Preparation method of efficient biological fertilizer based on quantum tunneling effect regulation and control

Abstract

The invention discloses an efficient biological fertilizer preparation method based on quantum tunneling effect regulation and control, relates to the technical field of agricultural biology, in particular to an efficient biological fertilizer preparation method based on quantum tunneling effect regulation and control, and the method comprises the following steps: screening activated syntrophic bacteria and methanogens, and constructing a fermentation system in a customized anaerobic reactor. A nanometer microenvironment monitoring probe, a pressure regulation and control module and a temperature control device which are arranged in the reactor are used for monitoring and regulating and controlling the fermentation environment in real time, and the quantum tunneling efficiency of microbial electron transfer is accurately maintained in the optimal interval of 0.7-0.9. Feeding and output of materials are completed through a strain inoculation opening, a substrate feeding opening and a product discharging opening. The bio-fertilizer prepared by the method can significantly increase the crop yield, effectively reduce the bio-availability of soil heavy metals and degrade pesticide residues, and has the functions of efficient nutrient supply and soil environment remediation.

Description

Technical Field

[0001] This invention relates to the field of agricultural biotechnology, specifically to a method for preparing highly efficient bio-fertilizer based on quantum tunneling effect regulation. Background Technology

[0002] Traditional bio-fertilizer preparation mainly relies on the classical metabolic pathways of microorganisms. Its nutrient conversion efficiency is limited by the kinetic thresholds of conventional biochemical reactions, making it difficult to precisely match the nutrient release cycle with crop absorption needs, resulting in generally low fertilizer utilization efficiency. In environmental remediation areas such as soil heavy metal passivation and pesticide residue degradation, existing technologies mostly focus on the screening and domestication of functional strains, and have not yet systematically utilized the microscopic mechanisms of electron transfer between microorganisms to enhance the comprehensive effectiveness of bio-fertilizers.

[0003] Existing research has shown that in anaerobic environments, certain microorganisms (such as symbiotic bacteria and methanogens) can form electron transport channels through conductive pili, a process that may involve optimizing electron transport efficiency. However, there is currently no technical solution to simultaneously improve the nutrient supply efficiency and environmental remediation function of biofertilizers by directionally regulating this transport process, leaving significant room for improvement in the performance of biofertilizers in areas such as heavy metal passivation, pesticide degradation, and crop yield enhancement. Summary of the Invention

[0004] The purpose of this invention is to provide a highly efficient bio-fertilizer preparation method based on quantum tunneling effect regulation. By combining the reactor with the preparation method, the electron transfer process between microorganisms can be precisely controlled, significantly improving the nutrient conversion efficiency of the fermentation substrate. Simultaneously, the bio-fertilizer prepared by this method can effectively reduce the bioavailability of heavy metals in the soil and promote the degradation of pesticide residues, ultimately increasing crop yields and ensuring the safety and quality of agricultural products.

[0005] To achieve the above objectives, this invention employs the following technical solution: a highly efficient bio-fertilizer preparation method based on quantum tunneling effect regulation. The reaction chamber, as the core site of anaerobic fermentation, has a structural design directly related to the stability and efficiency of microbial metabolic activities. A nano-microenvironment monitoring probe is installed at a key location inside the chamber, continuously collecting physicochemical parameters of the microbial aggregation area, providing data support for the regulation of the quantum tunneling effect. A pressure regulation module achieves stepless adjustment of the internal pressure of the chamber through precision pneumatic elements, ensuring that the microbial quantum transmission channel maintains an ideal spatial configuration. The temperature control device adopts dual-loop temperature control technology, using temperature sensors distributed within the chamber to provide real-time data feedback and drive the heating or cooling units. The inoculation port and substrate inlet are located in different functional areas at the top of the chamber, employing a sealed quick-connect design to prevent gas leakage during operation. The product outlet is located below the side wall and equipped with a sampling valve for convenient mid-process detection; the control system interface uses a standardized communication protocol, enabling data synchronization with external monitoring equipment.

[0006] Furthermore, this monitoring probe is optimized based on the principles of atomic force microscopy, with its tip curvature radius controlled at the nanometer level, enabling precise detection of the quantum transport channel topology formed by microbial pili. The probe's built-in piezoelectric sensor detects changes in electron cloud density on the microbial membrane surface, converting the electrical signal into a tunneling efficiency value via Fourier transform. Monitoring data is updated every 5 seconds, and an alarm mechanism is automatically triggered when the coherence length falls below a critical value. The probe shell is made of biocompatible ceramic material to avoid toxic effects on the microbial community. Accuracy verification is performed using a standard nanogrid during calibration, ensuring that the linear error within the 1-100nm measurement range does not exceed ±0.5%. The probe cable employs a double-layer shielding structure to effectively suppress electromagnetic interference affecting weak quantum signals.

[0007] Furthermore, the module comprises a closed-loop control system consisting of a precision air pump, a buffer gas tank, and a digital pressure sensor. The air pump uses a stepper motor-driven piston structure, with each step pulse corresponding to a pressure change of 0.0001 MPa. The buffer gas tank volume is optimized through computational fluid dynamics to effectively smooth pressure pulsations. The digital pressure sensor is based on the silicon piezoresistive principle, and its Wheatstone bridge output signal is converted by a 24-bit ADC before being transmitted to the PLC controller. The control system employs a fuzzy PID algorithm to dynamically adjust the pressure setpoint based on real-time feedback of quantum tunneling efficiency. When an abnormality in quantum coherence parameters is detected, the system can adjust the pressure from the reference value to the target range within 30 seconds. All air connection points in the module use metal sealing rings to ensure no leakage occurs during continuous operation.

[0008] Furthermore, the device employs a dual-mode temperature control strategy: during normal operation, a jacketed water circulation system surrounding the cavity is used, with a proportional regulating valve controlling the mixing ratio of hot water and refrigerant; when rapid temperature adjustment is required, a built-in Peltier element is activated for auxiliary compensation. The temperature sensors are PT1000 platinum resistance thermometers with a measurement accuracy of 0.1%, distributed at three monitoring points (upper, middle, and lower). The control software establishes a three-dimensional temperature field model and calculates representative temperature values ​​using a weighted algorithm. The heating unit adopts a low power density design to avoid localized overheating that could affect microbial activity. The insulation layer uses a vacuum insulation panel structure, reducing the impact of ambient temperature fluctuations on the cavity to below 0.1℃ / h. Daily temperature calibration is performed, with cross-comparison using a standard thermometer at verification points.

[0009] Furthermore, in the specific implementation process, activation testing is required before inoculation to ensure that the OD600 values ​​of both symbiotic bacteria and methanogens reach the activity threshold of 0.8-1.0. Inoculation is performed in an anaerobic glove box, using a constant flow pump to control the inoculation rate to no more than 5 L / min. Nitrogen gas is injected simultaneously during substrate feeding to prevent aerobic microbial contamination. During the environmental parameter adjustment phase, the temperature control device slowly raises the temperature to the set value at a rate of 1℃ / min to avoid sudden temperature changes causing stress to the microbial community. Quantum tunneling efficiency monitoring uses a multi-point sampling and averaging method, recording a complete set of data every 10 minutes. When the efficiency value is below 0.7 for three consecutive cycles, the control system initiates a control program according to preset priorities: first adjusting pressure parameters, then supplementing electron mediators if the effect is not significant, and finally adjusting the inoculum ratio. Fermentation termination is determined by the volatile organic compound content dropping below 5 g / L and the quantum tunneling efficiency remaining stable within the target range for more than 12 hours.

[0010] Furthermore, the pressure increase operation employs a step-by-step adjustment strategy, increasing by 0.01 MPa each time and then stabilizing for 30 minutes to monitor the changing trends of quantum coherence parameters. Humic acid is sterilized using a 0.22 μm microporous membrane before addition and injected in a pulsed manner using a metering pump to ensure uniform diffusion within the chamber. The bacterial strain ratio is adjusted via a backup bacterial solution storage tank; the system automatically controls the start and stop times of the feed pump based on real-time calculated metabolic activity indices. When the symbiotic bacteria ratio reaches 1.2:1, the specific growth rate of methanogens is simultaneously monitored to prevent microbial imbalance leading to altered metabolic pathways. All control operations are recorded with timestamps and parameter change curves, creating a traceable process adjustment archive. If a single control method is ineffective, the system will activate a composite control mode, changing only one variable at a time to analyze causal relationships.

[0011] Furthermore, the temperature setpoint was determined based on the growth characteristic curve of the microbial strain. Methanogenic bacteria exhibited optimal activity at 36℃, while 38℃ was favorable for symbiotic bacteria to synthesize conductive fimbriae. The initial pressure value was referenced to the atmospheric pressure anaerobic fermentation baseline, allowing for operational flexibility in subsequent pressure adjustments. pH adjustment used a deoxygenated hydrochloric acid and sodium hydroxide solution, monitored in real-time by an online pH meter, with a control accuracy of ±0.05. During the start-up phase, the temperature was initially set to 35℃ and stabilized for 2 hours, gradually adjusting to the target value after the microbial community had adapted. The pressure parameter was slowly increased to 0.1 MPa after inoculation to avoid sudden pressure increases that could deform the fimbriae structure. pH adjustment was performed immediately after feeding to ensure that microorganisms began colonization at a suitable pH level. After all parameters stabilized, continuous monitoring for 4 hours was required to confirm that the fluctuation range met the requirements before proceeding to the formal fermentation stage.

[0012] Furthermore, this instrument is based on the principle of scanning tunneling microscopy, calculating the probability of electrons crossing the energy barrier by measuring the tunneling current intensity. The instrument's probe positioning accuracy reaches the sub-nanometer level, employing a constant height mode to scan the microbial membrane surface. The signal acquisition system uses lock-in amplification technology to extract weak quantum signals from background noise. The data processing unit incorporates an adaptive filtering algorithm to automatically compensate for baseline fluctuations caused by temperature drift. Calibration is performed using a standard quantum dot array, ensuring that the measurement error does not exceed ±0.02 within the 0.5-1.0 efficiency range. Measurement results are output every 2 seconds, and a repeat measurement program is automatically initiated for verification when an outlier is detected. The instrument undergoes periodic performance verification using standard samples to ensure that the response time remains within 10ms.

[0013] Furthermore, the plate and frame filter employs a multi-layer stainless steel filter plate structure. The first coarse filtration removes particles larger than 100μm, while the fine filtration stage uses a 0.45μm mixed cellulose ester membrane. The vacuum drying oven is equipped with a rotating rake arm to ensure uniform heating of the material during the heating process, and the drying endpoint is automatically determined by an online moisture meter. The granulator die holes are specially designed with an optimized length-to-diameter ratio of 8:1 to ensure consistent particle density. The ultraviolet sterilization device uses a surrounding lamp layout, with a light intensity sensor monitoring the irradiation intensity at each point in real time and automatically adjusting the lamp power to maintain the set value. Strict recording of parameters is maintained throughout the process: filtration pressure is controlled at 0.2-0.3MPa; moisture gradient is measured every 30 minutes during the drying process; the granulator speed is kept stable at 25r / min; and ultraviolet lamps must be replaced after 1000 hours of cumulative use.

[0014] Furthermore, the particle size distribution of the finished bio-fertilizer is tested using a laser particle size analyzer, requiring that at least 60% of the particles be 2-3mm. Particles exceeding this size must be returned to the granulation process for reprocessing. Moisture content is determined using an oven drying method, with sampling points covering the top, middle, and bottom layers of the packaging bag. Ten samples are randomly selected from each batch for parallel testing. The compressive strength of the particles is controlled within the range of 5-8 N / particle, ensuring it is not easily broken during transportation and facilitates rapid disintegration after application to the soil. Double-layer aluminum-plastic composite film is used for packaging, with a desiccant added to the inner layer to maintain a relative humidity below 45% inside the bag. Samples of each batch are retained for six months, and nutrient content changes are regularly monitored to establish a product quality traceability system. A germination index test is recommended before application to ensure no physiological toxicity to crop seedlings.

[0015] This invention provides a highly efficient bio-fertilizer preparation method based on quantum tunneling effect regulation, which has the following beneficial effects:

[0016] 1. Significantly improves microbial metabolic efficiency and nutrient conversion rate

[0017] This invention fundamentally optimizes the electron transport pathway between symbiotic bacteria and methanogens by precisely controlling the quantum tunneling efficiency in the anaerobic fermentation system to the optimal range of 0.7-0.9. At the nanoscale, the quantum tunneling effect allows electrons to overcome the energy barriers of classical biochemical reactions, directly accelerating the metabolic coupling process between the intracellular and extracellular spaces of microorganisms. This mechanism significantly improves the decomposition rate of organic matter in the fermentation substrate and the synthesis efficiency of readily available nutrients (such as ammonium nitrogen, readily available phosphorus, and readily available potassium). Specifically, compared to fermentation processes relying on traditional natural metabolic pathways, the nutrient conversion efficiency guided by this invention is increased by 25-35%. This not only ensures the rapid release of fertilizer nutrients but, more importantly, achieves a high degree of matching between the nutrient release cycle and the growth and absorption rhythm of crops, thereby significantly improving fertilizer utilization and reducing nutrient loss and waste in agricultural production.

[0018] 2. Targeted enhancement of soil's heavy metal passivation capacity to ensure agricultural product safety.

[0019] During the fermentation process, the bio-fertilizer prepared by this invention exhibits significantly enhanced microbial metabolic activity due to optimized regulation of the quantum tunneling effect. The resulting extracellular polymers and specific enzyme systems effectively alter the chemical forms of heavy metals in the soil. Specifically, this bio-fertilizer can transform exchangeable heavy metals such as lead and cadmium, which are highly bioavailable and easily absorbed by crops, into stable, less mobile residual forms. Field trial data confirms that the content of exchangeable lead and cadmium in the soil can be reduced by 45-60% after application. This targeted passivation effect blocks the risk of heavy metal transmission through the food chain at its source, resulting in lead and cadmium content in the final agricultural products being far below the stringent requirements of GB 2762-2022 "National Food Safety Standard: Limits of Contaminants in Food." This provides a reliable technical guarantee for producing safe and high-quality agricultural products from heavy metal-contaminated farmland, directly contributing to the improvement of food safety levels.

[0020] 3. Highly efficient at degrading organic pollutants and improving the soil ecological environment.

[0021] Based on quantum tunneling-regulated microbial metabolic processes, the activity of key extracellular activating enzymes (such as peroxidase and laccase) can be significantly enhanced by 40-60%. These highly active enzyme systems can specifically recognize and efficiently break key chemical bonds such as PO bonds in organophosphorus pesticide molecules, thereby achieving deep degradation of pesticide residues. Practical applications show that the bio-fertilizer of this invention can achieve a degradation rate of 35-50% for common organophosphorus pesticides such as chlorpyrifos in soil. This function not only directly eliminates pesticide residue pollution in the soil and reduces its inhibitory effect on soil microbial communities and enzyme systems, but also helps restore the health of the soil, creating a cleaner and more favorable growth environment for crop roots, demonstrating its outstanding value in the control of agricultural non-point source pollution.

[0022] 4. Achieve coordinated growth in crop yield and quality.

[0023] This invention optimizes the entire fermentation process of bio-fertilizer through quantum-level control. The resulting product not only efficiently supplies nutrients, but its contained active microorganisms and metabolites also stimulate crop root development and enhance crop resistance. Field trials using wheat as the subject showed that the yield of wheat treated with this invention increased by 15-22% compared to the control group treated with traditional bio-fertilizer. More importantly, while significantly increasing yield, the heavy metal content in crop grains was significantly reduced, and the quality was improved. This indicates that this invention successfully overcomes the potential contradiction between "high yield" and "high quality" in traditional agricultural management, achieving a synergistic improvement in crop yield and food safety on polluted soils. This has dual positive significance for ensuring both the quantity and quality of food supply.

[0024] 5. Enhance the stability and sustainability of soil micro-ecosystems

[0025] The application of this bio-fertilizer has a profound and positive impact on the structure of the soil microbial community. High-throughput sequencing analysis showed that the Shannon diversity index of soil microorganisms increased by more than 30% after application. This indicates that the soil microbial species are more abundant, more evenly distributed, and the ecosystem is more stable. A healthy and diverse soil microbial community is the core of soil fertility, enhancing the decomposition of organic matter, nutrient cycling, and structural maintenance capabilities of the soil. Therefore, this invention not only provides seasonal nutrients and restoration functions, but also lays a solid foundation for the self-sustaining of soil fertility and the sustainable development of agricultural production by cultivating and optimizing the soil micro-ecosystem, with long-term and cumulative benefits. Attached Figure Description

[0026] To more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings in the following description are merely exemplary, and those skilled in the art can derive other embodiments based on the provided drawings without creative effort.

[0027] Figure 1 This is a schematic diagram of the overall structure of the present invention;

[0028] Figure 2 This is a flowchart illustrating the quantum tunneling effect modulation process of the present invention.

[0029] Figure 3 This is a bar chart comparing the distribution of heavy metal (lead, cadmium) speciation in the soil between the experimental group and the control group of this invention.

[0030] Figure 4 This is a bar chart comparing wheat yield and grain heavy metal (lead, cadmium) content between the experimental group and the control group of this invention.

[0031] Figure 5 This is a graph showing the dynamic changes in quantum tunneling efficiency and extracellular activating enzyme (peroxidase, laccase) activity during the fermentation process of this invention. Detailed Implementation

[0032] Exemplary embodiments will now be described in detail, examples of which are illustrated in the accompanying drawings. When the following description relates to the drawings, unless otherwise indicated, the same numerals in different drawings denote the same or similar elements. The embodiments described in the following exemplary embodiments do not represent all embodiments consistent with this disclosure. Rather, they are merely examples of apparatuses consistent with some aspects of this disclosure as detailed in the appended claims.

[0033] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are within the scope of protection of the present invention.

[0034] Example 1: Materials and equipment for preparing bio-fertilizer under standard conditions:

[0035] The fermentation process used symbiotic bacteria with preservation numbers ATCC 49887 and methanogenic bacteria with preservation numbers ATCC 29546; the fermentation substrate consisted of corn stalks and chicken manure mixed and decomposed at a mass ratio of 3:2; a 50L custom anaerobic reactor, a QT-2000 quantum coherence analyzer, an ETE-300 electron tunneling efficiency analyzer, and an HPCS-50 high-precision constant temperature and pressure control system were used.

[0036] Steps and parameters:

[0037] 1. Activation of bacterial strains: The two bacterial strains were inoculated into enhanced medium (5 g / L tryptone, 3 g / L yeast extract, 2 g / L sodium acetate, 1 g / L sodium bicarbonate, 10 mL / L trace element solution, pH 7.5) and cultured under anaerobic conditions at 37°C for 48 hours until the OD600 value reached 0.9.

[0038] 2. Fermentation Start-up: Add 30L of fermentation substrate to the reactor, and inoculate with 5L of symbiotic bacteria culture and 5L of methanogenic bacteria culture (volume ratio 1:1). Adjust the pH of the system to 7.5, stabilize the temperature at 37℃, and set the initial pressure to 0.1MPa.

[0039] 3. Process control: After 12 hours of fermentation, the quantum tunneling efficiency was measured to be 0.62. Control measures were then implemented: the reaction pressure was increased to 0.13 MPa, 0.8 g / L of humic acid with a purity ≥95% was added, and the volume ratio of symbiotic bacteria to methanogens was adjusted to 1.2:1 (achieved by adding the appropriate bacterial solution).

[0040] 4. Monitoring and Termination: Continuous monitoring showed that the quantum tunneling efficiency increased to 0.78 after 24 hours of fermentation, and the extracellular activating enzyme activity was 120 U / mL; after 72 hours of fermentation, the volatile organic compound content of the system decreased to 4.2 g / L, and fermentation was stopped.

[0041] 5. Product preparation: The fermentation product was filtered through a plate and frame filter (0.45 μm pore size), vacuum dried at 55℃ / -0.09 MPa for 24 hours, granulated by disc granulation, and sterilized by ultraviolet light (wavelength 254 nm, intensity 300 μW / cm²). 2 After 30 minutes, a bio-fertilizer product with a particle size of 2-4 mm and a moisture content of 10.5% is obtained.

[0042] Example 2: Application materials and equipment for different fermentation substrates:

[0043] The inoculum and equipment are the same as in Example 1. The fermentation substrate is changed to a mixture of pig manure and wheat straw in a mass ratio of 2:3, and the C / N ratio is adjusted to 25, and the moisture content is adjusted to 60%.

[0044] Steps and parameters:

[0045] 1. The activation steps for the bacterial strain are the same as in Example 1.

[0046] 2. Fermentation start-up: After the substrate is added to the reactor, inoculate with bacterial culture (initial volume ratio 1:1), adjust the pH to 7.2, the temperature to 36℃, and the initial pressure to 0.1MPa.

[0047] 3. Process control: After 16 hours of fermentation, the quantum tunneling efficiency was 0.65. Control measures were implemented: the pressure was increased to 0.12 MPa, 0.5 g / L of humic acid was added, and the inoculum volume ratio was adjusted to 1.15:1.

[0048] 4. Monitoring and Termination: After 80 hours of fermentation, the quantum tunneling efficiency stabilized at 0.75 and the volatile organic compound content decreased to 4.8 g / L, at which point fermentation was stopped.

[0049] 5. Finished product preparation: The subsequent processing technology is the same as in Example 1, and the resulting bio-fertilizer has a moisture content of 11.2%.

[0050] Example 3: Materials and devices for precise control of quantum tunneling efficiency:

[0051] Same as Example 1.

[0052] Steps and parameters:

[0053] 1. The steps for strain activation and fermentation start-up are the same as in Example 1.

[0054] 2. Process control: During fermentation, when the quantum tunneling efficiency first drops below 0.7 (monitored value is 0.68), the pressure is preferentially adjusted from 0.1 MPa to 0.11 MPa. After 4 hours of monitoring, the efficiency rises to 0.71, but does not reach above 0.75, so 0.7 g / L humic acid is added.

[0055] 3. Monitoring and Termination: Eight hours after adding humic acid, the efficiency stabilized at 0.79. Fermentation was terminated after 96 hours when the volatile organic compound content reached the target.

[0056] 4. Finished product preparation: The subsequent processing technology is the same as in Example 1, and the resulting bio-fertilizer has a particle size uniformity of ≥90%.

[0057] Example 4: Materials used to verify the effectiveness of remediation of heavy metal contaminated soil:

[0058] The bio-fertilizer product prepared using Example 1.

[0059] Experimental Design: Wheat was planted in lead- and cadmium-contaminated farmland. An experimental group (applied with 200 kg / mu of the bio-fertilizer of this invention) and a control group (applied with an equal amount of commercially available traditional bio-fertilizer) were set up. Each plot was 20 m². 2 Repeated 3 times.

[0060] Results: Post-harvest analysis showed that the exchangeable lead content in the soil of the experimental group was 12.3 mg / kg, a 52% decrease compared to the control group (25.6 mg / kg); the exchangeable cadmium content was 0.8 mg / kg, a 48% decrease compared to the control group (1.5 mg / kg). The degradation rate of chlorpyrifos in the soil of the experimental group was 42%, while that of the control group was 18%. All differences were statistically significant (P<0.05).

[0061] Example 5: Materials used to verify the effects of increased crop yield and improved quality:

[0062] The bio-fertilizer product prepared using Example 1.

[0063] Experimental design: Same as the field trial design in Example 4.

[0064] Results: The wheat yield in the experimental group was 580 kg / mu, compared to 492 kg / mu in control group 1 and 430 kg / mu in control group 2 (without bio-fertilizer). The yield in the experimental group was 18% higher than that in control group 1. The lead content and cadmium content in the wheat grains of the experimental group were 0.02 mg / kg and 0.008 mg / kg, respectively, meeting the GB 2762-2022 standard and significantly lower than those in control group 1 (lead 0.035 mg / kg, cadmium 0.015 mg / kg). The soil peroxidase and laccase activities, as well as the Shannon index of the microbial community, were significantly higher in the experimental group than in control group 1.

[0065] All electrical components mentioned in this article are connected to an external main controller and 220V AC mains power. The main controller can be a conventional known device such as a computer for control. The detailed description of known functions and components is omitted in the specific implementation of this disclosure. To ensure the compatibility of the device, the operating methods used are consistent with the parameters of commercially available instruments.

[0066] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A method for preparing highly efficient bio-fertilizer based on quantum tunneling effect regulation, comprising a reaction chamber (1), characterized in that: The inner end of the reaction chamber (1) is provided with a nano-microenvironment monitoring probe (2), the inner end of the reaction chamber (1) is provided with a pressure regulation module (3), and the inner end of the reaction chamber (1) is provided with a temperature control device (4). The upper end of the reaction chamber (1) is provided with a bacterial inoculation port (5), the upper end of the reaction chamber (1) is provided with a substrate inlet (6), the side end of the reaction chamber (1) is provided with a product outlet (7), and the side end of the reaction chamber (1) is provided with a control system interface (8).

2. The method for preparing high-efficiency bio-fertilizer based on quantum tunneling effect regulation according to claim 1, characterized in that: The nano-microenvironment monitoring probe (2) has a monitoring accuracy of 1 nm and is used to monitor the nano-microenvironment parameters of electron transfer between microorganisms in the reaction chamber (1) in real time.

3. The method for preparing a highly efficient bio-fertilizer based on quantum tunneling effect regulation according to claim 1, characterized in that: The pressure control module (3) can precisely adjust the pressure in the reaction chamber (1) within the range of 0.1MPa to 0.15MPa, and the pressure fluctuation is controlled to be no greater than ±0.005MPa.

4. The method for preparing high-efficiency bio-fertilizer based on quantum tunneling effect regulation according to claim 1, characterized in that: The temperature control device (4) can stabilize the temperature in the reaction chamber (1) within the range of 36°C to 38°C, and control the temperature fluctuation within ±0.5°C.

5. A method for preparing high-efficiency bio-fertilizer based on quantum tunneling effect regulation according to any one of claims 1-4, characterized in that, Includes the following steps: S1: The activated symbiotic bacteria and methanogenic bacteria are introduced into the reaction chamber (1) through the inoculation port (5), and the fermentation substrate is added through the substrate feed port (6); S2: The internal environment of the reaction chamber (1) is adjusted to the preset conditions by the temperature control device (4) and the pressure regulation module (3); S3: Real-time monitoring of quantum tunneling efficiency using a nano-microenvironment monitoring probe (2); S4: When the quantum tunneling efficiency is lower than 0.7, the quantum tunneling efficiency can be dynamically controlled within the range of 0.7 to 0.9 by adjusting the pressure control module (3) to increase the pressure, adding the electron mediator humic acid, or adjusting the ratio of bacterial activity replenishment. S5: After fermentation is completed, the fermentation product is discharged through the product outlet (7) and then processed to obtain the finished bio-fertilizer.

6. The method for preparing a high-efficiency bio-fertilizer based on quantum tunneling effect regulation according to claim 5, characterized in that: In step S4, the pressure regulation module (3) increases the pressure by increasing the reaction pressure from 0.1 MPa to 0.12-0.15 MPa; the addition of humic acid, an electron mediator, is to supplement humic acid with a concentration of 0.5-1.0 g / L and a purity of ≥95%; the adjustment of the bacterial activity replenishment ratio is to dynamically adjust the mixed volume ratio of symbiotic bacteria and methanogenic bacteria from 1:1 to 1.2:

1.

7. The method for preparing a high-efficiency bio-fertilizer based on quantum tunneling effect regulation according to claim 5, characterized in that: In step S2, the preset conditions are: temperature 36-38℃, initial pressure 0.1MPa, and pH value 7.2-7.

8.

8. The method for preparing high-efficiency bio-fertilizer based on quantum tunneling effect regulation according to claim 5, characterized in that: In step S3, the quantum tunneling efficiency is measured by an electronic tunneling efficiency measuring instrument connected to the control system interface (8), and the response time of the measuring instrument is no more than 10ms.

9. The method for preparing a high-efficiency bio-fertilizer based on quantum tunneling effect regulation according to claim 5, characterized in that: In step S5, the subsequent processing includes plate and frame filtration, vacuum drying, granulation, and ultraviolet sterilization; the vacuum drying conditions are 55°C and a vacuum degree of -0.09 MPa; the ultraviolet sterilization conditions are a wavelength of 254 nm and an irradiation intensity of 300 μW / cm². 2 Irradiation time: 30 minutes.

10. A method for preparing high-efficiency bio-fertilizer based on quantum tunneling effect regulation according to any one of claims 5-9, characterized in that: The bio-fertilizer has a particle size of 2-4 mm and a moisture content of no more than 12%.

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