Device for activity recovery and preservation of in-vitro tissue and organ sections

Abstract

The invention relates to the technical field of section activity recovery, in particular to a device for activity recovery and preservation of in-vitro tissue and organ sections. Comprising a base module, the base module comprises a base, and the base is provided with arrangement parts distributed in an array mode; the cover surface module comprises cover plates which are distributed in an array mode, and a pressure layer is arranged on the face, facing the placement part, of each cover plate; the smoothing piece comprises a concentration part located in the center of the cover plate, conduction parts distributed in an array mode are arranged on the outer side of the concentration part, and the conduction parts communicate with the concentration part; and the adsorption parts are distributed on the peripheral side of the placement part in an array mode, and when the cover plate extrudes the adsorption parts, gas in the adsorption parts can impact the central area of the placement part through the conduction part and the concentration part. By arranging the micro-pressure layer, after the cover plate and the base are connected, the micro-pressure layer fixes the slice, it is ensured that the slice is not curled up or damaged, and meanwhile the scene requirements of free liquid inlet and outlet and overall immersion liquid culture are met.

Description

Technical Field

[0001] This invention relates to the field of tissue section viability restoration technology, specifically to a device for viability restoration and preservation of ex vivo tissue and organ sections. Background Technology

[0002] In life sciences, medical diagnostics, and drug development, tissue sectioning is a core tool for exploring the relationship between the microstructure and function of biological tissues. The preparation of thin sections is highly susceptible to various types of damage. This damage not only disrupts the natural microstructure of the tissue but also directly interferes with the reliability and accuracy of subsequent experimental data. Thin sections can also cause shrinkage, affecting testing and becoming a key bottleneck restricting the reliability of research results. Current technologies still have many limitations in terms of damage repair and culture. As the requirements for experimental data accuracy in life science research continue to increase, developing more adaptable personalized sectioning techniques, developing efficient damage repair methods, and establishing suitable section culture systems have become core directions for overcoming this technological bottleneck.

[0003] Chinese patent application number CN202111468496.1 discloses a paraffin embedding device for oncology biological sections, relating to the field of oncology. This paraffin embedding device for oncology biological sections includes an embedding box with a heat-conducting plate fixedly embedded in its inner wall. Two symmetrically arranged fixing plates are slidably connected inside the embedding box, and the two fixing plates are fixedly connected by snap-fit ​​clips. A sleeve is slidably connected inside the embedding box, and a retaining ring is fixedly installed on the outer surface of the sleeve outside the embedding box. A sliding rod is slidably connected inside the sleeve, passing through both sides of the sleeve and extending to the outer side of the sleeve. This paraffin embedding device for oncology biological sections first compacts and shapes the biological tissue using the fixing plates, and then injects paraffin liquid from the center to both sides to form a complete paraffin block. This prevents the biological tissue from curling and deforming in the paraffin solution, solving the problem of overlapping and wrinkling of biological tissue caused by existing operating methods.

[0004] Although the relevant patented technologies can compact and shape biological tissues using fixation sheets, preventing them from curling and deforming in paraffin solutions, there are still some technical problems that need to be solved in practical applications.

[0005] Specifically, the preparation process of thin sections is highly susceptible to various types of damage. This damage not only disrupts the natural microstructure of the tissue but also directly interferes with the reliability and accuracy of subsequent experimental data. For ultrathin sections of 4-7 μm, applications primarily focus on fixation and morphological analysis, making it difficult to maintain the section's viability and physiological function. During the fixation process, ultrathin sections are prone to decreased cell activity, hindered substance exchange, and the creation of a harsh microenvironment, thus affecting experimental reliability.

[0006] Meanwhile, thinner sections (300-800 μm) are increasingly used in drug screening, organ-on-a-chip, and physiological function testing. These sections focus on fixation and viability maintenance to simulate the in vivo physiological environment. However, current technologies lack integrated devices that can accommodate both ultrathin and thick sections: thick sections are prone to functional degradation during culture due to shrinkage or uneven material exchange, while traditional methods such as paraffin embedding cannot provide dynamic culture support. Therefore, developing a device that can accommodate sections of different thicknesses while ensuring both fixation and maintenance of physiological activity has become crucial to overcoming this technological bottleneck. Summary of the Invention

[0007] The purpose of this invention is to provide a device for the activity restoration and preservation of ex vivo tissue and organ sections. Through the synergistic mechanism of micro-pressure control, gas impact and negative pressure adsorption, it can achieve comprehensive activity restoration and preservation of sections ranging from ultrathin to thick.

[0008] To achieve the above objectives, the present invention provides the following technical solution: This invention provides an apparatus for the viability restoration and preservation of ex vivo tissue and organ sections, comprising: A base module, comprising a base on which an array of mounting portions are provided; A cover module comprising an array of cover plates, wherein a pressure layer is provided on the side of the cover plates facing the mounting portion; A smoothing component includes a central portion located at the center of a cover plate, and an array of conductive portions distributed on the outer side of the central portion, the conductive portions being connected to the central portion; The adsorption element is located in an array distributed around the periphery of the placement section. When the cover plate squeezes it, the gas inside it can impact the central area of ​​the placement section through the conduction section and the concentration section.

[0009] Preferably, the adsorption element comprises: The cylindrical body is arranged in an array around the periphery of the mounting section, and its interior is provided with an axially movable part. The passage is located inside the moving part and is connected to the space below the moving part. After the cover plate is connected to the moving part, it is connected to the guide section. The elastic element has one end connected to the movable part and the other end connected to the cylinder.

[0010] Preferably, the base has a fixing member inside, which is connected to the adsorption member. The fixing member adsorbs and fixes the slice placed in the placement part by axial movement of the movable part.

[0011] Preferably, the fixing member includes an adsorption main tube disposed inside the base, the adsorption main tube being connected to the cylinder body, and a central tube and an edge tube being provided on the outer side of the adsorption main tube, the central tube corresponding to the central area of ​​the placement part, and the edge tube corresponding to the edge area of ​​the placement part.

[0012] Preferably, a microtube is provided on the outside of the adsorption main tube, which is used to restore the pressure state inside the adsorption main tube after the pressure layer has completed the fixation of the slice.

[0013] Preferably, the channel is provided with a switching element, which is used to change the adsorption direction of the adsorption element when the movable part moves axially.

[0014] Preferably, the switching element includes a wedge disposed inside the channel, and a ball is provided on the wedge; When the movable part moves axially downward, the ball releases its blockage of the wedge, and the area below the movable part is under positive pressure; when the movable part moves axially upward, the ball blocks the wedge, and the area below the movable part is under negative pressure.

[0015] Preferably, the bottom of the cylinder is provided with a secondary main pipe, one end of which is connected to the cylinder and the other end is connected to the adsorption main pipe.

[0016] Preferably, the top of the cylinder is provided with an exhaust pipe.

[0017] Preferably, the mounting portion has a connecting portion on its periphery, and the base is connected to the cover plate through the connecting portion; The cover plate and the non-corresponding area of ​​the mounting part are provided with hollowed-out parts.

[0018] The technical effects and advantages of this invention are as follows: 1. This invention, by setting a pressure layer, applies micro-pressure to the slides when the base and cover plate are combined. This pressure layer can smooth out the curled parts of the slides without causing tissue damage. The material of the pressure layer is close to the softness of human skin, ensuring that it will not cause pressure damage when in contact with the slides. At the same time, it continuously provides a stable but gentle fixing force throughout the culture process, preventing the slides from shifting or deforming in the liquid environment, thereby maintaining the integrity and activity of the slides.

[0019] 2. This invention enhances the flattening effect of slices by incorporating an adsorption component and a fixing component, utilizing the synergistic mechanism of gas impact and negative pressure adsorption. Gas is ejected vertically from the concentration section, acting directly on the slice surface and causing it to expand rapidly. Simultaneously, the negative pressure system adsorbs the lower surface of the slice through the central and edge tubes, generating a reverse pulling force. This synergistic mechanism of gas impact and negative pressure adsorption effectively alleviates the folding phenomenon in severely curled areas on the slice, laying a solid foundation for the subsequent secondary flattening operation of the pressure layer, and further improving the accuracy and efficiency of slice processing.

[0020] 3. This invention incorporates a switching mechanism that, when a slide needs to be removed, switches the lower region of the moving part to a negative pressure mode. In this mode, the secondary main tube adsorbs and fixes the slide through the adsorption main tube, effectively preventing the pulling effect of residual culture medium on the thin slide. This avoids the slide retraction or curling during transfer, ensuring the accuracy of subsequent operations and the safety of the slide. Attached Figure Description

[0021] Figure 1 This is a schematic diagram of the main structure of the present invention; Figure 2 This is a schematic diagram of the base module of the present invention; Figure 3 This is a schematic diagram of the structure of the cover module of the present invention; Figure 4 This is a schematic diagram of the structure of the adsorption element of the present invention; Figure 5 This is a schematic diagram of the first working state of the base module and the cover module of the present invention; Figure 6 This is a schematic diagram of the second working state of the base module and the cover module of the present invention.

[0022] In the picture: 1. Base module; 101. Base; 102. Mounting part; 103. Connecting part; 2. Cover module; 201. Cover plate; 202. Pressure layer; 203. Hollowed-out section; 3. Smoothing component; 301. Conducting part; 302. Concentrating part; 4. Adsorption component; 401. Cylinder body; 402. Moving part; 403. Elastic component; 404. Channel; 5. Switching component; 501. Wedge; 502. Sphere; 6. Fixing components; 601. Adsorption main tube; 602. Central tube; 603. Edge tube; 604. Microtube; 7. Exhaust pipe; 8. Deputy Supervisor; 9. Slice. Detailed Implementation

[0023] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are within the scope of protection of the present invention. Example 1

[0024] Reference Figures 1 to 3 As shown, a device for the viability restoration and preservation of ex vivo tissue and organ slices includes a base module 1 and a cover module 2, wherein the base module 1 includes a base 101 and an array of placement parts 102 are provided on the base 101.

[0025] It should be noted that the placement section 102 is a placement slot used to place the excised tissue and organ slides 9. The area of ​​the base 101 outside the placement section 102 is perforated with a grid pattern to ensure that liquid can quickly penetrate the base and contact the slides 9 without any blind spots.

[0026] The cover module 2 includes an array of cover plates 201. A pressure layer 202 is provided on the side of the cover plate 201 facing the placement part 102. The pressure layer 202 adopts a composite structure of "nylon mesh + silicone micro-bumps," where the nylon mesh is fixed to the lower surface of the cover plate 201 by ultrasonic welding to cover the entire placement part 102 area. On the side of the nylon mesh facing the slide 9, silicone micro-bumps (with a softness close to human skin) are evenly distributed. When the cover plate 201 is fastened to the base 101, the silicone bumps apply only micro-pressure to the slide 9 (precisely controlled by adjusting the bump height)—this smooths out wrinkles in the slide 9 without compressing the tissue and causing damage, making it suitable for easily deformable slides 9 such as cartilage, skin, and organ-on-a-chip.

[0027] Reference Figures 1 to 3 As shown, the mounting part 102 is provided with a connecting part 103 on its periphery, and the base 101 is connected to the cover plate 201 through the connecting part 103.

[0028] It should be noted that the connecting part 103 includes a magnetic block or a snap-fit ​​setting. When the cover plate 201 and the base 101 are fastened together, the connecting part 103 realizes the fixed connection between the cover plate 201 and the base 101. The connection between the two parts is achieved through the connecting part 103, which is the prior art and will not be described in detail here.

[0029] The non-corresponding areas of the cover plate 201 and the mounting part 102 are provided with perforated parts 203. When the cover plate 201 is fastened to the base 101, the perforated parts 203 and the perforated mesh of the base form a "cross-flow guide", ensuring that the liquid can quickly enter the hole from the top and side of the cover plate 201 without any dead corners in the culture.

[0030] In actual use, the operator first uses tweezers to precisely place the slide 9 into the internal area of ​​the placement section 102. Then, the cover plate 201 is fastened to the base 101. During this fastening process, the pressure layer 202 on the base 101 applies micro-pressure to the slide 9. Specifically, the silicone microbumps on the pressure layer 202 press down on the curled portion of the slide 9, causing it to gradually flatten. Because the silicone microbumps have a softness similar to human skin, the micro-pressure applied to the slide 9 when the cover and base are fastened together avoids compressive damage to the slide 9 tissue, thus ensuring the integrity and viability of the slide 9.

[0031] After the cover plate 201 and the base 101 are successfully fastened together, the staff puts the overall assembly formed by the base module 1 and the cover module 2 into the incubator. The culture medium in the incubator is divided into the placement part 102 through the hollow part 203 on the base 101 and the cover plate 201.

[0032] It is particularly noteworthy that both the base module 1 and the cover module 2 employ a fully perforated design. This design offers significant advantages when changing the culture medium, allowing for direct infusion from the top of the culture chamber or extraction from the bottom, without requiring the removal of the slide 9, thus effectively reducing the risk of contamination. Furthermore, if the incubator needs to be shaken, the micro-pressure layer 202 on the cover plate 201 will stabilize the slide 9, counteracting any slight displacement that may occur during shaking. This ensures that the slide 9 remains relatively stable in the simulated blood flow environment, which is beneficial for the culture and research of the slide 9.

[0033] The core objective of this embodiment is to provide a universal device suitable not only for the fixation of 4-7μm ultrathin sections (such as preventing curling in pathological diagnosis) but also for the culture of 300-800μm thick sections (such as physiological function simulation in organ-on-a-chip). Through micro-pressure control of the pressure layer 202, the device avoids damage during section fixation, while the perforated portion 203 allows for free liquid flow, ensuring that thick sections maintain oxygenation and nutrient exchange during culture, thereby preserving physiological function. This design enables the device to cover diverse applications from ultrathin to thick sections, improving experimental efficiency and data reliability. Example 2

[0034] While the pressure layer 202 in the aforementioned embodiments can smooth out the curled slice 9 to some extent, in practical applications, there are cases where the slice 9 curls excessively. When the curling degree of slice 9 exceeds a certain range, during the smoothing operation of the pressure layer 202, it not only fails to effectively smooth out the curled slice 9, but also causes a more severe "folding" phenomenon in the curled portion of slice 9 due to the concentrated pressure. This "folding" phenomenon not only fails to achieve the expected smoothing purpose, but may also cause further damage to slice 9, affecting subsequent processing and use. In view of this, a technical improvement is made based on Embodiment 1, and the improved technical solution is as follows: Reference Figures 1 to 6 As shown, an apparatus for the viability restoration and preservation of ex vivo tissue and organ slices also includes a smoothing component 3. The smoothing component 3 includes a concentration part 302 located at the center of the cover plate 201. The outer side of the concentration part 302 is provided with an array of conductive parts 301, which are connected to the concentration part 302.

[0035] The concentrator 302 is a groove structure specially provided on the cover plate 201. With the help of the guide section 301, the gas can be combined and discharged in the concentrator 302. The discharged gas is blown vertically towards the slice 9 in the placement groove, causing the slice 9, which was originally in a curled-up state, to gradually stretch and flatten.

[0036] The periphery of the placement section 102 is provided with an array of adsorption elements 4. When the cover plate 201 squeezes it, the gas inside can impact the central area of ​​the placement section 102 through the conduction section 301 and the concentration section 302.

[0037] Reference Figure 4 As shown, the adsorption component 4 includes a cylindrical body 401, which is arranged in an array around the placement part 102. The cylindrical body 401 has an axially movable part 402 inside. The movable part 402 has a channel 404 inside. One end of the channel 404 is connected to the space below the movable part 402. After the cover plate 201 is connected to the movable part 402, the other end of the channel 404 is connected to the conduction part 301.

[0038] The movable part 402 includes a piston part and a rod part, and the rod part can be connected to the guide part 301. When the movable part 402 moves downward, the gas inside the cylinder 401 enters the guide part 301 and the concentration part 302 through the channel 404.

[0039] An elastic element 403 is provided between the movable part 402 and the cylinder 401. One end of the elastic element 403 is connected to the movable part 402, and the other end of the elastic element 403 is connected to the cylinder 401.

[0040] Reference Figures 5 to 6 As shown, the base 101 is provided with a fixing member 6 inside. The fixing member 6 is connected to the adsorption member 4. The fixing member 6 adsorbs and fixes the slice 9 placed in the placement part 102 by the axial movement of the movable part 402.

[0041] Reference Figures 5 to 6 As shown, the fixing member 6 includes an adsorption main tube 601 disposed inside the base 101. The adsorption main tube 601 is connected to the cylinder 401. A central tube 602 and an edge tube 603 are provided on the outside of the adsorption main tube 601. The central tube 602 corresponds to the central area of ​​the placement part 102, and the edge tube 603 corresponds to the edge area of ​​the placement part 102.

[0042] Reference Figures 5 to 6 As shown, a microtube 604 is also provided on the outside of the adsorption main tube 601. The microtube 604 is used to restore the pressure state inside the adsorption main tube 601 after the pressure layer 202 has completed the fixation of the slice 9.

[0043] Reference Figures 5 to 6 As shown, the top of the cylinder 401 is provided with an exhaust pipe 7.

[0044] It should be noted that the adsorption main pipe 601 is equipped with a first one-way component, which only allows gas to enter the interior of the cylinder 401 through the adsorption main pipe 601; the exhaust pipe 7 is equipped with a second one-way component, which only allows gas to exit from the interior of the cylinder 401.

[0045] During the engagement of the cover plate 201 and the base 101, specific steps must be followed precisely to ensure that the slice 9 achieves the desired flattening effect. Specifically, the rod of the movable part 402 must first be precisely aligned with the guide part 301 on the cover plate 201. This alignment action aims to ensure a smooth and unobstructed communication path between the internal channel 404 and the guide part 301, creating conditions for subsequent gas flow.

[0046] After the rod and the guide section 301 are connected, downward pressure needs to be applied to the cover plate 201 to push it downward. During this process, the cover plate 201 will drive the connected movable section 402 to move downward synchronously. As the movable section 402 moves downward, the gas in the area below it will be guided to the concentration section 302 through the connected channel 404 and the guide section 301, and finally ejected from the concentration section 302, forming an impact airflow with a certain speed and pressure. This impact airflow will directly act on the slice 9 placed in the placement section 102. After contacting the surface of the slice 9, the airflow will diffuse to the peripheral area of ​​the slice 9, effectively flattening the originally curled-up parts around the slice 9.

[0047] It is worth noting that the base 101 adopts a hollow design. When the gas ejected from the concentration section 302 diffuses towards the peripheral area of ​​the slice 9, some of the gas will enter the hollow area of ​​the base 101 and gradually dissipate into the surrounding environment, avoiding the gas from having a reverse impact on the edge area of ​​the slice 9, thereby ensuring the stability and reliability of the flattening effect.

[0048] As the moving part 402 moves downward, a negative pressure environment is created in the area above the piston part. This negative pressure is transmitted to the lower surface of the slice 9 through the central tube 602 and the edge tube 603 on the adsorption main tube 601, generating an adsorption effect. Specifically, the central tube 602 is responsible for adsorbing and fixing the central area of ​​the slice 9, while the edge tube 603 is responsible for adsorbing and fixing the edge area of ​​the slice 9. Affected by the vertically ejected gas from the concentrator 302, the central tube 602 will first complete the adsorption and fixing of the central area of ​​the slice 9. As the gas diffuses on the upper surface of the slice 9, the edge tube 603 on the adsorption main tube 601 will simultaneously adsorb and fix the edge area of ​​the slice 9. The negative pressure generated by the edge tube 603 will exert a downward pulling force on the slice 9, causing the originally curled area to have a "reversal" effect, that is, the part that was originally bent inward will unfold outward, thereby facilitating further diffusion of gas on the upper surface of the slice 9. At the same time, the diffusion process of gas on the upper surface of the slice 9 will also have a pushing effect on the curled area, further assisting the adsorption main tube 601 in completing the flattening operation of the slice 9.

[0049] Under the synergistic effect of the concentration section 302 and the adsorption main tube 601, the slice 9 will achieve a pre-flattening effect and maintain its flattened state. When the pressure layer 202 on the cover plate 201 comes into contact with the slice 9, since the slice 9 is already in a relatively flat state, the squeezing effect of the pressure layer 202 on the slice 9 will minimize the degree of curling of the slice 9. Although there may still be some curled areas on the slice 9, the bending angle of these areas will be significantly reduced under the combined action of the central tube 602 and the edge tube 603. Therefore, during the subsequent squeezing process between the pressure layer 202 and the slice 9, the silicone micro-bumps on the pressure layer 202 will be able to perform a secondary smoothing operation on these areas that have not been completely flattened, ultimately making the slice 9 completely flat.

[0050] Since the adsorption main tube 601 is also equipped with a microtube 604, one end of the microtube 604 is connected to the adsorption main tube 601 and the other end is connected to the non-placement part 102 area. After the cover and the base 101 are docked, the external gas can enter the adsorption main tube 601 through the microtube 604 so that the gas pressure in the adsorption main tube 601 is restored to the initial state. That is, the central tube 602 and the edge tube 603 lose the negative pressure adsorption on the lower surface of the slice 9. The fixation effect of the slice 9 is all the responsibility of the pressure layer 202. This avoids the culture medium in the incubator being unable to contact the negative pressure adsorption area on the slice 9 after the overall assembly formed by the base module 1 and the cover module 2 is placed in the incubator, thus affecting the culture effect of the slice 9.

[0051] This example specifically addresses the curling problem of sections of varying thicknesses through a synergistic mechanism of gas impingement and negative pressure adsorption. Specifically, for 4-7 μm ultrathin sections, the vertical airflow in the concentration section 302 quickly flattens vulnerable areas, preventing damage from excessive pressure. For 300-800 μm thick sections, the negative pressure adsorption in the edge tube 603 ensures stability at the section edges, promotes uniform diffusion of the culture medium, and maintains the viability of deeper cells. This adaptive design allows the device to prioritize fixation and protection when processing ultrathin sections, and to prioritize functional preservation when processing thick sections, achieving multi-functionality. Example 3

[0052] In the aforementioned embodiment, the smoothing effect on the curled slice 9 was effectively enhanced by setting the adsorption element 4 and the fixing element 6. However, in practical applications, after the slice 9 completes the culturing step, it needs to be removed from the placement tank for subsequent analysis or experimental operations. During this process, a problem requiring special attention arises: since the slice 9 is cultured in the culture medium before removal, when the cover is opened, a certain amount of culture medium remains between the pressure layer 202 on the cover and the slice 9. Under the action of surface tension, this remaining culture medium may exert a pulling force on the thin slice 9. Although this pulling force is small, it is sufficient to cause micro-movements in the extremely thin slice 9. These micro-movements may further cause the slice 9 to curl, thus affecting the accuracy and reliability of subsequent experiments. Therefore, a technical improvement is made based on Embodiment 1, and the improved technical solution is as follows: Reference Figures 4 to 6 As shown, a switching element 5 is provided inside the channel 404. The switching element 5 is used to change the adsorption direction of the adsorption element 4 when the movable part 402 moves axially.

[0053] Reference Figures 4 to 6 As shown, the switching component 5 includes a wedge 501 disposed inside the channel 404, and a ball 502 is provided on the wedge 501.

[0054] When the movable part 402 moves axially downward, the ball 502 releases its blockage of the wedge 501, and the area below the movable part 402 is under positive pressure; when the movable part 402 moves axially upward, the ball 502 blocks the wedge 501, and the area below the movable part 402 is under negative pressure.

[0055] Reference Figures 5 to 6 As shown, a secondary main pipe 8 is provided at the bottom of the cylinder 401. One end of the secondary main pipe 8 is connected to the cylinder 401, and the other end is connected to the adsorption main pipe 601.

[0056] It should be noted that the secondary pipe 8 is equipped with a third one-way component, which only allows gas to enter the interior of the cylinder 401 through the secondary pipe 8.

[0057] In practical applications, after the docking operation between the rod and the conductive part 301 is completed, a downward force needs to be applied to the cover plate 201. Under the push of this force, the movable part 402 will move downward. During the downward movement of the movable part 402, the gas pressure in the area below the movable part 402 will change, thereby pushing the sphere 502 upward. After the sphere 502 moves upward, the original blocking state of the wedge 501 is released. At this time, the gas in the area below the movable part 402 can enter the conductive part 301 through the channel 404, and further converge to the concentration part 302, and finally be ejected at high speed from the concentration part 302. This gas ejection method can exert a force on the slice 9, thereby realizing the flattening operation of the slice 9, ensuring that the slice 9 is in a flat state, and providing a good foundation for subsequent experiments.

[0058] After the cover plate 201 and the base 101 are fully engaged, the sphere 502 will fall back to the wedge 501 position under its own gravity, thus sealing the wedge 501 again. This design effectively prevents gas leakage under unnecessary circumstances and ensures the stability and controllability of gas flow inside the device.

[0059] It should be noted that, in this embodiment, the sphere 502 can be connected to the movable part 402 via a spring or other suitable connector. This connection method effectively prevents the sphere 502 from detaching from the channel 404 due to excessive impact force when the gas exerts an impact on it, thus ensuring the normal operation and stability of the device.

[0060] When the cover is opened, the movable part 402 is subjected to the elastic restoring force of the elastic element 403 and moves towards the top of the cylinder 401. During the upward movement of the movable part 402, a positive pressure environment is formed in the area above the piston, causing the gas inside the cylinder 401 to be discharged through the exhaust pipe 7. At the same time, a negative pressure is formed in the area at the bottom of the movable part 402. Since one end of the secondary main pipe 8 is connected to the adsorption main pipe 601 and the other end is connected to the cylinder 401, and a first one-way element is provided at the connection between the adsorption main pipe 601 and the cylinder 401, when the movable part 402 moves upward, the negative pressure generated inside the cylinder 401 will act on the lower surface of the slice 9 through the secondary main pipe 8 and the adsorption main pipe 601, thereby adsorbing and fixing the slice 9. This adsorption and fixing method can effectively avoid the pulling effect of the liquid surface tension between the cover and the slice 9 on the thin slice 9 when the cover is opened, thereby preventing the slice 9 from curling up and ensuring the accuracy and reliability of subsequent experiments.

[0061] Furthermore, since the adsorption main tube 601 is also equipped with a microtube 604, one end of the microtube 604 is connected to the adsorption main tube 601, and the other end is connected to the non-placement section 102 area. When the moving section 402 returns to its initial state, external gas can enter the adsorption main tube 601 through the microtube 604. This process allows the gas pressure in the adsorption main tube 601 to return to its initial state, thereby causing the central tube 602 and the edge tube 603 to lose their negative pressure adsorption effect on the lower surface of the slice 9. At this time, the staff can easily use tweezers to remove the cultured slice 9 from the placement section 102, facilitating subsequent operations.

[0062] Finally, it should be noted that the above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

[0063] Although embodiments of the invention have been shown and described, those skilled in the art will recognize that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A device for ex vivo tissue organ section activity recovery and preservation, characterized by, The application relates to a base module, a cover module, a smoothing piece, a suction accessory and a fixing piece. The base module comprises a base provided with an array of installation parts; The cover module comprises an array of cover plates provided with a pressure layer on one side facing the installation parts; The smoothing piece comprises a central part in the center of the cover plate, and an array of through parts on the outer side of the central part; The suction accessory is arranged on the peripheral side of the array of installation parts, and when the cover plate is pressed against the suction accessory, the internal gas can impact the central area of the installation part through the through parts and the central part.

2. The apparatus for recovery and preservation of ex vivo tissue organ slices activity according to claim 1, wherein, The suction accessory comprises: A cylinder body arranged on the peripheral side of the array of installation parts, and provided with a movable part axially movable in the interior; A channel arranged in the interior of the movable part and connected with the space below the movable part, and connected with the through parts when the cover plate is connected with the movable part; An elastic member connected with one end of the movable part and the other end of the cylinder body.

3. The apparatus for recovery and preservation of ex vivo tissue organ slices activity according to claim 2, characterized in that: The interior of the base is provided with a fixing piece connected with the suction accessory, and the fixing piece can fix the section placed in the installation part through the axial movement of the movable part.

4. The apparatus for recovery and preservation of ex vivo tissue organ slices activity according to claim 3, wherein, The fixing piece comprises a suction main pipe arranged in the interior of the base and connected with the cylinder body, and the outer side of the suction main pipe is provided with a central pipe corresponding to the central area of the installation part and an edge pipe corresponding to the edge area of the installation part.

5. The apparatus for recovery and preservation of ex vivo tissue organ slices activity according to claim 4, wherein, The outer side of the suction main pipe is further provided with a micro pipe for restoring the pressure state in the suction main pipe after the pressure layer fixes the section.

6. The apparatus for recovery and preservation of ex vivo tissue organ slices activity according to claim 5, wherein, The interior of the channel is provided with a switching piece for changing the suction direction of the suction accessory when the movable part axially moves.

7. The apparatus for recovery and preservation of ex vivo tissue organ slices activity according to claim 6, wherein, The switching piece comprises a wedge part arranged in the interior of the channel and provided with a ball; When the movable part axially moves downwards, the ball releases the blockage of the wedge part, and the lower area of the movable part is in a positive pressure state; when the movable part axially moves upwards, the ball blocks the wedge part, and the lower area of the movable part is in a negative pressure state.

8. The apparatus for recovery and preservation of ex vivo tissue organ slices activity according to claim 7, wherein, The bottom of the cylinder body is provided with a secondary main pipe connected with one end of the cylinder body and the other end of the suction main pipe.

9. The apparatus for recovery and preservation of ex vivo tissue organ slices activity according to claim 8, wherein, The top of the cylinder body is provided with an exhaust pipe.

10. The apparatus for recovery and preservation of ex vivo tissue organ slices activity according to claim 9, wherein, The peripheral side of the installation part is provided with a connecting part, and the base is connected with the cover plate through the connecting part; The non-corresponding area of the cover plate and the installation part is provided with a hollow part.

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