Active peptide composition with weight-reducing and fat-reducing effects

Abstract

The invention belongs to the technical field of food and medicine, and particularly discloses an active peptide composition with weight-reducing and fat-reducing effects, which is prepared from the following components in parts by weight: 25 to 38 parts of composite active peptide, 14 to 28 parts of nano-selenium, 5 to 10 parts of modified curcumin and 6 to 13 parts of auxiliary synergist, the preparation method of the modified curcumin comprises the following steps: preparing a NaCas stock solution and a curcumin ethanol solution, putting the NaCas stock solution into a glass bottle, dropwise adding the curcumin ethanol solution through a magnetic stirrer at the room temperature of 20 DEG C at the stirring speed of 1000r / min, continuously stirring for 10 minutes, centrifuging the mixture at the temperature of 4 DEG C to remove precipitate so as to obtain supernate, and drying the supernate, thereby obtaining the modified curcumin. And concentrating, freezing and drying the supernate to obtain the modified curcumin. The active peptide composition with the weight-reducing and fat-reducing effects is free of side effects, safe in components and good in effect, and the problems that existing synthetic chemical weight-reducing drugs are poor in effect and large in side effects are solved.

Description

Technical Field

[0001] This invention relates to the field of food and pharmaceutical technology, and in particular to an active peptide composition with weight loss and fat reduction effects. Background Technology

[0002] Overweight and obesity are mainly characterized by excessive accumulation of fat tissue in the body. There are many causes of overweight and obesity, including genetic and environmental factors, changes in metabolism and endocrine function, unhealthy lifestyle and dietary habits, and psychological factors. Currently, besides controlling diet and increasing exercise, there is a lack of clinically effective and minimally invasive weight loss methods for overweight and obesity.

[0003] On the other hand, while there are many types of chemical weight-loss drugs currently in use, most of them employ neurohormonal substances that control appetite as key components to suppress calorie intake. Although these substances, when combined with other weight-loss medications or liposuction, can quickly help people lose weight and become obese or overweight, these compounds have effects on similar receptors in the complex central nervous system, inevitably causing corresponding side effects. Furthermore, existing synthetic chemical weight-loss drugs are not very effective, and to improve their efficacy, long-term or excessive use is required. Long-term or excessive use can easily lead to addiction in some individuals with specific physical conditions, and may even cause adverse consequences such as anorexia.

[0004] In response to the current limitations of synthetic chemical weight-loss drugs, there is an urgent need to develop a weight-loss combination product with fewer side effects, healthier ingredients, and better results to meet market demand. Summary of the Invention

[0005] The purpose of this invention is to provide an active peptide composition with weight loss and fat reduction effects, which has no side effects, safe ingredients, and good results, thus solving the problems of poor efficacy and large side effects of existing synthetic chemical weight loss drugs.

[0006] To achieve the above objectives, the present invention provides an active peptide composition with weight loss and fat reduction effects, comprising the following components in parts by weight: 25-38 parts of complex active peptide, 14-28 parts of nano selenium, 5-10 parts of modified curcumin, and 6-13 parts of auxiliary synergist. The preparation method of modified curcumin is as follows: To prepare the NaCas stock solution, curcumin was added to anhydrous ethanol, stirred thoroughly, and centrifuged at 4°C to remove the precipitate. The obtained curcumin ethanol solution was stored at 4°C in the dark for later use. The NaCas stock solution was placed in a stoppered glass bottle, and at room temperature of 20°C, the curcumin ethanol solution was added dropwise using a magnetic stirrer at a stirring speed of 1000 r / min, and stirring was continued for 10 min. The mixture was centrifuged at 4°C to remove the precipitate and obtain the supernatant. The supernatant was then concentrated and freeze-dried to obtain modified curcumin.

[0007] Preferably, the components include the following parts by weight: 30 parts of compound active peptide, 18 parts of nano selenium, 8 parts of modified curcumin, and 10 parts of auxiliary synergist.

[0008] Preferably, the compound active peptides include soybean peptides, albumin peptides, ginseng peptides, collagen peptides, and sea cucumber peptides, wherein the molecular weights of soybean peptides, albumin peptides, ginseng peptides, collagen peptides, and sea cucumber peptides are all less than 1500 Da.

[0009] Preferably, the mass ratio of soybean peptide, albumin peptide, ginseng peptide, collagen peptide, and sea cucumber peptide is 4:3:2:2:3.

[0010] Preferably, the auxiliary synergists include rice peptide and sea buckthorn peptide, with a mass ratio of rice peptide to sea buckthorn peptide of 2:3, and the molecular weight of rice peptide is less than 500 Da.

[0011] Preferably, the concentration of the NaCas stock solution is 5 mg / mL.

[0012] Preferably, the concentration of the curcumin ethanol solution is 10 mg / mL.

[0013] Preferably, 10 mL of NaCas stock solution is placed in a stoppered glass bottle, and 0.5 mL of curcumin ethanol solution is added dropwise at room temperature (20°C).

[0014] Preferably, the centrifugation conditions are 8000 r / min and centrifugation for 8 min.

[0015] The advantages and beneficial effects of using the above-mentioned active peptide composition with weight loss and fat reduction effects in this invention are as follows: 1. This invention enhances fat reduction through the synergistic effect of five peptides in a complex of active peptides, utilizing a multi-target synergistic mechanism. Soybean peptides, by binding to bile acids to form insoluble complexes, promote cholesterol metabolism and reduce serum total cholesterol and low-density lipoprotein levels. Albumin peptides regulate the balance of fat, sugar, and protein metabolism, increasing the basal metabolic rate. Ginseng peptides promote insulin secretion, stabilize blood sugar levels, and accelerate subcutaneous fat breakdown. Collagen peptides maintain a low insulin concentration, promote the continuous breakdown and metabolism of fatty acids, increase the basal metabolic rate, and indirectly reduce body fat percentage. Sea cucumber peptides inhibit fatty acid synthase, blocking the formation of new fat. The combined action of soybean peptides and albumin peptides enhances the β-oxidation efficiency of adipocyte mitochondria. The combined use of ginseng peptides and collagen peptides reduces postprandial blood glucose fluctuations and increases the fat synthesis inhibition rate.

[0016] 2. Curcumin can help lower blood sugar and blood lipids, and improve insulin resistance. By modifying curcumin, its solubility and absorption rate can be improved. It can significantly reduce the size of lipid droplets in adipocytes, promote lipolysis and inhibit fat production. It can also regulate mitochondrial function by activating specific receptor pathways, and promote adipocyte browning and thermogenesis.

[0017] 3. This invention, by adding nano-selenium, lowers blood triglyceride and low-density lipoprotein levels, reduces fat accumulation, enhances spleen function, improves fat transport capacity, promotes fat breakdown rather than simply inhibiting absorption, increases small intestinal heat and digestive capacity, accelerates fat breakdown, regulates intestinal flora balance, and reduces lipid absorption. The bioavailability of nano-selenium is more than 5 times that of traditional selenium, as its nanoscale size and selenium ring / chain structure make it more easily absorbed by the intestines. The duration of action is prolonged through passive diffusion and sustained-release effects.

[0018] 4. Rice peptides can regulate gastrointestinal hormone secretion, slow gastric emptying and reduce appetite, activate lipases to accelerate the breakdown of triglycerides into free fatty acids, increase the activity of brown adipose cells, enhance basal metabolic rate, promote fat oxidation for energy, improve insulin sensitivity, reduce the expression of lipases, and inhibit new fat formation. Sea buckthorn peptides improve gastrointestinal motility, aid digestion and absorption, accelerate metabolic processes, promote absorption, and enhance immunity.

[0019] The technical solution of the present invention will be further described in detail below through embodiments. Detailed Implementation

[0020] The technical solution of the present invention will be further described below through embodiments.

[0021] Unless otherwise defined, the technical or scientific terms used in this invention shall have the ordinary meaning as understood by one of ordinary skill in the art to which this invention pertains.

[0022] Unless otherwise defined, all reagents, equipment and other materials used in this invention are commercially available.

[0023] Example 1 An active peptide composition with weight loss and fat reduction effects comprises the following components in parts by weight: 30 parts of compound active peptides, 18 parts of nano-selenium, 8 parts of modified curcumin, and 10 parts of adjuvant synergists. The compound active peptides include soybean peptides, albumin peptides, ginseng peptides, collagen peptides, and sea cucumber peptides, all with a molecular weight less than 1500 Da. The mass ratio of soybean peptides, albumin peptides, ginseng peptides, collagen peptides, and sea cucumber peptides is 4:3:2:2:3. The adjuvant synergists include rice peptides and sea buckthorn peptides, with a mass ratio of rice peptides to sea buckthorn peptides of 2:3, and the molecular weight of the rice peptides is less than 500 Da.

[0024] The preparation method of modified curcumin is as follows: Prepare a 5 mg / mL NaCas stock solution using deionized water and incubate at 4°C for 4 hours. Add 0.4 g curcumin to 40 mL of anhydrous ethanol, stir thoroughly, and centrifuge at 4°C (8000 rpm, 8 min) to remove the precipitate. Store the resulting curcumin ethanol solution (10 mg / mL) at 4°C protected from light for later use. Place 10 mL of the NaCas stock solution in a 15 mL stoppered glass bottle. At room temperature (20°C), add 0.5 mL of the curcumin ethanol solution dropwise using a magnetic stirrer at 1000 rpm for 10 min. Then, centrifuge the mixture at 4°C (8000 rpm, 8 min) to remove the precipitate and obtain the supernatant. Concentrate the supernatant and freeze-dry it to obtain modified curcumin.

[0025] Curcumin can help lower blood sugar and blood lipids, and improve insulin resistance. By modifying curcumin, its solubility and absorption rate can be improved. It can significantly reduce the size of lipid droplets in adipocytes, promote lipolysis and inhibit fat production. It can also regulate mitochondrial function by activating specific receptor pathways, and promote adipocyte browning and thermogenesis.

[0026] Example 2 An active peptide composition with weight loss and fat reduction effects comprises the following components in parts by weight: 25 parts of compound active peptides, 22 parts of nano-selenium, 5 parts of modified curcumin, and 8 parts of adjuvant synergists. The compound active peptides include soybean peptides, albumin peptides, ginseng peptides, collagen peptides, and sea cucumber peptides, all with a molecular weight less than 1500 Da. The mass ratio of soybean peptides, albumin peptides, ginseng peptides, collagen peptides, and sea cucumber peptides is 4:3:2:2:3. The adjuvant synergists include rice peptides and sea buckthorn peptides, with a mass ratio of rice peptides to sea buckthorn peptides of 2:3, and the molecular weight of the rice peptides is less than 500 Da.

[0027] The preparation method of modified curcumin is as follows: Prepare a 5 mg / mL NaCas stock solution using deionized water and incubate at 4°C for 4 hours. Add 0.4 g curcumin to 40 mL of anhydrous ethanol, stir thoroughly, and centrifuge at 4°C (8000 rpm, 8 min) to remove the precipitate. Store the resulting curcumin ethanol solution (10 mg / mL) at 4°C protected from light for later use. Place 10 mL of the NaCas stock solution in a 15 mL stoppered glass bottle. At room temperature (20°C), add 0.5 mL of the curcumin ethanol solution dropwise using a magnetic stirrer at 1000 rpm for 10 min. Then, centrifuge the mixture at 4°C (8000 rpm, 8 min) to remove the precipitate and obtain the supernatant. Concentrate the supernatant and freeze-dry it to obtain modified curcumin.

[0028] Example 3 An active peptide composition with weight loss and fat reduction effects comprises the following components in parts by weight: 38 parts of compound active peptides, 28 parts of nano-selenium, 9 parts of modified curcumin, and 13 parts of auxiliary synergists. The compound active peptides include soybean peptides, albumin peptides, ginseng peptides, collagen peptides, and sea cucumber peptides, all with a molecular weight less than 1500 Da. The mass ratio of soybean peptides, albumin peptides, ginseng peptides, collagen peptides, and sea cucumber peptides is 4:3:2:2:3. The auxiliary synergists include rice peptides and sea buckthorn peptides, with a mass ratio of rice peptides to sea buckthorn peptides of 2:3, and the molecular weight of the rice peptides is less than 500 Da.

[0029] The preparation method of modified curcumin is as follows: Prepare a 5 mg / mL NaCas stock solution using deionized water and incubate at 4°C for 4 hours. Add 0.4 g curcumin to 40 mL of anhydrous ethanol, stir thoroughly, and centrifuge at 4°C (8000 rpm, 8 min) to remove the precipitate. Store the resulting curcumin ethanol solution (10 mg / mL) at 4°C protected from light for later use. Place 10 mL of the NaCas stock solution in a 15 mL stoppered glass bottle. At room temperature (20°C), add 0.5 mL of the curcumin ethanol solution dropwise using a magnetic stirrer at 1000 rpm for 10 min. Then, centrifuge the mixture at 4°C (8000 rpm, 8 min) to remove the precipitate and obtain the supernatant. Concentrate the supernatant and freeze-dry it to obtain modified curcumin.

[0030] Comparative Example 1 Unlike Example 1, the compound active peptide does not include soybean peptide and albumin peptide, but the rest are the same.

[0031] Comparative Example 2 Unlike Example 1, the compound active peptide does not include ginseng peptide and collagen peptide, but the rest are the same.

[0032] Comparative Example 3 Unlike Example 1, the compound active peptide does not include sea cucumber peptide, but the rest is the same.

[0033] Comparative Example 4 The difference from Example 1 is the addition of common curcumin; all other aspects are the same.

[0034] Comparative Example 5 The difference from Example 1 is the addition of common organic selenium; all other aspects are the same.

[0035] Comparative Example 6 The difference from Example 1 is that no auxiliary synergist is added, but the rest is the same.

[0036] Verification Example Functional studies of the active peptide compositions of Examples 1-3 and Comparative Examples 1-6.

[0037] An obese mouse model was established by feeding SPF-grade male C57 mice with a high-fat diet for 10 weeks. The obese mice were randomly divided into 10 groups: a model control group and 9 treatment groups. The treatment groups contained the active peptide compositions corresponding to Examples 1-3 and Comparative Examples 1-6, with 12 mice in each group. The treatment groups were administered 0.5 g / kg of the corresponding product daily by gavage, while the model control group received an equal volume of distilled water. Mice continued to have free access to the high-fat diet during the treatment period. From the start of treatment, the mice's weight and food intake were recorded daily. At week 6, the mice were anesthetized, and abdominal fat was weighed. The average weight at different time points was calculated, and the statistical weight gain rate and food intake inhibition rate were recorded at the end of the experiment. The test results are shown in Tables 1 and 2.

[0038] Table 1 Test Results

[0039] Table 1 shows that the weight gain rate of Examples 1-3 was significantly inhibited compared with the model control group. Compared with Example 1, Comparative Examples 1-3 did not simultaneously add the five active peptides (soybean peptide, albumin peptide, ginseng peptide, collagen peptide, and sea cucumber peptide), resulting in a significantly weaker inhibition of weight gain. However, there was no significant change in the weight gain rate among Comparative Examples 1-3, indicating that the five active peptides had a good synergistic effect. Comparative Example 4 added ordinary curcumin. The weight gain rate of Comparative Example 4 was higher than that of Examples 1-3, indicating that ordinary curcumin was less effective than modified curcumin. Ordinary curcumin has poor water solubility and is not easily absorbed and utilized. Modification of curcumin improved its solubility and facilitated its absorption and utilization.

[0040] Table 2 Test Results

[0041] As shown in Table 2, Comparative Example 6 did not contain the auxiliary synergists rice peptide and sea buckthorn peptide compared to Example 1. Rice peptide can delay gastric emptying and reduce appetite by regulating the secretion of gastrointestinal hormones. Comparative Example 6 had the lowest food intake inhibition rate.

[0042] After the experimental period ended, the mice were fasted for more than 12 hours the day before sacrifice, with no restriction on water intake during the fasting period. The mice were anesthetized, and blood was collected by enucleation. Centrifuge tubes containing the mouse blood were placed in a centrifuge and centrifuged at 3600 rpm for 18 minutes at 4°C. The supernatant was then collected to test the levels of triglycerides (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL) in the mouse serum. C) High-density lipoprotein cholesterol (HDL) C) Leptin (LEP) levels, the results are shown in Table 3. TG, TC, LDL C, HDL C. Both LEP reagent kits are commercially available based on existing technology.

[0043] Table 3 Test Results

[0044] Table 3 shows that Comparative Example 1 did not contain added soy peptides or albumin peptides. Soy peptides promote cholesterol metabolism and reduce serum total cholesterol and low-density lipoprotein levels by binding to bile acids to form insoluble complexes. Albumin peptides regulate the balance of fat, sugar, and protein metabolism and increase basal metabolic rate. Comparative Example 1 had higher levels of triglycerides (TG), total cholesterol (TC), and low-density lipoprotein cholesterol. Comparative Example 6, which did not contain added rice peptides, had a higher triglyceride content than Comparative Example 5. Comparative Example 5 used ordinary organic selenium, which has a poorer absorption effect than nano-selenium and a poorer effect on the decomposition of triglycerides and low-density lipoprotein cholesterol.

[0045] Therefore, this invention employs the aforementioned active peptide composition with weight loss and fat reduction effects. Through the synergistic effect of the five peptides in the composite active peptide, the fat reduction effect is enhanced through a multi-target synergistic mechanism. By modifying curcumin, its solubility and absorption rate are improved. The addition of nano-selenium reduces the levels of triglycerides and low-density lipoprotein in the blood, reduces fat accumulation, and enhances spleen function. Rice peptides can regulate the secretion of gastrointestinal hormones, delay gastric emptying and reduce appetite, activate fat-decomposing enzymes, accelerate the decomposition of triglycerides into free fatty acids, increase the activity of brown adipose cells, and improve the basal metabolic rate.

[0046] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and not to limit them. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can still be made to the technical solutions of the present invention, and these modifications or equivalent substitutions cannot cause the modified technical solutions to deviate from the spirit and scope of the technical solutions of the present invention.

Claims

1. An active peptide composition with weight loss and fat reduction effects, characterized in that, It includes the following components in parts by weight: 25-38 parts of compound active peptide, 14-28 parts of nano selenium, 5-10 parts of modified curcumin, and 6-13 parts of auxiliary synergist; The preparation method of modified curcumin is as follows: To prepare the NaCas stock solution, curcumin was added to anhydrous ethanol, stirred thoroughly, and centrifuged at 4°C to remove the precipitate. The obtained curcumin ethanol solution was stored at 4°C in the dark for later use. The NaCas stock solution was placed in a stoppered glass bottle, and at room temperature of 20°C, the curcumin ethanol solution was added dropwise using a magnetic stirrer at a stirring speed of 1000 r / min, and stirring was continued for 10 min. The mixture was centrifuged at 4°C to remove the precipitate and obtain the supernatant. The supernatant was then concentrated and freeze-dried to obtain modified curcumin.

2. The active peptide composition with weight loss and fat reduction effects according to claim 1, characterized in that: The product comprises the following components in parts by weight: 30 parts of compound active peptide, 18 parts of nano selenium, 8 parts of modified curcumin, and 10 parts of auxiliary synergist.

3. The active peptide composition with weight loss and fat reduction effects according to claim 1, characterized in that: The complex active peptides include soybean peptides, albumin peptides, ginseng peptides, collagen peptides, and sea cucumber peptides. The molecular weights of soybean peptides, albumin peptides, ginseng peptides, collagen peptides, and sea cucumber peptides are all less than 1500 Da.

4. The active peptide composition with weight loss and fat reduction effects according to claim 3, characterized in that: The mass ratio of soybean peptides, albumin peptides, ginseng peptides, collagen peptides, and sea cucumber peptides is 4:3:2:2:

3.

5. The active peptide composition with weight loss and fat reduction effects according to claim 1, characterized in that: The adjuvants include rice peptide and sea buckthorn peptide, with a mass ratio of rice peptide to sea buckthorn peptide of 2:3, and the molecular weight of rice peptide is less than 500 Da.

6. The active peptide composition with weight loss and fat reduction effects according to claim 1, characterized in that: The NaCas stock solution concentration is 5 mg / mL.

7. The active peptide composition with weight loss and fat reduction effects according to claim 1, characterized in that: The concentration of curcumin ethanol solution is 10 mg / mL.

8. The active peptide composition with weight loss and fat reduction effects according to claim 1, characterized in that: Take 10 mL of NaCas stock solution and place it in a stoppered glass bottle. At room temperature of 20°C, add 0.5 mL of curcumin ethanol solution dropwise.

9. The active peptide composition with weight loss and fat reduction effects according to claim 1, characterized in that: The centrifugation conditions were 8000 r / min for 8 min.

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