Coffee beverage with anti-fatigue effect and preparation method thereof

Abstract

The invention discloses a coffee beverage with an anti-fatigue effect and a preparation method of the coffee beverage. The coffee beverage with the anti-fatigue effect is prepared from the following raw materials in parts by weight: 20-40 parts of fructus cannabis, 10-20 parts of coffee beans, 0.8-1.2 parts of taurine, 3-8 parts of fructus phyllanthi, 3-5 parts of licorice root fluid extract, 1-2 parts of L-cysteine and 15-25 parts of sugar. According to the coffee beverage with the anti-fatigue effect, the raw materials and the dosage are limited, so that the coffee beverage has the fragrance of the fried hemp seeds, the fragrance of the fried coffee and the natural fragrance of the fructus phyllanthi, has natural and pure sweet fragrance after being mixed, is palatable in sweet and sour, strong, mellow, bitter and astringent without outcrop, and has outstanding flavors of the hemp seeds, the coffee and the olives. Meanwhile, the wine has excellent functions and effects of resisting fatigue, resisting aging, relieving chronic pain and the like of plant raw materials in the wine.

Description

Technical Field

[0001] This invention relates to the field of beverage technology, specifically to a coffee beverage with anti-fatigue effects and its preparation method. Background Technology

[0002] Fatigue is a very common physiological phenomenon, referring to a decline in the body's ability to maintain its physiological functions at a specific level and / or maintain a predetermined intensity of exercise. Common causes of fatigue include excessive eye strain, obesity, lack of exercise, sleep problems, psychological issues, and medications. Fatigue can be divided into physiological fatigue and pathological fatigue. Physiological fatigue is generally caused by overexertion, strenuous exercise, insufficient sleep, or unscientific weight loss methods, and can be relieved with adequate rest. Pathological fatigue, however, is not easily relieved by rest. Excluding wasting diseases, it often belongs to "chronic fatigue syndrome," characterized by a prolonged, unexplained feeling of intense fatigue or physical discomfort without any organic lesions. Prolonged exposure to this type of fatigue can lead to nervous system disorders, cardiovascular and cerebrovascular problems, digestive system discomfort, endocrine disorders, and decreased immunity, resulting in insomnia, hypertension, indigestion, and other diseases. Therefore, providing appropriate intervention for individuals experiencing pathological fatigue is essential.

[0003] Caffeine is a xanthine alkaloid compound and a central nervous system stimulant. It stimulates the cerebral cortex, leading to increased alertness, relief from fatigue, and improved work efficiency, sometimes even eliminating the feeling of fatigue. It also promotes metabolism and fat burning; moderate consumption can temporarily boost energy and mental agility. In North America, 90% of adults consume caffeine daily.

[0004] In view of this, the present invention is hereby proposed. Summary of the Invention

[0005] The purpose of this invention is to provide a coffee beverage with anti-fatigue effects and its preparation method. By limiting the ingredients and their amounts, the coffee beverage prepared by this invention has excellent anti-fatigue properties and can significantly improve fatigue symptoms.

[0006] In order to achieve the above-mentioned objectives of the present invention, the following technical solution is adopted:

[0007] The first aspect of this invention provides a coffee beverage with anti-fatigue effects, said coffee beverage being prepared from raw materials comprising the following parts by weight:

[0008] 20-40 parts hemp seeds, 10-20 parts coffee beans, 0.8-1.2 parts taurine, 3-8 parts Yunnan olives, 3-5 parts licorice extract, 1-2 parts L-cysteine, and 15-25 parts sugar.

[0009] Preferably, the coffee beverage with anti-fatigue effects is made from raw materials comprising the following parts by weight:

[0010] 25-35 parts hemp seed, 12-18 parts coffee bean, 0.8-1.0 parts taurine, 4-6 parts Yunnan olive, 4-5 parts licorice fluid extract, 1.2-1.8 parts L-cysteine, and 18-22 parts sugar.

[0011] Preferably, the coffee beverage with anti-fatigue effects is made from raw materials comprising the following parts by weight:

[0012] 30 parts hemp seeds, 15 parts coffee beans, 1 part taurine, 5 parts Yunnan olives, 4 parts licorice extract, 1.5 parts L-cysteine, and 20 parts sugar.

[0013] Preferably, the sugar includes white sugar and granulated sugar.

[0014] A second aspect of the present invention provides a method for preparing a coffee beverage with anti-fatigue effects, the method comprising the following steps:

[0015] (a) The roasted hemp seeds are crushed to obtain hemp seed powder. Then, an ethanol solution is added to the hemp seed powder and ultrasonic extraction is performed. The mixture is filtered, the filtrate is collected and concentrated until no ethanol is present to obtain the first concentrate.

[0016] (b) The first concentrate is added to the mixture of polyethylene glycol and β-cyclodextrin and stirred. After filtration and concentration, the second concentrate is obtained, wherein the second concentrate has the same mass as hemp seeds.

[0017] (c) The roasted coffee beans are crushed to obtain coffee powder. Licorice extract is added to the coffee powder, followed by ethanol solution for ultrasonic extraction. Then, after standing, filtering, and concentration until no ethanol remains, a third concentrate is obtained.

[0018] (d) Place Yunnan olives in an ethanol solution for ultrasonic extraction, then soak for a period of time, filter, and concentrate until no ethanol remains to obtain the fourth concentrate;

[0019] (e) Add taurine to the mixture of polyethylene glycol and β-cyclodextrin and stir, then filter to obtain a taurine solution;

[0020] (f) Dissolve the sugar in water to obtain a water-soluble syrup;

[0021] (g) Mix the second concentrate, third concentrate, fourth concentrate, taurine solution and water-soluble syrup, add diluent, then add L-cysteine ​​and let stand, filter to obtain the coffee beverage with anti-fatigue effect.

[0022] Preferably, in step (a), the ultrasonic extraction of hemp seed powder by adding an ethanol solution includes:

[0023] According to the mass ratio of hemp seed powder to ethanol solution of 1:(5-6), add ethanol solution to hemp seed powder and sonicate, filter, and obtain filtrate and residue. Add 2-4 times the mass of hemp seed powder ethanol solution to the residue and sonicate, filter, collect the filtrate, and combine the filtrates.

[0024] The concentration of the ethanol solution is 92%–98%;

[0025] The ultrasonic extraction temperature is 45–60℃, the time is 40–80 min, the ultrasonic frequency is 40–50 kHz, and the power is 40–50 W.

[0026] The filter used is a 5μm PP cotton membrane.

[0027] Preferably, in step (b), the mass ratio of the first concentrate to the mixture of polyethylene glycol and β-cyclodextrin is 1:(30-40);

[0028] The mixture of polyethylene glycol and β-cyclodextrin has a polyethylene glycol mass concentration of 15% to 20% and a β-cyclodextrin mass concentration of 8% to 10%.

[0029] The stirring time is 1-3 hours, and the temperature is 50-60℃;

[0030] The filtration process involves sequential filtration using microporous membranes of 0.45 μm and 0.1 μm.

[0031] Preferably, in step (c), the mass ratio of coffee powder to ethanol solution is 1:(4-6);

[0032] The volume concentration of the ethanol solution is 50%–60%.

[0033] The ultrasonic extraction temperature was 50–60℃, the time was 50–70 min, the ultrasonic frequency was 50–70 kHz, and the power was 55–65 W.

[0034] The settling time is 20-28 hours.

[0035] Preferably, step (c) further includes adding 2-3 times the mass of coffee powder in an ethanol solution with a volume concentration of 30%-40% to the filtered residue, ultrasonically extracting at 45-55°C for 40-50 minutes, then letting it stand for 20-28 hours, and then filtering to obtain a filtrate; then combining the two filtrates; wherein the ultrasonic frequency is 55-75 kHz and the power is 60 W.

[0036] Preferably, in step (d), the mass ratio of Yunnan olive to ethanol solution is 1:(2-3);

[0037] The volume concentration of the ethanol solution is 70%–80%.

[0038] The ultrasonic extraction temperature was 40–50℃, the time was 50–70 min, the ultrasonic frequency was 40–50 kHz, and the power was 90–110 W.

[0039] Soaking time is 5 to 9 days.

[0040] Preferably, in step (e), the mass ratio of taurine to β-cyclodextrin is 1:(0.8-1.2);

[0041] The concentration of β-cyclodextrin in the mixture of polyethylene glycol and β-cyclodextrin is 3% to 4%, and the concentration of polyethylene glycol is 6% to 8%.

[0042] The stirring time is 1–3 hours, and the temperature is 50–60℃;

[0043] In step (f), the sugar concentration of the water-soluble syrup is 20% to 30%;

[0044] In step (g), the diluent is purified water or liquor; the amount of diluent added is 0.1 to 1 times the total mass of the second concentrate, third concentrate, fourth concentrate, taurine solution and water-soluble syrup; the standing time is 60 to 80 hours.

[0045] Compared with the prior art, the beneficial effects of the present invention include at least the following:

[0046] This invention provides a coffee beverage with anti-fatigue properties. By limiting the ingredients and their quantities, the beverage combines the aromas of roasted hemp seeds, roasted coffee, and Yunnan olives. The resulting blend possesses a natural, pure, and sweet fragrance, with a balanced sweet and sour taste, a rich and mellow flavor, and minimal bitterness. The flavors of hemp seeds, coffee, and olives are prominent. Simultaneously, it exhibits the inherent functions and effects of the plant-based ingredients in the beverage, such as anti-fatigue, anti-aging, and chronic pain relief.

[0047] The preparation method of this invention can fully extract the effective components from each raw material, retain the original functions and aroma of the plant components, and make the product palatable. Detailed Implementation

[0048] The embodiments of the technical solution of the present invention will be described in detail below with reference to the examples. The following embodiments are only used to illustrate the technical solution of the present invention more clearly, and are therefore only examples, and should not be used to limit the scope of protection of the present invention.

[0049] It should be noted that, unless otherwise stated, the technical or scientific terms used in this application should have the ordinary meaning as understood by one of ordinary skill in the art to which this invention pertains.

[0050] Example 1

[0051] This embodiment is a coffee beverage with anti-fatigue effects, which is prepared from raw materials comprising the following parts by weight:

[0052] 25 parts hemp seeds, 18 parts coffee beans, 0.8 parts taurine, 6 parts Yunnan olives, 3 parts licorice fluid extract, 1.8 parts L-cysteine, and 18 parts sugar.

[0053] The preparation method of the above-mentioned coffee beverage with anti-fatigue effect includes the following steps:

[0054] (a) The roasted hemp seeds were crushed to obtain hemp seed powder. The hemp seed powder and ethanol solution were added to the hemp seed powder at a mass ratio of 1:5. The mixture was ultrasonically treated for 80 min and filtered with 5 μm PP cotton to obtain filtrate and residue. The residue was then ultrasonically treated for 80 min with 4 times the mass of hemp seed powder and filtered with 5 μm PP cotton. The filtrate was collected and combined and concentrated until no ethanol was found to obtain the first concentrate. The ultrasonic extraction temperature was 45℃, the ultrasonic frequency was 50kHz, and the power was 40W.

[0055] (b) The first concentrate was added to the mixture of polyethylene glycol and β-cyclodextrin at a mass ratio of 1:30 and stirred at 50°C for 3 hours. The mixture was then filtered and concentrated using 0.45 μm and 0.1 μm microporous membranes to obtain the second concentrate, wherein the mass of the second concentrate was the same as that of hemp seeds; the mass concentration of polyethylene glycol in the mixture of polyethylene glycol and β-cyclodextrin was 15% and the mass concentration of β-cyclodextrin was 8%.

[0056] (c) The roasted coffee beans are crushed to obtain coffee powder. Licorice extract is added to the coffee powder. Then, according to the mass ratio of coffee powder to ethanol solution of 1:4, 60% ethanol solution is added and ultrasonically extracted at 50°C for 70 min. The ultrasonic frequency is 50 kHz and the power is 55 W. After standing for 20 h, the mixture is filtered and the filtrate and residue are collected.

[0057] Add a 40% volume concentration ethanol solution (2 times the mass of coffee powder) to the filter residue, and extract by ultrasonication at 45°C for 50 min at a frequency of 55 kHz and a power of 60 W. Then, let it stand for 20 h and filter to obtain the filtrate.

[0058] The two filtrates were combined and concentrated until no ethanol remained, to obtain a third concentrate;

[0059] (d) According to the mass ratio of Yunnan olive to ethanol solution of 1:2, Yunnan olive was placed in an ethanol solution with a volume concentration of 80% for ultrasonic extraction, then soaked for 5 days, filtered, and concentrated until no ethanol was obtained to obtain the fourth concentrate. The ultrasonic extraction temperature was 40℃, the time was 70min, the ultrasonic frequency was 40kHz, and the power was 90W.

[0060] (e) Taurine was added to a mixture of polyethylene glycol and β-cyclodextrin and stirred at 60°C for 1 h. The mixture was then filtered to obtain a taurine solution, wherein the mass ratio of taurine to β-cyclodextrin was 1:0.8; the concentration of β-cyclodextrin in the mixture of polyethylene glycol and β-cyclodextrin was 3% and the concentration of polyethylene glycol was 6%.

[0061] (f) Dissolve the sugar in water to obtain a water-soluble syrup with a mass concentration of 20%;

[0062] (g) Mix the second concentrate, third concentrate, fourth concentrate, taurine solution and water-soluble syrup to obtain a mixture, then add 0.1 times the mass of the mixture of purified water, then add L-cysteine ​​and let stand for 60 hours, filter, and you will get the coffee beverage with anti-fatigue effect.

[0063] Example 2

[0064] This embodiment is a coffee beverage with anti-fatigue effects, which is prepared from raw materials comprising the following parts by weight:

[0065] 35 parts hemp seeds, 12 parts coffee beans, 1.0 part taurine, 4 parts Yunnan olives, 5 parts licorice fluid extract, 1.2 parts L-cysteine, and 22 parts sugar.

[0066] The preparation method of the above-mentioned coffee beverage with anti-fatigue effect includes the following steps:

[0067] (a) The roasted hemp seeds were crushed to obtain hemp seed powder. The hemp seed powder and ethanol solution were added to the hemp seed powder at a mass ratio of 1:6. The mixture was ultrasonically treated for 40 min and filtered with 5 μm PP cotton to obtain filtrate and residue. The residue was then ultrasonically treated for 40 min with 2 times the mass of hemp seed powder and filtered with 5 μm PP cotton. The filtrate was collected and combined and concentrated until no ethanol was found to obtain the first concentrate. The ultrasonic extraction temperature was 60℃, the ultrasonic frequency was 40kHz, and the power was 50W.

[0068] (b) The first concentrate was added to the mixture of polyethylene glycol and β-cyclodextrin at a mass ratio of 1:40 and stirred at 60°C for 1 hour. The mixture was then filtered and concentrated using 0.45 μm and 0.1 μm microporous membranes to obtain the second concentrate, wherein the mass of the second concentrate was the same as that of hemp seeds; the mass concentration of polyethylene glycol in the mixture of polyethylene glycol and β-cyclodextrin was 20% and the mass concentration of β-cyclodextrin was 10%.

[0069] (c) The roasted coffee beans are crushed to obtain coffee powder. Licorice extract is added to the coffee powder. Then, according to the mass ratio of coffee powder to ethanol solution of 1:6, 50% ethanol solution is added and ultrasonically extracted at 60°C for 50 min. The ultrasonic frequency is 70 kHz and the power is 65 W. After standing for 28 h, the mixture is filtered and the filtrate and residue are collected.

[0070] Add a 30% volume concentration ethanol solution (3 times the mass of coffee powder) to the filter residue, and extract by ultrasonication at 55°C for 40 min at a frequency of 75 kHz and a power of 60 W. Then, let it stand for 28 h and filter to obtain the filtrate.

[0071] The two filtrates were combined and concentrated until no ethanol remained, to obtain a third concentrate;

[0072] (d) According to the mass ratio of Yunnan olive to ethanol solution of 1:3, Yunnan olive was placed in ethanol solution with a volume concentration of 70% for ultrasonic extraction, then soaked for 9 days, filtered, and concentrated until no ethanol was obtained to obtain the fourth concentrate. The ultrasonic extraction temperature was 50℃, the time was 50min, the ultrasonic frequency was 50kHz, and the power was 110W.

[0073] (e) Taurine was added to a mixture of polyethylene glycol and β-cyclodextrin and stirred at 50°C for 3 hours. The mixture was then filtered to obtain a taurine solution, wherein the mass ratio of taurine to β-cyclodextrin was 1:1.2; the concentration of β-cyclodextrin in the mixture of polyethylene glycol and β-cyclodextrin was 4%, and the concentration of polyethylene glycol was 8%.

[0074] (f) Dissolve the sugar in water to obtain a water-soluble syrup with a mass concentration of 30%;

[0075] (g) Mix the second concentrate, third concentrate, fourth concentrate, taurine solution and water-soluble syrup to obtain a mixture, then add purified water equal to the mass of the mixture, then add L-cysteine ​​and let stand for 80 hours, filter, and the coffee beverage with anti-fatigue effect is obtained.

[0076] Example 3

[0077] This embodiment is a coffee beverage with anti-fatigue effects, which is prepared from raw materials comprising the following parts by weight:

[0078] 30 parts hemp seeds, 15 parts coffee beans, 1 part taurine, 5 parts Yunnan olives, 4 parts licorice extract, 1.5 parts L-cysteine, and 20 parts sugar.

[0079] The preparation method of the above-mentioned coffee beverage with anti-fatigue effect includes the following steps:

[0080] (a) The roasted hemp seeds were crushed to obtain hemp seed powder. The hemp seed powder and ethanol solution were added to the hemp seed powder at a mass ratio of 1:5. The mixture was ultrasonically treated for 60 min and filtered with 5 μm PP cotton to obtain filtrate and residue. The residue was then ultrasonically treated for 60 min with 3 times the mass of hemp seed powder and filtered with 5 μm PP cotton. The filtrate was collected and combined and concentrated until no ethanol was found to obtain the first concentrate. The ultrasonic extraction temperature was 50℃, the ultrasonic frequency was 45kHz, and the power was 45W.

[0081] (b) The first concentrate was added to the mixture of polyethylene glycol and β-cyclodextrin at a mass ratio of 1:35 and stirred at 55°C for 2 hours. The mixture was then filtered and concentrated using 0.45 μm and 0.1 μm microporous membranes to obtain the second concentrate, wherein the mass of the second concentrate was the same as that of hemp seeds; the mass concentration of polyethylene glycol in the mixture of polyethylene glycol and β-cyclodextrin was 18% and the mass concentration of β-cyclodextrin was 9%.

[0082] (c) The roasted coffee beans are crushed to obtain coffee powder. Licorice extract is added to the coffee powder. Then, according to the mass ratio of coffee powder to ethanol solution of 1:5, 55% ethanol solution is added and ultrasonically extracted at 55°C for 60 min. The ultrasonic frequency is 60 kHz and the power is 60 W. After standing for 24 h, the mixture is filtered and the filtrate and residue are collected.

[0083] Add a 30% volume concentration ethanol solution (3 times the mass of coffee powder) to the filter residue, and extract by ultrasonication at 50°C for 45 min at a frequency of 60 kHz and a power of 60 W. Then, let it stand for 24 h and filter to obtain the filtrate.

[0084] The two filtrates were combined and concentrated until no ethanol remained, to obtain a third concentrate;

[0085] (d) According to the mass ratio of Yunnan olive to ethanol solution of 1:2, Yunnan olive was placed in 75% ethanol solution for ultrasonic extraction, then soaked for 8 days, filtered, and concentrated until no ethanol was obtained to obtain the fourth concentrate. The ultrasonic extraction temperature was 45℃, the time was 60min, the ultrasonic frequency was 45kHz, and the power was 100W.

[0086] (e) Taurine was added to a mixture of polyethylene glycol and β-cyclodextrin and stirred at 55°C for 2 hours. The mixture was then filtered to obtain a taurine solution, wherein the mass ratio of taurine to β-cyclodextrin was 1:1; the concentration of β-cyclodextrin in the mixture of polyethylene glycol and β-cyclodextrin was 3.5%, and the concentration of polyethylene glycol was 6.5%.

[0087] (f) Dissolve the sugar in water to obtain a water-soluble syrup with a mass concentration of 25%;

[0088] (g) Mix the second concentrate, third concentrate, fourth concentrate, taurine solution and water-soluble syrup to obtain a mixture, then add purified water equal to the mass of the mixture, then add L-cysteine ​​and let stand for 72 hours, filter, and you will get the coffee beverage with anti-fatigue effect.

[0089] Example 4

[0090] This embodiment is a coffee beverage with anti-fatigue effects, which is prepared from raw materials comprising the following parts by weight:

[0091] 40 parts hemp seeds, 10 parts coffee beans, 1 part taurine, 8 parts Yunnan olives, 4 parts licorice fluid extract, 2 parts L-cysteine, and 15 parts sugar.

[0092] The preparation method of the above-mentioned coffee beverage with anti-fatigue effect is the same as that in Example 3.

[0093] Experimental Example

[0094] The coffee beverages with anti-fatigue effects prepared in Examples 3-4 were used for animal anti-fatigue experiments.

[0095] Thirty Kunming mice weighing 18–22g were selected, half male and half female, and randomly divided into three groups of 10 mice each: the control group (distilled water) and the experimental group (coffee drinks from Examples 3 and 4).

[0096] All three groups were continuously administered distilled water or corresponding coffee drinks by gavage once a day at a dose of 100 ml / kg / day for 5 days. The test was conducted 1 hour after the last administration.

[0097] The mice were placed individually in a cylindrical glass tank with a height of 20cm and a diameter of 14cm. The water depth in the tank was 10cm and the water temperature was (25±2)℃. The time was recorded 6 minutes after the mice entered the water, and the cumulative immobility time during the last 4 minutes was recorded (referring to the time when the mice stopped struggling in the water or appeared to be floating, with only slight limb movements to keep their heads on the surface of the water).

[0098] The results are shown in Table 1.

[0099] Table 1

[0100] Group Stationary time (s) control group 137 Example 3 36 Example 4 92

[0101] As shown in Table 1:

[0102] The coffee beverage prepared in this application embodiment can improve the anti-fatigue effect in mice. Compared with Example 4, the coffee beverage prepared in Example 3 of this application has better anti-fatigue performance.

[0103] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features therein. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention, and they should all be covered within the scope of the claims and specification of the present invention.

Claims

1. A coffee beverage with anti-fatigue properties, characterized in that, The coffee beverage with anti-fatigue effects is made from the following ingredients in parts by weight: 20-40 parts hemp seeds, 10-20 parts coffee beans, 0.8-1.2 parts taurine, 3-8 parts Yunnan olives, 3-5 parts licorice extract, 1-2 parts L-cysteine, and 15-25 parts sugar.

2. The coffee beverage with anti-fatigue effect according to claim 1, characterized in that, The coffee beverage with anti-fatigue effects is made from the following ingredients in parts by weight: 25-35 parts hemp seed, 12-18 parts coffee bean, 0.8-1.0 parts taurine, 4-6 parts Yunnan olive, 4-5 parts licorice fluid extract, 1.2-1.8 parts L-cysteine, and 18-22 parts sugar.

3. The coffee beverage with anti-fatigue effect according to claim 1, characterized in that, The coffee beverage with anti-fatigue effects is made from the following ingredients in parts by weight: 30 parts hemp seeds, 15 parts coffee beans, 1 part taurine, 5 parts Yunnan olives, 4 parts licorice extract, 1.5 parts L-cysteine, and 20 parts sugar.

4. The coffee beverage with anti-fatigue effect according to claim 1, characterized in that, The sugars include white sugar and granulated sugar.

5. The method for preparing a coffee beverage with anti-fatigue effects as described in any one of claims 1 to 4, characterized in that, Includes the following steps: (a) The roasted hemp seeds are crushed to obtain hemp seed powder. Then, an ethanol solution is added to the hemp seed powder and ultrasonic extraction is performed. The mixture is filtered, the filtrate is collected and concentrated until no ethanol is present to obtain the first concentrate. (b) The first concentrate is added to the mixture of polyethylene glycol and β-cyclodextrin and stirred. After filtration and concentration, the second concentrate is obtained, wherein the second concentrate has the same mass as hemp seeds. (c) The roasted coffee beans are crushed to obtain coffee powder. Licorice extract is added to the coffee powder, followed by ethanol solution for ultrasonic extraction. Then, after standing, filtering, and concentration until no ethanol remains, a third concentrate is obtained. (d) Place Yunnan olives in an ethanol solution for ultrasonic extraction, then soak for a period of time, filter, and concentrate until no ethanol remains to obtain the fourth concentrate; (e) Add taurine to the mixture of polyethylene glycol and β-cyclodextrin and stir, then filter to obtain a taurine solution; (f) Dissolve the sugar in water to obtain a water-soluble syrup; (g) Mix the second concentrate, third concentrate, fourth concentrate, taurine solution and water-soluble syrup, add diluent, then add L-cysteine ​​and let stand, filter to obtain the coffee beverage with anti-fatigue effect.

6. The preparation method according to claim 5, characterized in that, In step (a), adding an ethanol solution to the hemp seed powder for ultrasonic extraction includes: According to the mass ratio of hemp seed powder to ethanol solution of 1:(5-6), add ethanol solution to hemp seed powder and sonicate, filter, and obtain filtrate and residue. Add 2-4 times the mass of hemp seed powder ethanol solution to the residue and sonicate, filter, collect the filtrate, and combine the filtrates. The concentration of the ethanol solution is 92%–98%; The ultrasonic extraction temperature is 45–60℃, the time is 40–80 min, the ultrasonic frequency is 40–50 kHz, and the power is 40–50 W. The filter used is a 5μm PP cotton membrane.

7. The preparation method according to claim 5, characterized in that, In step (b), the mass ratio of the first concentrate to the mixture of polyethylene glycol and β-cyclodextrin is 1:(30-40); The mixture of polyethylene glycol and β-cyclodextrin has a polyethylene glycol mass concentration of 15% to 20% and a β-cyclodextrin mass concentration of 8% to 10%. The stirring time is 1-3 hours, and the temperature is 50-60℃; The filtration process involves sequential filtration using microporous membranes of 0.45 μm and 0.1 μm.

8. The preparation method according to claim 5, characterized in that, In step (c), the mass ratio of coffee powder to ethanol solution is 1:(4-6); The volume concentration of the ethanol solution is 50%–60%. The ultrasonic extraction temperature was 50–60℃, the time was 50–70 min, the ultrasonic frequency was 50–70 kHz, and the power was 55–65 W. The settling time is 20-28 hours.

9. The preparation method according to claim 5, characterized in that, In step (d), the mass ratio of Yunnan olive to ethanol solution is 1:(2-3); The volume concentration of the ethanol solution is 70%–80%. The ultrasonic extraction temperature was 40–50℃, the time was 50–70 min, the ultrasonic frequency was 40–50 kHz, and the power was 90–110 W. Soaking time is 5 to 9 days.

10. As described in claim 1, characterized in that, In step (e), the mass ratio of taurine to β-cyclodextrin is 1:(0.8-1.2); The concentration of β-cyclodextrin in the mixture of polyethylene glycol and β-cyclodextrin is 3% to 4%, and the concentration of polyethylene glycol is 6% to 8%. The stirring time is 1–3 hours, and the temperature is 50–60℃; In step (f), the sugar concentration of the water-soluble syrup is 20% to 30%; In step (g), the diluent is purified water or liquor; the amount of diluent added is 0.1 to 1 times the total mass of the second concentrate, third concentrate, fourth concentrate, taurine solution and water-soluble syrup; the standing time is 60 to 80 hours.