Application of beta-phellandrene in luring fly organisms

Abstract

The invention relates to application of beta-phellandrene in fly biological luring, which takes beta-phellandrene as an active component of a fly biological attractant, and can realize luring, monitoring or trapping and killing fly adults in the environments of agricultural production areas, livestock and poultry farms, fruit and vegetable processing and storage places, garbage treatment and composting places, urban and rural environmental health prevention and control and the like. The prevention and control effect is greatly improved, the use of chemical insecticides can be reduced or replaced, and the green prevention and control development direction is met.

Description

Technical Field

[0001] This invention belongs to the field of biotechnology and relates to the application of β-phellandrene in attracting flies. Background Technology

[0002] Flies (Diptera) are a group of highly adaptable and reproductive pests, widely found in agricultural production areas, livestock farms, fruit and vegetable processing and storage facilities, and urban and rural living environments. Common flies such as houseflies, fruit flies, and blowflies not only directly contaminate food and agricultural products but also serve as vectors for various pathogenic microorganisms, posing a serious threat to human health, livestock safety, and agricultural ecosystems.

[0003] Currently, fly pest control mainly relies on chemical pesticides, poison baits, or chemical attractants. However, these methods have the following shortcomings in practical application: (1) Long-term use can easily induce drug resistance in flies; (2) Drug residues have adverse effects on the environment and non-target organisms; (3) Some attractants have poor selectivity, high trapping rate of miscellaneous insects, and limited control efficiency.

[0004] Therefore, developing a novel attractant that is naturally derived, environmentally friendly, and has good selectivity and attraction effects on flies is a technical problem that urgently needs to be solved in the field of green control of fly pests.

[0005] CN112485414A discloses that β-phellandrene, a volatile compound derived from sunflower, has a repellent effect against the peach fruit borer at a concentration of 0.01 v / V, and can be used as a repellent for the peach fruit borer. The peach fruit borer (Conogethes punctiferalis Guenée) belongs to the order Lepidoptera and family Pyralidae. Its larvae bore into the fruits of chestnuts, peaches, sunflowers, and other plants, making it an important and difficult-to-control borer pest. β-phellandrene is a monoterpenoid volatile compound widely found in various aromatic plants, possessing strong diffusion ability and a stable odor characteristic.

[0006] Although plant volatile organic compounds (VOCs) are based on insect olfactory recognition and chemotaxis, there are significant differences in the intrinsic stimuli, functional properties of VOCs, insect behavioral logic, and physiological mechanisms of response among different insects. The peach fruit borer exhibits innate phytotaxis based on the specific chemical signals of its host plant, requiring screening for characteristic VOCs from its host plant, primarily terpenoids, alcohols, and esters. In contrast, some flies exhibit saprophytic chemotaxis based on non-specific plant volatile signals simulating saprophytic substrates, constituting a "signal misinterpretation" and having no specific association with plants.

[0007] This invention aims to further reveal the trapping effect of sunflower volatile compound β-phellandrene on flies. Summary of the Invention

[0008] The purpose of this invention is to overcome the shortcomings of the existing technology and provide an application of β-phellandrene in fly bioattraction.

[0009] The objective of this invention can be achieved through the following technical solutions: The technical solution of the present invention is to provide the application of β-phellandrene in the preparation of fly biological attractants.

[0010] In some specific embodiments, the fly attractant is composed of either 1) or 2) as follows: 1) β-Phasene; 2) β-phellandrene and at least one co-attractant, wherein the co-attractant is selected from one or more plant-derived volatiles, food-derived volatiles, or fly pheromones.

[0011] In some specific embodiments, the co-attractant is selected from any one or more of 3-methylene-6-(tert-pentyl)cyclohex-1-ene, 4-oxoisophorone, and 3-carene.

[0012] In some specific embodiments, the fly attractant may be in the form of a liquid or a powder.

[0013] In some specific embodiments, when the fly attractant is a liquid agent, the solvent of the liquid agent is n-hexane, and the concentration of β-phellandrene used is 1 μg / μL to 100 μg / μL.

[0014] In some specific embodiments, when the fly attractant is a powder, the mass of β-phellandrene used is 0.1~10 mg.

[0015] In some specific embodiments, when the fly attractant is a powder, the amount of 3-carene used in the synergistic attractant is 0.1 mg, and / or the amount of 4-oxoisophorone is 10 mg, and / or the amount of 3-methylene-6-(tert-amyl)cyclohex-1-ene is 10 mg.

[0016] In some specific embodiments, when the fly attractant is applied, the fly attractant is loaded into a slow-release carrier and used in combination with a trap, the traps being spaced 20 m apart and suspended on plants at a height of 1 m above the ground.

[0017] In some specific embodiments, the sustained-release carrier is selected from any one of plastic capillary tubes, rubber stoppers, polymer sustained-release tubes, microporous polyethylene tubes, paraffin-based, and polymer-based sustained-release matrices.

[0018] In some specific embodiments, the trap is a boat-shaped trap.

[0019] Compared with the prior art, the present invention has the following advantages: (1) The present invention uses plant-derived volatile β-phellandrene as the core active ingredient. It is safe and environmentally friendly, and can attract, monitor or kill adult flies in agricultural production areas, livestock and poultry farms, fruit and vegetable processing and storage sites, garbage disposal and composting sites, urban and rural environmental sanitation control and other environments, which greatly improves the control effect.

[0020] (2) The fly biological attractant provided by the present invention has a significant attraction effect on adult flies and can be used for population monitoring and early warning.

[0021] (3) The present invention improves the attraction stability and persistence of fly biological attractants through the design of a slow-release system.

[0022] (4) This invention expands the range of insects that can be trapped by the plant-derived volatile compound β-phellandrene, which can reduce or replace the use of chemical pesticides and is in line with the development direction of green pest control. Attached Figure Description

[0023] Figure 1 A statistical chart showing the number of flies captured by different biological attractants. Detailed Implementation

[0024] The present invention will now be described in detail with reference to the accompanying drawings and specific embodiments. These embodiments are based on the technical solution of the present invention and provide detailed implementation methods and specific operating procedures. However, the scope of protection of the present invention is not limited to the following embodiments.

[0025] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the application.

[0026] Unless otherwise specified, the materials and processes used in the following embodiments or examples are conventional materials and processes employed in the art to achieve the corresponding functions.

[0027] Example 1: This embodiment provides a method for preparing a liquid fly attractant and its application. The preparation method includes the following steps: (1) Dilution: Using hexane as solvent, β-phellandrene, 3-methylene-6-(tert-pentyl)cyclohex-1-ene, 1-isopropyl-4-methylenecyclohexane, ethyl 4-methylenecyclohexane-1-carboxylate, 4-oxoisophorone, and 3-carene were diluted to 1 μg / μL, 10 μg / μL, and 100 μg / μL of mother liquor, shaken to mix well, and placed in a -20℃ refrigerator for later use.

[0028] (2) Loading: Using a plastic capillary tube as a carrier, fill 100 μL of the compound in step (1) to obtain the fly biological attractant to be tested.

[0029] (3) Combination: Combine the white sticky board with the boat-shaped trap, attach the fly attractant from step (2) to the trap, and place it in the peach orchard.

[0030] Example 2: This embodiment tests the performance of the various fly attractants in Example 1. From June 9 to June 29, 2025, three experimental plots were selected in the peach orchard of the Huangtao Picking and Fishing Center in Fengxian District, Shanghai, China (30°09′N, 121°05′E) for a lure validation experiment. Individual lures (fly biological attractants) were tied to the top of boat-shaped traps (Beijing Zhongjie Sifang Biotechnology Co., Ltd., Beijing, China). The traps were suspended 1 m above the ground on peach trees, with each trap spaced 20 m apart. A randomized complete block design was used, repeated 6 times. The number of adult flies captured by each trap was recorded. Lures filled with hexane were used as a control.

[0031] A total of 4014 flies and other insects of various species were captured in this experiment, as shown in Table 1 and [Table data would be inserted here]. Figure 1 The addition of 0.1, 1, and 10 mg of β-phellandrene, 10 mg of 3-methylene-6-(tert-amyl)cyclohex-1-ene, 10 mg of 4-oxoisophorone, and 0.1 mg of 3-carene showed highly significant differences (P < 0.01) compared to the control (average hexane trapping of 16 insects). This is because the increased number of attracted adults leads to a decrease in their mating rate, thereby reducing the reproductive rate and ultimately reducing the degree of damage.

[0032] Table 1 The above description of the embodiments is provided to enable those skilled in the art to understand and use the invention. It will be apparent to those skilled in the art that various modifications can be made to these embodiments, and the general principles described herein can be applied to other embodiments without inventive effort. Therefore, the present invention is not limited to the above embodiments, and any improvements and modifications made by those skilled in the art based on the disclosure of the present invention without departing from the scope of the invention should be within the protection scope of the present invention.

Claims

1. Use of beta-phellandrene in the preparation of a fly biological attractant.

2. Use according to claim 1, characterized in that, The fly biological attractant is composed of 1) or 2) 1) beta-phellandrene; 2) beta-phellandrene and at least one synergistic attractant selected from one or more of plant-derived volatiles, food-derived volatiles or fly pheromones. The synergistic attractant is selected from any one or more of 3-methylene-6-(tert-pentyl)cyclohex-1-ene, 4-oxoisophorone, 3-carene.

3. Use according to claim 2, characterized in that, The form of the fly biological attractant includes liquid agent, powder agent.

4. Use according to claim 3, characterized in that, When the fly biological attractant is a liquid agent, the solvent of the liquid agent is n-hexane, and the use concentration of beta-phellandrene is 1 μg / μL~100 μg / μL.

5. Use according to claim 4, characterized in that, When the fly biological attractant is a powder agent, the use mass of beta-phellandrene is 0.1~10 mg.

6. Use according to claim 4, characterized in that, When the fly biological attractant is a powder agent, the use amount of 3-carene in the synergistic attractant is 0.1 mg, and / or the use amount of 4-oxoisophorone is 10 mg, and / or the use amount of 3-methylene-6-(tert-pentyl)cyclohex-1-ene is 10 mg.

7. Use according to claim 4, characterized in that, In the application of the fly biological attractant, the fly biological attractant is loaded in a slow-release carrier and used in combination with a trap, the distance between the traps is 20 m, and the traps are hung on plants at a height of 1 m from the ground.

8. The use according to claim 1, characterized in that, The slow-release carrier is selected from any one of plastic capillary tubes, rubber plugs, polymer slow-release tubes, microporous polyethylene tubes, paraffin-based, and polymer-based slow-release substrates.

9. Use according to claim 8, characterized in that, The trap is a boat-shaped trap.

10. Use according to claim 8, characterized in that, ​

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