Potato seed dressing agent with disease-resistant function

Abstract

The invention relates to the technical field of pesticide preparations, and particularly discloses a potato seed dressing agent with a disease-resistant function, which is prepared from the following raw materials in parts by weight: 8 to 12 parts of N-methoxy-N-o-tolyl-3-(furyl) propanamide, 5 to 8 parts of eugenol polyoxyethylene ether derivatives, 3 to 5 parts of salicylic acid glucoside, 6 to 10 parts of cationized modified chitosan and 2 to 4 parts of EDTA (Ethylene Diamine Tetraacetic Acid) chelated zinc. The preparation method comprises the following steps: treating raw materials; capsule preparation; mixing and granulating; and drying and screening. N-methoxy-N-tolyl-3-(furyl) propanamide is adopted to directly act on a key metabolic link of pathogenic bacteria, a plant-derived derivative plays a broad-spectrum antibacterial role, an inductive component activates a disease-resistant mechanism of potatoes, and meanwhile, cationized modified chitosan and other auxiliary components cooperate with each other, so that slow release of active components is realized, and the disease resistance of the potatoes is improved. The pathogenic bacteria are difficult to generate drug resistance, and the prevention and control effect is long-term and stable.

Description

Technical Field

[0001] This application relates to the field of pesticide formulation technology, and more specifically, to a potato seed dressing agent with disease resistance function. Background Technology

[0002] Potatoes are a widely cultivated and important food and economic crop globally. During cultivation, they are susceptible to various seed-borne and soil-borne diseases, such as late blight, black scurf, bacterial wilt, and scab. These diseases can infect plants from the seed germination stage, leading to reduced germination rates, weakened seedling growth, and in severe cases, significant yield reduction or even crop failure. Seed treatment, as a key preliminary technology for potato disease control, forms a protective system on the seed surface, directly inhibiting or killing pathogens attached to the seed surface and shallow soil layers, while providing initial protection for seed germination and seedling growth.

[0003] Currently, most existing potato disease-resistant seed treatment agents use traditional fungicides such as metalaxyl, fludioxonil, and thiamethoxam. These agents focus on directly destroying the cell membrane structure of pathogens, inhibiting enzyme system activity, or blocking metabolic processes to achieve a fungicidal effect. However, these chemical seed treatment agents rely on a single fungicidal mechanism and lack synergistic design between components. Long-term use can easily lead to the development of drug resistance in pathogens, causing the control effect of the agents to decline year by year. Summary of the Invention

[0004] To address the problem that long-term use of existing potato disease-resistant seed dressing agents can easily lead to pathogen resistance, resulting in a gradual decline in the effectiveness of the agents, this application provides a potato seed dressing agent with disease-resistant functions.

[0005] This application provides a potato seed treatment agent with disease resistance function, which adopts the following technical solution: Firstly, this application provides a potato seed dressing agent with disease resistance function, using the following technical solution: A potato seed dressing agent with disease resistance function comprises the following raw materials in parts by weight: 8-12 parts of N-methoxy-N-o-tolyl-3-(furanyl)propionamide, 5-8 parts of eugenol polyoxyethylene ether derivative, 3-5 parts of salicylic acid glycoside, 6-10 parts of cationic modified chitosan, 2-4 parts of EDTA chelated zinc, 3-6 parts of polyethylene glycol-400, 0.5-1.0 parts of tert-butylhydroquinone, and 54-72.5 parts of anhydrous sodium sulfate.

[0006] By adopting the above technical solution, a multi-component core system is constructed using N-methoxy-N-o-tolyl-3-(furanyl)propamide, eugenol polyoxyethylene ether derivatives, and salicylic acid glycosides. N-methoxy-N-o-tolyl-3-(furanyl)propamide directly acts on the key metabolic links of pathogens, plant-derived derivatives exert broad-spectrum antibacterial effects, and inducing components activate the potato's own disease resistance mechanism. At the same time, cationic modified chitosan and other auxiliary components work synergistically to improve the adhesion stability of each active ingredient on the seed surface and achieve slow release of active ingredients, maintaining a long-term effective concentration. The components work together to form a three-dimensional protective system. Therefore, the pathogens are unlikely to develop resistance and the control effect is stable over a long period of time. This solves the problem that existing potato disease-resistant seed dressing agents are prone to developing resistance in pathogens with long-term use, resulting in a gradual decline in the control effect of the agents.

[0007] Preferably, the cationic modified chitosan has a degree of substitution of 0.8-1.2 and a molecular weight of 50,000-80,000 Da, and the preparation method of the eugenol polyoxyethylene ether derivative includes the following steps: Eugenol and ethylene oxide are reacted at 120-140℃ and 0.3-0.5MPa pressure for 4-6 hours under the action of an alkaline catalyst to obtain the reactant. The molar ratio of eugenol to ethylene oxide is 1:10-15, and the amount of alkaline catalyst is 0.5-1.0% of the mass of eugenol. The reactants were purified by vacuum distillation to obtain eugenol polyoxyethylene ether derivatives with a degree of polymerization of 10-15.

[0008] By adopting the above technical solution, the cationic modified chitosan with a specific degree of substitution and molecular weight forms a synergistic effect with the eugenol polyoxyethylene ether derivative prepared by the process. This not only ensures the structural stability and antibacterial activity of the plant-derived derivative, but also improves its compatibility with other components. At the same time, the cationic properties of the modified chitosan enhance its binding force with the seed surface. The combination of the two enables the active ingredients to form a uniform protective layer on the seed surface, further optimizing the broad-spectrum antibacterial effect and adhesion durability.

[0009] Preferably, the preparation method of the N-methoxy-N-o-tolyl-3-(furanyl)propionamide includes the following steps: 3-(furanyl)propionic acid and o-toluidine were reacted at 25-35°C for 8-10 h under the catalysis of dicyclohexylcarbodiimide to obtain the catalytic reactant. The molar ratio of 3-(furanyl)propionic acid to o-toluidine was 1:1.1-1.3. A methylating agent was added to the catalytic reactants, and the reaction was continued at 40-50℃ for 6-8 hours. After recrystallization and purification, N-methoxy-N-o-tolyl-3-(furanyl)propionamide was obtained. The amount of the methylating agent added was 1.2-1.5 times the molar amount of o-toluidine.

[0010] By adopting the above technical solution and controlling the preparation process of raw material ratio, reaction temperature and time, the high purity and stable activity of N-methoxy-N-o-tolyl-3-(furanyl)propionamide as a novel amide fungicide are ensured. It can effectively act on the key metabolic links of pathogens, while reducing the potential impact on seed germination. When combined with plant-derived derivatives and inducing components, it can further broaden the disease resistance spectrum and improve the stability of the control effect.

[0011] Secondly, this application provides a method for preparing a potato seed dressing agent with disease resistance function, using the following technical solution: A method for preparing a disease-resistant potato seed dressing agent, applied to the aforementioned disease-resistant potato seed dressing agent, includes the following steps: Raw material processing: Each raw material is pre-treated separately and then set aside for use; Capsule preparation: According to the weight parts, pretreated cationic modified chitosan was added to deionized water and stirred to obtain an aqueous solution. N-methoxy-N-o-tolyl-3-(furanyl)propionamide and eugenol polyoxyethylene ether derivative were mixed and added to the aqueous solution to form a pre-emulsion. The pre-emulsion was spray-dried to obtain microcapsule particles with a diameter of 5-10 μm. Mixed granulation: According to the weight parts, the pretreated salicylic acid glycoside, EDTA chelated zinc, polyethylene glycol-400, tert-butylhydroquinone, anhydrous sodium sulfate and capsule particles are mixed in sequence, and water is added for atomization granulation to obtain wet particles with a particle size of 0.8-1.2 mm. Drying and sieving: The wet particles are dried to a moisture content of ≤1.0%, screened by a grading sieve, and then sealed and packaged to complete the preparation.

[0012] By adopting the above technical solutions, raw material processing provides a pure and stable raw material base, microcapsule preparation realizes the protection and sustained release design of core bactericidal components, mixed granulation uniformly integrates and shapes multiple functional components, drying and sieving ensure that the product's physicochemical indicators meet the standards, and the entire process works in synergy to avoid mutual interference between active ingredients and ensure that the product composition is uniform and the dosage form is stable.

[0013] Preferably, in the raw material processing step, the pretreatment includes the following steps: N-methoxy-N-o-tolyl-3-(furanyl)propionamide, eugenol polyoxyethylene ether derivative, and salicylic acid glycoside were pulverized and then sieved through a 100-120 mesh sieve. The cationic modified chitosan was dried at 80-85℃ for 2-3 hours until the moisture content was ≤0.5%. EDTA-chelated zinc, polyethylene glycol-400, tert-butylhydroquinone, and anhydrous sodium sulfate were dried at 58-62℃ for 0.5-1.5 hours.

[0014] By adopting the above technical solutions, the pretreatment process for different raw material characteristics can effectively remove impurities, oxide scale and excess moisture from the raw materials, while ensuring the uniform particle size of the core active ingredients and avoiding the influence of moisture on microcapsule formation and ingredient mixing.

[0015] Preferably, in the capsule preparation step, the aqueous solution obtained after stirring is obtained by stirring at 50-60℃ and 300-400rpm for 15-20min to obtain an aqueous solution with a mass concentration of 5-8%; the primary emulsion is formed by stirring at 60-65℃ and 500-600rpm for 30-40min; the inlet air temperature of the spray drying is 160-170℃, the outlet air temperature is 70-80℃, the atomization pressure is 0.4-0.6MPa, and the atomized particle size is 50-100μm.

[0016] By adopting the above technical solution, the parameters of aqueous solution preparation, colostrum formation and spray drying are matched in a coordinated manner to ensure that the cationic modified chitosan is fully dissolved and the core bactericidal components are uniformly dispersed in the colostrum, forming microcapsule particles that have both good mechanical strength and can achieve slow release of active ingredients.

[0017] Preferably, in the mixing and granulation step, the mixing involves mixing the microcapsule particles with pretreated salicylic acid glycoside and EDTA chelated zinc at 800-1000 rpm for 25-30 min, adding pretreated polyethylene glycol-400 and tert-butylhydroquinone and continuing mixing for 10-15 min, and then adding pretreated anhydrous sodium sulfate and mixing for 15-20 min. The ambient temperature during mixing is 20-30℃, and the relative humidity is 40-60%. When adding water for atomized granulation, the amount of water added is 10-15% of the total mass of the mixture, the granulation extrusion pressure is 0.8-1.2 MPa, the atomization pressure of the atomized water is 0.3-0.5 MPa, and the atomized particle size is 50-100 μm.

[0018] By adopting the above technical solutions, step-by-step mixing and environmental parameter control ensure that microcapsule particles are uniformly integrated with other functional components. The parameters of atomization granulation work together to ensure that the wet particles are formed in a regular manner. Particle size control not only facilitates subsequent drying and sieving, but also improves the disintegration and dispersibility of the finished product in water, ensuring uniform adhesion to the seed surface.

[0019] Preferably, in the drying and sieving step, the drying is carried out using fluidized bed drying at a temperature of 70-75℃, an air velocity of 1.0-1.5m / s, and a drying time of 2-2.5h. The grading sieve uses a 70-80μm wet sieve and a 1.0-1.5mm dry sieve, and the particle hardness after sieving is 15-25N.

[0020] By adopting the above technical solutions, the temperature, wind speed and time parameters of fluidized bed drying are coordinated to quickly remove moisture from wet particles while avoiding particle cracking or deactivation of active ingredients. The combination of grading sieve and hardness control ensures that the finished particles have uniform particle size and compact structure.

[0021] Preferably, in the drying and sieving step, the sealed packaging is made of aluminum foil composite packaging bag, and nitrogen gas of 0.02-0.03MPa is introduced during packaging. After packaging, it is stored in an environment with a temperature of 15-25℃ and a relative humidity of ≤60%, and the storage period is ≤18 months.

[0022] By adopting the above technical solution, the combination of aluminum foil composite packaging bags and nitrogen protection effectively isolates oxygen and moisture, and in accordance with storage environment requirements, can reduce the rate of oxidative degradation of active ingredients in the finished product and improve storage stability.

[0023] Thirdly, this application provides a method for applying a potato seed dressing agent with disease resistance function, using the following technical solution: A method for applying a disease-resistant potato seed dressing agent, comprising the following steps: Mix potato seed dressing agent with deionized water at a mass ratio of 1:50-80 and stir at 300-500 rpm for 5-8 minutes to prepare a suspension. Mix the suspension with potato seeds at a mass ratio of 1:10-15, stir at 60-80 rpm for 3-5 minutes, spread it out in a cool, ventilated place for 1-2 hours to dry, and then sow.

[0024] By adopting the above technical solution, the seed dressing agent is mixed with water in a specific ratio, and the stirring speed and time are appropriate to ensure that the finished product quickly disintegrates to form a uniform suspension. The ratio of the suspension to the seeds is appropriate, and the stirring and drying steps are coordinated to ensure that each seed is evenly coated with a protective layer while avoiding excessive moisture from affecting seed germination. Therefore, the effect of uniform protection, convenient use, and no impact on seed vigor is achieved, ensuring that the seed dressing agent can fully exert its disease resistance effect.

[0025] In summary, this application has the following beneficial effects: 1. Because this application uses N-methoxy-N-o-tolyl-3-(furanyl)propamide, eugenol polyoxyethylene ether derivative, and salicylic acid glycoside to construct a multi-component core system, N-methoxy-N-o-tolyl-3-(furanyl)propamide directly acts on the key metabolic links of pathogens, plant-derived derivatives exert broad-spectrum antibacterial effects, inducible components activate the potato's own disease resistance mechanism, and cationic modified chitosan synergistically with other auxiliary components, it not only improves the adhesion stability of each active ingredient on the seed surface, but also achieves the slow release of active ingredients and maintains a long-term effective concentration. The components work together to form a three-dimensional protective system, thus achieving the effect of pathogens being difficult to develop drug resistance and the control effect being stable for a long time.

[0026] 2. In this application, EDTA chelates zinc to form a synergistic effect with disease-resistant components and modified chitosan. Zinc provides essential nutrients for potato seedlings and participates in the synthesis of disease-resistant enzymes, which not only enhances the disease resistance effect but also promotes root development and seedling growth. This avoids the inhibitory effect of high-concentration agents on seed germination. Instead, the synergistic effect of the components improves the seed germination rate and seedling fresh weight, which not only reduces disease losses but also lays a good foundation for the subsequent growth of potatoes, helping to improve the overall yield and quality.

[0027] 3. The method of this application protects the bactericidal components through the microencapsulation process of cationic modified chitosan, preventing them from being rapidly lost or degraded in the soil. At the same time, it achieves slow release of active ingredients, prolonging the effective concentration maintenance time. Furthermore, the enduring resistance induced by salicylic acid glycosides is combined with the sustained release effect of microcapsules, achieving long-term protection without the need for additional pesticide application, reducing the number of pesticide applications while ensuring healthy seedling growth. Attached Figure Description

[0028] Figure 1 This is a flowchart of a method for preparing a potato seed dressing agent with disease resistance provided in this application; Figure 2 This is a flowchart illustrating the application method of a potato seed dressing agent with disease resistance provided in this application. Detailed Implementation

[0029] The present application will be further described in detail below with reference to the accompanying drawings and embodiments.

[0030] Technical Concept: Potatoes are an important food and economic crop, and are susceptible to various seed-borne and soil-borne diseases during cultivation, affecting yield and quality. Seed treatment is a key means of controlling these diseases, but chemical seed treatment agents in related technologies mostly rely on a single bactericidal mechanism, lack synergistic design between components, and long-term use can easily lead to pathogen resistance, resulting in a gradual decline in control effectiveness. At the same time, active ingredients are easily lost or degraded in the soil, with a short effective period, which cannot cover the critical protection stage from seed germination to seedling stage.

[0031] To address the aforementioned issues, this application combines novel plant-derived amide fungicides with resistance-inducing components to form a multi-faceted mechanism of action that directly kills bacteria, inhibits bacterial growth, and activates the potato's own resistance. This effectively reduces the risk of resistance. The fungicidal components are encapsulated using a modified chitosan microcapsule process, enabling slow release of the active ingredients and extending the duration of effectiveness. Simultaneously, the raw material pretreatment, mixing, granulation, drying, and sieving processes ensure uniform dispersion of all components and stable formulation, resulting in a potato seed dressing agent that combines stable control, long-lasting protection, and growth promotion.

[0032] Unless otherwise specified, all experimental methods used below are conventional methods. All materials, reagents, methods, and instruments used, unless otherwise specified, are conventional materials, reagents, methods, and instruments in this field, which can be obtained commercially or prepared according to literature methods by those skilled in the art.

[0033] To better understand the above technical solutions, the technical solutions of the present invention will be clearly and completely described below in conjunction with embodiments.

[0034] The following is a further description with reference to the embodiments: Example 1: Please see the appendix Figure 1 Appendix Figure 2 A potato seed dressing agent with disease resistance function comprises the following raw materials in parts by weight: 10 parts N-methoxy-N-o-tolyl-3-(furanyl)propionamide, 6.5 parts eugenol polyoxyethylene ether derivative, 4 parts salicylic acid glycoside, 8 parts cationic modified chitosan, 3 parts EDTA chelated zinc, 4.5 parts polyethylene glycol-400, 0.75 parts tert-butylhydroquinone, and 63.25 parts anhydrous sodium sulfate.

[0035] The cationic modified chitosan has a degree of substitution of 1 and a molecular weight of 65,000 Da. The preparation method of the eugenol polyoxyethylene ether derivative includes the following steps: Eugenol and ethylene oxide were reacted at 130°C and 0.4 MPa for 5 h under the action of an alkaline catalyst to obtain the reactants. The molar ratio of eugenol to ethylene oxide was 1:12.5, and the amount of alkaline catalyst was 0.75% of the mass of eugenol. The reactants were purified by vacuum distillation to obtain an eugenol polyoxyethylene ether derivative with a degree of polymerization of 13.

[0036] The preparation method of N-methoxy-N-o-tolyl-3-(furanyl)propionamide includes the following steps: 3-(furanyl)propionic acid and o-toluidine were reacted at 30°C for 9 h under the catalysis of dicyclohexylcarbodiimide to obtain the catalytic reactant. The molar ratio of 3-(furanyl)propionic acid to o-toluidine was 1:1.2. A methylating agent was added to the catalytic reactants, and the reaction was continued at 45°C for 7 hours. After recrystallization and purification, N-methoxy-N-o-tolyl-3-(furanyl)propionamide was obtained. The amount of methylating agent added was 1.35 times the molar amount of o-toluidine.

[0037] A method for preparing a disease-resistant potato seed dressing agent, applied to the aforementioned disease-resistant potato seed dressing agent, includes the following steps: Raw material processing: Each raw material is pre-treated separately and then set aside for use; Capsule preparation: According to the weight parts, pretreated cationic modified chitosan was added to deionized water and stirred to obtain an aqueous solution. N-methoxy-N-o-tolyl-3-(furanyl)propionamide and eugenol polyoxyethylene ether derivative were mixed and added to the aqueous solution to form a pre-emulsion. The pre-emulsion was spray-dried to obtain microcapsule particles with a capsule diameter of 7.5 μm. Mixed granulation: According to the weight parts, the pretreated salicylic acid glycoside, EDTA chelated zinc, polyethylene glycol-400, tert-butylhydroquinone, anhydrous sodium sulfate and capsule particles are mixed in sequence, water is added and atomized to granulate, and wet particles with a particle size of 1mm are obtained. Drying and sieving: The wet particles are dried to a moisture content of ≤1.0%, screened by a grading sieve, and then sealed and packaged to complete the preparation.

[0038] In the raw material processing steps, pretreatment includes the following steps: N-methoxy-N-o-tolyl-3-(furanyl)propionamide, eugenol polyoxyethylene ether derivative, and salicylic acid glycoside were pulverized and then sieved through a 110-mesh sieve. The cationic modified chitosan was dried at 82.5℃ for 2.5 h until the moisture content was ≤0.5%. EDTA-chelated zinc, polyethylene glycol-400, tert-butylhydroquinone, and anhydrous sodium sulfate were dried at 60°C for 1 hour.

[0039] In the capsule preparation process, the aqueous solution obtained after stirring is obtained by stirring at 55℃ and 350rpm for 17.5min to obtain an aqueous solution with a mass concentration of 6.5%. The primary emulsion is formed by stirring at 62.5℃ and 550rpm for 35min. The inlet air temperature of the spray drying is 165℃, the outlet air temperature is 75℃, the atomization pressure is 0.5MPa, and the atomized particle size is 65μm.

[0040] In the mixing and granulation step, the microcapsule particles were mixed with pretreated salicylic acid glycoside and EDTA chelated zinc at 900 rpm for 27.5 min. Then, pretreated polyethylene glycol-400 and tert-butylhydroquinone were added and the mixture was continued for 12.5 min. Finally, pretreated anhydrous sodium sulfate was added and the mixture was mixed for 17.5 min. The ambient temperature during mixing was 25℃ and the relative humidity was 50%. When adding water for atomization granulation, the amount of water added was 12.5% ​​of the total mass of the mixture. The granulation extrusion pressure was 1 MPa, the atomization pressure of the atomizing water was 0.4 MPa, and the atomized particle size was 65 μm.

[0041] In the drying and screening process, fluidized bed drying is used at a temperature of 72.5℃, an air velocity of 1.25m / s, and a drying time of 2.25h. The grading screens are a 75μm wet screen and a 1.25mm dry screen, and the particle hardness after screening is 20N.

[0042] In the drying and sieving steps, the sealed packaging uses aluminum foil composite packaging bags. Nitrogen gas at 0.025MPa is introduced during packaging. After packaging, it is stored in an environment with a temperature of 20℃ and a relative humidity of ≤60%, and the storage period is ≤18 months.

[0043] A method for applying a disease-resistant potato seed dressing agent, comprising the following steps: Potato seed dressing agent was mixed with deionized water at a mass ratio of 1:65 and stirred at 400 rpm for 6.5 min to prepare a suspension. Mix the suspension with potato seeds at a mass ratio of 1:12.5, stir at 70 rpm for 4 minutes, spread them out in a cool, ventilated place at a depth of 2.5 cm and let them dry for 1.5 hours before sowing.

[0044] Example 2: This example differs from Example 1 above in that: A potato seed dressing agent with disease resistance function comprises the following raw materials in parts by weight: 12 parts of N-methoxy-N-o-tolyl-3-(furanyl)propionamide, 8 parts of eugenol polyoxyethylene ether derivative, 5 parts of salicylic acid glycoside, 10 parts of cationic modified chitosan, 4 parts of EDTA chelated zinc, 6 parts of polyethylene glycol-400, 1 part of tert-butylhydroquinone, and 54 parts of anhydrous sodium sulfate.

[0045] Example 3: This example differs from Example 1 above in that: A potato seed dressing agent with disease resistance function comprises the following raw materials in parts by weight: 8 parts of N-methoxy-N-o-tolyl-3-(furanyl)propionamide, 5 parts of eugenol polyoxyethylene ether derivative, 3 parts of salicylic acid glycoside, 6 parts of cationic modified chitosan, 2 parts of EDTA chelated zinc, 3 parts of polyethylene glycol-400, 0.5 parts of tert-butylhydroquinone, and 72.5 parts of anhydrous sodium sulfate.

[0046] Comparative Example 1: A potato seed dressing agent comprising the following raw materials in parts by weight: 15 parts metalaxyl, 5 parts fludioxonil, 5 parts polyethylene glycol-400, and 75 parts anhydrous sodium sulfate, in the form of a wettable powder.

[0047] A method for preparing a potato seed dressing agent includes the following steps: metalaxyl and fludioxonil are pulverized to 100 mesh, mixed evenly with polyethylene glycol-400 and anhydrous sodium sulfate, pulverized by air jet milling, and sieved to collect 80-100 mesh particles to obtain the finished product.

[0048] A method for using a potato seed dressing agent: Mix the finished product with water at a mass ratio of 1:50, stir for 5 minutes to prepare a suspension, mix with potato seeds at a mass ratio of 1:10, dry for 1 hour, and then sow.

[0049] Comparative Example 2: This comparative example differs from Example 1 above in that: A potato seed dressing agent with disease resistance function comprises the following raw materials in parts by weight: 10 parts N-methoxy-N-o-tolyl-3-(furanyl)propionamide, 6.5 parts eugenol polyoxyethylene ether derivative, 8 parts cationic modified chitosan, 3 parts EDTA chelated zinc, 4.5 parts polyethylene glycol-400, 0.75 parts tert-butylhydroquinone, and 67.25 parts anhydrous sodium sulfate; The rest is the same as in Example 1.

[0050] Comparative Example 3: This comparative example differs from Example 1 above in that: The weight parts of eugenol polyoxyethylene ether derivative were adjusted to 12 parts, and the weight parts of anhydrous sodium sulfate were adjusted to 59.25 parts. The rest is the same as in Example 1.

[0051] Comparative Example 4: This comparative example differs from Example 1 above in that: There is no capsule preparation step; N-methoxy-N-o-tolyl-3-(furanyl)propionamide and eugenol polyoxyethylene ether derivative are directly pulverized, sieved, and then mixed with the remaining raw materials for granulation. The rest is the same as in Example 1.

[0052] Performance testing: Efficacy against seed-borne diseases: In accordance with GB / T 17980.101-2004 "Guidelines for Field Efficacy Tests of Pesticides (I) Control of Seed-borne Diseases of Potatoes with Fungicides", field plot trials were set up, with each treatment replicated 3 times. The number of diseased plants was counted 30 days after sowing, and the efficacy was calculated. Soil-borne disease control efficacy: Calculate the control efficacy by counting the number of plants infected with soil-borne diseases 60 days after sowing according to the above standards. Germination rate: According to GB / T 3543.4-1995 "Specifications for Seed Inspection of Crops - Germination Test", 200 treated seeds were taken and cultured for 7 days at 25℃ and 70% relative humidity, and the germination rate was calculated. Fresh weight of seedlings: 45 days after sowing, 10 seedlings were randomly selected from each plot, and the fresh weight of each seedling was measured and the average value was taken. Duration of effectiveness: By regularly monitoring the disease control efficacy in the field, when the efficacy drops below 50%, record the number of days from sowing to that point in time.

[0053] Table 1

[0054] As can be seen from Examples 1-3 and Comparative Example 1, and Table 1, the synergistic design of the ternary core components and the modified chitosan microcapsule process in this application can optimize the comprehensive disease resistance effect of potato seed dressing agents; N-methoxy-N-o-tolyl-3-(furanyl)propionamide exerts a direct bactericidal effect, eugenol polyoxyethylene ether derivatives achieve broad-spectrum antibacterial activity, and salicylic acid glycosides activate the potato's own resistance, with the three mechanisms of action complementing each other; the modified chitosan microcapsules protect the core components and achieve slow release, synergistically improving the control effect of seed-borne and soil-borne diseases, extending the effective period while taking into account seed germination and seedling growth.

[0055] As can be seen from Examples 1-3 and Comparative Example 2, and Table 1, the synergistic effect of salicylic acid glycosides with other components in this application can enhance the long-term protective ability of the seed dressing agent. As an inducing resistance component, salicylic acid glycosides form a three-dimensional protective system with N-methoxy-N-o-tolyl-3-(furanyl)propionamide and eugenol polyoxyethylene ether derivatives, which activates the potato's own disease resistance genes, makes up for the lack of long-term effectiveness of single bactericidal components, ensures continuous protection from seed germination to seedling stage, and steadily improves the overall disease control efficacy.

[0056] As can be seen from Examples 1-3 and Comparative Example 3, and Table 1, the combination of raw materials in this application can ensure the synergistic effect of each component; N-methoxy-N-o-tolyl-3-(furanyl)propionamide, eugenol polyoxyethylene ether derivative, salicylic acid glycoside and other components not only ensure that each component fully performs its own function, but also avoid the performance imbalance caused by excessive or insufficient single component, so as to achieve a balanced improvement in disease resistance and growth promotion.

[0057] As can be seen from Examples 1-3 and Comparative Example 4, and Table 1, the modified chitosan microcapsule preparation process in this application can improve the stability and utilization rate of the core components; the microcapsule structure formed by modified chitosan can effectively protect the core bactericidal components from environmental factors, slow down the release rate of the components, and avoid rapid loss or degradation; at the same time, it promotes the uniform dispersion of the core components in the seed dressing agent, ensuring that each seed can obtain uniform agent coverage and ensuring the consistency of disease control.

[0058] This specific embodiment is merely an explanation of this application and is not intended to limit it. After reading this specification, those skilled in the art can make modifications to this embodiment without contributing any inventive step, but such modifications are protected by patent law as long as they fall within the scope of the claims of this application.

Claims

1. A potato seed dressing agent with disease resistance function, characterized in that, The raw materials include the following parts by weight: 8-12 parts of N-methoxy-N-o-tolyl-3-(furanyl)propionamide, 5-8 parts of eugenol polyoxyethylene ether derivative, 3-5 parts of salicylic acid glycoside, 6-10 parts of cationic modified chitosan, 2-4 parts of EDTA chelated zinc, 3-6 parts of polyethylene glycol-400, 0.5-1.0 parts of tert-butylhydroquinone, and 54-72.5 parts of anhydrous sodium sulfate.

2. The potato seed dressing agent with disease resistance function according to claim 1, characterized in that: The cationic modified chitosan has a degree of substitution of 0.8-1.2 and a molecular weight of 50,000-80,000 Da. The preparation method of the eugenol polyoxyethylene ether derivative includes the following steps: Eugenol and ethylene oxide are reacted at 120-140℃ and 0.3-0.5MPa pressure for 4-6 hours under the action of an alkaline catalyst to obtain the reactant. The molar ratio of eugenol to ethylene oxide is 1:10-15, and the amount of alkaline catalyst is 0.5-1.0% of the mass of eugenol. The reactants were purified by vacuum distillation to obtain eugenol polyoxyethylene ether derivatives with a degree of polymerization of 10-15.

3. The potato seed dressing agent with disease resistance function according to claim 1, characterized in that: The preparation method of the N-methoxy-N-o-tolyl-3-(furanyl)propionamide includes the following steps: 3-(furanyl)propionic acid and o-toluidine were reacted at 25-35°C for 8-10 h under the catalysis of dicyclohexylcarbodiimide to obtain the catalytic reactant. The molar ratio of 3-(furanyl)propionic acid to o-toluidine was 1:1.1-1.

3. A methylating agent was added to the catalytic reactants, and the reaction was continued at 40-50℃ for 6-8 hours. After recrystallization and purification, N-methoxy-N-o-tolyl-3-(furanyl)propionamide was obtained. The amount of the methylating agent added was 1.2-1.5 times the molar amount of o-toluidine.

4. A method for preparing a potato seed dressing agent with disease resistance function, characterized in that: The application of a potato seed dressing agent with disease resistance function according to any one of claims 1-3 includes the following steps: Raw material processing: Each raw material is pre-treated separately and then set aside for use; Capsule preparation: According to the weight parts, pretreated cationic modified chitosan was added to deionized water and stirred to obtain an aqueous solution. N-methoxy-N-o-tolyl-3-(furanyl)propionamide and eugenol polyoxyethylene ether derivative were mixed and added to the aqueous solution to form a pre-emulsion. The pre-emulsion was spray-dried to obtain microcapsule particles with a diameter of 5-10 μm. Mixed granulation: According to the weight parts, the pretreated salicylic acid glycoside, EDTA chelated zinc, polyethylene glycol-400, tert-butylhydroquinone, anhydrous sodium sulfate and capsule particles are mixed in sequence, and water is added for atomization granulation to obtain wet particles with a particle size of 0.8-1.2 mm. Drying and sieving: The wet particles are dried to a moisture content of ≤1.0%, screened by a grading sieve, and then sealed and packaged to complete the preparation.

5. The method for preparing a potato seed dressing agent with disease resistance function according to claim 4, characterized in that: In the raw material processing steps, the pretreatment includes the following steps: N-methoxy-N-o-tolyl-3-(furanyl)propionamide, eugenol polyoxyethylene ether derivative, and salicylic acid glycoside were pulverized and then sieved through a 100-120 mesh sieve. The cationic modified chitosan was dried at 80-85℃ for 2-3 hours until the moisture content was ≤0.5%. EDTA-chelated zinc, polyethylene glycol-400, tert-butylhydroquinone, and anhydrous sodium sulfate were dried at 58-62℃ for 0.5-1.5 hours.

6. The method for preparing a potato seed dressing agent with disease resistance function according to claim 4, characterized in that: In the capsule preparation steps, the aqueous solution obtained after stirring is obtained by stirring at 50-60℃ and 300-400rpm for 15-20min to obtain an aqueous solution with a mass concentration of 5-8%. The primary emulsion is formed by stirring at 60-65℃ and 500-600rpm for 30-40min. The inlet air temperature of the spray drying is 160-170℃, the outlet air temperature is 70-80℃, the atomization pressure is 0.4-0.6MPa, and the atomized particle size is 50-100μm.

7. The method for preparing a potato seed dressing agent with disease resistance function according to claim 4, characterized in that: In the mixing and granulation step, the mixing involves mixing the microcapsule particles with pretreated salicylic acid glycoside and EDTA chelated zinc at 800-1000 rpm for 25-30 min, adding pretreated polyethylene glycol-400 and tert-butylhydroquinone and continuing mixing for 10-15 min, and then adding pretreated anhydrous sodium sulfate and mixing for 15-20 min. The ambient temperature during mixing is 20-30℃ and the relative humidity is 40-60%. When adding water for atomization granulation, the amount of water added is 10-15% of the total mass of the mixture, the granulation extrusion pressure is 0.8-1.2 MPa, the atomization pressure of the atomizing water is 0.3-0.5 MPa, and the atomized particle size is 50-100 μm.

8. The method for preparing a potato seed dressing agent with disease resistance function according to claim 4, characterized in that: In the drying and sieving step, the drying is carried out using fluidized bed drying at a temperature of 70-75℃, an air velocity of 1.0-1.5m / s, and a drying time of 2-2.5h. The grading sieve uses a 70-80μm wet sieve and a 1.0-1.5mm dry sieve, and the hardness of the particles after sieving is 15-25N.

9. The method for preparing a potato seed dressing agent with disease resistance function according to claim 4, characterized in that: In the drying and sieving steps, the sealed packaging uses aluminum foil composite packaging bags. Nitrogen gas at 0.02-0.03 MPa is introduced during packaging. After packaging, it is stored in an environment with a temperature of 15-25℃ and a relative humidity of ≤60%, and the storage period is ≤18 months.

10. A method for applying a potato seed dressing agent with disease resistance function, characterized in that: The application of a potato seed dressing agent with disease resistance function according to any one of claims 1-3 includes the following steps: Mix potato seed dressing agent with deionized water at a mass ratio of 1:50-80 and stir at 300-500 rpm for 5-8 minutes to prepare a suspension. Mix the suspension with potato seeds at a mass ratio of 1:10-15, stir at 60-80 rpm for 3-5 minutes, spread it out in a cool, ventilated place for 1-2 hours to dry, and then sow.

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