Application of antipyrine in promoting seed germination under drought condition

Abstract

The invention discloses an application of antipyrine in promoting seed germination under a drought condition. The lower limit of concentration of antipyrine for promoting seed germination under the drought condition is 12.5 [mu] mol.L <-1 >. According to the method, antipyrine is used for soaking the seeds under the drought condition, the stress resistance of the seeds can be enhanced, antipyrine with a certain concentration can enhance the activity of related enzymes of the seeds and synthesis of protein, the germination rate of the seeds under the drought condition can be increased, and the germination rate of the seeds under the drought condition can be increased. Meanwhile, the germination index and the vitality index of the seeds under drought stress can be improved.

Description

Technical Field

[0001] This invention relates to seed germination and emergence, and more specifically, to the application of antipyrine in promoting seed germination under drought conditions. Background Technology

[0002] In agricultural production, crop seeds often have long germination times and low germination rates, resulting in uneven crop emergence. Therefore, achieving a high emergence rate after crop sowing is a pressing technical problem that needs to be solved.

[0003] In recent years, drought and water scarcity caused by global warming, the intensified greenhouse effect, and rising ambient temperatures have become pressing problems that urgently need to be addressed. Drought is one of the major limiting factors restricting global crop production. Statistics show that approximately 33% of the world's arable land is suffering from periodic or unpredictable droughts, particularly in arid or semi-arid regions affected by global climate change. Seed germination is a necessary stage of plant growth, and drought stress is one of the most severe abiotic stresses limiting seed germination.

[0004] In recent years, affected by global climate change, the drought-affected area in most parts of my country has been increasing, with a significant increase in the North China Plain and Xinjiang region. Since the North China Plain and Xinjiang are major production areas for grain crops and medicinal herbs in my country, improving the drought resistance of plants and enhancing their survival ability under extreme climatic conditions is crucial for maintaining their growth, development, yield, and quality. Summary of the Invention

[0005] To address the problem of drought stress limiting seed germination, this invention provides the application of antipyrine in promoting seed germination under drought conditions. By applying this drug, the adverse effects of drought stress on seed growth can be effectively alleviated, and the drought resistance of crops can be improved, thus contributing to the sustainable development of agriculture.

[0006] To achieve the above objectives, the present invention provides an application of antipyrine in promoting seed germination under drought conditions, thereby improving crop survival rate and yield.

[0007] Specifically, the lower limit of concentration for antipyrine to promote seed germination is 12.5 μmol·L⁻¹. -1 .

[0008] Preferably, the seed is a seed from the Asteraceae family.

[0009] Specifically, the seeds are chicory seeds, artemisia seeds, and wild horse-tail seeds.

[0010] Through the above technical solution, the present invention achieves the following beneficial effects: This invention provides a method for promoting seed germination and emergence under drought conditions using antipyrine. Soaking the seeds enhances their stress resistance. A certain concentration of antipyrine can enhance the activity of seed-related enzymes and the synthesis of proteins, which can not only improve the germination rate of seeds under drought conditions, but also improve the germination index and vigor index of seeds under drought stress. Attached Figure Description

[0011] Figure 1 The germination rate of chicory seeds treated with different concentrations of antipyrine in Example 1 of this invention under drought stress; statistical analysis of the data was performed using a t-test. # The asterisk (*) represents the reference material used as a control, and the ** represents p < 0.01. Figure 2 It is 50 μmol·L in Example 1 -1 Effects of antipyrine treatment on seed germination and growth of chicory under drought stress, where (A) represents different groups of chicory; (B) represents germination rate; (C) represents germination potential; (D) represents germination index; and (E) represents vigor index. Figure 3 The SOD activity of chicory seeds treated with different concentrations of antipyrine in Example 1 under drought stress; Figure 4 This refers to the germination rate of Artemisia annua seeds treated with different concentrations of antipyrine in Example 2 of this invention under drought stress and normal conditions; Figure 5 This refers to the germination rate of *Pterocarya esculenta* seeds treated with different concentrations of antipyrine in Example 3 of this invention under drought stress and normal conditions. Detailed Implementation

[0012] The specific embodiments of the present invention will be described in detail below with reference to examples. It should be understood that the specific embodiments described herein are for illustration and explanation only and are not intended to limit the present invention.

[0013] Example 1: Antipyrine promotes the germination of chicory seeds Step 1: Seed selection. Randomly select plump, mold-free, and vigorous chicory seeds ready for sowing. Then, wrap the selected seeds in a single layer using a sterile microporous mesh. Step 2: Grouping. The control group consisted of chicory seeds treated with deionized water, the model group consisted of chicory seeds treated with 10% polyethylene glycol-6000 (simulating drought stress), and the experimental groups consisted of different concentrations (12.5, 25, 50, 100, 200 μmol·L⁻¹). -1 Antipyrine and 10% polyethylene glycol-6000 were co-treated with chicory seeds; Step 3: Soaking. Soak the wrapped chicory seeds in 1% H2O2 for 10 minutes for disinfection. Finally, rinse them 5 times with deionized water. After washing, place them in 50mL centrifuge tubes after the grouping in Step 2 and soak them in the corresponding reserve solution for 24 hours before use. Step 4: Cultivation. Take out the plump and uniform chicory seeds that have been soaked overnight from Step 2, and blot off the surface moisture with filter paper. Then, evenly place the chicory seeds into a petri dish (100mm×20mm) lined with a single layer of sterile filter paper and add 3mL of treatment solutions of different concentrations, with 40 seeds in each petri dish. Step 5: Statistics. Germination was carried out in an artificial climate incubator with a temperature of 25℃, a light intensity of 12000 Lux, full light, and a humidity of 70%. Every afternoon, the corresponding solution was added to each group of petri dishes by weighing until a constant mass was reached to maintain a constant solution concentration. The number of germinated seeds was counted daily during the experiment, with cotyledon expansion as the standard. At the end of the experiment, the germination rate and germination potential were counted.

[0014] Figure 1 The germination rate of chicory seeds treated with different concentrations of antipyrine under drought conditions is shown in the figure. The figure reveals that each concentration of antipyrine significantly increased the germination rate of chicory seeds under drought conditions, with the highest concentration being 50 μmol·L⁻¹. -1 At this time, the germination rate of chicory seeds under drought conditions is best improved.

[0015] Figure 2 50 μmol·L -1 Effects of antipyrine treatment on seed germination and growth of chicory under drought stress. As shown in the figure, the experimental group treated with 50 μmol·L⁻¹ antipyrine showed the best germination and growth. -1 Compared with the model group treated with 10% polyethylene glycol-6000, the antipyrine treatment significantly improved the germination rate, germination potential, germination index and seedling vigor index of chicory seeds, increasing by 80.0%, 83.3%, 79.9% and 88.8% respectively, thereby enhancing the drought resistance of chicory seeds.

[0016] Figure 3 The study investigated the SOD activity of chicory seeds treated with different concentrations of antipyrine under drought conditions. As shown in the figure, all antipyrine concentrations significantly increased the SOD activity of chicory seeds under drought conditions, with the highest concentration being 50 μmol·L⁻¹. -1 The optimal time to improve the SOD activity of chicory seeds under drought conditions is during this period.

[0017] Example 2: Antipyrine promotes Artemisia annua seed germination Step 1: Seed selection. Randomly select plump, mold-free, and vigorous Artemisia annua seeds ready for sowing. Then, wrap the selected seeds in a single layer using a sterile microporous mesh. Step 2: Grouping. The control group received Artemisia annua seeds treated with deionized water, the model group received Artemisia annua seeds treated with 10% polyethylene glycol-6000 (simulating drought stress), and the experimental groups received different concentrations (12.5, 25, 50, 100, 200 μmol·L⁻¹). -1 Antipyrine and 10% polyethylene glycol-6000 were used to co-treat Artemisia annua seeds; Step 3: Soaking. Soak the Artemisia annua seeds that have been wrapped and treated in Step 1 in 1% H2O2 for 10 minutes for disinfection. Finally, rinse them 5 times with deionized water. After washing, place them in 50mL centrifuge tubes that were grouped in Step 2 and soak them in the corresponding reserve solution for 24 hours before use. Step 4: Cultivation. Take out the plump and uniform Artemisia annua seeds that have been soaked overnight from Step 2, and use filter paper to absorb the surface moisture. Then, evenly place the Artemisia annua seeds into a culture dish (100mm×20mm) lined with a single layer of sterile filter paper and add 3mL of treatment solutions of different concentrations, with 40 seeds in each culture dish. Step 5: Statistics. Germination was carried out in an artificial climate incubator with a temperature of 25℃, a light intensity of 12000 Lux, full light, and a humidity of 70%. Every afternoon, the corresponding solution was added to each group of petri dishes by weighing until a constant mass was reached to maintain a constant solution concentration. The number of germinated seeds was counted daily during the experiment, with cotyledon expansion as the standard. At the end of the experiment, the germination rate and germination potential were counted.

[0018] Figure 4 The figure shows the germination rate of Artemisia annua seeds treated with different concentrations of antipyrine under drought conditions. As can be seen from the figure, the germination rate of Artemisia annua seeds under drought conditions first increases and then decreases with the increase of antipyrine concentration.

[0019] Example 3: Antipyrine promotes seed germination in Przewalski's horses. Step 1: Seed selection. Randomly select plump, mold-free, and vigorous wild horse seeds ready for sowing. Then, wrap the selected seeds in a single layer using a sterile microporous mesh. Step 2: Grouping. The control group received deionized water-treated *Pterocarya stenoptera* seeds, the model group received 10% polyethylene glycol-6000-treated *Pterocarya stenoptera* seeds (simulating drought stress), and the experimental groups received different concentrations (12.5, 25, 50, 100, 200 μmol·L⁻¹). -1 Antipyrine and 10% polyethylene glycol-6000 were used to co-treat the seeds of Wild Horse Chaser; Step 3: Soaking. Soak the wild horse seeds that have been wrapped and treated in Step 1 in 1% H2O2 for 10 minutes for disinfection. Finally, rinse them 5 times with deionized water. After washing, place them in 50mL centrifuge tubes that were grouped in Step 2 and soak them in the corresponding reserve solution for 24 hours before use. Step 4: Cultivation. Take out the plump and uniform wild horse seeds that have been soaked overnight from Step 2, and use filter paper to absorb the surface moisture. Then, evenly place the wild horse seeds into a culture dish (100mm×20mm) lined with a single layer of sterile filter paper and add 3mL of treatment solutions of different concentrations, with 40 seeds in each culture dish. Step 5: Statistics. Germination was carried out in an artificial climate incubator with a temperature of 25℃, a light intensity of 12000 Lux, full light, and a humidity of 70%. Every afternoon, the corresponding solution was added to each group of petri dishes by weighing until a constant mass was reached to maintain a constant solution concentration. The number of germinated seeds was counted daily during the experiment, with cotyledon expansion as the standard. At the end of the experiment, the germination rate and germination potential were counted.

[0020] Figure 5 The figure shows the germination rate of *Pterocarya stenoptera* seeds treated with different concentrations of antipyrine under drought conditions. As can be seen from the figure, the germination rate of *Pterocarya stenoptera* seeds under drought conditions first increases and then decreases with increasing antipyrine concentration.

[0021] The preferred embodiments of the present invention have been described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the scope of the technical concept of the present invention, various simple modifications can be made to the technical solution of the present invention, and these simple modifications all fall within the protection scope of the present invention.

[0022] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not describe the various possible combinations separately.

[0023] Furthermore, various different embodiments of the present invention can be combined in any way, as long as they do not violate the spirit of the present invention, they should also be regarded as the content disclosed by the present invention.

Claims

1. The use of antipyrine in the promotion of seed germination under drought conditions.

2. Use according to claim 1, characterized in that, The lower limit of the concentration of the antipyrine promoting seed germination is 12.5 μmol·L -1 .

3. Use according to claim 1, characterized in that, The seeds are seeds of the Asteraceae family.

4. Use according to claim 3, characterized in that, The seeds are seeds of Cichorium intybus, Artemisia annua, or Eupatorium odoratum.

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