Traditional Chinese medicine preparation for tonifying qi, benefiting blood and improving immunity and preparation method of traditional Chinese medicine preparation

Abstract

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine preparation for tonifying qi, benefiting blood and improving immunity. The traditional Chinese medicine preparation for tonifying qi, benefiting blood and improving immunity is prepared from the following raw materials in parts by weight: 12 to 17 parts of fructus rosae laevigatae, 5 to 10 parts of black bamboo root, 8 to 12 parts of herba dendrobii, 8 to 12 parts of fructus tsaoko, 7 to 10 parts of radix angelicae sinensis, 6 to 9 parts of radix rehmanniae praeparata, 5 to 10 parts of elm leaf, 3 to 7 parts of poria cocos, 5 to 10 parts of radix polygonati officinalis and 1 to 4 parts of liquorice root. The traditional Chinese medicine preparation has the effects of tonifying qi, lifting yang, nourishing essence and blood, promoting the secretion of saliva or body fluid and nourishing blood, is stable in curative effect, has the effects of tonifying qi and nourishing blood, and can enhance the immunity of the organism and improve the health of the organism.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of traditional Chinese medicine, and particularly relates to a traditional Chinese medicine preparation for tonifying qi and blood and improving immunity and a preparation method thereof. BACKGROUND

[0002] Qi and blood are the foundation of life activities. It is stated in Huangdi Neijing that "qi and blood disorders will cause all diseases". Qi and blood deficiency is a common syndrome in clinical practice of traditional Chinese medicine, and the core manifestations are fatigue, pale complexion, palpitation and shortness of breath, dizziness, low immunity and the like. With the acceleration of modern life rhythm, the increase of mental stress, environmental pressure and changes in lifestyle, the population with qi and blood deficiency has significantly expanded. The direct consequence of this trend is the problem of low immunity, such as repeated colds, chronic fatigue syndrome and the like, which seriously affects the health and quality of life of the people.

[0003] The immune system of the human body is a precise defense system for recognizing "self" and "non-self", resisting invasion of foreign pathogens and maintaining stability of the internal environment. The decline of immunity indicates that the body has entered a sub-health state, and improving the function of the immune system is a key way to enhance resistance, resist diseases and improve sub-health. Although chemically synthesized immune enhancers have rapid effects, their side effects, potential dependence and drug resistance problems caused by long-term use constitute significant limitations. Therefore, exploring safe, effective and suitable long-term application of immune regulation strategies has become a front focus and urgent need in the field of life science and drug research and development.

[0004] Traditional Chinese medicine has a profound theoretical basis and practical experience in improving immunity. Huangdi Neijing proposes "the correct qi is stored in the body, and the evil cannot be attacked", which elaborates the core immune thought of "supporting the correct qi and consolidating the foundation", and emphasizes that the barrier against external evils is built by tonifying the correct qi and qi and blood of the human body. This provides an important guidance for regulating immunity by using traditional Chinese medicine. However, the current mainstream intervention means of traditional Chinese medicine, such as traditional decoction, generally has problems such as large dosage, poor patient compliance and low bioavailability of effective components, resulting in poor curative effect. Therefore, there is an urgent need for a traditional Chinese medicine preparation with innovative compatibility relationship and synergistic effect to enhance the immunity of the body. SUMMARY

[0005] The first object of the application is to provide a traditional Chinese medicine preparation for tonifying qi and blood and improving immunity. The traditional Chinese medicine preparation of the application focuses on the compatibility relationship of monarch, minister, assistant and guide, and can tonify qi and blood and improve immunity.

[0006] The second object of the application is to provide a preparation method of the traditional Chinese medicine preparation for tonifying qi and blood and improving immunity. The preparation method is simple and easy to operate.

[0007] The object of the application is achieved by the following technical solutions: The application discloses a Chinese medicine preparation for tonifying qi and blood and improving immunity, which comprises the following raw materials in parts by weight: 12-17 parts of chamaepermum lanceolatum, 5-10 parts of purple bamboo root, 8-12 parts of dendrobium candidum, 8-12 parts of amomum villosum, 7-10 parts of angelica sinensis, 6-9 parts of prepared rehmannia root, 5-10 parts of Ulmus pumila, 3-7 parts of poria cocos, 5-10 parts of polygonatum, and 1-4 parts of licorice.

[0008] Further, the Chinese medicine preparation for tonifying qi and blood and improving immunity comprises the following raw materials in parts by weight: 15 parts of chamaepermum lanceolatum, 8 parts of purple bamboo root, 10 parts of dendrobium candidum, 10 parts of amomum villosum, 8 parts of angelica sinensis, 7 parts of prepared rehmannia root, 9 parts of Ulmus pumila, 5 parts of poria cocos, 8 parts of polygonatum, and 2 parts of licorice.

[0009] The application further provides a preparation method of the Chinese medicine preparation for tonifying qi and blood and improving immunity. (1) according to the weight parts, taking each raw material medicine, chamaepermum lanceolatum, purple bamboo root, dendrobium candidum, amomum villosum, angelica sinensis, prepared rehmannia root, Ulmus pumila, poria cocos, polygonatum, and licorice are soaked and decocted with water, filtered to obtain filtrate A and residue A; (2) the residue A after filtering in step (1) is again decocted with water, filtered to collect filtrate B and residue B; (3) the filtrate A and the filtrate B are combined, concentrated into extract, and the extract is prepared into a corresponding dosage form with a pharmaceutically acceptable excipient to obtain the product.

[0010] Further, in step (1), the total mass of the raw medicine and the solid-liquid ratio of water are 1g: (8-12) mL.

[0011] Further, in step (1), the soaking time is 20-30 min.

[0012] Further, in step (2), the solid-liquid ratio of the residue A and water is 1g: (5-10) mL.

[0013] Further, the decocting time in step (1) is 3-5h, and the decocting time in step (2) is 1-3h.

[0014] Further, the relative density of the extract in step (3) is 1.0-1.2.

[0015] Further, the dosage form is a tablet.

[0016] To achieve the above object, the technical scheme adopted by the application is as follows: Cherokee rose: the dried mature fruit of Rosa laevigata Michx. of Rosaceae, with sour, sweet and astringent taste, and neutral nature. It belongs to kidney, bladder and large intestine channels. It has the functions of consolidating essence, reducing urine, arresting vaginal bleeding, astringing intestines and stopping diarrhea. It is used to treat spermatorrhea, enuresis, frequent micturition, metrorrhagia, leukorrhagia, chronic diarrhea and dysentery. Bencaojingshu records that Shijidai says that astringency can remove leakage. Spleen deficiency causes diarrhea and incontinence, and no astringent can cure it. Bladder deficiency causes incontinence, and kidney and bladder are interrelated. Kidney deficiency causes spermatorrhea, and this medicine (Cherokee rose) has warm and astringent properties, and can enter three channels to converge and astringe, so it can treat various diseases.

[0017] Purple bamboo root: the rhizome of Phyllostachys nigra of Poaceae. It can be harvested throughout the year. It is pungent and bland, and cool in nature. It belongs to the liver channel. It has the effects of expelling wind and dampness, promoting blood circulation and detoxifying. It is used to treat rheumatic arthralgia, contusion and injury, and rabies bite. Chongqing Herbal records that it can move qi, break accumulation, clear liver channel wind-heat, treat qi and blood stagnation, mass, and amenorrhea.

[0018] Golden stone orchid: the stem of Dendrobium nobile of Orchidaceae. It is sweet and cold, and belongs to the stomach and kidney channels. It has the effects of nourishing yin and clearing heat, benefiting the stomach and generating saliva, and strengthening the waist. It is used to treat heat disease, dry mouth and thirst, post-illness heat, and dim eyesight. Yapo Shilun records that it benefits qi and removes heat. It is used to treat male weakness of the waist and legs, invigorating yang, expelling skin wind, treating bone cold, deficiency, and invigorating the kidney and accumulating essence, and treating lumbago.

[0019] Passiflora edulis: Passiflora edulis is a woody evergreen liana. The root can be used as medicine to treat contusion and injury. The fruit can be used to make wine, fruit pulp and beverage, and the oil can be used for food. The fruit and seed can be used to treat hernia, uterine prolapse, rheumatic bone pain, etc.

[0020] Angelica: the root of Angelica sinensis of Umbelliferae, sweet and pungent, warm, and belongs to the liver, heart and spleen channels. It has the effects of tonifying blood and regulating menstruation, promoting blood circulation and relieving pain, and moistening the intestines and relieving constipation. It is used to treat blood deficiency, dizziness, palpitation, amenorrhea, dysmenorrhea, cold abdominal pain, dry constipation, rheumatic arthralgia, contusion and injury, and carbuncle and sore. Bie Lu records that it can warm the center and relieve pain, expel internal blood stasis, treat wind convulsion, sweat, dampness, and cold, invigorate five zang organs, and promote muscle growth.

[0021] Rehmannia root: the tuber of Rehmannia glutinosa of Scrophulariaceae, steamed. It is sweet and slightly warm, and belongs to the liver and kidney channels. It has the effects of tonifying liver and kidney, nourishing the heart and calming the mind, and clearing heat and detoxifying. It is used to treat yin deficiency and blood deficiency, weakness of the waist and knees, labor cough and bone steaming, spermatorrhea, metrorrhagia, irregular menstruation, diabetes, frequent urination, deafness, and dim eyesight. Gangmu records that it can fill bone marrow, grow muscle, produce essence and blood, tonify five zang organs, treat internal deficiency, regulate blood vessels, benefit the ears, darken hair, and treat five kinds of yang deficiency in men and irregular menstruation, metrorrhagia, and irregular menstruation in women.

[0022] Ulmus pumila L. The leaf of Ulmus pumila L. is sweet in taste and flat in nature. It is associated with the spleen and stomach meridians. It has the effects of clearing heat and promoting diuresis, soothing the nerves, expelling phlegm and relieving cough. It is used for edema, difficult urination, urinary stones, urine turbidity, insomnia, summer heat, phlegm and cough. According to Compendium of Materia Medica, it is used for insomnia due to deficiency of the spleen and stomach.

[0023] Poria cocos (Schw.) Wolf is the dried sclerotium of Poria cocos (Schw.) Wolf. It is sweet and bland in taste and flat in nature. It is associated with the heart, lung, spleen and kidney meridians. It has the effects of promoting diuresis and removing dampness, tonifying the spleen and soothing the heart. According to Compendium of Materia Medica, it is bland in flavor and permeable, with the nature of ascending, producing body fluid, opening the pores, descending by moistening water, promoting diuresis, and therefore Zhang Jieguguo said that it belongs to yang and floats upward, and Dongyuan said that it is the yin within yang and descends downward.

[0024] Polygonatum odoratum is flat in nature and sweet in taste. It is associated with the lung and stomach meridians. It has the effects of nourishing yin and moistening dryness, and relieving thirst. It is used for yin deficiency of the lung and stomach, dryness and heat cough, dry throat and thirst, and internal heat and polydipsia. According to Treatise on Herbal, it is used for treating cold and heat, internal deficiency, deficiency and excessive heat, headache and restlessness.

[0025] Glycyrrhiza uralensis is flat and sweet in taste. It is associated with the stomach, spleen and lung meridians. It has the effects of clearing heat and resolving toxins, tonifying the spleen and benefiting qi, relieving acute and chronic pain, expelling phlegm and relieving cough, detoxifying and regulating the properties of drugs. It is used for warming the middle and relieving acute and chronic pain, moistening the lungs, detoxifying and regulating the properties of drugs. It is used for spleen and stomach weakness, poor appetite, abdominal pain and loose stools, fatigue and fever, lung deficiency and cough, palpitation and convulsions. It is used for treating sore throat, peptic ulcer, carbuncle and ulcer, and drug and food poisoning. According to Miscellaneous Records, it is used for warming the middle and promoting qi, relieving fullness and shortness of breath, treating stomach and lung cough, relieving thirst, promoting blood and qi, and resolving the toxicity of various drugs.

[0026] The beneficial technical effects of the present application are: The present application provides a traditional Chinese medicine preparation for tonifying qi and blood and improving immunity. The cherokee rose and purple bamboo root are the monarch drugs in the prescription. The cherokee rose has the effects of consolidating essence, shrinking urine, stopping bleeding and relieving diarrhea. The purple bamboo root has the effects of expelling wind and dampness and strengthening bones and muscles. The gold stone dendrobium, fructus terminaliae belericae and angelica are the minister drugs. The gold stone dendrobium benefits the stomach and produces body fluid, the fructus terminaliae belericae promotes blood circulation and unblocks the meridians, and the angelica tonifies blood and moistens the intestines. The prepared rhizome of Chinese wolfberry, elm leaf, poria cocos and polygonatum odoratum are the auxiliary drugs. The prepared rhizome of Chinese wolfberry nourishes yin and benefits qi, the elm leaf clears heat and soothes the nerves, the poria cocos promotes diuresis and removes dampness, tonifies the spleen and soothes the heart, and the polygonatum odoratum nourishes yin and moistens dryness. The glycyrrhiza regulates the properties of various drugs. The compatibility of the drugs in the prescription is reasonable. The traditional Chinese medicine preparation tonifies qi and blood, produces body fluid and nourishes blood, and has the effects of tonifying qi and blood, which enhances the immunity of the body. DETAILED DESCRIPTION

[0027] The following further describes the present application in connection with specific preferred embodiments, which should not be construed as limiting the present application to only these described embodiments. Those skilled in the art with access to the teachings provided herein will recognize additional implementations, modifications, and embodiments, which are also intended to fall within the scope of the present application. Unless otherwise indicated, the specific conditions in the examples were carried out according to conventional conditions or according to the manufacturer's recommendations. The reagents or instruments used were conventional products, which were obtained from commercial sources, unless otherwise indicated.

[0028] Example 1 A Chinese medicinal preparation for tonifying Qi and blood and improving immunity comprises the following ingredients by weight: 15 parts of Chamaerops humilis, 8 parts of Phyllostachys nigra, 10 parts of Dendrobium nobile, 10 parts of Amomum kravanh, 8 parts of Angelica sinensis, 7 parts of Radix Rehmanniae Preparata, 9 parts of Ulmus, 5 parts of Poria cocos, 8 parts of Polygonatum, and 2 parts of Glycyrrhiza uralensis.

[0029] The present embodiment also provides a preparation method of the Chinese medicinal preparation for tonifying Qi and blood and improving immunity, which comprises the following steps: (1) Each raw medicinal material is taken according to the weight, and Chamaerops humilis, Phyllostachys nigra, Dendrobium nobile, Amomum kravanh, Angelica sinensis, Radix Rehmanniae Preparata, Ulmus, Poria cocos, Polygonatum, and Glycyrrhiza uralensis are soaked in water for 25 min and decocted for 4 h according to a solid-liquid ratio of 1 g:10 mL, and then filtered to obtain filtrate A and residue A; (2) The residue A after filtration in step (1) is added with water again for decoction for 2 h according to a solid-liquid ratio of 1 g:8 mL, and then filtered to collect filtrate B and residue B; (3) The filtrate A and the filtrate B are combined, concentrated to a relative density of 1.1 extract, and then 2% starch by mass of the extract is added, and the mixture is formed into tablets according to a conventional method to obtain the product.

[0030] Example 2 A Chinese medicinal preparation for tonifying Qi and blood and improving immunity comprises the following ingredients by weight: 12 parts of Chamaerops humilis, 5 parts of Phyllostachys nigra, 8 parts of Dendrobium nobile, 8 parts of Amomum kravanh, 7 parts of Angelica sinensis, 6 parts of Radix Rehmanniae Preparata, 5 parts of Ulmus, 3 parts of Poria cocos, 5 parts of Polygonatum, and 1 part of Glycyrrhiza uralensis.

[0031] The present embodiment also provides a preparation method of the Chinese medicinal preparation for tonifying Qi and blood and improving immunity, which comprises the following steps: (1) Each raw medicinal material is taken according to the weight, and Chamaerops humilis, Phyllostachys nigra, Dendrobium nobile, Amomum kravanh, Angelica sinensis, Radix Rehmanniae Preparata, Ulmus, Poria cocos, Polygonatum, and Glycyrrhiza uralensis are soaked in water for 20 min and decocted for 3 h according to a solid-liquid ratio of 1 g:8 mL, and then filtered to obtain filtrate A and residue A; (2) The solid-liquid ratio of the filter residue A and water after filtration according to step (1) is 1 g:5 mL. The filter residue A after filtration in step (1) is boiled with water again for 1 h, filtered, and the filtrate B and filter residue B are collected; (3) The filtrate A and the filtrate B are combined, concentrated to a relative density of 1.0 extract, and 2% starch of the mass of the extract is added. The tablet is prepared according to a conventional method to obtain the product.

[0032] Example 3 A traditional Chinese medicine preparation for tonifying qi and blood and improving immunity comprises the following raw materials by weight: 17 parts of Chamaerops humilis, 10 parts of purple bamboo root, 12 parts of Dendrobium nobile, 12 parts of Amomum kravanh, 10 parts of Angelica sinensis, 9 parts of Radix Rehmanniae Preparata, 10 parts of Ulmus, 7 parts of Poria cocos, 10 parts of Polygonatum, and 4 parts of licorice.

[0033] The embodiment also provides a preparation method of the traditional Chinese medicine preparation for tonifying qi and blood and improving immunity, which comprises the following steps: (1) The raw materials are taken according to the weight, and the solid-liquid ratio of the total mass of the raw materials and water is 1 g:12 mL. Chamaerops humilis, purple bamboo root, Dendrobium nobile, Amomum kravanh, Angelica sinensis, Radix Rehmanniae Preparata, Ulmus, Poria cocos, Polygonatum, and licorice are soaked in water for 30 min and boiled for 5 h. Filtration is performed to obtain the filtrate A and the filter residue A. (2) The solid-liquid ratio of the filter residue A after filtration according to step (1) and water is 1 g:10 mL. The filter residue A after filtration in step (1) is boiled with water again for 3 h, filtered, and the filtrate B and filter residue B are collected. (3) The filtrate A and the filtrate B are combined, concentrated to a relative density of 1.2 extract, and 2% starch of the mass of the extract is added. The tablet is prepared according to a conventional method to obtain the product.

[0034] Comparative Example 1 Comparative Example 1 and Example 1 are basically the same, except that the purple bamboo root is replaced by Chamaerops humilis, and the other conditions are the same as those in Example 1.

[0035] Comparative Example 2 Comparative Example 2 and Example 1 are basically the same, except that Amomum kravanh is omitted, and the other conditions are the same as those in Example 1.

[0036] Comparative Example 3 Comparative Example 3 and Example 1 are basically the same, except that Ulmus is omitted, and the other conditions are the same as those in Example 1.

[0037] Test Example In order to verify the curative effect of the traditional Chinese medicine preparations obtained in Example 1 and Comparative Examples 1-3, the following test is performed: 1. Experimental Animals: 240 male SPF-grade Kunming mice, weighing 18-22g, were selected and housed in a clean-grade laboratory at a temperature of approximately 25℃ and a humidity of 50-60%. A 12-hour day-night cycle was implemented, with light from 08:00 to 20:00 and darkness from 20:00 to 08:00. Free access to water and food was provided for 7 days to allow for acclimatization.

[0038] 2. Animal grouping: The Kunming mice were randomly divided into three batches of 80 mice each. The first batch was used for measuring mouse organ indices, the second batch for delayed-type hypersensitivity experiments, and the third batch for ConA-induced lymphocyte transformation experiments. Each batch was further divided into the following groups: Example 1 high-dose group, Example 1 medium-dose group, Example 1 low-dose group, Comparative Example 1 group, Comparative Example 2 group, Comparative Example 3 group, blank control group, and model group.

[0039] 3. Establishment and administration of immunocompromised mouse model: Except for the blank control group, all ICR mice in the group were intraperitoneally injected with cyclophosphamide (CTX) at a dose of 80 mg / kg once a day for 5 consecutive days.

[0040] After 5 consecutive days of administration, the low-dose group of Example 1 was administered the traditional Chinese medicine preparation of Example 1 by gavage at a dose of 5 g / kg (calculated as crude drug); once a day for 30 consecutive days. In Example 1, the dosage group was administered the traditional Chinese medicine preparation of Example 1 by gavage at a dose of 10 g / kg (calculated as crude drug); once a day for 30 consecutive days. Example 1 High-dose group: The traditional Chinese medicine preparation of Example 1 was administered by gavage at a dose of 20 g / kg (calculated as crude drug); once a day for 30 consecutive days; High-dose group of Comparative Example 1: The traditional Chinese medicine preparation of Comparative Example 1 was administered by gavage at a dose of 20 g / kg (calculated as crude drug); once a day for 30 consecutive days; High-dose group of Comparative Example 2: The traditional Chinese medicine preparation of Comparative Example 2 was administered by gavage at a dose of 20 g / kg (calculated as crude drug); once a day for 30 consecutive days; High-dose group of Comparative Example 3: The traditional Chinese medicine preparation of Comparative Example 3 was administered by gavage at a dose of 20 g / kg (calculated as crude drug); once a day for 30 consecutive days; Both the blank control group and the model group were given the same amount of purified water, once a day for 30 consecutive days. 4. Indicator Testing 4.1 Mouse organ index detection: 12 hours after the last administration, mice were fasted for 12 hours, and their body weight was measured the following day. The mice were then sacrificed, and their spleen and thymus were harvested, weighed, and recorded. The spleen and thymus indices were calculated. The formulas are as follows: Mouse spleen index = spleen mass (mg) / mouse body weight (g); Mouse thymus index = thymus mass (mg) / mouse body weight (g). The experimental results are shown in Table 1.

[0041] Table 1 The spleen and thymus are important immune organs, and changes in their quality can reflect the effects of drugs on the immune system. Table 1 shows that, compared to the blank control group, the spleen and thymus indices of the mice in the model group were significantly lower, indicating successful modeling. Compared to the model group, the spleen and thymus indices of the traditional Chinese medicine preparations in the low, medium, and high dose groups of Example 1 of this invention were all increased. Compared to the high dose group of Example 1, Comparative Example 1 replaced the root of *Phyllostachys nigra* with *Rosa laevigata*, Comparative Example 2 omitted *Tetrapanax papyriferus* fruit, and Comparative Example 3 omitted *Elm tung oil* leaf. Compared to the high dose group of Example 1, the spleen and thymus indices of the high dose groups in Comparative Examples 1-3 were all lower, indicating poor efficacy.

[0042] 4.2 Lymphocyte proliferation assay: After the last administration, mouse spleens were aseptically removed and placed in Hank's solution, then ground to prepare a single-cell suspension. The cell concentration was adjusted to 3 × 10⁻⁶ cells using RPMI 1640 cell culture medium. 6 Cells / mL. Each group of cell suspensions was divided into two wells and added to 24-well plates. One well contained 75 µL of 100 µg / mL ConA solution, and the other well contained an equal volume of FBS-free RPMI 1640 cell culture medium as a control. Each group had three replicates. The plates were incubated at 37°C with 5% CO2 for 72 h. Four hours before the end of incubation, 0.7 mL of supernatant was aspirated from each well, and 0.7 mL of FBS-free RPMI 1640 cell culture medium was added. Simultaneously, 50 μL of MTT (5 mg / mL) was added to each well, and the plates were incubated for another 4 h. After incubation, 1 mL of acidic isopropanol was added to each well, and the mixture was pipetted to dissolve the purple crystals. The optical density (OD) value of each well was measured at 570 nm. Lymphocyte proliferation capacity = ConA well OD - Control well OD. Results are shown in Table 2.

[0043] Table 2 The stronger the lymphocyte proliferation capacity, the higher the immunity. Table 2 shows that, compared with the model group, the low, medium, and high dose groups of the traditional Chinese medicine preparation in Example 1 of this invention can enhance the lymphocyte proliferation capacity, and the immunity of mice is significantly improved. Compared with the high dose group of Example 1, the lymphocyte proliferation capacity of the high dose groups in Comparative Examples 1-3 decreased to varying degrees, and the effect was not as good as that of the high dose group in Example 1.

[0044] 4.3 Mouse Carbon Clearance Experiment: After the last administration, the Indian ink stock solution was diluted 3 times with physiological saline to obtain the Indian ink working solution. Mice were injected with the Indian ink working solution via tail vein at a dose of 10 mL / kg. 2 min (t1) and 10 min (t2) after injection, 20 μL of blood was collected from the internal canthal venous plexus and immediately added to 2 mL of 0.1% Na2CO3 solution. Using the Na2CO3 solution as a blank control, the optical density values ​​at 600 nm wavelength were measured at 2 min and 10 min after injection and recorded as OD1 and OD2, respectively. Mice were sacrificed, and their livers and spleens were harvested and weighed. The phagocytic index was calculated, representing the mouse's carbon clearance capacity. The clearance index K = (lgOD1 - lgOD2) / (t2 - t1), and the phagocytic index = body weight ÷ (liver weight + spleen weight) × .

[0045] Table 3 As shown in Table 3, the phagocytic index of the model group mice was decreased compared with the blank control group. Compared with the model group, the phagocytic index of the mice in the low, medium, and high dose groups of Example 1 was increased. This indicates that the traditional Chinese medicine preparation obtained in this invention can improve the phagocytic function of mononuclear-macrophages in mice and enhance the immunity of immunocompromised mice. Although the phagocytic index of the high dose groups in Comparative Examples 1-3 was increased compared with the model group, the effect was not as good as that of the high dose group in Example 1.

[0046] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them. The basic principles and main features of the present invention have been described above with specific implementation schemes. Based on the present invention, some modifications or substitutions can be made, but these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of protection claimed by the present invention.

Claims

1. A traditional Chinese medicine preparation for replenishing qi and blood and enhancing immunity, characterized in that, By weight, it includes the following ingredients: 12-17 parts of Rosa laevigata, 5-10 parts of Rhizoma Phyllostachys nigra, 8-12 parts of Dendrobium nobile, 8-12 parts of Tinospora sinensis fruit, 7-10 parts of Angelica sinensis, 6-9 parts of Rehmanniae Radix Rehmanniae, 5-10 parts of Folium Ulmi, 3-7 parts of Poria cocos, 5-10 parts of Radix Polygonati Rhizoma, and 1-4 parts of Radix Glycyrrhizae.

2. The traditional Chinese medicine preparation for replenishing qi and blood and enhancing immunity according to claim 1, characterized in that, By weight, it includes the following ingredients: 15 parts of Rosa laevigata, 8 parts of Rhizoma Phyllostachys nigra, 10 parts of Dendrobium nobile, 10 parts of Tinospora sinensis fruit, 8 parts of Angelica sinensis, 7 parts of Rehmanniae Radix Rehmanniae, 9 parts of Folium Ulmi, 5 parts of Poria cocos, 8 parts of Radix Polygonati Rhizoma, and 2 parts of Radix Glycyrrhizae.

3. The method for preparing the traditional Chinese medicine preparation for replenishing qi and blood and enhancing immunity according to claim 1 or 2, characterized in that, Includes the following steps: (1) Take each raw material according to the weight parts, soak the following ingredients in water and decoct them: Rosa laevigata, Rhizoma Zingiberis indicum, Dendrobium nobile, Tetrapanax papyriferus, Angelica sinensis, Rehmanniae Radix Praeparata, Ulmus pumila, Poria cocos, Polygonatum odoratum, and Glycyrrhiza uralensis. Filter to obtain filtrate A and residue A. (2) Add water to the filter residue A after filtering in step (1) again, boil it, filter it, and collect the filtrate B and the filter residue B; (3) Combine filtrate A and filtrate B, concentrate them into an extract, and prepare the extract into a corresponding dosage form with pharmaceutically acceptable excipients to obtain the product.

4. The method for preparing the traditional Chinese medicine preparation for replenishing qi and blood and enhancing immunity according to claim 3, characterized in that, In step (1), the total mass of the raw material and the solid-liquid ratio of water are 1g:(8-12)mL.

5. The method for preparing the traditional Chinese medicine preparation for replenishing qi and blood and enhancing immunity according to claim 3, characterized in that, In step (1), the soaking time is 20-30 minutes.

6. The method for preparing the traditional Chinese medicine preparation for replenishing qi and blood and enhancing immunity according to claim 3, characterized in that, In step (2), the solid-liquid ratio of the filter residue A and water is 1g:(5-10)mL.

7. The method for preparing the traditional Chinese medicine preparation for replenishing qi and blood and enhancing immunity according to claim 3, characterized in that, The decocting time in step (1) is 3-5 hours, and the decocting time in step (2) is 1-3 hours.

8. The method for preparing the traditional Chinese medicine preparation for replenishing qi and blood and enhancing immunity according to claim 3, characterized in that, The relative density of the extract in step (3) is 1.0-1.

2.

9. The method for preparing the traditional Chinese medicine preparation for replenishing qi and blood and enhancing immunity according to claim 3, characterized in that, The dosage form is a tablet.

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