Functional pet food for regulating intestinal health and preparation method thereof

Abstract

The invention belongs to the field of pet food processing, and relates to a functional pet food for regulating intestinal health and a preparation method thereof. The functional pet food can increase MTL content in dog serum in a physiological range, reduce DAO activity and D-lactic acid and LPS content in dog serum, improve intestinal mucosa barrier function, and significantly improve dog intestinal health. The functional pet food can shorten gastrointestinal transmission time and effectively improve constipation. The functional pet food disclosed by the invention has a good effect in the aspects of treating diarrhea and constipation, and has a two-way regulation effect in regulating intestinal flora imbalance. The components of the raw materials have a synergistic effect, the nutrition is balanced, the preparation method is simple, the palatability is optimally designed, and the metabolic characteristic is free of burden, so that pets can safely take the feed for a long time, a convenient and efficient preventive health management scheme is provided for family pet raising, and the feed has a relatively good commercial application prospect.

Description

Technical Field

[0001] This invention belongs to the field of pet food processing and relates to a functional pet food for regulating intestinal health and its preparation method. Background Technology

[0002] With the development of human society and economy, the number of elderly people living alone in cities is increasing, and the number of pet owners is also growing. The bond between people and their pets is deepening, and many pets are being treated as "family members." However, due to the special nature of pet ownership, the nutrition of pet food differs significantly from that of traditional livestock and poultry. As companions and emotional anchors for people, the health status and age of pets are receiving considerable attention.

[0003] The gut is a vital organ for digestion and absorption, and also the largest immune organ; its health is closely related to a pet's overall health. As carnivores, dogs and cats are highly susceptible to gastrointestinal diseases such as vomiting, diarrhea, and constipation. Antibiotics play a crucial role in treating these diseases; however, excessive antibiotic use can lead to bacterial resistance, disrupt the gut microbiota balance, and seriously endanger a pet's health. Changes in the gut microbiota of dogs and cats also affect normal bodily functions. When abnormalities occur, the cause is often the invasion of harmful bacteria into the gut, as well as an imbalance in the gut microbiota, which affects many important metabolic processes, such as bile acid metabolism and fermentation, and amino acid absorption.

[0004] Constipation is a series of clinical symptoms caused by one or more factors that slow down intestinal peristalsis and weaken intestinal contraction, resulting in sluggish movement or retention of intestinal contents in the large intestine lumen. Large amounts of water are absorbed, causing the stool to become dry and hard, leading to obstruction, delay, or even cessation of defecation. It can be divided into organic constipation and functional constipation. The causes of constipation are multifaceted, broadly categorized into feeding and management factors and disease factors. For example, hard food or food containing foreign objects such as sand or string is considered improper feeding and management. Constipation caused by prostatitis, common in older dogs, is a disease factor. In prostatitis, abdominal palpation or digital rectal examination may reveal an enlarged, painful, hard, or fluctuating prostate. If fluctuation is felt upon palpation, it may indicate prostatic abscess formation. Primary causes of constipation also include metabolic and endocrine disorders, intestinal obstruction, tumors, or rectal stenosis. If constipation is not detected promptly, it can lead to serious consequences such as enteritis, intestinal perforation, or bleeding. Chronic constipation, also known as idiopathic constipation or functional constipation, has a complex etiology. It can be caused by impaired colonic transit capacity (motor incoordination) or anal sphincter dysfunction. Furthermore, numerous digestive tract diseases, medications, and abnormalities in the nervous, endocrine, or metabolic systems can also lead to chronic constipation. Colorectal surgeons categorize chronic constipation requiring special clinical management into slow transit constipation and outlet obstruction constipation. Outlet obstruction constipation includes rectocele, rectal mucosal prolapse, puborectalis muscle syndrome, and pelvic floor spasm syndrome.

[0005] Slow transit constipation, caused by weakened colonic transit function and slow passage of intestinal contents, is the most common type of constipation in clinical practice. Its etiology is unclear, symptoms are persistent, and treatment is difficult. The main characteristics are reduced bowel movement frequency, loss of urge to defecate, and difficulty in defecation. Many patients have a history of long-term laxative use. Currently, symptomatic treatment with medication is common, but dependence is easily developed, and constipation recurs after discontinuation. Subtotal or total colectomy is a reliable long-term treatment for slow transit constipation, but complications such as intestinal adhesions and increased bowel movement frequency are unavoidable, and further surgery may be necessary due to adhesive intestinal obstruction.

[0006] Diarrhea refers to an animal's defecation frequency significantly exceeding its usual frequency, with loose stools that change in form, color, and odor. The stool may contain pus, blood, mucus, undigested food, fat, or become yellowish-watery or greenish-paste with a sour, foul odor. Symptoms may include abdominal pain, tenesmus, and incontinence during defecation. In the classic Chinese medicine text *Neijing*, diarrhea is called "xie," during the Han and Tang dynasties it was called "xiali," and after the Song dynasty it was called "xiexie," commonly known as "laxi." In veterinary clinical practice, diarrhea can be classified as acute or chronic based on the speed of onset. Acute diarrhea typically has a rapid onset and a course of 2-3 weeks; chronic diarrhea lasts for more than 2 months or is recurrent diarrhea with intervals of 2-4 weeks. Based on the frequency and form of the stool, it can be classified as diarrhea or dysentery. Currently, commonly used medications for treating pet diarrhea are antibiotics or chemical drugs such as loperamide, sulfasalazine, and loperamide. However, these drugs are not ideal for treatment, and overuse of antibiotics can lead to severe drug resistance in pets, making a complete cure for diarrhea impossible.

[0007] The types and levels of nutrients in pet food are closely related to the gut health of pets. Therefore, how to maintain the gut health of pets is attracting increasing attention. Summary of the Invention

[0008] To address the problems mentioned in the background art, the present invention aims to provide a functional pet food for regulating intestinal health and a method for preparing the same.

[0009] To solve the above-mentioned technical problems, the technical solution provided by the present invention is as follows:

[0010] A functional pet food for regulating gut health contains the following components in parts by weight: 20-30 parts sweet potato flour, 15-30 parts chicken meal, 5-15 parts enzymatically hydrolyzed chicken, 2-8 parts chicken bone meal, 5-15 parts chicken fat, 5-13 parts starch, 0.5-1.5 parts chicken liver powder, 0.5-3.5 parts beet pulp, 0.2-0.8 parts fish oil, 0.1-0.2 parts flaxseed oil, 0.5-1.5 parts cellulose, 2-8 parts fruit and vegetable powder, 1-3 parts vitamin and mineral premix, 0.5-1 part yeast hydrolysate, 0.05-0.15 parts yucca powder, 0.01-0.1 parts fructooligosaccharides, 0.05-0.15 parts chicory root powder, 0.2-3 parts traditional Chinese medicine premix, and 0.01-0.03 parts compound probiotics.

[0011] The herbal premix consists of 5-15 parts ginseng, 5-15 parts atractylodes macrocephala, 5-15 parts licorice, 5-15 parts alisma plantago-aquatica, 5-15 parts hawthorn, 5-15 parts malt, 15-25 parts lotus seed, 15-25 parts yam, and 15-25 parts poria cocos.

[0012] The compound probiotics consist of Bacillus subtilis, Enterococcus faecalis, Lactobacillus plantarum, Bacillus coagulans, and Lactobacillus reuteri in a mass ratio of 1-2:0.5-1:2-4:0.5-1:2-3.

[0013] In one embodiment of the present invention, the starch is one or more of corn starch, tapioca starch, or potato starch; the fruits and vegetables are one or more of carrot powder, pumpkin powder, apple powder, pear powder, or alfalfa; and the grains are one or more of rice, corn, wheat, millet, oats, or quinoa.

[0014] In one embodiment of the present invention, the functional pet food contains the following components in parts by weight: 25 parts sweet potato flour, 22.5 parts chicken powder, 10 parts enzymatically hydrolyzed chicken, 5 parts chicken bone meal, 10 parts chicken oil, 9 parts starch, 1 part chicken liver powder, 2 parts beet pulp, 0.5 parts fish oil, 0.15 parts flaxseed oil, 1 part cellulose, 5 parts fruit and vegetable powder, 2 parts vitamin and mineral premix, 0.75 parts yeast hydrolysate, 0.1 parts yucca powder, 0.05 parts fructooligosaccharides, 0.1 parts chicory root powder, 1.6 parts traditional Chinese medicine premix, and 0.02 parts compound probiotics;

[0015] The herbal premix consists of 10 parts ginseng, 10 parts atractylodes macrocephala, 10 parts licorice, 10 parts alisma plantago-aquatica, 10 parts hawthorn, 10 parts malt, 20 parts lotus seed, 20 parts yam, and 20 parts poria cocos.

[0016] The compound probiotics consist of Bacillus subtilis, Enterococcus faecalis, Lactobacillus plantarum, Bacillus coagulans, and Lactobacillus reuteri in a mass ratio of 2:1:3:1:2.

[0017] In one embodiment of the present invention, the functional pet food contains the following components in parts by weight: 20 parts sweet potato flour, 30 parts chicken powder, 5 parts enzymatically hydrolyzed chicken, 2 parts chicken bone meal, 15 parts chicken oil, 13 parts starch, 0.5 parts chicken liver powder, 3.5 parts beet pulp, 0.2 parts fish oil, 0.2 parts flaxseed oil, 0.5 parts cellulose, 8 parts fruit and vegetable powder, 1 part vitamin and mineral premix, 1 part yeast hydrolysate, 0.05 parts yucca powder, 0.1 parts fructooligosaccharides, 0.05 parts chicory root powder, 3 parts traditional Chinese medicine premix, and 0.01 parts compound probiotics;

[0018] The herbal premix consists of 15 parts ginseng, 15 parts atractylodes macrocephala, 5 parts licorice, 5 parts alisma plantago-aquatica, 15 parts hawthorn, 15 parts malt, 15 parts lotus seed, 15 parts yam, and 25 parts poria cocos.

[0019] The compound probiotics consist of Bacillus subtilis, Enterococcus faecalis, Lactobacillus plantarum, Bacillus coagulans, and Lactobacillus reuteri in a mass ratio of 2:1:3:1:2.

[0020] A functional pet food for improving intestinal barrier function contains the following components in parts by weight: 20-30 parts sweet potato starch, 15-30 parts chicken meal, 5-15 parts enzymatically hydrolyzed chicken, 2-8 parts chicken bone meal, 5-15 parts chicken fat, 5-13 parts starch, 0.5-1.5 parts chicken liver powder, 0.5-3.5 parts beet pulp, 0.2-0.8 parts fish oil, 0.1-0.2 parts flaxseed oil, 0.5-1.5 parts cellulose, 2-8 parts fruit and vegetable powder, 1-3 parts vitamin and mineral premix, 0.5-1 part yeast hydrolysate, 0.05-0.15 parts yucca powder, 0.01-0.1 parts fructooligosaccharides, 0.05-0.15 parts chicory root powder, 0.2-3 parts traditional Chinese medicine premix, and 0.01-0.03 parts compound probiotics.

[0021] The herbal premix consists of 5-15 parts ginseng, 5-15 parts atractylodes macrocephala, 5-15 parts licorice, 5-15 parts alisma plantago-aquatica, 5-15 parts hawthorn, 5-15 parts malt, 15-25 parts lotus seed, 15-25 parts yam, and 15-25 parts poria cocos.

[0022] The compound probiotics consist of Bacillus subtilis, Enterococcus faecalis, Lactobacillus plantarum, Bacillus coagulans, and Lactobacillus reuteri in a mass ratio of 1-2:0.5-1:2-4:0.5-1:2-3.

[0023] In one embodiment of the present invention, the starch is one or more of corn starch, tapioca starch, or potato starch; the fruits and vegetables are one or more of carrot powder, pumpkin powder, apple powder, pear powder, or alfalfa; and the grains are one or more of rice, corn, wheat, millet, oats, or quinoa.

[0024] A functional pet food for improving constipation contains the following components in parts by weight: 20-30 parts sweet potato starch, 15-30 parts chicken meal, 5-15 parts enzymatically hydrolyzed chicken, 2-8 parts chicken bone meal, 5-15 parts chicken fat, 5-13 parts starch, 0.5-1.5 parts chicken liver powder, 0.5-3.5 parts beet pulp, 0.2-0.8 parts fish oil, 0.1-0.2 parts flaxseed oil, 0.5-1.5 parts cellulose, 2-8 parts fruit and vegetable powder, 1-3 parts vitamin and mineral premix, 0.5-1 part yeast hydrolysate, 0.05-0.15 parts yucca powder, 0.01-0.1 parts fructooligosaccharides, 0.05-0.15 parts chicory root powder, 0.2-3 parts traditional Chinese medicine premix, and 0.01-0.03 parts compound probiotics.

[0025] The herbal premix consists of 5-15 parts ginseng, 5-15 parts atractylodes macrocephala, 5-15 parts licorice, 5-15 parts alisma plantago-aquatica, 5-15 parts hawthorn, 5-15 parts malt, 15-25 parts lotus seed, 15-25 parts yam, and 15-25 parts poria cocos.

[0026] The compound probiotics consist of Bacillus subtilis, Enterococcus faecalis, Lactobacillus plantarum, Bacillus coagulans, and Lactobacillus reuteri in a mass ratio of 1-2:0.5-1:2-4:0.5-1:2-3.

[0027] In one embodiment of the present invention, the starch is one or more of corn starch, tapioca starch, or potato starch; the fruits and vegetables are one or more of carrot powder, pumpkin powder, apple powder, pear powder, or alfalfa; and the grains are one or more of rice, corn, wheat, millet, oats, or quinoa.

[0028] This invention provides a method for preparing the above-mentioned functional pet food, wherein the preparation method is as follows:

[0029] After the ingredients are prepared in step (1), the raw materials are crushed and passed through a 40-mesh sieve. Sweet potato flour, chicken powder, enzymatically hydrolyzed chicken, chicken bone meal, starch, beet pulp, cellulose, fruit and vegetable powder, yeast hydrolysate, yucca powder, fructooligosaccharides, chicory root powder, Chinese herbal medicine premix premix, and vitamin and mineral premix are added and mixed. The mixture is then fed into a mixer. The mixture is then subjected to puffing, drying and spraying processes. During spraying, chicken oil, fish oil, flaxseed oil, compound probiotic powder and chicken liver are added to obtain dog food pellets.

[0030] Step (2) involves cooling the dog food pellets, then sieving and packaging them.

[0031] A functional cat food for regulating gut health contains the following components by weight: 20-40 parts chicken meal, 10-20 parts sweet potato flour, 5-15 parts enzymatically hydrolyzed chicken, 4-10 parts chicken bone meal, 5-15 parts chicken fat, 5-13 parts bone glue, 5-13 parts starch, 0.5-1.5 parts chicken liver powder, 0.5-3.5 parts beet pulp, 0.4-1.2 parts fish oil, 0.4-1.2 parts flaxseed oil, 0.5-1.5 parts fish meal, 0.2-1.4 parts brewer's yeast, 1-2 parts fruit and vegetable powder, 0.05-0.15 parts collagen, and Viscel PF cellulose. 0.1-0.5 parts, 0.01-0.1 parts of fructooligosaccharides, 0.05-0.15 parts of chicory root powder, 1-3 parts of vitamin and mineral premix, 0.2-4 parts of traditional Chinese medicine premix, and 0.01-0.03 parts of compound probiotics;

[0032] The herbal premix consists of 5-15 parts ginseng, 5-15 parts atractylodes macrocephala, 5-15 parts licorice, 5-15 parts alisma plantago-aquatica, 5-15 parts hawthorn, 5-15 parts malt, 15-25 parts lotus seed, 15-25 parts yam, and 15-25 parts poria cocos.

[0033] The compound probiotics consist of Bacillus subtilis, Enterococcus faecalis, Lactobacillus plantarum, Bacillus coagulans, and Lactobacillus reuteri in a mass ratio of 1-2:0.5-1:2-4:0.5-1:2-3.

[0034] In one embodiment of the present invention, the starch is one or more of corn starch, tapioca starch, or potato starch; the fruits and vegetables are one or more of carrot powder, pumpkin powder, apple powder, pear powder, or alfalfa; and the grains are one or more of rice, corn, wheat, millet, oats, or quinoa.

[0035] In one embodiment of the present invention, the cat food contains the following components in parts by weight: 30 parts chicken meal, 15 parts sweet potato meal, 10 parts enzymatically hydrolyzed chicken, 7 parts chicken bone meal, 10 parts chicken fat, 9 parts bone glue, 9 parts starch, 1 part chicken liver powder, 2 parts beet pulp, 0.8 parts fish oil, 0.8 parts flaxseed oil, 1 part fish meal, 0.8 parts brewer's yeast, 1.5 parts fruit and vegetable powder, 0.1 parts collagen, 0.3 parts Viscel PF cellulose, 0.05 parts fructooligosaccharides, 0.1 parts chicory root powder, 2 parts vitamin and mineral premix, 2 parts traditional Chinese medicine premix, and 0.02 parts compound probiotics;

[0036] The herbal premix consists of 10 parts ginseng, 10 parts atractylodes macrocephala, 10 parts licorice, 10 parts alisma plantago-aquatica, 10 parts hawthorn, 10 parts malt, 20 parts lotus seed, 20 parts yam, and 20 parts poria cocos.

[0037] The compound probiotics consist of Bacillus subtilis, Enterococcus faecalis, Lactobacillus plantarum, Bacillus coagulans, and Lactobacillus reuteri in a mass ratio of 2:1:3:1:2.

[0038] In one embodiment of the present invention, the cat food contains the following components in parts by weight: 20 parts chicken meal, 20 parts sweet potato meal, 5 parts enzymatically hydrolyzed chicken, 10 parts chicken bone meal, 5 parts chicken oil, 13 parts bone glue, 5 parts starch, 1.5 parts chicken liver powder, 0.5 parts beet pulp, 1.2 parts fish oil, 0.4 parts flaxseed oil, 1.5 parts fish meal, 0.2 parts brewer's yeast, 2 parts fruit and vegetable powder, 0.05 parts collagen, 0.5 parts Viscel PF cellulose, 0.01 parts fructooligosaccharides, 0.15 parts chicory root powder, 1 part vitamin and mineral premix, 4 parts traditional Chinese medicine premix, and 0.01 parts compound probiotics;

[0039] The herbal premix consists of 15 parts ginseng, 15 parts atractylodes macrocephala, 5 parts licorice, 5 parts alisma plantago-aquatica, 15 parts hawthorn, 15 parts malt, 15 parts lotus seed, 15 parts yam, and 25 parts poria cocos.

[0040] The compound probiotics consist of Bacillus subtilis, Enterococcus faecalis, Lactobacillus plantarum, Bacillus coagulans, and Lactobacillus reuteri in a mass ratio of 2:1:3:1:2.

[0041] This invention provides a method for preparing the above-mentioned cat food, wherein the preparation method is as follows:

[0042] The raw materials are crushed and passed through a 40-mesh sieve. Chicken meal, sweet potato meal, enzymatically hydrolyzed chicken, chicken bone meal, bone glue, starch, beet pulp, fish meal, brewer's yeast, fruit and vegetable powder, collagen, cellulose Viscel PF, fructooligosaccharides, chicory root powder, Chinese herbal medicine premix, and vitamin and mineral premix are added and mixed. The mixture is then fed into a mixing unit. The mixture undergoes puffing, drying, and spraying processes in sequence. During spraying, chicken oil, fish oil, flaxseed oil, compound probiotic powder, and chicken liver are added to obtain cat food pellets.

[0043] A functional cat food designed to improve diarrhea contains the following components by weight: 20-40 parts chicken meal, 10-20 parts sweet potato flour, 5-15 parts enzymatically hydrolyzed chicken, 4-10 parts chicken bone meal, 5-15 parts chicken fat, 5-13 parts bone glue, 5-13 parts starch, 0.5-1.5 parts chicken liver powder, 0.5-3.5 parts beet pulp, 0.4-1.2 parts fish oil, 0.4-1.2 parts flaxseed oil, 0.5-1.5 parts fish meal, 0.2-1.4 parts brewer's yeast, 1-2 parts fruit and vegetable powder, 0.05-0.15 parts collagen, and Viscel PF cellulose. 0.1-0.5 parts, 0.01-0.1 parts of fructooligosaccharides, 0.05-0.15 parts of chicory root powder, 1-3 parts of vitamin and mineral premix, 0.2-4 parts of traditional Chinese medicine premix, and 0.01-0.03 parts of compound probiotics;

[0044] The herbal premix consists of 5-15 parts ginseng, 5-15 parts atractylodes macrocephala, 5-15 parts licorice, 5-15 parts alisma plantago-aquatica, 5-15 parts hawthorn, 5-15 parts malt, 15-25 parts lotus seed, 15-25 parts yam, and 15-25 parts poria cocos.

[0045] The compound probiotics consist of Bacillus subtilis, Enterococcus faecalis, Lactobacillus plantarum, Bacillus coagulans, and Lactobacillus reuteri in a mass ratio of 1-2:0.5-1:2-4:0.5-1:2-3.

[0046] In one embodiment of the present invention, the starch is one or more of corn starch, tapioca starch, or potato starch; the fruits and vegetables are one or more of carrot powder, pumpkin powder, apple powder, pear powder, or alfalfa; and the grains are one or more of rice, corn, wheat, millet, oats, or quinoa.

[0047] The beneficial effects achieved by this invention are as follows:

[0048] This invention's functional pet food contains the following traditional Chinese medicine ingredients: ginseng, atractylodes macrocephala, licorice, alisma plantago-aquatica, hawthorn, lotus seed, yam, malt, and poria cocos. Ginseng is the principal ingredient, as it greatly replenishes vital energy and strengthens the spleen and stomach; atractylodes macrocephala invigorates the spleen and qi, dries dampness, and harmonizes the middle jiao; poria cocos is sweet and bland, invigorating the spleen and eliminating dampness; yam and lotus seed invigorate the spleen and stop diarrhea, serving as assistant ingredients; hawthorn eliminates food stagnation and disperses blood stasis, used for food stagnation from meat; malt stimulates appetite and aids digestion, used for food stagnation from wheat; alisma plantago-aquatica promotes urination and eliminates dampness, used for diarrhea, serving as adjuvant ingredients; licorice assists ginseng, atractylodes macrocephala, and poria cocos in invigorating qi, strengthening the spleen, and nourishing the stomach, while also harmonizing the other ingredients. The entire formula is mild in nature, combining tonification and elimination, with elimination within tonification, collectively achieving the effect of strengthening the spleen and harmonizing the stomach.

[0049] Furthermore, the functional pet food of this invention is fortified with fructooligosaccharides and chicory root powder. Fructooligosaccharides and chicory root powder can serve as fermentation carbon sources for intestinal flora, and can also increase the number of Bifidobacteria and Lactobacilli in the intestines. These two bacteria can produce short-chain fatty acids acetic acid and lactic acid by degrading carbohydrates, providing an energy source for host cells. These short-chain unsaturated fatty acids play an important role in maintaining the normal metabolism and integrity of intestinal cells, and may enhance the body's immune function. Fructooligosaccharides and chicory root powder can also exert an inhibitory effect by preventing pathogenic bacteria and toxins from adhering to epithelial cells.

[0050] This invention provides a functional pet food supplemented with Bacillus subtilis, Enterococcus faecalis, Lactobacillus plantarum, Bacillus coagulans, and Lactobacillus reuteri. Adding these strains to pet food can regulate the balance of the pet's intestinal flora, increase the abundance of beneficial bacteria, and improve the digestibility of nutrients. For example, adding compound probiotics to dog food can help prevent pathogen invasion and strengthen the intestinal barrier. The five strains in this invention can produce various nutrients and antibacterial substances such as bacteriocins, organic acids, enzymes, and short-chain fatty acids, as well as growth factors. These beneficial substances can inhibit the growth of harmful bacteria in the animal's intestines, improve the digestibility of nutrients, and thus improve the animal's intestinal health.

[0051] This invention provides a functional pet food that improves canine fecal scores, increases MTL levels in canine serum within the physiological range, reduces DAO activity, D-lactic acid, and LPS levels in canine serum, enhances intestinal mucosal barrier function, and significantly improves canine intestinal health.

[0052] The functional pet food of this invention can increase the average daily fecal weight of constipated dogs, shorten the time of excretion of the first activated charcoal, shorten the gastrointestinal transit time, effectively improve constipation, and the effect is significantly better than some products on the market.

[0053] Furthermore, the palatability optimization design and non-burdensome metabolic characteristics of the functional pet food of this invention enable pets to consume it safely for a long time, providing a convenient and efficient preventive health management solution for family pet owners.

[0054] This invention provides a new solution for treating feline diarrhea by improving symptoms of diarrhea in cats and regulating the intestinal flora structure, restoring the composition and abundance of the flora to the level of normal healthy cats.

[0055] In the gut microecological environment, the gut microbiota constitutes the biological barrier of the intestines and plays a vital role in the health of pets. The types and quantities of bacteria determine the balance of this micro-ecosystem. When a pet's physiology changes, or due to the effects of medication, some members of the normal microbiota are attacked or eliminated, which disrupts the balance of the normal microbiota, causing the normal combination of bacteria to transform into an abnormal combination; this is called dysbiosis. When the balance of the gut microecology is disrupted, the number of pathogenic bacteria increases, producing large amounts of endotoxins and putrefactive substances, which can cause symptoms such as acute and chronic diarrhea and constipation. The functional pet food of this invention has good effects in treating both diarrhea and constipation, and has a bidirectional regulatory effect in regulating gut microbiota imbalance.

[0056] The various components of this invention work synergistically to provide balanced nutrition. In addition to improving the intestinal health of pets, it is beneficial to maintaining the overall health of pets. The preparation method is simple, it does not contain preservatives or additives, it is safe and reliable, and has good prospects for commercial application. Attached Figure Description

[0057] Figure 1 The effect on canine fecal scores, compared with the control group, & P < 0.05 && P < 0.01.

[0058] Figure 2 The effect on serum MTL levels, compared with the control group, & P < 0.05 && P < 0.01.

[0059] Figure 3 Effects on DAO activity in canine serum compared to the control group & P < 0.05 && P < 0.01.

[0060] Figure 4 Effects on D-lactic acid levels in canine serum compared to the control group & P < 0.05 && P < 0.01.

[0061] Figure 5 The effect of LPS levels in canine serum compared to the control group. & P < 0.05 && P < 0.01.

[0062] Figure 6 Veen diagram of canine gut microbiota.

[0063] Figure 7 A horizontal species diagram of canine gut microbiota.

[0064] Figure 8 Analysis of canine gut microbiota using LEfSe.

[0065] Figure 9 Cat feces scoring criteria.

[0066] Figure 10 Veen diagram of the feline gut microbiota.

[0067] Figure 11 LEfSe analysis of feline gut microbiota. Detailed Implementation

[0068] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0069] It should be understood that the terminology used in this invention is merely for describing particular embodiments and is not intended to limit the invention. Furthermore, with respect to numerical ranges in this invention, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. Every smaller range between any stated value or intermediate value within a stated range, and any other stated value or intermediate value within said range, is also included in this invention. The upper and lower limits of these smaller ranges may be independently included or excluded from the range.

[0070] Unless otherwise specified, the experimental methods described in the following examples are conventional methods; unless otherwise specified, the reagents and materials are commercially available.

[0071] Examples 1-5: Vitamin and mineral premixes were purchased from Shandong Haichuang Industry and Trade Co., Ltd., and the raw materials were: choline chloride, potassium chloride, sodium chloride, potassium dihydrogen phosphate, L-lysine hydrochloride, manganese sulfate, zinc sulfate, ferrous sulfate, copper sulfate, calcium iodate, sodium selenite, vitamin A acetate, vitamin D3, dl-α-tocopherol acetate, thiamine nitrate (vitamin B1), riboflavin (vitamin B2), pyridoxine hydrochloride (vitamin B6), cyanocobalamin (vitamin B12), D-calcium pantothenate, nicotinamide, folic acid, D-biotin, and glutamine.

[0072] Examples 6-9: Vitamin and mineral premixes were purchased from Shandong Haichuang Industry and Trade Co., Ltd., and the raw materials were: taurine, L-lysine, DL-methionine, vitamin A acetate, vitamin D3, dl-α-tocopherol acetate, thiamine nitrate (vitamin B1), riboflavin (vitamin B2), niacin, D-calcium pantothenate, folic acid, pyridoxine hydrochloride (vitamin B6), cyanocobalamin (vitamin B12), D-biotin, choline chloride, calcium hydrogen phosphate, potassium chloride, sodium chloride, glycine iron complex (chelate), glycine copper complex (chelate), methionine manganese complex (chelate), zinc glycine, and calcium iodate.

[0073] Example 1: A functional pet food for regulating gut health, comprising the following components in parts by weight:

[0074] 25 parts sweet potato starch, 22.5 parts chicken meal, 10 parts enzymatically hydrolyzed chicken, 5 parts chicken bone meal, 10 parts chicken fat, 9 parts starch, 1 part chicken liver powder, 2 parts beet pulp, 0.5 parts fish oil, 0.15 parts flaxseed oil, 1 part cellulose, 5 parts fruit and vegetable powder, 2 parts vitamin and mineral premix, 0.75 parts yeast hydrolysate, 0.1 parts yucca powder, 0.05 parts fructooligosaccharides, 0.1 parts chicory root powder, 1.6 parts traditional Chinese medicine premix, and 0.02 parts compound probiotics.

[0075] The herbal premix consists of 10 parts ginseng, 10 parts atractylodes macrocephala, 10 parts licorice, 10 parts alisma plantago-aquatica, 10 parts hawthorn, 10 parts malt, 20 parts lotus seed, 20 parts yam, and 20 parts poria cocos.

[0076] The compound probiotics consist of Bacillus subtilis, Enterococcus faecalis, Lactobacillus plantarum, Bacillus coagulans, and Lactobacillus reuteri in a mass ratio of 2:1:3:1:2.

[0077] The starch is corn starch, tapioca starch, and potato starch in a mass ratio of 1:1:1; the fruit and vegetable powder is carrot powder, apple powder, pear powder, and alfalfa in a mass ratio of 1:1:1:1; and the grain is millet, oats, and quinoa in a mass ratio of 1:1:1.

[0078] The preparation method includes the following steps:

[0079] After the ingredients are prepared in step (1), the raw materials are crushed and passed through a 40-mesh sieve. Sweet potato flour, chicken powder, enzymatically hydrolyzed chicken, chicken bone meal, starch, beet pulp, cellulose, fruit and vegetable powder, yeast hydrolysate, yucca powder, fructooligosaccharides, chicory root powder, Chinese herbal medicine premix premix, and vitamin and mineral premix are added and mixed. The mixture is then fed into a mixer. The mixture is then subjected to puffing, drying and spraying processes. During spraying, chicken oil, fish oil, flaxseed oil, compound probiotic powder and chicken liver are added to obtain dog food pellets.

[0080] Step (2) involves cooling the dog food pellets, then sieving and packaging them.

[0081] Example 2 A functional pet food for regulating gut health, comprising the following components in parts by weight:

[0082] 20 parts sweet potato starch, 30 parts chicken powder, 5 parts enzymatically hydrolyzed chicken, 2 parts chicken bone meal, 15 parts chicken fat, 13 parts starch, 0.5 parts chicken liver powder, 3.5 parts beet pulp, 0.2 parts fish oil, 0.2 parts flaxseed oil, 0.5 parts cellulose, 8 parts fruit and vegetable powder, 1 part vitamin and mineral premix, 1 part yeast hydrolysate, 0.05 parts yucca powder, 0.1 parts fructooligosaccharides, 0.05 parts chicory root powder, 3 parts traditional Chinese medicine premix, and 0.01 parts compound probiotics.

[0083] The herbal premix consists of 15 parts ginseng, 15 parts atractylodes macrocephala, 5 parts licorice, 5 parts alisma plantago-aquatica, 15 parts hawthorn, 15 parts malt, 15 parts lotus seed, 15 parts yam, and 25 parts poria cocos.

[0084] The compound probiotics consist of Bacillus subtilis, Enterococcus faecalis, Lactobacillus plantarum, Bacillus coagulans, and Lactobacillus reuteri in a mass ratio of 2:1:3:1:2.

[0085] The starch is corn starch, tapioca starch, and potato starch in a mass ratio of 1:1:1; the fruit and vegetable powder is carrot powder, pumpkin powder, apple powder, and alfalfa in a mass ratio of 1:1:1:1; and the grain is wheat, millet, and quinoa in a mass ratio of 1:1:1.

[0086] The preparation method is the same as in Example 1.

[0087] Example 3 A functional pet food for regulating gut health, comprising the following components in parts by weight:

[0088] 30 parts sweet potato starch, 15 parts chicken meal, 15 parts enzymatically hydrolyzed chicken, 8 parts chicken bone meal, 5 parts chicken fat, 5 parts starch, 1.5 parts chicken liver powder, 0.5 parts beet pulp, 0.8 parts fish oil, 0.1 parts flaxseed oil, 1.5 parts cellulose, 2 parts fruit and vegetable powder, 3 parts vitamin and mineral premix, 0.5 parts yeast hydrolysate, 0.15 parts yucca powder, 0.01 parts fructooligosaccharides, 0.05 parts chicory root powder, 0.2 parts traditional Chinese medicine premix, and 0.03 parts compound probiotics.

[0089] The herbal premix consists of 5 parts ginseng, 5 parts atractylodes macrocephala, 15 parts licorice, 15 parts alisma plantago-aquatica, 5 parts hawthorn, 5 parts malt, 25 parts lotus seed, 25 parts yam, and 15 parts poria cocos.

[0090] The compound probiotics consist of Bacillus subtilis, Enterococcus faecalis, Lactobacillus plantarum, Bacillus coagulans, and Lactobacillus reuteri in a mass ratio of 1:0.5:2:0.5:3.

[0091] The starch is corn starch and potato starch in a mass ratio of 1:1; the fruit and vegetable powder is carrot powder, pumpkin powder, apple powder, and pear powder in a mass ratio of 1:1:1:1; the grain is rice, corn, and oats in a mass ratio of 1:1:1.

[0092] The preparation method is the same as in Example 1.

[0093] Example 4 A functional pet food for regulating gut health, comprising the following components in parts by weight:

[0094] 18 parts sweet potato starch, 25 parts chicken meal, 5 parts enzymatically hydrolyzed chicken, 8 parts chicken bone meal, 14 parts chicken fat, 9 parts starch, 1.5 parts chicken liver powder, 2.3 parts beet pulp, 0.3 parts fish oil, 0.1 parts flaxseed oil, 1.4 parts cellulose, 3.5 parts fruit and vegetable powder, 1.5 parts vitamin and mineral premix, 1 part yeast hydrolysate, 0.12 parts yucca powder, 0.04 parts fructooligosaccharides, 0.13 parts chicory root powder, 2.2 parts traditional Chinese medicine premix, and 0.03 parts compound probiotics.

[0095] The herbal premix consists of 8 parts ginseng, 5 parts atractylodes macrocephala, 10 parts licorice, 13 parts alisma plantago-aquatica, 6 parts hawthorn, 10 parts malt, 21 parts lotus seed, 21 parts yam, and 15 parts poria cocos.

[0096] The compound probiotics consist of Bacillus subtilis, Enterococcus faecalis, Lactobacillus plantarum, Bacillus coagulans, and Lactobacillus reuteri in a mass ratio of 2:1:3:0.5:2.

[0097] The starch is tapioca starch and potato starch in a mass ratio of 1:1; the fruit and vegetable powder is pumpkin powder, apple powder, pear powder, and alfalfa in a mass ratio of 1:1:1:1; the grains are rice, corn, wheat, millet, and quinoa in a mass ratio of 1:1:1:1:1.

[0098] The preparation method is the same as in Example 1.

[0099] Example 5 A functional pet food for regulating gut health, comprising the following components in parts by weight:

[0100] 27 parts sweet potato starch, 20 parts chicken meal, 10 parts enzymatically hydrolyzed chicken, 3 parts chicken bone meal, 9 parts chicken fat, 11 parts starch, 1.5 parts chicken liver powder, 3 parts beet pulp, 0.5 parts fish oil, 0.1 parts flaxseed oil, 1 part cellulose, 3 parts fruit and vegetable powder, 1.4 parts vitamin and mineral premix, 0.9 parts yeast hydrolysate, 0.1 parts yucca powder, 0.06 parts fructooligosaccharides, 0.12 parts chicory root powder, 2.6 parts traditional Chinese medicine premix, and 0.02 parts compound probiotics.

[0101] The herbal premix consists of 15 parts ginseng, 5 parts atractylodes macrocephala, 5 parts licorice, 5 parts alisma plantago-aquatica, 10 parts hawthorn, 15 parts malt, 25 parts lotus seeds, 20 parts yam, and 10 parts poria cocos.

[0102] The compound probiotics consist of Bacillus subtilis, Enterococcus faecalis, Lactobacillus plantarum, Bacillus coagulans, and Lactobacillus reuteri in a mass ratio of 1:0.5:4:1:2.

[0103] The starch is corn starch, tapioca starch, and potato starch in a mass ratio of 1:1:1; the fruit and vegetable powder is carrot powder, apple powder, pear powder, and alfalfa in a mass ratio of 1:1:1:1; and the grain is wheat, oats, and quinoa in a mass ratio of 1:1:1.

[0104] The preparation method is the same as in Example 1.

[0105] Example 6 A functional cat food for regulating gut health, comprising the following components in parts by weight:

[0106] 20 parts chicken meal, 20 parts sweet potato meal, 5 parts enzymatically hydrolyzed chicken, 10 parts chicken bone meal, 5 parts chicken fat, 13 parts bone glue, 5 parts starch, 1.5 parts chicken liver powder, 0.5 parts beet pulp, 1.2 parts fish oil, 0.4 parts flaxseed oil, 1.5 parts fish meal, 0.2 parts brewer's yeast, 2 parts fruit and vegetable powder, 0.05 parts collagen, 0.5 parts Viscel PF cellulose, 0.01 parts fructooligosaccharides, 0.15 parts chicory root powder, 1 part vitamin and mineral premix, 4 parts traditional Chinese medicine premix, and 0.01 parts compound probiotics.

[0107] The herbal premix consists of 15 parts ginseng, 15 parts atractylodes macrocephala, 5 parts licorice, 5 parts alisma plantago-aquatica, 15 parts hawthorn, 15 parts malt, 15 parts lotus seed, 15 parts yam, and 25 parts poria cocos.

[0108] The compound probiotics consist of Bacillus subtilis, Enterococcus faecalis, Lactobacillus plantarum, Bacillus coagulans, and Lactobacillus reuteri in a mass ratio of 2:1:3:1:2.

[0109] The starch is corn starch, tapioca starch, and potato starch in a mass ratio of 1:1:1; the fruit and vegetable powder is carrot powder, apple powder, pear powder, and pumpkin powder in a mass ratio of 1:1:1:1; and the grain is millet, oats, and quinoa in a mass ratio of 1:1:1.

[0110] Preparation method:

[0111] The raw materials are crushed and passed through a 40-mesh sieve. Chicken meal, sweet potato meal, enzymatically hydrolyzed chicken, chicken bone meal, bone glue, starch, beet pulp, fish meal, brewer's yeast, fruit and vegetable powder, collagen, cellulose Viscel PF, fructooligosaccharides, chicory root powder, Chinese herbal medicine premix, and vitamin and mineral premix are added and mixed. The mixture is then fed into a mixing unit. The mixture undergoes puffing, drying, and spraying processes in sequence. During spraying, chicken oil, fish oil, flaxseed oil, compound probiotic powder, and chicken liver are added to obtain cat food pellets.

[0112] Example 7 A functional cat food for regulating gut health, comprising the following components in parts by weight:

[0113] 40 parts chicken meal, 10 parts sweet potato flour, 15 parts enzymatically hydrolyzed chicken, 4 parts chicken bone meal, 15 parts chicken oil, 5 parts bone glue, 13 parts starch, 0.5 parts chicken liver powder, 3.5 parts beet pulp, 0.4 parts fish oil, 1.2 parts flaxseed oil, 0.5 parts fish meal, 1.4 parts brewer's yeast, 1 part fruit and vegetable powder, 0.15 parts collagen, 0.1 parts Viscel PF cellulose, 0.1 parts fructooligosaccharides, 0.05 parts chicory root powder, 3 parts vitamin and mineral premix, 0.2 parts traditional Chinese medicine premix, and 0.03 parts compound probiotics.

[0114] The herbal premix consists of 5 parts ginseng, 5 parts atractylodes macrocephala, 15 parts licorice, 15 parts alisma plantago-aquatica, 5 parts hawthorn, 5 parts malt, 25 parts lotus seed, 25 parts yam, and 15 parts poria cocos.

[0115] The compound probiotics consist of Bacillus subtilis, Enterococcus faecalis, Lactobacillus plantarum, Bacillus coagulans, and Lactobacillus reuteri in a mass ratio of 1:0.5:2:0.5:3.

[0116] The starch is tapioca starch and potato starch in a mass ratio of 1:1; the fruit and vegetable powder is pumpkin powder, apple powder, pear powder, and alfalfa in a mass ratio of 1:1:1:1; the grains are rice, corn, wheat, millet, and quinoa in a mass ratio of 1:1:1:1:1.

[0117] The preparation method is described in Example 6.

[0118] Example 8 A functional cat food for regulating gut health, comprising the following components in parts by weight:

[0119] Chicken meal 33 parts, sweet potato meal 11 parts, enzymatically hydrolyzed chicken 13 parts, chicken bone meal 8 parts, chicken oil 10 parts, bone glue 10 parts, starch 12 parts, chicken liver meal 0.8 parts, beet pulp 3 parts, fish oil 1 part, flaxseed oil 1 part, fish meal 1.3 parts, brewer's yeast 0.9 parts, fruit and vegetable powder 1.7 parts, collagen 0.15 parts, cellulose Viscel PF 0.3 parts, fructooligosaccharides 0.08 parts, chicory root powder 0.12 parts, vitamin and mineral premix 2.4 parts, traditional Chinese medicine premix 3 parts, compound probiotics 0.03 parts.

[0120] The herbal premix consists of 8 parts ginseng, 5 parts atractylodes macrocephala, 10 parts licorice, 13 parts alisma plantago-aquatica, 6 parts hawthorn, 10 parts malt, 21 parts lotus seed, 21 parts yam, and 15 parts poria cocos.

[0121] The compound probiotics consist of Bacillus subtilis, Enterococcus faecalis, Lactobacillus plantarum, Bacillus coagulans, and Lactobacillus reuteri in a mass ratio of 2:1:3:0.5:2.

[0122] The starch is corn starch, tapioca starch, and potato starch in a mass ratio of 1:1:1; the fruit and vegetable powder is carrot powder, apple powder, pear powder, and alfalfa in a mass ratio of 1:1:1:1; and the grain is wheat, oats, and quinoa in a mass ratio of 1:1:1.

[0123] The preparation method is described in Example 6.

[0124] Example 9 A functional cat food for regulating gut health, comprising the following components in parts by weight:

[0125] 30 parts chicken meal, 15 parts sweet potato flour, 10 parts enzymatically hydrolyzed chicken, 7 parts chicken bone meal, 10 parts chicken oil, 9 parts bone glue, 9 parts starch, 1 part chicken liver powder, 2 parts beet pulp, 0.8 parts fish oil, 0.8 parts flaxseed oil, 1 part fish meal, 0.8 parts brewer's yeast, 1.5 parts fruit and vegetable powder, 0.1 parts collagen, 0.3 parts Viscel PF cellulose, 0.05 parts fructooligosaccharides, 0.1 parts chicory root powder, 2 parts vitamin and mineral premix, 2 parts traditional Chinese medicine premix, and 0.02 parts compound probiotics.

[0126] The herbal premix consists of 10 parts ginseng, 10 parts atractylodes macrocephala, 10 parts licorice, 10 parts alisma plantago-aquatica, 10 parts hawthorn, 10 parts malt, 20 parts lotus seed, 20 parts yam, and 20 parts poria cocos.

[0127] The compound probiotics consist of Bacillus subtilis, Enterococcus faecalis, Lactobacillus plantarum, Bacillus coagulans, and Lactobacillus reuteri in a mass ratio of 2:1:3:1:2.

[0128] The starch is corn starch, tapioca starch, and potato starch in a mass ratio of 1:1:1; the fruit and vegetable powder is carrot powder, pumpkin powder, apple powder, and alfalfa in a mass ratio of 1:1:1:1; and the grain is wheat, millet, and quinoa in a mass ratio of 1:1:1.

[0129] The preparation method is described in Example 6.

[0130] Comparative Example 1

[0131] The only difference between its composition and that of Example 1 is that it does not contain prebiotics or probiotics, and the amount of prebiotics and probiotics reduced is increased in the Chinese herbal premix.

[0132] Comparative Example 2

[0133] The only difference between its components and those of Example 1 is the change in the composition of the probiotics. Bifidobacterium lactis is used instead of Bacillus subtilis, Lactobacillus acidophilus is used instead of Lactobacillus plantarum, and Saccharomyces boulardii is used instead of Lactobacillus reuteri.

[0134] Comparative Example 3

[0135] The only difference between its components and those of Example 1 is the change in the composition of the probiotics: Pseudococcus lactis replaces Enterococcus faecium, Bifidobacterium longum replaces Lactobacillus plantarum, and Bifidobacterium animalis replaces Lactobacillus reuteri.

[0136] Comparative Example 4

[0137] The only difference between its composition and that of Example 1 is that it does not contain Chinese herbal medicine premix, and the reduced amount is proportionally increased in prebiotics and probiotics.

[0138] Comparative Example 5

[0139] The only difference between its components and those of Example 1 is the change in the composition of probiotics and prebiotics. Resistant dextrin is used instead of fructooligosaccharides, Lactobacillus rhamnosus is used instead of Bacillus subtilis, and Pediococcus lactis is used instead of Enterococcus faecium.

[0140] Comparative Example 6

[0141] The formulation of Example 1 disclosed in Invention Patent CN121128823A (A prescription food for pet dogs suffering from acute enteritis and its preparation method) is as follows: 15 parts rice, 10 parts barley, 15 parts chicken meal, 20 parts corn gluten meal, 10 parts cassava, 10 parts sweet potato, 3 parts chicken fat, 5 parts fish oil, 0.4 parts lactic acid, 4 parts chicken liver powder, 0.01 parts glutamine, 3 parts beet pulp, 0.5 parts salt, 0.4 parts fructooligosaccharides, 1 part chicory, 0.3 parts licorice, 0.3 parts orange peel, 0.03 parts rosemary extract, 0.3 parts tea polyphenols, 0.3 parts cranberry, 0.6 parts probiotics, and 1.5 parts premix.

[0142] Comparative Example 7

[0143] Commercially available Shiba Inu complete hypoallergenic formula dog food.

[0144] Comparative Example 8

[0145] Commercially available Yue Shi Traditional Chinese Medicine Complete Dog Food (for constipation) Herbal Formula.

[0146] Comparative Example 9

[0147] The only difference between its components and those of Example 9 is the change in the composition of probiotics and prebiotics, replacing chicory root powder with xylooligosaccharides and replacing Bacillus subtilis with Lactobacillus rhamnosus.

[0148] Comparative Example 10

[0149] The only difference between its components and those of Example 9 is the change in the composition of probiotics and prebiotics, replacing fructooligosaccharides with maltodextrin and replacing Lactobacillus plantarum with Lactobacillus acidophilus.

[0150] Comparative Example 11

[0151] The formulation disclosed in Example 1 of the invention patent CN118178535A (A composition of probiotics and natural plants and its application in the preparation of products for preventing and treating diarrhea in dogs and cats) is as follows: 60 parts of dried plum, 20 parts of gallnut, 60 parts of yam, 60 parts of atractylodes macrocephala, 8 parts of ginseng, 50 parts of brewer's yeast, 60 parts of Bacillus subtilis, and 80 parts of Enterococcus faecalis.

[0152] Example 1: Test on the effect of the functional pet food of the present invention on improving canine intestinal function

[0153] 1. Experimental Methods

[0154] Twenty-four healthy beagles were selected and kept in the animal house for two weeks for acclimatization. They were then randomly divided into a control group, Example 1 group, Example 2 group, and Example 3 group, with six dogs in each group.

[0155] The control group was fed regular dog food; the Example 1 group was fed the Example 1 functional pet food; the Example 2 group was fed the Example 2 functional pet food; and the Example 3 group was fed the Example 3 functional pet food. The regular dog food was purchased from Keao Xieli (Tianjin) Feed Co., Ltd. All groups of dogs received the same daily feeding amount. Feeding continued for 28 days.

[0156] 2. Detection indicators

[0157] 2.1 On the 28th day of the trial period, two researchers quickly scored the feces of each dog on-site according to the fecal scoring criteria and obtained the average score.

[0158] 1 point (hard like a bullet, crumbles easily under pressure); 1.5 points (hard and dry, cracks when pressed); 2 points (well-formed, leaves no mark when picked up); 2.5 points (well-formed, slightly moist, leaves a mark when picked up); 3 points (moist, somewhat loose, leaves a noticeable mark when picked up); 3.5 points (very moist, but still retains some shape); 4 points (viscous liquid with some solids); 4.5 points (slightly viscous liquid); 5 points (watery liquid). The ideal score is 2.5 points.

[0159] 2.2 Determination of serum gastrointestinal hormone levels

[0160] The serum motilin (MTL) content was detected by ELISA.

[0161] 2.3 Determination of serum intestinal barrier function indicators

[0162] The activity of diamine oxidase (DAO) and the levels of D-lactic acid and endotoxin (LPS) in serum were detected by ELISA.

[0163] 3. Statistical Methods

[0164] Data processing was performed using GraphPad Prism 7.0 software, with the measurement data presented in... ±s indicates that the comparison between groups was performed using one-way ANOVA, and P<0.05 was considered statistically significant.

[0165] 4. Experimental Results

[0166] 4.1 Effect on canine fecal scores

[0167] Table 1 Comparison of fecal scores among different groups of dogs

[0168] Note: Compared with the control group, & P < 0.05 && P < 0.01.

[0169] The results are shown in Table 1. Figure 1 Compared with the control group, the fecal scores of dogs in Example 1, Example 2, and Example 3 groups showed significant differences.

[0170] 4.2 Effect on serum MTL levels

[0171] The results are shown in Table 2. Figure 2 Compared with the control group, the serum MTL content in dogs in Example 1, Example 2, and Example 3 groups was significantly increased. The functional pet food of this invention can increase serum MTL content within the physiological range.

[0172] Table 2 Comparison of MTL content in serum of dogs in each group

[0173] Note: Compared with the control group, & P < 0.05 && P < 0.01.

[0174] 4.3 Effects on serum intestinal barrier function indicators

[0175] See results Figure 3 , Figure 4 , Figure 5 Compared with the control group, the DAO activity, D-lactic acid and LPS content in the serum of dogs in the Example 1, Example 2 and Example 3 groups were significantly reduced.

[0176] Gastrointestinal motility, especially migrating complex (MMC), promotes the digestion and absorption of nutrients in the digestive tract and is mainly regulated by the enteric nervous system and gastrointestinal hormones. MLT can stimulate the occurrence and migration of MMC and is one of the important initiating factors for phase III of MMC. DAO is an enzyme in the villous cells of the upper intestinal mucosa. After damage to the gastrointestinal mucosa, a large amount of DAO is released into the intestinal lumen and reaches the bloodstream through the intercellular spaces of the intestinal wall and lymphatic reflux. Plasma DAO activity is closely related to the integrity and maturity of intestinal mucosal epithelial cells. D-lactic acid is a metabolic product of intestinal bacteria. After intestinal mucosal damage, a large amount of D-lactic acid in the intestinal lumen enters the bloodstream through the damaged site, increasing the blood D-lactic acid content. Therefore, elevated blood D-lactic acid levels often indicate intestinal mucosal damage and increased permeability. LPS is a component of the cell wall of Gram-negative bacteria. Current research suggests that LPS has a smaller molecular weight than bacteria and can more easily cross the intestinal mucosal barrier. Damage to the intestinal mucosal barrier can lead to elevated blood LPS levels.

[0177] The experimental results show that the functional pet food of this invention improves canine fecal scores, increases the MTL content in canine serum within the physiological range, reduces DAO activity, D-lactic acid and LPS content in canine serum, enhances intestinal mucosal barrier function, and significantly improves canine intestinal health.

[0178] Example 2: Study on the effects of the functional pet food of the present invention on canine gut health

[0179] 1. Test Sample: Functional dog food from Example 1.

[0180] 2. Experimental Animals and Experimental Design

[0181] 2.1 Experimental Design

[0182] The experiment lasted 28 days. During the experiment, 20 healthy adult dogs, weighing 4.2-0.34 kg and randomly assigned to sex, were selected and divided into two groups of 10 dogs each. One group (experimental group) was fed the functional dog food described in Example 1, while the other group (control group) was fed the control dog food. Each dog was housed individually in a pet cage (1.6m x 0.7m x 0.7m). Necessary immunizations and deworming were performed before the experiment. Dog litter was changed daily, and the pens were cleaned and maintained to ensure cleanliness. During the experiment, each dog in the experimental group was fed 200g of dog food and 500mL of water at 8:30 AM daily. The feeding status and mental state of all pets were observed during the experiment, as well as any adverse reactions such as aversion, diarrhea, or vomiting.

[0183] 2.2 Growth performance determination

[0184] The dogs were weighed on an empty stomach before the experiment and on day 28 of the experiment. The food intake and water consumption of the dogs were recorded before each morning feeding. At the end of the experiment, the average daily food intake and average daily water consumption of all dogs were calculated.

[0185] 2.3 Gut Health Assessment

[0186] Gut microbiome analysis

[0187] At the end of the experiment, fresh feces were collected for cryopreservation at -80°C. On day 28, three dogs from each group were sampled, and the samples were sent to Shanghai Meiji Biotechnology Co., Ltd. for 16S rDNA metagenomic sequencing using high-throughput sequencing technology. To study the species composition of each sample, OTUs (Operational Taxonomic Units) were clustered based on the Effective Tags of all samples with 97% identity, and then the OTU sequences were annotated for species. Based on the OTU results obtained from the clustering and the research needs, the common and unique OTUs among different samples (groups) were analyzed and plotted as Venn graphs.

[0188] To observe the differences in species richness among groups, the top ten most abundant species were selected and their gut microbiota differences were compared and analyzed at the phylum and genus levels using a T-test. Species with significant differences in abundance among groups at both the phylum and genus levels were specifically identified, and the enrichment of these differentially abundant species across different groups was determined to assess the significance of community structure differences between groups. LDA values ​​for species were calculated using LEfSe (LDAEffect Size) analysis, and differentially abundant species with LDA values ​​greater than 2 were selected. Bar charts and cladistic diagrams of LDA value distribution were then constructed to compare species differences at each level.

[0189] 3. Experimental Results

[0190] 3.1 The effect of functional dog food on the feeding performance of pet dogs

[0191] As shown in Table 3, when there was no significant difference in initial body weight, there was no significant difference in final body weight and daily food intake between the experimental group and the control group (P>0.05), but the average daily water consumption of the experimental group was significantly higher than that of the control group (P<0.05).

[0192] Table 3. Effects of Functional Dog Foods on Feeding Characteristics of Pet Dogs

[0193] 3.2 Effects of functional dog food on the gut microbiota of pet dogs

[0194] 3.3.1 Venn Diagram

[0195] Depend on Figure 6 It can be seen that the experimental group (A) and the control group (B) contain 77 and 29 OUTs respectively, with a total of 158 OTUs between the groups.

[0196] 3.3.2 Genus-level species composition

[0197] Depend on Figure 7 It was found that the level of Faecalibacterium in the experimental group was significantly higher than that in the control group (P<0.05), while there was no significant difference in the level of Canis genus (P>0.05).

[0198] 3.3.4 LEfSe Analysis

[0199] Depend on Figure 8 It was found that the canine intestinal test group showed significant enrichment of the genera *Oscillospirales*, *Ruminococcaceae*, *Faecalibacterium*, *Lachnospiraceae_NK4Al36_group*, *Holdemanella*, *Colidextribacte*, *norank_f_Erysipelotrichaceae*, *Parabacteroides*, and *Tannerellaceae*. The control group showed significant enrichment of the genera *Ruminococcus_g_navus_group*.

[0200] This study investigated the effects of functional dog food on canine gut health. After feeding dogs with functional dog food for 28 days, the following conclusions were drawn: feeding functional dog food did not affect the dogs' weight or food intake, but it did increase their water consumption; feeding functional dog food affected the canine gut microbiota species, increasing the gut microbiota species diversity index in the experimental group, and increasing the number and abundance of beneficial bacteria in the gut, thus better protecting the dogs' health.

[0201] Example 3: Effect Test of the Functional Pet Food of the Present Invention on Canine Constipation

[0202] 1. Experimental Methods

[0203] Fifty healthy Beagles were selected and acclimatized in the animal facility for two weeks. Each dog was given 5 sausages (15g each) and 3mg / kg of diphenoxylate daily. The diphenoxylate was encased in the sausage, and the dogs were instructed to chew and swallow it. If a dog refused, its neck could be held to assist swallowing. Water intake was not restricted. A slow-transit constipation model was established in approximately 14 days. The model dogs exhibited difficulty defecating, and their feces were dry, dark in color, and contained very little water.

[0204] Experimental dogs that had developed a constipation model were randomly divided into four groups according to their weight: a model control group, Example 1 group, Comparative Example 1 group, Comparative Example 2 group, Comparative Example 3 group, Comparative Example 4 group, Comparative Example 5 group, Comparative Example 6 group, Comparative Example 7 group, and Comparative Example 8 group, with five dogs in each group. The model control group was fed regular dog food; Example 1 group was fed the functional pet food of Example 1; Comparative Example 1 group was fed the product of Comparative Example 1; Comparative Example 2 group was fed the product of Comparative Example 2; Comparative Example 3 group was fed the product of Comparative Example 3; Comparative Example 4 group was fed the product of Comparative Example 4; Comparative Example 5 group was fed the product of Comparative Example 5; Comparative Example 6 group was fed the product of Comparative Example 6; Comparative Example 7 group was fed the product of Comparative Example 7; and Comparative Example 8 group was fed the product of Comparative Example 8. Regular dog food was purchased from Keao Xieli (Tianjin) Feed Co., Ltd. The daily feeding amount was the same for all groups of dogs.

[0205] After successful modeling, feeding began, with each group of dogs receiving the same daily feed amount for 14 consecutive days.

[0206] 2. Detection indicators

[0207] 2.1 The average daily fecal weight of the two groups of dogs was measured.

[0208] 2.2 The time of excretion of the first activated charcoal pellet was determined by the activated charcoal gavage method.

[0209] After fasting all dogs for 24 hours, add activated charcoal (10g / 100ml) to their food and record the time of eating and the first black stool.

[0210] 2.3 Measurement of gastrointestinal transit time

[0211] Twenty barium strips of three different shapes were administered to the animals at 0, 12, and 24 hours. Feces were collected from 0 hours onwards, and the shape and number of barium strips in the feces were counted until all barium strips were expelled. Gastrointestinal transit time (GITT) was defined as the time it took for 90% of the barium strips (18 strips) to be expelled. The average value measured for the three barium strip shapes was taken as the final value for each measurement.

[0212] 3. Statistical Methods

[0213] Data processing was performed using GraphPad Prism 7.0 software, with the measurement data presented in... ±s indicates that the comparison between groups was performed using one-way ANOVA, and P<0.05 was considered statistically significant.

[0214] 4. Experimental Results

[0215] 4.1 Effect on average daily fecal weight in dogs

[0216] Table 4 Comparison of average daily fecal weight among different groups of dogs

[0217] Note: Compared with the model control group, & P < 0.05 && P < 0.01; compared with Example 1 group, # P < 0.05 ## P < 0.01.

[0218] The results are shown in Table 4. Compared with the model control group, the average daily fecal weight of dogs in the Example 1 group was significantly increased, showing a statistically significant difference. Compared with Comparative Examples 1-5, the Example 1 group showed a statistically significant difference in increasing the average daily fecal weight of dogs. Compared with Comparative Examples 6, 7, and 8, the Example 1 group showed a statistically significant difference in increasing the average daily fecal weight of dogs.

[0219] 4.2 Effect on the first activated carbon excretion time in dogs

[0220] The results are shown in Table 5. Compared with the model control group, the excretion time of the first activated charcoal pellet in dogs in Example 1 group was significantly shortened, showing a statistically significant difference. Compared with Comparative Examples 1-5, Example 1 group showed a statistically significant difference in shortening the excretion time of the first activated charcoal pellet in dogs. Compared with Comparative Examples 6, 7, and 8, Example 1 group showed a statistically significant difference in shortening the excretion time of the first activated charcoal pellet in dogs.

[0221] Table 5 Comparison of the first activated charcoal excretion time in each group of dogs

[0222] Note: Compared with the model control group,& P < 0.05 && P < 0.01; compared with Example 1 group, # P < 0.05 ## P < 0.01.

[0223] 4.3 Effect on canine gastrointestinal transit time

[0224] The results are shown in Table 6. Compared with the model control group, the gastrointestinal transit time of dogs in Example 1 group was significantly shortened, showing a statistically significant difference. Compared with Comparative Examples 1-5, Example 1 group showed a statistically significant difference in shortening the gastrointestinal transit time of dogs. Compared with Comparative Examples 6, 7, and 8, Example 1 group showed a statistically significant difference in shortening the gastrointestinal transit time of dogs.

[0225] Table 6 Comparison of gastrointestinal transit time among different groups of dogs

[0226] Note: Compared with the model control group, & P < 0.05 && P < 0.01; compared with Example 1 group, # P < 0.05 ## P < 0.01.

[0227] Experimental results show that the functional pet food of this invention increases the average daily fecal weight of constipated dogs, shortens the time for the first activated charcoal to be excreted, shortens the gastrointestinal transit time, and effectively improves constipation.

[0228] The only difference between Comparative Example 1 and Example 1 is that it does not contain prebiotics or probiotics. Comparative Example 1 is less effective at improving canine constipation than Example 1. The only difference between Comparative Example 2 and Example 1 is the composition of the probiotics: Bifidobacterium lactis replaces Bacillus subtilis, Lactobacillus acidophilus replaces Lactobacillus plantarum, and Saccharomyces boulardii replaces Lactobacillus reuteri. Comparative Example 2 is less effective at improving canine constipation than Example 1. The only difference between Comparative Example 3 and Example 1 is the composition of the probiotics: Pediococcus lactis replaces Enterococcus faecium, Bifidobacterium longum replaces Lactobacillus plantarum, and Bifidobacterium animalis replaces Lactobacillus reuteri. Comparative Example 3 is less effective at improving canine constipation than Example 1. The only difference between Comparative Example 4 and Example 1 is that it does not contain herbal premix. Comparative Example 4 is less effective at improving canine constipation than Example 1. The only difference between Comparative Example 5 and Example 1 is the change in the composition of probiotics and prebiotics. Resistant dextrin was used instead of fructooligosaccharides, Lactobacillus rhamnosus was used instead of Bacillus subtilis, and Pediococcus lactis was used instead of Enterococcus faecium. Comparative Example 5 was less effective in improving canine constipation than Example 1. The results of Comparative Examples 1-5 show that, under the same dosage of functional pet food, reducing the amount of ingredients or changing the composition of the ingredients in the functional pet food reduces the effectiveness in improving canine constipation to varying degrees. This invention demonstrates a synergistic effect among the ingredients of the functional pet food.

[0229] Compared with Comparative Example 6, the functional pet food of the present invention is more effective in improving canine constipation, demonstrating significant technical advantages. Compared with Comparative Example 7, the functional pet food of the present invention is more effective in improving canine constipation, demonstrating significant technical advantages. Compared with Comparative Example 8, the functional pet food of the present invention is more effective in improving canine constipation, demonstrating significant technical advantages.

[0230] In summary, the functional pet food of this invention can increase the average daily fecal weight of constipated dogs, shorten the time of excretion of the first activated charcoal pellet, shorten the gastrointestinal transit time, effectively improve constipation, and its effect is significantly better than some market products.

[0231] Example 4: Safety Test of the Cat Food of the Invention

[0232] Nine cats of various breeds were selected for the test, including Golden Shaded, Tabby, Blue and White Munchkin, and American Shorthair with white markings. The number of males and females was even, and the cats were aged 1-3 years and weighed approximately 2.5-4 kg. The test animals received necessary daily vaccinations and deworming treatments, had free access to water, and their enclosures were cleaned and maintained. The pet rooms were kept clean. The "single-feeding bowl method" was used for the test, with a 7-day experimental period and a 4-day regular feeding period. During the experimental period, starting at 3:00 PM each day, 100g of the functional cat food from Example 9 was weighed for each test cat. After completion, the food bowl was placed in the corresponding test cat's room. The food bowl was removed at 3:00 PM the following afternoon, the remaining amount was weighed, and the test food was replenished to 100g. The food intake and fecal scores of the test cats were recorded during this period. The fecal score criteria are as follows: Figure 9 As shown, observe the pet's mental state and note any adverse reactions such as aversion, diarrhea, or vomiting. Take photos as needed.

[0233] Note: The average daily food intake of the test cats is measured in grams per day per cat (data compiled from the analysis of 60 cats fed daily cat food for 10 days). According to the fecal scoring standard, a fecal score of 1.6-2.8 (inclusive) is the standard fecal score range. A score below 1.6 indicates overall constipation, 1.6-1.75 indicates mild overall constipation, 2.8-3.5 (inclusive) indicates soft stools, and a score above 3.5 indicates diarrhea. A qualified test food must not only meet the fecal score requirement but also have a fecal pass rate of over 80%. The fecal pass rate refers to the proportion of all test cats whose fecal scores fall within the standard fecal score range. The vomiting rate of all test cats must not exceed 5%. All fecal and vomiting data refer to data collected during the regular feeding period.

[0234] Table 7 Research results of the cat food of this invention

[0235] Note: The data is the average of all animal data for a certain day or period of time.

[0236] During the trial period, the cats consumed 60.29g / day of the complete gut health cat food, had a fecal score of 2.23, a fecal pass rate of 100%, a vomiting rate of 0%, and were in good mental condition.

[0237] Example 5: Study on the effect of the cat food of the present invention on the intestinal health of cats

[0238] 1. Test Sample: Functional cat food from Example 9.

[0239] 2. Experimental Animals and Experimental Design

[0240] 2.1 Experimental Design

[0241] The experiment lasted 28 days. During the experiment, 20 healthy adult cats weighing 3.26±0.44kg and of random sex were selected and divided into two groups of 10 cats each. One group (experimental group) was fed functional cat food, and the other group (control group) was fed control cat food.

[0242] The cat food in this sample was purchased from Shandong Shuaike Pet Products Co., Ltd. Its ingredients include: fresh chicken, corn, rice, beef meal, fish meal, duck oil, brewer's yeast powder, chicken liver powder, beet pulp pellets, psyllium husk, kelp powder, and brewer's yeast cell walls. Additives include: natural steroids such as saponins, taurine, fructooligosaccharides, L-lysine, dl-α-tocopherol, vitamin A, vitamin D3, vitamin B1, vitamin B2, vitamin B6, vitamin B12, vitamin C, folic acid, niacinamide, D-calcium pantothenate, choline chloride, sodium chloride, antioxidants, calcium iodate, zinc glycine, iron glycine complex, copper glycine complex, and manganese methionine complex.

[0243] Each cat was housed individually in a pet cage (1.6 m × 0.7 m × 0.7 m). Necessary vaccinations and deworming were performed before the experiment. The litter box was changed daily, and the enclosures were cleaned and maintained to ensure cleanliness. During the experiment, each cat in the experimental group was fed 100g of cat food and 300mL of water every morning at 8:30 AM. The feeding status and mental state of all pets were observed during the experiment, and any adverse reactions such as aversion, diarrhea, or vomiting were noted.

[0244] 2.2 Growth performance determination

[0245] The cats were weighed on an empty stomach before the experiment and on day 28 of the experiment. The food intake and water consumption of the cats were recorded before each morning feeding. At the end of the experiment, the average daily food intake and average daily water consumption of all cats were calculated.

[0246] 2.3 Gut Health Assessment

[0247] At the end of the experiment, fresh feces were collected and frozen at -80°C in cryovials. On day 28, three cats from each group were collected, and the samples were sent to Shanghai Meiji Biotechnology Co., Ltd. for 16S rDNA metagenomic sequencing using high-throughput sequencing technology. To study the species composition of each sample, OTUs (Operational Taxonomic Units) were clustered based on the effective tags of all samples with 97% identity, and then the OTU sequences were annotated for species. Based on the OTU results obtained from the clustering and the research needs, the common and unique OTUs among different samples (groups) were analyzed and plotted as Venn graphs.

[0248] To observe the differences in species richness among groups, the top ten most abundant species were selected and their gut microbiota differences were compared and analyzed at the phylum and genus levels using a T-test. Species with significant differences in abundance changes between groups at the phylum and genus levels were specifically identified, and the enrichment of these differentially abundant species across different groups was determined to assess the significance of community structure differences between groups. LDA values ​​for species were calculated using LEfSe (LDA Effect Size) analysis, and differentially abundant species with LDA values ​​greater than 2 were selected. Bar charts and cladistic diagrams of LDA value distributions were then plotted to compare species differences at each level.

[0249] 3. Experimental Results

[0250] 3.1 The effect of functional cat food on the eating performance of cats

[0251] As shown in Table 8, when there was no significant difference in initial body weight, there were no significant differences in final body weight, daily food intake, and water consumption between the experimental group and the control group. P >0.05).

[0252] Table 8. Effects of Functional Cat Food on Feeding Characteristics of Cats

[0253] 3.2 Effects of functional cat food on the gut microbiota of pet cats

[0254] 3.3.1 Venn Diagram

[0255] Depend on Figure 10 It can be seen that the experimental group (A) and the control group (C) contain 34 and 31 OUTs respectively, with a total of 233 between the groups.

[0256] 3.3.2 Phylum-level species composition

[0257] As shown in Table 9, the top three most abundant species in the experimental group at the phylum level were: Bacillota , Bacteroidota , Actinomycetota These account for over 85% of the total species. The top three most abundant species in the control group were... Bacillota , Bacteroidota , Actinomycetota They account for over 96% of the total species, in the experimental group. Pseudomonadota (Proteobacteria) was significantly higher than the control group ( P <0.05), with no significant differences in gut microbiota among other pet cat species ( P >0.05).

[0258] Table 9. Species composition and relative abundance (%) of the feline gut microbiota.

[0259] 3.3.3 Genus-level species composition

[0260] As shown in Table 10, the experimental group Blautia (Broutella) Peptoclostridium (Clostridium genus) Gemmi Ger (a spore-forming fungus) had significantly lower levels than the control group ( P <0.05), there was no significant difference at the species level of the genus *Catella*. P >0.05).

[0261] Table 10. Genus-level species composition and relative abundance (%) of feline gut microbiota

[0262] 3.3.5 LEfSe Analysis

[0263] Depend on Figure 11 It can be seen that the feline intestinal experimental group g_ Pediococcus (Pediococcus), f_ Lactobacillaceae (Lactobacillus family), c_ Gammaproteobacteria (Gammaproteobacteria), p_ Pseudomonadota (Pseudomonas), g_ Anaerobiospirillum (Anaerobic Spirogyra), f_ Succinivibrionaceae (Acidovibrioceae), o_ Burkholderial (Burkholderiales), f_ Sutterellaceae Significant enrichment of *Sartreus* genus (family Sartreusaceae) and *g_Lachnospiraceae_UCG-010* (genus *Spirulina*). Control group c_ Clostridia (Clostridium), o_ Lachnospirales (Clostridiales), g_ Blautia (Broutella) f_ Peptostreptococcaceae (Peptostreptococcus), f_ Lachnospiraceae Significant enrichment of fungi such as (Trichophyceae family).

[0264] This study investigated the effects of functional cat food on the gut health of cats. After feeding cats with functional cat food for 28 days, the following conclusions were drawn: feeding functional cat food did not have an adverse effect on the cats' weight, food intake, or water intake; feeding functional cat food increased the number and abundance of beneficial bacteria in the cats' gut, enhanced immunity, prevented intestinal infections and diarrhea, and protected the cats' intestinal barrier.

[0265] Example 6: Test on the effect of the cat food of the present invention in improving feline diarrhea.

[0266] 1. Experimental Methods

[0267] Based on the literature, the feces of cats with diarrhea in this experiment were scored as follows: 1 point: feces are single spherical, hard, and dry; leave no trace when picked up; mild constipation. 2 points: feces are long and thin, flexible, not hard, and segmented; leave almost no trace when picked up; healthy feces. 3 points: feces are long and thin, smooth, and almost unsegmented; leave a slight trace when picked up; healthy feces. 4 points: feces are short and cylindrical, moist; leave a trace when picked up, sticky, but still retain a certain shape; sub-healthy feces. 5 points: feces are moist and pasty, with a certain shape; cannot be picked up; mild diarrhea. 6 points: fecal texture, no fixed shape; loose and pasty, with a mixture of solids and liquids; cannot be picked up; moderate diarrhea. 7 points: no fecal texture; watery stool, no solid part; cannot be picked up; severe diarrhea.

[0268] Six healthy cats and 30 cats with diarrhea were selected. Inclusion criteria for healthy cats: they had been dewormed and vaccinated according to standard procedures, had a fecal score of 2.5–3.0, and showed no significant abnormalities in physical examination and fecal examination. Exclusion criteria: cats that had used antibiotics or probiotics within one month prior to the experiment were not included. Inclusion criteria for cats with diarrhea: a fecal score greater than 3.0. Exclusion criteria: cats that had used antibiotics or probiotics within one month prior to the experiment were not included.

[0269] The cats with diarrhea were randomly divided into four groups: diarrhea group, Example 9 group, Comparative Example 9 group, Comparative Example 10 group, and Comparative Example 11 group, with 6 cats in each group.

[0270] The healthy group and the diarrhea group were fed regular cat food; Example 9 group was fed Example 9 cat food; Comparative Example 9 group was fed the product of Comparative Example 9; Comparative Example 10 group was fed the product of Comparative Example 10; Comparative Example 11 group was fed regular cat food and also received the product of Comparative Example 11 at a dose of 0.1 g / kg body weight. The daily feeding amount was the same for all groups of cats, and the feeding was continued for 7 days.

[0271] The regular cat food is purchased from Shandong Shuaike Pet Products Co., Ltd. Ingredients include: fresh chicken, corn, rice, beef meal, fish meal, duck fat, brewer's yeast powder, chicken liver powder, beet pulp pellets, psyllium husk, kelp powder, and brewer's yeast cell walls. Additives include: natural steroids such as saponins, taurine, fructooligosaccharides, L-lysine, dl-α-tocopherol, vitamin A, vitamin D3, vitamin B1, vitamin B2, vitamin B6, vitamin B12, vitamin C, folic acid, niacinamide, D-calcium pantothenate, choline chloride, sodium chloride, antioxidants, calcium iodate, zinc glycine, iron glycine complex, copper glycine complex, and manganese methionine complex.

[0272] 2. Detection indicators

[0273] Evaluation of the effectiveness of treatment for feline diarrhea

[0274] The effectiveness of treatment in domestic cats was evaluated by observing their clinical manifestations after treatment, such as their mental state, appetite, defecation pattern, frequency of defecation, and defecation time, as well as by using a diarrhea grading scale to assess the treatment effect.

[0275] Fresh fecal samples were collected from cats in each group on day 7 of drug administration for gut microbiota analysis. For Alpha diversity analysis, the richness and diversity of the microbial community in clinical samples were assessed primarily using diversity indices. Differences were represented by the ACE index after classification at the OUT level.

[0276] 3. Statistical Methods

[0277] Data processing was performed using GraphPad Prism 7.0 software, with the measurement data presented in... ±s indicates that the comparison between groups was performed using one-way ANOVA, and P<0.05 was considered statistically significant.

[0278] 4. Experimental Results

[0279] 4.1 Effect on feline diarrhea scores

[0280] Table 11 Comparison of diarrhea scores among different groups of cats

[0281] Note: Compared with the healthy group, * P < 0.05 ** P < 0.01; compared with the diarrhea group, & P < 0.05; && P < 0.01; compared with Example 9 group, # P < 0.05 ## P < 0.01.

[0282] The results are shown in Table 11. Compared with the healthy group, the diarrhea scores of the cats in the diarrhea group were significantly different.

[0283] Compared to the diarrhea group, the diarrhea score of cats in Example 1 was significantly reduced. Compared to Comparative Examples 9-11, Example 9 showed better results in improving feline diarrhea.

[0284] 4.2 Alpha Diversity ACE Index Analysis

[0285] Table 12 Analysis of Alpha Diversity and ACE Index of Gut Microbiota in Cats of Each Group

[0286] Note: Compared with the healthy group, * P < 0.05 * P < 0.01; compared with the diarrhea group,& P < 0.05; && P < 0.01; compared with Example 9 group, # P < 0.05 ## P < 0.01.

[0287] The results are shown in Table 12. Compared with the healthy group, the ACE index of cats in the diarrhea group was significantly different.

[0288] Compared with the diarrhea group, the ACE index of cats in Example 1 group was significantly increased. Compared with Comparative Examples 9-11, the ACE index of Example 9 group was significantly different.

[0289] The experimental results show that the cat food of the present invention improves the symptoms of diarrhea in cats, regulates the intestinal flora structure of cats, and restores the composition and abundance of the flora to the level of normal healthy cats, providing a new solution for the treatment of cat diarrhea.

[0290] The only difference between Comparative Example 9 and Example 9 is the change in the composition of probiotics and prebiotics. Xylooligosaccharides were used instead of chicory root powder, and Lactobacillus rhamnosus was used instead of Bacillus subtilis. The product of Comparative Example 9 was less effective in improving feline diarrhea than the cat food of Example 9. The only difference between Comparative Example 10 and Example 9 is the change in the composition of probiotics and prebiotics. Maltodextrin was used instead of fructooligosaccharides, and Lactobacillus acidophilus was used instead of Lactobacillus plantarum. The product of Comparative Example 10 was less effective in improving feline diarrhea than the cat food of Example 9. The results of Comparative Examples 9 and 10 show that, under the same dosage of functional pet food, changing the raw materials of the functional pet food reduces the effect on improving feline intestinal health to varying degrees. The raw materials of the functional pet food of this invention have a synergistic effect. Compared with the formulation of Comparative Example 11, the functional pet food of this invention has a better effect on improving feline intestinal health and has significant technical advantages.

[0291] The above descriptions are merely embodiments of the present invention, and common knowledge such as specific technical solutions and / or characteristics are not described in detail here. It should be noted that those skilled in the art can make various modifications and improvements without departing from the technical solutions of the present invention, and these should also be considered within the scope of protection of the present invention. These modifications and improvements will not affect the effectiveness of the implementation of the present invention or the practicality of the patent. The scope of protection claimed in this application should be determined by the content of its claims, and the specific embodiments described in the specification can be used to interpret the content of the claims.

Claims

1. A functional pet food for regulating intestinal health, characterized in that, The functional pet food contains the following components in parts by weight: sweet potato powder 20-30 parts, chicken powder 15-30 parts, enzymatic chicken 5-15 parts, chicken bone powder 2-8 parts, chicken oil 5-15 parts, starch 5-13 parts, chicken liver powder 0.5-1.5 parts, beet pulp 0.5-3.5 parts, fish oil 0.2-0.8 parts, flaxseed oil 0.1-0.2 parts, cellulose 0.5-1.5 parts, fruit and vegetable powder 2-8 parts, vitamin and mineral premix 1-3 parts, yeast hydrolysate 0.5-1 part, lily powder 0.05-0.15 parts, fruit oligosaccharide 0.01-0.1 part, chicory root powder 0.05-0.15 parts, Chinese herbal medicine premix 0.2-3 parts, and compound probiotics 0.01-0.03 parts.

2. The functional pet food for regulating intestinal health according to claim 1, characterized in that, The Chinese herbal medicine premix is composed of ginseng 5-15 parts, white atractylodes 5-15 parts, licorice 5-15 parts, alisma 5-15 parts, hawthorn 5-15 parts, malt 5-15 parts, lotus seed 15-25 parts, Chinese yam 15-25 parts, and poria 15-25 parts.

3. The functional pet food for regulating intestinal health according to claim 1, characterized in that, The compound probiotics are composed of Bacillus subtilis, Enterococcus faecium, Lactobacillus plantarum, Bacillus coagulans, and Lactobacillus reuteri in a mass ratio of 1-2:0.5-1:2-4:0.5-1:2-3.

4. The functional pet food for regulating intestinal health according to claim 1, characterized by, The starch is one or more of corn starch, cassava starch, or potato powder.

5. The functional pet food for regulating intestinal health according to claim 1, characterized by, The fruits and vegetables are one or more of carrot powder, pumpkin powder, apple powder, pear powder, or alfalfa.

6. The functional pet food for regulating intestinal health according to claim 1, wherein, The grains are one or more of rice, corn, wheat, millet, oat, or quinoa.

7. The functional pet food for regulating intestinal health according to claim 1, characterized by, The functional pet food contains the following components in parts by weight: sweet potato powder 25 parts, chicken powder 22.5 parts, enzymatic chicken 10 parts, chicken bone powder 5 parts, chicken oil 10 parts, starch 9 parts, chicken liver powder 1 part, beet pulp 2 parts, fish oil 0.5 parts, flaxseed oil 0.15 parts, cellulose 1 part, fruit and vegetable powder 5 parts, vitamin and mineral premix 2 parts, yeast hydrolysate 0.75 parts, lily powder 0.1 parts, fruit oligosaccharide 0.05 parts, chicory root powder 0.1 parts, Chinese herbal medicine premix 1.6 parts, and compound probiotics 0.02 parts. The Chinese herbal medicine premix is composed of ginseng 10 parts, white atractylodes 10 parts, licorice 10 parts, alisma 10 parts, hawthorn 10 parts, malt 10 parts, lotus seed 20 parts, Chinese yam 20 parts, and poria 20 parts. The compound probiotics are composed of Bacillus subtilis, Enterococcus faecium, Lactobacillus plantarum, Bacillus coagulans, and Lactobacillus reuteri in a mass ratio of 2:1:3:1:

2.

8. The functional pet food for regulating intestinal health according to claim 1, characterized by, The functional pet food contains the following components in parts by weight: sweet potato powder 20 parts, chicken powder 30 parts, enzymatic chicken 5 parts, chicken bone powder 2 parts, chicken oil 15 parts, starch 13 parts, chicken liver powder 0.5 parts, beet pulp 3.5 parts, fish oil 0.2 parts, flaxseed oil 0.2 parts, cellulose 0.5 parts, fruit and vegetable powder 8 parts, vitamin and mineral premix 1 part, yeast hydrolysate 1 part, lily powder 0.05 parts, fruit oligosaccharide 0.1 part, chicory root powder 0.05 parts, Chinese herbal medicine premix 3 parts, and compound probiotics 0.01 parts. The Chinese herbal medicine premix is composed of 15 parts of ginseng, 15 parts of white atractylodes, 5 parts of licorice, 5 parts of alisma, 15 parts of hawthorn, 15 parts of malt, 15 parts of lotus seed, 15 parts of Chinese yam, and 25 parts of poria. The complex probiotics are composed of Bacillus subtilis, Enterococcus faecium, Lactobacillus plantarum, Bacillus coagulans and Lactobacillus reuteri with a mass ratio of 2:1:3:1:

2.

9. The functional pet food for regulating intestinal health according to claim 1, wherein, The functional pet food contains the following components in parts by weight: 30 parts of sweet potato powder, 15 parts of chicken powder, 15 parts of enzymatic chicken, 8 parts of chicken bone meal, 5 parts of chicken oil, 5 parts of starch, 1.5 parts of chicken liver powder, 0.5 parts of beet pulp, 0.8 parts of fish oil, 0.1 part of flaxseed oil, 1.5 parts of cellulose, 2 parts of fruit and vegetable powder, 3 parts of vitamin and mineral premix, 0.5 parts of yeast hydrolysate, 0.15 parts of yucca powder, 0.01 parts of fructo-oligosaccharide, 0.05 parts of chicory root powder, 0.2 parts of Chinese herbal medicine premix, and 0.03 parts of complex probiotics. The Chinese herbal medicine premix is composed of 5 parts of ginseng, 5 parts of white atractylodes, 15 parts of licorice, 15 parts of alisma, 5 parts of hawthorn, 5 parts of malt, 25 parts of lotus seed, 25 parts of Chinese yam, and 15 parts of poria. The complex probiotics are composed of Bacillus subtilis, Enterococcus faecium, Lactobacillus plantarum, Bacillus coagulans and Lactobacillus reuteri with a mass ratio of 1:0.5:2:0.5:

3.

10. The functional pet food for regulating intestinal health according to claim 1, characterized by, The preparation method comprises the following steps: Step (1) After batching, the raw materials are crushed and passed through a 40-mesh sieve, and then the sweet potato powder, chicken powder, enzymatic chicken, chicken bone meal, starch, beet pulp, cellulose, fruit and vegetable powder, yeast hydrolysate, yucca powder, fructo-oligosaccharide, chicory root powder, Chinese herbal medicine premix, vitamin and mineral premix are added and mixed, and then the mixture is fed into a conditioner; and then the mixture is subjected to the processes of puffing, drying and spraying in sequence, and the chicken oil, fish oil, flaxseed oil, complex probiotic powder and chicken liver are added during the spraying process to obtain dog food granules; Step (2) After cooling, the dog food granules are sieved and packaged to obtain the product.

Images