Dietary therapy composition for promoting repair of cranial nerve damaged cells and preparation method thereof

Abstract

The invention relates to the technical field of functional foods, and particularly discloses a dietary therapy composition for promoting repair of cranial nerve damaged cells and a preparation method. The composition is prepared from the following raw materials in parts by weight: artichoke peptide, taurine, phosphatidylserine, natural nicotinic acid, guarana extract, collagen peptide, acerola cherry fruit powder, inulin, yak bone marrow powder, vitamin B family, sialic acid and sucralose. The preparation method comprises the following steps: S1, pretreating the raw materials; s2, performing nano crushing; s3, low-temperature drying; s4, embedding the inulin; and S5, mixing and forming. The artichoke peptide and the phosphatidylserine are synergistically matched, and a compound component with liver and brain axis functions is taken into consideration, so that a multi-target synergistic effect is achieved, the requirements of cell repair and neural development are synchronously met, and compared with single-component supplement or neglect of the influence of liver detoxification on brain repair in the prior art, the brain repair effect is greatly improved. The problems of low repairing efficiency and insufficient component utilization rate are solved.

Description

Technical Field

[0001] This invention relates to the field of functional food technology, specifically to a dietary composition and preparation method for promoting the repair of damaged brain nerve cells. Background Technology

[0002] In the functional food sector, products targeting delayed brain development or cell damage have gradually become a research hotspot. Currently, the mainstream intervention methods mainly include three categories: drug therapy, ordinary nutritional supplements, and traditional dietary therapy. Drug therapy often uses ingredients such as nerve growth factor and brain protein hydrolysates; ordinary nutritional supplements mainly consist of single ingredients such as B vitamins and DHA; and traditional dietary therapy commonly uses ingredients such as walnuts and sesame seeds. However, these products all have significant limitations: drug therapy has significant side effects and is costly; ordinary nutritional supplements have limited ingredients and poor synergistic effects; and traditional dietary therapy has low levels of active ingredients and poor bioavailability. Furthermore, existing products generally do not focus on the bile secretion-promoting effect of artichoke peptides, failing to leverage this characteristic to improve the absorption efficiency of fat-soluble components such as phosphatidylserine, and neglecting the important role of liver detoxification in clearing neurotoxins from the brain. This results in core components being easily metabolized by the liver through first-pass metabolism, further reducing the actual efficacy of the products.

[0003] Existing technologies mostly employ single-component supplementation solutions, which cannot achieve synergistic effects on multiple targets. Furthermore, by neglecting the supporting role of liver detoxification in brain repair, the utilization rate of core active ingredients is greatly reduced, ultimately resulting in low product repair efficiency and failing to meet users' actual needs for brain nerve repair. Summary of the Invention

[0004] To address the shortcomings of existing technologies, this invention provides a dietary therapy composition and preparation method for promoting the repair of damaged brain nerve cells. This solves the problems of low repair efficiency and insufficient component utilization caused by existing technologies that rely on supplementing with a single component or neglecting the impact of liver detoxification on brain repair.

[0005] To achieve the above objectives, the present invention is implemented through the following technical solution: a dietary therapy composition for promoting the repair of damaged brain nerve cells, comprising the following raw materials in parts by weight: 3-10 parts of artichoke peptide, 10-30 parts of taurine, 2-4 parts of phosphatidylserine, 0.01-0.03 parts of natural niacin, 0.5-1 parts of guarana extract, 30-42 parts of collagen peptide, 3-5 parts of acerola cherry fruit powder, 5-15 parts of inulin, 3-5 parts of yak bone marrow powder, 0.01-0.3 parts of vitamin B complex, 1-3 parts of sialic acid, and 0.1-2 parts of sucralose.

[0006] Preferably, the collagen peptide is an animal bone-derived active peptide with a molecular weight of 500-3000 Da, obtained by enzymatic hydrolysis and ultrafiltration purification.

[0007] Preferably, the artichoke peptide is an extract of artichoke receptacle, the purity of the artichoke peptide is ≥90%, and it is obtained by water extraction and compound enzymatic hydrolysis purification. The natural nicotinic acid is derived from quinoline acid in acerola cherry fruit powder.

[0008] Preferably, the acerola cherry fruit powder is made from fresh acerola cherry fruit, which is washed, pitted, freeze-dried at -40 to -30°C, and pulverized, and contains natural quinolinic acid.

[0009] A dietary therapy composition for promoting the repair of damaged brain nerve cells and its preparation method, comprising the following steps: S1. Raw material pretreatment: Each raw material in the composition except for inulin is subjected to impurity removal treatment and then dried to obtain pretreated raw materials; S2. Nanoparticle pulverization: After the pretreated raw materials are mixed evenly, they are put into a nano airflow pulverizer for pulverization to obtain nano-scale mixed powder. S3. Low-temperature drying: The nano-grade mixed powder is dried at low temperature using a gradient heating method to remove residual moisture from the raw materials. Inert gas is used for protection during the drying process. S4. Inulin encapsulation: The dried mixed powder is mixed with inulin and encapsulated using a spray drying process to form encapsulated particles; S5. Mixing and Molding: After mixing the embedded particles, they are processed into finished products using molding equipment.

[0010] Preferably, in step S1, the impurity removal process employs a combination of screening and magnetic separation to remove mechanical and magnetic impurities from the raw materials. The drying process is carried out at a temperature of 40-45℃ for 1-2 hours to ensure that the moisture content of the raw materials is ≤5%.

[0011] Preferably, in S2, the nano airflow pulverizer is of type KQW, the working pressure of the equipment during the pulverization process is 0.7-0.85MPa, the working power is 1600kW, the particle size of the nano-scale mixed powder after pulverization is 100-300nm, and the particle size distribution uniformity deviation is ≤15%.

[0012] Preferably, in step S3, the low-temperature drying process adopts a gradient heating method, with an initial temperature of 48-52℃, gradually increasing to 53-57℃, and a total drying time of 2-4 hours; In step S3, the initial temperature of the gradient heating is 48-52℃, which is gradually increased to 53-57℃, and the total drying time is 2-4 hours.

[0013] Preferably, in step S4, the inlet temperature of the spray drying process is 120-140℃, the outlet temperature is 60-70℃, the atomization pressure is 0.3-0.5MPa, and the particle size of the encapsulated particles is 80-120nm.

[0014] Preferably, in step S5, the mixing process uses a horizontal mixer with a mixing speed of 300-500 r / min, a mixing time of 15-30 minutes, and a mixing uniformity ≥95%. The forming equipment is a tablet press or a granulator, which processes the product into tablets or granules. The disintegration time of the finished product is 15-30 minutes.

[0015] This invention provides a dietary composition and preparation method for promoting the repair of damaged brain nerve cells. It has the following beneficial effects: 1. This invention, through the synergistic combination of artichoke peptide and phosphatidylserine, and the design of a composite component that takes into account the function of the liver-brain axis, achieves the technical effect of multi-target synergistic action and simultaneously meets the needs of cell repair and neural development. Compared with the existing technical solutions that supplement with a single component or ignore the impact of liver detoxification on brain repair, this invention solves the problems of low repair efficiency and insufficient component utilization.

[0016] 2. This invention employs a combination of nano-airflow pulverization and inulin encapsulation technology, which achieves the technical effect of optimizing component particle size and improving the bioavailability of lipid-soluble components. Compared with the existing technology of ordinary pulverization or non-targeted encapsulation, it solves the problem that the active ingredients are difficult to penetrate the blood-brain barrier and have poor bioavailability due to their large molecular weight.

[0017] 3. This invention uses acerola cherry fruit powder to provide a natural niacin precursor, combined with gradient heating and inert gas protection for low-temperature drying stability technology, which achieves the technical effect of reducing food side effects and protecting heat-sensitive active ingredients. Compared with the existing technology that uses chemically synthesized niacin or lacks effective ingredient protection, it solves the problems of easy adverse reactions and easy oxidation and degradation of active ingredients. Attached Figure Description

[0018] Figure 1 This is a schematic diagram of the steps involved in the preparation of a dietary therapy composition for promoting the repair of damaged brain nerve cells according to the present invention. Detailed Implementation

[0019] The technical solution of the present invention will now be clearly and completely described with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0020] This invention provides a dietary therapy composition for promoting the repair of damaged brain nerve cells, comprising the following ingredients in parts by weight: 3-10 parts artichoke peptide, 10-30 parts taurine, 2-4 parts phosphatidylserine, 0.01-0.03 parts natural niacin, 0.5-1 parts guarana extract, 30-42 parts collagen peptide, 3-5 parts acerola cherry fruit powder, 5-15 parts inulin, 3-5 parts yak bone marrow powder, 0.01-0.3 parts vitamin B complex, 1-3 parts sialic acid, and 0.1-2 parts sucralose.

[0021] Collagen peptides are animal bone-derived active peptides with a molecular weight of 500-3000 Da, obtained through enzymatic hydrolysis and ultrafiltration purification.

[0022] Artichoke peptide is an extract of artichoke receptacle. The purity of artichoke peptide is ≥90%. It is obtained by water extraction and compound enzymatic hydrolysis purification. Natural nicotinic acid comes from quinoline acid in acerola cherry fruit powder.

[0023] Acerola cherry fruit powder is made from fresh acerola cherry fruit, which is washed, pitted, freeze-dried at -40 to -30℃, and then pulverized. It contains natural quinolinic acid.

[0024] Please see the appendix Figure 1 A dietary therapy composition for promoting the repair of damaged brain nerve cells and its preparation method, comprising the following steps: S1. Raw material pretreatment: Each raw material in the composition except for inulin is subjected to impurity removal treatment and then dried to obtain pretreated raw materials; Among them, the impurity removal process adopts a combination of screening and magnetic separation to remove mechanical and magnetic impurities from the raw materials; The drying temperature is controlled at 40-45℃, and the drying time is 1-2 hours to ensure that the moisture content of the raw material is ≤5%.

[0025] S2. Nanoparticle pulverization: After the pretreated raw materials are mixed evenly, they are put into a nano airflow pulverizer for pulverization to obtain nano-scale mixed powder. Among them, the nano airflow pulverizer is of type KQW. During the pulverization process, the working pressure of the equipment is 0.7-0.85MPa and the working power is 1600kW. The particle size of the nano-grade mixed powder after pulverization is 100-300nm, and the particle size distribution uniformity deviation is ≤15%.

[0026] S3. Low-temperature drying: The nano-grade mixed powder is dried at low temperature using a gradient heating method to remove residual moisture from the raw materials. Inert gas is used for protection during the drying process. The low-temperature drying process uses a gradient heating method, with an initial temperature of 48-52℃, gradually increasing to 53-57℃, and a total drying time of 2-4 hours. The initial temperature for gradient heating is 48-52℃, gradually increasing to 53-57℃, with a total drying time of 2-4 hours.

[0027] S4. Inulin encapsulation: The dried mixed powder is mixed with inulin and encapsulated using a spray drying process to form encapsulated particles; The spray drying process has an inlet temperature of 120-140℃, an outlet temperature of 60-70℃, an atomization pressure of 0.3-0.5MPa, and an encapsulated particle size of 80-120nm.

[0028] S5. Mixing and molding: After mixing the embedded particles, they are processed into finished products using molding equipment. The mixing process employs a horizontal mixer with a mixing speed of 300-500 r / min and a mixing time of 15-30 minutes, achieving a mixing uniformity of ≥95%. The forming equipment is a tablet press or a granulator, which processes the product into tablets or granules. The disintegration time of the finished product is 15-30 minutes.

[0029] The following is a description with reference to specific embodiments: Example 1: A dietary composition for promoting the repair of damaged brain nerve cells, comprising the following ingredients in parts by weight: Artichoke peptide 5 parts, taurine 10 parts, phosphatidylserine 2 parts, natural niacin 0.01 parts, guarana extract 0.5 parts, collagen peptide 30 parts, acerola cherry fruit powder 3 parts, inulin 5 parts, yak bone marrow powder 3 parts, vitamin B complex 0.01 parts, sialic acid 1 part, sucralose 0.1 parts; Collagen peptides: Animal bone-derived active peptides with a molecular weight of 500 Da, obtained through enzymatic hydrolysis and ultrafiltration purification; Artichoke peptide: Artichoke receptacle extract, 90% purity, obtained by water extraction and compound enzymatic hydrolysis purification; Natural niacin: derived from quinolinic acid in acerola cherry fruit powder, obtained by washing, pitting, freeze-drying at -40℃, and pulverizing; Guarana extract: prepared from fresh guarana fruit through washing, drying, pulverizing, ethanol extraction, concentration and drying; Sucralose: Food grade, purity conforms to GB25531-2010 standard.

[0030] A dietary therapy composition for promoting the repair of damaged brain nerve cells and its preparation method, comprising the following steps: S1. Raw material pretreatment: Except for inulin, each raw material is removed by a combination of screening and magnetic separation to remove mechanical and magnetic impurities; then dried at 40℃ for 1 hour to obtain pretreated raw materials with a moisture content controlled within 5%. S2. Nanoparticle pulverization: After the pretreated raw materials are mixed evenly, they are fed into a KQW type nano airflow pulverizer and pulverized under working pressure of 0.7MPa and working power of 1600kW to obtain nano-sized mixed powder with a particle size of 100nm and a particle size distribution uniformity deviation of 15%. S3. Low-temperature drying: The nano-sized mixed powder is dried at a low temperature using a gradient heating method. The initial temperature is 48℃, and the temperature is gradually increased to 53℃. The total drying time is 2 hours. Inert gas protection is used during the drying process. S4. Inulin encapsulation: The dried mixed powder is mixed with inulin according to the formula ratio, distilled water is added and stirred to dissolve, and the powder is encapsulated using a spray drying process with an inlet temperature of 120℃, an outlet temperature of 60℃, and an atomization pressure of 0.3MPa to form encapsulated particles with a particle size of 80nm. S5. Mixing and molding: The encapsulated particles are put into a horizontal mixer and mixed at 300r / min for 15 minutes, with a mixing uniformity of 95%; then processed into tablets by a tablet press, with a disintegration time of 15 minutes.

[0031] Example 2: A dietary composition for promoting the repair of damaged brain nerve cells, comprising the following ingredients in parts by weight: Artichoke peptide 8 parts, taurine 20 parts, phosphatidylserine 3 parts, natural niacin 0.02 parts, guarana extract 0.8 parts, collagen peptide 38 parts, acerola cherry fruit powder 4 parts, inulin 10 parts, yak bone marrow powder 4 parts, vitamin B complex 0.02 parts, sialic acid 2 parts, sucralose 1.1 parts; Collagen peptides: Animal bone-derived active peptides with a molecular weight of 2000 Da, obtained through enzymatic hydrolysis and ultrafiltration purification; Artichoke peptide: Artichoke receptacle extract, 95% purity, obtained by water extraction and compound enzymatic hydrolysis purification; Natural niacin: derived from quinolinic acid in acerola cherry fruit powder, obtained by washing, pitting, freeze-drying at -35℃, and pulverizing; Guarana extract: prepared from fresh guarana fruit through washing, drying, pulverizing, ethanol extraction, concentration and drying; Sucralose: Food grade, purity conforms to GB25531-2010 standard.

[0032] A dietary composition containing artichoke peptides to promote brain nerve repair, comprising the following steps: S1. Raw material pretreatment: Except for inulin, each raw material is removed by a combination of screening and magnetic separation to remove mechanical and magnetic impurities; then dried at 42℃ for 1.5 hours to obtain pretreated raw materials with a moisture content controlled within 5%; S2. Nanoparticle pulverization: After the pretreated raw materials are mixed evenly, they are fed into a KQW type nano airflow pulverizer and pulverized under working pressure of 0.8MPa and working power of 1600kW to obtain nano-sized mixed powder with a particle size of 200nm and a particle size distribution uniformity deviation of 12%. S3. Low-temperature drying: The nano-sized mixed powder is dried at a low temperature using a gradient heating method. The initial temperature is 50℃, and the temperature is gradually increased to 55℃. The total drying time is 3 hours. Inert gas protection is used during the drying process. S4. Inulin encapsulation: The dried mixed powder is mixed with inulin according to the formula ratio, distilled water is added and stirred to dissolve, and the powder is encapsulated using a spray drying process with an inlet temperature of 130℃, an outlet temperature of 65℃, and an atomization pressure of 0.4MPa to form encapsulated particles with a particle size of 100nm. S5. Mixing and molding: The encapsulated particles are put into a horizontal mixer and mixed at 400 r / min for 20 minutes, with a mixing uniformity of 97%; then processed into granular finished products by a granulator, with a disintegration time limit of 20 minutes.

[0033] Example 3: A dietary composition for promoting the repair of damaged brain nerve cells, comprising the following ingredients in parts by weight: Artichoke peptide 10 parts, taurine 30 parts, phosphatidylserine 4 parts, natural niacin 0.03 parts, guarana extract 0.1 parts, collagen peptide 42 parts, acerola cherry fruit powder 5 parts, inulin 15 parts, yak bone marrow powder 5 parts, vitamin B complex 0.03 parts, sialic acid 3 parts, sucralose 2 parts. Collagen peptides: Animal bone-derived active peptides with a molecular weight of 3000 Da, obtained through enzymatic hydrolysis and ultrafiltration purification; Artichoke peptide: Artichoke receptacle extract, 98% purity, obtained by water extraction and compound enzymatic hydrolysis purification; Natural niacin: derived from quinolinic acid in acerola cherry fruit powder, obtained by washing, pitting, freeze-drying at -30℃, and pulverizing; Guarana extract: prepared from fresh guarana fruit through washing, drying, pulverizing, ethanol extraction, concentration and drying; Sucralose: Food grade, purity conforms to GB25531-2010 standard.

[0034] A dietary therapy composition for promoting the repair of damaged brain nerve cells and its preparation method, comprising the following steps: S1. Raw material pretreatment: Except for inulin, each raw material is removed by a combination of screening and magnetic separation to remove mechanical and magnetic impurities; then dried at 45℃ for 2 hours to obtain pretreated raw materials with a moisture content controlled within 5%. S2. Nanoparticle pulverization: After the pretreated raw materials are mixed evenly, they are fed into a KQW type nano airflow pulverizer and pulverized under working pressure of 0.85MPa and working power of 1600kW to obtain nano-sized mixed powder with a particle size of 300nm and a particle size distribution uniformity deviation of 10%. S3. Low-temperature drying: The nano-sized mixed powder is dried at a low temperature using a gradient heating method. The initial temperature is 52℃, and the temperature is gradually increased to 57℃. The total drying time is 4 hours. Inert gas protection is used during the drying process. S4. Inulin encapsulation: The dried mixed powder is mixed with inulin according to the formula ratio, distilled water is added and stirred to dissolve, and the powder is encapsulated using a spray drying process with an inlet temperature of 140℃, an outlet temperature of 70℃, and an atomization pressure of 0.5MPa to form encapsulated particles with a particle size of 120nm. S5. Mixing and molding: The encapsulated particles are put into a horizontal mixer and mixed at 500 r / min for 30 minutes, with a mixing uniformity of 99%; then processed into tablets by a tablet press, with a disintegration time of 30 minutes.

[0035] Comparative Example 1: Unlike Example 2, the impurity removal process of the raw material pretreatment only adopted screening and did not perform magnetic separation. The remaining process parameters were the same as those in Example 2.

[0036] Comparative Example 2: Unlike Example 2, the nano-pulverization did not use the KQW type nano-airflow pulverizer, but instead used a regular high-speed pulverizer. All other process parameters were the same as in Example 2.

[0037] Comparative Example 3: Unlike Example 2, the low-temperature drying did not use a gradient heating method, but was directly dried at a constant temperature of 55°C, and no inert gas protection was used during the drying process. All other process parameters were the same as in Example 2.

[0038] Comparative Example 4: Unlike Example 2, the inulin encapsulation process was not carried out using spray drying, but instead used freeze drying. All other process parameters were the same as in Example 2.

[0039] Comparative Example 5: Unlike Example 2, the mixing process for the mixed molding was not carried out using a horizontal mixer, but a vertical mixer was used instead. All other process parameters were the same as in Example 2.

[0040] Comparative Example 6: Unlike Example 2, the inulin encapsulation step was omitted in the preparation process. The nano-sized mixed powder dried at low temperature was mixed with distilled water and then directly mixed and shaped. All other process parameters were the same as in Example 2.

[0041] Table 1, Performance Test Data Table

[0042] Based on the differences in Examples 1-3, Comparative Examples 1-6, and the performance test data table, it can be seen that the core advantage of this invention stems from the synergistic effect of six key processes: raw material pretreatment screening and magnetic separation, KQW-type nano-airflow pulverization, gradient heating, low-temperature drying under inert gas protection, spray drying inulin encapsulation, precise mixing with a horizontal mixer, and full-process parameter adaptation. The precise matching of parameters in each step has a significant positive impact on the retention rate of active ingredients, particle size distribution uniformity, mixing uniformity, disintegration time, and impurity residue control of the composition. Furthermore, it achieves a triple synergistic optimization of raw material purity assurance, active ingredient protection, and in vivo absorption efficiency.

[0043] Comparative Examples 1-6 all experienced a targeted decline in performance due to the absence or replacement of a single core process: Comparative Example 1 only used screening for raw material pretreatment and impurity removal, without magnetic separation, resulting in increased residual mechanical and magnetic impurities, posing a safety hazard for long-term consumption; Comparative Example 2 did not use the KQW-type nano-airflow pulverizer, but instead used an ordinary high-speed pulverizer, which increased the deviation in the uniformity of the nano-sized mixed powder particle size distribution, reduced the retention rate of active ingredients, and made it difficult for core components to efficiently penetrate the blood-brain barrier; Comparative Example 3's low-temperature drying did not use a gradient heating method and lacked inert gas protection, resulting in severe oxidation and degradation of heat-sensitive active ingredients, thus reducing their activity. The significantly reduced component retention rate weakens the core efficacy of the product. In Comparative Example 4, the inulin encapsulation process was changed to freeze-drying instead of spray drying, resulting in poor particle formation, decreased retention of active ingredients, and prolonged disintegration time, affecting the efficiency of in vivo dissolution and absorption. In Comparative Example 5, the mixing and molding process was changed to vertical mixer instead of horizontal mixer, resulting in reduced mixing uniformity and uneven distribution of core components in the finished product, making it difficult to guarantee the stability of therapeutic effects. In Comparative Example 6, the inulin encapsulation step was omitted, resulting in a lack of protective barrier for active ingredients, a significant decrease in retention rate, and a prolonged disintegration time, fully demonstrating the indispensability of each process step to product performance.

[0044] Examples 1-3 all exhibit excellent performance, fully meeting the core quality requirements of functional foods. Among them, Example 2 achieves the optimal balance of performance, achieving the best fit between component protection, absorption efficiency, and quality stability; Example 1 maintains process stability with minimal parameters, adapting to the needs of small-scale preparation and cost control; Example 3 meets the requirements of large-scale production with maximum parameters, balancing production capacity and product consistency. Together, they verify the rationality and application flexibility of the parameter range of the present invention.

[0045] The adaptability of parameters at each step is crucial for performance assurance: S1, a combination of screening and magnetic separation for impurity removal, a drying temperature of 40-45℃, and a drying time of 1-2 hours precisely control the moisture content and residual impurities of the raw materials, laying a foundation for pure raw materials in subsequent processes; the S2KQW nano-airflow pulverizer, with its 0.7-0.85MPa working pressure and 1600kW working power, adapts to the raw material pulverization requirements, ensuring that the particle size is reduced to 100-300nm, guaranteeing blood-brain barrier permeability; S3, a gradient heating method from 48-52℃ to 53-57℃, and a drying time of 2-4 hours... Drying time and inert gas protection are adapted to the characteristics of heat-sensitive components, avoiding oxidative degradation; the S4 spray drying process with an inlet temperature of 120-140℃, an outlet temperature of 60-70℃, and an atomization pressure of 0.3-0.5MPa ensures inulin encapsulation effect and improves component stability; the S5 mixing speed of 300-500r / min, mixing time of 15-30 minutes, and tableting or granulation methods are adapted to the mixing and finished product usage requirements of encapsulated particles, ensuring uniformity and disintegration efficiency; precise control of raw material ratios throughout the entire process ensures the foundation for the synergistic effect of various active ingredients.

[0046] Example 2 achieves the optimal balance between component protection, absorption efficiency and quality stability through precise adaptation of parameters throughout the entire process; the process synergy system of Examples 1-3 not only avoids performance loss or cost waste caused by parameter boundaries, but also covers the needs of multiple scenarios such as laboratory research and development, small and medium-sized preparation and large-scale mass production.

[0047] In summary, this invention effectively addresses the pain points of traditional brain nerve repair dietary products, such as low retention rate of active ingredients, poor bioavailability, risk of impurity residue, and unstable efficacy. It significantly improves the overall efficacy and safety of the products. Example 2 has the best cost-effectiveness and potential for large-scale production and can be directly applied to the mass production of functional foods. The parameter flexibility of Examples 1-3 can be adapted to various scenarios such as laboratory research and development, special health needs, and small-scale customization. It provides a standardized and replicable technical solution for the research and development and industrial production of safe and efficient brain nerve repair functional foods, and at the same time provides a new path for raw material synergy and process optimization in the field of functional foods.

[0048] The retention rate of active ingredients for artichoke peptides, N-acetylneuraminic acid, and B vitamins was determined according to GB5009.84-2016 "National Food Safety Standard - Determination of B Vitamins in Food" and GB31616-2014 "National Food Safety Standard - Amino Acid Supplements", and the artichoke peptide detection procedure was adapted with reference to GB / T22492-2008 "Soybean Peptide Powder". For the detection, sample pretreatment was performed first. 0.5g of the finished product was added to 50mL of ultrapure water and extracted by ultrasonication for 30 minutes. Then, it was centrifuged at 10000r / min for 15 minutes. The supernatant was filtered through a 0.22μm filter membrane and detected by high performance liquid chromatography (HPLC) with a C18 column of 4.6mm×250mm and 5μm. The mobile phase for the detection of artichoke peptide was methanol-water = 40:60, the flow rate was 1.0mL / min, and the detection wavelength was 280nm. The mobile phase for the detection of N-acetylneuraminic acid was acetonitrile-water = 20:80, the flow rate was 0.8mL / min, and the detection wavelength was 205nm. Vitamin B complex was determined according to the chromatographic conditions specified in GB5009.84-2016. After recording the peak area of ​​each component, the results were calculated according to the formula: active ingredient retention rate = (measured total content / theoretical total content) × 100%.

[0049] The particle size distribution uniformity deviation was tested according to GB / T31740.2-2015 "Determination of Particle Size Distribution of Nanopowders in Nanotechnology - Part 2: Dynamic Light Scattering Method"; During sample preparation, 0.1 g of nano-sized mixed powder was added to 20 mL of deionized water and ultrasonically dispersed for 10 minutes to form a uniform suspension. The dynamic light scattering particle size analyzer was used to continuously test the particle size three times at a temperature of 25℃ and a scattering angle of 90°, with each test lasting 60 seconds. The final result was calculated as particle size distribution uniformity deviation = (standard deviation / average particle size) × 100%.

[0050] The mixing uniformity test was conducted according to Appendix D of GB16740-2014 "National Food Safety Standard for Health Foods": determination of raw material mixing uniformity. During sampling, 10 sampling points were randomly selected from the finished product. 1g of sample was taken from each sampling point and numbered. The samples from each sampling point were pretreated according to the above-mentioned method for detecting the retention rate of active ingredients, with a focus on determining the content of the core ingredient, artichoke peptide. The mixing uniformity was calculated as (maximum content of each sampling point - minimum content of each sampling point) / average content × 100%, and the result was expressed as a percentage.

[0051] The disintegration time limit test was conducted according to Appendix E of GB16740-2014 "National Food Safety Standard for Health Foods" for the determination of disintegration time limit of tablets and granules. Using a disintegrator, a 500mL beaker, and a thermometer, add 500mL of deionized water at 37±1℃ to the beaker. Adjust the lowering height of the disintegrator basket so that the bottom of the basket is 25mm from the bottom of the beaker. Take 6 tablets or 6 portions of granules and place them into the 6 glass tubes of the disintegrator basket. Start the disintegrator and record the time when all samples completely disintegrate without any hard core. This time is the disintegration time limit. For granules, the criterion is that all of them pass through the No. 2 sieve.

[0052] The residual amount of mechanical and magnetic impurities was tested according to GB2762-2022 "National Food Safety Standard - Limits of Contaminants in Food" and GB / T5009.3-2016 "National Food Safety Standard - Determination of Moisture in Food". For mechanical impurity detection, take 100g of sample, sieve it through an 80-mesh standard sieve, collect the residue on the sieve, rinse it with deionized water, dry it at 105℃ to constant weight, and weigh it. For magnetic impurity detection, take 50g of sample, spread it flat on white paper, slowly move a strong magnet on the sample surface, collect the adsorbed magnetic impurities, rinse it with deionized water, dry it to constant weight, and weigh it. The residual amount of impurities is calculated as (mass of mechanical impurities + mass of magnetic impurities) / total sample mass × 10^6, and the result is expressed in mg / kg.

[0053] Table 2, Comparison Table of Example 2 and Existing Methods

[0054] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A dietary therapy composition for promoting the repair of damaged brain nerve cells and its preparation method, characterized in that, The ingredients include the following parts by weight: 3-10 parts artichoke peptide, 10-30 parts taurine, 2-4 parts phosphatidylserine, 0.01-0.03 parts natural niacin, 0.5-1 part guarana extract, 30-42 parts collagen peptide, 3-5 parts acerola cherry fruit powder, 5-15 parts inulin, 3-5 parts yak bone marrow powder, 0.01-0.3 parts vitamin B complex, 1-3 parts sialic acid, and 0.1-2 parts sucralose.

2. The dietary therapy composition and preparation method for promoting the repair of damaged brain nerve cells according to claim 1, characterized in that: The collagen peptides are animal bone-derived active peptides with a molecular weight of 500-3000 Da, obtained through enzymatic hydrolysis and ultrafiltration purification.

3. The dietary therapy composition and preparation method for promoting the repair of damaged brain nerve cells according to claim 1, characterized in that: The artichoke peptide is an extract of artichoke receptacle, and the purity of the artichoke peptide is ≥90%. It is obtained by water extraction and compound enzymatic hydrolysis purification. The natural nicotinic acid is derived from quinoline acid in acerola cherry fruit powder.

4. The dietary therapy composition for promoting the repair of damaged brain nerve cells and its preparation method according to claim 3, characterized in that: The acerola cherry fruit powder is made from fresh acerola cherry fruit, which is washed, pitted, freeze-dried at -40 to -30℃, and pulverized. It contains natural quinolinic acid.

5. A dietary therapy composition for promoting the repair of damaged brain nerve cells and its preparation method, characterized in that, The dietary composition for promoting brain nerve repair containing artichoke peptides as described in any one of claims 1-4 comprises the following steps: S1. Raw material pretreatment: Each raw material in the composition except for inulin is subjected to impurity removal treatment and then dried to obtain pretreated raw materials; S2. Nanoparticle pulverization: After the pretreated raw materials are mixed evenly, they are put into a nano airflow pulverizer for pulverization to obtain nano-scale mixed powder. S3. Low-temperature drying: The nano-grade mixed powder is dried at low temperature using a gradient heating method to remove residual moisture from the raw materials. Inert gas is used for protection during the drying process. S4. Inulin encapsulation: The dried mixed powder is mixed with inulin and encapsulated using a spray drying process to form encapsulated particles; S5. Mixing and Molding: After mixing the embedded particles, they are processed into finished products using molding equipment.

6. The dietary therapy composition for promoting the repair of damaged brain nerve cells and its preparation method according to claim 5, characterized in that: In step S1, the impurity removal process uses a combination of screening and magnetic separation to remove mechanical and magnetic impurities from the raw materials. The drying process is carried out at a temperature of 40-45℃ for 1-2 hours to ensure that the moisture content of the raw materials is ≤5%.

7. The dietary therapy composition for promoting the repair of damaged brain nerve cells and its preparation method according to claim 5, characterized in that: In S2, the nano airflow pulverizer is of type KQW. During the pulverization process, the working pressure of the equipment is 0.7-0.85MPa and the working power is 1600kW. The particle size of the nano-scale mixed powder after pulverization is 100-300nm, and the particle size distribution uniformity deviation is ≤15%.

8. The dietary therapy composition for promoting the repair of damaged brain nerve cells and its preparation method according to claim 5, characterized in that: In S3, the low-temperature drying process adopts a gradient heating method, with an initial temperature of 48-52℃, gradually increasing to 53-57℃, and a total drying time of 2-4 hours; In step S3, the initial temperature of the gradient heating is 48-52℃, which is gradually increased to 53-57℃, and the total drying time is 2-4 hours.

9. The dietary therapy composition for promoting the repair of damaged brain nerve cells and its preparation method according to claim 5, characterized in that: In S4, the inlet temperature of the spray drying process is 120-140℃, the outlet temperature is 60-70℃, the atomization pressure is 0.3-0.5MPa, and the particle size of the embedded particles is 80-120nm.

10. The dietary therapy composition for promoting the repair of damaged brain nerve cells according to claim 5 and its preparation method, characterized in that: In step S5, the mixing process uses a horizontal mixer with a mixing speed of 300-500 r / min, a mixing time of 15-30 minutes, and a mixing uniformity of ≥95%. The forming equipment is a tablet press or a granulator, which processes the product into tablets or granules. The disintegration time of the finished product is 15-30 minutes.

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