Light source setting method of AI camera in animal experiment system

Abstract

The invention discloses a light source setting method of an AI camera in an animal experiment system, the animal experiment system comprises an experiment box body and an animal observation box arranged in the experiment box body, and the periphery of the animal observation box is of a transparent plate structure; a plane mirror is obliquely arranged around at least one side of the animal observation box, and a camera is arranged above the experiment box body corresponding to the plane mirror; the method comprises the following steps: arranging a light source above an experimental box body, wherein the light source emits vertical light to illuminate the area of an animal observation box; anti-dazzling screens are arranged at the upper ends of the transparent plate structures on the periphery of the animal observation box, and the anti-dazzling screens prevent vertical light from directly irradiating the inner and outer side surfaces of the transparent plate structures; imaging of the vertical optics in the animal observation box in the horizontal direction enters the camera from the plane mirror to be shot and collected. The relative brightness of the impurities is reduced through the anti-dazzling screen, so that the impurities are not obvious in imaging, and the accuracy of animal behavior analysis is facilitated.

Description

Technical Field

[0001] This invention relates to the field of animal experimental chamber technology, and in particular to a method for setting the light source of an AI camera in an animal experimental system. Background Technology

[0002] The open field test (OFT) is a commonly used method in animal behavior testing. For example, it can detect spontaneous activity and movements in mice after drug administration, and is a method for evaluating the behavior, motor agility, and stress levels of experimental animals under the influence of drugs. The OFT has a wide range of applications, spanning small animal behavioral research and extending to neurological and pharmacological research. Taking antipsychotic drugs as an example, OFT is generally used to detect drug efficacy and side effects. Currently, OFT is often used as a control to reflect various spontaneous behaviors in experimental animals. Because of its simple operation and abundant data, a single experiment can quantitatively evaluate an animal's spontaneous activity, behavior, and anxiety or depression, making it widely used in drug research and development.

[0003] Open-box experiments (OFT) typically use specialized animal testing chambers, such as standard mouse cages, containing one or more mice. In pharmacological studies, mice are fed or injected with appropriate drugs, and their specific behaviors and postures are observed. Statistical results are compiled over a certain period to understand the drug's effects on the animals, pharmacokinetic behavior, and side effects, providing a relatively accurate and objective assessment of drug efficacy. Systems with automated analysis capabilities are now available on the market. These systems utilize high-definition cameras to record and identify various animal behaviors in real time, generating corresponding reports, reducing manual workload, and improving the accuracy of observations.

[0004] The lighting system in an animal experiment system needs to maintain the physiological requirements of the animals and be able to simulate various experimental conditions. At the same time, it is necessary to observe the behavior of the animals from all sides during the experiment. Therefore, the experimental chamber should avoid obstructing the observation of the animals from all sides as much as possible, and the existing light source settings need to be further optimized. Summary of the Invention

[0005] The technical problem to be solved by the present invention is to provide a method for setting the light source of an AI camera in an animal experiment system, which enables the dust on the periphery of the animal observation box inside the animal experiment box to affect the camera imaging.

[0006] To solve the aforementioned technical problem, the technical solution of the present invention is as follows:

[0007] This invention provides a method for setting the light source of an AI camera in an animal experiment system. The animal experiment system includes an experimental chamber and an animal observation box disposed in the experimental chamber. The animal observation box is made of transparent sheet metal on all sides. A plane mirror is obliquely disposed around at least one side of the animal observation box, and a camera is disposed above the experimental chamber corresponding to the plane mirror.

[0008] The method includes:

[0009] A light source is installed above the experimental chamber, and the light source emits vertical light to illuminate the area of ​​the animal observation box;

[0010] A light-shielding sheet is provided at the upper end of the transparent plate structure around the animal observation box. The light-shielding sheet blocks the vertical light from directly shining on the inner and outer surfaces of the transparent plate structure.

[0011] The vertical optics, within the animal observation box, project images horizontally through the plane mirror into the camera for image capture.

[0012] Furthermore, the light source is a high-frequency flicker-free LED light, and multiple high-frequency flicker-free LED lights form a uniform vertical light beam in the animal observation box area.

[0013] Furthermore, the method also includes: adjusting the illuminance of the high-frequency flicker-free LED lamp to 10-100 lx.

[0014] Furthermore, the method also includes: setting the brightness alternation time of the straight light to 12 hours, and during each brightness alternation, the illuminance of the straight light decreases from the current illuminance to 10 lx after 30-60 minutes.

[0015] Furthermore, the method also includes setting the color temperature of the linear light rays to 2700–5000K warm white light or neutral white light.

[0016] Furthermore, the method also includes: setting a plurality of the high-frequency flicker-free LEDs above the inner wall of the experimental chamber to form a light array, and setting a softbox below the light array.

[0017] Specifically, the transparent sheet structure is a transparent glass sheet or a transparent plastic sheet.

[0018] Using the aforementioned technical solution, the light source setting method for the AI ​​camera in the animal experiment system of the present invention uses a light source to form vertical light to illuminate the animal observation box. A light-shielding plate is set on the upper part of the transparent plate structure around the animal observation box to prevent the vertical light from illuminating dust, dirt, and other impurities on the surface of the transparent plate structure, thereby preventing them from causing significant interference on the imaging path. The light-shielding plate reduces the relative brightness of the impurities, making them less noticeable in the image, which is beneficial to the accuracy of animal behavior analysis. Attached Figure Description

[0019] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0020] Figure 1 A flowchart of a method for setting the light source of an AI camera in an animal experiment system is provided for embodiments of the present invention.

[0021] Figure 2 A schematic diagram of an experimental chamber for providing a method for setting the light source of an AI camera in an animal experimental system according to an embodiment of the present invention. Detailed Implementation

[0022] The specific embodiments of the present invention will be further described below with reference to the accompanying drawings. It should be noted that these descriptions are for the purpose of aiding understanding the present invention, but do not constitute a limitation thereof. Furthermore, the technical features involved in the various embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other.

[0023] Example 1

[0024] like Figure 1 , 2 As shown, this embodiment of the invention provides a method for setting the light source of an AI camera in an animal experiment system. The animal experiment system includes an experimental box 10 and an animal observation box 20 disposed inside the experimental box 10. The animal observation box 20 has a transparent plate structure on all sides. A plane mirror 11 is obliquely disposed around at least one side of the animal observation box 20, and a camera 12 is disposed above the experimental box 10 corresponding to the plane mirror 11.

[0025] The method includes:

[0026] S101. A light source 13 is installed above the experimental chamber 10, and the light source 13 emits vertical light to illuminate the area of ​​the animal observation box 20;

[0027] S102. A light-shielding sheet 21 is provided on the upper end of the transparent plate structure around the animal observation box 20. The light-shielding sheet 21 blocks the vertical light from directly shining on the inner and outer surfaces of the transparent plate structure.

[0028] S103, The vertical optics image in the horizontal direction within the animal observation box 20 enters the camera 12 through the plane mirror 11 for image capture.

[0029] The light source 13 is a high-frequency flicker-free LED light, and multiple high-frequency flicker-free LED lights form a uniform vertical light beam in the area of ​​the animal observation box 20.

[0030] The method further includes adjusting the illuminance of the high-frequency flicker-free LED lamp to 10-100 lx.

[0031] The method further includes setting the light-dark transition time of the straight-line light beam to 12 hours, and reducing the illuminance of the straight-line light beam from the current illuminance to 10 lx after 30-60 minutes during each light-dark transition. It is equipped with a programmable timed dimming system to precisely control the light cycle and light-dark transition time, supporting personalized settings for experimental procedures.

[0032] The method further includes setting the color temperature of the linear light rays to 2700–5000K, which is either warm white light or neutral white light. Warm white light or neutral white light of 2700–5000K is preferred to simulate the natural sunlight spectrum, while high color temperature cool white light (>6000K) should be avoided to prevent interference with melatonin secretion in animals. Prolonged exposure to monochromatic light (such as pure blue light or pure red light) should be avoided, as blue light can easily damage the retina of rodents, and red light can interfere with circadian rhythm perception; the experimental light source must have continuous spectral characteristics.

[0033] The method further includes: setting multiple high-frequency flicker-free LEDs above the inner wall of the experimental chamber to form a light array, and placing a softbox below the light array. This ensures uniform illumination and no glare in the experimental area. For video tracking experiments, stable light intensity is required to avoid changes in light affecting image recognition accuracy.

[0034] Specifically, the transparent panel structure is a transparent glass panel or a transparent plastic panel. This ensures that the mouse's movements are unobstructed from all sides, allowing for easy observation of the mouse's behavior from all directions.

[0035] The light source setting method of the AI ​​camera in the animal experiment system of the present invention uses a light source to form vertical light to illuminate the animal observation box. A light shield is set on the upper part of the transparent plate structure around the animal observation box to prevent the vertical light from illuminating dust, dirt and other impurities on the surface of the transparent plate structure, thereby preventing them from causing obvious interference in the imaging path. The light shield reduces the relative brightness of the impurities, making them less noticeable in the image, which is beneficial to the accuracy of animal behavior analysis.

[0036] The embodiments of the present invention have been described in detail above with reference to the accompanying drawings, but the present invention is not limited to the described embodiments. For those skilled in the art, various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principles and spirit of the present invention, and these variations still fall within the protection scope of the present invention.

Claims

1. A method for setting the light source of an AI camera in an animal experimental system, the animal experimental system comprising an experimental chamber and an animal observation box disposed within the experimental chamber, the animal observation box having a transparent sheet metal structure on all sides; a plane mirror is obliquely disposed around at least one side of the animal observation box, and a camera is disposed above the experimental chamber corresponding to the plane mirror; characterized in that, The method includes: A light source is installed above the experimental chamber, and the light source emits vertical light to illuminate the area of ​​the animal observation box; A light-shielding sheet is provided on the upper part of the transparent plate structure around the animal observation box. The light-shielding sheet blocks the vertical light from directly shining on the inner and outer surfaces of the transparent plate structure. The vertical optics, within the animal observation box, project images horizontally through the plane mirror into the camera for image capture.

2. The light source setting method for the AI ​​camera in the animal experiment system according to claim 1, wherein the light source is a high-frequency flicker-free LED light, and a plurality of the high-frequency flicker-free LED lights form uniform vertical light in the animal observation box area.

3. The method for setting the light source of the AI ​​camera in the animal experiment system according to claim 1, the method further includes: Adjust the illuminance of the high-frequency flicker-free LED lamp to 10-100 lx.

4. The method for setting the light source of the AI ​​camera in the animal experiment system according to claim 1, the method further includes: The alternation time between light and dark of the straight light line is set to 12 hours. During each alternation, the illuminance of the straight light line decreases from the current illuminance to 10 lx after 30-60 minutes.

5. The method for setting the light source of an AI camera in an animal experiment system according to claim 1, the method further includes: The color temperature of the linear light ray is set to warm white light or neutral white light of 2700–5000K.

6. The method for setting the light source of an AI camera in an animal experiment system according to claim 1, the method further includes: Multiple high-frequency flicker-free LEDs are arranged above the inner wall of the experimental chamber to form a light array, and a softbox is placed below the light array.

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