsequent fluorescence detection.

[0110] Based on this, in another optional implementation of this embodiment, the first liquid container 84, the second liquid container 85, the third liquid container 86, and the pH adjustment solution container 87 are all brown nozzle bags; this avoids cross-contamination of the solution containers during long-term use, and also improves the problem of solution contamination caused by the entry of outside air during use, reducing the use and waste of expensive enzyme reagents. Furthermore, a peristaltic pump 100 is installed between each solution container and the connected spiral tube, making the solution flow more smoothly.

[0111] Further, alternatively, in another implementation of this embodiment, such as Figure 1 As shown, air venting tees 200 can be installed between the first spiral tube 41 and the first water bath temperature-controlled spiral tube 42, between the first water bath temperature-controlled spiral tube 42 and the first pH adjustment spiral tube 43, between the first pH adjustment spiral tube 43 and the first fluorescence detection chamber 44, between the second spiral tube 51 and the second water bath temperature-controlled spiral tube 52, between the second water bath temperature-controlled spiral tube 52 and the second pH adjustment spiral tube 53, and between the second pH adjustment spiral tube 53 and the second fluorescence detection chamber 54. These tees can fully release the air bubbles mixed in the liquid output from the three spiral tubes, thus avoiding instability in the entire liquid circuit due to air bubble interference.

[0112] Based on any of the above embodiments, in the process of determining the hydrogen peroxide content data according to the first fluorescence detection data and the second fluorescence detection data measured by the first fluorescence detection chamber 44 and the second fluorescence detection chamber 54 respectively, the data processing module 60 also needs to pre-calibrate the response relationship between the concentration of hydrogen peroxide and the fluorescence detection data in the hydrogen peroxide content measuring device.

[0113] Based on this, in an optional embodiment of this application, the hydrogen peroxide content measuring device may further include:

[0114] Standard liquid container 82, cleaning liquid container 83, third three-way valve 73 and fourth three-way valve 74;

[0115] The output end of the absorption liquid container 81 and the output end of the standard liquid container 82 are respectively connected to the first input end and the second input end of the third three-way valve 73; the output end of the third three-way valve 73 is connected to the first input end of the peristaltic pump 100 and the fourth three-way valve 74.

[0116] The output end of the cleaning fluid container 83 is connected to the second input end of the fourth three-way valve 74; the output end of the fourth three-way valve 74 is connected to the first input end of the water bath temperature control and trapping spiral tube 11.

[0117] It should be noted that the standard solution stored in the standard solution container 82 in this embodiment is a hydrogen peroxide solution of a specific concentration specially prepared with hydrogen peroxide; and the standard solution container should be detachable and replaceable in actual applications so that different concentrations of hydrogen peroxide solution can be replaced during the calibration process. After different concentrations of hydrogen peroxide solution pass through the hydrogen peroxide content measuring device in sequence, the fluorescence response data obtained by fluorescence detection can be obtained. Based on the fluorescence response data corresponding to different concentrations of hydrogen peroxide, the corresponding relationship between the two can be fitted, thereby realizing the calibration of the response relationship between the concentration of hydrogen peroxide and the fluorescence detection data.

[0118] Furthermore, in the actual calibration process between the concentration of hydrogen peroxide and the corresponding relationship of fluorescence detection data, the first input terminal of the fourth three-way valve 74 can be closed first, and the second input terminal of the fourth three-channel can be opened. This allows the cleaning solution stored in the cleaning solution container 83 to flow sequentially through the water bath temperature-controlled trapping spiral tube 11 and its subsequent fluid channels, ultimately flowing through the first fluorescence measurement channel 40 and the second fluorescence measurement channel 50 respectively, to clean residual substances in the entire fluid channel. Specifically, the cleaning solution can be a hydrochloric acid solution. Additionally, in this embodiment, the standard solution and cleaning solution can also be in brown nozzle bags.

[0119] In the above embodiments, a liquid cleaning solution is used to clean the entire fluid channel. In practical applications, it is also necessary to purge the entire fluid channel of residual gas. Therefore, in an optional embodiment of this application, the hydrogen peroxide content measuring device may further include:

[0120] A fifth three-way valve 75 and a peroxide removal device 12 are also provided between the water bath temperature-controlled trapping spiral tube 11 and the gas collection port;

[0121] The gas collection port is connected to the first input end of the peristaltic pump 100 and the fifth three-way valve 75; the first output end of the fifth three-way valve 75 is connected to the second input end of the water bath temperature-controlled trapping spiral tube 11; the second output end of the fifth three-way valve 75 is connected to the input end of the peroxide removal device 12; and the output end of the peroxide removal device 12 is connected to the second input end of the water bath temperature-controlled trapping spiral tube 11.

[0122] In this embodiment, the gas sampling port is used as the input port for nitrogen or other gases. The first output end of the fifth three-way valve 75 is closed and the second output end is opened, so that nitrogen and other gases pass through the peroxide removal device 12. This ensures that the peroxides in the nitrogen and other gases are fully absorbed, resulting in zero-peroxide gas (i.e., gas without peroxides). This gas is then used as the cleaning gas to clean the fluid channels inside the entire device, further ensuring the cleanliness of the fluid channels inside the device.

[0123] Based on the above discussion, the hydrogen peroxide content measuring device in this application can detect the content of peroxides in different forms, such as gaseous, particulate, and liquid, online. The entire reaction is divided into three parts: sample collection and two measurement channels. Each part is relatively independent and has the function of venting air bubbles in the measurement channel. This prevents air bubbles from affecting the driving efficiency of the peristaltic pump 100 and causing instability in the entire fluid channel, ensuring that the entire device can operate stably for a long time, providing accurate data and reducing pipeline interference.

[0124] In addition, the hydrogen peroxide content measuring device described in this application can also be used to detect some other peroxides. For example, it can be used to measure soluble organic peroxides, such as peroxyphenylacetyl.

[0125] In summary, this application configures a gaseous absorption component, a solid absorption component, and a liquid absorption component in the detection device for detecting hydrogen peroxide content. The solid absorption component mainly includes a first gas-liquid separation chamber and a vapor trap. The first gas-liquid separation chamber separates the liquid to be tested and the gaseous part containing particulate matter from the gas-liquid mixture output from the gaseous absorption component. The vapor trap further dissolves and absorbs the particulate matter containing hydrogen peroxide. Thus, by the cooperation of the gaseous absorption component, the solid absorption component, and the liquid absorption component, it is possible not only to collect gaseous hydrogen peroxide in the air, but also to absorb and collect particulate solid hydrogen peroxide, and to collect and measure hydrogen peroxide dissolved in liquids such as rainwater through the liquid absorption component. This application collects hydrogen peroxide from multiple different dimensions, thereby more comprehensively and accurately measuring different forms of hydrogen peroxide in the ambient atmosphere and improving the accuracy and reliability of hydrogen peroxide content measurement. Furthermore, during the hydrogen peroxide content measurement process, two fluorescence measurement channels are utilized. Two test liquids containing hydrogen peroxide from the same source are divided into two portions. One portion of the test liquid is directly subjected to fluorescence detection in the first fluorescence measurement channel, obtaining the first fluorescence detection data. The other portion of the test liquid is first subjected to fluorescence detection in the second fluorescence measurement channel to remove the hydrogen peroxide, obtaining the second fluorescence detection data as a reference. Based on the comparison between the first and second fluorescence detection data, interference from other peroxides besides hydrogen peroxide on the measurement results can be effectively eliminated, further improving the accuracy and reliability of hydrogen peroxide content measurement.

[0126] Based on the above discussion, this application also provides a method for measuring the content of hydrogen peroxide, which is applied to the hydrogen peroxide content measuring device as described in any of the preceding claims.

[0127] like Figure 2 As shown, in one specific embodiment of this application, the method for measuring the hydrogen peroxide content may include:

[0128] S11: Absorb gaseous hydrogen peroxide in the sample gas to be tested through the gaseous absorption component and / or absorb hydrogen peroxide particles in the sample gas to be tested through the solid absorption component and / or collect dissolved hydrogen peroxide in the sample liquid to be tested through the liquid absorption component to obtain the sample liquid to be tested.

[0129] S12: Divide the liquid to be tested into a first test liquid and a second test liquid;

[0130] S13: Perform fluorescence detection on the first test liquid through the first fluorescence measurement channel to obtain the first fluorescence detection data;

[0131] S14: Remove hydrogen peroxide from the second test solution through the second fluorescence measurement channel, and perform fluorescence detection on the second test solution after removing hydrogen peroxide to obtain second fluorescence detection data;

[0132] S15: Compare and calculate the first fluorescence detection data and the second fluorescence detection data to obtain the hydrogen peroxide content.

[0133] In this embodiment, based on actual measurement needs, peroxides in different forms such as gaseous, particulate, and liquid states can be collected and detected using gaseous absorption component 10, solid absorption component 20, and liquid absorption component. Two fluorescence measurement channels are used for cross-reference and comparison, and hydrogen peroxide in one of the measurement channels is removed to serve as a basis for eliminating interference from other peroxides. Finally, the difference calculation is performed based on the detection data obtained from the two measurement channels to obtain more accurate and reliable measurement results.

[0134] Based on the above discussion, in practical applications, the concentration response curve between the hydrogen peroxide concentration in the analyte and the corresponding fluorescence detection data can also be pre-calibrated.

[0135] In an optional embodiment of this application, the process of calibrating the concentration response curve may include:

[0136] S101: Prepare standard solutions containing different concentrations of hydrogen peroxide;

[0137] S102: Divide each concentration of hydrogen peroxide standard solution into two portions and pass them into the first fluorescence measurement channel and the second fluorescence measurement channel respectively to obtain the first calibration detection data and the second calibration detection data corresponding to each different concentration of hydrogen peroxide standard solution.

[0138] S103: Perform a difference calculation on the first and second calibration test data corresponding to the same concentration of hydrogen peroxide standard solution to obtain the calibration test data of hydrogen peroxide standard solution;

[0139] S104: Based on the calibration and detection data corresponding to hydrogen peroxide standard solutions of different concentrations and the hydrogen peroxide concentration, a linear fit is performed to obtain the concentration response curve.

[0140] The standard solution preparation process in this embodiment can be as follows:

[0141] 1) Take 1 mL of 3wt% hydrogen peroxide solution and dilute it with cold absorption buffer (i.e., potassium hydrogen phthalate buffer) to make up to 100 mL, and prepare solution ① (theoretical concentration is 300 mg / L).

[0142] 2) Take 1 mL of solution ①, dilute it with cold absorption solution to a final volume of 100 mL, and prepare solution ② (theoretical concentration is 3 mg / L).

[0143] 3) Take 1 mL of solution ②, dilute it with cold absorption solution to a final volume of 100 mL, and prepare a 30 ug / L standard solution;

[0144] 4) Take 500 μL of solution ②, dilute it with cold absorption solution to a final volume of 100 mL, and prepare a 15 μg / L standard solution;

[0145] 5) Take 200 μL of solution ②, dilute it with cold absorption solution to a final volume of 100 mL, and prepare a 6 μg / L standard solution;

[0146] 6) Take 100 μL of solution ②, dilute it with cold absorption solution to a final volume of 100 mL, and prepare a 3 μg / L standard solution;

[0147] 7) Take 50 μL of solution ②, dilute it with cold absorption solution to a final volume of 100 mL, and prepare a 1.5 μg / L standard solution.

[0148] Combination Figure 1 Closing the first input of the third three-way valve 73 and opening the second input will cause the standard solution container 82 to deliver hydrogen peroxide standard solution of any concentration (e.g., 30 ug / L) from 30 ug / L, 15 ug / L, 6 ug / L, 3 ug / L, and 1.5 ug / L to the water bath temperature-controlled trapping spiral tube 11 via the peristaltic pump 100. The solution will then be divided into two portions flowing into the first fluorescence measurement channel 40 and the second fluorescence measurement channel 50, obtaining one set of fluorescence detection data without hydrogen peroxide removal and one set of fluorescence detection data with hydrogen peroxide removal. The difference between the two is the response data. Next, closing the first input of the fourth three-way valve 74 and opening the second input of the fourth three-way channel, and cleaning the fluid channels in the entire device, will replace the standard solution container 82 with one containing a different concentration of hydrogen peroxide standard solution (e.g., 15 ug / L), and obtaining a corresponding response data in the same manner as described above. Similarly, by following a similar method, the corresponding response data obtained by fluorescence detection at multiple different hydrogen peroxide concentrations can be obtained.

[0149] The concentration of liquid peroxide is calculated according to Lambert-Beer's law. ;in, The fluorescence signal intensity, The intensity of the zero-point optical signal. For coefficients, The concentration of peroxide in the test solution; obtained according to the zero-calibration process. The concentration of the peroxide standard solution obtained during the calibration process and its fluorescence signal intensity The coefficients are obtained by calculating the calibration curve. ; using the obtained coefficients Peroxide concentration in the liquid phase It can be done Converted to the content of gaseous peroxides in the sample gas. This represents the concentration of gaseous peroxides (in μg / L). Represents the concentration of liquid phase peroxides (unit: μg / L). The liquid flow rate (in L / min) in the water bath temperature-controlled trapping spiral tube 11 is given. The gas flow rate (in L / min) in the water bath temperature-controlled trapping spiral tube 11 is given. The water bath temperature-controlled trapping spiral tube 11 has a trapping efficiency of up to 100% for gaseous peroxides in the sample gas.

[0150] In addition, the above-mentioned zeroing process can use high-purity nitrogen gas, zero sample gas, or other similar sample gas as the sample gas to be tested. During the process of measuring the concentration of peroxide, the first fluorescence measurement channel 40 and the second fluorescence measurement channel 50 respectively detect the fluorescence detection data corresponding to the zero point peroxide. Theoretically, they should all be 0, but in actual detection, they may all be very low values, which are reflected as the intercepts of the coordinate axes in the calibration curve.

[0151] Based on the above discussion, and referring to... Figure 1 and Figure 7 In another optional embodiment of this application, the process of a hydrogen peroxide content measuring device detecting peroxides in the air may include:

[0152] 1) Under the action of the peristaltic pump 100, air and absorbent enter the low temperature (10℃) water bath temperature-controlled trapping spiral tube 11. The absorbent and air are fully contacted and absorbed. Peroxides in the air (including organic peroxides and peroxides) are captured and absorbed by the 10℃ absorbent and output from the water bath temperature-controlled trapping spiral tube 11.

[0153] 2) The test solution output from the water bath temperature-controlled trapping spiral tube 11 undergoes gas-liquid separation in the second gas-liquid separation chamber 9 (without passing through the steam trap 22). Air is discharged from above through the filter membrane 91, flow meter and air pump 92. Excess liquid can be discharged by the peristaltic pump 100.

[0154] 3) The test solution output from the second gas-liquid separation chamber 9 is divided into two parts and enters the two spiral tubes of channel A (first fluorescence measurement channel 40) and channel B (second fluorescence measurement channel 50) respectively. They react fully with solution A (i.e., potassium hydrogen phthalate buffer) and solution B (potassium hydrogen phthalate buffer containing hydrogen peroxide catalytic enzyme). The hydrogen peroxide decomposing enzyme in solution B decomposes the hydrogen peroxide in the captured test solution, leaving only other peroxides. Solution A does not contain any added peroxidase, so the hydrogen peroxide remains in the solution.

[0155] 4) In channels A and B, the liquids after being mixed and reacted with solutions A and B are respectively introduced into reaction spiral tubes with a temperature controlled by a 30°C water bath, so that peroxides and puerarin are converted into o-hydroxyphenylacetic acid under the action of horseradish peroxidase.

[0156] 5) Add NaOH or other pH-adjusting solutions to the o-hydroxyphenylacetic acid solution to adjust the pH to 10 or other suitable values; ensure that there are no air bubbles in the liquid by using a defoaming tee, and then let the two o-hydroxyphenylacetic acid solutions enter the corresponding fluorescence detection chambers respectively;

[0157] 6) The concentration of all peroxides in the air is calculated from the fluorescence signal intensity measured by channel A, while the concentration of other peroxides in the air except for hydrogen peroxide is calculated from the fluorescence signal intensity measured by channel B.

[0158] 7) Perform a difference calculation on the concentrations of peroxides obtained from channels A and B respectively to obtain the concentration of hydrogen peroxide in the air.

[0159] It should be noted that, in this document, relational terms such as "first" and "second" are used merely to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that the elements inherent in a process, method, article, or apparatus that includes a list of elements are included. Without further limitations, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes said element. Additionally, portions of the technical solutions provided in the embodiments of this application that are consistent with the implementation principles of corresponding technical solutions in the prior art have not been described in detail to avoid excessive elaboration.

[0160] This article uses specific examples to illustrate the principles and implementation methods of the present invention. The descriptions of the above embodiments are only for the purpose of helping to understand the method and core ideas of the present invention. It should be noted that those skilled in the art can make several improvements and modifications to the present invention without departing from the principles of the present invention, and these improvements and modifications also fall within the protection scope of the present invention.

Claims

1. A device for measuring the content of hydrogen peroxide, characterized in that, include: The liquid absorption assembly is used to collect dissolved hydrogen peroxide in the sample solution to obtain the test liquid; A gaseous absorption component is used to absorb gaseous hydrogen peroxide from the sample gas to obtain the liquid to be tested. A solid-state absorption assembly is used to collect dissolved hydrogen peroxide in a sample solution to obtain a test liquid. The solid-state absorption assembly includes a first gas-liquid separation chamber and a vapor trap, and is connected to a first three-way valve and a second three-way valve. The output end of the gas-state absorption assembly is connected to the input end of the first three-way valve. The first output end of the first three-way valve is connected to the input end of the first gas-liquid separation chamber. The gas output end of the first gas-liquid separation chamber is connected to the input end of the vapor trap. The second output end of the first three-way valve, the liquid output end of the first gas-liquid separation chamber, and the output end of the vapor trap are all connected to the first input end of the second three-way valve. The output end of the liquid absorption assembly is connected to the second input end of the second three-way valve. The output end of the second three-way valve is connected to the input ends of both the first and second fluorescence measurement channels, so as to separate the test liquid output from the second three-way valve into a first test liquid and a second test liquid. The first fluorescence measurement channel is used to detect and obtain the first fluorescence detection data of the first test solution; The second fluorescence measurement channel is used to detect and obtain the second fluorescence detection data of the second test solution after removing hydrogen peroxide; The data processing module is used to compare and process the first fluorescence detection data and the second fluorescence detection data to obtain hydrogen peroxide content data.

2. The hydrogen peroxide content measuring device as described in claim 1, characterized in that, The gaseous absorption assembly includes a water bath temperature-controlled trapping spiral tube and an absorption liquid container connected to the first input end of the water bath temperature-controlled trapping spiral tube via a peristaltic pump; the absorption liquid container is used to store an absorption liquid whose main component is potassium hydrogen phthalate buffer solution. The second input end of the water bath temperature-controlled trapping spiral tube is connected to the gas collection port; The input end of the first three-way valve is connected to the output end of the water bath temperature control and trapping spiral tube.

3. The hydrogen peroxide content measuring device as described in claim 2, characterized in that, The output end of the second three-way valve is connected to the second gas-liquid separation chamber; the liquid output end of the second gas-liquid separation chamber is connected to both the first fluorescence measurement channel and the input end of the second fluorescence measurement channel.

4. The hydrogen peroxide content measuring device as described in claim 2, characterized in that, It also includes a standard liquid container, a cleaning fluid container, a third three-way valve, and a fourth three-way valve; The output end of the absorption liquid container is connected to the first input end of the third three-way valve, and the output end of the standard liquid container is connected to the second input end of the third three-way valve; the output end of the third three-way valve is connected to the first input end of the fourth three-way valve through a peristaltic pump. The output end of the cleaning fluid container is connected to the second input end of the fourth three-way valve; the output end of the fourth three-way valve is connected to the first input end of the water bath temperature control and trapping spiral tube.

5. The hydrogen peroxide content measuring device as described in claim 2, characterized in that, A fifth three-way valve and a peroxide removal device are also provided between the water bath temperature-controlled trapping spiral tube and the gas collection port; The gas collection port is connected to the first input terminal of the fifth three-way valve via a peristaltic pump; the first output terminal of the fifth three-way valve is connected to the second input terminal of the water bath temperature-controlled trapping spiral tube; the second output terminal of the fifth three-way valve is connected to the input terminal of the peroxide removal device; and the output terminal of the peroxide removal device is connected to the second input terminal of the water bath temperature-controlled trapping spiral tube.

6. The hydrogen peroxide content measuring device according to any one of claims 1 to 5, characterized in that, The first fluorescence measurement channel includes a first spiral tube, a first water bath temperature control spiral tube, a first pH adjustment spiral tube, and a first fluorescence detection chamber connected in sequence. The second fluorescence measurement channel includes a second spiral tube, a second water bath temperature-controlled spiral tube, a second pH adjustment spiral tube, and a second fluorescence detection chamber connected in sequence. The input ends of the first and second spiral tubes are used to receive the first test solution and the second test solution, respectively; and the input end of the first spiral tube is connected to a first liquid container via a peristaltic pump; the first liquid container is used to store potassium hydrogen phthalate buffer solution; the input end of the second spiral tube is connected to a second liquid container via a peristaltic pump; the second liquid container is used to store potassium hydrogen phthalate buffer solution containing hydrogen peroxide catalytic enzyme. The input ends of the first water bath temperature-controlled spiral tube and the second water bath temperature-controlled spiral tube are respectively connected to a third liquid container via a peristaltic pump. The third liquid container is used to store a mixture of puerarin and peroxidase. The input ends of the first pH adjusting spiral tube and the second pH adjusting spiral tube are respectively connected to pH adjusting liquid containers via peristaltic pumps; The data processing module is connected to the first fluorescence detection chamber and the second fluorescence detection chamber respectively, and is used to compare the first fluorescence detection data measured by the first fluorescence detection chamber and the second fluorescence detection data measured by the second fluorescence detection chamber to obtain hydrogen peroxide content data.

7. The hydrogen peroxide content measuring device as described in claim 6, characterized in that, The first liquid container, the second liquid container, the third liquid container, and the pH adjustment liquid container are all brown spout bags.

8. The hydrogen peroxide content measuring device as described in claim 6, characterized in that, A bubble venting tee is provided between the first spiral tube and the first water bath temperature-controlled spiral tube, between the first water bath temperature-controlled spiral tube and the first pH adjustment spiral tube, between the first pH adjustment spiral tube and the first fluorescence detection chamber, between the second spiral tube and the second water bath temperature-controlled spiral tube, between the second water bath temperature-controlled spiral tube and the second pH adjustment spiral tube, and between the second pH adjustment spiral tube and the second fluorescence detection chamber.

9. A method for measuring the content of hydrogen peroxide, characterized in that, An apparatus for measuring the content of hydrogen peroxide as described in any one of claims 1 to 8, comprising: The test liquid is obtained by absorbing gaseous hydrogen peroxide from the test sample gas through a gaseous absorption component and / or absorbing hydrogen peroxide particles from the test sample gas through a solid absorption component and / or collecting dissolved hydrogen peroxide from the test sample liquid through a liquid absorption component. The liquid to be tested is divided into a first test liquid and a second test liquid; The first test liquid is subjected to fluorescence detection through the first fluorescence measurement channel to obtain first fluorescence detection data; Hydrogen peroxide is removed from the second test solution through the second fluorescence measurement channel, and fluorescence detection is performed on the second test solution after removing hydrogen peroxide to obtain second fluorescence detection data; The hydrogen peroxide content is obtained by comparing and calculating the first fluorescence detection data and the second fluorescence detection data.

10. The method for measuring hydrogen peroxide content as described in claim 9, characterized in that, It also includes the process of pre-calibrating the concentration response curve; The process of pre-calibrating the concentration response curve includes: Prepare standard solutions of hydrogen peroxide at different concentrations; Each concentration of the hydrogen peroxide standard solution is divided into two portions and passed into the first fluorescence measurement channel and the second fluorescence measurement channel respectively to obtain the first calibration detection data and the second calibration detection data corresponding to each different concentration of the hydrogen peroxide standard solution. The calibration test data of the hydrogen peroxide standard solution is obtained by performing a difference calculation on the first calibration test data and the second calibration test data corresponding to the same concentration of hydrogen peroxide standard solution. A concentration response curve is obtained by linearly fitting the calibration detection data corresponding to the hydrogen peroxide standard solutions of different concentrations and the hydrogen peroxide concentration.

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