A plant growth regulator composition for reducing the number of thorns of cut rose and its preparation method and application

Abstract

The application provides a plant growth regulator composition for reducing the number of thorns of cut rose and a preparation method and application thereof, and belongs to the technical field of agricultural chemistry.The plant growth regulator composition for reducing the number of thorns of cut rose comprises the following raw materials: brassinazole, a polyurethane rheological agent and L-77 molecular surfactant.The preparation method is simple, fast in effect, low in cost, environmentally friendly and safe, can inhibit the formation of thorns at the stem part of cut rose in the growth process, and thus the number of thorns of cut rose is reduced, and the commercial value is greatly improved.

Description

A plant growth regulator composition for reducing the number of thorns in cut roses, its preparation method and application Technical Field

[0001] This invention relates to the field of agricultural chemistry, specifically to a plant growth regulator composition for reducing the number of thorns on cut roses, its preparation method, and its application. Background Technology

[0002] The rose (Rosa hybrida), belonging to the genus Rosa in the family Rosaceae, is a perennial evergreen or semi-evergreen low shrub that blooms year-round. It comes in a rich variety of colors and types and is used for ornamental and medicinal purposes, as well as being widely used as a cut flower. As one of the world's four major cut flowers, the rose has high economic value and is one of the most consumed flowers in the cut flower market.

[0003] Cut roses are a high-value economic crop. Their commercial value and market competitiveness are influenced not only by flowering quality but also by post-harvest processing costs and consumer experience. In Yunnan Province, a major rose-producing area in my country, thorns are one of the main problems encountered during harvesting and post-harvest processing. Enterprises and flower growers need to invest significant manpower in thorn removal to ensure the cut flowers meet market standards. However, relying on manual thorn removal is not only inefficient and costly but also easily damages the flower stem's epidermis during the process, affecting the quality and vase life of the cut flowers. Surveys have found that after traditional manual thorn removal, the rate of grade reduction due to epidermal scratches and infections is as high as 15-20%, severely restricting the improvement of the rate of high-quality commercial flowers. Furthermore, industry research has revealed that epidermal damage to the flower stem is a major cause of bacterial vascular blockage during post-harvest distribution, directly accelerating the wilting and decay of cut flowers and reducing end-consumer satisfaction. For example, if the mainstream cut flower variety 'Red Boat' suffers multiple damages during thorn removal, its vase life may be shortened by 3-5 days, and its appearance quality and value will significantly decrease. Therefore, the number of thorns on rose branches directly determines post-harvest processing efficiency, loss rate, and the final consumer experience, thus affecting the economic benefits for businesses and flower growers. Therefore, exploring methods to fundamentally reduce the number of thorns on cut rose branches is of paramount industrial significance. Summary of the Invention

[0004] The purpose of this invention is to provide a plant growth regulator composition for reducing the number of thorns on cut roses, its preparation method, and its application. The preparation method is simple, fast-acting, low-cost, and environmentally friendly and safe. It can inhibit the formation of thorns on the stems of cut roses during their growth, thereby reducing the number of thorns and significantly increasing their commercial value.

[0005] The technical solution of this invention is implemented as follows:

[0006] This invention provides a plant growth regulator composition for reducing the number of thorns on cut roses, comprising the following raw materials: brassinoazole, polyurethane rheology modifier, and surfactant L-77. Using plant hormones as the main raw material, it is environmentally friendly, safe, pollution-free, and low-cost, ensuring normal hormone signal transduction and developmental regulation, thereby regulating the development process of cut rose thorns and enhancing the value of cut flower products.

[0007] As a further improvement of the present invention, the raw materials include the following parts by weight: 6-8 parts of brassinoazole, 6-8 parts of polyurethane rheology modifier, and 6-8 parts of surfactant L-77.

[0008] As a further improvement of the present invention, the polyurethane rheology modifier includes an associative polyurethane polymer, a carrier solvent, and functional additives.

[0009] This invention further protects a method for preparing the above-mentioned plant growth regulator composition for reducing the number of thorns in cut roses, comprising the following steps:

[0010] Brassinolide, polyurethane rheology modifier, and surfactant L-77 are added to water and mixed thoroughly. A measured amount of water is then added, and the mixture is thoroughly shaken and mixed. The pH of the mixture is adjusted to obtain a plant growth regulator composition that reduces the number of thorns on cut roses. While ensuring flower quality (important traits such as flower color and shape), the plant growth regulator composition developed in this invention has a simple preparation process, rapid effect, low cost, and is environmentally friendly and safe. It also has a significant effect, effectively reducing the number of thorns, thereby improving the quality of cut roses, increasing harvesting efficiency, and enhancing industry benefits.

[0011] As a further improvement of the present invention, the pH of the mixture is adjusted to 5.8-6.0.

[0012] Furthermore, the plant growth regulator composition that reduces the number of thorns on cut roses is used to achieve precise control from the red-striped stage to the green stage of cut roses.

[0013] The present invention further protects the application of the above-mentioned plant growth regulator composition for reducing the number of thorns in cut roses in reducing the number of thorns in cut roses, and uses the plant growth regulator composition for reducing the number of thorns in cut roses in the production of cut roses.

[0014] Furthermore, the plant growth regulator composition that reduces the number of thorns on cut roses is used to achieve precise control from the red-striped stage to the green stage of cut roses.

[0015] As a further improvement of the present invention, the method of using the plant growth regulator composition for reducing the number of thorns in cut roses is to spray the base of cut rose plants in the red stripe stage with the plant growth regulator composition for reducing the number of thorns in cut roses. Using the plant growth regulator composition developed in this invention can effectively reduce the number of thorns in cut roses and increase the added value of cut roses.

[0016] Preferably, the spraying time is 16:00-18:00 on a sunny afternoon. The plants should be kept dry during spraying, and spraying should be avoided during the high temperature at noon.

[0017] The present invention has the following beneficial effects:

[0018] Brassinazole is a selective triazole brassinosteroid (BR) biosynthesis inhibitor that can regulate plant architecture, improve photosynthetic efficiency, reduce the number of thorns, enhance resistance to fungal diseases, and increase tolerance to abiotic stresses such as drought, low temperature, and salinity. Polyurethane rheology modifiers can efficiently construct a three-dimensional network structure for the regulator, thereby significantly improving its viscosity and yield value, preventing sagging and splashing during spraying, and ensuring uniform coating thickness. Surfactant L-77 can significantly reduce the surface tension of the regulator, optimize adhesion performance, promote rapid penetration of the regulator through stomata and epidermis, enhance its wetting and spreading ability, improve spraying efficiency and uniformity, thereby ensuring full coverage of the stem and accelerating the absorption and translocation of active ingredients.

[0019] The plant growth regulator composition of the present invention, which reduces the number of thorns in cut roses, uses plant growth regulators as the main raw material. The preparation method is simple, fast-acting, low-cost, and environmentally friendly and safe. It effectively reduces the number of thorns in cut roses, thus addressing the industry problem and aiming to improve the quality of cut roses, increase harvesting efficiency, and enhance industry benefits. Attached Figure Description

[0020] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0021] Figure 1 shows the effect of different treatments on the number of burs in this invention;

[0022] Figure 2 shows a comparison of the number of burs under different treatments in this invention;

[0023] Figure 3 shows the effect of different treatments on the vase life of flowers after harvesting in this invention.

[0024] Figure 4 shows a comparison of the duration of flower vase life after harvesting under different treatments in this invention. Detailed Implementation

[0025] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0026] In a specific embodiment of the present invention, the brassinoazole used was purchased from MCE (MedChemExpress) in the market, catalog number: HY-121161A, CAS: 280129-83-1; the polyurethane rheology modifier was purchased from Jiangsu Jintu Chemical Co., Ltd. (Jintu Additives), with an effective content of 25% and model WZ341; and the surfactant L-77 was purchased from Beijing Solarbio Technology Co., Ltd., Lot. No.: 510F021, Cat. No.: S9430.

[0027] Example 1: Preparation of plant growth regulator composition

[0028] (1) Add 40 μL of anhydrous ethanol to 1 mg of brassinoazole, and after it is fully dissolved, add water to make up to 1 mL to prepare a 1 mg / mL brassinoazole stock solution; the polyurethane rheology modifier stock solution uses Jintu auxiliary polyurethane rheology modifier agent, and its polyurethane effective content is 25%; the surfactant L-77 stock solution uses Solarbio surfactant L-77 agent, and its surfactant L-77 effective content is 0.1%;

[0029] (2) Add 164 μL of brassinoazole stock solution, 200 μL of polyurethane rheology modifier stock solution, and 5 μL of surfactant L-77 stock solution to 25 mL of water, add water to make up to 50 mL, shake and mix thoroughly, and adjust the pH of the mixture to 6.0 to obtain a plant growth regulator composition that reduces the number of thorns on cut roses.

[0030] Example 2: Preparation of Plant Growth Regulator Composition

[0031] (1) Add 40 μL of anhydrous ethanol to 1 mg of brassinoazole, and after it is fully dissolved, add water to make up to 1 mL to prepare a 1 mg / mL brassinoazole stock solution; the polyurethane rheology modifier stock solution uses Jintu auxiliary polyurethane rheology modifier agent, and its polyurethane effective content is 25%; the surfactant L-77 stock solution uses Solarbio surfactant L-77 agent, and its surfactant L-77 effective content is 0.1%;

[0032] (2) Add 820 μL of brassinoazole stock solution, 200 μL of polyurethane rheology modifier stock solution, and 5 μL of surfactant L-77 stock solution to 25 mL of water, add water to make up to 50 mL, shake and mix thoroughly, and adjust the pH of the mixture to 6.0 to obtain a plant growth regulator composition that reduces the number of thorns on cut roses.

[0033] Example 3: Use of plant growth regulator composition

[0034] On sunny afternoons at 5:00 PM, when the plants are dry, spray the base of the stems of roses in the red-striped stage (normal growth to 15-25cm after axillary bud sprouting) every three days, for a total of three sprays. At other times, the roses are managed using conventional production techniques in an intelligent multi-span greenhouse. The daytime temperature in the greenhouse is 25-30℃; the nighttime temperature is 15-20℃. The roses are planted in soilless cultivation troughs with a 3:1 mixture of coconut coir and perlite as the substrate. Water and fertilizer management is implemented (maintaining soil EC=1.5, substrate pH=5.8-6.0, watering 8-12 times, and maintaining substrate moisture at 60%). The frequency of watering and fertilization is adjusted according to weather conditions. Special attention is also paid to pests and diseases (such as spider mites). For the prevention and control of pests such as spider mites, aphids, thrips, powdery mildew, and gray mold, apply pesticides every 7 days (for spider mites: abamectin, 1.8% active ingredient, spray concentration 800 times; for aphids: acetamiprid, 70% active ingredient, spray concentration 600 times; for thrips: bromuconazole, 5% active ingredient, spray concentration 1000 times; for powdery mildew: ethirimol, 25% active ingredient, spray concentration 800 times; for gray mold: pyrimethanil, 70% active ingredient, spray concentration 1500 times) for prevention and control.

[0035] test:

[0036] Using the cut rose 'Red Boat' from the Baofeng Base of the Yunnan Academy of Agricultural Sciences' Flower Research Institute as experimental material, 45 plants of similar size and quality at the red-striped stage (normally grown to 15-25 cm after axillary bud sprouting) were selected and divided into 3 groups of 15 plants each, with clear labels. The grouping is as follows:

[0037] Treatment Group 1: The plant growth regulator composition prepared in Example 1 was selected; the spraying method was selected from the method in Example 7.

[0038] Treatment group 2: The plant growth regulator composition prepared in Example 2 was selected; the spraying method was selected from the method in Example 7.

[0039] Control group: Water was selected as the control; the spraying method was the method of Example 7.

[0040] The first spraying for each group was on the afternoon of August 31, 2025, under sunny weather conditions. The plants were finally pruned on October 6, 2025, for a total of three sprayings. On October 6, photos, statistical analysis, and vase-standing observations were conducted on the control group and treatment groups 1-2.

[0041] As shown in Figures 1-4: The plants in the control group and the treatment group were treated on August 31, 2025. The flowers reached the harvest period on October 6, 2025. The plants were harvested uniformly and photographed, counted, and observed in vases at the same time.

[0042] Compared with the control, treatment groups 1 and 2 significantly reduced the number of thorns. Both 10 μM brassinolide and 50 μM brassinolide significantly inhibited thorn formation, reducing the number of thorns by 46.15% and 63.80% respectively compared to the control. Simultaneously, compared with the control, treatment groups 1 and 2 significantly prolonged the vase life of cut flowers. Both 10 μM and 50 μM brassinolide significantly prolonged vase life by reducing the number of thorns, increasing it by 32.26% and 35.48% respectively compared to the control. Therefore, the plant growth regulator composition prepared in this invention, which reduces the number of thorns in cut roses, addresses the problems of low post-harvest efficiency and short vase life caused by a large number of thorns in cut rose production. It can reduce the number of thorns in roses, thereby improving the quality and harvesting efficiency of cut roses, increasing the value of fresh cut flowers, and providing practical support for the steady improvement of quality and efficiency in my country's cut rose industry.

[0043] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A plant growth regulator composition for reducing the number of thorns on cut roses, characterized in that, The raw materials include the following parts by weight: 6-8 parts brassinoazole, 6-8 parts polyurethane rheology modifier, and 6-8 parts surfactant L-77.

2. The plant growth regulator composition for reducing the number of thorns in cut roses according to claim 1, characterized in that, The polyurethane rheology modifier includes an associative polyurethane polymer, a carrier solvent, and functional additives.

3. A method for preparing a plant growth regulator composition for reducing the number of thorns in cut roses as described in claim 1 or 2, characterized in that, The process includes the following steps: adding brassinoazole, polyurethane rheology modifier, and surfactant L-77 to water, mixing them evenly, adding a measured amount of water, shaking and mixing thoroughly, and adjusting the pH of the mixture to obtain a plant growth regulator composition that reduces the number of thorns on cut roses.

4. The preparation method according to claim 3, characterized in that, Adjust the pH of the mixture to 5.8-6.

0.

5. The use of a plant growth regulator composition for reducing the number of thorns in cut roses as described in claim 1 or 2 in reducing the number of thorns in cut roses.

6. The application according to claim 5, characterized in that, The method of using the plant growth regulator composition for reducing the number of thorns on cut roses is to spray the base of the cut rose plant in the red stripe stage with the plant growth regulator composition for reducing the number of thorns on cut roses.

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