Wheat peptide with muscle and weight gaining effect, composition containing wheat peptide as well as preparation method and application of wheat peptide

Abstract

The invention discloses a wheat peptide with muscle and weight gaining effects, a composition containing the wheat peptide as well as a preparation method and application of the wheat peptide, and relates to the technical field of food processing. The wheat peptide with the muscle and weight gaining effect is obtained by performing enzymolysis on wheat protein by adopting serine protease, trypsin and flavourzyme and then adding papain for enzymolysis. The wheat peptide can promote myoblast proliferation, improve the holding power and weight of mice, and effectively improve the relative level of IGF-1 in serum. In addition, the vitamin B6 and the vitamin B6 can cooperate to promote the absorption of whey protein.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of food processing, in particular to a wheat peptide with muscle mass and weight increasing effect, a composition containing the same, and a preparation method and application thereof. BACKGROUND

[0002] Sarcopenia, also known as muscle loss, is a kind of aging-related syndrome of skeletal muscle mass, strength or body function decline. At present, the prevention and treatment of sarcopenia is mainly through drug intervention, exercise therapy and nutritional intervention. However, the drugs for treating sarcopenia are limited, and generally hormone intervention may have certain drawbacks. Exercise therapy can increase muscle strength and muscle content to a certain extent. Nutritional intervention generally improves muscle quantity and muscle function by ingesting nutrients such as carbohydrates and proteins. Wheat protein can improve muscle quantity and muscle energy, but has solubility and absorption limitations, and the muscle increasing effect is poor. Therefore, it is important to provide a wheat peptide with good muscle increasing effect. SUMMARY

[0003] The present application aims to at least solve one of the technical problems existing in the prior art. To this end, the present application provides a preparation method of a wheat peptide.

[0004] The present application also provides a wheat peptide prepared by the above preparation method.

[0005] The present application also provides a composition comprising the above wheat peptide.

[0006] The present application also provides an application of the above wheat peptide or composition.

[0007] The present application also provides a muscle increasing powder comprising the above wheat peptide or composition.

[0008] According to the preparation method of the wheat peptide of the first aspect of the present application, the preparation method comprises: S1, preparing a first mixture of wheat protein, serine protease, trypsin and flavor protease, performing first enzymolysis to obtain a primary solution; S2, preparing a second mixture of the primary solution and papain, and performing second enzymolysis.

[0009] According to the preparation method of the present application, at least the following beneficial effects are achieved: The preparation method of the present application first uses serine protease, trypsin and flavor protease for enzymolysis treatment, and then adds papain for enzymolysis treatment, so as to prepare a wheat peptide with good muscle mass and weight increasing effect.

[0010] According to some embodiments of the application, the first mixture further comprises water.

[0011] According to some embodiments of the application, the mass volume ratio of the water to the wheat protein is 1 L: (50-200) g. For example, it can be 1 L: 50 g, 1 L: 60 g, 1 L: 70 g, 1 L: 80 g, 1 L: 90 g, 1 L: 100 g, 1 L: 110 g, 1 L: 120 g, 1 L: 130 g, 1 L: 140 g, 1 L: 150 g, 1 L: 160 g, 1 L: 170 g, 1 L: 180 g, 1 L: 190 g, or 1 L: 200 g.

[0012] According to some embodiments of the application, the method for preparing the first mixture comprises the following steps: After the wheat protein is mixed with water evenly, the serine protease, trypsin, and prolamin protease are added and mixed to obtain the first mixture.

[0013] According to some embodiments of the application, in S1, the pH of the first enzymolysis is 8-10. For example, it can be 8, 8.1, 8.2, 8.3, 8.4, 8.5, 8.6, 8.7, 8.8, 8.9, 9, 9.1, 9.2, 9.3, 9.4, 9.5, 9.6, 9.7, 9.8, 9.9, or 10.

[0014] According to some embodiments of the application, in S1, the temperature of the first enzymolysis is 40℃-50℃. For example, it can be 40℃, 41℃, 42℃, 43℃, 44℃, 45℃, 46℃, 47℃, 48℃, 49℃, or 50℃.

[0015] According to some embodiments of the application, in S1, the time of the first enzymolysis is 1 h-2 h. For example, it can be 1 h, 1.1 h, 1.2 h, 1.3 h, 1.4 h, 1.5 h, 1.6 h, 1.7 h, 1.8 h, 1.9 h, or 2 h.

[0016] According to some embodiments of the application, in S1, the stirring speed of the first enzymolysis is (40-60) rpm. For example, it can be 40 rpm, 41 rpm, 42 rpm, 43 rpm, 44 rpm, 45 rpm, 46 rpm, 47 rpm, 48 rpm, 49 rpm, 50 rpm, 51 rpm, 52 rpm, 53 rpm, 54 rpm, 55 rpm, 56 rpm, 57 rpm, 58 rpm, 59 rpm, or 60 rpm.

[0017] According to some embodiments of the application, in S2, the pH of the second enzymatic hydrolysis is 6.5-8.5. For example: it can be 6.5, 6.6, 6.7, 6.8, 6.9, 7, 7.1, 7.2, 7.3, 7.4, 7.5, 7.6, 7.7, 7.8, 7.9, 8, 8.1, 8.2, 8.3, 8.4 or 8.5.

[0018] According to some embodiments of the application, in S2, the temperature of the second enzymatic hydrolysis is 35°C-50°C. For example: it can be 35°C, 36°C, 37°C, 38°C, 39°C, 40°C, 41°C, 42°C, 43°C, 44°C, 45°C, 46°C, 47°C, 48°C, 49°C or 50°C.

[0019] According to some embodiments of the application, in S2, the time of the second enzymatic hydrolysis is 2 h-3 h. For example: it can be 2 h, 2.1 h, 2.2 h, 2.3 h, 2.4 h, 2.5 h, 2.6 h, 2.7 h, 2.8 h, 2.9 h or 3 h.

[0020] According to some embodiments of the application, in S2, the stirring speed of the first enzymatic hydrolysis is (40-60) rpm. For example: it can be 40 rpm, 41 rpm, 42 rpm, 43 rpm, 44 rpm, 45 rpm, 46 rpm, 47 rpm, 48 rpm, 49 rpm, 50 rpm, 51 rpm, 52 rpm, 53 rpm, 54 rpm, 55 rpm, 56 rpm, 57 rpm, 58 rpm, 59 rpm or 60 rpm.

[0021] According to some embodiments of the application, the mass ratio of the serine protease, trypsin, flavor protease is 1: (0.3-3.2): (0.8-7). For example: it can be 1: 3: 5, 1: 0.5: 0.83, 1: 0.33: 6.67.

[0022] According to some embodiments of the application, the mass ratio of the serine protease, trypsin, flavor protease is 1: (0.3-3.2): (4.8-7).

[0023] According to some embodiments of the application, the mass ratio of the serine protease, trypsin, flavor protease is 1: (2.8-3.2): (4.8-5.2).

[0024] According to some embodiments of the application, the mass ratio of the wheat protein, serine protease, trypsin, flavor protease is 100: (0.1-0.6): (0.3-1): (0.5-2).

[0025] According to some embodiments of the application, the mass ratio of the wheat protein, serine protease, trypsin, and flavor protease is 100: (0.1-0.3): (0.6-1): (1-2).

[0026] According to some embodiments of the application, the mass ratio of the wheat protein, serine protease, trypsin, and flavor protease is 100: (0.15-0.25): (0.55-0.65): (0.9-1.1).

[0027] According to some embodiments of the application, the mass ratio of the wheat protein and papain is 100: (1-2). For example, it can be 100: 1, 100: 1.1, 100: 1.2, 100: 1.3, 100: 1.4, 100: 1.5, 100: 1.6, 100: 1.7, 100: 1.8, 100: 1.9, or 100: 2.

[0028] According to some embodiments of the application, the preparation method further comprises an enzymatic post-treatment.

[0029] According to some embodiments of the application, the enzymatic post-treatment comprises at least one of an enzyme inactivation treatment, a filtration treatment, and a drying treatment.

[0030] According to some embodiments of the application, the enzyme inactivation treatment comprises the following steps: treating the enzymatic product after the second enzymolysis at 90-100°C for 10-30 min.

[0031] According to some embodiments of the application, the filtration treatment comprises at least one of centrifugation, pressure filtration, and plate-and-frame filtration.

[0032] According to some embodiments of the application, the drying treatment comprises spray drying.

[0033] According to some embodiments of the application, the drying treatment comprises the following steps: concentrating to a soluble solid content of greater than 20% before spray drying.

[0034] According to some embodiments of the application, the concentration comprises rotary evaporation at (70-80) °C.

[0035] A wheat peptide according to the second aspect of the embodiments of the application is prepared by the preparation method according to the first aspect of the embodiments of the application.

[0036] The wheat peptide according to the embodiments of the application has at least the following beneficial effects: The wheat peptide of the embodiment has a good muscle growth and weight gain effect, can promote myoblast proliferation, effectively promote muscle synthesis, increase weight, and improve exercise capacity.

[0037] A composition according to the third aspect of the embodiment of the present application comprises the wheat peptide according to the second aspect of the embodiment. Since the composition adopts all the technical solutions of the wheat peptide according to the above embodiment, it at least has all the beneficial effects brought by the technical solutions of the above embodiment.

[0038] According to some embodiments of the present application, the composition further comprises whey protein and vitamin B6. The wheat peptide and vitamin B6 can synergistically promote the absorption of proteins such as whey protein, thereby further improving muscle synthesis.

[0039] According to some embodiments of the present application, the composition comprises 0.1-3 parts of the wheat peptide, 33-42 parts of the whey protein, and 0.1-1 part of the vitamin B6 in terms of mass fraction.

[0040] According to some embodiments of the present application, the whey protein comprises at least one of concentrated whey protein, hydrolyzed whey protein, and isolated whey protein.

[0041] The wheat peptide according to the second aspect of the embodiment of the fourth aspect of the embodiment of the present application or the composition according to the third aspect of the embodiment is used in the preparation of a muscle growth and / or weight gain product.

[0042] According to some embodiments of the present application, the product is selected from food, dietary supplement, medicine, or additive.

[0043] A muscle growth powder according to the fifth aspect of the embodiment of the present application comprises the wheat peptide according to the second aspect of the embodiment or the composition according to the third aspect of the embodiment.

[0044] According to some embodiments of the present application, the muscle growth powder further comprises at least one of protein, carbohydrate, probiotic, vitamin, mineral element, muscle growth factor, medium-chain triglyceride, and food additive.

[0045] According to some embodiments of the present application, the protein comprises at least one of whey protein, yeast protein, soybean protein isolate, and rice protein.

[0046] According to some embodiments of the present application, the carbohydrate comprises at least one of glucose, malt dextrin, fructose, cyclic dextrin, corn starch, malto-oligosaccharide, isomaltulose, and enzymatic oat powder.

[0047] According to some embodiments of the present application, the probiotic comprises lactic acid bacteria.

[0048] According to some embodiments of the application, the lactic acid bacteria comprise at least one of Paracaseiclostridium, Caseiclostridium, Plantariclostridium, and Lactobacilluscultured.

[0049] According to some embodiments of the application, the Paracaseiclostridium comprises at least one of Paracaseiclostridium EJOZO-101, Paracaseiclostridium PC32, Paracaseiclostridium 3Q225, and Paracaseiclostridium GFP0701.

[0050] According to some embodiments of the application, the Caseiclostridium comprises Caseiclostridium CL05.

[0051] According to some embodiments of the application, the Plantariclostridium comprises at least one of Plantariclostridium TWK10 and Plantariclostridium LP28.

[0052] According to some embodiments of the application, the Lactobacillus comprises Lactobacillus delbrueckii subsp. lactis LDL557.

[0053] According to some embodiments of the application, the vitamins comprise at least one of Vitamin A, Vitamin C, Vitamin B1, Vitamin B2, Vitamin B6, Vitamin D3, Vitamin E, Vitamin K, Folic acid, and Nicotinic acid.

[0054] According to some embodiments of the application, the mineral elements comprise at least one of Calcium, Magnesium, Zinc, and Iron.

[0055] According to some embodiments of the application, the source of Calcium comprises at least one of Calcium chloride and Calcium carbonate.

[0056] According to some embodiments of the application, the source of Zinc comprises at least one of Zinc acetate, Zinc carbonate, Zinc chloride, Zinc citrate, Zinc gluconate, Zinc lactate, Zinc sulfate, and Zinc oxide.

[0057] According to some embodiments of the application, the source of Magnesium comprises at least one of Magnesium carbonate, Magnesium chloride, Magnesium citrate, Magnesium gluconate, Magnesium glycerophosphate, Magnesium lactate, Magnesium oxide, Magnesium phosphate, Magnesium sulfate, and Magnesium aspartate.

[0058] According to some embodiments of the application, the source of Iron comprises at least one of Ferric pyrophosphate, Ferric citrate, Ferrous fumarate, and Ferrous gluconate.

[0059] According to some embodiments of the application, the muscle building factors comprise at least one of Creatine, HMB (beta-hydroxy-beta-methylbutyrate), and Taurine.

[0060] According to some embodiments of the present application, the food additive comprises at least one of a coloring agent, a flavoring agent, a preservative, an emulsifier, an anti-caking agent, a flavoring agent.

[0061] According to some embodiments of the present application, the flavoring agent comprises at least one of a natural plant ingredient, a synthetic flavoring agent, a salty agent, a sweet agent, an acid agent. For example, the natural plant ingredient comprises, but is not limited to, white peach powder, green tea powder, osmanthus powder, or lemon powder. The acid agent comprises, but is not limited to, citric acid. The sweet agent comprises, but is not limited to, sucralose.

[0062] According to some embodiments of the present application, the muscle building powder comprises, in mass fraction: 20-45 parts of protein, 40-60 parts of carbohydrate, 2-4 parts of medium-chain triglyceride, 0.1-1 part of wheat peptide, 0.001-0.5 part of probiotic, 0.05-0.1 part of vitamin, 1.5-3.5 parts of mineral element, 0.05-0.2 part of muscle building factor, 0.5-3 parts of flavoring agent, 0.05-1 part of flavoring agent.

[0063] Other features and advantages of the present application will be set forth in the description that follows, and in part will be apparent from the description, or can be learned by practice of the application. DETAILED DESCRIPTION

[0064] The idea and the technical effects of the present application will be described clearly and completely in the following embodiments. It is obvious that the described embodiments are only some of the embodiments of the present application, but not all the embodiments. Based on the embodiments of the present application, other embodiments obtained by those skilled in the art without creative labor are within the protection scope of the present application.

[0065] The specific conditions not mentioned in the embodiments are carried out according to the conventional conditions or the conditions suggested by the manufacturers. The reagents or instruments not mentioned by the manufacturers are conventional products that can be purchased in the market.

[0066] In the description of the present application, if the first, the second, etc. are described, it is only for the purpose of distinguishing technical features, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of indicated technical features or the sequence of indicated technical features.

[0067] When a numerical range is disclosed herein, the range is to be construed as having been measured continuously along the range, and including each and every value within the range. Further, when a range is provided, it is intended to include the maximum and minimum values of the range, as well as every integer within the range. Additionally, multiple ranges provided herein can be combined. In other words, unless otherwise indicated, all ranges disclosed herein are to be understood to encompass any and all sub-ranges subsumed therein. It is also to be understood that, unless otherwise indicated, all measurements, values, ratings, and other specifications provided herein are to be interpreted as being modified in all instances by "about," even if the term does not expressly appear. Furthermore, unless otherwise indicated, the use of "or" is to be interpreted as inclusive so that figure includes single options and also combinations of options.

[0068] "Quality parts" refers to a basic unit of measurement that represents the mass ratio relationship of multiple components. One part can represent any unit of mass, such as 1 g, 2.689 g, etc. If we say that the mass of component A is a parts and the mass of component B is b parts, it means that the ratio of the mass of component A to the mass of component B is a:b. Alternatively, it means that the mass of component A is aK and the mass of component B is bK (K is an arbitrary number representing a multiple factor). It should not be misunderstood that, unlike the mass fraction, the sum of the mass of all components is not limited to 100 parts.

[0069] "and / or" is used to indicate that one or both of the described situations can occur, for example, A and / or B includes (A and B) and (A or B).

[0070] The serine protease, papain, was purchased from Angel Biotechnology Co., Ltd. The trypsin was purchased from Jiangsu Ruide Bioengineering Co., Ltd. The flavor protease was purchased from Novozymes. The wheat protein was wheat gluten, which was purchased from Shandong Tongsheng Food Ingredients Co., Ltd.

[0071] Unless otherwise specified, the addition amount of serine protease, trypsin, flavor protease, and papain is relative to the wheat protein. For example, the addition amount of serine protease is 0.2 wt%, that is, 0.2 g of serine protease is added per 100 g of wheat protein.

[0072] Example 1 This example provides a wheat peptide, and the preparation method steps are as follows: S1, according to the volume-mass ratio of 1 L: 100 g, deionized water and wheat protein were added to the enzyme hydrolysis reactor, and after stirring at 60 rpm for 2 h, the pH was adjusted to 9, and serine protease 0.2 wt%, trypsin 0.6 wt%, and flavor protease 1 wt% were added. Hydrolysis was carried out at 45°C and 40 rpm for 1 h to obtain a crude hydrolysate.

[0073] S2, adjust the pH to 7, add papain 2 wt% to the crude hydrolysate, and hydrolyze at pH 7, 40°C, 50 rpm for 2 h to obtain an enzyme hydrolysate.

[0074] S3, after heating to 95℃ for 15 min to inactivate the enzyme, plate and frame filtration was performed, the filtrate was collected, and rotary evaporation was performed at 70℃ to concentrate the filtrate to a soluble solid content of greater than 20%; the concentrated solution was subjected to spray drying to obtain the wheat peptide.

[0075] Example 2 The present example provides a wheat peptide, and the preparation method steps thereof are as follows: S1, deionized water and wheat protein were added to an enzymatic reaction tank at a volume-to-mass ratio of 1 L: 100 g, stirring was performed at 60 rpm for 0.5 h, the pH was adjusted to 10, 0.6wt% of serine protease, 0.3wt% of trypsin, and 0.5wt% of flavor protease were added, and hydrolysis was performed at 40℃ and 60 rpm for 1 h to obtain a crude hydrolysate.

[0076] S2, the pH was adjusted to 6.8, 1.1wt% of papain was added to the crude hydrolysate, and hydrolysis was performed at pH 6.8, 35℃, and 50 rpm for 2 h to obtain an enzymatic hydrolysate.

[0077] S3, after heating to 95℃ for 15 min to inactivate the enzyme, plate and frame filtration was performed, the filtrate was collected, and rotary evaporation was performed at 70℃ to concentrate the filtrate to a soluble solid content of greater than 20%; the concentrated solution was subjected to spray drying to obtain the wheat peptide.

[0078] Example 3 The present example provides a wheat peptide, and the preparation method steps thereof are as follows: S1, deionized water and wheat protein were added to an enzymatic reaction tank at a volume-to-mass ratio of 1 L: 100 g, stirring was performed at 60 rpm for 0.5 h, the pH was adjusted to 10, 0.6wt% of serine protease, 0.3wt% of trypsin, and 0.5wt% of flavor protease were added, and hydrolysis was performed at 40℃ and 60 rpm for 1 h to obtain a crude hydrolysate.

[0079] S2, the pH was adjusted to 6.8, 1.1wt% of papain was added to the crude hydrolysate, and hydrolysis was performed at pH 6.8, 35℃, and 50 rpm for 2 h to obtain an enzymatic hydrolysate.

[0080] S3, after heating to 95℃ for 15 min to inactivate the enzyme, plate and frame filtration was performed, the filtrate was collected, and rotary evaporation was performed at 70℃ to concentrate the filtrate to a soluble solid content of greater than 20%; the concentrated solution was subjected to spray drying to obtain the wheat peptide.

[0081] Example 4 The present example provides a composition, which is composed of 2 parts of the wheat peptide prepared in Example 1, 37 parts of whey protein, and 0.5 parts of vitamin B6.

[0082] The preparation method of the composition is as follows: Mixing wheat peptide, whey protein and vitamin B6 evenly, then the product is obtained.

[0083] Comparative Example 1 This example provides a wheat peptide, and the preparation method is basically the same as that of Example 1, the only difference is that papain is used for enzymolysis first, and then serine protease, trypsin and flavor protease are used for enzymolysis. The steps are as follows: S1, according to the volume quality ratio 1 L: 100 g, deionized water and wheat protein are added to the enzymolysis reaction tank, after stirring at 60 rpm for 2 h, the pH is adjusted to 7, 2wt% papain is added, and hydrolysis is carried out at pH 7, 40℃ and 50 rpm for 2 h, to obtain a crude solution.

[0084] S2, adjust the pH to 9, add 0.2wt% serine protease, 0.6wt% trypsin and 1wt% flavor protease to the crude solution, hydrolyze at 45℃ and 40 rpm for 1 h, to obtain an enzymatic solution.

[0085] S3, after heating to 95℃ for 15 min to inactivate the enzyme, plate and frame filtration is carried out, the filtrate is collected, and rotary evaporation is carried out at 70℃ to concentrate to a soluble solid content of more than 20%, and then the concentrated solution is spray dried to obtain the wheat peptide.

[0086] Comparative Example 2 This example provides a wheat peptide, and the preparation method is basically the same as that of Example 1, the only difference is that serine protease, trypsin, flavor protease and papain are added at the same time for enzymolysis. The steps are as follows: S1, according to the volume quality ratio 1 L: 100 g, deionized water and wheat protein are added to the enzymolysis reaction tank, after stirring at 60 rpm for 2 h, the pH is adjusted to 8, 0.2wt% serine protease, 0.6wt% trypsin, 1wt% flavor protease and 2wt% papain are added, and hydrolysis is carried out at 43℃ and 45 rpm for 3 h, to obtain an enzymatic solution.

[0087] S2, after heating to 95℃ for 15 min to inactivate the enzyme, plate and frame filtration is carried out, the filtrate is collected, and rotary evaporation is carried out at 70℃ to concentrate to a soluble solid content of more than 20%, and then the concentrated solution is spray dried to obtain the wheat peptide.

[0088] Comparative Example 3 This example provides a wheat peptide, and the preparation method is basically the same as that of Example 1, the only difference is that papain is not used for enzymolysis. The steps are as follows: S1, 1 L: 100 g of deionized water and wheat protein were added to the enzymatic reaction tank according to the volume-mass ratio, and stirred at 60 rpm for 2 h, then the pH was adjusted to 9, 0.2wt% serine protease, 0.6wt% trypsin and 1wt% flavor protease were added, and hydrolysis was carried out at 45℃, 40 rpm for 1 h to obtain a crude hydrolysate; S2, the pH was adjusted to 7, and hydrolysis was carried out at pH 7, 40℃, 50 rpm for 2 h to obtain an enzymatic hydrolysate.

[0089] S3, after heating to 95℃ for 15 min to inactivate the enzyme, plate and frame filtration was performed, the filtrate was collected, and rotary evaporation was performed at 70℃ to concentrate the soluble solid content to more than 20%, and the concentrated solution was spray dried to obtain the wheat peptide.

[0090] Comparative Example 4 This example provides a wheat peptide, and the preparation method is basically the same as that of Example 1, and the only difference is that serine protease, trypsin and flavor protease are not used for enzymolysis. The steps are as follows: S1, 1 L: 100 g of deionized water and wheat protein were added to the enzymatic reaction tank according to the volume-mass ratio, and stirred at 60 rpm for 2 h, then the pH was adjusted to 7, 2wt% papain was added, and hydrolysis was carried out at pH 7, 40℃, 50 rpm for 2 h to obtain an enzymatic hydrolysate.

[0091] S2, after heating to 95℃ for 15 min to inactivate the enzyme, plate and frame filtration was performed, the filtrate was collected, and rotary evaporation was performed at 70℃ to concentrate the soluble solid content to more than 20%, and the concentrated solution was spray dried to obtain the wheat peptide.

[0092] Comparative Example 5 This example provides a composition, which is basically the same as Example 4, and the only difference is that vitamin B6 is lacking.

[0093] The composition consists of 2 parts of wheat peptide prepared in Example 1 and 37 parts of whey protein by mass fraction.

[0094] The preparation method of the composition is as follows: The wheat peptide and whey protein were mixed uniformly to obtain the composition.

[0095] Comparative Example 6 This example provides a composition, which is basically the same as Example 4, and the only difference is that the wheat peptide is lacking.

[0096] The composition consists of 37 parts of whey protein and 0.5 parts of vitamin B6 by mass fraction.

[0097] The preparation method of the composition is as follows: The whey protein and vitamin B6 were mixed uniformly to obtain the composition.

[0098] Comparative Example 7 This example provides a composition which is substantially the same as Example 4, except that the wheat peptide of Example 1 is replaced by an equal mass of the wheat peptide of Comparative Example 2.

[0099] The composition consists of 2 parts of the wheat peptide prepared in Comparative Example 2, 37 parts of whey protein, and 0.5 parts of vitamin B6, in mass parts.

[0100] The composition is prepared as follows: Mix the wheat peptide, whey protein, and vitamin B6 uniformly, and you get it.

[0101] Application Example 1 This example provides a muscle building powder, which consists of the following in mass parts: Concentrated whey protein powder 37 parts, edible glucose 27 parts, oligomaltose 15 parts, hydrolyzed whey protein powder 0.2 parts, enzymatic oat flour 1.2 parts, separated whey protein powder 0.2 parts, isomaltulose 2 parts, wheat peptide prepared in Example 1 0.65 parts, inactivated complex lactic acid bacteria solid beverage 0.1 parts, watermelon juice powder solid beverage 0.5 parts, medium-chain triglyceride microcapsule powder 3 parts, calcium carbonate 1.8 parts, magnesium sulfate 0.9 parts, taurine 0.12 parts, vitamin C 0.05 parts, zinc citrate 0.1 parts, niacin 0.03 parts, vitamin B1 0.002 parts, vitamin B6 0.002 parts, sucralose 0.025 parts, and edible flavoring 0.121 parts.

[0102] Among them, the inactivated lactic acid bacteria solid beverage is obtained by mixing 97 parts of oligomaltose, 1 part of inactivated plant lactobacillus TWK10 (purchased from Shenghe Biotechnology (Yangzhou) Co., Ltd.), 1 part of inactivated plant lactobacillus LP28 (purchased from Shenghe Biotechnology (Yangzhou) Co., Ltd.), and 1 part of inactivated lactobacillus delbrueckii subsp. lactis LDL557 (purchased from Shenghe Biotechnology (Yangzhou) Co., Ltd.).

[0103] Test Example 1 Myoblasts can increase the number of cells by proliferation, and eventually differentiate into myofibers to form different muscle groups, thereby increasing muscle mass.

[0104] After culturing C2C12 cells to the logarithmic phase, 1×10 3 cells / well were inoculated into a 96-well plate and cultured overnight at 37°C, 5% CO2. Each group was treated as follows: (1) Control group: replace the culture solution with 100 mL of complete culture medium (DMEM medium containing 10% fetal bovine serum, 100 U / mL penicillin, and 100 μg / mL streptomycin, same below); (2) Example 1 group: replace the culture solution with 100 μL of medium A; medium A is complete medium containing 1 mg / mL of wheat peptide of Example 1; (3) Example 2 group: replace the culture solution with 100 μL of medium B; medium B is complete medium containing 1 mg / mL of wheat peptide of Example 2; (4) Example 3 group: replace the culture solution with 100 μL of medium C; medium C is complete medium containing 1 mg / mL of wheat peptide of Example 3; (5) Comparative Example 1 group: replace the culture solution with 100 μL of medium D; medium D is complete medium containing 1 mg / mL of wheat peptide of Comparative Example 1; (6) Comparative Example 2 group: replace the culture solution with 100 μL of medium E; medium E is complete medium containing 1 mg / mL of wheat peptide of Comparative Example 2; (7) Comparative Example 3 group: replace the culture solution with 100 μL of medium F; medium F is complete medium containing 1 mg / mL of wheat peptide of Comparative Example 3; (8) Comparative Example 4 group: replace the culture solution with 100 μL of medium G; medium G is complete medium containing 1 mg / mL of wheat peptide of Comparative Example 4.

[0105] After 24 h of culture at 37℃, 5% CO2, the cell survival was detected by MTT cell proliferation and cytotoxicity detection kit (product number: C0009S, Shanghai Biyun Tian Biotechnology Co., Ltd.), and the steps were strictly followed the instructions. The absorbance (A570) of each group at 570 nm was recorded. 570 The wells without cells were used as blank group. The relative cell proliferation rate was calculated (relative cell proliferation rate (%) = (treated group A 570 - blank group A 570 ) / (control group A 570 - blank group A 570 ) - 1) x 100). Three biological replicates were set for each group.

[0106] Table 1

[0107] As shown in Table 1, the wheat peptides prepared in Examples 1-3 can significantly promote the proliferation of myoblasts; among them, the wheat peptide of Example 1 has the best promotion effect. While the wheat peptides of Comparative Examples 1-4 can also promote the proliferation of myoblasts to some extent, but the promotion effect is significantly worse than that of Example 1.

[0108] Test Example 2 After 80 SPF C57BL / 6J mice (male, 18 g-22 g) were adaptively fed for one week, they were randomly divided into 8 groups, 10 in each group. The initial body weight of each group of mice was recorded, and each group was treated as follows: (1) Control group: physiological saline was gavaged at 100 mg / kg·bw per day, and gavaged continuously for four weeks.

[0109] (2) Example 1 group: wheat peptides of Example 1 were gavaged at 100 mg / kg·bw per day, and gavaged continuously for four weeks.

[0110] (3) Example 2 group: wheat peptides of Example 2 were gavaged at 100 mg / kg·bw per day, and gavaged continuously for four weeks.

[0111] (4) Example 3 group: wheat peptides of Example 3 were gavaged at 100 mg / kg·bw per day, and gavaged continuously for four weeks.

[0112] (5) Comparative Example 1 group: wheat peptides of Comparative Example 1 were gavaged at 100 mg / kg·bw per day, and gavaged continuously for four weeks.

[0113] (6) Comparative Example 2 group: wheat peptides of Comparative Example 2 were gavaged at 100 mg / kg·bw per day, and gavaged continuously for four weeks.

[0114] (7) Comparative Example 3 group: wheat peptides of Comparative Example 3 were gavaged at 100 mg / kg·bw per day, and gavaged continuously for four weeks.

[0115] (8) Comparative Example 4 group: wheat peptides of Comparative Example 4 were gavaged at 100 mg / kg·bw per day, and gavaged continuously for four weeks.

[0116] During the experiment, the mice were free to eat and drink, and were under 12 / 12 light-dark cycle. The body weight of the mice was measured once a week. On the 29th day, the forelimb grip strength of the mice in each group was measured using a mouse grip strength tester, and the grip strength improvement rate (relative grip strength improvement rate (%) = (treatment group forelimb grip strength after experiment / control group forelimb grip strength after experiment - 1) x 100%) and body weight change rate (body weight change rate (%) = experimental body weight / experimental pre-treatment body weight x 100%) were calculated. Serum samples were collected from each group of mice, and the IGF-1 content (IGF-1 can inhibit protein degradation and promote muscle growth, and plays an important role in skeletal muscle regeneration) was detected, and the relative level of IGF-1 (IGF-1 relative level = treatment group IGF-1 content / control group IGF-1 content) was calculated.

[0117] Table 2

[0118] As shown in Table 2. The wheat peptides prepared in Examples 1-3 can significantly improve the forelimb grip strength, body weight growth rate and IGF-1 content in serum of mice. The effects of Comparative Examples 1-4 are significantly worse than those of the wheat peptides of Examples 1-3.

[0119] Test Example 3 After 60 SPF Kunming mice (male, 18 g-22 g) were adaptively fed for one week, they were randomly divided into 6 groups, 10 mice in each group. The mice were fasted at 10 o'clock the previous day, and the next morning at 8:00, the mice in each group were treated by gavage as follows, and the blood of each mouse was collected after 1 hour, the plasma was separated, and the leucine concentration in the plasma was detected.

[0120] (1) Example 4 group: 79 mg of the composition of Example 4 was dissolved in 1 mL of water, filtered and sterilized, and then gavaged into mice at 0.5 mL per mouse.

[0121] (2) Comparative Example 5 group: 78 mg of the composition of Comparative Example 5 was dissolved in 1 mL of water, filtered and sterilized, and then gavaged into mice at 0.5 mL per mouse.

[0122] (3) Comparative Example 6 group: 75 mg of the composition of Comparative Example 6 was dissolved in 1 mL of water, filtered and sterilized, and then gavaged into mice at 0.5 mL per mouse.

[0123] (4) Comparative Example 7 group: 79 mg of the composition of Comparative Example 7 was dissolved in 1 mL of water, filtered and sterilized, and then gavaged into mice at 0.5 mL per mouse.

[0124] (5) Control group: 74 mg of whey protein was dissolved in 1 mL of water, filtered and sterilized, and then gavaged into mice at 0.5 mL per mouse.

[0125] (6) Blank group: filtered and sterilized water was gavaged into mice at 0.5 mL per mouse.

[0126] Table 3

[0127] As shown in Table 3. Whey protein contains a large amount of leucine, and the composition of Example 4 can significantly promote the absorption of whey protein and rapidly increase the leucine concentration in the plasma. Compared with the control group, it can increase the leucine concentration in the plasma by about 87.66%. If the wheat peptide of Example 1, vitamin B6 or the wheat peptide of Comparative Example 2 is replaced by the wheat peptide of Comparative Example 2, the absorption-promoting effect will be significantly worse. The wheat peptide of the present application can synergistically promote the absorption of whey protein with vitamin B6.

[0128] Test Example 4 The acute oral toxicity test of the muscle building powder of application example 1 was tested according to GB 15193.3-2014 Food safety national standard Acute oral toxicity test. The steps are as follows: Ten SPF level SD rats (half male and half female) were fasted overnight with free access to water. 4000 mg of the muscle building powder of application example 1 was mixed with an appropriate amount of distilled water to obtain a test substance. Within 24 hours, the rats were gavaged with the test substance in three times at 20 mL / kg·bw, with an interval of 6 hours each time, and the total dose was 12000 mg / kg·bw. During the test, the mice were free to eat and drink, and were under 12 / 12 light-dark cycle. Continuous observation was carried out for 14 days, the death condition was recorded, and the state of the animals was observed. After the end of the test, the animals were sacrificed for gross pathology examination.

[0129] After administration of the test substance, all animals survived and were in good condition after 14 days of continuous observation, and no abnormal symptoms were found, and no abnormalities were found in each organ.

[0130] Test example 5 Thirty-six volunteers (male, 18-30 years old) without exercise habits in the past six months were recruited as subjects (exclusion criteria: having clear exercise contraindications or other diseases affecting exercise programs, adhering to a vegetarian diet, and being allergic to whey protein and casein protein). All subjects signed an informed consent form before the experiment. Nutritional supplements such as protein powder, muscle-building powder, or vitamins and hormone drugs were avoided three months before the experiment; other nutritional supplements such as protein powder, muscle-building powder, or vitamins and hormone drugs were avoided during the experiment. The subjects were randomly divided into two groups, with 18 people in each group. The subjects in the experimental group supplemented 45 g of muscle-building powder of Application Example 1 before or immediately after each of the three resistance training sessions per week. The difference between the control group and the experimental group is that the muscle-building powder is replaced with an equal amount of maltodextrin. All subjects underwent an 8-week resistance training intervention (training program as shown in Table 4), 3 times a week. The training content includes lower body exercise movements (such as deep squats, leg presses, and knee joint flexion and extension) and upper body exercise movements (such as bench press, seated row, pull-down, and dumbbell bicep curl). All movements were performed under the guidance of professional trainers to ensure standardization and correctness of the movements. Before the intervention began, the subjects underwent a 1RM test to determine the individual's initial training intensity. In weeks 1-4, 3 sets of 8-12 RM training were performed for each movement, with 2-3 minutes of rest between sets. In weeks 4-8, 4 sets of 6-10 RM training were performed for the same movements, with 1-2 minutes of rest between sets. Every 2 weeks, professional trainers adjusted the exercise load according to the updated 1RM test results to ensure the progressive and challenging nature of the training. During the study, all subjects maintained their daily activities unchanged (RM (maximum number of repetitions), i.e., in one movement, the subject can complete a certain weight of the maximum number of times in a standard manner without assistance. 1RM: the maximum weight that the subject can complete in a standard manner only once. 8-12 RM: the weight that the subject can complete in a standard manner at most 8 to 12 times).

[0131] Table 4

[0132] Table 5

[0133] Note: The data in the table are body composition, girth, deep squat, bench press, and knee joint peak torque data before and after the intervention, and the data are expressed as mean values; the change rate is ((change amount of the experimental group / change amount of the control group)-1) x 100%.

[0134] As shown in Table 5. After taking the muscle building powder of application example 1 for 8 weeks, the body weight is increased by 1561.11%, the muscle mass is increased by 647.27%, the upper arm circumference is increased by 82.67%, the hip circumference is increased by 278.10%, the chest circumference is increased by 83.04%, the deep squat is increased by 27.07%, the bench press is increased by 70.38%, the peak torque of extension muscle is increased by 47.57%, and the peak torque of flexor muscle is increased by 84.79%. In summary, the muscle building powder of application example 1 can significantly increase the body weight, increase the muscle, improve the sports ability, and quickly recover the physical strength.

[0135] The above embodiments of the present application are described in detail in combination with the examples, but the present application is not limited to the above examples, and various changes can be made within the knowledge range possessed by those skilled in the art without departing from the purpose of the present application.

Claims

1. A method for preparing wheat peptides, characterized in that, include: S1. Prepare a first mixture of wheat protein, serine protease, trypsin, and flavor protease, and perform the first enzymatic hydrolysis to obtain the initial hydrolysate; S2. Prepare a second mixture of the initial hydrolysate and papain, and perform a second enzymatic hydrolysis.

2. The preparation method according to claim 1, characterized in that, In S1, the pH of the first enzymatic hydrolysis is 8-10; And / or, the temperature of the first enzymatic hydrolysis is 40℃-50℃; And / or, the first enzymatic hydrolysis time is 1 h-2 h.

3. The preparation method according to claim 1, characterized in that, In S2, the pH of the second enzymatic hydrolysis is 6.5-8.5; And / or, the temperature of the second enzymatic hydrolysis is 35℃-50℃; And / or, the second enzymatic hydrolysis time is 2 h-3 h.

4. The preparation method according to claim 1, characterized in that, The mass ratio of wheat protein, serine protease, trypsin, and flavor protease is 100: (0.1-0.6): (0.3-1): (0.5-2).

5. The preparation method according to claim 1, characterized in that, The mass ratio of wheat protein to papain is 100:(1-2).

6. A wheat peptide, characterized in that, It is prepared by the preparation method described in any one of claims 1 to 5.

7. A composition, characterized in that, The composition includes the wheat peptide of claim 6; optionally, the composition also includes whey protein and vitamin B6.

8. The use of the wheat peptide of claim 6 or the composition of claim 7 in the preparation of products for muscle gain and / or weight gain.

9. A muscle-building powder, characterized in that, Includes the wheat peptide of claim 6 or the composition of claim 7.

10. The muscle-building powder according to claim 9, characterized in that, The muscle-building powder also includes at least one of the following: protein, carbohydrates, probiotics, vitamins, minerals, muscle-building factors, medium-chain triglycerides, and food additives.

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