Composite daphne giraldii seed treating fluid with epimedium polysaccharide as main effective component and application of composite daphne giraldii seed treating fluid

Abstract

The invention provides a daphne giraldii seed composite treatment solution with epimedium polysaccharide as a main effective component and application, and belongs to the technical field of plant seed treatment, the daphne giraldii seed composite treatment solution comprises 100-300 mg / L of gibberellin, 0.1-100 mg / L of epimedium polysaccharide, 15-35 mg / L of 6-benzylamino adenine, 50-150 mg / L of oligosaccharide, 1-30 mg / L of benzothiadiazole, 20-60 mg / L of a bactericide and 0-1 mL / L of a surfactant. According to the application, the daphne giraldii seeds are treated with the daphne giraldii seed composite treating fluid. According to the composite treatment liquid and the treatment method, the germination rate and the germination potential of the seeds can be remarkably improved, mildewing in the stratification period is effectively inhibited, the growth potential of seedlings is enhanced, a standardized and repeatable operation process is provided, and the composite treatment liquid and the treatment method are suitable for large-scale production.

Description

Technical Field

[0001] The present invention relates to the technical field of plant seed treatment, and particularly to a compound treatment solution for Daphne giraldii Nitsche seeds with epimedium polysaccharide as the main active ingredient and its application, specifically to a compound treatment solution and treatment method for breaking the dormancy of Daphne giraldii Nitsche seeds, improving the germination rate and enhancing the resistance of seedlings. Background Technique

[0002] Daphne giraldii Nitsche Daphne giraldii is a small shrub of the genus Daphne in the family Thymelaeaceae and is one of the original plants of the traditional Chinese medicine "Zushi Ma" in China, mainly distributed in high-altitude areas such as Gansu and Qinghai. In the wild state, it mainly relies on seed reproduction. However, due to the deep dormancy characteristics of seeds, the germination rate is extremely low under natural conditions, the germination cycle is long, and the seedling formation rate is not high, which severely restricts its artificial cultivation and resource restoration. During the wild and artificial cultivation of Daphne giraldii Nitsche, the seeds have an obvious compound dormancy mechanism, including seed coat restriction, physiological dormancy, and the influence of germination inhibitors.

[0003] At present, the artificial cultivation technology of Daphne giraldii Nitsche is still immature, and relevant basic research is relatively lacking. Regarding the seed dormancy problem, existing research mainly focuses on methods such as soaking seeds with plant hormones (such as gibberellin GA3 and 6-benzylaminopurine 6-BA), low-temperature stratification, concentrated sulfuric acid etching, sand storage treatment, and optimization of germination beds. For example, it has been reported that when soaking seeds with 200mg / L GA3 solution for 16h or 25mg / L 6-BA solution for 16h, the highest germination rates of Daphne giraldii Nitsche seeds are only 7.33% and 6.33% respectively, with relatively low germination rates. Another study showed that when soaking seeds with a mixture of 200mg / L GA3 and 25mg / L 6-BA for 10h and then combining with 70d low-temperature stratification treatment, the germination rate can reach 64.93%. However, this treatment is extremely sensitive to conditions and is prone to unstable germination rates due to operation or environmental fluctuations in actual applications; moreover, this study only uses the breakthrough of the radicle through the seed coat as the germination standard and does not track subsequent growth indicators of seedlings such as cotyledon expansion, true leaf growth, plant height, biomass, and transplanting survival rate. Therefore, there is a potential risk of poor development after emergence. Breaking seed dormancy is only the initial step, and the establishment of healthy seedlings is the key to successful cultivation.

[0004] Studies have reported that after 60 minutes of acid etching with concentrated sulfuric acid followed by 24 hours of soaking in 200 mg / L GA3, the germination rate was only 24.22%, which was still unsatisfactory. Another study comparing different dormancy-breaking methods found that deep stratification in sand for 170 days achieved the highest seedling survival rate (51.27%), but this treatment cycle was too long, costly, and inefficient, making it unsuitable for large-scale seedling production. Furthermore, this experiment was conducted in a greenhouse, and its applicability in field or different ecological regions was not verified, thus its promotional value is questionable. In contrast, the seedling survival rate after acid etching with concentrated sulfuric acid followed by hormone soaking was only 7.79%. Some studies exploring the time and depth of stratification indicated a higher germination rate when stratified on October 20th at a depth of 80 cm. However, this study only used germination rate as an evaluation indicator and did not examine indicators reflecting germination quality and seedling vigor, such as germination potential, germination index, seedling growth vigor, and root development, thus failing to ensure good field adaptability of the seedlings.

[0005] Studies have confirmed that low-temperature stratification for 125 days can achieve a germination rate of 33.64%. However, while treatment with GA3, 6-BA, and concentrated sulfuric acid can partially release dormancy, they fail to effectively break dormancy. Another study developed a hormone-combined low-temperature stratification method, using a mixture of 150 mg / L GA3 and 30 mg / L 6-BA for 10 hours followed by stratification for 70 days, achieving a germination rate of 82.6%, but with poor seedling emergence and seedling death. The study also indicated that outdoor natural stratification treatment resulted in an 85% seed cracking rate and an 84% seedling emergence rate, with stronger seedlings. Furthermore, indoor stratification treatment tended to lead to poor hypocotyl development and a lower seedling emergence rate. Although this study proposed both indoor hormone-combined stratification and outdoor natural stratification methods, it did not conduct long-term monitoring of seedling growth, stress resistance, and accumulation of medicinal components after seedling cultivation.

[0006] In summary, while existing methods can improve the germination rate of Daphne odora seeds to some extent and partially solve the problem of dormancy breaking, they still generally suffer from unstable germination rates, long treatment cycles, complex operations, high costs, and poor seedling development or low seedling survival rates after emergence. Therefore, there is an urgent need in this field for a comprehensive treatment scheme that can effectively break seed dormancy, improve germination uniformity, enhance seedling resistance, and is feasible. This will provide a theoretical basis for improving the efficiency of artificial seedling propagation of Daphne odora and promoting the restoration and standardized cultivation of wild populations.

[0007] Epimedium polysaccharides possess a variety of activities, including antiviral, antitumor, antioxidant, immunomodulatory, and anti-aging effects. They can effectively regulate immunity, fight viruses, slow aging, stimulate bone marrow DNA synthesis, promote platelet aggregation, and have antioxidant and hormone-enhancing effects. Modern pharmacological research and clinical applications show that, as an important immunostimulant, it can exert broad physiological activities by promoting dendritic cell maturation, activating thymic immune function, and enhancing cellular immune responses. Studies have shown that Epimedium polysaccharides can enhance the immune response to H1N1 in mice and promote DNA synthesis and hepatocyte proliferation. Other studies have indicated that Epimedium polysaccharides can enhance immune responses, promote the production of cytokines such as IL-2, activate the body's defense system, and stimulate bone marrow DNA synthesis and hepatocyte proliferation, demonstrating its activity in promoting cell division and metabolism.

[0008] Toxicological studies have shown that Epimedium polysaccharides do not possess mutagenic, teratogenic, or embryotoxic properties, exhibiting high safety. Furthermore, polysaccharides generally have better biocompatibility and milder effects, leading to their widespread application in animal and pharmaceutical fields with a high safety profile. Some patents explicitly list Epimedium polysaccharides alongside Astragalus polysaccharides and Lycium barbarum polysaccharides as a class of bioactive polysaccharides with immune-enhancing effects, used to construct sustained-release systems and enhance the body's resistance. Other studies, when describing compositions for stabilizing bioactive materials, list Epimedium polysaccharides as one of many bioactive polysaccharides, emphasizing their potential in stabilizing and protecting bioactive substances. Summary of the Invention

[0009] To address the aforementioned problems, this invention provides a compound treatment solution for Daphne odora seeds with Epimedium polysaccharide as the main active ingredient and its application, in order to solve the problems of low germination rate, high mold rate, and poor seedling quality in existing Daphne odora seed treatment technologies.

[0010] To achieve the above objectives, the technical solution adopted by the present invention is as follows: A compound treatment solution for Daphne odora seeds with Epimedium polysaccharide as the main active ingredient, wherein the Daphne odora seed compound treatment solution comprises gibberellin 100-300 mg / L, Epimedium polysaccharide 0.1-100 mg / L, 6-benzylaminopurine 15-35 mg / L, oligosaccharide 50-150 mg / L, benzothiadiazole 1-30 mg / L and fungicide 20-60 mg / L.

[0011] Furthermore, the compound treatment solution for Daphne odora seeds also includes 0-1 mL / L of surfactant.

[0012] Furthermore, the oligosaccharide is chitosan oligosaccharide or chitin oligosaccharide.

[0013] Furthermore, the oligosaccharides have a degree of polymerization of 2 to 10 and a water solubility of greater than 98%.

[0014] Furthermore, the fungicide is thiamethoxam, fludioxonil, or carbendazim.

[0015] Furthermore, the preparation method of the Daphne odora seed compound treatment solution includes the following steps: After dissolving gibberellin in ethanol, GA3 stock solution was obtained; Dissolve 6-benzylaminopurine in NaOH aqueous solution to obtain 6-BA mother liquor; Dissolve the oligosaccharide in water to obtain the chitosan oligosaccharide mother liquor; Dissolve benzothiadiazole in ethanol to obtain BTH mother liquor; Take the mother liquor and Epimedium polysaccharide in sequence, add them to water, mix well, then add bactericide and surfactant, mix well, and you will get the Daphne odora seed compound treatment solution.

[0016] An application of a compound treatment solution for Daphne odora seeds with Epimedium polysaccharide as the main active ingredient, wherein the application involves treating Daphne odora seeds or Daphne tangutica seeds with the above-mentioned compound treatment solution.

[0017] A method for treating Daphne odora seeds, wherein the method involves soaking Daphne odora seeds in the above-mentioned Daphne odora seed compound treatment solution to improve the germination rate and germination potential of Daphne odora seeds, increase the height and root length of Daphne odora seedlings, and reduce the mold rate.

[0018] Furthermore, the processing method includes the following steps: Seed disinfection: Soak seeds in a 1% potassium permanganate solution for 1-4 hours, then rinse with sterile water; Seed soaking treatment: Soak the seeds of Daphne odora in the compound treatment solution described above for 15-20 hours in a dark environment at 20-27°C; Low-temperature stratification: Soaked seeds are mixed with moist, sterilized river sand at a weight ratio of 1:2.5~3.5 and then stratified at low temperature. Sowing and germination: After stratification, the seeds are sown in a flat paper bed or seedling substrate and cultured at 20-25℃ with a photoperiod of 16h / 8h.

[0019] Furthermore, during the low-temperature lamination process, the lamination time is 50-90 days and the temperature is 3-5℃, with the preferred time being 70 days and the temperature being 4℃.

[0020] The beneficial effects of the compound treatment solution of Daphne odora seeds with Epimedium polysaccharide as the main active ingredient and its application are as follows: There is no prior art documenting the use of Epimedium polysaccharide for seed treatment in crops to improve seed germination efficiency. This invention develops a composite treatment solution with Epimedium polysaccharide as the main active ingredient, and uses it to treat Daphne odora seeds. The treated Daphne odora seeds show significantly improved germination and seedling rates, and robust seedling growth. The mechanism of action may lie in the fact that the "system activation" and "cell metabolism promotion" properties of Epimedium polysaccharide can directly target the systemic problem of seed dormancy. By promoting DNA synthesis and cell proliferation, it directly acts on the embryo, promoting its development and breaking physiological dormancy. Its antiviral / antimicrobial properties can correspondingly activate the seed's systemic resistance, improving resistance to fungal infections during stratification and helping to resist microbial infection on the seed surface. This provides an innovative solution to the problems of seed dormancy and artificial propagation in Daphne odora. This invention provides a compound treatment solution for Daphne odora seeds with Epimedium polysaccharide as the main active ingredient and its application. By soaking Daphne odora seeds in the compound treatment solution and then subjecting the soaked seeds to low-temperature stratification, the synergistic dormancy-breaking effect is significant, completely breaking the physiological dormancy of the seeds, significantly improving the germination rate, resulting in robust seedlings, and effectively inhibiting mold during the stratification period. The process is standardized and widely applicable. The treatment solution can also be applied to the treatment of Daphne tangutica seeds. Experimental results of this invention show that soaking Daphne odora seeds in the compound treatment solution of this invention, combined with low-temperature stratification, can improve the germination rate, germination potential, seedling height, and seedling root length of Daphne odora seeds, while reducing the mold rate and shortening the dormancy-breaking period. Therefore, the compound treatment solution of this invention can improve the germination characteristics of Daphne odora seeds, enhance seed emergence and seedling quality, and improve resistance to mold. The composite treatment liquid and treatment method of the present invention can significantly improve seed germination rate and germination potential, effectively inhibit mold growth during stratification, enhance seedling growth potential, and provide a standardized and repeatable operating procedure, which is suitable for large-scale production. Detailed Implementation

[0021] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Many specific details are set forth in the following description to provide a thorough understanding of the present invention. However, the present invention may also be implemented in other ways different from those described herein. Those skilled in the art can make similar extensions without departing from the spirit of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0022] Materials: Plump yellow daphne seeds collected in Tianzhu, Gansu in 2023; Epimedium polysaccharide purchased from Shanghai Yuanye Biotechnology Co., Ltd. (specification: 50%, S24632); Chitosan oligosaccharide purchased from Shandong Weikang Biomedical Technology Co., Ltd. (average molecular weight 1500 Da, degree of polymerization 2~10, degree of deacetylation ≥90%).

[0023] Example 1: A compound treatment solution for Daphne odora seeds with Epimedium polysaccharide as the main active ingredient and its application. This embodiment describes a compound treatment solution for Daphne odora seeds with Epimedium polysaccharide as the main active ingredient and its application. The preparation method of the compound treatment solution is as follows: Take an appropriate amount of gibberellin (GA3), dissolve it in ethanol and make up the volume to obtain GA3 stock solution (10g / L). Take an appropriate amount of 6-benzylaminopurine (6-BA), add a small amount of 0.5 mol / L NaOH aqueous solution to dissolve it, and then make up the volume to obtain 6-BA stock solution (1 g / L). Chitosan oligosaccharide with a relative molecular weight of 1500 and a degree of polymerization of 2-10 was directly dissolved in deionized water to obtain chitosan oligosaccharide mother liquor (10g / L). Dissolve benzothiadiazole (BTH) in ethanol to obtain BTH stock solution (1 g / L). Take appropriate amounts of each mother liquor and Epimedium polysaccharide and add them to deionized water. Stir well, then add appropriate amounts of bactericide (thiabendazole) and surfactant Tween-20. Add deionized water to a final volume of 1L and mix well to obtain the composite treatment solution of Daphne odora seeds with Epimedium polysaccharide as the main active ingredient. The composite treatment solution contains 100mg / L Epimedium polysaccharide, 150mg / L GA3, 30mg / L 6-BA, 100mg / L chitosan oligosaccharide, 15mg / L benzothiadiazole, 40mg / L thiabendazole, and 0.5ml / L Tween-20.

[0024] A method for treating *Daphne odora* seeds with a compound treatment solution containing *Epimedium* polysaccharide as the main active ingredient includes the following steps: Seed disinfection: Soak Daphne odora seeds in a 1% potassium permanganate solution for 2 hours, then rinse with sterile water; Seed soaking treatment: Soak the seeds in a compound treatment solution in a dark environment at 25°C for 16 hours; Low-temperature stratification: After soaking, the seeds are mixed with moist sterilized river sand at a weight ratio of 1:3, and then stratified at 4℃ for 70 days. Sowing and germination: After stratification, the seeds are sown in a flat paper bed or seedling substrate (in this example, they are sown in a flat paper bed) and cultured at 20-25℃ under a light cycle of 16h / 8h (light / dark).

[0025] Example 2: Comparison of germination effects between a compound treatment solution for Daphne odora seeds with Epimedium polysaccharide as the main active ingredient and conventional treatment methods. Experimental group: The seeds of Daphne odora were treated with the composite treatment solution of Example 1 according to the treatment method in Example 1, and were designated as experimental group T1.

[0026] Conventional stratification control group: The seeds of Daphne odora were treated according to the treatment method in Example 1. The steps and process parameters were basically the same as those in Example 1. The only difference was that water was used instead of the compound treatment solution for soaking. This group was used as the conventional stratification control group CK1.

[0027] Single hormone control group: Take an appropriate amount of the GA3 mother liquor prepared in Example 1 and add it to the deionized water. Stir well and make up the volume to obtain a GA3 solution with a concentration of 200 mg / L. Then treat the seeds of Daphne odora according to the treatment method in Example 1. The steps and process parameters are basically the same as those in Example 1. The only difference is that the GA3 solution with a concentration of 200 mg / L is used instead of the compound treatment solution for soaking treatment. This group is used as the single hormone control group CK2.

[0028] Subsequently, the germination rate, germination potential, and stratification period mold rate of *Daphne odora* seeds in each group were observed and recorded. Growth indicators (plant height and root length) of *Daphne odora* seedlings were measured 30 days after sowing. The calculation methods for germination rate, germination potential, and stratification period mold rate are as follows: Germination rate (%) = (Number of normally germinated seeds / Number of tested seeds) × 100% Germination potential (%) = (Number of germinated seeds on day 10 / Number of seeds tested) × 100% Seedling growth indicators: plant height and root length (measured 30 days after sowing). Mold contamination rate (%) = (Number of moldy seeds during stratification / Number of tested seeds) × 100% The results of the comparison of germination rate and germination potential for each group are shown in the table below.

[0029] Table 1 Effects of different treatments on germination rate and germination potential of Daphne odora seeds

[0030] Note: Different letters in the same column indicate significant differences at the P < 0.05 level.

[0031] As shown in Table 1, the germination rate and germination potential of the experimental group T1 of this invention were significantly higher than those of the control groups (P<0.05). Among them, the germination rate of the experimental group T1 was significantly higher than that of the conventional stratification control group CK1 and the single hormone control group CK2, proving that the composite treatment solution of this invention can effectively break seed dormancy.

[0032] The growth status of seedlings in each group is shown in the table below.

[0033] Table 2. Effects of different treatments on the growth of Daphne odora seedlings (30 days after sowing)

[0034] As shown in Table 2, the seedlings of experimental group T1 of this invention grew vigorously, with significantly better plant height and root length than those of the control groups. While treatment of seeds with the single hormone control group CK2 followed by low-temperature stratification resulted in a higher germination rate for Daphne odora seeds, the emergence rate was poor, which is consistent with the findings in the literature.

[0035] The resistance to mold growth in each group is shown in the table below.

[0036] Table 3 Comparison of seed mold rates during stratification

[0037] As can be seen from Table 3, during the lamination process, no mold growth was observed in the experimental group T1 of this invention, while the mold growth rate was relatively high in each control group.

[0038] Example 3: Adaptability Study of Different Lamination Times Experimental group: The composite treatment solution of Example 1 was used to treat the seeds of Daphne odora according to the treatment method in Example 1. The steps and process parameters were basically the same as those in Example 1, except that the stratification time was 50 days, 70 days and 90 days respectively. This group was used as the experimental group.

[0039] Optimal hormone combination control group: Take appropriate amounts of GA3 mother liquor and 6-BA mother liquor prepared in Example 1 and add them to deionized water. Stir well and make up to a final volume to obtain a mixed solution containing 150 mg / L GA3 and 30 mg / L 6-BA. Then treat the seeds of Daphne odora according to the treatment method in Example 1. The steps and process parameters are basically the same as those in Example 1. The only difference is that the mixed solution containing 150 mg / L GA3 and 30 mg / L 6-BA is used instead of the composite treatment solution for soaking. After soaking, the seeds are mixed with moist sterilized river sand at a weight ratio of 1:3 and then stratified at 4°C for 50 days, 70 days and 90 days respectively. This group is used as the optimal hormone combination control group CK3.

[0040] The effects of each group on the germination rate of Daphne odora seeds were then observed, and the shortest effective stratification time was investigated. The results are shown in the table below.

[0041] Table 4 Effect of stratification time on germination rate (%) of Daphne odora seeds treated with the solution of this invention

[0042] As shown in Table 4, using the composite treatment solution of the present invention, a high germination rate of 76.3% can be achieved in just 50 days of stratification, while the germination rate of the traditional hormone combination (i.e., the optimal hormone combination control group) is only 36.3% in the same period. This indicates that the composite treatment solution of the present invention can significantly shorten the dormancy period and improve seedling efficiency, which is crucial for large-scale production.

[0043] Example 4: Verification of seedling quality and field emergence rate Seeds from each group in Example 2 and the control group CK3 (optimal hormone combination) in Example 3, after 70 days of stratification, were sown in seedling trays and cultivated in a greenhouse. Germination rates were recorded for each group, and seedling height and root length were measured 40 days after seedling emergence to assess seedling vigor. The results are shown in the table below.

[0044] Table 5 Effects of different treatments on seedling emergence rate and growth of Daphne odora seedlings in the field.

[0045] As can be seen from Table 5, the seeds treated with the composite treatment solution of the present invention not only have a high germination rate, but also can cultivate high-quality seedlings with well-developed root systems and robust plants. The field emergence rate (83.5%) is significantly higher than that of other treatment groups, which proves its excellent seedling quality and seedling growth ability.

[0046] Example 5: Verification of the effectiveness of the treatment fluid replacement scheme To demonstrate the flexibility of the formulation of this invention, alternative solutions were examined, as follows: Alternative Group: The composite treatment solution was prepared according to the preparation method of the composite treatment solution in Example 1. The steps and process parameters were basically the same as those in Example 1. The only difference was that 100 mg / L carbendazim was used instead of 40 mg / L thiabendazim, and 100 mg / L chitosan oligosaccharide was used instead of 100 mg / L chitosan oligosaccharide. The composite treatment solution was used to treat Daphne odora seeds in the same way as in Example 1. This group was used as Alternative Group T.

[0047] The germination rate, germination potential, and mold rate during the stratification period of Daphne odora seeds in the substitution group T, the experimental group T1 in Example 2, and the conventional stratification control group CK1 were compared respectively. The results are shown in the table below.

[0048] Table 6 Impact of Key Indicators

[0049] As shown in Table 6, there was no significant difference in key indicators between the substitution group T and the experimental group T1, indicating that carbendazim can replace thiabendazole as a fungicide in the preparation of the compound treatment solution, and chitosan oligosaccharide can replace chitosan oligosaccharide in the preparation of the compound treatment solution.

[0050] Example 6: Comparison Experiment of Single-Component Effects To further demonstrate the synergistic effect among the components in the composite treatment solution of this invention, especially the necessity of Epimedium polysaccharide in the specific system of this invention and its synergistic effect with other components, rather than its single effect, the following single-component control experiment was designed, as follows: Positive control group: The seeds of Daphne odora were treated with the compound treatment solution of Example 1 according to the treatment method in Example 1, and were used as experimental group T1'.

[0051] Single Epimedium Polysaccharide Group: An appropriate amount of Epimedium polysaccharide was added to deionized water, stirred evenly, and diluted to a final volume to obtain an Epimedium polysaccharide solution with a concentration of 100 mg / L; then, the seeds of Daphne odora were treated according to the treatment method in Example 1. The steps and process parameters were basically the same as those in Example 1, except that the Epimedium polysaccharide solution was used instead of the composite treatment solution for soaking treatment. This group was designated as the single Epimedium polysaccharide group S1.

[0052] Gibberellin group: Take an appropriate amount of the GA3 mother liquor prepared in Example 1 and add it to the deionizer. Stir well and make up the volume to obtain a GA3 solution with a concentration of 200 mg / L. Then treat the seeds of Daphne odora according to the treatment method in Example 1. The steps and process parameters are basically the same as those in Example 1. The only difference is that the GA3 solution with a concentration of 200 mg / L is used instead of the composite treatment solution for soaking treatment. This group is used as the gibberellin group S2.

[0053] Single 6-benzylaminopurine group: Take an appropriate amount of the 6-BA mother liquor prepared in Example 1 and add it to the deionized water. Stir well and make up the volume to obtain a 6-BA solution with a concentration of 25 mg / L. Then treat the seeds of Daphne odora according to the treatment method in Example 1. The steps and process parameters are basically the same as those in Example 1. The only difference is that the 6-BA solution with a concentration of 25 mg / L is used instead of the composite treatment solution for soaking treatment. This group is used as the single 6-benzylaminopurine group S3.

[0054] Blank control group: The seeds of Daphne odora were treated according to the treatment method in Example 1. The steps and process parameters were basically the same as those in Example 1. The only difference was that deionized water was used instead of the composite treatment solution for soaking. This group was used as the blank control group S4.

[0055] The germination rate, germination potential, stratification period mold rate, and seedling height and root length after 30 days of seedling growth were then observed and recorded. The results are shown in the table below.

[0056] Table 7 Comparison of the effects of single-component treatment and composite treatment solutions

[0057] Note: Different letters in the same column indicate significant differences at the P < 0.05 level.

[0058] As shown in Table 7, although some indicators of the treatment with Epimedium polysaccharide (S1) or other single hormones (S2, S3) were better than those of the blank control (S4), they were all significantly lower than those of the compound treatment solution of the present invention (T1'). In particular, the effect of using Epimedium polysaccharide alone was far from achieving the significant improvement in the seed treatment level of Daphne odora in the compound treatment solution of the present invention.

[0059] The composite treatment solution of the present invention exhibits comprehensive and significant advantages in germination rate, germination potential, resistance to mold, and seedling vigor. Its overall effect is far beyond the simple sum of the effects of each individual component.

[0060] Example 7: Applications of other Daphne species The composite treatment solution prepared in Example 1 of this invention was applied to Tangut Daphne seeds according to the treatment method of Example 1. The germination rate of Tangut Daphne seeds increased from 32% in the control group treated with water to 79%, and the mold rate decreased from 12% to 1.5%, proving that the composite treatment solution of this invention has cross-species applicability.

[0061] The above experimental results show that the composite treatment solution and treatment method of the present invention can improve the vigor of Daphne seeds (including Daphne tangutica seeds, Daphne tangutica seeds, etc.) and the quality of seedlings, and effectively inhibit mold growth during the stratification period.

[0062] Examples 8-11: Compound treatment solution of Daphne odora seeds with Epimedium polysaccharide as the main active ingredient and its application Examples 8-11 are a compound treatment solution for Daphne odora seeds with Epimedium polysaccharide as the main active ingredient and its application. The steps are basically the same as those in Example 1, except for the different process parameters. See Table 8 for details. Table 8. Summary of process parameters in Examples 8-11

[0063] The process parameters and steps for the other parts of Examples 8-11 are the same as those for Example 1.

[0064] Obviously, the described embodiments are only some, not all, of the embodiments of the present invention. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without inventive effort are within the scope of protection of the present invention.

Claims

1. A compound treatment liquid for Epimedium seed, with icariin polysaccharide as the main effective component, characterized in that, The seed composite treatment liquid of the Daphne giraldii seed comprises gibberellin 100-300 mg / L, icariin 0.1-100 mg / L, 6-benzylaminopurine 15-35 mg / L, oligosaccharide 50-150 mg / L, benzothiadiazole 1-30 mg / L and fungicide 20-60 mg / L.

2. The Epimedium polysaccharide as the main effective component of the seed composite treatment liquid of the yellow Swiss, according to claim 1, characterized in that, The seed composite treatment liquid of the Daphne giraldii seed further comprises surfactant 0-1 mL / L.

3. The Epimedium polysaccharide as the main effective component of the seed composite treatment liquid of the Epimedium according to claim 1 or 2, characterized in that, The oligosaccharide is chitooligosaccharide or chitin oligosaccharide.

4. The Epimedium polysaccharide as the main effective component of the seed composite treatment liquid of the Epimedium according to claim 1 or 2, characterized in that, The oligosaccharide has a degree of polymerization of 2-10.

5. The Epimedium polysaccharide as the main effective component of the seed complex treatment liquid of the Epimedium according to claim 1 or 2, characterized in that, The fungicide is thiabendazole, fludioxonil or carbendazim.

6. The Epimedium polysaccharide as the main effective component of the seed complex treatment liquid of the Epimedium according to claim 1 or 2, characterized in that, The preparation method of the seed composite treatment liquid of the Daphne giraldii seed comprises the following steps: gibberellin is taken and dissolved in ethanol to obtain a GA3 mother liquor; 6-benzylaminopurine is taken and dissolved in NaOH aqueous solution to obtain a 6-BA mother liquor; oligosaccharide is taken and dissolved in water to obtain a chitooligosaccharide mother liquor; benzothiadiazole is taken and dissolved in ethanol to obtain a BTH mother liquor; the mother liquors and icariin are taken in water in sequence, mixed, and then fungicide and surfactant are added and mixed to obtain the seed composite treatment liquid of the Daphne giraldii seed.

7. The use of a compound treatment liquid of Epimedium seed with icariin as the main effective component, characterized in that, The application is to treat Daphne giraldii seed or Daphne tangutorum seed with the seed composite treatment liquid of the Daphne giraldii seed according to any one of claims 1-6.

8. A method of treating seeds of T. suaveolens, characterized in that, The treatment method is to soak Daphne giraldii seed with the seed composite treatment liquid of the Daphne giraldii seed according to any one of claims 1-6 to improve the germination rate and germination energy of the Daphne giraldii seed, improve the seedling height and root length of the Daphne giraldii seedling and reduce the moldy rate.

9. The method of treating the seeds of T. Aurea according to claim 8, characterized in that, The treatment method comprises the following steps: seed disinfection: soaking with potassium permanganate aqueous solution and washing with sterile water; seed soaking treatment: soaking with the seed composite treatment liquid of the Daphne giraldii seed in a dark environment; low-temperature stratification: mixing the soaked seed with river sand and stratifying at low temperature; seed sowing and germination: sowing the stratified seed on a paper bed or in a seedling substrate.

10. The method of treating the seeds of T. Aurea according to claim 9, characterized in that, During the low-temperature stratification, the stratification time is 50-90 days and the temperature is 3-5℃.

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