A monoclonal antibody specifically recognizing sarcocystis hominis cyst and oocyst and sporocyst and its application

By developing a monoclonal antibody 5H2 that specifically identifies Sarcocystis sheep and an ELISA kit, the problem of low sensitivity in existing detection methods has been solved, achieving highly sensitive detection of Sarcocystis sheep cysts and oocysts, thus improving detection efficiency and accuracy.

CN121736099BActive Publication Date: 2026-07-10SHANGHAI VETERINARY RESEARCH INSTITUTE CAAS (CHINESE ANIMAL HEALTH & EPIDEMIOLOGY CENTER SHANGHAI BRANCH) +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SHANGHAI VETERINARY RESEARCH INSTITUTE CAAS (CHINESE ANIMAL HEALTH & EPIDEMIOLOGY CENTER SHANGHAI BRANCH)
Filing Date
2026-02-03
Publication Date
2026-07-10

AI Technical Summary

Technical Problem

Existing methods for detecting Sarcocystis are not sensitive enough and are prone to false negatives. Furthermore, immunological assays lack suitable antigens that can detect both cysts and oocysts simultaneously, making it difficult to detect them effectively in intermediate and definitive hosts.

Method used

A monoclonal antibody 5H2 specifically recognizing Sarcocystis sheepii was developed, and an ELISA kit containing this antibody was prepared for detecting Sarcocystis sheepii cysts and oocysts. Through a series of steps including antigen coating, blocking, serum incubation, monoclonal antibody incubation, and enzyme-labeled secondary antibody incubation, highly sensitive detection of Sarcocystis sheepii was achieved.

Benefits of technology

It significantly improves the detection rate of clinical samples, advances the detection window by 7-10 days, is simple to operate, is suitable for live animal testing, and improves testing efficiency.

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Abstract

The application provides a monoclonal antibody specifically recognizing a cyst and an egg / sporocyst of a sheep trichophyton and application thereof, the provided monoclonal antibody can positively react to a soluble antigen of a whole protein of a trichophyton adult and a soluble antigen of an egg / sporocyst, and simultaneously covers two key development stages of the adult and the egg / sporocyst. A complete parasite life cycle detection system can be established, and a clinical sample detection rate is significantly improved (experiments show that the detection rate is improved by 35-40%). Moreover, a detection window period is 7-10 days earlier than that of a traditional method. Meanwhile, the application provides an ELISA detection kit and a detection method thereof, the ELISA detection kit and the detection method thereof are high in sensitivity, convenient to operate, can greatly improve detection efficiency, and have a good industrial application prospect.
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