Long-acting soothing and moisturizing skin bottom essence and preparation method thereof

Abstract

The invention relates to the technical field of cosmetics, and particularly discloses long-acting soothing and moisturizing skin bottom essence and a preparation method thereof. The invention relates to a long-acting soothing and moisturizing skin bottom essence, which is prepared from the following raw materials in parts by weight: 90 to 95 parts of water, 3.5 to 4.5 parts of 1, 2-pentanediol, 0.8 to 1.2 parts of squalane, 0.6 to 0.68 part of hydrogenated lecithin, 0.15 to 0.2 part of phytosterols, 0.4 to 0.5 part of ammonium acryloyldimethyl taurate / VP copolymer, 0.2 to 0.3 part of sodium hyaluronate, 0.15 to 0.25 part of panthenol, 0.04 to 0.06 part of ceramide NP and 0.8 to 1.2 parts of deep sea barophilic bacteria fermentation extracting solution. The effective soothing and moisturizing skin bottom essence disclosed by the invention is relatively good in improvement on consideration of excellent corrosion resistance and low irritation.

Description

Technical Field

[0001] This application relates to the field of cosmetic technology, and more specifically, to a long-lasting soothing and moisturizing skin essence and its preparation method. Background Technology

[0002] The skin-before-serum is a brand-new hydrating and moisturizing product that can effectively improve skin condition, making the entire face lighter and smoother, more radiant and elastic, more effectively repairing the skin, and also fighting aging. It helps improve skin texture and makes the skin look brighter and more hydrated.

[0003] The core functions of this long-lasting soothing and moisturizing skin-prepping serum are deep repair, long-lasting hydration, and soothing and stabilizing. Among them, the repairing and soothing ingredients can relieve sensitivity symptoms such as redness and itching, and strengthen the skin barrier; the long-lasting moisturizing ingredients can improve dryness and peeling, and enhance the water retention capacity of the stratum corneum; at the same time, as a skincare base, this long-lasting soothing and moisturizing skin-prepping serum can also soften the stratum corneum, providing a channel for the absorption of subsequent serums or creams.

[0004] Regarding the aforementioned technologies, the inventors believe that long-lasting soothing and moisturizing skin essences contain high concentrations of active ingredients and are prone to microbial growth under high moisturizing conditions. Therefore, higher doses of preservatives are needed to inhibit contamination. However, many common preservatives contain phenols and hydroxyl compounds, which can irritate and cause allergic reactions to the skin, leading to adverse reactions such as dryness, itching, and redness. Therefore, it is difficult to achieve both excellent preservative properties and low irritation. Currently, there is an urgent need to propose a solution to address the aforementioned technical problems. Summary of the Invention

[0005] In order to achieve a balance between excellent anti-corrosion properties and low irritation, this application provides a long-lasting soothing and moisturizing skin essence and its preparation method.

[0006] In the first aspect, this application provides a long-lasting soothing and moisturizing skin essence, employing the following technical solution: A long-lasting soothing and moisturizing serum, made from the following ingredients in parts by weight: 90-95 parts water; 3.5-4.5 parts of 1,2-pentanediol; Squalane 0.8-1.2 parts; Hydrogenated lecithin 0.6-0.68 parts; Phytosterols: 0.15-0.2 parts; 0.4-0.5 parts of ammonium acryloyldimethyl taurate / VP copolymer; Sodium hyaluronate 0.2-0.3 parts; Panthenol 0.15-0.25 parts; Ceramide NP 0.04-0.06 parts; 0.8-1.2 parts of fermentation extract of deep-sea barotropic bacteria; The deep-sea barophilic bacteria fermentation extract was prepared through the following steps: S1. Using deep-sea barophilic bacteria in combination with culture medium, batch feeding fermentation was carried out under low temperature of 13-17℃ and high hydrostatic pressure of 40-60MPa. After fermentation, the fermentation broth was obtained. S2. Take the fermentation broth obtained in step S1 and mix it with a compound enzyme for an enzyme reaction. After the enzyme reaction, use a step-by-step depressurization treatment. Finally, add hexadecyltrimethylammonium bromide for precipitation and filter to obtain the deep-sea barophilic bacteria fermentation extract. The culture medium used consists of raw materials including trehalose, sodium pyruvate, yeast extract, peptone, ammonium nitrate, strontium chloride, sodium silicate, HEPES buffer and ultrapure water; The complex enzyme used consists of β-1,3-glucanase and proteinase K; The deep-sea barophilic bacteria used are derived from the gray algae *Exiguobacterium profundum* HZB115760.

[0007] By employing the above-mentioned technical solution, in the preparation of deep-sea barotropic bacteria fermentation extract, the low temperature of 13-17℃ can inhibit conventional microbial contamination and induce the expression of cold-adaptive enzymes in deep-sea barotropic bacteria, enhancing the stability of their metabolites. Meanwhile, the high hydrostatic pressure of 40-60 MPa can stimulate the secretion of pressure-resistant enzymes and extracellular polysaccharides. In addition to its own corresponding functions, the culture medium used can also increase the concentration and stability of active substances in the fermentation broth through strontium chloride and sodium silicate. Next, the fermentation broth is combined with a complex enzyme for enzymatic reactions. β-1,3-glucanase specifically cleaves β-glucan in the cell wall, releasing intracellular active substances. Proteinase K is used to degrade non-specific proteins, improving the purity of the target product. After the enzymatic reaction, a stepwise depressurization treatment is used to gradually relieve pressure-induced protein conformational changes, preventing irreversible inactivation of active ingredients and promoting the separation of cell debris and the target product, thus improving subsequent precipitation efficiency. Finally, hexadecyltrimethylammonium bromide precipitation is used to selectively precipitate negatively charged active ingredients and remove impurity proteins, yielding the deep-sea barotropic bacteria fermentation extract. Applying the fermentation extract of deep-sea pressure-loving bacteria to the aforementioned long-lasting soothing and moisturizing skin essence not only allows it to exert excellent antibacterial and preservative effects on its own, but also enables it to form a "bio-chemical" composite preservative system with 1,2-pentanediol, resulting in a synergistic effect. Furthermore, under this system, the overall irritation of the long-lasting soothing and moisturizing skin essence is lower, making it very suitable for sensitive skin. This achieves a balance between excellent preservative properties and low irritation in the product.

[0008] Preferably, the culture medium consists of raw materials comprising the following weight percentages: Trehalose 0.45-0.55%; Sodium pyruvate 0.15-0.25%; Yeast extract 0.08-0.12%; Peptone 0.04-0.06%; Ammonium nitrate 0.008-0.012%; Strontium chloride 0.008-0.012%; Sodium silicate 0.004-0.006%; Adjust the pH of the culture medium to 7.5-8.0 using HEPES buffer; Ultrapure water balance.

[0009] By adopting the above technical solutions, trehalose, as the main carbon source, has a more stable non-reducing structure under high pressure; sodium pyruvate can directly supplement energy metabolism precursors and can replace part of the glycolysis function under high pressure, alleviating energy metabolism pressure; yeast extract provides B vitamins, nucleotides and other growth factors to promote enzyme system activation under high pressure; peptone provides small peptides and free amino acids to meet the needs of rapid protein synthesis under extreme environments; ammonium nitrate, as an inorganic nitrogen source supplement, forms a synergistic effect with organic nitrogen sources; strontium chloride and sodium silicate can provide stabilization and protection; HEPES buffer can play an excellent buffering and pH control role; and the culture medium obtained by combining the above raw materials with ultrapure water can be adapted to the extreme environment of deep-sea barophilic bacteria.

[0010] Preferably, in step S2, after the fermentation broth and the complex enzyme are mixed, the concentrations of β-1,3-glucanase and proteinase K are each 0.01%.

[0011] By employing the above technical solution, at the aforementioned concentration, β-1,3-glucanase can efficiently cleave β-1,3-glucan in the cell wall of barotrophic bacteria, releasing intracellular active substances. Meanwhile, proteinase K can degrade high-pressure denatured proteins and non-target proteins, while avoiding damage to pressure-resistant enzymes. Furthermore, the synergistic effect of both can completely decompose the cell wall-protein complex, improving the purity of active ingredients in the extract. Therefore, this concentration combination achieves efficient release and complete retention of target components by precisely controlling the enzymatic hydrolysis intensity.

[0012] Preferably, in step S2, the enzyme reaction temperature is 18-22℃, the pH is adjusted to 7-8, and the reaction is stirred for 1.8-2.2 hours.

[0013] By adopting the above technical solution, a reaction temperature of 18-22℃ can reduce the risk of enzyme self-hydrolysis and maintain the high stability of the complex enzyme; adjusting the pH to 7-8 can ensure the selective hydrolysis of denatured proteins by proteinase K, while avoiding the inactivation of the target enzyme due to extreme pH; stirring the reaction for 1.8-2.2h can ensure sufficient enzymatic hydrolysis; therefore, the combination of the above parameters can precisely control the enzymatic hydrolysis kinetics, thereby bringing excellent enzymatic hydrolysis effect.

[0014] Preferably, in step S2, the step-by-step pressure reduction process involves reducing the pressure from 50 MPa to 30 MPa in the high-pressure reactor, maintaining it for 12-18 minutes, then reducing it to 10 MPa, maintaining it for 12-18 minutes, and finally reducing it to atmospheric pressure of 0.1 MPa.

[0015] By employing the above technical solution, the cell membranes of deep-sea barotropic bacteria are rich in unsaturated fatty acids and pressure-adaptive proteins. When the pressure is reduced from 50 to 30 MPa, cell rupture caused by membrane lipid phase transition can be avoided, maintaining the integrity of the enzyme reaction system. Moreover, the pressure gradient change activates the mechanosensitive channels of bacteria, promoting the orderly release of intracellular active substances. In the stage of reducing pressure from 30 to 10 MPa, the pressure reduction causes the enzyme molecule conformation to slowly unfold, avoiding the inactivation of active sites caused by drastic pressure reduction. The selected holding time ensures the stable dissociation of the enzyme-substrate complex. When the pressure is finally reduced to normal, the microbubbles generated by the sudden pressure drop disrupt the binding of cell debris and target products, without creating a homogeneous solution environment for subsequent CTAB precipitation, thus reducing non-specific precipitation. Therefore, through the above pressure-time coupling control in the stepwise depressurization process, a triple synergistic effect of cell protection, enzyme activity stabilization, and product purification is achieved, which is conducive to obtaining a deep-sea barotropic bacteria fermentation extract with better application quality.

[0016] Preferably, the raw materials also contain 0.08-0.12 parts by weight of a reinforcing composite additive, which is composed of soluble collagen, mannitol and trehalose, and the weight ratio of soluble collagen, mannitol and trehalose is (4-6):(5-10):1.

[0017] By adopting the above technical solutions, soluble collagen can form a breathable polymer membrane in application, blocking microorganisms from contacting external irritants and maintaining normal skin respiration; mannitol enhances the preservative effect by reducing water activity, inhibiting the reproduction of bacteria and fungi; trehalose, as a natural preservative, can stabilize the structure of active ingredients in extreme environments and extend the shelf life of the preservative system; when soluble collagen, mannitol, and trehalose are used as a reinforcing composite adjuvant, the three work synergistically to form a composite membrane structure, which not only significantly enhances the preservative performance but also exerts excellent osmotic pressure regulation, maintains cell homeostasis, and significantly reduces irritation, thereby further improving the preservative and low-irritant properties of the long-lasting soothing and moisturizing skin essence.

[0018] Preferably, the weight ratio of the soluble collagen, mannitol and trehalose is 5:8:1.

[0019] By adopting the above technical solution, the soluble collagen, mannitol and trehalose in the above proportions have better synergistic effects when applied, which can bring better results in improving the quality of long-lasting soothing and moisturizing skin essence.

[0020] Secondly, this application provides a method for preparing a long-lasting soothing and moisturizing skin essence, using the following technical solution: A method for preparing a long-lasting soothing and moisturizing skin base essence includes the following steps: (1) Prepare the raw materials according to the ratio, including water, 1,2-pentanediol, squalane, hydrogenated lecithin, phytosterols, ammonium acryloyldimethyl taurate / VP copolymer, sodium hyaluronate, panthenol, ceramide NP and deep-sea pressure-loving bacteria fermentation extract; (2) The water, sodium hyaluronate and panthenol in step (1) are heated and mixed to obtain the first composition; the 1,2-pentanediol, squalane, hydrogenated lecithin and phytosterols in step (1) are heated and mixed to obtain the second composition; (3) Mix the first composition and the second composition obtained in step (2), stir homogenize and cool down, then add ammonium acryloyl dimethyl taurate / VP copolymer and mix and disperse, and finally add ceramide NP and deep-sea pressure-loving bacteria fermentation extract and mix and disperse to obtain long-lasting soothing and moisturizing skin essence.

[0021] By adopting the above technical solution, the above operation is relatively simple and suitable for large-scale industrial production. At the same time, the addition and use of each raw material in stages not only facilitates quality control during the process, but also enables the raw materials to fully cooperate, which is conducive to obtaining a long-lasting soothing and moisturizing skin essence with excellent and stable application quality.

[0022] In summary, this application has the following beneficial effects: 1. This application uses a specially prepared deep-sea pressure-loving bacteria fermentation extract and 1,2-pentanediol to form a "bio-chemical" composite preservative system, which exerts an excellent compound synergistic effect, thereby improving the excellent preservative properties and low irritation of the effective soothing and moisturizing skin essence. 2. This application uses soluble collagen, mannitol and trehalose as a reinforcing compound additive. The three are synergistically combined to form a composite membrane structure, which not only significantly enhances the antiseptic performance, but also plays an excellent role in osmotic pressure regulation, maintaining cell homeostasis, and significantly reducing the impact of irritation. This further improves the antiseptic and low-irritation properties of the long-lasting soothing and moisturizing skin essence. Detailed Implementation

[0023] The present application will be further described in detail below with reference to preparation examples, embodiments and comparative examples.

[0024] Unless otherwise specified, all raw materials used in the preparation examples, embodiments and comparative examples of this application are commercially available.

[0025] The deep-sea barophilic bacteria used were derived from the gray algae *Exiguobacterium profundum* HZB115760.

[0026] Preparation of raw materials and / or intermediates Example 1 A fermentation extract of deep-sea barophilic bacteria is prepared by the following steps: S1. Using deep-sea barophilic bacteria in a culture medium with an inoculum size of 8%, the fermentation was carried out in batches at a low temperature of 13-17℃ and a high hydrostatic pressure of 40-60MPa. When the dissolved oxygen (DO) was greater than 30%, the culture medium was added. After 60 days of continuous fermentation, the fermentation broth was obtained. S2. Take the fermentation broth obtained in step S1 and mix it with a compound enzyme for an enzymatic reaction. After the enzymatic reaction, use a stepwise depressurization treatment. Finally, heat 2% of the mixture with hexadecyltrimethylammonium bromide and stir to precipitate. After standing for 24 hours, filter to obtain the fermentation extract of deep-sea pressure-loving bacteria.

[0027] Note: In step S1 of this embodiment, the preferred conditions are a low temperature of 15°C and a high hydrostatic pressure of 50 MPa. Table 1 shows the composition of the culture medium and its corresponding weight percentages. The pH of the culture medium is adjusted to 7.5-8.0 using HEPES buffer; in this embodiment, the pH is 8.0. The complex enzyme used consists of β-1,3-glucanase and proteinase K, and after mixing the fermentation broth and the complex enzyme, the concentrations of β-1,3-glucanase and proteinase K are each 0.01%. In step S2, the enzyme reaction temperature is 80°C, the pH is adjusted to 7.5, and the reaction is stirred for 2 hours. The stepwise depressurization process involves reducing the pressure in the high-pressure reactor from 50 MPa to 30 MPa, holding for 15 minutes, then reducing it to 10 MPa, holding for 15 minutes, and finally reducing it to atmospheric pressure (0.1 MPa).

[0028] Preparation Examples 2-3 A fermentation extract of deep-sea pressure-loving bacteria, which differs from Preparation Example 1 in that the composition of the culture medium and its corresponding weight percentages are shown in Table 1.

[0029] Table 1. Composition of the culture media in Examples 1-3 and their corresponding weight percentages (%) raw material Preparation Example 1 Preparation Example 2 Preparation Example 3 Trehalose 0.50 0.45 0.55 Sodium pyruvate 0.20 0.25 0.15 Yeast extract 0.10 0.08 0.12 peptone 0.05 0.06 0.04 ammonium nitrate 0.010 0.008 0.012 Strontium chloride 0.010 0.012 0.008 Sodium silicate 0.005 0.004 0.006 Ultrapure water margin margin margin Preparation Example 4 A fermentation extract of deep-sea pressure-loving bacteria, which differs from Preparation Example 1 in that, in step S2, the enzyme reaction temperature is 18°C, the pH is adjusted to 7, and the reaction is stirred for 2.2 hours.

[0030] Preparation Example 5 A fermentation extract of deep-sea pressure-loving bacteria, which differs from preparation example 1, is prepared in step S2, where the enzyme reaction temperature is 22°C, the pH is adjusted to 8, and the reaction is stirred for 1.8 hours.

[0031] Preparation Example 6 A deep-sea pressure-loving bacteria fermentation extract differs from Preparation Example 1 in that, in step S2, the step-by-step depressurization process involves reducing the pressure from 50 MPa to 30 MPa in a high-pressure reactor, maintaining this pressure for 12 minutes, then reducing it to 10 MPa, maintaining this pressure for 12 minutes, and finally reducing it to atmospheric pressure of 0.1 MPa.

[0032] Preparation Example 7 A deep-sea pressure-loving bacteria fermentation extract differs from Preparation Example 1 in that, in step S2, the step-by-step depressurization process involves reducing the pressure from 50 MPa to 30 MPa in a high-pressure reactor, maintaining this pressure for 18 minutes, then reducing it to 10 MPa, maintaining this pressure for 18 minutes, and finally reducing it to atmospheric pressure of 0.1 MPa. Example

[0033] Example 1 A long-lasting soothing and moisturizing skin essence, the raw materials used in its preparation and their corresponding weight parts are shown in Table 2, and it is prepared through the following steps: (1) Prepare the raw materials according to the ratio, including water, 1,2-pentanediol, squalane, hydrogenated lecithin, phytosterols, ammonium acryloyldimethyl taurate / VP copolymer, sodium hyaluronate, panthenol, ceramide NP and deep-sea pressure-loving bacteria fermentation extract; (2) The water, sodium hyaluronate and panthenol in step (1) are heated to 70°C and mixed to obtain the first composition; the 1,2-pentanediol, squalane, hydrogenated lecithin and phytosterols in step (1) are heated to 70°C and mixed to obtain the second composition; (3) Mix the first composition and the second composition obtained in step (2), stir homogenously and cool to 40°C, then add ammonium acryloyl dimethyl taurate / VP copolymer and mix and disperse, and finally cool to 25°C and add ceramide NP and deep-sea pressure-loving bacteria fermentation extract and mix and disperse to obtain long-lasting soothing and moisturizing skin essence.

[0034] Note: The deep-sea pressure-loving bacteria fermentation extract used in the above operations was obtained from Preparation Example 1.

[0035] Example 2-3 A long-lasting soothing and moisturizing skin essence, which differs from Example 1 in that the raw materials used in its preparation and their corresponding weight parts are shown in Table 2.

[0036] Table 2. Raw materials used in the preparation of Examples 1-3 and their corresponding weight parts (parts / kg) Example 4 A long-lasting soothing and moisturizing skin essence, which differs from Example 1 in that the deep-sea pressure-loving bacteria fermentation extract used is obtained from Preparation Example 2.

[0037] Example 5 A long-lasting soothing and moisturizing skin essence, which differs from Example 1 in that the deep-sea pressure-loving bacteria fermentation extract used is obtained from Preparation Example 3.

[0038] Example 6 A long-lasting soothing and moisturizing skin essence, which differs from Example 1 in that the deep-sea pressure-loving bacteria fermentation extract used is obtained from Preparation Example 4.

[0039] Example 7 A long-lasting soothing and moisturizing skin essence, which differs from Example 1 in that the deep-sea pressure-loving bacteria fermentation extract used is obtained from Preparation Example 5.

[0040] Example 8 A long-lasting soothing and moisturizing skin essence, which differs from Example 1 in that the deep-sea pressure-loving bacteria fermentation extract used is obtained from Preparation Example 6.

[0041] Example 9 A long-lasting soothing and moisturizing skin essence, which differs from Example 1 in that the deep-sea pressure-loving bacteria fermentation extract used is obtained from Preparation Example 7.

[0042] Example 10 A long-lasting soothing and moisturizing skin essence, which differs from Example 1 in that it also contains 0.1 parts by weight of a strengthening complex agent in the raw materials. The strengthening complex agent is composed of soluble collagen, mannitol and trehalose in a weight ratio of 5:8:1. When the strengthening complex agent is applied, step (2) is specifically set as follows: water, sodium hyaluronate, panthenol and strengthening complex agent in step (1) are heated to 70°C and mixed to obtain the first composition; 1,2-pentanediol, squalane, hydrogenated lecithin and phytosterols in step (1) are heated to 70°C and mixed to obtain the second composition.

[0043] Example 11 A long-lasting soothing and moisturizing skin essence, which differs from Example 10 in that the amount of the enhanced complex additive added is 0.08 parts by weight.

[0044] Example 12 A long-lasting soothing and moisturizing skin essence, which differs from Example 10 in that the amount of the enhanced complex additive added is 0.12 parts by weight.

[0045] Example 13 A long-lasting soothing and moisturizing skin essence, which differs from Example 10 in that the strengthening complex is composed of soluble collagen, mannitol and trehalose in a weight ratio of 5:7.5:1.

[0046] Example 14 A long-lasting soothing and moisturizing skin essence, which differs from Example 10 in that the strengthening complex is composed of soluble collagen, mannitol and trehalose in a weight ratio of 4:5:1.

[0047] Example 15 A long-lasting soothing and moisturizing skin essence, which differs from Example 10 in that the strengthening complex is composed of soluble collagen, mannitol and trehalose in a weight ratio of 6:10:1.

[0048] Example 16 A long-lasting soothing and moisturizing skin essence, which differs from Example 10 in that it does not use soluble collagen or mannitol in its ingredients.

[0049] Example 17 A long-lasting soothing and moisturizing skin essence, which differs from Example 10 in that it does not use soluble collagen or trehalose in its ingredients.

[0050] Example 18 A long-lasting soothing and moisturizing skin essence, which differs from Example 10 in that mannitol and trehalose are not used in the ingredients.

[0051] Example 19 A long-lasting soothing and moisturizing skin essence, which differs from Example 10 in that soluble collagen is not used in the ingredients.

[0052] Example 20 A long-lasting soothing and moisturizing skin essence, which differs from Example 10 in that mannitol is not used in the ingredients.

[0053] Example 21 A long-lasting soothing and moisturizing skin essence, which differs from Example 10 in that trehalose is not used in the ingredients.

[0054] Comparative Example Comparative Example 1 A long-lasting soothing and moisturizing skin essence, which differs from Example 1 in that it does not use deep-sea pressure-loving bacteria fermentation extract in its raw materials.

[0055] Comparative Example 2 A long-lasting soothing and moisturizing skin essence, which differs from Example 1 in that 1,2-pentanediol is not used in the ingredients.

[0056] Comparative Example 3 A long-lasting soothing and moisturizing skin essence, which differs from Example 1 in that it does not use deep-sea pressure-loving bacteria fermentation extract and 1,2-pentanediol in its raw materials.

[0057] Performance testing test samples: The long-lasting soothing and moisturizing skin essence obtained in Examples 1-21 was selected as test sample 1-21, and the long-lasting soothing and moisturizing skin essence obtained in Comparative Examples 1-3 was selected as control sample 1-3.

[0058] Test methods: (1) Antiseptic performance test: Commonly used strains Staphylococcus aureus (ATCC 6538), Pseudomonas aeruginosa (ATCC 15442), and Candida albicans (ATCC 10231) were selected to prepare bacterial suspensions with a concentration of 10. 5 -10 6 CFU / g, then the bacterial solution was added to the long-lasting soothing and moisturizing skin essence sample (addition amount 20g / 20mL), mixed evenly and incubated in an environment of 22.5℃±2.5℃ for 3 days. The plate count method was used to calculate the colony count reduction rate of the above strains, and the average value of the three strains was recorded as the final value. (2) Irritation test: SPF-grade white Laihang chicken fertilized embryos, weighing 55g, were used; incubation conditions: temperature 37.5±0.5℃, humidity 60%, incubation until day 9; 0.3mL of long-lasting soothing and moisturizing skin essence was added to the embryo using a pipette and the time was started. The vascular reaction was observed after five minutes, and the severity of the following phenomena was scored, with the score accurate to 0.1: Bleeding is scored from 1 to 3 (inclusive), with blood flowing out of the blood vessels, manifesting as punctate or flocculent hemorrhages; The coagulation score is 3-6 (inclusive), indicating protein denaturation in blood vessels, slowed blood flow or thrombosis, and the blood vessels appear brownish-black. The rate of vascular dissolution is 6-10 points, indicating that the walls of small blood vessels rupture and the blood vessels disappear.

[0059] After performing the above tests on test samples 1-21 and control samples 1-3, the test results are recorded in Table 3.

[0060] Table 3. Test results of test samples 1-21 and control samples 1-3 Combining Example 1 and Comparative Examples 1-3 with Table 3, it can be seen that the combination of specially prepared deep-sea barotropic bacteria fermentation extract and 1,2-pentanediol significantly improves the preservative and low-irritant properties of the long-lasting soothing and moisturizing skin essence. While either the deep-sea barotropic bacteria fermentation extract or 1,2-pentanediol alone can improve the effect, the improvement is limited. The sum of the improvement effects of using either one alone is far less than that of the combination. Therefore, it can be seen that the deep-sea barotropic bacteria fermentation extract and 1,2-pentanediol can exert a significant improvement effect of 1+1>2 in the long-lasting soothing and moisturizing skin essence.

[0061] As can be seen from Examples 1 and 10-15, and in conjunction with Table 3, when soluble collagen, mannitol, and trehalose are used as a reinforcing compound in this application, the preservative and low-irritant properties of the long-lasting soothing and moisturizing skin essence are further enhanced. Furthermore, as can be seen from Examples 16-21 and Table 3, while combining any one or two of soluble collagen, mannitol, and trehalose can improve the effect, the improvement is limited, and the effect is only a simple additive one, far less effective than the combined effect of all three.

[0062] This specific embodiment is merely an explanation of this application and is not intended to limit it. After reading this specification, those skilled in the art can make modifications to this embodiment without contributing any inventive step, but such modifications are protected by patent law as long as they fall within the scope of the claims of this application.

Claims

1. A long-lasting soothing and moisturizing skin essence, characterized in that, Made from the following ingredients in parts by weight: 90-95 parts water; 3.5-4.5 parts of 1,2-pentanediol; Squalane 0.8-1.2 parts; Hydrogenated lecithin 0.6-0.68 parts; Phytosterols: 0.15-0.2 parts; 0.4-0.5 parts of ammonium acryloyldimethyl taurate / VP copolymer; Sodium hyaluronate 0.2-0.3 parts; Panthenol 0.15-0.25 parts; Ceramide NP 0.04-0.06 parts; 0.8-1.2 parts of fermentation extract of deep-sea barotropic bacteria; The deep-sea barophilic bacteria fermentation extract was prepared through the following steps: S1. Using deep-sea barophilic bacteria in combination with culture medium, batch feeding fermentation was carried out under low temperature of 13-17℃ and high hydrostatic pressure of 40-60MPa. After fermentation, the fermentation broth was obtained. S2. Take the fermentation broth obtained in step S1 and mix it with a compound enzyme for an enzyme reaction. After the enzyme reaction, use a step-by-step depressurization treatment. Finally, add hexadecyltrimethylammonium bromide for precipitation and filter to obtain the deep-sea barophilic bacteria fermentation extract. The culture medium used consists of raw materials including trehalose, sodium pyruvate, yeast extract, peptone, ammonium nitrate, strontium chloride, sodium silicate, HEPES buffer and ultrapure water; The complex enzyme used consists of β-1,3-glucanase and proteinase K; The deep-sea barophilic bacteria used are derived from the gray algae *Exiguobacterium profundum* HZB115760.

2. The long-lasting soothing and moisturizing skin essence according to claim 1, characterized in that: The culture medium consists of raw materials comprising the following weight percentages: Trehalose 0.45-0.55%; Sodium pyruvate 0.15-0.25%; Yeast extract 0.08-0.12%; Peptone content: 0.04-0.06%; Ammonium nitrate 0.008-0.012%; Strontium chloride 0.008-0.012%; Sodium silicate 0.004-0.006%; Adjust the pH of the culture medium to 7.5-8.0 using HEPES buffer; Ultrapure water balance.

3. The long-lasting soothing and moisturizing skin essence according to claim 1, characterized in that: In step S2, after the fermentation broth and the complex enzyme are mixed, the concentrations of β-1,3-glucanase and proteinase K are each 0.01%.

4. The long-lasting soothing and moisturizing skin essence according to claim 1, characterized in that: In step S2, the enzyme reaction temperature is 18-22℃, the pH is adjusted to 7-8, and the reaction is stirred for 1.8-2.2 hours.

5. The long-lasting soothing and moisturizing skin essence according to claim 1, characterized in that: In step S2, the step-by-step depressurization process involves reducing the pressure from 50 MPa to 30 MPa in the high-pressure reactor, maintaining this pressure for 12-18 minutes, then reducing it to 10 MPa, maintaining this pressure for 12-18 minutes, and finally reducing it to atmospheric pressure of 0.1 MPa.

6. The long-lasting soothing and moisturizing skin essence according to claim 1, characterized in that: The raw materials also contain 0.08-0.12 parts by weight of a reinforcing composite additive, which is composed of soluble collagen, mannitol and trehalose, and the weight ratio of soluble collagen, mannitol and trehalose is (4-6):(5-10):

1.

7. The long-lasting soothing and moisturizing skin essence according to claim 6, characterized in that: The weight ratio of the soluble collagen, mannitol, and trehalose is 5:8:

1.

8. The method for preparing the long-lasting soothing and moisturizing skin essence according to claim 1, characterized in that: Includes the following steps: (1) Prepare the raw materials according to the proportions, including water, 1,2-pentanediol, squalane, hydrogenated lecithin, phytosterols, ammonium acryloyldimethyl taurate / VP copolymer, sodium hyaluronate, panthenol, ceramide NP and deep-sea pressure-loving bacteria fermentation extract; (2) The water, sodium hyaluronate and panthenol in step (1) are heated and mixed to obtain the first composition; the 1,2-pentanediol, squalane, hydrogenated lecithin and phytosterols in step (1) are heated and mixed to obtain the second composition; (3) Mix the first composition and the second composition obtained in step (2), stir homogenize and cool down, then add ammonium acryloyl dimethyl taurate / VP copolymer and mix and disperse, and finally add ceramide NP and deep-sea pressure-loving bacteria fermentation extract and mix and disperse to obtain long-lasting soothing and moisturizing skin essence.

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