Formed component detection dry reagent, redissolving reagent and microscopic examination sample preparation method

Abstract

In the preparation method of the visible component detection dry reagent, the redissolving reagent and the microscopic examination sample, the visible component detection dry reagent is formed by drying a liquid dyeing reagent; the liquid staining reagent comprises a staining agent and an acid-base regulator; the pH regulator is used for regulating the pH value of the liquid dyeing reagent; the liquid dyeing reagent is filtered before being dried. Before drying, dividing the liquid dyeing reagent into small parts, and drying separately; the liquid dyeing reagent is divided into single parts with set volume, and then is dried to remove moisture. The single part of the set capacity is 300 ul to 3000 ul per piece. The visible component is used for detecting the dry reagent, so that the reagent is more convenient to store and transport, and the quality guarantee period is longer.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of formed component detection, and particularly relates to a formed component detection dry reagent, a reconstitution reagent and a preparation method of a formed component detection microscopic sample. BACKGROUND

[0002] The preparation of a formed component detection microscopic sample involves various types of detection objects, and different types of detection objects usually require different microscopic sample processing methods to make the microscopic sample suitable for microscopic magnification imaging under bright field and enable AI recognition based on the image of the microscopic magnification imaging.

[0003] The preparation of a formed component detection microscopic sample in the prior art is usually based on a liquid reagent, and therefore the packaging and transportation of the reagent are burdensome, and the shelf life of the liquid reagent is short. How to design a reagent that is more suitable for packaging and transportation and has a longer shelf life is also a technical problem to be solved. Different components have different properties, and if mixed directly in a dry powder state, the effect of the preparation of a detection microscopic sample at the client end is not good, the time required is long, and the consistency of the effect of the preparation of a microscopic sample is difficult to guarantee. In particular, the consistency of the staining effect is difficult to guarantee.

[0004] How to prepare a microscopic sample, in which the reagent plays a key role. Different detection objects in the prior art usually require the design of reagents with different component formulations. How to design a simple reagent that can be compatible with multiple detection objects is a technical problem to be solved.

[0005] The applicant has proposed a series of Chinese patents, such as

[0006] 1. CN2020112669290, "Cell analysis method and system and quantitative method and system";

[0007] 2. CN2020112669182, "Cell suspension sample imaging method and system and kit";

[0008] 3. CN2022104799126, "Microscopic image acquisition device rapid focusing method and microscopic image acquisition method";

[0009] 4. CN2023116834572, "Blood leukocyte recognition AI training method and computing processing and storage device".

[0010] With a brand-new technical solution, based on image processing technology of artificial intelligence algorithm, through analysis and learning of images, recognition and understanding of image content are realized; and through artificial intelligence algorithm, the content of various formed components in blood is measured, including analysis, recognition and counting of red blood cells and white blood cells at different differentiation stages in blood.

[0011] In the recognition based on bright field microscopic magnification images, the highlighting of different types of cell features is needed by means of staining to facilitate machine learning and recognition. Since there are many types of target cells that need to be stained, and the characteristics of different cells are different, if the staining effect is very different, it will lead to an increase in learning workload, or abnormal or incorrect recognition.

[0012] In the prior art, in the above application scenarios, when preparing the microscopic sample, staining is usually based on two-step reagents, the first step is to perform staining, and then to perform staining solidification. A sufficient time interval is needed between the two steps to maintain good staining effect. Although the two-step method has guaranteed staining effect, the sample preparation efficiency is not high. How to improve the staining efficiency under the premise of guaranteeing the staining effect is a technical problem to be solved.

[0013] Glossary:

[0014] Microscopic sample: a sample that can be injected into a detection chip to obtain a microscopic magnification image.

[0015] CAS number (CAS Registry Number or CAS Number, CAS Rn, CAS #) is the unique digital identification number of a certain substance (compound, polymer material, biological sequence, mixture or alloy). The CAS numbers of some substances in this application are as follows: Figure 15 . SUMMARY

[0016] In this application, the inventors propose a formed component detection dry reagent, which makes the storage and transportation of the reagent more convenient, has a longer shelf life, high staining efficiency, can realize efficient and stable staining, supports formed component analysis, and adding glycerol to the formed component analysis dry reagent can enhance its antioxidant property and increase the shelf life of the reagent.

[0017] The technical solution of the present application is a formed component detection dry reagent, and the formed component detection dry reagent is formed by drying a liquid staining reagent; the liquid staining reagent includes a staining agent and an acid-base regulator; the acid-base regulator is used to adjust the pH value of the liquid staining reagent; the liquid staining reagent is filtered before drying.

[0018] The formed component detection dry reagent includes any of the following technical features: TA1: before drying, the liquid staining reagent is divided into small portions and dried separately; TA2: the liquid staining reagent is divided into single portions with a set capacity and then dried to remove water.

[0019] The single portion with a set capacity is 300ul-3000ul per bottle.

[0020] The 0.8 μm filter head is used to filter the liquid-state dyeing reagent once; the 0.8 μm filter head is used to filter the liquid-state dyeing reagent once, and then the 0.22 μm filter head is used to filter the liquid-state dyeing reagent twice; the dyeing reagent is any one or more of methylene blue, new methylene blue, carbolic tar blue, toluidine blue, neutral red, crystal violet, and methyl green; the reference particles are added to the liquid-state dyeing reagent after the liquid-state dyeing reagent is filtered; the reference particles are organic silicon particles, and the concentration of the organic silicon particles in the liquid-state dyeing reagent ranges from 1.3 × 10 -2 g / L to 5.0 × 10 0 g / L; the acid-base regulator is used to adjust the pH value of the smear sample for the detection of the formed elements to a weak alkaline environment with a pH value of 7-8; the acid-base regulator comprises any one or more of Hepes-NaOH, Tris-HCl, MES-NaOH, phosphate buffer, phosphate buffer, and imidazole buffer; the osmotic pressure regulating salt is used to adjust the smear sample for the detection of the formed elements to a low osmotic pressure environment; the osmotic pressure regulating salt comprises sodium chloride; the dyeing promoter comprises an EDTA salt; and the preservative comprises preservative 950.

[0021] The smear sample for the detection of the formed elements comprises any one of the following features: the dyeing reagent is new methylene blue, and the concentration of the new methylene blue in the smear sample for the detection of the formed elements ranges from 4.0 × 10 -3 g / L to 5.0 × 10 -1 g / L; the acid-base regulator comprises disodium hydrogen phosphate and potassium dihydrogen phosphate, and the concentration of the disodium hydrogen phosphate and the potassium dihydrogen phosphate in the smear sample for the detection of the formed elements ranges from 2.0 × 10 -2 mol / L to 1.0 × 10 -1 mol / L; the osmotic pressure regulating salt comprises sodium chloride, and the concentration of the sodium chloride in the smear sample for the detection of the formed elements ranges from 2.2 × 10 -2 mol / L to 3.5 × 10 -2 mol / L; the dyeing promoter comprises an EDTA salt, and the concentration of the EDTA salt in the smear sample for the detection of the formed elements ranges from 2.0 × 10 -4 mol / L to 2.0 × 10 - 3mol / L; TXE5: further comprising a preservative; in the microscopic sample for detecting formed elements, the volume percentage of the preservative is 0.1%; TXE6: further comprising glycerol; the concentration range of the glycerol in the microscopic sample for detecting formed elements is 1.0 x 10 -1 mol / L ~ 8.1 x 10 - 1 mol / L; TXE7: the staining agent is crystal violet, and the concentration range of the crystal violet in the microscopic sample for detecting formed elements is 6.2 x 10 -2 g / L ~ 1.7 x 10 -0 g / L; TXE8: the staining agent is methylene blue, and the concentration range of the methylene blue in the microscopic sample for detecting formed elements is 7.4 x 10 -2 g / L ~ 9.8 x 10 -1 g / L.

[0022] The above dry reagent for detecting formed elements is used for urine formed element detection, and comprises any one of the following characteristics: TNE1: the staining agent is new methylene blue, and the concentration range of the new methylene blue in the microscopic sample for detecting formed elements is 7.2 x 10 -4 mol / L ~ 2.9 x 10 -2 mol / L; TNE2: further comprising an acid-base regulator, and the acid-base regulator comprises disodium hydrogen phosphate and potassium dihydrogen phosphate; the concentration range of the disodium hydrogen phosphate in the microscopic sample for detecting formed elements is 8.4 x 10 -2 mol / L ~ 1.6 x 10 - 1 mol / L; the concentration range of the potassium dihydrogen phosphate in the microscopic sample for detecting formed elements is 2.0 x 10 -2 mol / L ~ 3.7 x 10 - 2 mol / L; TNE3: further comprising reference particles, and the reference particles are organic silicon particles, and the concentration range of the organic silicon particles in the microscopic sample for detecting formed elements is 1.3 x 10 -2 g / L ~ 5.0 x 10 0 g / L; TNE4: further comprising a preservative; in the microscopic sample for detecting formed elements, the volume percentage of the preservative is 0.1%; TNE5: further comprising glycerol; the concentration range of the glycerol in the microscopic sample for detecting formed elements is 1.0 x 10 -1 mol / L ~ 8.1 x 10 -1 mol / L.

[0023] The above dry reagent for detecting formed elements is used for fecal formed element detection, and comprises any one of the following characteristics: TFE1: the staining agent is new methylene blue, and the concentration range of the new methylene blue in the microscopic sample for detecting formed elements is 4.3 x 10 -4 mol / L ~ 1.7 x 10-2 mol / L; TFE2: further comprising acid-base regulator, the acid-base regulator comprises disodium hydrogen phosphate and potassium dihydrogen phosphate; the concentration range of the disodium hydrogen phosphate in the formed element detection microscopic sample is 6.8*10 -2 mol / L~1.3*10 - 1 mol / L; the concentration range of the potassium dihydrogen phosphate in the formed element detection microscopic sample is 2.6*10 -2 mol / L~4.7*10 - 2 mol / L; TFE3: further comprising barium sulfate, the concentration range of the barium sulfate in the formed element detection microscopic sample is 2.5*10 -1 mol / L~3.9*10 0 mol / L; TFE4: further comprising preservative; the volume ratio of the preservative in the formed element detection microscopic sample is 0.1%; TFE5: further comprising glycerol; the concentration range of the glycerol in the formed element detection microscopic sample is 3.4*10 - 1 mol / L~2.7*10 -0 mol / L.

[0024] A formed element detection microscopic sample preparation method based on the formed element detection dry reagent.

[0025] A reconstitution reagent for reconstituting the formed element detection dry reagent, comprising fixative; the concentration range of the fixative in the formed element detection microscopic sample is 1.4*10 -3 mol / L~5.3*10 -2 mol / L.

[0026] A formed element detection microscopic sample preparation method for blood formed element detection microscopic sample preparation based on the formed element detection dry reagent and the reconstitution reagent; comprising the following steps: A10: mixing the reconstitution reagent and the formed element detection dry reagent to form a staining solution; A20: adding the blood sample into the staining solution to prepare the formed element detection microscopic sample.

[0027] A reconstitution reagent for reconstituting the formed element detection dry reagent, comprising fixative; the concentration range of the fixative in the formed element detection microscopic sample is 3.8*10 -3 mol / L~5.8*10 -1 mol / L; comprising glycerol; the concentration range of the glycerol in the formed element detection microscopic sample is 3.4*10 -1 mol / L~2.7*10 -0 mol / L.

[0028] A formed element detection microscopy sample preparation method for fecal formed element detection microscopy sample preparation, based on the formed element detection dry reagent of claim 8; based on the reconstitution reagent; comprising the following steps: B10: mixing the reconstitution reagent and the formed element detection dry reagent to form a staining solution; B20: adding the fecal sample to the staining solution to prepare a formed element detection microscopy sample.

[0029] A formed element detection microscopy sample preparation method for urine formed element detection microscopy sample preparation, based on the formed element detection dry reagent; comprising the following steps: C20: mixing the urine sample and the formed element detection dry reagent to prepare a formed element detection microscopy sample.

[0030] One of the technical effects of the above technical solution is that the formed element detection freeze-dried reagent is used for microscopic examination of formed elements, and filtration can eliminate particulate matter in the staining base solution or staining solution, eliminating the influence of particulate matter on microscopy and image recognition, and making the formed element detection microscopy more accurate.

[0031] One of the technical effects of the above technical solution is that the formed element detection dry reagent makes the storage and transportation of the reagent more convenient and has a longer shelf life.

[0032] One of the technical effects of the above technical solution is that the acid-base regulator can provide a suitable pH, providing a suitable environment for rapid one-step staining.

[0033] One of the technical effects of the above technical solution is that the aforementioned liquid staining reagent or staining solution is divided into small portions and dried separately, which can accurately control the staining agent content of each portion.

[0034] One of the technical effects of the above technical solution is that the liquid staining reagent or staining solution is divided into single portions of a set volume, and then dried to remove moisture, facilitating the preparation of single reagents.

[0035] One of the technical effects of the above technical solution is that the single portion of a set volume is 300ul-3000ul per bottle, which is convenient for packaging.

[0036] One of the technical effects of the above technical solution is that the liquid staining reagent can remove impurities of different sizes by using different size filters, reducing the impact of impurities on quality.

[0037] One of the technical effects of the above technical solution is that it can be used for formed element detection in blood, urine, body cavity fluid or feces, and has a wide range of applications.

[0038] One of the technical effects of the above technical solution is that the staining agent has a variety of types and is more flexible in staining, suitable for identification of different types of cells.

[0039] One of the technical effects of the above technical solution is that the reference particles provide a contrast reference for formed element analysis.

[0040] One of the technical effects of the above-mentioned technical solution is that the osmotic pressure regulating salt can provide a suitable osmotic pressure, providing a suitable atmosphere for rapid single-step staining.

[0041] One of the technical effects of the above-mentioned technical solution is that the dyeing accelerator promotes the affinity between the dye and intracellular nucleic acid substances during the staining process.

[0042] One of the technical advantages of the above-mentioned solution is that the reconstitution reagent includes a fixative; the aldehydes or phenols in the fixative react with proteins on the cell membrane surface, causing protein denaturation and inactivation, making it easier for the dye to penetrate the cell membrane and achieving single-step rapid staining. Setting the concentration of the fixative within an appropriate range allows the staining effect of the reconstituted formed elements to be maintained for a longer period.

[0043] One of the technical effects of the above-mentioned technical solution is that preservative 950 can increase the shelf life of the reagent.

[0044] One of the technical advantages of the above-mentioned technical solution is that the dry reagents for the detection of formed elements are convenient to carry and transport.

[0045] One of the technical effects of the above-mentioned technical solution is that the reconstitution reagent enables the dry reagent for the detection of formed elements to be quickly reconstituted and used for the preparation of microscopic samples for the detection of formed elements.

[0046] One of the technical advantages of the above-mentioned technical solution is that, based on the dry reagent and reconstitution reagent for the detection of formed elements in the preparation method of microscopic samples, the preparation process of microscopic samples for the detection of formed elements can be carried out quickly, and the preparation process is simple, efficient and has good staining effect.

[0047] One of the technical effects of the above-mentioned technical solution is that the method for preparing blood formed element detection microscopic samples, by setting appropriate reconstitution reagents, ensures the quality of blood formed element detection microscopic sample preparation.

[0048] One of the technical effects of the above-mentioned technical solution is that the method for preparing fecal microscopic samples for the detection of formed elements involves setting up appropriate reconstitution reagents to ensure the quality of the prepared fecal microscopic samples.

[0049] One of the technical effects of the above-mentioned technical solution is that the reconstitution reagent includes glycerol, which has better antioxidant properties and the glycerol component can protect the morphology of protozoa; in the detection of fecal parasites, it can maintain better morphological characteristics of protozoa, which is conducive to the accuracy of formed element analysis.

[0050] One of the technical advantages of the above-mentioned solution is that the method for preparing urine sample for microscopic examination of formed elements does not require the use of corresponding reconstitution reagents, which ensures the quality of urine sample preparation for microscopic examination of formed elements while being simpler and more efficient. Attached Figure Description

[0051] Figures 1 to 5 is a schematic diagram of a particulate detection dry reagent and a method of preparing the same Figures 1 to 5 ;

[0052] Figures 6 to 14 is a schematic table of components of various embodiments of a particulate detection dry reagent

[0053] Figure 15 is the CAS number of some substances DETAILED DESCRIPTION

[0054] The present application will be further described by examples in conjunction with the accompanying drawings. It should be noted that the following examples are illustrative of specific embodiments of the application and are not meant to limit the present application in any way. The following examples are merely illustrative of the principles of the application. Further, the description of the examples is merely meant to illustrate the general principles of the application. The use of "first", "second", "A" and "B" does not indicate any order or sequence unless otherwise specifically indicated.

[0055] As Figure 1 , an embodiment of a particulate detection dry reagent, the particulate detection dry reagent is formed by drying a liquid staining reagent; the liquid staining reagent includes a staining agent, an acid-base adjusting agent; the acid-base adjusting agent is used to adjust the pH value of the liquid staining reagent; the liquid staining reagent is filtered before drying.

[0056] As Figure 2 , the liquid staining reagent is divided into small portions before drying and dried separately. As Figure 3 , the liquid staining reagent is divided into single portions of a set volume and then dried to remove water. The single portion of the set volume is 300ul-3000ul per portion.

[0057] As Figure 4 , the liquid staining reagent is filtered once with a 0.8um filter head; after the first filtration, the liquid staining reagent is filtered again with a 0.22um filter head.

[0058] As Figure 5 , an embodiment of a particulate detection dry reagent, further includes reference particles; the reference particles are prepared into a particle solution and added to the liquid staining reagent after the liquid staining reagent is filtered; then dried.

[0059] In some embodiments of the particulate detection dry reagent, the staining agent is any one or more of methylene blue, new methylene blue, aniline blue, toluidine blue, neutral red, crystal violet, and methyl green.

[0060] In some embodiments of the particulate detection dry reagent, further includes reference particles, the reference particles are silicone particles, the concentration of the silicone particles in the liquid staining reagent is in the range of 1.3x10-2 g / L~5.0×10 0 g / L.

[0061] In some embodiments of the dry reagents for formed element detection, an acid-base adjuster is used to adjust the pH of the microscopic sample for formed element detection to a weakly alkaline environment of 7-8.

[0062] In some examples of dry reagents for the detection of formed elements, an acid-base adjuster is included, which includes any one or more of Hepes-NaOH, Tris-HCl, MES-NaOH, phosphate buffer, phosphate buffer, and imidazole buffer.

[0063] In some embodiments of the dry reagent for the detection of formed elements, an osmotic pressure regulating salt is also included, which is used to adjust the microscopic sample for the detection of formed elements to a low osmotic pressure environment;

[0064] In some embodiments of the dry reagent for the detection of formed elements, an osmotic pressure regulating salt is also included, which includes sodium chloride;

[0065] In some embodiments of the dry reagent for the detection of formed elements, a staining accelerator is also included, which includes EDTA salts;

[0066] In some embodiments of the dry reagent for the detection of formed elements, a preservative is also included, including preservative 950.

[0067] like Figure 6 and Figure 7 In some examples of dry reagents for the detection of formed elements, used for the detection of formed elements in blood, the staining agent is neomethylene blue, and the concentration range of the aforementioned neomethylene blue in the microscopic samples for the detection of formed elements is 4.0 × 10⁻⁶. -3 g / L~5.0×10 -1 g / L; acid-base adjusters include disodium hydrogen phosphate and potassium dihydrogen phosphate; the concentration range of the above disodium hydrogen phosphate and potassium dihydrogen phosphate in microscopic samples for formed element detection is 2.0 × 10 g / L. -2 mol / L~1.0×10 -1 mol / L; also includes osmotic pressure regulating salts, including sodium chloride; sodium chloride, in the microscopic samples for formed element detection, has a concentration range of 2.2 × 10⁻⁶ mol / L. -2 mol / L~3.5×10 -2 mol / L; also includes staining accelerators, including EDTA salts; the concentration range of EDTA salts in microscopic samples for formed element detection is 2.0 × 10⁻⁶. -4 mol / L~2.0×10 -3 mol / L; also includes preservatives; in microscopic samples with formed elements detected, the volume percentage of preservatives is 0.1%. For example... Figure 7In some embodiments of the dry reagent for detecting the formed elements, the glycerol is present in the dry reagent for detecting the formed elements in a concentration range of 1.0 x 10 -1 mol / L to 8.1 x 10 -1 mol / L.

[0068] In some embodiments of the dry reagent for detecting the formed elements, the crystal violet is present in the dry reagent for detecting the formed elements in a concentration range of 6.2 x 10 -2 g / L to 1.7 x 10 -0 g / L.

[0069] In some embodiments of the dry reagent for detecting the formed elements, the methylene blue is present in the dry reagent for detecting the formed elements in a concentration range of 7.4 x 10 -2 g / L to 9.8 x 10 -1 g / L.

[0070] As Figure 8 In some embodiments of the dry reagent for detecting the formed elements, the fixative is present in the dry reagent for detecting the formed elements in a concentration range of 1.4 x 10 -3 mol / L to 5.3 x 10 -2 mol / L.

[0071] A method for preparing a microscope sample for detecting the formed elements, for preparing a microscope sample for detecting the formed elements in blood, based on the dry reagent for detecting the formed elements described above, based on the reconstitution reagent described above, comprising the following steps: A10: mixing the reconstitution reagent and the dry reagent for detecting the formed elements to form a staining solution; A20: adding the blood sample to the staining solution to prepare the microscope sample for detecting the formed elements.

[0072] As Figure 13 and Figure 14 In some embodiments of the dry reagent for detecting the formed elements, for detecting the formed elements in urine, the new methylene blue is present in the dry reagent for detecting the formed elements in a concentration range of 7.2 x 10 -4 mol / L to 2.9 x 10 -2 mol / L; the acid-base regulator is present in the dry reagent for detecting the formed elements in a concentration range of 8.4 x 10 -2 mol / L to 1.6 x 10 -1 mol / L; and the potassium dihydrogen phosphate is present in the dry reagent for detecting the formed elements in a concentration range of 2.0 x 10 -2 mol / L to 3.7 x 10 -2mol / L; further comprising a reference particle, the reference particle being a silicone particle, the concentration of the silicone particle in the formed sample for detecting a formed element being in a range from 1.3 x 10 -2 g / L to 5.0 x 10 0 g / L; further comprising a preservative; the volume percentage of the preservative in the formed sample for detecting a formed element being 0.1%. As Figure 14 , some embodiments of the dry reagent for detecting a formed element further comprise glycerol; the concentration of the glycerol in the formed sample for detecting a formed element being in a range from 1.0 x 10 -1 mol / L to 8.1 x 10 -1 mol / L.

[0073] A method for preparing a formed element detection microscopy sample, for preparing a urine formed element detection microscopy sample, based on the dry reagent for detecting a formed element described above; comprising the following steps: C20: mixing the urine sample with the dry reagent for detecting a formed element to prepare a formed element detection microscopy sample.

[0074] As Figure 9 and Figure 11 , some embodiments of the dry reagent for detecting a formed element, for fecal formed element detection, the staining agent is new methylene blue, the concentration of the new methylene blue in the formed sample for detecting a formed element being in a range from 4.3 x 10 -4 mol / L to 1.7 x 10 -2 mol / L; further comprising an acid-base regulator, the acid-base regulator comprising disodium hydrogen phosphate and potassium dihydrogen phosphate; the concentration of the disodium hydrogen phosphate in the formed sample for detecting a formed element being in a range from 6.8 x 10 -2 mol / L to 1.3 x 10 -1 mol / L; the concentration of the potassium dihydrogen phosphate in the formed sample for detecting a formed element being in a range from 2.6 x 10 -2 mol / L to 4.7 x 10 -2 mol / L; further comprising barium sulfate, the concentration of the barium sulfate in the formed sample for detecting a formed element being in a range from 2.5 x 10 -1 mol / L to 3.9 x 10 0 mol / L; further comprising a preservative; the volume percentage of the preservative in the formed sample for detecting a formed element being 0.1%. As Figure 11 , some embodiments of the dry reagent for detecting a formed element further comprise glycerol; the concentration of the glycerol in the formed sample for detecting a formed element being in a range from 3.4 x 10 -1 mol / L to 2.7 x 10 -0 mol / L.

[0075] As Figure 10 and Figure 12The reconstitution reagent for reconstituting the dry reagent for detecting the formed elements comprises a fixing agent, and the concentration of the fixing agent in the microscopic sample for detecting the formed elements is 3.8*10 -3 mol / L to 5.8*10 -1 mol / L. Figure 10 The reconstitution reagent for reconstituting the dry reagent for detecting the formed elements comprises glycerol, and the concentration of the glycerol in the microscopic sample for detecting the formed elements is 3.4*10 -1 mol / L to 2.7*10 -0 mol / L.

[0076] The preparation method of the microscopic sample for detecting the formed elements is used for preparing the microscopic sample for detecting the formed elements in feces, is based on the dry reagent for detecting the formed elements and the reconstitution reagent, and comprises the following steps: B10, mixing the reconstitution reagent and the dry reagent for detecting the formed elements to form a staining solution; B20, adding the feces sample into the staining solution to prepare the microscopic sample for detecting the formed elements. Figure 11 The reconstitution reagent in the reconstitution reagent is matched with the formula in the formula. Figure 12 The reconstitution reagent in the reconstitution reagent is matched with the formula in the formula. Figure 9 The reconstitution reagent in the reconstitution reagent is matched with the formula in the formula. Figure 10 The reconstitution reagent in the reconstitution reagent is matched with the formula in the formula.

[0077] The present application is described and illustrated according to the preferred embodiments and several alternatives, but the application will not be limited by the specific description in the specification. Other additional alternatives or equivalent components can also be used to practice the present application.

Claims

1. A dry reagent for detecting formed elements, characterized in that, The formed element detection dry reagent is formed by drying a liquid staining reagent; The liquid staining reagent includes a staining agent and an acid-base adjuster; pH adjusters are used to adjust the pH value of liquid staining reagents; The liquid staining reagent is filtered before drying.

2. The dry reagent for detecting formed elements according to claim 1, characterized in that, Includes any one of the following technical features: TA1: Before drying, the liquid staining reagent is divided into small portions and dried separately; TA2: Divide the liquid staining reagent into single portions of the set volume, and then dry them to remove moisture.

3. The dry reagent for detecting formed elements according to claim 2, characterized in that, The capacity is set at 300ul-3000ul per vial.

4. The dry reagent for detecting formed elements according to claim 1, characterized in that, Includes any one of the following technical features: TB1: Filter the liquid staining reagent once using a 0.8μm filter head; TB2: For liquid staining reagents, first filter with a 0.8μm filter head, then filter a second time with a 0.22μm filter head; TN1: The staining agent is any one or more of methylene blue, neomethylene blue, brilliant tar blue, toluidine blue, neutral red, crystal violet, and methyl green; TN2: also includes reference particles; the reference particles are added to the liquid staining reagent after filtration. TN3: Also includes reference particles, which are organosilicon particles. In the liquid staining reagent, the concentration range of the organosilicon particles is 1.3 × 10⁻⁶. -2 g / L~5.0×10 0 g / L; TN4: Includes acid-base regulators, which are used to adjust the pH of microscopic samples for formed element detection to a weakly alkaline environment of 7-8; TN5 includes acid-base regulators, which include any one or more of Hepes-NaOH, Tris-HCl, MES-NaOH, phosphate buffer, phosphate buffer, and imidazole buffer. TN7: Also includes osmotic pressure regulating salts, which are used to adjust the microscopic samples for formed element detection to a low osmotic pressure environment; TN8: also includes osmotic pressure regulating salts, including sodium chloride; TN9 also includes dyeing accelerators, including EDTA salts; TN10: also includes preservatives, including preservative 950.

5. The dry reagent for detecting formed elements according to claim 1, characterized in that, Used for the detection of formed elements in blood. Includes any one of the following features: TXE1: The staining agent is neomethylene blue, and the concentration range of neomethylene blue in microscopic samples for formed element detection is 4.0 × 10⁻⁶. -3 g / L~5.0×10 -1 g / L; TXE2: The acid-base regulators include disodium hydrogen phosphate and potassium dihydrogen phosphate; the concentration range of said disodium hydrogen phosphate and potassium dihydrogen phosphate in microscopic samples for formed element detection is 2.0 × 10⁻⁶. -2 mol / L~1.0×10 -1 mol / L; TXE3: Also includes an osmotic pressure regulating salt, which includes sodium chloride; the concentration range of sodium chloride in microscopic samples for formed element detection is 2.2 × 10⁻⁶. -2 mol / L~3.5×10 -2 mol / L; TXE4: Also includes staining accelerators, including EDTA salts; the concentration range of EDTA salts in microscopic samples for formed element detection is 2.0 × 10⁻⁶. -4 mol / L~2.0×10 -3 mol / L; TXE5: also includes preservatives; in microscopic samples with formed elements, the volume percentage of preservatives is 0.1%; TXE6: Also includes glycerol; the concentration range of glycerol in microscopic samples for formed element detection is 1.0 × 10⁻⁶. -1 mol / L~8.1×10 -1 mol / L; TXE7: The staining agent is crystal violet, and the concentration range of crystal violet in microscopic samples for formed element detection is 6.2 × 10⁻⁶. -2 g / L~1.7×10 -0 g / L; TXE8: The staining agent is methylene blue, and the concentration range of methylene blue in microscopic samples for formed element detection is 7.4 × 10⁻⁶. -2 g / L~9.8×10 -1 g / L.

6. The dry reagent for detecting formed elements according to claim 1, characterized in that, Used for the detection of formed elements in urine. Includes any one of the following features: TNE1: The staining agent is neomethylene blue, and the concentration range of neomethylene blue in microscopic samples for formed element detection is 7.2 × 10⁻⁶. -4 mol / L~2.9×10 -2 mol / L; TNE2: Also includes acid-base regulators, including disodium hydrogen phosphate and potassium dihydrogen phosphate; the concentration range of disodium hydrogen phosphate in microscopic samples for formed element detection is 8.4 × 10⁻⁶. -2 mol / L~1.6×10 -1 The concentration range of potassium dihydrogen phosphate in microscopic samples for formed element detection is 2.0 × 10⁻⁶ mol / L. -2 mol / L~3.7×10 -2 mol / L; TNE3: Also includes reference particles, which are organosilicon particles. In microscopic samples with formed elements detected, the concentration range of organosilicon particles is 1.3 × 10⁻⁶. -2 g / L~5.0×10 0 g / L; TNE4: also includes preservatives; in microscopic samples with formed elements detected, the volume percentage of preservatives is 0.1%; TNE5: Also includes glycerol; the concentration range of glycerol in microscopic samples for formed element detection is 1.0 × 10⁻⁶. -1 mol / L~8.1×10 -1 mol / L.

7. The dry reagent for detecting formed elements according to claim 1, characterized in that, Used for the detection of formed elements in feces. Includes any one of the following features: TFE1: The staining agent is neomethylene blue, and the concentration range of neomethylene blue in microscopic samples for formed element detection is 4.3 × 10⁻⁶. -4 mol / L~1.7×10 -2 mol / L; TFE2: Also includes acid-base regulators, including disodium hydrogen phosphate and potassium dihydrogen phosphate; the concentration range of disodium hydrogen phosphate in microscopic samples for formed element detection is 6.8 × 10⁻⁶. -2 mol / L~1.3×10 -1 The concentration range of potassium dihydrogen phosphate in microscopic samples for formed element detection is 2.6 × 10⁻⁶ mol / L. -2 mol / L~4.7×10 -2 mol / L; TFE3: Also includes barium sulfate. In microscopic samples with formed elements, the concentration range of barium sulfate is 2.5 × 10⁻⁶. - 1 mol / L~3.9×10 0 mol / L; TFE4: also includes preservatives; in microscopic samples with formed elements, the volume percentage of preservatives is 0.1%; TFE5: Also includes glycerol; the concentration range of glycerol in microscopic samples for formed element detection is 3.4 × 10⁻⁶. -1 mol / L~2.7×10 -0 mol / L.

8. A method for preparing microscopic samples for the detection of formed elements, characterized in that, The dry reagent for detecting formed elements according to any one of claims 1 to 8.

9. A reconstitution reagent for reconstitution of dry reagents used in the detection of formed elements, characterized in that, Including fixatives; the concentration range of the fixative in microscopic samples for formed element detection is 1.4 × 10⁻⁶. -3 mol / L~5.3×10 -2 mol / L.

10. A method for preparing microscopic samples for the detection of formed elements, characterized in that, Used for preparing microscopic samples for the detection of formed elements in blood. Based on the dry reagent for detecting formed elements as described in claim 6; Based on the reconstitution reagent according to claim 10; Includes the following steps: A10: The reconstitution reagent is mixed with the dry reagent for detecting formed elements to form a staining solution; A20: Add the blood sample to the staining solution to prepare a sample for microscopic examination of the formed elements.

11. A reconstitution reagent for the reconstitution of dry reagents used in the detection of formed elements, characterized in that, Including fixatives; the concentration range of the fixative in microscopic samples for formed element detection is 3.8 × 10⁻⁶. -3 mol / L~5.8×10 -1 mol / L; Including glycerol; the concentration range of glycerol in microscopic samples for formed element detection is 3.4 × 10⁻⁶. -1 mol / L~2.7×10 - 0 mol / L.

12. A method for preparing microscopic samples for the detection of formed elements, characterized in that, Used for preparing microscopic samples for fecal formation analysis. Based on the dry reagent for detecting formed elements as described in claim 8; Based on the reconstitution reagent according to claim 12; Includes the following steps: B10: The reconstitution reagent is mixed with the dry reagent for detecting formed elements to form a staining solution; B20: Add the fecal sample to the staining solution to prepare a sample for microscopic examination of the formed elements.

13. A method for preparing microscopic samples for the detection of formed elements, characterized in that, Used for preparing urine samples for microscopic examination of formed elements. Based on the dry reagent for detecting formed elements as described in claim 7; Includes the following steps: C20: Mix the urine sample with the dry reagent for formed element detection to prepare a microscopic sample for formed element detection.

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