Preparation method and application of katsumadai katsumadai extract

Abstract

The invention relates to the technical field of daily cosmetics, and particularly provides a preparation method and application of a katsumadai katsumadai extract. In the preparation process of the alpinia katsumadai extract, an ethanol / water mixed solvent (preferably 75 + / -5% (v / v)) with a specific concentration is used as an extraction solvent, the solvent is safe and non-toxic, and the alpinia katsumadai extract obtained by enrichment after purification through macroporous resin (preferably ADS-17) has excellent dual UV absorption performance of absorbing UVA and absorbing UVB; the alpinia katsumadai extract disclosed by the invention is a plant component, has the advantages of high safety, low irritation, no cell damage, no influence on cell viability, extremely small or no side effect, wide applicable crowd, and is very suitable for being used as an active component of cosmetics, and is particularly suitable for being used for preparing sunscreen cosmetics.

Description

Technical Field

[0001] This invention belongs to the field of daily cosmetics technology. Specifically, this invention relates to a method for preparing cardamom extract and its application. Background Technology

[0002] The depletion of the ozone layer has led to a more severe threat of ultraviolet (UV) damage to humans, including UVB (280–320 nm) and UVA (320–400 nm). UVB has high energy and can cause severe photodamage to the skin, leading to redness, blisters, and in severe cases, chromosome damage, immune system suppression, and skin cancer. UVA has strong penetrating power, reaching deep into the dermis and causing irreversible damage, resulting in melanin deposition and skin aging. Therefore, broad-spectrum sunscreens that protect against both UVB and UVA have become a focus of demand.

[0003] Huang Liuqing et al. tested 239 batches of sunscreen products, and found that 3 batches had sunscreen agents that did not meet the technical requirements stated on the label or registration documents. Sunscreen products often use a combination of several sunscreen agents, with the most common samples containing 4 or 5 sunscreen agents. A total of 17 sunscreen agents were detected, with ethylhexyl methoxycinnamate being the most frequently used. Currently, the market mainly achieves broad-spectrum sun protection through the combination of chemical sunscreens with UVB and UVA agents, but chemical sunscreens have high allergy rates, strong irritation, and their safety is questionable.

[0004] Currently, research on plant-based sunscreen ingredients mainly focuses on traditional Chinese medicine. However, these products generally suffer from shortcomings such as narrow UV protection bands, insufficient absorption intensity, dark product colors, difficulties in standardized production, and high costs of some raw materials, making them difficult to commercialize.

[0005] Therefore, there is an urgent need in this field to develop a broad-spectrum sunscreen product that simultaneously protects against UVB and UVA and has high safety. Summary of the Invention

[0006] The purpose of this invention is to provide a method for preparing cardamom extract and its application in the fields of sun protection and beauty.

[0007] In a first aspect of the present invention, a method for preparing a broad-spectrum sunscreen extract of cardamom is provided, the method comprising the steps of: (S1) Extraction: The cardamom seed raw material was extracted with an ethanol / water mixed solvent to obtain an ethanol / water extract; (S2) Purification: The alcohol / water extract obtained in step (S1) is purified by macroporous resin. The purification process includes: loading the sample into the macroporous resin for resin adsorption, eluting with water, and then eluting with ethanol solution. The ethanol solution eluent is collected to obtain an eluent containing cardamom extract. (S3) The solution containing cardamom extract is concentrated to obtain cardamom extract; The macroporous resin is selected from the group consisting of ADS-17, AB-8, HP-20, or NKA-9, or a combination thereof.

[0008] In another preferred embodiment, the volume concentration of ethanol in the ethanol / water mixed solvent is 50-90% (v / v), more preferably 60-85% (v / v), and even more preferably 65-80% (v / v).

[0009] In another preferred embodiment, the ethanol solution used for analysis is an aqueous solution of ethanol.

[0010] In another preferred embodiment, the ethanol solution used for analysis has a volume concentration of 60-85% (v / v), more preferably 65-80% (v / v), and even more preferably 75±5% (v / v), with the balance being water.

[0011] In another preferred embodiment, the macroporous resin is ADS-17, AB-8, or NKA-9; preferably, it is ADS-17.

[0012] In another preferred embodiment, the method includes the steps of: (S1) Extraction: Extract the cardamom seed raw material (powder) with ethanol / water mixed solvent, collect the filtrate and concentrate it to obtain cardamom extract crude extract or concentrated solution; (S2) Purification: Take the crude extract or concentrate obtained in step (S1), after optional dilution, load it onto a macroporous resin for resin adsorption, elute with water, and then elute with ethanol solution. Collect the ethanol solution eluent to obtain the eluent containing cardamom extract. (S3) The solution containing cardamom extract is concentrated to obtain cardamom extract.

[0013] In another preferred embodiment, the method further includes step (S4): freeze-drying or dissolving the cardamom extract obtained in step (S3) in a polyol mixture to obtain the sunscreen formulation containing the cardamom extract.

[0014] In another preferred embodiment, step (S4) further includes a sub-step: (S4a) The cardamom extract obtained in step (S3) is vacuum dried or freeze-dried to obtain a powder containing cardamom extract.

[0015] In another preferred embodiment, step (S4) further includes a sub-step: (S4b) The cardamom extract obtained in step (S3) is dissolved in a polyol mixture to obtain a cardamom extract containing the cardamom extract.

[0016] In another preferred embodiment, step (S1) includes the following sub-steps: (S1a) Provide cardamom seed raw material (powder), add ethanol / water mixed solvent with a concentration of 75±5 %v / v for extraction, collect the extract, concentrate, and obtain cardamom extract crude extract or concentrate.

[0017] In another preferred embodiment, step (S2) includes the following sub-steps: (S2a) Dilute the crude extract or concentrate of cardamom extract to obtain the loading solution; (S2b) The sample solution is loaded onto a macroporous resin for resin adsorption and purification. The purification process includes: loading the sample into a macroporous resin for resin adsorption, eluting with water, then eluting with an ethanol solution, collecting the ethanol solution eluent, thereby obtaining an eluent containing cardamom extract.

[0018] In another preferred embodiment, step (S3) further includes the following sub-steps: (S3a) The solution is concentrated, combined and dried to obtain cardamom extract.

[0019] In another preferred embodiment, the volume ratio of the crude extract of cardamom to the macroporous resin is 1:(1-5).

[0020] In another preferred embodiment, the volume ratio of the crude extract of cardamom to the macroporous resin is 1:(3-5).

[0021] In another preferred embodiment, the water elution flow rate in step (S2b) of the method is 3-5 BV / h.

[0022] In another preferred embodiment, step (S2b) of the method involves collecting an ethanol solution eluent corresponding to an alcohol washing flow rate of 1-4 BV / h.

[0023] In another preferred embodiment, the cardamom seeds described in step (S1) are derived from cardamom fruits selected from the group consisting of: young cardamom fruits, mature cardamom fruits, or combinations thereof; preferably mature fruits.

[0024] In another preferred embodiment, the extraction in step (S1) is a heating reflux extraction.

[0025] In another preferred embodiment, the reflux extraction time in step (S1) is 1.5-2.5 hours.

[0026] In another preferred embodiment, the mass ratio of the crude extract to the macroporous resin in step (S2) is 100%-250%.

[0027] In another preferred embodiment, the eluent containing cardamom extract has a UVA retention rate F1 ≥ 55%, more preferably F1 ≥ 60%.

[0028] In another preferred embodiment, the eluent containing cardamom extract has a UVB retention rate F1 ≥ 45%, more preferably F1 ≥ 50%.

[0029] In another preferred embodiment, the retention rate F1 is calculated using the following formula Q1: F1 = Fx × Fj (Q1) In the formula, Fx represents the adsorption rate of the macroporous resin on the active substances in cardamom extract. Fj represents the desorption rate of the active ingredients in cardamom extract obtained by ethanol solution from macroporous resin. Furthermore, the adsorption rate and desorption rate are measured at the same absorption wavelength for either UVA or UVB.

[0030] In another preferred embodiment, the absorption wavelength of the UVA is selected from 280-310 nm (preferably 290 nm); In another preferred embodiment, the absorption wavelength of the UVB is selected from 315-400 nm (preferably 350 nm).

[0031] In another preferred embodiment, the adsorption rate Fx is calculated using the following formula Q2: Fx = [(W 浸膏- W 水 ) / W 浸膏 ]×100% Q2 In the formula: W 水 This refers to the amount or absorbance of the active ingredient in the water wash buffer after sample loading. W 浸膏 This refers to the amount or absorbance of active ingredients in the crude extract of cardamom and nutmeg extract.

[0032] In another preferred embodiment, the desorption rate Fj is calculated according to the following formula Q3: Fj = [W 醇 / (W 浸膏- W 水 )]×100% Q3 In the formula: W 醇 This refers to the amount of active material or absorbance of the ethanol eluent. W 浸膏 The amount or absorbance of active ingredients in the crude extract of cardamom buds; W 水 This refers to the amount or absorbance of the active ingredient in the water wash buffer after sample loading.

[0033] In a second aspect of the present invention, a broad-spectrum sunscreen cardamom extract is provided, wherein the cardamom extract is obtained by extracting cardamom seeds as raw material through an ethanol / water mixed solvent and then purifying them through a macroporous resin. Furthermore, the cardamom extract described herein possesses dual UV absorption properties, absorbing both UVA and UVB.

[0034] In another preferred embodiment, the cardamom extract has an absorbance A1 of 1.20-2.0 for UVA, more preferably 1.25-1.85, and an absorbance A2 of 0.9-2.0 for UVB, more preferably 0.95-1.8.

[0035] In another preferred embodiment, the cardamom extract has an absorbance A1 of 1.45-1.82 for UVA and an absorbance A2 of 1.35-1.70 for UVB.

[0036] In another preferred embodiment, the sum of the absorbance A1 of the cardamom extract against UVA and the absorbance A2 against UVB is A totol The range is 2.0-3.8, better is 2.2-3.6, even better is 2.4-3.6, and optimal is 3.3-3.6.

[0037] In another preferred embodiment, the determination method is carried out as follows: take an extract (crude extract, paste or extract purified by macroporous resin) corresponding to 0.1g of cardamom raw material (dry weight), add water to make up to 100ml, obtain a test sample (i.e. a sample diluted by 1000), and determine the absorbance by spectral scanning method of an ultraviolet spectrophotometer.

[0038] In another preferred embodiment, under the same test conditions, the direct water extract (without post-treatment) obtained by using water as a solvent has an absorbance of about 0.118 for UVA and about 0.069 for UVB.

[0039] In another preferred embodiment, the ratio Z1 of the absorbance A1 to A1w of the cardamom extract against UVA is ≥10, preferably ≥12, and more preferably ≥15, compared to the absorbance A1w of the direct aqueous extract obtained by using water as a solvent.

[0040] In another preferred embodiment, the ratio Z1 of the absorbance A2 to A2w of the cardamom extract against UVB is ≥18, preferably ≥22, and more preferably ≥25, compared to the absorbance A2w of the direct aqueous extract obtained by using water as a solvent.

[0041] In another preferred embodiment, the cardamom extract is prepared using the method described in the first aspect of the invention.

[0042] In another preferred embodiment, the volume concentration of ethanol in the ethanol / water mixed solvent is 50-90% (v / v), more preferably 60-85% (v / v), and even more preferably 65-80% (v / v).

[0043] In another preferred embodiment, the ethanol solution used for analysis is an aqueous solution of ethanol.

[0044] In another preferred embodiment, the ethanol solution used for analysis has a volume concentration of 60-85% (v / v), more preferably 65-80% (v / v), and even more preferably 75±5% (v / v), with the balance being water.

[0045] In another preferred embodiment, the macroporous resin is selected from the group consisting of ADS-17, AB-8, HP-20, or NKA-9, or combinations thereof.

[0046] In another preferred embodiment, the macroporous resin is ADS-17, AB-8, or NKA-9; more preferably, it is ADS-17.

[0047] In another preferred embodiment, the concentration of the ethanol solution used for analysis is 60-85% (v / v), more preferably 75±5% (v / v).

[0048] In a third aspect of the invention, a use is provided for a cardamom extract as described in the second aspect of the invention, said extract being used to prepare daily or cosmetic products for protection against UVA and UVB.

[0049] In another preferred embodiment, the product is used for sun protection.

[0050] In another preferred embodiment, the product has a sun protection factor (SPF) value ≥ 6.76.

[0051] In another preferred embodiment, the product further has one or more features selected from the group consisting of: (1) Sun protection factor (SPF) value > 6.76; (2) PFA > 5.12; (3) Critical wavelength ≥380nm, GP***, with broad spectrum.

[0052] In a fourth aspect of the invention, a composition for protection against UVA and UVB is provided, the composition comprising: (a) Cardamom extract as described in the second aspect of the invention; and (b) A pharmaceutically or cosmetically acceptable carrier.

[0053] In another preferred embodiment, the composition is a cosmetic composition.

[0054] In another preferred embodiment, the dosage form of the composition is a solid dosage form, a semi-solid dosage form, or a liquid dosage form, such as a solution, gel, emulsion, spray, ointment, cream (such as sunscreen), paste, cake, powder, patch, etc.

[0055] In another preferred embodiment, the composition is a sunscreen composition.

[0056] In another preferred embodiment, the composition is a cream or emulsion.

[0057] In another preferred embodiment, the cosmetic composition is selected from the group consisting of: face cream, skin lotion, makeup base, lotion, face mask, and sunscreen.

[0058] In another preferred embodiment, the composition is used for purposes selected from the group consisting of: sun protection, whitening, or repairing the skin barrier, or combinations thereof.

[0059] In another preferred embodiment, the content of cardamom extract in the composition is 0.0001-15 wt%, more preferably 0.0001-10 wt% or 0.001-5 wt%, such as 0.01 wt%, 0.1 wt%, 0.2 wt%, 0.5 wt%, 1 wt%, 2 wt% or 4 wt%, based on the total weight of the composition.

[0060] It should be understood that, within the scope of this invention, the above-described technical features of this invention and the technical features specifically described below (such as in the embodiments) can be combined with each other to form new or preferred technical solutions. Due to space limitations, they will not be described in detail here. Attached Figure Description

[0061] Figure 1 The spectral scan of the cardamom extract after extraction and dilution using water as a solvent is shown.

[0062] Figure 2 The spectral scan of the cardamom extract extracted and diluted using 60% (v / v) aqueous ethanol as solvent is shown.

[0063] Figure 3 The spectral scan of the cardamom extract extracted and diluted using 75% (v / v) aqueous ethanol as solvent is shown.

[0064] Figure 4 The spectral scan of the cardamom extract extracted and diluted using 85% (v / v) aqueous ethanol as solvent is shown.

[0065] Figure 5 The spectrum of the cardamom extract after extraction and dilution using an aqueous solution of an ionic liquid (betaine, glycerol, propylene glycol in a mass ratio of 20:88:2) as the solvent is shown.

[0066] Figure 6 The image shows a comparison of the spectral scans of cardamom extract extracted with different solvents (including water, 60% (v / v) aqueous ethanol, 75% (v / v) aqueous ethanol, 85% (v / v) aqueous ethanol and ionic liquid aqueous solution).

[0067] Figure 7 The spectrum of the unadsorbed cardamom extract after purification with macroporous resin AB-8 is shown.

[0068] Figure 8 The spectrum of the unadsorbed cardamom extract after purification with macroporous resin D101 is shown.

[0069] Figure 9 The spectrum of the unadsorbed cardamom extract after purification with macroporous resin NKA-9 is shown.

[0070] Figure 10 The spectrum of the unadsorbed cardamom extract after purification with macroporous resin HPD-100 is shown.

[0071] Figure 11 The spectrum of the unadsorbed cardamom extract after purification with macroporous resin HPD-600 is shown.

[0072] Figure 12 The spectrum of the unadsorbed cardamom extract after purification with macroporous resin HP-20 is shown.

[0073] Figure 13 The spectrum of the unadsorbed cardamom extract after purification with macroporous resin ADS-17 is shown.

[0074] Figure 14 The spectrum of the unadsorbed cardamom extract after purification with macroporous resin D900 is shown.

[0075] Figure 15 The image shows a comparison of the spectra of unadsorbed cardamom extract after purification with eight different types of macroporous resins (including AB-8, D101, NKA-9, HPD-100, HPD-600, HP-20, ADS-17, and D900).

[0076] Figure 16 The spectrum of cardamom extract purified by desorption from AB-8 macroporous resin using 75% (v / v) ethanol aqueous solution is shown.

[0077] Figure 17 The spectrum of cardamom extract purified by desorption from D101 macroporous resin using 75% (v / v) ethanol aqueous solution is shown.

[0078] Figure 18 The spectrum of cardamom extract purified by desorption from NKA-9 macroporous resin using 75% (v / v) ethanol aqueous solution is shown.

[0079] Figure 19 The spectrum of cardamom extract purified by desorption from HPD-100 macroporous resin using 75% (v / v) ethanol aqueous solution is shown.

[0080] Figure 20 The spectrum of cardamom extract purified by desorption from HPD-600 macroporous resin using 75% (v / v) ethanol aqueous solution is shown.

[0081] Figure 21 The spectrum of cardamom extract purified by desorption from HP-20 macroporous resin using 75% (v / v) ethanol aqueous solution is shown.

[0082] Figure 22 The spectrum of cardamom extract purified by desorption from ADS-17 macroporous resin using 75% (v / v) ethanol aqueous solution is shown.

[0083] Figure 23 The spectrum of cardamom extract purified by desorption from D900 macroporous resin using 75% (v / v) ethanol aqueous solution is shown.

[0084] Figure 24 The image shows a comparison of the spectral scans of cardamom extract purified by desorption from eight different types of macroporous resins (including AB-8, D101, NKA-9, HPD-100, HPD-600, HP-20, ADS-17, and D900) using 75% (v / v) ethanol aqueous solution.

[0085] Figure 25 The UV absorption spectra of cardamom extract and chemical sunscreens (avobenzone and ethylhexyl methoxycinnamate) are shown in the diagram.

[0086] Figure 26 The image shows the results of detecting the sun protection effect of cardamom extract based on a UV test card. Detailed Implementation

[0087] Through extensive and in-depth research, the inventors unexpectedly discovered for the first time that extracting cardamom seeds from them using a specific concentration of ethanol / water mixed solvent (preferably 75±5% (v / v)) is safe and non-toxic. After purification with macroporous resin (preferably ADS-17), the resulting cardamom extract can simultaneously absorb wavelengths of 270nm-380nm, exhibiting excellent dual UV absorption properties (absorbing both UVA and UVB). This makes it highly suitable as an active ingredient in cosmetics, especially for preparing sunscreens. Based on this discovery, the inventors completed this invention.

[0088] the term Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains.

[0089] As used herein, the terms “comprising,” “including,” and “containing” are used interchangeably and include not only closed definitions but also semi-closed and open definitions. In other words, the terms include “consisting of” and “substantially consisting of”.

[0090] As used in this article, the terms "room temperature" or "normal temperature" refer to a temperature between 4 and 40 degrees Celsius. o C, preferably, 25±5 o C.

[0091] As used in this article, the terms "ethylhexyl methoxycinnamate" and "EHMC" are used interchangeably. They refer to a mainstream commercially available chemical sunscreen that primarily targets UVB absorption and protects against sunburn and sun damage in the center of the skin. The European Union Scientific Committee on Consumer Safety (SCCS) considers EHMC to have estrogenic and anti-androgenic activity and potential estrogen-interfering effects.

[0092] As used in this article, the terms "avobenzone" and "avobenzone" are used interchangeably. Avobenzone is a fat-soluble ingredient used in sunscreen products that absorbs all wavelengths of UVA (315-400nm) and is a derivative of dibenzoylmethane (DBM). Avobenzone has the highest absorption rate for UVA at a wavelength of 357nm. Some studies have shown that avobenzone has estrogen-like effects and may interfere with the endocrine system, thus having a certain impact on children and pregnant women, and limiting its use in certain populations.

[0093] As used in this article, the term "GP" refers to the standard for determining whether a sunscreen has broad-spectrum sun protection. Specifically, when the critical wavelength is <370nm, the sunscreen does not have broad-spectrum sun protection properties; when the critical wavelength is ≥370nm, it is determined to have weak broad-spectrum sun protection properties and is marked as "GP*"; when the critical wavelength is ≥375nm, it is determined to have medium broad-spectrum sun protection properties and is marked as "GP**"; and when the critical wavelength is ≥380nm, it is determined to have strong broad-spectrum sun protection properties and is marked as "GP***".

[0094] The cardamom of this invention In this invention, the terms "cardamom extract" and "cardamom extract of the present invention" are used interchangeably and refer to the cardamom extract obtained by the preparation method described in the first aspect of the present invention.

[0095] Cardamom is the nearly mature seed of *Alpinia katsumadai* Hayata, a plant in the Zingiberaceae family. It is a traditional Chinese medicine used for both food and medicine. It has a fragrant aroma, a pungent taste, and is warm in nature, entering the spleen and stomach meridians. It has the effects of drying dampness, promoting qi circulation, warming the middle jiao, and stopping vomiting. Clinically, it is used for symptoms such as abdominal distension and cold pain, nausea, vomiting, and diarrhea caused by cold-dampness obstruction and stagnation of spleen and stomach qi.

[0096] In this invention, the active ingredients of the cardamom extract include flavonoids, dibenzylheptanoids, volatile oils, etc. (such as galangin, cardamomin, pinocembrin, alderone, and myristic acid), and their structural formulas are shown below: In this invention, the cardamom extract is extracted with a specific concentration of ethanol / water mixed solvent (preferably 75±5 %v / v) and purified by macroporous resin (preferably ADS-17). The cardamom extract obtained by this invention, which is enriched to the maximum extent, can absorb the wavelength band of 270nm-380nm and has excellent performance in protecting against both UVA and UVB.

[0097] Preparation method of cardamom extract This invention provides a cardamom extract. The extract can be used as an active ingredient in sun protection (especially against UVA and UVB).

[0098] In a preferred embodiment of the present invention, the active ingredient is prepared by solvent extraction and / or column chromatography.

[0099] In this invention, the extraction can be performed once or multiple times (e.g., 2, 3, 4, or 5 times). After extraction, the extract is sequentially filtered and concentrated under reduced pressure to recover water (55℃~80℃, vacuum pressure 0.060~0.080Mpa) to obtain a crude extract. When multiple extractions are performed, the extracts from each extraction can be combined for subsequent processing.

[0100] For solvent extraction, there are no particular restrictions on the extraction solvent used. Representative examples include (but are not limited to): water, ethanol, methanol, and a mixture of one or more of the following solutions: water, ethanol, methanol, and water-ethanol mixtures. In this invention, for the solvent extraction method, an alcohol-water (mixed) solution of ethanol is used as the extraction solvent.

[0101] In this invention, the alcohol-water solution refers to a mixed solution formed by water and other solvents such as alcohols (especially C1-C4 alcohols, preferably ethanol). Typically, the ethanol content is 60-85% (v / v), preferably 75±5% (v / v), such as 75% (v / v), based on the total weight of the alcohol and water solvents.

[0102] For column chromatography, there are no particular limitations on column chromatography. Representative examples include (but are not limited to): activated carbon, silica gel, reversed-phase silica gel, macroporous resin, dextran gel, or a combination of several of these.

[0103] In this invention, macroporous resin is used for purification in column chromatography.

[0104] Specifically, the crude extract of the present invention is purified with twice the amount of ADS-17 macroporous resin, mixed with one volume of water, loaded onto the sample, washed with 4 BV of water, and then further eluented with ethanol solution to obtain ethanol eluent; the eluent containing cardamom extract (i.e., ethanol eluent) is collected.

[0105] In a preferred embodiment of the present invention, the method for preparing cardamom extract includes: using cardamom seeds as raw material (powder), extracting 1-5 times with an ethanol-water mixed solution (60-85% v / v ethanol solution, preferably 75±5% v / v ethanol solution) to obtain a crude extract (or concentrate) of cardamom extract.

[0106] Furthermore, in a preferred embodiment of the present invention, the crude extract can be further purified by a macroporous resin (preferably ADS-17) to obtain an eluent containing cardamom extract (i.e., an ethanol eluent), which is then prepared into an extract to obtain an extract with a higher content (or relative content) of cardamom extract, or an extract mainly containing cardamom extract.

[0107] The active ingredient of the present invention is in the form of powder or extract in daily chemical products. Specifically, the cardamom extract is vacuum dried or freeze-dried to obtain cardamom extract powder; or the extract is added to a solvent (such as water, 1,2-propanediol, 1,3-propanediol, butylene glycol, glycerol or 1,2-hexanediol, or a combination thereof) and dispersed to form cardamom extract (a transparent yellow liquid).

[0108] Compositions and their applications As used herein, the terms “cosmetic composition” and “composition of the present invention for broad-spectrum sun protection” are used interchangeably, including (a) the cardamom extract described herein; and (b) a pharmaceutically or cosmetically acceptable carrier.

[0109] Preferably, the composition is a cosmetic composition.

[0110] It is possible to prepare the cardamom extract of the present invention into a cosmetic composition, wherein the dosage form of the composition is a solid dosage form, a semi-solid dosage form, or a liquid dosage form, such as a solution, gel, cream, lotion, spray, ointment, paste, cake, powder, patch, etc.

[0111] Within the scope that does not impair the effects of the present invention, other ingredients commonly used in cosmetics may be added to the cosmetics of the present invention, such as film-forming agents, oil-soluble gelling agents, organically modified clay minerals, resins, moisturizers, preservatives, antibacterial agents, fragrances, salts, antioxidants, pH adjusters, chelating agents, cooling agents, anti-inflammatory agents, skin beautifying ingredients (whitening agents, cell activators, skin roughness improvers, blood circulation promoters, skin astringents, anti-seborrheic agents, etc.), vitamins, amino acids, nucleic acids, hormones, inclusion compounds, etc.

[0112] Oil-soluble gelling agents are selected from metal soaps such as aluminum stearate, magnesium stearate, and zinc myristate; amino acid derivatives such as N-lauroyl-L-glutamic acid and α,γ-di-n-butylamine; cyclodextrin fatty acid esters such as cyclodextrin palmitate, cyclodextrin stearate, and cyclodextrin 2-ethylhexanoate palmitate; sucrose fatty acid esters such as sucrose palmitate and sucrose stearate; benzylidene derivatives of sorbitol such as benzylidene sorbitol and dibenzylidene sorbitol; and organically modified clay minerals such as dimethylbenzyl dodecylammonium montmorillonite clay and dimethyl octadecylammonium montmorillonite clay. One, two, or more can be used as needed.

[0113] Moisturizers include: glycerin, sorbitol, propylene glycol, dipropylene glycol, 1,3-butanediol, glucose, xylitol, maltitol, polyethylene glycol, hyaluronic acid, chondroitin sulfate, pyrrolidone carboxylate, polyoxyethylene methyl glucoside, polyoxypropylene methyl glucoside, etc.

[0114] Antibacterial and preservative agents include: alkyl p-hydroxybenzoate, benzoic acid, sodium benzoate, sorbic acid, potassium sorbate, phenoxyethanol, etc. Antibacterial agents include: benzoic acid, salicylic acid, carbolic acid, sorbic acid, alkyl p-hydroxybenzoate, p-chloro-m-cresol, hexachlorophenol, benzalkonium chloride, chlorhexidine chloride, trichloro-N-carbonylaniline, triclosan, photosensitizer, phenoxyethanol, etc.

[0115] Antioxidants include: tocopherol, butylated hydroxyanisole, butylated hydroxytoluene, phytic acid, etc.; pH adjusters include: lactic acid, citric acid, glycolic acid, succinic acid, tartaric acid, dl-malic acid, potassium carbonate, sodium bicarbonate, ammonium bicarbonate, etc.; chelating agents include: alanine, sodium ethylenediaminetetraacetate, sodium polyphosphate, sodium metaphosphate, phosphoric acid, etc.; cooling agents include: L-menthol, camphor, etc.; anti-inflammatory agents include: allantoin, glycyrrhetinic acid, glycyrrhizic acid, tranexamic acid, azulene, etc.

[0116] Skin-beautifying ingredients include: whitening agents such as placental extract, arbutin, glutathione, and saxifrage extract; cell activators such as royal jelly, photosensitizers, cholesterol derivatives, and calf blood extract; skin roughness improvers; blood circulation promoters such as valeramide nonanoate, benzyl nicotinate, β-butoxyethyl nicotinate, capsaicin, gingerone, cantharides tincture, ichthammol, caffeine, tannic acid, α-borneol, nicotinic acid tocopherol, hexanicotinic acid inositol ester, cyclic mandelic acid ester, cinnarizine, tolazoline, acetylcholine, verapamil, strychnine, and γ-oryzanol; skin astringents such as zinc oxide and tannic acid; anti-seborrheic agents such as sulfur; and vitamins such as vitamin A oil, rosin oil, rosin acetate oil, and rosin palmitic acid oil; riboflavin, riboflavin butyrate, and flavin adenine nucleotide. Vitamins B2; Vitamins B6 such as pyridoxine hydrochloride, pyridoxine dicaprylate, and pyridoxine tripalmitate; Vitamins B12 and their derivatives; Vitamins B15 and their derivatives; Vitamins C such as L-ascorbic acid, L-ascorbic acid dipalmitate, L-ascorbic acid-2-sulfate sodium, and L-ascorbic acid phosphate dipotassium; Vitamins D such as ergocalciferol and cholecalciferol; Vitamins E such as α-tocopherol, β-tocopherol, γ-tocopherol, dl-α-tocopherol acetate, dl-α-tocopherol niacin, and dl-α-tocopherol succinate; Vitamin H; Vitamin P; Niacin, benzyl nicotinate, and nicotinamide; Pantothenic acid such as calcium pantothenate, D-panthenol, pantothenicotinyl ethyl ether, and acetylpantothenicotinyl ethyl ether; Biotin, etc.

[0117] Amino acids include glycine, valine, leucine, isoleucine, serine, threonine, phenylalanine, arginine, lysine, aspartic acid, glutamic acid, cystine, cysteine, methionine, tryptophan, etc. Nucleic acids include deoxyribonucleic acid, etc. Hormones include estradiol, vinyl estradiol, etc.

[0118] Preferred examples of cosmetics of the present invention include: skin care cosmetics, makeup cosmetics, and UV protection cosmetics. Examples include basic cosmetics such as lotions, creams, serums, sunscreens, mask ingredients, facial cleansers, and essences; and makeup cosmetics such as foundations, face powders, and blushes.

[0119] There are no particular restrictions on the form of the product; it can be liquid, emulsion, cream, solid, paste, gel, powder, multi-layered, mousse, spray, etc.

[0120] The present invention also provides a skin care method comprising the steps of: applying the cardamom extract or the composition described herein to an individual in need. Typically, the method is for the prevention and / or repair of UV-induced skin damage.

[0121] In another preferred embodiment, the effective concentration range of the cardamom extract is 1 μg / ml–500 mg / ml, such as 5 μg / ml, 10 μg / ml, 20 μg / ml, 50 μg / ml, 100 μg / ml or 200 μg / ml.

[0122] As used herein, the term "effective dose" means any amount of the cardamom extract or composition of the present invention, when used alone or in combination with another therapeutic agent or cosmetic, that promotes disease resolution, manifested as a reduction in the severity of disease symptoms, an increase in the frequency and duration of asymptomatic periods, or prevention of impairment or disability caused by the disease.

[0123] In this invention, the application to sunburn, sunburn (such as UV-induced erythema), tanning (such as hyperpigmentation), photoaging, phototoxic reactions and photosensitivity reactions, skin tumors, etc., includes both preventative and remedial applications. For example, for sunburn, this includes applying the cardamom extract or composition of this invention before, during, and / or after sun exposure to prevent and / or repair sunburn.

[0124] The main advantages of this invention include: (a) In the present invention, the ethanol / water mixed solvent of a specific concentration (preferably 75±5 %v / v) is used as the extraction solvent in the preparation process of the cardamom extract. The solvent is safe and non-toxic. After purification by macroporous resin (preferably ADS-17), the cardamom extract of the present invention can absorb the ultraviolet broad spectrum (absorbing the wavelength of 270nm-380nm) at the same time, and has excellent dual UV absorption performance of absorbing UVA and UVB.

[0125] (b) In this invention, the extraction method used for the cardamom extract is reflux extraction, which is simple, requires little equipment, and is inexpensive, making it suitable for industrial production.

[0126] (c) In this invention, the purification process uses macroporous resin ADS-17, and the resulting cardamom extract has excellent absorption effects for both UVA and UVB, with an increase of about 121% in UVB absorption intensity and about 70% in UVA absorption intensity, exhibiting excellent dual UV absorption performance.

[0127] (d) The cardamom extract of the present invention has a broad-spectrum ultraviolet absorption effect, which can prevent and / or repair skin damage induced by broad-spectrum ultraviolet rays, and is very suitable as an active ingredient in cosmetics, especially sunscreen products.

[0128] (e) The cardamom extract of the present invention is a plant-based ingredient with high safety and low irritation, and does not cause cell damage or affect cell viability.

[0129] (f) Compared with commercially available chemical sunscreens, the cardamom extract of the present invention has very few or no side effects, such as no estrogen interference, no interference with the endocrine system, and is suitable for a wide range of people.

[0130] The present invention will be further illustrated below with reference to specific embodiments. It should be understood that these embodiments are for illustrative purposes only and are not intended to limit the scope of the invention. Experimental methods in the following embodiments, unless otherwise specified, are generally performed under conventional conditions or as recommended by the manufacturer. Unless otherwise stated, percentages and parts are weight percentages and parts by weight.

[0131] Example 1: Extraction of cardamom extract using different solvents 1.1 Water extraction - Extraction of cardamom extract 10.08g of cardamom solid sample was pulverized using a pulverizer. The extractant (water) and cardamom sample were mixed evenly at a mass ratio of 1:9. The mixture was weighed to 471.61g (including container weight). The mixture was then refluxed in a water bath at 85℃ for 2 hours. After cooling, the mixture was weighed again. The weight was then replenished to 471.61g (including container weight) with the appropriate amount of extractant. The mixture was shaken well. The supernatant was diluted 100 times (equivalent to a 1000-fold dilution of the sample) and subjected to spectral scanning and absorption measurement using a UV spectrophotometer.

[0132] The extract sample was named Sample No. 1.1.

[0133] 1.2 Extraction of Cardamom Extract from 60% (v / v) ethanol aqueous solution 10.00g of cardamom solid sample was pulverized using a pulverizer. The extractant (60% (v / v) ethanol aqueous solution) and cardamom sample were mixed evenly at a mass ratio of 1:9. The mixture was weighed to 320.64g (including container weight). The mixture was then refluxed in a constant temperature water bath at 85℃ for 2 hours. After cooling, the mixture was weighed again. The weight was then made up to 320.64g (including container weight) with the appropriate amount of extractant. The mixture was shaken well. The supernatant was diluted 100 times (equivalent to a 1000-fold dilution of the sample) and then subjected to spectral scanning and absorption measurement using a UV spectrophotometer.

[0134] The extract sample was named Sample No. 1.2.

[0135] 1.3 Extraction of Cardamom Extract from 75% (v / v) Ethanol Aqueous Solution 10.07g of cardamom solid sample was pulverized using a pulverizer. The extractant (75% (v / v) ethanol aqueous solution) and cardamom sample were mixed evenly at a material-to-liquid mass ratio of 1:9. The mixture was weighed to 324.57g (including container weight). The mixture was then refluxed in a constant temperature water bath at 85℃ for 2 hours. After cooling, the mixture was weighed again. The weight was then made up to 324.57g (including container weight) with the appropriate amount of extractant. The mixture was shaken well. The supernatant was diluted 100 times (equivalent to a 1000-fold dilution of the sample) and then subjected to spectral scanning and absorption measurement using a UV spectrophotometer.

[0136] The extract sample was named Sample No. 1.3.

[0137] 1.4 Extraction of Cardamom Extract from 85% (v / v) Ethanol Aqueous Solution 10.06g of cardamom solid sample was pulverized using a pulverizer. The extractant (85% (v / v) ethanol aqueous solution) and cardamom sample were mixed evenly at a material-to-liquid mass ratio of 1:9. The mixture was weighed to 322.61g (including container weight). The mixture was then refluxed in a constant temperature water bath at 85℃ for 2 hours. After cooling, the mixture was weighed again. The weight was then made up to 322.61g (including container weight) with the appropriate amount of extractant. The mixture was shaken well. The supernatant was diluted 100 times (equivalent to a 1000-fold dilution of the sample) and then subjected to spectral scanning and absorption measurement using a UV spectrophotometer.

[0138] The extract sample was named Sample No. 1.4.

[0139] 1.5 Extraction of Cardamom Extract from Ionic Liquid Aqueous Solution 10.03g of cardamom solid sample was pulverized using a pulverizer. The pulverized cardamom was then added to a 50% (w / w) ionic liquid aqueous solution (the mass ratio of betaine, glycerol, and propylene glycol in the ionic liquid aqueous solution was 20:88:2) at a material-to-liquid mass ratio of 1:9 (50:50). The weight was measured to be 274.97g (including the container weight). The solution was then stirred at 25℃ for 60min, followed by centrifugation, filtration, collection of the filtrate, concentration, and drying to constant weight. The weight was measured again, and the appropriate extraction solvent was used to make up the weight to 274.97g (including the container weight). The solution was shaken well, and the supernatant was diluted 100 times (equivalent to a 1000-fold dilution of the sample). The solution was then subjected to spectral scanning and absorption measurement using a UV spectrophotometer.

[0140] The extract sample was named Sample No. 1.5.

[0141] 1.6 Experimental Results The cardamom extract obtained above and diluted 100 times was used to measure the absorbance at 290 nm and 350 nm using a UV spectrophotometer, as shown in Table 1. Figure 1-6 As shown.

[0142] The results showed that the cardamom solid sample extracted with 75% (v / v) ethanol aqueous solution exhibited the strongest absorbance at 290 nm and 350 nm. Compared with other concentrations of ethanol aqueous solution, the cardamom extract extracted with 75% (v / v) ethanol aqueous solution showed high absorption capacity for both UVB and UVA. Therefore, 75% (v / v) ethanol aqueous solution was selected as the optimal extraction solvent. Furthermore, compared with the absorbance extracted with water at 290 nm, the relative absorbance of the cardamom extract extracted with 75% (v / v) ethanol aqueous solution was 17.47; compared with the absorbance extracted with water at 350 nm, the relative absorbance of the cardamom extract extracted with 75% (v / v) ethanol aqueous solution was 27.62. This suggests that, relative to the increase in UVA absorption capacity, the cardamom extract of this invention shows a more significant increase in UVB absorption capacity (Table 1 and...). Figure 1-6 (As shown). *Relative value A: Relative value compared to absorbance extracted from water at 290 nm.

[0143] **Relative value B:** A relative value compared to the absorbance extracted from water at 350 nm.

[0144] Example 2: Purification of Cardamom Extract Using Different Types of Macroporous Resins 2.1 Pretreatment of macroporous resin In this embodiment, before purifying the cardamom extract with macroporous resin, various types of macroporous resins were soaked in a 95% ethanol aqueous solution for 24 hours, filtered, and then washed with deionized water until there was no ethanol odor.

[0145] 2.2 Preparation of crude extract of cardamom pods In this embodiment, the experimental method of Example 1.3 was used to prepare the crude extract of cardamom extract of the present invention (2000g raw material yielded approximately 200g crude extract).

[0146] 2.3 Purification of the crude extract of cardamom using a single type of macroporous resin Purification: The crude extract (or concentrate) of cardamom, corresponding to 10.0g of cardamom raw material, was diluted 10-fold with water and loaded onto 20.0g of each of the following pretreated and activated macroporous adsorption resins: weakly polar macroporous resin (AB-8), non-polar macroporous resin (D101, HPD-100, HP-20), polar macroporous resin (NKA-9, HPD-600, D900), and moderately polar macroporous resin (ADS-17). Each resin was eluted with 20mL of water. The eluent was collected, diluted 100-fold with water, and the spectra were scanned using a UV spectrophotometer. The absorbance of the eluent was measured at 290nm and 350nm. The adsorption rate was calculated. The results are shown in [Figure number missing]. Figure 7-15 Tables 2 and 3. After measuring the absorbance and calculating the adsorption rate, the water eluent was discarded.

[0147] The column was then elute with 60 mL of 75% (v / v) ethanol aqueous solution. The ethanol eluent was collected and diluted 100 times with 75% (v / v) ethanol aqueous solution. The absorbance of the eluent was measured at 290 nm and 350 nm using a UV spectrophotometer. The desorption rate was calculated, and the results are shown in [Figure number missing]. Figure 16-24 Tables 2 and 3.

[0148] The calculation formula is as follows: Adsorption rate = (Absorbance of active ingredient in the extract before column loading - Absorbance of active ingredient in the water eluent after loading) / Absorbance of active ingredient in the extract before column loading × 100% Resolution = Absorbance of active ingredient in ethanol solution eluent / (Absorbance of active ingredient in extract before column loading - Absorbance of active ingredient in water eluent after sample loading) Retention rate = Adsorption rate * Desorption rate 2.4 Experimental Results As shown in Table 2, Table 3 and Figure 7-24As shown, compared with purification using other macroporous resins, purification using AB-8, NKA-9, and ADS-17 significantly improved the retention rate of the cardamom extract at 290 nm. Specifically, compared with the average retention rate of the cardamom extract purified by each macroporous resin at 290 nm, the relative retention rate of the cardamom extract purified by AB-8 was 1.33, the relative retention rate of the cardamom extract purified by NKA-9 was 1.23, and the relative retention rate of the cardamom extract purified by ADS-17 was 1.7. Among them, the cardamom extract purified by ADS-17 had the highest relative retention rate at 290 nm.

[0149] Compared with purification using other macroporous resins, purification using AB-8, HP-20, and ADS-17 significantly improved the retention rate of the cardamom extract at 350 nm. Specifically, compared with the average retention rate at 350 nm of purification using various macroporous resins, the relative retention rate of the cardamom extract purified by AB-8 was 1.52, that of the cardamom extract purified by HP-20 was 1.62, and that of the cardamom extract purified by ADS-17 was 2.21. The cardamom extract purified by ADS-17 exhibited the highest relative retention rate at 350 nm (Tables 2 and 3). Figure 7-24 ).

[0150] Table 2. Data on purification using a single type of macroporous resin Table 3. Data analysis of purification using a single type of macroporous resin Surprisingly, compared to other types of macroporous resins, the cardamom extract purified using macroporous resin ADS-17 exhibited the highest adsorption rate, desorption rate, retention rate, relative retention rate at 350 nm, and relative retention rate at 290 nm compared to other types of macroporous resins.

[0151] Furthermore, based on the absorbance of the active ingredients in the crude extract of cardamom buds obtained in Table 1 and the retention rates in Table 3, the following formula Q4 was used: Absorbance of active ingredient in ethanol eluent ≈ retention rate * absorbance of active ingredient in crude extract of cardamom and nutmeg extract (Q4) The absorbance of the active substances in the ethanol eluent was calculated, and the results are shown in Table 4.

[0152] Table 4. Absorbance of Cardamom Extract in Ethanol Eluent As shown in Table 4, compared with purification using other macroporous resins, the absorbance of the cardamom extract at 290 nm was unexpectedly significantly increased when purified using AB-8, NKA-9, and ADS-17. The absorbance of the cardamom extract purified using AB-8 at 290 nm was approximately 1.42, the absorbance of the cardamom extract purified using NKA-9 at 290 nm was approximately 1.32, and the absorbance of the cardamom extract purified using ADS-17 was unexpectedly high at approximately 1.82 (approximately 3.55 times the absorbance when using D101 resin).

[0153] Compared to purification using other macroporous resins, purification using AB-8, HP-20, and ADS-17 unexpectedly significantly improved the absorbance of the cardamom extract at 350 nm. The absorbance of the cardamom extract purified using AB-8 at 350 nm was approximately 1.04, the absorbance of the cardamom extract purified using HP-20 at 350 nm was approximately 0.97, and the absorbance of the cardamom extract purified using ADS-17 was unexpectedly high at approximately 1.70 (approximately 4.59 times the absorbance when using D101 resin).

[0154] Compared to purification using other macroporous resins, purification using AB-8, NKA-9, and ADS-17 resulted in a higher sum of absorbance A1 for UVA and A2 for UVB for the obtained cardamom extract. totol The absorbance of the cardamom extract purified using AB-8 was also significantly improved, with the total absorbance of the cardamom extract purified using NKA-9 being approximately 2.47, the total absorbance of the cardamom extract purified using NKA-9 being approximately 2.25, and the total absorbance of the cardamom extract purified using ADS-17 being an even more unexpectedly high approximately 3.52.

[0155] This indicates that the absorbance A1 of cardamom extract for UVA is 1.20-2.0, preferably 1.25-1.85, and the absorbance A2 for UVB is 0.9-2.0, preferably 0.95-1.8.

[0156] Furthermore, the sum of the absorbance A1 for UVA and the absorbance A2 for UVB of the cardamom extract is A totol The range is 2.0-3.8, better is 2.2-3.6, even better is 2.4-3.6, and optimal is 3.3-3.6.

[0157] In addition, based on the absorbance in Table 1 and the retention rate in Table 3, the absorbance of the active ingredient in the ethanol eluent was calculated, that is, the absorbance of the cardamom extract obtained by extraction with 60% or 85% ethanol aqueous solution and then purification with ADS-17 at 290 nm and 350 nm (Table 5).

[0158] Table 5. Absorbance of cardamom extracts obtained by extraction with different ethanol / water mixed solvents and purification using ADS-17 As shown in Table 5, the absorbance (A1) of the cardamom extracts obtained by extraction with 60%–85% ethanol-water solution (ethanol / water mixed solvent) and purification with ADS-17 was unexpectedly significantly increased for UVA (approximately 1.46–1.82), and the absorbance (A2) for UVB was also unexpectedly significantly increased (approximately 1.35–1.70). The cardamom extract obtained by extraction with 75% ethanol-water solution and purification with ADS-17 exhibited the best dual UV absorption performance, with an absorbance (A1) of approximately 1.82 for UVA and an absorbance (A2) of approximately 1.7 for UVB.

[0159] In summary, the experimental results unexpectedly showed that, compared with other types of macroporous resins, the cardamom extract purified using macroporous resin ADS-17 had the highest absorbance for both UVA and UVB.

[0160] Therefore, the cardamom extract purified using ADS-17 exhibits excellent absorption effects for both UVA and UVB (Tables 2, 3, 4, and 5). Figure 7-24 Since macroporous resin ADS-17 has an excellent broad-spectrum absorption range, it is the preferred purification resin in subsequent embodiments.

[0161] Example 3: Broad-spectrum UV absorption test of the cardamom extract of the present invention and commercially available sunscreens. 3.1 Preparation of the cardamom extract of the present invention The ethanol eluent containing cardamom extract obtained in Examples 2-3-7 was concentrated to obtain cardamom extract (i.e. cardamom extract). Water, butanediol and 1,2-hexanediol were added to disperse the extract to prepare cardamom extract containing cardamom extract, wherein the mass concentration of cardamom extract in the cardamom extract was 5 (wt)%.

[0162] 3.2 Broad-spectrum ultraviolet absorption test In this embodiment, the UV-spectrum absorption effect of the cardamom extract of the present invention and commercially available sunscreens (avobenzone and ethylhexyl methoxycinnamate) was detected using a Purkinje UV spectrophotometer.

[0163] In this embodiment, the UV absorption spectra of cardamom extract, avobenzone, and ethylhexyl methoxycinnamate at the same concentration were measured using a Purkinje UV spectrophotometer, and the results are as follows: Figure 25 As shown in Table 6.

[0164] Avobenzone, a commercially available substance, was purchased from Shanghai Dibai Biotechnology Co., Ltd. (98 (wt)%, CAS: 70356-09-1). Ethylhexyl methoxycinnamate (98 wt), CAS: 5466-77-3, was purchased from Nanjing Dulai Biotechnology Co., Ltd.

[0165] 3.3 Experimental Results Figure 25 The results in Table 6 show that avobenzone has an absorption range of 303-380 nm, mainly absorbing the UVA band, but with almost no absorption of UVB; ethylhexyl methoxycinnamate has an absorption range of 270-330 nm, mainly absorbing the UVB band, but with almost no absorption of UVA; the cardamom extract has an absorption range of 270 nm-380 nm, showing effective absorption of both UVA and UVB; however, unexpectedly, compared with the single UV absorption peak of commercially available chemical sunscreens (such as avobenzone and ethylhexyl methoxycinnamate), the cardamom extract shows a double UV absorption peak, that is, absorption peaks appear at both 290.50 nm and 349.5 nm, indicating that the cardamom extract of the present invention has a strong absorption capacity for both UVA and UVB.

[0166] Furthermore, comparing the absorption peak widths, the cardamom extract was 1.43 times that of avobenzone and 1.83 times that of ethylhexyl methoxycinnamate. Figure 25 (and Table 6).

[0167] This indicates that, compared with mainstream commercially available chemical sunscreens, the cardamom extract of this invention has the advantage of broad-spectrum ultraviolet absorption.

[0168] Table 6: Comparison of UV absorption test results between cardamom extract and commercially available chemical sunscreens Example 4: Sunscreen efficacy test of the cardamom extract of the present invention and commercially available sunscreens. In this embodiment, the cardamom extract prepared in Example 3.1, the commercially available avobenzone and ethylhexyl methoxycinnamate from Example 3 were used to verify the sun protection effect.

[0169] 4.1 The sun protection effect of the cardamom extract of the present invention compared with commercially available sunscreens was tested based on the sun protection factor (SPF). 1) Experimental methods ① Testing instrument: SPF-290AS sun protection factor tester ②Testing environment: Environmental requirements: Temperature: 25.0℃±5℃; Humidity: 50%±10%.

[0170] ③ Testing process (1) Fix the PMMA plate onto the sample plate.

[0171] (2) First, determine the background curve of the blank plate, then use a spotter to evenly spot the sample onto the PMMA plate, and apply it with a finger wearing a latex finger cot at a concentration of 2 mg / cm². 2 Apply the sample gently and evenly to a 25 cm area. 2 A PMMA board of a certain size.

[0172] (3) Place the coated sample plate in a dark environment with a temperature of (25±5)℃ and a relative humidity of (50±10)% to air dry for 20 min.

[0173] (4) Turn on the power and preheat the instrument for 30 minutes. After confirming that it is normal, use the SPF-290AS analyzer to select 6 points on the PMMA plate for testing. The software will plot 6 light wavelengths and MPF for calculation and analysis to obtain the SPF value and standard deviation of one test, as well as the SPF value of each scanning point.

[0174] (5) Each sample should be coated and tested at least 3 times.

[0175] ④ Judgment criteria Sun Protection Factor (SPF) is a numerical value indicating a product's ability to protect against UVB rays. It's a protective indicator of how well sunscreens protect the skin from sunburn and sun damage. A higher SPF value means better protection against sunburn and sun damage, and it indicates that the sunscreen product can prolong the time before the skin is exposed to UVB rays. Generally, sunscreens with an SPF between 2 and 6 are considered low-level sunscreens, while those with an SPF between 6 and 8 are considered medium-level sunscreens.

[0176] Other testing standards for sun protection factor (SPF) are shown in Tables 7 and 8. Table 7 Table 8 PA is a standard for measuring the ability of sunscreen products to protect against UVA (long-wave ultraviolet radiation). UVA is a major cause of skin aging and tanning.

[0177] 2) Experimental Results The results are shown in Table 9. The cardamom extract of this invention has an SPF of 6.76, classifying it as a medium-grade sunscreen. Its UVA PFA value is 5.12, and its PA value is ++. The critical wavelength is 380.72nm ≥ 380nm, and its GP***. This indicates that the cardamom extract of this invention has a certain sun protection effect, which is outstanding among pure plant extract sunscreen products.

[0178] Table 9 4.2 The sun protection effects of the cardamom extract of this invention and commercially available sunscreens were determined using a UV test card. 1) Experimental methods ① Testing instruments: 365nm modeling UV lamp, UV test card ②Testing process (1) Place the 3-compartment culture dish on the UV test card.

[0179] (2) Add 5 ml of 2% cardamom extract, 2% avobenzone and water respectively. After irradiating with ultraviolet light for 10 seconds, observe and take pictures.

[0180] ③ Judgment criteria: Qualitatively judge the degree of absorption of ultraviolet light by observing the color depth of the ultraviolet test card.

[0181] 2) Experimental Results like Figure 26 As shown, the UV absorption effect of 2% of the cardamom extract of the present invention is between that of 2% avobenzone and water, exhibiting a moderate UV absorption effect.

[0182] All documents mentioned in this invention are incorporated herein by reference as if each document were individually incorporated by reference. Furthermore, it should be understood that after reading the foregoing teachings of this invention, those skilled in the art can make various alterations or modifications to this invention, and these equivalent forms also fall within the scope defined by the appended claims.

Claims

1. A method for preparing a broad-spectrum sunscreen extract of cardamom, characterized in that, The method includes the following steps: (S1) Extraction: The cardamom seed raw material was extracted with an ethanol / water mixed solvent to obtain an ethanol / water extract; (S2) Purification: The alcohol / water extract obtained in step (S1) is purified by macroporous resin. The purification process includes: loading the sample into the macroporous resin for resin adsorption, eluting with water, and then eluting with ethanol solution. The ethanol solution eluent is collected to obtain an eluent containing cardamom extract. (S3) The solution containing cardamom extract is concentrated to obtain cardamom extract; The macroporous resin is selected from the group consisting of ADS-17, AB-8, HP-20, or NKA-9, or a combination thereof.

2. The preparation method according to claim 1, characterized in that, In the ethanol / water mixed solvent, the volume concentration of ethanol is 50-90% (v / v), preferably 60-85% (v / v), and more preferably 65-80% (v / v).

3. The preparation method according to claim 1, characterized in that, The method includes the following steps: (S1) Extraction: Extract the cardamom seed raw material (powder) with ethanol / water mixed solvent, collect the filtrate and concentrate it to obtain cardamom extract crude extract or concentrated solution; (S2) Purification: Take the crude extract or concentrate obtained in step (S1), after optional dilution, load it onto a macroporous resin for resin adsorption, elute with water, and then elute with ethanol solution. Collect the ethanol solution eluent to obtain the eluent containing cardamom extract. (S3) The solution containing cardamom extract is concentrated to obtain cardamom extract.

4. A broad-spectrum sunscreen extract made from cardamom, characterized in that, The aforementioned cardamom extract is obtained by extracting cardamom seeds using an ethanol / water mixed solvent and then purifying them with macroporous resin. Furthermore, the cardamom extract described herein possesses dual UV absorption properties, absorbing both UVA and UVB.

5. The cardamom extract as described in claim 4, characterized in that, The cardamom extract has an absorbance A1 of 1.20-2.0 for UVA, preferably 1.25-1.85, and an absorbance A2 of 0.9-2.0 for UVB, preferably 0.95-1.

8.

6. The cardamom extract as described in claim 4, characterized in that, Compared with the absorbance A1w of the direct aqueous extract obtained by using water as a solvent for UVA, the ratio Z1 of the absorbance A1 to A1w of the cardamom extract for UVA is ≥10, preferably ≥12, and more preferably ≥15.

7. The cardamom extract as described in claim 4, characterized in that, Compared with the absorbance A2w of the direct aqueous extract obtained by using water as a solvent for UVB, the ratio Z1 of the absorbance A2 to A2w of the cardamom extract for UVB is ≥18, preferably ≥22, and more preferably ≥25.

8. The cardamom extract as described in claim 4, characterized in that, The cardamom extract is prepared using the method described in any one of claims 1-3.

9. The use of the cardamom extract as described in claim 4, characterized in that, The extract is used to prepare daily products or cosmetic products for protection against UVA and UVB.

10. A composition for protection against UVA and UVB, characterized in that, The composition comprises: (a) the cardamom extract as described in claim 4; and (b) A pharmaceutically or cosmetically acceptable carrier.

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