Refrigerating and asynchronous uniform mixing device for subpackaging cell sap

Abstract

The invention belongs to the technical field of medical instruments, and particularly relates to a refrigerating and asynchronous uniform mixing device for cell sap subpackaging. The device comprises a motor driving assembly, a first uniform mixing assembly, a second uniform mixing assembly, a hook assembly, a supporting plate and a refrigeration assembly. The hook assembly is installed on the supporting plate and used for hanging a cell sap bag. The first uniform mixing assembly and the second uniform mixing assembly are mounted on the supporting plate through a linear bearing assembly and are driven by the same motor driving assembly through a synchronous belt. Wherein the first uniform mixing assembly is fixed to one side of the synchronous belt, and the second uniform mixing assembly is fixed to the other side of the synchronous belt, so that when the motor is driven, the two uniform mixing assemblies can alternately move in a reciprocating mode, the cell sap bags are alternately extruded, and asynchronous periodic uniform mixing of the cell sap is achieved. The cell sap mixing device is compact in structure, double-component alternating action is achieved through a single motor, a multidirectional disturbance flow field is formed, mixing dead angles are effectively eliminated, the mixing efficiency and uniformity are remarkably improved, and the high requirement of the cell sap subpackaging technology is met.

Description

Technical Field

[0001] This invention belongs to the field of medical device technology, and in particular relates to a mixing device for cell fluid dispensing and a cell fluid dispensing device including the device. Background Technology

[0002] Cell therapy technology treats diseases by transplanting or introducing normal or bioengineered human cells into a patient's body to replace or repair damaged cells or stimulate and regulate the immune system. Based on cell type, current mainstream cell therapy technologies mainly include immune cell therapy (such as targeted dendritic cell therapy (T-DC), CIK cell therapy, and NK cell therapy) and stem cell therapy (which shows broad application prospects in cancer, viral diseases, hematological diseases, cardiovascular diseases, diabetes, Alzheimer's disease, and anti-aging treatments).

[0003] In clinical applications, cell solutions are typically stored initially in large-capacity soft bags and then aliquoted into several smaller bags as needed. To ensure the activity and homogeneity of each bag of cell solution after aliquoting, the cell solution in the large bag must be thoroughly mixed before aliquoting. However, current technologies commonly use manual shaking or a single motor driving a single baffle or two baffles oscillating synchronously for mixing, which has the following drawbacks: 1. Manual mixing is inefficient, labor-intensive, and difficult to guarantee uniformity.

[0004] 2. In the single-baffle or double-baffle synchronous mixing mode, the oscillation trajectory is fixed, and the forced flow direction of cell fluid is singular, which easily forms flow dead zones, resulting in insufficient mixing.

[0005] 3. Synchronous oscillation cannot fully utilize the inertial convection effect, resulting in low energy utilization and long mixing time.

[0006] 4. In a single-sided cooling scheme, the contact area of ​​the cooling plate is limited, resulting in a significant reduction in cooling efficiency.

[0007] To address the aforementioned problems, this invention proposes a mixing scheme driven by a single motor and using synchronous belt transmission to achieve alternating reciprocating motion of two mixing components, while simultaneously implementing dual-sided cooling. This scheme, while maintaining structural simplicity and reliable control, enables the cell fluid to form a multi-directional, periodic perturbation flow field under alternating propulsion, effectively eliminating flow dead zones and significantly improving mixing efficiency and uniformity. The cooling scheme employs dual-sided cooling, with one side using a cooling plate and the other side using cold air, thereby better meeting the stringent requirements for uniformity and temperature control in the cell fluid dispensing process. Summary of the Invention

[0008] The technical problem to be solved by the present invention is that the existing technology of cell fluid bag mixing has the problems of low efficiency, insufficient mixing and easy generation of dead corners. The present invention provides a refrigeration and asynchronous mixing device and cell fluid dispensing equipment for cell fluid dispensing.

[0009] To address the aforementioned problems, this invention provides a refrigeration and asynchronous mixing device for dispensing cell fluid, comprising a motor drive assembly, a hook assembly, a first mixing assembly, a second mixing assembly, a support plate, and a refrigeration assembly mounted on the support plate; the hook assembly is mounted on the support plate for attaching cell fluid bags; the first and second mixing assemblies are mounted on the support plate via linear bearing assemblies; the motor drive assembly includes a motor and a transmission assembly, and when the motor drive assembly drives the transmission assembly, the first and second mixing assemblies can alternately reciprocate, thereby alternately squeezing the cell fluid bags attached to the hook assembly.

[0010] Furthermore, both the first mixing component and the second mixing component are driven by the same motor drive component.

[0011] Furthermore, the transmission assembly includes a timing belt, with the first mixing assembly fixed to one side of the timing belt and the second mixing assembly fixed to the other side of the timing belt.

[0012] Furthermore, the motor drive assembly includes a driving wheel and a driven wheel, and a timing belt is sleeved between the driving wheel and the driven wheel. The first mixing assembly is fixed to one side of the timing belt, and the second mixing assembly is fixed to the opposite side of the timing belt. The timing belt drives the first mixing assembly and the second mixing assembly to reciprocate alternately.

[0013] Furthermore, the two mixing components are arranged vertically.

[0014] Furthermore, the motor drive assembly also includes a reduction mechanism for adjusting the operating speed of the transmission structure, thereby adjusting the movement speed of the mixing assembly.

[0015] Furthermore, both the first mixing assembly and the second mixing assembly include a mixing plate, a connecting plate, and a linear bearing; the linear bearing is fixed to the support plate; the mixing plate is fixed to one end of the linear bearing, the connecting plate is fixed to the other end of the linear bearing, and the connecting plate is fixedly connected to the transmission structure.

[0016] Furthermore, the mixing mechanism can adjust the mixing amplitude through software parameters according to the change in the amount of liquid in the cell sap bag, so as to meet the mixing effect when the amount of liquid is small.

[0017] Furthermore, the mixing plate is provided with a clearance groove for avoiding the cell fluid bag tubing.

[0018] Furthermore, the refrigeration component is a compressor refrigeration component, which is fixed to the back plate of the support plate.

[0019] Furthermore, the cell fluid bag adopts a two-layer structure. The inner layer has a cell fluid storage function, and the outer layer is cooled by a refrigeration component on the side close to the support plate. The outer layer is provided with a cooling channel on the side away from the support plate. This channel is used to connect the refrigerant, thereby realizing double-sided cooling of the cell fluid bag.

[0020] A cell fluid dispensing device, characterized in that it includes a housing and a refrigeration and asynchronous mixing device for cell fluid dispensing as described in any one of claims 1-10, wherein the asynchronous mixing device is installed in the housing.

[0021] This invention improves the thoroughness and efficiency of mixing by alternating the action of two components, creating bidirectional perturbation within one motion cycle, and effectively preventing cell deposition.

[0022] This invention introduces cold air by adding ventilation channels through cell fluid bags, while the rear cooling plate simultaneously cools the air, achieving cooling from both sides. This can improve cooling efficiency and reduce cooling time.

[0023] The present invention maintains a good low-temperature environment during cell filling, which has the following beneficial effects: 1. Ensuring cell viability and functional integrity. Cells are highly sensitive to temperature changes; at room temperature, they are prone to disruption, decreased activity, and even apoptosis. Low temperatures can significantly slow down cellular metabolic rates, reduce energy consumption, thereby prolonging cell survival time and maintaining their biological functions. 2. Reduce toxicity. Cells are resuspended in cryopreservation solution and then filled. The cryopreservation solution contains components such as DMSO, which are toxic to cells at room temperature. The low temperature environment can reduce this toxic effect.

[0024] 3. Low temperature environment helps cryopreservation solution to perform better. The cryoprotectant in the cryopreservation solution (such as DMSO) combines with water molecules, lowers the freezing point, reduces the formation of ice crystals, and thus avoids the damage of ice crystals to cell membranes.

[0025] 4. Under low temperature conditions, the permeability of cell membranes changes, making it easier for cryopreservation agents to penetrate into the cell and combine with intracellular water to form a protective barrier, preventing cell damage during subsequent freezing. Attached Figure Description

[0026] Figure 1 is a schematic diagram of the asynchronous mixing device provided in an embodiment of the present invention; Figure 2 is an exploded structural diagram of the asynchronous mixing device provided in an embodiment of the present invention; Figure 3 is a structural schematic diagram of another embodiment of the present invention; Figure 4 is a schematic diagram of the exploded structure of another embodiment of the present invention; Figure 5 is a schematic diagram of the cell fluid bag of the present invention.

[0027] The following are the reference numerals in the accompanying drawings: 1. Hook assembly; 2. Cell fluid bag; 3. First mixing assembly; 31. First mixing plate; 32. First mixing assembly connecting plate; 33. Linear bearing assembly; 4. Second mixing assembly; 41. Second mixing plate; 42. Second mixing assembly connecting plate; 43. Mixing assembly connecting block; 5. Support plate; 6. Motor drive assembly; 61. Driven wheel / driven wheel; 62. Synchronous belt; 63. Motor drive assembly fixing plate; 64. Driven wheel shaft; 65. Bearing seat; 66. Motor; 67. Reduction mechanism; 7. Refrigeration assembly; 8. Cold air connector. Detailed Implementation

[0028] To make the technical problems solved, the technical solutions, and the beneficial effects of this invention clearer, the invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only for explaining the invention and are not intended to limit the invention.

[0029] It should be understood that the terms "upper", "lower", "left", "right", "front", "rear", "middle", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations of the present invention.

[0030] As shown in Figures 1 and 3, an asynchronous mixing device provided in this embodiment of the invention includes a hook assembly 1, a first mixing assembly 3, a second mixing assembly 4, a support plate 5, a motor drive assembly 6, and a cooling assembly 7. The hook assembly 1 is mounted on the upper part of the support plate 5 and is used to hang a cell fluid bag 2. The upper part of the cell fluid bag 2 is hooked onto the hook assembly 1, and the lower part is located between the first mixing assembly 3, the second mixing assembly 4, and the support plate 5. The first mixing assembly 3 and the second mixing assembly 4 are mounted on the support plate 5 and are both connected to the motor drive assembly 6. The motor drive assembly 6 is used to drive the first mixing assembly 3 and the second mixing assembly 4 to move, causing them to squeeze the cell fluid in the cell fluid bag 2.

[0031] As shown in Figure 4, each mixing assembly includes a mixing plate 32 or 42, a connecting plate 31 or 41, and a linear bearing assembly 33. Linear bearing assemblies 33 and 43 are fixed to the support plate 5, perpendicular to the support plate 5 and penetrating its plane. The mixing plate and connecting plate are fixed to both ends of the linear bearing, and the connecting plate is then fixedly connected to the timing belt to ensure smooth movement.

[0032] As shown in Figures 1 and 4, the first mixing component 3 and the second mixing component 4 are fixed to the synchronous belt 62 of the motor drive component 6 via their connecting plates (32, 42). The mixing amplitude can be manually or automatically adjusted according to the liquid volume, using software parameters to adapt to different cell fluid volumes. For example, the fixed position of the connecting plate on the synchronous belt can be manually adjusted, or the adjustment can be automatically achieved through automated software parameters based on the remaining liquid volume collected by the liquid volume acquisition device. The motor drive component 6 drives the synchronous belt 62 in a cyclical motion. Since the first mixing component 3 and the second mixing component 4 are fixed on opposite sides of the synchronous belt, when the synchronous belt moves, the two components generate reciprocating motions in opposite directions, thereby alternately squeezing the cell fluid bag 2 and driving the cell fluid to form an alternating bidirectional flow, achieving efficient and thorough mixing. This device achieves automatic mixing, improves mixing efficiency and quality, and has a simple structure and low cost.

[0033] like Figure 1 As shown, hook assembly 1 can be manually replaced. For cell culture bags 2 of different volumes, different length hook assemblies can be used to adapt the device, improving its versatility.

[0034] As shown in Figure 1, the bottom of the second mixing plate 41 is provided with a clearance groove. During the reciprocating compression process, the groove can avoid the tubing structure at the bottom of the cell fluid bag, avoid interference, ensure that the cell fluid at the bottom of the bag can also be fully mixed, and prevent sedimentation.

[0035] As shown in Figure 4, the motor drive assembly 6 includes a motor 66, a driving wheel, a driven wheel, a synchronous belt 62, and a motor drive assembly fixing plate 63. The motor 66 is mounted on the fixing plate 63 and connected to the driving wheel. The motor 66 drives the driving wheel to rotate, and the driving wheel drives the driven wheel to rotate via the synchronous belt 62, thereby driving the synchronous belt to circulate, which in turn drives the first mixing assembly 3 and the second mixing assembly 4 to reciprocate alternately. In this embodiment, the motor 66 and the cell fluid bag 2 are located on opposite sides of the support plate 5, resulting in a compact structure.

[0036] In addition to the belt drive structure described above, it is conceivable that a gear and rack transmission method could also be used to drive the first mixing component 3 and the second mixing component 4 to reciprocate alternately. The rotation of the gear simultaneously drives two racks moving in different directions, thus driving the first mixing component 3 and the second mixing component 4 to reciprocate alternately.

[0037] In addition to belt drive and rack and pinion drive, various other transmission methods can be used to form a transmission assembly to achieve asynchronous transmission between the first mixing assembly 3 and the second mixing assembly 4.

[0038] As shown in Figure 3, the cooling component 7 is fixed to the back plate of the support plate 5. This embodiment uses a compressor cooling method, which, compared with traditional Peltier cooling, eliminates the need for complex fan cooling and air duct design, resulting in higher cooling efficiency and faster cooling of cell fluid to the required temperature, thus helping to maintain cell viability.

[0039] As shown in Figure 2, the motor drive assembly 6 also includes a reduction gear mechanism. Since cell fluid mixing requires strict speed control (excessive speed can easily lead to cell rupture), and stepper motors are prone to vibration at low speeds, adding a reduction gear mechanism can ensure smooth motor operation, eliminate low-frequency vibrations, and achieve fine adjustment of the mixing speed to meet the needs of different cell types and processes.

[0040] As shown in Figure 5, the cell sap bag consists of two layers, an inner and an outer layer. The inner layer of the cell sap bag body 22 serves as a cell sap storage layer, while the outer layer, on the side away from the support plate, has a cold air channel 21 for connecting to the cold air from the rear compressor, thus achieving a dual-sided cooling system. This channel 21 can also be configured as a cooling channel for other refrigerants.

[0041] like Figure 1 , Figure 5 As shown, the cold air connector 8 is located on the cooling plate and connects to the cell fluid bag via a quick-connect method to achieve cold air circulation. It can be understood that before the cell fluid bag is mixed, the cold air connector is connected to the cell fluid bag connector to connect the cold air generated by the cooling component 7 to the air passage of the cell fluid bag 2 to achieve front-side cooling.

[0042] Another embodiment of the present invention provides a cell fluid dispensing device, including a housing (not shown in the figure) and the above-mentioned asynchronous mixing device, which is installed in the housing and integrated into the complete dispensing process.

[0043] The above are merely preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A refrigeration and asynchronous mixing device for dispensing cell fluid, characterized in that, The device includes a motor drive assembly, a hook assembly, a first mixing assembly, a second mixing assembly, a support plate, and a cooling assembly mounted on the support plate. The hook assembly is mounted on the support plate for attaching cell fluid bags. The first and second mixing assemblies are mounted on the support plate via linear bearing assemblies. The motor drive assembly includes a motor and a transmission assembly. When the motor drive assembly drives the transmission assembly, the first and second mixing assemblies can reciprocate alternately, thereby alternately squeezing the cell fluid bags attached to the hook assembly.

2. The refrigeration and asynchronous mixing device for cell fluid dispensing according to claim 1, characterized in that, Both the first mixing component and the second mixing component are driven by the same motor drive component.

3. The refrigeration and asynchronous mixing apparatus for cell fluid dispensing according to claim 1 or 2, characterized in that, The transmission assembly includes a timing belt, the first mixing component is fixed to one side of the timing belt, and the second mixing component is fixed to the other side of the timing belt.

4. The refrigeration and asynchronous mixing device for cell fluid dispensing according to claim 3, characterized in that, The motor drive assembly includes a driving wheel and a driven wheel. A synchronous belt is sleeved between the driving wheel and the driven wheel. The first mixing assembly is fixed to one side of the synchronous belt, and the second mixing assembly is fixed to the opposite side of the synchronous belt. The synchronous belt drives the first mixing assembly and the second mixing assembly to reciprocate alternately.

5. The refrigeration and asynchronous mixing device for cell fluid dispensing according to claim 1, characterized in that, The two mixing components are arranged vertically.

6. The refrigeration and asynchronous mixing device for cell fluid dispensing according to claim 1, characterized in that, The motor drive assembly also includes a reduction mechanism for adjusting the operating speed of the transmission structure, thereby adjusting the movement speed of the mixing assembly.

7. The refrigeration and asynchronous mixing device for cell fluid dispensing according to claim 1, characterized in that, The mixing mechanism can adjust the mixing amplitude manually or automatically via software parameters according to changes in the amount of liquid in the cell sap, thus achieving the desired mixing effect even with a small amount of liquid.

8. The refrigeration and asynchronous mixing device for cell fluid dispensing according to claim 1, characterized in that, Both the first mixing assembly and the second mixing assembly include a mixing plate, a connecting plate, and a linear bearing; the linear bearing is fixed to the support plate; the mixing plate is fixed to one end of the linear bearing, the connecting plate is fixed to the other end of the linear bearing, and the connecting plate is fixedly connected to the transmission structure.

9. The refrigeration and asynchronous mixing device for cell fluid dispensing according to claim 1, characterized in that, The mixing plate is provided with clearance grooves to avoid the cell fluid bag tubing.

10. The refrigeration and asynchronous mixing apparatus for cell fluid dispensing according to claim 1, characterized in that, The refrigeration component is a compressor refrigeration component, which is fixed to the back plate of the support plate.

11. The refrigeration and asynchronous mixing device for cell fluid dispensing according to claim 1, characterized in that, The cell fluid bag has a two-layer structure. The inner layer has a cell fluid storage function, and the outer layer is cooled by a refrigeration component on the side close to the support plate. The outer layer is provided with a cooling channel on the side away from the support plate. This channel is used to connect the refrigerant, thereby realizing double-sided cooling of the cell fluid bag.

12. The refrigeration and asynchronous mixing device for cell fluid dispensing according to claim 1, characterized in that, The transmission component is specifically a belt drive or a rack and pinion drive.

13. The refrigeration and asynchronous mixing apparatus for dispensing cell fluid according to claim 11, characterized in that... One refrigerant from the compressor is introduced into the cooling channel of the mixing bag, and the other is introduced into the cooling plate on the back of the mixing bag, thus completing the cooling of both sides of the mixing bag.

14. The refrigeration and asynchronous mixing apparatus for dispensing cell fluid according to claim 11, characterized in that: The cooling channel can be detachably or fixedly attached to the bag body.

15. A cell fluid dispensing device, characterized in that, It includes a housing and a refrigeration and asynchronous mixing device for dispensing cell fluid as described in any one of claims 1-10, wherein the asynchronous mixing device is installed inside the housing.

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