Hair loss preventing and fixing agent containing recombinant XVII type collagen Recomtein PHD and water-soluble fullerene and preparation method of hair loss preventing and fixing agent

Abstract

The invention discloses an anti-hair loss and hair consolidation agent containing recombinant XVII type collagen Recomtein PHD and water-soluble fullerene and a preparation method of the anti-hair loss and hair consolidation agent. The composition is composed of freeze-dried powder and a solvent, the freeze-dried powder is XVII type collagen, and the solvent comprises water-soluble fullerene, water, butanediol, p-hydroxyacetophenone and 1, 2-hexanediol. According to the composition, through a double-effect synergistic mechanism of'stem cell microenvironment repair-free radical targeted removal ', the anti-hair loss effect is remarkably improved, and the problems that active ingredients of a traditional anti-hair loss product are prone to inactivation, the delivery efficiency is low, and sensitive scalp tolerance is poor are solved. The freeze-dried powder dosage form ensures the stability of active ingredients, and fullerene is fresh and cool in texture after being redissolved as a solvent and is easy to permeate hair follicle targets. The anti-hair-loss and hair-strengthening composition provided by the invention has the effects of efficiently resisting oxidation, promoting hair follicle regeneration and delaying scalp aging, and is suitable for the field of anti-hair-loss scalp care.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic raw materials, specifically relating to a hair loss prevention and hair strengthening agent containing recombinant type XVII collagen RecomteinPHD and water-soluble fullerene, and its preparation method. Background Technology

[0002] The essence of hair loss lies in the progressive decline of hair follicle regeneration function, and its core pathological mechanism is driven by two axes: On the one hand, hair follicle stem cells (HFSCs) rely on XVII collagen in the hemidesmosomes of the basement membrane to maintain anchoring stability. With age and increased oxidative stress (UV / stress-induced), XVII collagen degradation leads to HFSC "anorexia and apoptosis." After stem cells are removed from the microenvironment, they differentiate abnormally or die, while inhibiting the Wnt / β-catenin pathway, which shortens the hair follicle growth phase. On the other hand, the burst of reactive oxygen species (ROS) forms a vicious cycle: free radicals such as ·OH continuously attack the mitochondrial DNA of HFSCs, leading to energy depletion, damaging collagen fiber structure, and activating inflammatory factors such as TNF-α, accelerating the miniaturization process of hair follicles.

[0003] Traditional anti-hair loss products suffer from limited effectiveness, inactivation of active ingredients, and low delivery efficiency. Liquid anti-hair loss serums have a high sensitization rate due to preservatives such as phenoxyethanol, and recombinant protein active ingredients are easily inactivated, leading users to question the long-lasting efficacy of these products. Furthermore, traditional formulations struggle to balance the stability of active ingredients with scalp penetration. Summary of the Invention

[0004] The present invention aims to provide a hair loss prevention and strengthening agent containing recombinant type XVII collagen Recomtein PHD and water-soluble fullerene, and its preparation method, to protect the user's hair and slow down and improve hair loss.

[0005] To achieve the above-mentioned technical objectives, the present invention provides the following technical solution:

[0006] A hair loss prevention and hair strengthening agent containing type XVII collagen (Collagen XVII, COL17A1, BP180) and water-soluble fullerene (Fullerene C60), comprising a lyophilized powder with type XVII collagen anti-hair loss and hair strengthening effect and a hair loss prevention and hair strengthening solvent containing water-soluble fullerene, wherein the lyophilized powder comprises the following components in volume percentages:

[0007] Recomtein ® PHD Fragrance: 100% (10,000-20,000 IU / bottle);

[0008] The solvent is composed of the following components by volume percentage:

[0009] Starfact® Hydro-C60 water-soluble fullerene: 10%

[0010] Water: 85.2%;

[0011] Butylene glycol: 4%;

[0012] p-Hydroxyacetophenone: 0.3%;

[0013] 1,2-Hexanediol: 0.5%;

[0014] The hair loss prevention and hair strengthening agent containing recombinant type XVII collagen Recomtein PHD and water-soluble fullerene described in this invention has a collagen activity of 10,000-20,000 IU in the freeze-dried powder with type XVII collagen anti-hair loss and hair strengthening effect.

[0015] The present invention describes a hair loss prevention and hair strengthening agent containing recombinant type XVII collagen Recomtein PHD and water-soluble fullerene. In the hair loss prevention and hair strengthening solvent containing water-soluble fullerene, the fullerene is hydrophilically modified to balance its effectiveness and stability.

[0016] The composition of this invention significantly enhances the anti-hair loss effect through a dual-effect synergistic mechanism of "stem cell microenvironment repair and free radical targeted scavenging," solving problems such as easy inactivation of active ingredients, low delivery efficiency, and poor tolerance of sensitive scalps in traditional anti-hair loss products. The freeze-dried powder formulation ensures the stability of the active ingredients, and the fullerene, as a solvent, provides a refreshing texture after reconstitution, facilitating easy penetration into hair follicle targets. The anti-hair loss and hair-strengthening composition provided by this invention has highly effective antioxidant properties, promotes hair follicle regeneration, and delays scalp aging, making it suitable for the field of hair loss prevention and scalp care.

[0017] A method for preparing a hair loss prevention and hair strengthening agent containing recombinant type XVII collagen (Recomtein PHD) and water-soluble fullerene, wherein the method is used to prepare a hair conditioner having the hair loss prevention and hair strengthening effect of type XVII collagen and water-soluble fullerene as described in any one of claims 1-4; the steps of the method are as follows:

[0018] S1: Clean and disinfect all utensils and containers, and prepare a hot water bath for heating the water phase;

[0019] S2: Mix the components (butanediol and p-hydroxyacetophenone) in the aqueous phase in a hot water bath, place the hot water bath in the hot water bath and heat it at 60-70℃, stirring constantly until all components are dissolved evenly.

[0020] S3: After cooling to room temperature, add the other components of the aqueous phase (water, water-soluble fullerene, and 1,2-hexanediol) to the aqueous phase pot and keep stirring until all components are completely mixed and homogeneous to obtain a solvent with water-soluble fullerene anti-hair loss and hair-strengthening effects. Put the solvent with water-soluble fullerene anti-hair loss and hair-strengthening effects into a clean container and seal it for storage.

[0021] The method of using the hair loss prevention and hair strengthening composition and the hair conditioner containing type XVII collagen and water-soluble fullerene for hair loss prevention and hair strengthening includes the following steps:

[0022] (1) After opening the packaging of the solvent with water-soluble fullerene anti-hair loss and hair strengthening effect, add it to the freeze-dried powder with XVII type collagen anti-hair loss and hair strengthening effect, cover the inner stopper and shake up and down until the freeze-dried powder is completely dissolved in the solvent to form an essence.

[0023] (2) After washing your hair, wipe the area to be treated with 75% alcohol wipes to disinfect it. Apply some of the above-mentioned essence to the scalp area that needs to be treated and spread it evenly.

[0024] (3) Use disposable microneedles to roll in a uniform speed and sequence 4-5 times on the surface of the skin to be treated in the longitudinal, transverse and 45-degree directions.

[0025] (4) Apply the remaining anti-hair loss essence to the areas that need care after the roller has been rolled.

[0026] In this invention, a hair loss prevention and hair strengthening agent containing recombinant type XVII collagen Recomtein PHD and water-soluble fullerene is used to prevent hair loss, promote hair follicle regeneration, and delay scalp aging.

[0027] This invention discloses an anti-hair loss and hair-strengthening hair conditioner containing type XVII collagen and water-soluble fullerene (Hydro-C60), as well as its preparation and application methods. The hair conditioner consists of two parts: a freeze-dried powder containing highly active type XVII collagen, and a solvent containing water-soluble fullerene and other auxiliary ingredients. The two are immediately reconstituted before use to form an anti-hair loss essence with synergistic effects. Type XVII collagen is a key structural protein maintaining the connection between hair follicle stem cells (HFSCs) and the basement membrane, and can delay hair follicle aging by repairing hemidesmosome structure and maintaining the stability of the stem cell microenvironment. Water-soluble fullerene possesses extremely strong antioxidant capabilities, can target and scavenge harmful free radicals such as ·OH, reduce oxidative stress damage to hair follicles, and acts as a functional solvent to enhance the stability and penetration efficiency of active ingredients. The present invention also provides a preparation process for the composition, including low-temperature vacuum drying of the lyophilized powder and a mild compounding process of the solvent, to ensure the high efficiency, safety and stability of the final product.

[0028] Compared with existing technologies, the hair conditioner preparation method of the present invention has the following superior effects:

[0029] 1. The hair loss prevention and hair strengthening agent preparation method with type XVII collagen and water-soluble fullerene provided by the present invention adopts the form of separate packaging of freeze-dried powder and solvent and on-site reconstitution before use, which avoids the degradation and inactivation of active ingredients during storage and ensures that the product maintains the best active state when used. Compared with the traditional liquid storage method, it has a significant stability advantage.

[0030] 2. In the preparation process provided by the present invention, the freeze-dried powder is treated with low-temperature vacuum sublimation technology to treat type XVII collagen, which effectively maintains the biological activity and structural integrity of the protein and avoids the damage of active ingredients to high-temperature treatment. Compared with traditional heat-sensitive ingredient treatment processes, it is gentler and safer.

[0031] 3. The present invention adopts a stepwise preparation process, in which the aqueous components are first heated and dissolved and then cooled and mixed. The process conditions are mild and controllable, the operation is simple, and it is easy to carry out large-scale industrial production. Compared with the complex one-pot preparation process, it has higher operability and reproducibility.

[0032] 4. In the anti-hair loss and hair strengthening agent prepared by the present invention, the water-soluble fullerene not only plays a strong antioxidant role as a functional ingredient, but also enhances the solubility and permeability of other active ingredients as a solvent, realizing the dual action mechanism of "solvent is efficacy". Compared with traditional single carrier solvents, it has better comprehensive performance.

[0033] 5. In the preparation method provided by the present invention, the freeze-dried powder preparation process adopts sterile filtration and freeze-drying technology, which naturally inhibits the growth of microorganisms and ensures product safety without the need to add chemical preservatives. Compared with traditional liquid formulas containing preservatives, it is milder and safer, and is especially suitable for sensitive scalps.

[0034] 6. Since both type XVII collagen and water-soluble fullerene have the effect of preventing hair loss and strengthening hair, the hair conditioner prepared by the preparation method provided by this invention can achieve a synergistic effect of dual anti-hair loss mechanism, and has excellent anti-hair loss and hair follicle regeneration effects, which are more significant than single-ingredient products.

[0035] 7. The hair loss prevention and hair strengthening agent prepared by this invention adopts a freeze-dried powder and solvent separate packaging form, which is mixed immediately before use. This not only ensures the freshness of the active ingredients, but also enhances the user's technological experience and usage compliance. Compared with traditional premixed products, it has better market acceptance and application prospects. Attached Figure Description

[0036] Figure 1 This is a graph showing the change in the average amount of hair loss over time.

[0037] Figure 2 This is a graph showing the rate of change in the amount of hair loss.

[0038] Figure 3 This is a graph showing the change of the mean local hair density over time.

[0039] Figure 4 This is a graph showing the rate of change in local hair density.

[0040] Figure 5 Comparison of localized hair density increases for volunteer a (0W, 4W, 8W, 12W, 50x magnification).

[0041] Figure 6 Comparison images showing reduction in scalp redness in volunteer B (0W, 4W, 8W, 12W, 50x magnification);

[0042] Figure 7 Comparison images showing reduction in scalp redness in volunteer C (0W, 4W, 8W, 12W, 200x magnification);

[0043] Figure 8 Comparison of volunteer d's hair diameter as it thickens (0W, 12W, 200x magnification);

[0044] Figure 9 Comparison of volunteer e's overall scalp (0W, 12W);

[0045] Figure 10 Comparison of volunteer f's overall scalp (0W, 12W). Detailed Implementation

[0046] Based on the above-disclosed content of this invention, and in accordance with common technical knowledge and conventional methods in the field, various other modifications, substitutions, or alterations can be made without departing from the basic technical ideas disclosed in this invention.

[0047] Example 1

[0048] This embodiment evaluates the effectiveness of the hair loss prevention essence set (containing freeze-dried powder and solvent) in reducing the amount of hair loss, increasing local hair density, and improving scalp health through human efficacy testing.

[0049] (1) Test sample

[0050] The anti-hair loss essence set includes:

[0051] 1. Lyophilized powder: The main active ingredient is Recomtein. ® PHD Fuyuxin (purchased from Guangzhou Jikemeichuang Life Co., Ltd.) (10,000-20,000 IU / bottle), also known as type XVII collagen.

[0052] 2. Solvent: The main active ingredient is 10% by mass. (Starfact) ® Hydro-C60 water-soluble fullerene (purchased from Zhejiang Chenhai Life Science Co., Ltd.), Starfact ® Hydro-C60 water-soluble fullerenes are prepared by mechanically dispersing fullerenes in polymer materials. This method maintains the fullerene structure and efficacy while preserving the fullerene's structure. The final material contains no organic reagent residues, which is beneficial for industrial applications. It contains 85.2% water, 4% butanediol, 0.3% p-hydroxyacetophenone, and 0.5% 1,2-hexanediol.

[0053] 3. Complementary anti-hair loss scalp essence (cream): Contains 1% GROWTH Oléoactif ® , 2% CAFFENERGY50C, 10% Supferm ® CORO (LAB) Skin Revitalizing Coffee, 2% AKOSKY AZUKI AS Double Seed Hair Strengthening Formula, 3.4% Starfact ® Hydro-C60 contains water-soluble fullerenes, 3% SSG-210SP, 1% KSG-15AP, 4% KF-96A-6T, 0.15% KF-6038, 5% KF-995, 0.2% KSP-101, 46.85% water, 10% butanediol, 10% methyl methacrylate, 0.5% NaCl, 0.4% acetone, and 0.5% 1,2-hexanediol.

[0054] substance Purchase location <![CDATA[GROWTH Oléoactif ® Ingredients: Sunflower (HELIANTHUS ANNUUS) seed oil 66-70%, polyglycerol-3 diisostearate 10-14%, safflower (CARTHA MUSTINCTORIUS) flower extract 8-12%, hibiscus (HIBISCUS SABDARIFFA) flower extract 8-12%, tocopherol (vitamin E) 0.1-0.3% Hallstar Beauty andPersonal Care SolutionsCompany CAFFENERGY 50C: 50% Caffeine, 50% Cyclodextrin AXIALYS INNOVATIONS <![CDATA[Supferm ® CORO (LAB) Skin Revitalizing Coffee: Lactobacillus ferment 89.5-93.5%, Coffea rubra seed extract 1.0-3.0%, 1,3-propanediol 5.0-10.0%, 1,2-hexanediol 0.5-2.0%, p-hydroxyacetophenone 0.3-1.0% Zhejiang Starea LifeSciences Co., Ltd. AKOSKY AZUKI AS Double Seed Hair Strengthening Formula: Glycerin 90-98%, Water 2-3%, Green Bean (VIGNARADIATA) Seed Extract 0.1-0.3%, Red Clover (TRIFOLIUM PRATENSE) Extract 0.2-0.4%, Sodium Benzoate 0.6%, Potassium Sorbate 0.6%, Lactic Acid 0-0.5% Akott Evolution Srl <![CDATA[Starfact ® Hydro-C60 Water-Soluble Fullerene: Fullerene 0.030-0.045%, Butylene Glycol 75%, Polyvinylpyrrolidone 10%, Water 14.955-14.970% Zhejiang Starea LifeSciences Co., Ltd. SSG-210SP: Polydimethylsiloxane 75.28%, Polydimethylsiloxane PEG-10 / 15 crosspolymer 24.00%, Dipropylene glycol 0.50%, Sodium citrate 0.20%, Tocopherol (Vitamin E) 0.02% SHIN-ETSU SILICONE INTERNATIONAL TRADING(SHANGHAI) CO.,LTD. KSG-15AP: 95% cyclopentamethoxysiloxane, 5% polydimethylsiloxane / vinyl polydimethylsiloxane crosspolymer SHIN-ETSU SILICONE INTERNATIONAL TRADING(SHANGHAI) CO.,LTD. KF-96A-6T: 100% Polydimethylsiloxane SHIN-ETSU SILICONE INTERNATIONAL TRADING(SHANGHAI) CO.,LTD. KF-6038: Lauryl PEG-9 polydimethylsiloxane ethyl polydimethylsiloxane 99.98%, tocopherol (vitamin E) 0.02% SHIN-ETSU SILICONE INTERNATIONAL TRADING(SHANGHAI) CO.,LTD. KF-995: 100% Cyclopentasiloxane SHIN-ETSU SILICONE INTERNATIONAL TRADING(SHANGHAI) CO.,LTD. KSP-101: 100% Vinyl polydimethylsiloxane / polymethylsiloxane silsesquioxane crosspolymer SHIN-ETSU SILICONE INTERNATIONAL TRADING(SHANGHAI) CO.,LTD. Fresh ketone: 100% p-hydroxyacetophenone Ashland Global Holdings Inc.

[0055] The preparation method of the solvent for water-soluble fullerene with anti-hair loss and hair-strengthening effects is as follows:

[0056] S1: Clean and disinfect all utensils and containers, and prepare a hot water bath for heating the water phase;

[0057] S2: Mix the components (butanediol and p-hydroxyacetophenone) in the aqueous phase in a hot water bath, place the hot water bath in the hot water bath and heat it at 60°C, stirring constantly until all components are dissolved and homogeneous.

[0058] S3: After cooling to room temperature, remove other components from the aqueous phase (water, Starfact) ® Hydro-C60 (water-soluble fullerene and 1,2-hexanediol) is added to an aqueous phase pot and stirred until all ingredients are completely mixed to obtain a solvent with water-soluble fullerene anti-hair loss and hair-strengthening effects. The solvent with water-soluble fullerene anti-hair loss and hair-strengthening effects is then placed into a clean container and sealed for storage.

[0059] The method for preparing freeze-dried powder is as follows:

[0060] (1) Ingredients: Pre-treated Recommendin ® PHD Fuyuxin XVII Collagen Solution is sterilized by microporous membrane filtration.

[0061] (2) Filling: Dispense into vials and place in a freeze dryer;

[0062] (3) Pre-freezing: Turn on the freeze dryer and maintain the temperature at 4℃±1℃ for 3 hours; cool down to below -20℃ and maintain the freeze drying chamber temperature at -20±2℃ for 6 hours to pre-freeze the product.

[0063] (4) First sublimation: Start evacuation. When the vacuum level reaches below 10 Pa, start heating. Adjust the temperature of the freeze-drying chamber to slowly rise to 0.0℃, with a temperature increase of no more than 1.0℃ per hour. Maintain the vacuum condition for 27 hours.

[0064] (5) Secondary sublimation: Continue to adjust the temperature of the freeze-drying chamber, raising it to 30-35℃ at a rate of 3-10℃ / h. Keep warm for 3-5 hours.

[0065] (6) Discharge: Introduce nitrogen, helium or argon, adjust the freeze-drying chamber to normal pressure and then press the inner plug, remove the gadolinium cap, and you will get freeze-dried powder with the anti-hair loss and hair-strengthening effect of type XVII collagen.

[0066] The freeze-dried powder and the water-soluble fullerene solvent, which has the effect of preventing hair loss and strengthening hair, are immediately reconstituted before use (the mass ratio of the two is 0.31:5) to form an anti-hair loss essence with synergistic effect.

[0067] The preparation method of the scalp anti-hair loss essence (cream) is as follows:

[0068] (1) Combine SSG-210SP, KSG-15AP, KF-96A-6T, KF-6038, KF-995, GROWTH Oléoactif ® 1. Homogenize KSP-101 for 3-5 min (rpm: 4000 rpm) until uniform and without obvious particles, then stir evenly to form phase A material. (2) Dissolve Xinxian ketone and butylene glycol in a 60-70℃ water bath until transparent and without particles;

[0069] (3) Add water, methyl alcohol, NaCl, 1,2-hexanediol, and CAFFENERGY 50C.

[0070] Vitality Coffee 50C, Supferm ® LAB(CORO)、AKOSKY AZUKI AS、Starfact ®Hydro-C60 water-soluble fullerenes were stirred until dissolved and free of particles.

[0071] (4) Mix the solutions from steps (2) and (3) to form phase B material.

[0072] (5) Under stirring conditions, slowly add B to phase A. After B is added, stir for 5-10 minutes (5-700 rpm), then homogenize at low speed (2-3000 rpm) for about 2 minutes until the paste is somewhat thick, and then discharge.

[0073] (2) Test method

[0074] ① Subject screening: 7 valid subjects, aged 18-60 years, with symptoms of hair loss and mild thinning, who had not dyed or permed their hair in the past month, and whose scalp was free of damage or disease.

[0075] ②Test environment: Temperature 20℃~26℃, humidity 40%~60%.

[0076] ③ Product Usage Instructions: Subjects should mix the solvent and freeze-dried powder to form a serum. Apply the serum evenly to a semi-dry scalp after washing, and use a heated shower cap for 20 minutes to promote absorption. On days when not using the freeze-dried serum, apply the accompanying anti-hair loss serum to the test area morning and evening. Wash hair every 2-3 days, using the freeze-dried powder set once. Alternate between the anti-hair loss serum and the freeze-dried powder set; otherwise, apply the accompanying anti-hair loss serum to the test area morning and evening.

[0077] (3) Evaluation indicators and time points

[0078] ① Number of hairs lost: Collect and count the hairs lost using the standard 60-comb method (30 times on each side).

[0079] ②Local hair density: Using the CBS-1717 hair follicle auxiliary analysis system, images were acquired and analyzed in a 1.5cm × 1.5cm area marked on the top of the head, slightly towards the temporal side.

[0080] ③ Follow-up visits and data collection: Follow-up visits were conducted before product use (0 weeks, 0W), 4 weeks after use (4W), 8 weeks after use (8W), and 12 weeks after use (12W), and the above indicators were measured repeatedly.

[0081] Efficacy Test Results Analysis

[0082] Test data are presented as mean values, and statistical methods were used for significance analysis (P < 0.05 was considered significant).

[0083] ① Changes in the number of hairs lost: As shown in Table 1 (average data of the number of hairs lost), the initial value (0W) was 22.60 hairs. After using the product, the number of hairs lost decreased significantly, dropping to 11.60 hairs, 8.80 hairs, and 9.20 hairs at 4W, 8W, and 12W, respectively. Figure 1 The graph showing the change in the average amount of hair loss over time visually illustrates this downward trend. Figure 2 As shown in the graph (hair loss rate change), compared with the initial value, the reduction rates of hair loss at 4W, 8W, and 12W reached 48.67%, 61.06%, and 59.29%, respectively. Statistical analysis showed significant differences at each time point compared with the initial value (4W: P=0.009; 8W: P=0.027; 12W: P=0.037), indicating that the sample had a significant effect on reducing the amount of hair loss.

[0084] ② Changes in local hair density: As shown in Table 2 (average local hair density data), the initial local hair density (0W) was 80.33 hairs / cm². After using the product, the density showed a continuous upward trend, increasing to 80.90 hairs / cm², 81.74 hairs / cm², and 82.99 hairs / cm² for 4W, 8W, and 12W, respectively. Figure 3 The graph showing the change in mean local hair density over time illustrates the density growth curve. (For example...) Figure 4 As shown in the local hair density change rate graph, compared with the initial value, the density increase rates at 4W, 8W, and 12W were 0.71%, 1.75%, and 3.31%, respectively. Although the changes at 4W and 8W did not reach the statistical significance level, at 12W, a statistically significant difference was observed compared with the initial value (P=0.041), indicating that the sample had a local effect of increasing hair density.

[0085] ③ Visual case improvement demonstration:

[0086] Increased hair density: such as Figure 5 As shown, during 12 weeks of product use, volunteer a showed an increase in localized hair density when the image was magnified 50 times.

[0087] Improved scalp health: such as Figure 6 and Figure 7 As shown, the scalp images of volunteers b and c (magnified by 50x and 200x respectively) show that the redness of the scalp improved with product use.

[0088] Hair shaft diameter increases: such as Figure 8 As shown, after 12 weeks of using the product, a comparison of images magnified 200 times revealed that the diameter of volunteer d's hair shaft had increased.

[0089] Overall appearance improvements: such as Figure 9 and Figure 10As shown in the comparison of overall scalp photos of volunteers e and f, scalp coverage improved after 12 weeks of use.

[0090] Table 1: Average Data on the Amount of Hair Loss

[0091] Time Group 0W 4W 4W change rate (%) 8W 8W Change Rate (%) 12W 12-week change rate (%) Anti-hair loss essence set 22.60 11.60 -48.67% 8.80 -61.06% 9.20 -59.29% Before and after comparison results (P-value) —— 0.009 —— 0.027 —— 0.037 —— Significance —— * —— * —— * ——

[0092] Table 2: Data on mean local hair density

[0093] Time Group 0W 4W 4W change rate (%) 8W 8W Change Rate (%) 12W 12-week change rate (%) Anti-hair loss essence set 80.33 80.90 0.71% 81.74 1.75% 82.99 3.31% Before and after comparison results (P-value) —— 0.416 —— 0.086 —— 0.041 —— Significance —— NS —— NS —— * ——

[0094] The above embodiments of the present invention are merely examples for clearly illustrating the present invention and are not intended to limit the implementation of the present invention. Those skilled in the art will recognize that other variations or modifications can be made based on the above description. It is neither necessary nor possible to exhaustively describe all possible implementations here. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention should be included within the scope of protection of the claims of the present invention.

Claims

1. A hair loss prevention and hair strengthening agent containing recombinant type XVII collagen (Recomtein PHD) and water-soluble fullerene, characterized in that, The composition comprises a freeze-dried powder with type XVII collagen anti-hair loss and hair-strengthening effects and a hair loss and hair-strengthening solvent containing water-soluble fullerene; the freeze-dried powder consists of the following components by volume percentage: Recomtein ® PHD (Fragrant): 100%; The hair loss prevention and hair strengthening solvent is composed of the following components by volume percentage: Water-soluble fullerenes: 5-15%; Water: 80-90%; Butylene glycol: 3-5%; p-Hydroxyacetophenone: 0.2-0.4%; 1,2-Hexanediol: 0.3-0.6%.

2. The hair loss prevention and hair strengthening agent containing recombinant type XVII collagen (Recomtein PHD) and water-soluble fullerene according to claim 1, characterized in that, The freeze-dried powder with type XVII collagen anti-hair loss and hair-strengthening effects contains collagen with an activity of 10,000-20,000 IU / bottle.

3. The hair loss prevention and hair strengthening agent containing recombinant type XVII collagen (Recomtein PHD) and water-soluble fullerene according to claim 1, characterized in that, The water-soluble fullerene is Starfact ® Hydro-C60 water-soluble fullerene.

4. The hair loss prevention and hair strengthening agent containing recombinant type XVII collagen (Recomtein PHD) and water-soluble fullerene according to claim 1, characterized in that, The mass ratio of the freeze-dried powder to the anti-hair loss and hair-strengthening solvent is 0.31:

5.

5. The hair loss prevention and hair strengthening agent containing recombinant type XVII collagen (Recomtein PHD) and water-soluble fullerene according to claim 1, characterized in that, It also includes a scalp anti-hair loss cream, which contains 1% GROWTH Oléoactif ® , 2% CAFFENERGY 50C, 10% Supferm ® CORO (LAB) Skin Revitalizing Coffee, 2% AKOSKYAZUKI AS Double Seed Hair Strengthening Formula, 3.4% Starfact ® Hydro-C60 contains water-soluble fullerenes, 3% SSG-210SP, 1% KSG-15AP, 4% KF-96A-6T, 0.15% KF-6038, 5% KF-995, 0.2% KSP-101, 46.85% water, 10% butanediol, 10% methyl methacrylate, 0.5% NaCl, 0.4% acetone, and 0.5% 1,2-hexanediol.

6. The hair loss prevention and hair strengthening agent containing recombinant type XVII collagen (Recomtein PHD) and water-soluble fullerene according to claim 1, characterized in that, The hair loss prevention and hair styling solvent containing water-soluble fullerene undergoes hydrophilic modification of the fullerene.

7. The method for preparing the anti-hair loss and hair-strengthening agent containing recombinant type XVII collagen (Recomtein PHD) and water-soluble fullerene as described in any one of claims 1 to 4, characterized in that, The freeze-dried powder and water-soluble fullerene solvent, which has the effect of preventing hair loss and strengthening hair, are immediately reconstituted before use to form an anti-hair loss essence with synergistic effect.

8. The method for preparing the anti-hair loss and hair-strengthening agent containing recombinant type XVII collagen (Recomtein PHD) and water-soluble fullerene according to claim 7, characterized in that, The preparation method of the solvent with water-soluble fullerene anti-hair loss and hair-strengthening effects includes the following steps: S1: Mix the aqueous phase components in a hot water bath, place the hot water bath in the hot water bath and heat it at 60-70℃, stirring constantly until all components are dissolved evenly; the aqueous phase components are butanediol and p-hydroxyacetophenone. S2: After cooling to room temperature, remove the other components of the aqueous phase, such as water and Starfact. ® Hydro-C60 water-soluble fullerene and 1,2-hexanediol are added to an aqueous phase pot and stirred until all ingredients are completely mixed to obtain a solvent with water-soluble fullerene anti-hair loss and hair-strengthening effects. The solvent with water-soluble fullerene anti-hair loss and hair-strengthening effects is then placed into a clean container and sealed for storage.

9. The method for preparing the anti-hair loss and hair-strengthening agent containing recombinant type XVII collagen (Recomtein PHD) and water-soluble fullerene according to claim 7, characterized in that, The method for preparing the freeze-dried powder includes the following steps: (1) Ingredients: Pre-treated Recommendin ® PHD Fuyuxin XVII Collagen Solution is sterilized by microporous membrane filtration. (2) Filling: Dispense into vials and place in a freeze dryer; (3) Pre-freezing: Turn on the freeze dryer and maintain the temperature at 4℃±1℃ for 1-4 hours; cool down to below -20℃ and maintain the freeze drying chamber temperature at -20±2℃ for 6-8 hours. (4) First sublimation: Start evacuating the vacuum. When the vacuum level reaches below 10 Pa, start heating. Adjust the temperature of the freeze-drying chamber to slowly rise to 0.0℃, with a temperature increase of no more than 1.0℃ per hour. Maintain the vacuum condition for 25-30 hours. (5) Secondary sublimation: Continue to adjust the temperature of the freeze-drying chamber, raising it to 30-35℃ at a rate of 3-10℃ / h. Keep warm for 3-5 hours. (6) Discharge: Inert gas is introduced, the freeze-drying chamber is adjusted to normal pressure, the inner plug is pressed, and the gadolinium cap is removed to obtain freeze-dried powder with the anti-hair loss and hair-strengthening effect of type XVII collagen.

10. The method for preparing the anti-hair loss and hair-strengthening agent containing recombinant type XVII collagen (Recomtein PHD) and water-soluble fullerene according to claim 9, characterized in that, The inert gas is nitrogen, helium, or argon.

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