Plant extract synergistic antibacterial disinfectant and preparation method thereof

Abstract

The invention belongs to the technical field of natural antibiosis and green disinfection, and discloses a plant extract synergistic antibacterial disinfectant and a preparation method thereof.The disinfectant takes sweet wormwood leaf extract, purslane herb extract and fructus cnidii fruit extract as main active ingredients, is rich in flavonoid, lactone and coumarin antibacterial ingredients, and has a synergistic antibacterial effect. And a multi-target synergistic antibacterial effect is realized through scientific compounding. The preparation does not contain alcohol and quaternary ammonium salt, chitosan oligosaccharide and glycyrrhizin are adopted as natural interface agents, and a choline-citric acid deep eutectic solvent and nano-micelles are introduced to synergistically construct an alcohol-free carrier system, so that the solubility and stability of active components are remarkably improved. The preparation method is simple, low in energy consumption and environmentally friendly, and the obtained product is mild in smell and free of skin irritation, has excellent broad-spectrum antibacterial performance and is suitable for surface disinfection of human skin, daily necessities, medical instruments and public environments.

Description

Technical Field

[0001] This invention belongs to the field of natural antibacterial and green disinfection technology, specifically relating to a plant extract synergistic antibacterial disinfectant and its preparation method. Background Technology

[0002] With increased public awareness of public health and personal protection, antibacterial disinfectants have been widely used in medical and health care, household cleaning, and personal care. Currently, most commercially available antibacterial disinfectants use alcohol, quaternary ammonium salts, chlorine-based disinfectants, or biguanides as their main active ingredients. While they offer some bactericidal and bacteriostatic effects in the short term, they also present several problems. For example, alcohol-based products are flammable and volatile, and long-term use can irritate the skin; quaternary ammonium salt disinfectants pose a high risk of residue and can easily lead to the development of drug-resistant strains; and chlorine-based disinfectants, due to their strong oxidizing properties, are corrosive and irritating, making them unsuitable for sensitive individuals and infants.

[0003] In recent years, plant-derived natural antibacterial components have become an important direction in the research and development of antibacterial agents due to their good biocompatibility, broad-spectrum antibacterial ability, and environmental friendliness. For example, artemisinin from Artemisia annua, flavonoids and polysaccharides from Portulaca oleracea, and coumarin compounds from Cnidium monnieri have all been proven to have good antibacterial activity. However, traditional plant-based antibacterial agents generally suffer from uneven release of active substances, poor stability, and weak synergy, which restricts their practical application in highly effective disinfection products. In addition, many existing plant-extracted antibacterial products still rely heavily on alcohol or strong surfactants as carriers, making it difficult to meet the green requirements of "alcohol-free, safe, and low-irritation." Therefore, developing a synergistic antibacterial disinfectant solution with plant extracts as the core and a green alcohol-free carrier system has become a key breakthrough direction for improving the application performance of natural antibacterial agents. Summary of the Invention

[0004] To address the shortcomings mentioned in the background art, the present invention aims to provide a plant extract synergistic antibacterial disinfectant and its preparation method. The main antibacterial components are artemisia annua leaf extract, purslane whole herb extract, and Cnidium monnieri fruit extract. These are combined with chitosan oligosaccharides, glycyrrhizin, a deep eutectic solvent, and nanomicelles to construct the system. The solution is alcohol-free, easy to prepare, and possesses the advantages of broad-spectrum antibacterial activity, safety, and mildness.

[0005] The objective of this invention can be achieved through the following technical solutions: A plant extract synergistic antibacterial disinfectant comprises the following raw materials in parts by weight: 5-20 parts Artemisia annua leaf extract, 5-15 parts Portulaca oleracea whole herb extract, 2-10 parts Cnidium monnieri fruit extract, 0.5-2 parts chitosan oligosaccharide, 0.2-1 part glycyrrhizin, 5-15 parts deep eutectic solvent, 0.5-3 parts poloxamer 188, and 0.1-2 parts triethanolamine buffer.

[0006] More preferably, the Artemisia annua leaf extract is rich in artemisinin lactones, the Portulaca oleracea whole herb extract is rich in flavonoids and polysaccharides, and the Cnidium monnieri fruit extract is rich in coumarin active ingredients. The preparation method of the plant extracts includes: pulverizing dried Artemisia annua leaves, Portulaca oleracea whole herb, and Cnidium monnieri fruit to below 80 mesh, adding them to an ethanol-water mixed solvent, extracting at 45-60°C for 1-3 hours, filtering, concentrating under reduced pressure, and freeze-drying to obtain the corresponding plant extract powder.

[0007] More preferably, the chitosan oligosaccharide is a chitosan oligomer with a molecular weight controlled in the range of 3000 to 8000 Da through acid hydrolysis.

[0008] More preferably, the eutectic solvent is obtained by mixing choline and citric acid in a molar ratio of 1:1 and stirring at 60°C for 30 to 60 minutes.

[0009] A method for preparing a plant extract-based synergistic antibacterial and disinfectant solution includes the following steps: S1. Slowly add the powders of Artemisia annua leaf extract, Portulaca oleracea extract and Cnidium monnieri extract to a three-necked flask in sequence, add a deep eutectic solvent, place the flask in a magnetic stirrer, and disperse it fully at room temperature to obtain a uniform mixture. S2. Add chitosan oligosaccharide and glycyrrhizin to the mixture in sequence, and continue to stir magnetically until the solid is completely dissolved to form a clear and transparent base solution; S3. Transfer the obtained base solution to a high-speed dispersion device, start high-speed shear homogenization for 10-20 minutes, and then continue magnetic stirring for 30 minutes after stopping to obtain a stable suspension; S4. Poloxamer 188 and triethanolamine buffer were added to the obtained suspension in sequence. After adjusting the pH, the suspension was placed in an ultrasonic treatment device and ultrasonically treated for 30 minutes to form a transparent and uniform nanomicelle dispersion system. S5. Transfer the obtained dispersion system to a clean dispensing tank, add deionized water, filter and sterilize using a 0.45 μm microporous membrane, aseptically fill and seal, and let stand for 72 hours to obtain the plant extract synergistic antibacterial disinfectant solution.

[0010] More preferably, after adding chitosan oligosaccharide and glycyrrhizin in step S2, the solution is stirred in a water bath at 40-50°C for 30-60 minutes until it becomes clear and transparent before proceeding to the next step.

[0011] More preferably, the triethanolamine buffer solution in step S4 is prepared by mixing triethanolamine and sodium citrate in deionized water at a molar ratio of 1:1, and adjusting the pH of the resulting system to 5.0-6.0.

[0012] More preferably, the nanomicelle system in step S4 is formed by the synergistic construction of poloxamer 188 and chitosan oligosaccharide under pH 5-6 conditions, and the resulting nanomicelles have a particle size distribution concentrated in the range of 80-150 nm.

[0013] More preferably, the disinfectant is suitable for disinfecting skin, clothing, medical devices or public contact surfaces, and is used to inhibit the growth of Staphylococcus aureus, Escherichia coli and Candida albicans, and the disinfectant has an inhibition rate of more than 90% against the above pathogens.

[0014] The beneficial effects of this invention are: The disinfectant prepared in this invention uses Artemisia annua leaves, Portulaca oleracea whole herb, and Cnidium monnieri fruit as raw materials. It extracts natural active ingredients rich in artemisinin lactones, flavonoids, polysaccharides, and coumarins. These three components exhibit a complementary and synergistic effect in their antibacterial mechanism, effectively disrupting bacterial cell walls and membranes, interfering with protein synthesis, and inhibiting the germination and reproduction of fungal spores, demonstrating excellent broad-spectrum antibacterial ability. Compared to traditional single extracts, this invention, through the compound combination of plants, significantly enhances the antibacterial effect without increasing the dosage, while reducing toxic side effects and the risk of irritation.

[0015] Furthermore, this invention introduces a deep eutectic solvent composed of choline and citric acid as a green solubilizing carrier, avoiding the use of irritating solvents such as alcohol, thus ensuring good skin-friendliness while maintaining solubility. Combined with natural interface modifiers such as chitosan oligosaccharides and glycyrrhizin, a stable microenvironment system is formed, further enhancing the dispersibility and bioavailability of the antibacterial components. By synergistically constructing a nanomicelle structure with poloxamer 188 and chitosan, not only is sustained-release of the active ingredient achieved, but the formulation also possesses good stability and transdermal adsorption capacity, prolonging the antibacterial action time. The preparation method is simple, energy-efficient, requires no complex reaction conditions, and is suitable for industrial production. Attached Figure Description

[0016] The invention will now be further described with reference to the accompanying drawings.

[0017] Figure 1 Bar chart comparing the inhibition rates of three pathogens against samples from Examples 1-3 and Comparative Examples 1-2; Figure 2 The bar chart shows the relative cell viability comparison after sample treatment in Examples 1-3 and Comparative Examples 1-2. Detailed Implementation

[0018] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0019] Example 1 I. Preparation of Extracts (1) Artemisia annua leaf extract: The dried Artemisia annua leaves were pulverized to pass through an 80-mesh sieve. 100g of the extract was weighed and added to a three-necked flask. 1000mL of 70% ethanol-water solution was added and the extract was magnetically stirred at 60℃ for 2 hours. After extraction, the extract was filtered while hot and the filtrate was collected. The extract was concentrated under reduced pressure using a rotary evaporator at 45℃ and then transferred to a freeze-drying bottle. After pre-freezing at -20℃, the extract was freeze-dried for 48 hours to obtain Artemisia annua leaf extract powder.

[0020] (2) Purslane whole herb extract: The whole purslane herb was pulverized to pass through a 60-mesh sieve. 100g was weighed and placed in a round-bottom flask. 1200mL of 60% ethanol-water solution was added. The mixture was pre-soaked at room temperature for 30 minutes. The mixture was then ultrasonically extracted in a 40℃ constant temperature water bath ultrasonic extractor for 90 minutes at a frequency of 40kHz and a power of 300W. After the extraction was completed, the extract was filtered, the residue was discarded, and the filtrate was concentrated under reduced pressure to about 250mL before freeze-drying to obtain the purslane extract powder.

[0021] (3) Cnidium monnieri fruit extract: Cnidium monnieri fruit was crushed to pass through a 100-mesh sieve, 100g was weighed and placed in a 2 L three-necked flask, 1000mL of 50% ethanol was added, and the mixture was heated and refluxed at 55℃ for 1.5 hours with a stirring speed of 300 rpm. After cooling, the mixture was filtered and the filtrate was collected. The filtrate was concentrated to 200mL under reduced pressure using a rotary evaporator. The concentrate was transferred to a freeze-drying bottle, pre-frozen at -20℃ overnight, and then freeze-dried for 48 hours to obtain the brownish-yellow Cnidium monnieri fruit extract powder.

[0022] II. Preparation of Plant Extract Synergistic Antibacterial and Disinfecting Solution The plant extract synergistic antibacterial disinfectant contains the following raw materials in parts by weight: 5 parts Artemisia annua leaf extract, 5 parts Portulaca oleracea whole herb extract, 2 parts Cnidium monnieri fruit extract, 0.5 parts chitosan oligosaccharide, 0.2 parts glycyrrhizin, 5 parts deep eutectic solvent, 0.5 parts poloxamer 188, and 0.1 parts triethanolamine buffer.

[0023] The preparation method is as follows: Weigh 5.00 g of Artemisia annua leaf extract, 5.00 g of Portulaca oleracea whole herb extract, and 2.00 g of Cnidium monnieri fruit extract, and add them sequentially to a 500 mL three-necked flask. Add 5.00 g of a pre-prepared deep eutectic solvent (choline and citric acid mixed in a 1:1 molar ratio, stirred in a 60 °C water bath for 30 minutes), allow to stand at room temperature for pre-dispersion for 30 minutes, and then magnetically stir for 30 minutes. Add 0.50 g of chitosan oligosaccharide and 0.20 g of glycyrrhizin, and stir in a 45 °C water bath for 40 minutes to obtain a clear basic solution. Transfer the basic solution to a high-speed shear apparatus, shear at 8000 rpm for 15 minutes, and continue magnetically stirring for 30 minutes to obtain a homogeneous suspension. Add 0.50 g of poloxamer 188 and 0.10 g of triethanolamine buffer, adjust the pH to 5.5, and sonicate at 40 kHz and 300 W for 30 minutes at room temperature to obtain a transparent nanomicelle dispersion system. The solution was transferred to a mixing tank, deionized water was added to bring the total volume to 100g, filtered through a 0.45μm microporous membrane, filled and sealed, and allowed to stand for 72 hours to obtain the plant extract synergistic antibacterial and disinfectant solution.

[0024] Example 2 The preparation methods for Artemisia annua leaf extract, Portulaca oleracea whole herb extract, and Cnidium monnieri fruit extract are the same as in Example 1.

[0025] The preparation method of the plant extract synergistic antibacterial disinfectant is as follows: The plant extract synergistic antibacterial disinfectant contains the following raw materials in parts by weight: 20 parts Artemisia annua leaf extract, 15 parts Portulaca oleracea whole herb extract, 10 parts Cnidium monnieri fruit extract, 2 parts chitosan oligosaccharide, 1 part glycyrrhizin, 15 parts deep eutectic solvent, 3 parts poloxamer 188, and 2 parts triethanolamine buffer.

[0026] The preparation steps of the plant extract synergistic antibacterial disinfectant solution are the same as those in Example 1. Example 3

[0027] The preparation methods for Artemisia annua leaf extract, Portulaca oleracea whole herb extract, and Cnidium monnieri fruit extract are the same as in Example 1.

[0028] The preparation method of the plant extract synergistic antibacterial disinfectant is as follows: The plant extract synergistic antibacterial disinfectant contains the following raw materials in parts by weight: 12.5 parts Artemisia annua leaf extract, 10 parts Portulaca oleracea whole herb extract, 6 parts Cnidium monnieri fruit extract, 1.25 parts chitosan oligosaccharide, 0.6 parts glycyrrhizin, 10 parts deep eutectic solvent, 1.75 parts poloxamer 188, and 1.05 parts triethanolamine buffer.

[0029] The preparation steps of the plant extract synergistic antibacterial disinfectant solution are the same as those in Example 1.

[0030] Comparative Example 1 (without poloxamer 188) The preparation methods for Artemisia annua leaf extract, Portulaca oleracea whole herb extract, and Cnidium monnieri fruit extract are the same as in Example 1.

[0031] The preparation method of the plant extract synergistic antibacterial disinfectant is as follows: The plant extract synergistic antibacterial disinfectant contains the following raw materials in parts by weight: 12.5 parts Artemisia annua leaf extract, 10 parts Portulaca oleracea whole herb extract, 6 parts Cnidium monnieri fruit extract, 1.25 parts chitosan oligosaccharide, 0.6 parts glycyrrhizin, 10 parts deep eutectic solvent, and 1.05 parts triethanolamine buffer.

[0032] The preparation of the plant extract synergistic antibacterial and disinfectant solution is the same as in Example 1, except that poloxamer 188 is not added when preparing the nano-micelle dispersion system.

[0033] Comparative Example 2 (without eutectic solvent) The preparation methods for Artemisia annua leaf extract, Portulaca oleracea whole herb extract, and Cnidium monnieri fruit extract are the same as in Example 1.

[0034] The preparation method of the plant extract synergistic antibacterial disinfectant is as follows: The plant extract synergistic antibacterial disinfectant contains the following raw materials in parts by weight: 12.5 parts Artemisia annua leaf extract, 10 parts Portulaca oleracea whole herb extract, 6 parts Cnidium monnieri fruit extract, 1.25 parts chitosan oligosaccharide, 0.6 parts glycyrrhizin, 1.75 parts poloxamer 188, and 1.05 parts triethanolamine buffer.

[0035] The preparation of the plant extract synergistic antibacterial disinfectant solution is the same as in Example 1, except that no deep eutectic solvent is added.

[0036] Performance testing 1. Quantitative determination of antibacterial rate The antibacterial effect of samples against common pathogenic bacteria was tested using the plate coating method. Standard strains of Staphylococcus aureus, Escherichia coli, and Candida albicans were selected and prepared at a concentration of 1×10⁻⁶. 6 CFU / mL bacterial suspension. Take 1 mL of each disinfectant sample from the examples and comparative examples, mix with the bacterial suspension at a 1:1 ratio, incubate at 37℃ for 30 min, then spread 100 μL onto nutrient agar plates and incubate for 24 h before counting colony-forming units (CFU). Calculate the inhibition rate using the colony count A in the blank control group and the colony count B in the sample group:

[0037] The results are shown in Table 1 below.

[0038]

[0039] As shown in Table 1, the disinfectants of Examples 1-3 of this invention exhibited significant antibacterial effects against Staphylococcus aureus, Escherichia coli, and Candida albicans, with average inhibition rates exceeding 90%, and the inhibition rates of Examples 2 and 3 exceeding 95%. In contrast, the inhibition rates of Comparative Examples 1 and 2 were significantly lower, indicating that poloxamer 188 and the eutectic solvent played a key synergistic role in the system. These results demonstrate a multi-target synergistic antibacterial mechanism among Artemisia annua, Portulaca oleracea, and Cnidium monnieri extracts, which can disrupt bacterial cell membrane structure and inhibit metabolic activity; the eutectic solvent improves the solubility and stability of the active ingredients, while the nanomicelle structure promotes their adsorption and penetration on the bacterial surface, thereby achieving highly efficient and long-lasting antibacterial performance.

[0040] 2. Determination of minimum inhibitory concentration The samples obtained in Examples 1-3 and Comparative Examples 1-2 were prepared into a series of concentration gradient solutions using sterile nutrient broth medium via a double dilution method. The concentration gradients were 1.00%, 0.50%, 0.25%, 0.125%, 0.0625%, 0.03125%, and 0.01563%. Each concentration solution was added to a 96-well microplate, with 100 μL of sample solution added to each well. The above-mentioned bacterial strains were inoculated onto nutrient agar slants and incubated at 37°C for 18-24 hours. Fresh colonies were picked and the culture temperature was adjusted to 1×10⁻⁶. 6 The target bacterial suspension was diluted with broth to a concentration of CFU / mL. 100 μL of the prepared target bacterial suspension was added to the corresponding sample solution in each well.

[0041] After sealing the microplate, place it in a 37°C incubator and incubate for 24 hours. Observe visually whether bacterial growth occurs. The lowest sample concentration that becomes clear and transparent without turbidity is the minimum inhibitory concentration (MIC) of the sample against the corresponding bacterial species. The results are expressed as percentage concentration (%), as shown in Table 2 below.

[0042]

[0043] As shown in Table 2, the minimum inhibitory concentrations (MICs) of Examples 1-3 of this invention are significantly lower than those of the comparative samples, indicating that under synergistic formulation conditions, the disinfectant can achieve highly efficient antibacterial activity at lower concentrations. Example 2 showed the best performance, with the MIC values ​​of all three strains not exceeding 0.5%, while the MICs of Comparative Examples 1 and 2 were generally greater than 1%, reaching as high as 2.5% against Candida albicans. This result fully demonstrates that the synergistic effect of Artemisia annua leaf, Portulaca oleracea, and Cnidium monnieri extracts has a multi-target antibacterial mechanism, achieving enhanced effects through cell membrane disruption and metabolic pathway interference. Simultaneously, the deep eutectic solvent improves the solubility and dispersibility of the plant active ingredients, and the nanomicelles formed by poloxamer 188 promote stable encapsulation and controlled-release delivery, allowing the active substances to be uniformly adsorbed and penetrated on the bacterial surface, thus enhancing antibacterial efficiency.

[0044] 3. Skin irritation test (in vitro substitution method) A reconstructed human epidermal (RHE) model was used, and the skin irritation of the disinfectant samples was assessed according to the OECD TG 439 method. 50 μL of samples from Examples 1-3 and Comparative Examples 1-2 were added to the surface of pre-cultured RHE models, with negative controls (PBS) and positive controls (5% sodium dodecyl sulfate). After 60 minutes of treatment, the samples were washed with PBS and transferred to fresh culture medium for incubation for 42 hours. After treatment, MTT dye was added and reacted for 3 hours. After dissolution, the optical density (OD) was measured using a microplate reader to calculate relative cell viability (relative to the negative control). If cell viability was ≥80%, it was considered non-irritating. The results are shown in Table 3 below.

[0045]

[0046]

[0047] As shown in Table 3, the relative cell viability of Examples 1-3 was all above 90%, significantly higher than the 80% non-irritating criterion, demonstrating good skin mildness. In contrast, the cell viability of Comparative Examples 1 and 2 was 74.3% and 62.8%, respectively, indicating varying degrees of irritation. The positive control showed only 32.1%, validating the model's effectiveness and sensitivity. These results demonstrate that this invention, by removing alcohol and traditional irritating antibacterial components, employing natural interface agents such as chitosan oligosaccharides and glycyrrhizin, and constructing a nanomicelle system synergistically formed by a deep eutectic solvent and poloxamer 188, allows plant extracts to maintain the physiological stability of the skin and mucous membranes while exerting antibacterial activity, thereby significantly reducing the risk of skin irritation.

[0048] 4. Clarity and Sedimentation Observation Test Samples from Examples 1-3 and Comparative Examples 1-2 were separately bottled in sealed transparent glass bottles and stored at room temperature (25±2℃) in the dark for 30 days. The appearance of the samples was observed every 7 days, and any phenomena such as layering, precipitation, or turbidity were recorded. On day 30, the transmittance and turbidity (expressed in NTU) were measured using a turbidimeter. Samples with a turbidity value below 5 NTU and no visible precipitate were considered clear and stable; samples with a turbidity value above 10 NTU or with obvious precipitate were considered unstable. The results are shown in Table 4 below.

[0049] Table 4 Clarity and Sedimentation Results

[0050] Table 4 shows that Examples 1-3 maintained good clarity after standing at room temperature for 30 days, with turbidity values ​​below 5 NTU and no precipitation, indicating a physically stable system. In contrast, Comparative Examples 1 and 2 showed turbidities of 8.4 and 11.7 NTU, respectively, with Comparative Example 2 exhibiting significant precipitation, demonstrating an unstable system structure. These results demonstrate that the present invention, by introducing a deep eutectic solvent and poloxamer 188 to construct a nano-micelle dispersion system, effectively improves the dispersibility and compatibility of multi-component plant extracts under alcohol-free conditions, preventing the aggregation and sedimentation of active ingredients due to poor solubility or interfacial inhomogeneity. Furthermore, the synergistic interfacial regulation effect of chitosan oligosaccharides and glycyrrhizin further enhances the micellar stability of the system, enabling the final product to maintain clarity and transparency even after long-term storage.

[0051] 5. Storage stability and antibacterial rate test The samples from Examples 1-3 and Comparative Examples 1-2 were respectively filled into sealed glass bottles and stored at a constant temperature (25±2℃) in the dark for 30 days. Every 30 days, samples were taken to determine their inhibition rates against Staphylococcus aureus, Escherichia coli, and Candida albicans. The diameter of the inhibition zone or colony forming units (CFU) was measured using the plate coating method, and the inhibition rate was calculated. The results are shown in Table 5 below.

[0052]

[0053] As shown in Table 5, Examples 1-3 maintained an inhibition rate of over 90% against Staphylococcus aureus, Escherichia coli, and Candida albicans after 30 days of storage. Example 2, in particular, showed an inhibition rate close to its initial value, demonstrating excellent long-term antibacterial performance. In contrast, Comparative Examples 1 and 2 showed a significant decrease in inhibition rate after storage, dropping to 67.2% and 58.4%, respectively, indicating that the active ingredients in their systems were easily degraded or precipitated during storage, leading to a decline in antibacterial ability. These results fully demonstrate that the present invention improves the solubility and stability of plant extracts through a deep eutectic solvent. The nanomicelle structure synergistically constructed from poloxamer 188 and chitosan oligosaccharides effectively encapsulates, sustains, and protects the active ingredients, slowing down their oxidative inactivation or degradation, thus endowing the formulation with good storage stability and sustained antibacterial effect, reflecting the advantages of a green, safe, and efficient product.

[0054] In the description of this specification, references to terms such as "an embodiment," "example," "specific example," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the invention. In this specification, illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0055] The foregoing has shown and described the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of the invention. Various changes and modifications can be made to the invention without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claimed invention.

Claims

1. A plant extract synergistic antibacterial disinfectant solution, characterized by, The plant extract synergistic antibacterial disinfectant liquid is prepared from the following raw materials by weight: Artemisia annua leaf extract 5-20 parts, Herba Portulacae extract 5-15 parts, Cnidium fruit extract 2-10 parts, chitosan oligosaccharide 0.5-2 parts, glycyrrhizin 0.2-1 part, deep eutectic solvent 5-15 parts, poloxamer 188 0.5-3 parts, and triethanolamine buffer 0.1-2 parts.

2. The plant extract synergistic antibacterial disinfectant solution according to claim 1, characterized in that, The Artemisia annua leaf extract is rich in artemisinin lactones, the Herba Portulacae extract is rich in flavonoids and polysaccharides, and the Cnidium fruit extract is rich in coumarin active ingredients.

3. The plant extract synergistic antibacterial disinfectant solution according to claim 1, characterized in that, The chitosan oligosaccharide is a chitosan oligomer with a molecular weight controlled in the range of 3000-8000 Da by acid hydrolysis.

4. The plant extract synergistic antibacterial disinfectant solution according to claim 1, characterized in that, The deep eutectic solvent is prepared by mixing choline and citric acid at a molar ratio of 1:1 and stirring at 60°C for 30-60 minutes.

5. A method for preparing a plant extract synergistic antibacterial disinfectant, wherein the plant extract synergistic antibacterial disinfectant is as described in any one of claims 1-4, characterized in that, The method comprises the following steps: S1. Artemisia annua leaf extract, Herba Portulacae extract, and Cnidium extract powder are sequentially and slowly added into a three-necked flask, deep eutectic solvent is added, and the mixture is fully dispersed in a magnetic stirring device at room temperature to obtain a uniform mixture; S2. Chitosan oligosaccharide and glycyrrhizin are sequentially added into the mixture, and magnetic stirring is continued until the solids are completely dissolved to form a clear and transparent base solution; S3. The obtained base solution is transferred into a high-speed dispersion device, high-speed shearing homogenization is started for 10-20 minutes, and then magnetic stirring is continued for 30 minutes to obtain a stable suspension; S4. Poloxamer 188 and triethanolamine buffer are sequentially added into the obtained suspension, the pH is adjusted, and the mixture is subjected to ultrasonic treatment in an ultrasonic treatment device for 30 minutes to form a transparent and uniform nanomicelle dispersion system; S5. The obtained dispersion system is transferred into a clean liquid preparation tank, deionized water is added, and filtration and sterilization treatment are performed using a 0.45 μm microporous filter, and then sterile filling and sealing are performed, and the plant extract synergistic antibacterial disinfectant liquid is obtained after standing for 72 hours.

6. The method of preparing a plant extract synergistic antibacterial disinfectant solution according to claim 5, characterized in that, In step S2, after the addition of chitosan oligosaccharide and glycyrrhizin, water bath stirring is performed at 40-50°C for 30-60 minutes, and the solution is formed into a clear and transparent state before entering the next step.

7. The method of preparing a plant extract synergistic antibacterial disinfectant solution according to claim 5, characterized in that, In step S4, the triethanolamine buffer is prepared by mixing triethanolamine and sodium citrate at a molar ratio of 1:1 in deionized water, and the pH of the obtained system is adjusted to 5.0-6.

0.

8. The method of preparing a plant extract synergistic antibacterial disinfectant solution according to claim 5, characterized in that, In step S4, the nanomicelle system is formed by the synergistic construction of poloxamer 188 and chitosan oligosaccharide at pH 5-6, and the particle size distribution of the obtained nanomicelles is concentrated in the range of 80-150 nm.

9. The plant extract synergistic antibacterial disinfectant solution according to claim 1, characterized in that, The disinfectant liquid is suitable for skin, clothing, medical devices, or public contact surface disinfection, and is used for inhibiting the growth of Staphylococcus aureus, Escherichia coli, and Candida albicans, and the antibacterial rates of the disinfectant liquid on the above pathogenic bacteria are all higher than 90%.

Images