Breathing mask device for treating brain glioma through suction type atmospheric pressure low-temperature plasma

Abstract

A breathing mask device for treating brain glioma through suction type atmospheric pressure low-temperature plasma comprises a mask, a catalyst containing channel, a one-way umbrella valve, a discharging channel and an oxygen connector. The invention provides a novel mask device for realizing brain glioma treatment through an inhalation therapy, and the brain glioma treatment can be realized through simple inhalation treatment. In consideration of biocompatibility, a silica gel material is used, and an oxygen input hole is reserved to prevent suffocation. The invention has the beneficial effects of noninvasiveness, high efficiency, multi-mechanism synergistic effect, recurrence risk reduction and comfort.

Description

Technical Field

[0001] This invention relates to the field of tumor treatment technology, specifically to a breathing mask device for inhaling atmospheric pressure low-temperature plasma to treat glioma. Background Technology

[0002] Currently, the main technologies used to treat gliomas include surgical resection, chemotherapy, electric field therapy, immunotherapy, and cold atmospheric plasma (CAP), each with its own characteristics, but there are still many limitations in actual clinical application.

[0003] Surgical intervention: Obtaining tissue for definitive diagnosis, safely removing the tumor to the maximum extent possible, and reducing intracranial pressure. However, traditional surgery is difficult to completely remove highly aggressive gliomas, and the recurrence rate is high.

[0004] Tumor Treating Fields (TTF) therapy utilizes low-intensity electric fields to disrupt the mitotic process of tumor cells, thereby inhibiting their proliferation. It has shown some effectiveness in treating superficial tumors, but its efficacy is significantly limited for deep or extensively infiltrated gliomas due to insufficient penetration depth and uneven field strength distribution.

[0005] Chemotherapy drugs (such as temozolomide): These drugs interfere with the DNA replication and repair mechanisms of tumor cells, thereby killing them. Although drugs like temozolomide can prolong patient survival to some extent, the blood-brain barrier makes it difficult for these drugs to effectively penetrate the brain tumor area, resulting in poor treatment efficacy and significant systemic side effects.

[0006] Immunotherapy: This involves activating or enhancing the patient's own immune system, enabling it to recognize and attack tumor cells. While immunotherapy has made groundbreaking progress in the treatment of certain types of cancer, the immunosuppressive microenvironment and complex immune escape mechanisms of gliomas result in insufficient response to immunotherapy in some patients, limiting its clinical efficacy.

[0007] Cold Atmospheric Plasma (CAP): By generating high concentrations of reactive oxygen / nitrogen species (RONS), CAP induces oxidative stress in tumor cells, thereby achieving an anti-tumor effect. CAP has shown significant anti-tumor potential in in vitro experiments and the treatment of superficial tumors, but due to limitations in current delivery technologies, its application in non-invasive treatment of deep tumors (such as gliomas) has not been fully realized. Summary of the Invention

[0008] The purpose of this invention is to provide a breathing mask device for inhalation-type atmospheric pressure low-temperature plasma therapy for glioma, comprising: a mask, a catalyst placement channel, a one-way umbrella valve, a discharge channel, and an oxygen interface.

[0009] The mask covers the user's mouth and nose area and fits closely to the user's facial skin.

[0010] The mask is provided with an internal chamber for containing plasma active components.

[0011] One end of the catalyst placement channel is connected to the mask, and the other end is connected to the discharge channel.

[0012] The catalyst placement channel is used to place the catalyst.

[0013] The mask is equipped with an exhaust port for discharging the user's exhaled exhaust gas.

[0014] The discharge channel is connected to an external plasma discharge chamber and is used to introduce plasma generated by the external plasma discharge chamber.

[0015] The upper end of the discharge channel is equipped with a one-way umbrella valve for one-way plasma flow.

[0016] The plasma enters the catalyst placement channel through a one-way umbrella valve, and generates plasma active components under the action of the catalyst.

[0017] The plasma active ingredients enter the internal chamber of the mask through the catalyst placement channel.

[0018] The mask has a nasal cannula inside, through which plasma active ingredients are delivered to the user's nasal cavity.

[0019] The oxygen interface is installed on the mask and connected to an external oxygen device to provide oxygen to the user.

[0020] The plasma active ingredients directly reach the glioma microlesions through the olfactory nerve and trigeminal nerve pathways, inducing tumor cell apoptosis.

[0021] Furthermore, the face mask is made of materials including silicone.

[0022] Furthermore, the size of the nasal cannula is adjustable according to the user's nasal cavity structure.

[0023] Furthermore, the external plasma discharge chamber includes a needle electrode, a dielectric, and an external electrode.

[0024] The needle electrode is connected to an external ionization device and receives ionization signals generated by the external ionization device.

[0025] The dielectric surrounds the outside of the needle electrode, isolating the needle electrode from the external electrode.

[0026] The dielectric has a cavity inside, and the two ends of the cavity are connected to a discharge channel and an external gas mixer, respectively.

[0027] The external electrode is grounded and surrounds the outside of the dielectric.

[0028] The mixed gas generated by the external gas mixer passes through the cavity inside the dielectric and generates plasma under the action of an ionization signal.

[0029] Furthermore, the ionization signal includes a square wave pulse and a sinusoidal voltage.

[0030] The square wave pulse has a voltage amplitude range of 6KV-10kV, a pulse width range of 200ns-2000ns, and a frequency range of 5kHz-10kHz.

[0031] The peak-to-peak value range of the sinusoidal voltage is 4kV-10kV, and the frequency range is 5kHz-1000kHz.

[0032] Furthermore, the mixed gas includes at least one of helium, argon, oxygen, and nitrogen.

[0033] Furthermore, the plasma generated in the external plasma discharge chamber is introduced into the discharge channel through a discharge hose.

[0034] The discharge hose includes a polytetrafluoroethylene outer tube and a fine wire electrode.

[0035] The filament electrode is located inside a polytetrafluoroethylene outer tube and is used to transmit plasma.

[0036] Furthermore, the user's state when using the breathing mask device includes inhalation and exhalation.

[0037] Furthermore, when the user is in the inhalation state, the one-way umbrella valve opens and the exhaust port closes, allowing the user to inhale the plasma active ingredients.

[0038] When the user is exhaling, the one-way umbrella valve closes and the exhaust port opens, allowing the user to exhale waste gas.

[0039] Furthermore, the plasma includes .

[0040] The plasma active components include .

[0041] The catalyst includes , Honeycomb carbon blocks , .

[0042] The technical effectiveness of this invention is undeniable. This invention proposes a novel mask device for treating gliomas via inhalation therapy, enabling treatment through simple inhalation. Biocompatibility was considered when using silicone material, and an oxygen inlet was provided to prevent suffocation.

[0043] The beneficial effects of this invention include:

[0044] 1. Non-invasive: The plasma active ingredients are delivered through nasal inhalation, avoiding surgical trauma and resulting in high patient acceptance.

[0045] 2. High efficiency: The active ingredients reach the brain directly through neural pathways, bypassing the blood-brain barrier, which significantly improves the treatment efficiency.

[0046] 3. Synergistic effect of multiple mechanisms: It achieves precise removal of gliomas through multiple mechanisms such as oxidative stress, immune activation and inhibition of tumor angiogenesis.

[0047] 4. Reduce the risk of recurrence: Induces immunogenic cell death, activates anti-tumor immune response, and effectively reduces tumor residue and recurrence.

[0048] 5. Comfort: The breathing mask is made of soft materials and has an adjustable design, which improves patient comfort and treatment compliance. Attached Figure Description

[0049] Figure 1 This is a three-dimensional view of the structure of the present invention;

[0050] Figure 2 This is a structural view of the present invention; Figure 2 (a) is a front view of the structure of the present invention; Figure 2 (b) is a side view of the structure of the present invention;

[0051] Figure 3 This is a schematic diagram of the plasma control delivery system in this invention; Figure 3 (a) is a schematic diagram of the plasma controlled delivery system; Figure 3 (b) is a schematic diagram of the plasma delivery structure inside the human body;

[0052] Figure 4 Workflow diagram for breathing masks;

[0053] Figure 5 A schematic diagram illustrating the multi-stage tumor-killing effect of plasma on gliomas;

[0054] Figure 6 This is a schematic diagram illustrating the therapeutic effect of plasma therapy on mice with gliomas. Figure 6 (a) is a schematic diagram of in vivo imaging of fluorescence intensity in gliomas in the mouse brain; Figure 6 (b) is a statistical graph of fluorescence intensity;

[0055] In the diagram: 1. Mask; 2. Catalyst placement channel; 3. One-way umbrella valve; 4. Discharge channel; 5. Exhaust port; 6. Oxygen interface. Detailed Implementation

[0056] The present invention will be further described below with reference to embodiments, but it should not be construed that the scope of the present invention is limited to the following embodiments. Various substitutions and modifications made based on ordinary technical knowledge and common practices in the art without departing from the above-described technical concept of the present invention should be included within the scope of protection of the present invention.

[0057] Example 1:

[0058] See Figures 1 to 6 A breathing mask device for inhalation-type atmospheric pressure low-temperature plasma therapy for glioma includes: a mask 1, a catalyst placement channel 2, a one-way umbrella valve 3, a discharge channel 4, and an oxygen interface 6.

[0059] The mask 1 covers the user's mouth and nose area and fits closely to the user's facial skin.

[0060] The mask 1 is provided with an internal chamber for containing plasma active components.

[0061] One end of the catalyst placement channel 2 is connected to the mask 1, and the other end is connected to the discharge channel 4.

[0062] The catalyst placement channel 2 is used to place the catalyst.

[0063] The mask 1 is equipped with an exhaust port 5 for discharging the exhaust gas exhaled by the user.

[0064] The discharge channel 4 is connected to an external plasma discharge chamber and is used to introduce plasma generated by the external plasma discharge chamber.

[0065] The upper end of the discharge channel 4 is provided with a one-way umbrella valve 3 for one-way plasma flow.

[0066] The plasma enters the catalyst placement channel 2 through the one-way umbrella valve 3, and generates plasma active components under the action of the catalyst.

[0067] The plasma active components enter the internal chamber of the mask 1 through the catalyst placement channel 2.

[0068] The mask 1 is equipped with a nasal cannula, through which plasma active ingredients are delivered to the user's nasal cavity.

[0069] The oxygen interface 6 is installed on the mask 1 and connected to an external oxygen device to provide oxygen to the user.

[0070] The plasma active ingredients directly reach the glioma microlesions through the olfactory nerve and trigeminal nerve pathways, inducing tumor cell apoptosis.

[0071] Example 2:

[0072] A respiratory mask device for inhalation-type atmospheric pressure low-temperature plasma therapy for glioma is described in Example 1. Further, the material used for the mask 1 includes silicone.

[0073] Example 3:

[0074] A breathing mask device for inhaling atmospheric pressure low-temperature plasma therapy for glioma, the main technical contents of which are described in any one of Embodiments 1 to 2, further wherein the size of the nasal cannula is adjustable according to the user's nasal cavity structure.

[0075] Example 4:

[0076] A breathing mask device for inhaling atmospheric pressure low-temperature plasma to treat glioma, the main technical contents of which are described in any one of Examples 1 to 3. Further, the external plasma discharge chamber includes a needle electrode, a dielectric, and an external electrode.

[0077] The needle electrode is connected to an external ionization device and receives ionization signals generated by the external ionization device.

[0078] The dielectric surrounds the outside of the needle electrode, isolating the needle electrode from the external electrode.

[0079] The dielectric has a cavity inside, and the two ends of the cavity are connected to the discharge channel 4 and the external gas mixer, respectively.

[0080] The external electrode is grounded and surrounds the outside of the dielectric.

[0081] The mixed gas generated by the external gas mixer passes through the cavity inside the dielectric and generates plasma under the action of an ionization signal.

[0082] Example 5:

[0083] A breathing mask device for inhaling atmospheric pressure low-temperature plasma to treat glioma, the main technical contents of which are described in any one of Examples 1 to 4, further wherein the ionization signal includes a square wave pulse and a sinusoidal voltage.

[0084] The square wave pulse has a voltage amplitude range of 6KV-10kV, a pulse width range of 200ns-2000ns, and a frequency range of 5kHz-10kHz.

[0085] The peak-to-peak value range of the sinusoidal voltage is 4kV-10kV, and the frequency range is 5kHz-1000kHz.

[0086] Example 6:

[0087] A breathing mask device for inhalation-type atmospheric pressure low-temperature plasma therapy for glioma, the main technical contents of which are described in any one of Examples 1 to 5, further wherein the mixed gas includes at least one of helium, argon, oxygen and nitrogen.

[0088] Example 7:

[0089] A breathing mask device for inhaling atmospheric pressure low-temperature plasma to treat glioma, the main technical contents of which are described in any one of Examples 1 to 6, further wherein the plasma generated by the external plasma discharge chamber is introduced into the discharge channel 4 through a discharge hose.

[0090] The discharge hose includes a polytetrafluoroethylene outer tube and a fine wire electrode.

[0091] The filament electrode is located inside a polytetrafluoroethylene outer tube and is used to transmit plasma.

[0092] Example 8:

[0093] A breathing mask device for inhalation of atmospheric pressure low-temperature plasma for the treatment of glioma, the main technical contents of which are described in any one of Embodiments 1 to 7. Furthermore, the state of the user when using the breathing mask device includes inhalation state and exhalation state.

[0094] Example 9:

[0095] A breathing mask device for inhaling atmospheric pressure low-temperature plasma to treat glioma, the main technical contents of which are described in any one of Examples 1 to 8. Further, when the user is in the inhalation state, the one-way umbrella valve 3 is opened and the exhaust port 5 is closed, and the user inhales the active ingredients of the plasma.

[0096] When the user is exhaling, the one-way umbrella valve 3 closes and the exhaust port 5 opens, allowing the user to exhale exhaust gas.

[0097] Example 10:

[0098] A respiratory mask device for inhalation-type atmospheric pressure low-temperature plasma therapy for glioma, the main technical contents of which are described in any one of Examples 1 to 9, further wherein the plasma includes .

[0099] The plasma active components include .

[0100] The catalyst includes , Honeycomb carbon blocks , .

[0101] Example 11:

[0102] See Figures 1 to 6 A breathing mask device for inhalation-type atmospheric pressure low-temperature plasma therapy for glioma includes: a mask 1, a catalyst placement channel 2, a one-way umbrella valve 3, a discharge channel 4, and an oxygen interface 6.

[0103] The mask 1 covers the user's mouth and nose area and fits closely to the user's facial skin.

[0104] The mask 1 is provided with an internal chamber for containing plasma active components.

[0105] One end of the catalyst placement channel 2 is connected to the mask 1, and the other end is connected to the discharge channel 4.

[0106] The catalyst placement channel 2 is used to place the catalyst.

[0107] The mask 1 is equipped with an exhaust port 5 for discharging the exhaust gas exhaled by the user.

[0108] The discharge channel 4 is connected to an external plasma discharge chamber and is used to introduce plasma generated by the external plasma discharge chamber.

[0109] The upper end of the discharge channel 4 is provided with a one-way umbrella valve 3 for one-way plasma flow.

[0110] The plasma enters the catalyst placement channel 2 through the one-way umbrella valve 3, and generates plasma active components under the action of the catalyst.

[0111] The plasma active components enter the internal chamber of the mask 1 through the catalyst placement channel 2.

[0112] The mask 1 is equipped with a nasal cannula, through which plasma active ingredients are delivered to the user's nasal cavity.

[0113] The oxygen interface 6 is installed on the mask 1 and connects to an external oxygen device to provide oxygen to the user. It maintains the oxygen concentration inside the mask to ensure the patient's respiratory safety during treatment.

[0114] The plasma active ingredients directly reach the glioma microlesions through the olfactory nerve and trigeminal nerve pathways, inducing tumor cell apoptosis.

[0115] Working principle:

[0116] A mixed gas (helium, oxygen, nitrogen, etc.) enters the plasma discharge chamber through a mass flow controller and is ionized under high voltage to generate high concentrations of reactive oxygen / nitrogen species (RONS).

[0117] The active ingredient is delivered to the breathing mask through a discharge tube and inhaled by the patient through the nasal cavity. The active ingredient directly reaches the microlesion of the glioma via the olfactory nerve and trigeminal nerve pathway.

[0118] The active ingredients induce tumor cell apoptosis, autophagy, necrosis and pyroptosis through oxidative stress, while activating the immune response and inhibiting tumor invasion and recurrence.

[0119] Example 12:

[0120] A respiratory mask device for inhalation of atmospheric pressure low-temperature plasma for the treatment of gliomas is described in Example 11. Further, the material used for the mask 1 includes silicone.

[0121] Example 13:

[0122] A breathing mask device for inhaling atmospheric pressure low-temperature plasma therapy for glioma, the main technical contents of which are described in any one of Examples 11 to 12, further wherein the size of the nasal cannula is adjustable according to the user's nasal cavity structure.

[0123] The nasal cannula is designed to be adjustable to adapt to the nasal cavity structure of different patients, ensuring that the plasma active ingredients can be efficiently delivered to the nasal cavity.

[0124] Example 14:

[0125] A breathing mask device for inhaling atmospheric pressure low-temperature plasma to treat glioma, the main technical contents of which are described in any one of Examples 11 to 13. Further, the external plasma discharge chamber includes a needle electrode, a dielectric, and an external electrode.

[0126] The needle electrode is connected to an external ionization device and receives ionization signals generated by the external ionization device.

[0127] The dielectric surrounds the outside of the needle electrode, isolating the needle electrode from the external electrode.

[0128] The dielectric has a cavity inside, and the two ends of the cavity are connected to the discharge channel 4 and the external gas mixer, respectively.

[0129] The external electrode is grounded and surrounds the outside of the dielectric.

[0130] The mixed gas generated by the external gas mixer passes through the cavity inside the dielectric and generates plasma under the action of an ionization signal.

[0131] Example 15:

[0132] A breathing mask device for inhaling atmospheric pressure low-temperature plasma to treat glioma, the main technical contents of which are described in any one of Examples 11 to 14. Further, the ionization signal includes a high-voltage square wave pulse with a pulse width of 10kV, a repetition frequency of 500ns, and a sinusoidal voltage with an amplitude of 4kV and a frequency of 5kHz.

[0133] The square wave pulse has a voltage amplitude range of 6KV-10kV, a pulse width range of 200ns-2000ns, and a frequency range of 5kHz-10kHz.

[0134] The peak-to-peak value range of the sinusoidal voltage is 4kV-10kV, and the frequency range is 5kHz-1000kHz.

[0135] Example 16:

[0136] A breathing mask device for inhalation-type atmospheric pressure low-temperature plasma therapy for glioma, the main technical contents of which are described in any one of Examples 11 to 15, further wherein the mixed gas includes at least one of helium, argon, oxygen and nitrogen.

[0137] Example 17:

[0138] A breathing mask device for inhaling atmospheric pressure low-temperature plasma to treat glioma, the main technical contents of which are described in any one of Examples 11 to 16, further wherein the plasma generated by the external plasma discharge chamber is introduced into the discharge channel 4 through a discharge hose.

[0139] The discharge hose includes a 3mm diameter polytetrafluoroethylene outer tube and a 0.8mm diameter filament electrode.

[0140] The filament electrode is located inside a polytetrafluoroethylene outer tube and is used to transmit plasma.

[0141] Example 18:

[0142] A breathing mask device for inhaling atmospheric pressure low-temperature plasma therapy for glioma, the main technical contents of which are described in any one of Examples 11 to 17. Furthermore, the user's state when using the breathing mask device includes an inhalation state and an exhalation state.

[0143] Example 19:

[0144] A breathing mask device for inhaling atmospheric pressure low-temperature plasma to treat glioma, the main technical contents of which are described in any one of Examples 11 to 18. Further, when the user is in the inhalation state, the one-way umbrella valve 3 is opened and the exhaust port 5 is closed, and the user inhales the plasma active ingredients.

[0145] When the user is exhaling, the one-way umbrella valve 3 closes and the exhaust port 5 opens, allowing the user to exhale exhaust gas.

[0146] Example 20:

[0147] A respiratory mask device for inhalation-type atmospheric pressure low-temperature plasma therapy for glioma, the main technical contents of which are described in any one of Examples 11 to 19, further wherein the plasma includes .

[0148] The plasma active components include .

[0149] The catalyst includes , Honeycomb carbon blocks , .

[0150] Example 21:

[0151] See Figures 1 to 6 A respiratory mask device for inhalation-type atmospheric pressure low-temperature plasma therapy of glioma, the main technical contents of which include:

[0152] The structure of a breathing mask device:

[0153] The main body of the mask is made of soft, biocompatible materials to ensure wearing comfort and a tight seal.

[0154] Gas interface: Connects the plasma discharge chamber and the breathing mask to ensure that the active components of the plasma can smoothly enter the mask.

[0155] Nasal cannula: Designed to be adjustable to adapt to different patients' nasal cavity structures, ensuring that plasma active ingredients can be efficiently delivered to the nasal cavity.

[0156] Oxygen inlet: Maintains the oxygen concentration within the mask, ensuring the patient's respiratory safety during treatment. Working principle:

[0157] Working principle:

[0158] A mixed gas (helium, oxygen, nitrogen, etc.) enters the plasma discharge chamber through a mass flow controller and is ionized under high voltage to generate high concentrations of reactive oxygen / nitrogen species (RONS).

[0159] The active ingredient is delivered to the breathing mask through a discharge tube and inhaled by the patient through the nasal cavity. The active ingredient directly reaches the microlesion of the glioma via the olfactory nerve and trigeminal nerve pathway.

[0160] The active ingredients induce tumor cell apoptosis, autophagy, necrosis and pyroptosis through oxidative stress, while activating the immune response and inhibiting tumor invasion and recurrence.

[0161] Example 22:

[0162] See Figures 1 to 6 A respiratory mask device for inhalation-type atmospheric pressure low-temperature plasma therapy of glioma, the main technical contents of which include:

[0163] See appendix Figure 1 and attached Figure 2 This document outlines the components and workflow of a plasma-assisted glioma treatment respiratory mask device. 1. Mask: Made of soft, biocompatible material. 2. Catalyst placement channel: Used to place the catalyst. 3. One-way umbrella valve: Controls unidirectional gas flow. 4. Discharge channel: Used to generate plasma.

[0164] See appendix Figure 3 This invention relates to a system and control method for plasma generation and delivery. The system includes a working gas source, a carrier gas connection, a working gas mixer and its output, a plasma discharge chamber for ionizing the mixed gas into plasma, and an oxygen input connected to maintain the oxygen concentration within a breathing mask. The working gas may include at least one of helium, argon, nitrogen, and oxygen. One or more working gases flow through a mass flow controller (MFC), which controls the ratio of rare gas doping (oxygen and nitrogen) and the flow rate of the mixed working gas. A high-precision MFC can control the mixed gas flow rate within the range of 0.5 L / min to 10 L / min, with the oxygen and nitrogen doping ratio typically set to 1000 ppm. The mixed working gas is connected to the plasma discharge chamber via a quick-connect interface. The plasma discharge chamber has an inner chamber surrounded by needle electrodes and a dielectric, and an outer electrode connected to ground, forming a dielectric barrier discharge system for generating plasma. The mixture of the first and second working gases is ionized by a high-voltage square wave pulse with a pulse width of 10 kV, a repetition frequency in the kHz range, or a sinusoidal voltage with an amplitude of 4 kV and a frequency of 5 kHz to generate active particles. The active particles are then inserted into the inner tube of the breathing mask via a discharge hose. The discharge hose consists of a 3 mm diameter polytetrafluoroethylene outer tube and a 0.8 mm diameter fine wire electrode, which delivers the plasma from the discharge chamber to a distal end and generates a large volume of plasma at the active end of the discharge hose.

[0165] Figure 4This invention demonstrates the process of pulsed plasma diffusion delivery to a breathing mask. By precisely controlling the gas flux, a mixed working gas is introduced into the system to ensure that the medium environment for plasma delivery has an appropriate gas composition and concentration. A multi-parameter adjustable high-voltage generator ionizes and generates a high concentration of active material. A single-way breathing valve detects the breathing state. If the patient is inhaling, the plasma channel valve opens to draw in the active ingredient, while the exhaust port closes to ensure effective absorption of the plasma active ingredient. If the patient is exhaling, the gas valve closes to ensure gas stability in the plasma discharge region. Simultaneously, the exhaust port opens to expel waste gas.

[0166] Figure 5 This invention demonstrates the multi-stage killing effect induced by pulsed plasma diffusion delivery to gliomas.

[0167] First, the reactive substances produced after ionization diffuse across the blood-brain barrier and then act on the glioma via the cerebrospinal fluid. The basal levels of these reactive substances in tumor cells are much higher than in normal cells, making them more susceptible to exceeding the reactive substance tolerance threshold. Oxidative stress damage to tumor tissue further induces programmed cell death, leading to apoptosis, autophagy, necrosis, and pyroptosis in cancer cells. On the other hand, the cancer cell death induced by both gaseous and liquid reactive substances releases tumor-associated antigens and promotes the maturation of dendritic cells (DCs) in the tumor-draining lymph nodes. DCs can display major histocompatibility complex peptides to T cells. This subsequently initiates a T-cell-mediated immune response; a strong T-cell-mediated immune response can combat residual tumor cells, ultimately achieving tumor suppression.

[0168] Figure 6 This is a schematic diagram illustrating the therapeutic effect of plasma on gliomas in mice. The left image shows an in vivo imaging image of the fluorescence intensity of gliomas in the mouse brain. Compared to the control group, the fluorescence intensity of gliomas in the plasma group was significantly reduced. The right image is a statistical graph of fluorescence intensity, clearly showing that after plasma inhalation, the fluorescence intensity of tumors in the plasma group was significantly reduced, indicating that the tumor volume was significantly smaller than that in the control group. The parameters used were a square wave pulse of 7kV, 10kHz, and 1us pulse width, continuously treated for 5 minutes, once a day for five consecutive days, from Day 7 to Day 11, followed by in vivo imaging observation.

[0169] Example 23:

[0170] A breathing mask device for inhalation of atmospheric pressure low-temperature plasma for the treatment of gliomas is described in Example 22. Further, the gas flow rate is simplified: the flow rate of the working gas is simplified from 5L / min to 2L / min, while still achieving effective treatment and reducing the complexity of the equipment.

[0171] Example 24:

[0172] A breathing mask device for inhaling atmospheric pressure low-temperature plasma therapy for glioma, the main technical contents of which are described in any one of Examples 22 to 23. Further, the discharge mode is replaced: the high-voltage square wave pulse is replaced with a high-voltage sine voltage. After adjusting the voltage frequency, a high concentration of active ingredients can still be generated, simplifying the power supply design.

[0173] Example 25:

[0174] A breathing mask device for inhalation-type atmospheric pressure low-temperature plasma therapy for glioma, the main technical contents of which are described in any one of Examples 22 to 24. Further, the breathing mask is optimized by adopting an adjustable mask design to adapt to different patients' nasal cavity structures, thereby improving treatment comfort and delivery efficiency.

[0175] Example 26:

[0176] A respiratory mask device for inhalation of atmospheric pressure low-temperature plasma for the treatment of glioma, the main technical contents of which are described in any one of Examples 22 to 25, further, material improvement: the mask is made of a material with higher biocompatibility to reduce nasal irritation or allergic reactions that may be caused by long-term use.

Claims

1. A respiratory mask device for inhalation-type atmospheric pressure low-temperature plasma therapy of glioma, characterized in that, include: Mask (1), catalyst placement channel (2), one-way umbrella valve (3), discharge channel (4), oxygen interface (6); The mask (1) covers the user's mouth and nose area and fits closely to the user's facial skin; The mask (1) is provided with an internal chamber for containing plasma active components; One end of the catalyst placement channel (2) is connected to the mask (1), and the other end is connected to the discharge channel (4); The catalyst placement channel (2) is used to place the catalyst; The mask (1) is provided with an exhaust port (5) for discharging the exhaust gas exhaled by the user; The discharge channel (4) is connected to the external plasma discharge chamber and is used to introduce the plasma generated by the external plasma discharge chamber; The upper end of the discharge channel (4) is provided with a one-way umbrella valve (3) for one-way plasma conduction; The plasma enters the catalyst placement channel (2) through the one-way umbrella valve (3) and generates plasma active components under the action of the catalyst; The plasma active components enter the internal chamber of the mask (1) through the catalyst placement channel (2); The mask (1) is equipped with a nasal cannula, through which plasma active ingredients are delivered to the user's nasal cavity; The oxygen interface (6) is installed on the mask (1) and connected to an external oxygen device to provide oxygen to the user; The plasma active ingredients directly reach the glioma microlesions through the olfactory nerve and trigeminal nerve pathways, inducing tumor cell apoptosis.

2. The breathing mask device for inhalation-type atmospheric pressure low-temperature plasma therapy for glioma according to claim 1, characterized in that, The material used for the face mask (1) includes silicone.

3. The inhalation mask device for treating glioma using inhaled atmospheric pressure low-temperature plasma according to claim 1, characterized in that, The size of the nasal cannula is adjustable according to the user's nasal cavity structure.

4. The breathing mask device for inhalation-type atmospheric pressure low-temperature plasma therapy for glioma according to claim 1, characterized in that, The external plasma discharge chamber includes a needle electrode, a dielectric, and an external electrode; The needle electrode is connected to an external ionization device and receives the ionization signal generated by the external ionization device. The dielectric surrounds the outside of the needle electrode, isolating the needle electrode from the outer electrode; The dielectric has a cavity inside, and the two ends of the cavity are connected to the discharge channel (4) and the external gas mixer, respectively. The outer electrode is grounded and surrounds the outside of the dielectric; The mixed gas generated by the external gas mixer passes through the cavity inside the dielectric and generates plasma under the action of an ionization signal.

5. The inhalation mask device for treating glioma using inhaled atmospheric pressure low-temperature plasma according to claim 4, characterized in that, The ionization signal includes square wave pulses and sinusoidal voltages; The square wave pulse has a voltage amplitude range of 6KV-10kV, a pulse width range of 200ns-2000ns, and a frequency range of 5kHz-10kHz. The peak-to-peak value range of the sinusoidal voltage is 4kV-10kV, and the frequency range is 5kHz-1000kHz.

6. The inhalation mask device for treating glioma using inhaled atmospheric pressure low-temperature plasma according to claim 4, characterized in that, The mixed gas includes at least one of helium, argon, oxygen, and nitrogen.

7. The breathing mask device for inhalation-type atmospheric pressure low-temperature plasma therapy for glioma according to claim 4, characterized in that, The plasma generated by the external plasma discharge chamber is introduced into the discharge channel (4) through the discharge hose. The discharge hose includes a polytetrafluoroethylene outer tube and a fine wire electrode; The filament electrode is located inside a polytetrafluoroethylene outer tube and is used to transmit plasma.

8. The inhalation mask device for treating glioma using atmospheric pressure low-temperature plasma according to claim 1, characterized in that, The user's state when using the breathing mask device includes inhalation and exhalation.

9. A respiratory mask device for inhalation-type atmospheric pressure low-temperature plasma therapy for glioma according to claim 8, characterized in that, When the user is in the inhalation state, the one-way umbrella valve (3) opens and the exhaust port (5) closes, and the user inhales the plasma active ingredients; When the user is exhaling, the one-way umbrella valve (3) closes and the exhaust port (5) opens, allowing the user to exhale exhaust gas.

10. The inhalation mask device for treating glioma using atmospheric pressure low-temperature plasma according to claim 1, characterized in that, The plasma includes ; The plasma active components include ; The catalyst includes , Honeycomb carbon blocks , .

Images