Insecticidal composition containing chlorfenapyr and application thereof

Abstract

The invention belongs to the technical field of pesticide disinfestation, and discloses a chlorfenapyr-containing insecticidal composition and application thereof.The active ingredients of the insecticidal composition comprise chlorfenapyr and a compound shown in the formula I. The structure of the compound shown in the formula I is as follows: the mass ratio of the compound shown in the formula I to chlorfenapyr is 1: 40-35: 1. The insecticidal composition disclosed by the invention can be used for effectively preventing and treating pests such as lepidoptera and thysanoptera, especially has a remarkable effect on preventing beet armyworms, plutella xylostella, cabbage caterpillars and thrips, is high in safety to crops and does not generate phytotoxicity.

Description

Technical Field

[0001] This invention belongs to the field of pesticide insecticide technology, and discloses an insecticidal composition containing chlorfenapyr and its application. Background Technology

[0002] Chlorfenapyr, also known as bromfenac, is an arylpyrrole insecticide and acaricide. It mainly acts as a stomach poison and has some contact activity. It is commonly used to control pests and mites on cotton, vegetables, citrus, fruit, vines, and soybeans.

[0003] Compound I is a novel compound independently developed by our company. This compound has a broad insecticidal spectrum, low toxicity, and high efficacy. The structure of compound I is shown below:

[0004]

[0005] Currently, pest control primarily relies on the use of insecticides. In practice, selecting appropriate pesticides and using them rationally is crucial to achieving both pest control and reduced environmental pollution. The applicant, using the commonly used insecticide chlorfenapyr as a base, compounded Formula I with chlorfenapyr. Through indoor screening, the synergistic effect against various pests was determined, and field efficacy trials were conducted. The control effects of the compounded pesticide on diamondback moth, beet armyworm, and thrips were investigated, aiming to identify highly effective and economical insecticide compound formulations to provide a reference for pest control in the field. Summary of the Invention

[0006] To address the aforementioned problems in the prior art, this invention provides an insecticidal composition containing chlorfenapyr and its application. This insecticidal composition effectively controls pests such as Lepidoptera and Thysanoptera, improves pest control efficacy, slows the development of pesticide resistance in pests, and reduces environmental pollution.

[0007] To achieve the above objectives, the present invention adopts the following technical solution: an insecticidal composition containing chlorfenapyr, wherein the active ingredient of the insecticidal composition comprises chlorfenapyr and a compound of formula I, the structure of which is as follows: The mass ratio of the compound of formula I to chlorfenapyr is 1:40 to 35:1, or any value within the above range.

[0008] Furthermore, the mass ratio of the compound of formula I to chlorfenapyr is 1:36 to 35:1, or any value within the above range.

[0009] Furthermore, the mass ratio of the compound of formula I to chlorfenapyr is 1:30 to 32:1, or any value within the above range;

[0010] Furthermore, the mass ratio of the compound of formula I to chlorfenapyr is 1:20 to 16:1, or any value within the above range.

[0011] Furthermore, in addition to the active ingredient, the insecticidal composition also includes pesticide-acceptable auxiliary ingredients, which are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, preservatives, stabilizers, synergists, or carriers.

[0012] The wetting agent is selected from one or more of the following: alkylbenzene sulfonate, alkylnaphthalene sulfonate, lignin sulfonate, sodium dodecyl sulfate, sodium dioctyl succinate sulfonate, α-olefin sulfonate, alkylphenol polyoxyethylene ether, castor oil polyoxyethylene ether, alkylphenol ethoxylate, fatty alcohol ethoxylate, sodium fatty alcohol polyoxyethylene ether sulfate, silkworm excrement, soapberry powder, soapberry powder, SOPA, detergent, emulsifier 2000 series, and wetting and penetrating agent F; and / or

[0013] The dispersant is selected from one or more of the following: lignin sulfonates, alkyl naphthalene sulfonates formaldehyde condensates, naphthalene sulfonates, tristyrylphenol ethoxylate phosphates, fatty alcohol ethoxylates, alkylphenol polyoxyethylene ethers, alkylphenol polyoxyethylene ether methyl ether condensates sulfates, fatty amine polyoxyethylene ethers, glycerol fatty acid ester polyoxyethylene ethers, polycarboxylates, polyacrylic acids, phosphates, EO-PO block copolymers, and EO-PO graft copolymers; and / or

[0014] The emulsifier is selected from one or more of the following: calcium dodecylbenzenesulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, phenethylphenol polyoxyethylene polyoxypropylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, styrene-phenol polyoxyethylene ether, castor oil polyoxyethylene ether, and alkylphenol ether phosphate; and / or

[0015] The thickener is selected from one or more of xanthan gum, organobentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose, and silica; and / or

[0016] The disintegrant is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid, and tartaric acid; and / or

[0017] Antifreeze is selected from one or more of alcohols, alcohol ethers, chlorinated hydrocarbons, and inorganic salts; and / or

[0018] Defoamer selected from C 10 -C 20 Saturated fatty acid compounds, silicone oil, silicone compounds, C8-C 10 One or more of the fatty alcohols; and / or

[0019] The solvent is selected from one or more of benzene, toluene, xylene, mesitylene, methanol, ethanol, isopropanol, n-butanol, dimethyl sulfoxide, dimethylformamide, cyclohexanone, hydrocarbon carbonates, diesel oil, solvent oil, vegetable oil, vegetable oil derivatives, and water; and / or

[0020] The preservative is selected from one or more of propionic acid, sodium propionate, sorbic acid, sodium sorbate, potassium sorbate, benzoic acid, sodium benzoate, sodium p-hydroxybenzoate, methyl p-hydroxybenzoate, Kathon, and 1,2-benzisothiazolin-3-one; and / or

[0021] The stabilizer is selected from one or more of the following: disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-cresol, triethanolamine oleate, epoxidized vegetable oil, kaolin, bentonite, attapulgite, silica, talc, montmorillonite, and starch; and / or

[0022] Synergists are selected from synergistic phosphorus, synergistic ether; and / or

[0023] The carrier is selected from one or more of the following: ammonium salts, ground natural minerals, ground artificial minerals, silicates, resins, waxes, solid fertilizers, water, organic solvents, mineral oils, vegetable oils, and vegetable oil derivatives.

[0024] Furthermore, based on a total weight of 100 wt%, the total weight of the chlorfenapyr and the compound of formula I accounts for 0.1% to 80% of the insecticidal composition;

[0025] Furthermore, the insecticidal composition is prepared into a pesticide-permitted formulation, wherein the formulation is a solid or liquid formulation;

[0026] Furthermore, the solid dosage forms include powders, granules, balls, tablets, strips, wettable powders, oil-dispersible powders, emulsion powders, water-dispersible granules, emulsion granules, water-dispersible tablets, soluble powders, soluble tablets, or soluble granules;

[0027] The liquid formulations include soluble agents, colloids, oils, spreading oils, emulsions, latexes, dispersible liquids, ointments, water emulsions, oil emulsions, microemulsions, lipid suspensions, microcapsule suspensions, oil suspensions, dispersible oil suspensions, suspensions, microcapsule suspension-suspension agents, microcapsule suspension-water emulsions, or microcapsule suspension-suspension emulsions.

[0028] Furthermore, the solid formulation is a wettable powder or a water-dispersible granule, and the liquid formulation is a microemulsion, an emulsion, or a suspension.

[0029] The present invention also discloses the application of the insecticidal composition described above for the control of plant pests;

[0030] Furthermore, the pests are Lepidoptera or Thysanoptera pests;

[0031] The Lepidoptera mentioned include, but are not limited to, the diamondback moth (Plutella xylostella), the small diamondback moth (Plutella xylostella), the grape berry moth (Polychrosis viteana), the citrus berry borer (Prays endocarpa), the olive borer (Prays oleae), species of Pseudaletia (Pseudaletia spp.) (noctus moth), Pseudaletia unipunctata (armyworm), the soybean looper (Pseudoplusia includes), the inchworm (Rachiplusia nu), the rice stem borer (Scirpophaga incertulas), species of Sesamia (Sesamia spp.) (stem borers), the pink rice stem borer (Sesamia inferens), and the rice stem borer (Sesamia inferens). The following species are listed: *Spodoptera nonagrioides*, *Setora nitens*, *Sitotroga cerealella* (Angoumois grain moth), *Sparganothis pilleriana*, *Spodoptera spp.* (noctuid moth), *Spodoptera exigua* (beet armyworm), *Spodoptera fugiperda* (fall armyworm), *Adoxophyes spp.*, *Adoxophyes orana*, and *Agrotis spp.*(Root-cutting worm), Agrotis ipsilon (black cutworm), Alabama argillacea, Amorbia cuneana, Amyelosis transitella (navel orange worm), Anarsia lineatella (peach twig borer), Anomis sabulifera (jute looper), Anticaria gemma talis (velvet beanca te r pillar), Archips argyrospila (fruittree leafroller), Archips rosana (roseleaf roller), Argyrotaenia spp. (tortricid moths), Argyrotaenia citrana, Autographa gamma, Bonagota *Cranaodes*, *Borbocinnara*, *Caloptilia* spp. (leaf miners), *Capua reticulana*, *Carposina niponensis* (peach fruit moth), *Chilo* spp., *Chlumetia transversa* (mango shoot borer), *Choristoneurarosaceana* (obliquebanded leafroller), *Chrysodeixis* spp., *Cnaphalocerus medinalis* (grass leafroller), *Colias* spp., *Conpomorpha cramerella*, *Cossus cossus*, *Crambus* spp.(Sod webworms), Plum fruit moth (Cydiafunebrana), Oriental fruit moth (Cydia molesta), Pea moth (Cydia nignicana), Apple leafroller (Cydia pomonella), Darnadiducta, Stem borers (Diaphania spp.), Stalk borers (Diatraea spp.), Sugarcane borer (Diatraea saccharalis), Southwest corn borer (Diatraea graniosella), Cotton bollworm (Earias spp.), Egyptian bollworm (Earias) *Egyptian bollworm* (*E. insulata*), *Rough northern bollworm* (*Earias vitella*), *Ecdytopopha aurantianum*, *Lesser cornstalk borer* (*Elasmopalpus lignosellus*), *Flour moths* (*Ephestia spp.*), *Almond moth* (*Ephestia cautella*), *Tobacco moth* (*Ephestia elutella*), *Mediterranean flour moth* (*Ephestia kuehniella*), *Epimeces spp.*, *Epinotia aporema*, *Banana skipper* (*Erionota thrax*), *Eupoecilia ambiguella*, *Euxoa* *Auxiliaris* (army cutworm), species of the genus *Feltia*, and species of the genus *Gortyna*.(Stemborers), Oriental fruit moth (Grapholita molesta), Hedyleptaindicate, bean leaf webber, noctuid moth (Helicoverpa spp.), cotton bollworm (Helicover paarmigera), cereal noctuid moth (Helicoverpa zea), noctuid moth (Heliothis spp.), tobacco bud noctuid moth (Heliothis virescens), tobacco budworm (Hellula undalis), cabbage webworm (Hellula undalis), root borers (Indarbela spp.), tomato codling moth (Keiferia lycopersicella), tomato pinworm, eggplant fruit borer (Leucinodes orbonalis). * *Borr*, *Leucopteramalifoliella*, *Lithocollectis* spp., *Lobesia botrana* (grape fruit moth), *Loxagrotis* spp. (noctus moth), *Loxagrotis albicosta* (western bean cutworm), *Lymantria dispar* (gypsy moth), *Lyonetia clerkella* (apple leaf miner), *Mahasena corbetti* (oil palm bagworm), and *Malacosoma* spp.(tent caterpillars), cabbage armyworm (Mamestra brassicae), bean pod borer (Maruca testulalis), bagworm (Metisa plana), true armyworm (Mythimna unipuncta), small tomato borer (Neoleucinodes elegantalis), winter moth (Operophtherabrumata), European corn borer (Ostrinia nubilalis), Pandemis cerasana, common currant tortrix, brown apple tortrix (Pandemis heparana), African swallowtail butterfly (Papilio demodocus), pink bollworm (Pectinophora gossypiella), species of Peridroma (Peridroma) spp. (root cutter), Peridroma saucia (variegated cutworm), Perileucoptera coffeella (white coffee leafminer), Phthorimaea operculella (potato tuber moth), Phyllocnisitis citrella (citrus leafminer), Phyllonorycter spp. (leaf miner), Pieris rapae (imported cabbage worm), Plathypenascabra (alfalfa green armyworm), Plodia interpunctella (Indian meal moth), Spodoptera oridania (southern armyworm), Synanthedon spp.(root borers), Thecla basilides, Thermisia gemmatalis, clothes moth (Tineola bisselliella) (webbing clothes moth), pink leafminer (Trichoplusiani) (cabbage worm), tomato leafminer (Tuta absoluta), nest moth (Yponomeuta spp.), coffee leopard borer (Zeuzera coffeae) (red branch borer), and pear leopard borer (Zeuzera pyrina) (leopard moth).

[0032] The aforementioned Thysanoptera pests include, but are not limited to: western flower thrips (Frankliniella occidentalis), thrips (Thrips spp.), yellow thrips (Scirtothrips dorsalis), rice thrips (Stenchaetothrips biformis), flower thrips (Frankliniella intonsta), palm thrips (Thrips palmi), yellow corn thrips (Anaphothrips obscurus), new spiny thrips (Neohydatothrips samayunkur), tea stick thrips (Dendrothrips minowai), rice tube thrips (Haplothrips aculeatus), grass thrips (Frankliniella tenuicornis), and yellow-breasted thrips (Thrips hawaiiensis);

[0033] In particular, the insecticidal composition of the present invention has excellent control effects on diamondback moth, beet armyworm, rice stem borer, cabbage caterpillar, and thrips.

[0034] Furthermore, the insecticidal composition or its formulation is applied to the pest that needs to be controlled or the medium in which it grows.

[0035] To achieve the desired insecticidal effect, the dosage of the insecticidal composition varies depending on various factors, such as the crop to be protected, the type of pest, the degree of infection, climatic conditions, application location, application method, and formulation used.

[0036] The beneficial effects of this invention are as follows:

[0037] 1) The insecticidal composition of the present invention rationally combines compounds with different mechanisms of action, and has a significant synergistic effect on Lepidoptera and Thysanoptera pests under appropriate mass ratios.

[0038] 2) The insecticidal composition of the present invention reduces the amount of pesticides used and slows down the development of pesticide resistance in pests. Detailed Implementation

[0039] To make the technical solution, objectives and advantages of the present invention clearer, the present invention is described with reference to the following specific embodiments. However, the present invention can be implemented in various forms and should not be limited to the embodiments described herein.

[0040] Formulation preparation example:

[0041] Preparation Example 1: 32% Compound I of Formula 1·chlorfenapyr water-dispersible granules (1:7)

[0042] Formula composition: 4% Formula I compound, 28% chlorfenapyr, 8% lignin sulfonate, 8% naphthalene sulfonate formaldehyde condensate D425, 2% sodium dodecyl sulfate, 5% silica, 25% starch, and kaolin to make up the balance.

[0043] Preparation method: According to the formulation ratio in the example, add the active ingredient to the carrier, and add surfactants and other functional additives therein, mix, and after air jet pulverization, add 10-25% water, and then knead, granulate, dry and sieve to obtain water-dispersible granules; or spray water, granulate and dry the pulverized powder in a fluidized bed granulator, and then sieve to obtain the product.

[0044] Preparation Example 2: 30% Formula I compound·chlorfenapyr wettable powder (1:5)

[0045] Formula composition: 5% Formula I compound, 25% abamectin, 4% tea saponin, 5% naphthalene sulfonate formaldehyde condensate, 3% BX splitting powder, and kaolin to make up the balance.

[0046] Preparation method: According to the formula ratio, the active ingredients, dispersant, wetting agent and filler are mixed and stirred evenly in a stirring tank. The mixture is then pulverized and mixed evenly multiple times by an air jet mill to prepare the wettable powder of the composition of the present invention.

[0047] Preparation Example 3: 24% Formula I compound·chlorfenapyr suspension (3:1)

[0048] Formula composition: 18% Formula I compound, 6% abamectin, 2% Gelbert alcohol polyoxyethylene ether, 2% naphthalene sulfonate formaldehyde condensate, 5% styrene phenol polyoxyethylene ether phosphate, 2% glycerol fatty acid ester polyoxyethylene ether, 0.2% xanthan gum, 1% magnesium aluminum silicate, 5% ethylene glycol, 0.2% potassium benzoate, 0.5% silicone oil, deionized water to make up the balance;

[0049] Preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in the reaction vessel in sequence, water is added and mixed evenly, and then subjected to high-speed shearing, wet sand milling and finally homogenization filtration to obtain the suspension product.

[0050] Preparation Example 4: 3% Compound I of Formula 1·chlorfenapyr aqueous emulsion (5:1)

[0051] Formula composition: 2.5% Formula I compound, 0.5% chlorfenapyr, 5% tristyrene-phenylphenol polyoxyethylene ether, 2% sodium dioctyl succinate sulfonate, 20% cyclohexanone, 5% thallium, 0.1% xanthan gum, 5% glycerol, 0.1% benzoic acid, deionized water to make up the balance.

[0052] Preparation method: According to the formula ratio, the active ingredient, solvent, emulsifier and cosolvent are added together to dissolve into a uniform oil phase; some water, antifreeze and other pesticide adjuvants are mixed together to form a uniform aqueous phase; while stirring at high speed in the reaction vessel, the oil phase is added to the aqueous phase, the shearing machine is turned on for high-speed shearing, and the remaining water is added. Shearing is carried out for about half an hour to form an oil-in-water emulsion.

[0053] Preparation Example 5: 2.7% Compound I of Formula 1·chlorfenapyr microemulsion (8:1)

[0054] Formula composition: 2.4% Formula I compound, 0.3% chlorfenapyr, 15% xylene, 18% cyclohexanone, 12% castor oil polyoxyethylene ether, 5% EO-PO block copolymer, 1% fatty alcohol polyoxyethylene ether sodium sulfate, 5% glycerol, 0.05% silicone defoamer, deionized water to make up the balance.

[0055] Preparation method: According to the formula ratio, the active ingredient solvent, emulsifier and other ingredients are mixed evenly to obtain the oil phase. The antifreeze and water are mixed evenly to obtain the aqueous phase. The oil phase is added to the aqueous phase under stirring and stirred evenly. Shearing is continued for 10 minutes. Then, silicone oil defoamer is added and stirred evenly to obtain small droplets with oil phase particles of 0.01 to 0.1 micrometers, which is the microemulsion of the present invention.

[0056] Example 1: Indoor bioassay of beet armyworm

[0057] Test basis: The test refers to NY / T 1154.6-2006 "Guidelines for Indoor Bioassay Tests of Pesticides - Insecticides Part 6: Insecticidal Activity Test - Immersion Method".

[0058] Experimental target: 3rd instar larvae of the beet armyworm.

[0059] Test reagents: chlorfenapyr technical grade, compound of formula I technical grade.

[0060] Experimental Method: The test drug was dissolved in a suitable solvent and then diluted with a 0.1% Tween 80 aqueous solution, setting five mass concentration gradients based on drug activity. Target insects were immersed in the solution for 8 seconds, then removed, excess solution was absorbed with filter paper, and the insects were transferred to normal rearing conditions. Each treatment was repeated four times, with 10 insects immersed in each replicate. A corresponding organic solvent treatment without the drug was included as a control. After 48 hours of treatment, the total number of insects and the number of dead insects were recorded. Insects were considered dead if they could not move coordinatedly or if their size differed significantly from the control when gently touched with a small brush or tweezers.

[0061] Calculation method:

[0062] Calculate the mortality rate for each treatment based on the survey data. Use the following formula:

[0063]

[0064] In the formula:

[0065] P – Mortality rate, expressed as a percentage (%);

[0066] K represents the number of dead insects, in heads;

[0067] N represents the total number of insects treated, in units of heads.

[0068]

[0069] In the formula:

[0070] P1 – Corrected mortality rate, in percentage (%);

[0071] P t —The mortality rate is expressed as a percentage (%).

[0072] P0 – Mortality rate in the blank control group, expressed as a percentage (%).

[0073] If the control mortality rate is <5%, no correction is needed; if the control mortality rate is between 5% and 20%, correction should be performed according to the corrected mortality rate formula; if the control mortality rate is >20%, the trial needs to be repeated.

[0074] The DPS statistical analysis system was used to analyze the data and obtain the virulence regression equation, correlation coefficient, and LC. 50 The value is used to evaluate the activity of the test reagent on the biological sample.

[0075] The co-toxicity coefficient (CTC value) of the mixture is calculated using the following formula:

[0076]

[0077] In the formula:

[0078] ATI – Actual Measured Toxicity Index of Mixtures;

[0079] S – LC50 of standard insecticides 50 The unit is milligrams per liter (mg / L);

[0080] M – LC of the mixture 50 The unit is milligrams per liter (mg / L).

[0081] TTI = TI A ×P A +TI B ×P B

[0082] In the formula:

[0083] TTI – Theoretical Toxicity Index of Mixtures;

[0084] TI A —A. Toxicity index of drug A;

[0085] P A —Percentage content of drug A in the mixture, expressed as percentage (%);

[0086] TI B —Toxicity index of drug B;

[0087] P B —Percentage content of agent B in the mixture, expressed as percentage (%).

[0088]

[0089] In the formula:

[0090] CTC – Cotoxicity Coefficient;

[0091] ATI – Actual Measured Toxicity Index of Mixtures;

[0092] TTI – Theoretical Toxicity Index of Mixtures.

[0093] The co-toxicity coefficient of the compound is ≥120, which shows a synergistic effect; CTC≤80 shows an antagonistic effect; and 80<CTC<120 shows an additive effect.

[0094] The results of the indoor tests are shown in the table below:

[0095] Table 1. Results of indoor bioactivity assays of compound I combined with chlorfenapyr for beet armyworm.

[0096]

[0097] The results of the indoor experiments (see Table 1) showed that the combination of compound I and chlorfenapyr in a suitable mass ratio range exhibited a significant synergistic effect against beet armyworm. Specifically, when the mass ratio of compound I to chlorfenapyr was 1:40–32:1, the co-toxicity coefficient against beet armyworm was greater than 120, indicating a synergistic effect; when the mass ratio was 1:30–16:1, the co-toxicity coefficient was greater than 130, showing a significant synergistic effect; when the mass ratio was 1:20–8:1, the co-toxicity coefficient was greater than 140, showing a remarkable synergistic effect; and when the mass ratio was 1:5, the co-toxicity coefficient against beet armyworm was the highest, at 182.410.

[0098] Example 2: Indoor bioassay of thrips

[0099] Experimental target: adult western flower thrips.

[0100] Test reagents: chlorfenapyr technical grade, compound of formula I technical grade.

[0101] Experimental Method: The leaf tube film method was used in this experiment. The original drug was first dissolved in a suitable solvent, then diluted with a 0.1% Tween 80 aqueous solution. Five mass concentration gradients were set according to the drug activity, with the corresponding organic solvent treatment without the drug serving as a blank control. The prepared drug was poured into 1.5 mL centrifuge tubes, left to stand for 4 hours, then the solution was discarded. The centrifuge tubes were placed on the experimental table to air dry naturally. A 2-3 mm hole was punched in the bottom of each centrifuge tube using a fine needle. Four replicates were set for each concentration, resulting in four centrifuge tubes. Fresh cabbage leaves were washed, dried, and punched into leaf discs. The leaves were immersed in the drug solution of each concentration for 10 seconds and then dried. The dried cabbage leaves and filter paper of the same size were placed at the bottom of the centrifuge tubes of the corresponding concentration. Ten test insects were then aspirated from each tube. The bottom cut was sealed with sealing film, the centrifuge tube was capped, and the tubes were placed in an incubator at (25±1)℃ with a photoperiod of L:D = 16h:8h. Check the number of dead test insects after 48 hours. The standard for death is that the test insects do not move at all when touched with a fine brush.

[0102] Calculation method:

[0103] Calculate the mortality rate for each treatment based on the survey data. Use the following formula:

[0104]

[0105] In the formula:

[0106] P – Mortality rate, expressed as a percentage (%);

[0107] K represents the number of dead insects, in heads;

[0108] N represents the total number of insects treated, in units of heads.

[0109]

[0110] In the formula:

[0111] P1 – Corrected mortality rate, in percentage (%);

[0112] P t —The mortality rate is expressed as a percentage (%).

[0113] P0 – Mortality rate in the blank control group, expressed as a percentage (%).

[0114] If the control mortality rate is <5%, no correction is needed; if the control mortality rate is between 5% and 20%, correction should be performed according to the corrected mortality rate formula; if the control mortality rate is >20%, the trial needs to be repeated.

[0115] The DPS statistical analysis system was used to analyze the data and obtain the virulence regression equation, correlation coefficient, and LC. 50 The value is used to evaluate the activity of the test reagent on the biological sample.

[0116] The co-toxicity coefficient (CTC value) of the mixture is calculated using the following formula:

[0117]

[0118] In the formula:

[0119] ATI – Actual Measured Toxicity Index of Mixtures;

[0120] S – LC50 of standard insecticides 50 The unit is milligrams per liter (mg / L);

[0121] M – LC of the mixture 50 The unit is milligrams per liter (mg / L).

[0122] TTI = TI A ×P A +TI B ×P B

[0123] In the formula:

[0124] TTI – Theoretical Toxicity Index of Mixtures;

[0125] TI A —A. Toxicity index of drug A;

[0126] P A —Percentage content of drug A in the mixture, expressed as percentage (%);

[0127] TI B —Toxicity index of drug B;

[0128] P B —Percentage content of agent B in the mixture, expressed as percentage (%).

[0129]

[0130] In the formula:

[0131] CTC – Cotoxicity Coefficient;

[0132] ATI – Actual Measured Toxicity Index of Mixtures;

[0133] TTI – Theoretical Toxicity Index of Mixtures.

[0134] The co-toxicity coefficient of the compound is ≥120, which shows a synergistic effect; CTC≤80 shows an antagonistic effect; and 80<CTC<120 shows an additive effect.

[0135] The results of the indoor tests are shown in the table below:

[0136] Table 2. Results of indoor bioactivity assays of chlorfenapyr and compound I in combination with western flower thrips.

[0137]

[0138] The results of the indoor tests (see Table 2) showed that the combination of compound I and chlorfenapyr in a suitable mass ratio range exhibited a significant synergistic effect on western flower thrips. Specifically, when the mass ratio of compound I to chlorfenapyr was 1:36–35:1, the co-toxicity coefficient against western flower thrips was greater than 120, indicating a synergistic effect; when the mass ratio was 1:18–20:1, the co-toxicity coefficient was greater than 130, showing a significant synergistic effect; when the mass ratio was 1:9–10:1, the co-toxicity coefficient was greater than 150, also showing a significant synergistic effect; and when the mass ratio was 3:1, the co-toxicity coefficient against western flower thrips was the highest, at 190.615.

[0139] Example 3: Field efficacy test for controlling beet armyworm and diamondback moth

[0140] Experimental location: This experiment was conducted at a vegetable base in Majinpu Township, Chenggong County, Kunming City, Yunnan Province. The soil at the experimental site was clay loess with medium to high soil fertility.

[0141] Experimental crops and targets: Cauliflower, beet armyworm and diamondback moth in a mixed outbreak.

[0142] Test plot: The area of ​​the test plot is 30m² 2 Each cell was arranged in a randomized block design, and each treatment was repeated 4 times.

[0143] Experimental Methods: The experiment was conducted once in mid-September 2023. A Gongnong-16 backpack manual sprayer was used to evenly spray the entire cauliflower plant. During the survey, five diagonal sampling points were used, with 10 plants at each point. The initial insect population was assessed before application, and the number of surviving insects was assessed on days 3 and 14 after application. The efficacy was calculated using the following formula.

[0144] Method for calculating the effectiveness of prevention:

[0145]

[0146] The experimental results are shown below:

[0147] Table 3 Results of field efficacy trials for controlling beet armyworm and diamondback moth

[0148]

[0149] As shown in Table 3, the control efficacy of each pesticide was 81.54%–93.22% 3 days after application, and 75.06%–98.38% 14 days after application.

[0150] Example 4: Field efficacy trial for controlling cucumber thrips

[0151] Experimental site: Cucumber greenhouse in Nanzhuang Village, Luocheng Street, Shouguang City, Shandong Province. The experimental site is a large arched greenhouse with a steel structure and moderate soil fertility.

[0152] Target species for prevention and control: Thrips.

[0153] Experimental crop: Cucumber (Jinyan No. 7).

[0154] Experimental setup: This experiment used a randomized block design, with each treatment replicated four times, and each plot area was 30m². 2 .

[0155] Experimental method: The pesticide was applied once using a uniform sprayer throughout the experiment. A Shandong Weishi WS-16 backpack manual sprayer was used. During spraying, both sides of the plant leaves were sprayed evenly until the pesticide was almost dripping.

[0156] Survey time and frequency: Survey the initial insect population before application of pesticide, and survey the number of live thrips 3 days and 7 days after application.

[0157] Survey Method: Ten plants were randomly sampled from the middle row of each plot. The number of live thrips on the upper two leaves of each plant was recorded. A total of 20 leaves were surveyed per plot, and the number of live thrips was recorded. Simultaneously, the safety of each treatment agent on flowers, tender shoots, and young leaves was observed, as well as any significant effects of the tested agents on other pests and beneficial organisms.

[0158] Methods for calculating drug efficacy:

[0159]

[0160] Results and Analysis:

[0161] Table 4 Results of field efficacy trials for controlling cucumber thrips

[0162]

[0163] Safety investigation: No phytotoxicity was observed in cucumber plants of any treatment group during the field trial, and no adverse effects were found on the surrounding environment or other beneficial organisms in the test area, indicating that each test agent is safe for cucumber growth at the supplied dosage.

[0164] The results (see Table 4) showed that the insecticidal composition of the present invention had a significant difference in efficacy compared to single agents. At 3 and 7 days post-application, the 24% Formula I compound·chlorfenapyr suspension (3:1), 30% Formula I compound·chlorfenapyr wettable powder (1:5), and 2.7% Formula I compound·chlorfenapyr microemulsion (8:1) exhibited significantly better rapid and sustained efficacy against thrips than the other agents. At 7 days post-application, the 24% Formula I compound·chlorfenapyr suspension (3:1) showed the highest efficacy, at 95.05%.

[0165] Although this application describes specific embodiments in detail with the aid of examples, the disclosure of this application can be modified and substituted in various ways. However, it should be understood that the disclosure of this application is not limited to the specific form disclosed. Rather, the disclosure of this application covers all modifications, equivalents, and substitutions within the scope of the disclosure of this application, the scope of which is defined by the appended claims and their legal equivalents.

Claims

1. An insecticidal composition containing chlorfenapyr, characterized in that, The active ingredient of the insecticidal composition comprises chlorfenapyr and a compound of formula I, the structure of which is as follows: The mass ratio of the compound of formula I to chlorfenapyr is 1:40 to 35:

1.

2. The insecticidal composition according to claim 1, characterized in that, The mass ratio of the compound of formula I to chlorfenapyr is 1:36 to 35:

1.

3. The insecticidal composition according to claim 2, characterized in that, The mass ratio of the compound of formula I to chlorfenapyr is 1:30 to 32:

1.

4. The insecticidal composition according to claim 3, characterized in that, The mass ratio of the compound of formula I to chlorfenapyr is 1:20 to 16:

1.

5. The insecticidal composition according to claim 1, characterized in that, In addition to the active ingredient, the insecticidal composition also includes pesticide-acceptable auxiliary ingredients, which are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, preservatives, stabilizers, synergists, or carriers.

6. The insecticidal composition according to claim 1, characterized in that, The total weight of the insecticidal composition is 100 wt%, and the total weight of the chlorfenapyr and the compound of formula I accounts for 0.1% to 80% of the insecticidal composition.

7. The insecticidal composition according to claim 1, characterized in that, The insecticidal composition is prepared into a pesticide formulation that is permitted in pesticides, wherein the formulation is a solid or liquid formulation.

8. The insecticidal composition according to claim 7, characterized in that, The solid formulation is a wettable powder or a water-dispersible granule, and the liquid formulation is a microemulsion, an emulsion, or a suspension.

9. The application of the insecticidal composition according to any one of claims 1-8 for the control of plant pests.

10. The application according to claim 9, characterized in that, The pests are Lepidoptera or Thysanoptera pests; the Lepidoptera pests are diamondback moth, beet armyworm, and cabbage caterpillar, and the Thysanoptera pests are thrips.

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