Children formula food capable of improving visual function as well as preparation method and application of children formula food

Abstract

The invention provides a children formula food capable of improving visual function and a preparation method and application thereof, the children formula food contains calcium and galactose, and the mass ratio of calcium to galactose is 1: (1-18); wherein in terms of dry matter of the infant formula food, the total protein content is 7.8 g to 30 g / 100 g, the fat content is 10 g to 35 g / 100 g, and the carbohydrate content is 37.5 g to 79.3 g / 100 g. The children formula food disclosed by the invention is beneficial to improving the visual function.

Description

Technical Field

[0001] This invention relates to the field of infant and children's food technology, specifically to a pediatric formula food and its preparation method and application. Background Technology

[0002] Visual function is affected by a variety of factors, including but not limited to nutritional imbalances (such as insufficient calcium and fermentable carbohydrates), oxidative stress and inflammation, retinal / choroidal microcirculation and energy metabolism disorders, decreased synaptic plasticity in the visual pathway, excessive screen exposure and eye strain, and exposure to environmental toxins. These factors can affect the energy supply to photoreceptors, Ca²⁺, and other nutrients. + Related signal transduction and synaptic transmission, neurotrophic factors and antioxidant defense processes lead to a decrease in visual performance such as contrast sensitivity, dark adaptation and glare tolerance.

[0003] Existing research and application pathways mostly revolve around a single nutrient or a single pathway: for example, supplementing with minerals (such as calcium) or consuming dietary fiber to block / reduce the absorption of heavy metals and promote their excretion, or improving stress through antioxidants; such interventions still have limitations in terms of taste, compliance, applicable populations and broad application scenarios, and their improvement on visual function is mostly limited and conditional. Summary of the Invention

[0004] One object of the present invention is to provide an infant formula food that can improve visual function.

[0005] Another object of the present invention is to provide a method for preparing the infant formula food.

[0006] Another object of the present invention is to provide the application of the aforementioned infant formula food.

[0007] On the one hand, the present invention first provides an infant formula food containing calcium and galactose, wherein the mass ratio of calcium to galactose is 1:(1-18).

[0008] The total protein content of the infant formula, calculated by dry matter, is 7.8g-30g / 100g, the fat content is 10g-35g / 100g, and the carbohydrate content is 37.5g-79.3g / 100g.

[0009] According to some specific embodiments of the present invention, in the infant formula of the present invention, the mass ratio of calcium to galactose is 1:(2-16).

[0010] According to some specific embodiments of the present invention, in the infant formula of the present invention, the mass ratio of calcium to galactose is 1:(1-10).

[0011] According to some specific embodiments of the present invention, in the infant formula of the present invention, the mass ratio of calcium to galactose is 1:(1-6).

[0012] According to some specific embodiments of the present invention, in the infant formula of the present invention, the mass ratio of calcium to galactose is 1:(2-3).

[0013] According to some specific embodiments of the present invention, in the infant formula of the present invention, the mass ratio of calcium to galactose is 1:(9-16).

[0014] According to some specific embodiments of the present invention, in the infant formula of the present invention, the mass ratio of calcium to galactose is 1:(9-15).

[0015] According to some specific embodiments of the present invention, the infant formula food of the present invention may further include galactooligosaccharides, wherein the mass ratio of galactooligosaccharides to calcium is (9-28):1.

[0016] According to some specific embodiments of the present invention, in the infant formula of the present invention, the mass ratio of galactooligosaccharides to calcium is (15-28):1.

[0017] According to some specific embodiments of the present invention, in the infant formula of the present invention, the mass ratio of galactooligosaccharides to calcium is (18-28):1.

[0018] According to some specific embodiments of the present invention, in the infant formula food of the present invention, the mass ratio of calcium to galactose and galactooligosaccharides is 1:(2-3):(9-28).

[0019] According to some specific embodiments of the present invention, in the infant formula food of the present invention, the mass ratio of calcium to galactose and galactooligosaccharides is 1:(9-15):(9-28).

[0020] According to some specific embodiments of the present invention, in the infant formula food of the present invention, the mass ratio of calcium to galactose and galactooligosaccharides is 1:(2-3):(18-28).

[0021] According to a specific embodiment of the present invention, the raw materials for providing calcium in the infant formula of the present invention may include one or more of the following: milk calcium, calcium carbonate, calcium acetate, casein calcium, calcium chloride, calcium citrate, calcium citrate, calcium malate, calcium gluconate, calcium lactate, calcium malate, calcium hydrogen phosphate, calcium dihydrogen phosphate, calcium phosphate, calcium sulfate, calcium ascorbate, and calcium glycerophosphate.

[0022] According to a specific embodiment of the present invention, the galactose in the infant formula of the present invention is D-galactose. D-galactose is commercially available and can be obtained from exogenous or endogenous enzymatic hydrolysis of lactose. For individuals with good lactose digestion, it can be derived from equimolar amounts of lactose (i.e., galactose produced by the breakdown of lactose by endogenous lactase in the body after lactose ingestion), or from commercially available galacto-oligosaccharide raw materials, or from processed or unprocessed foods containing galactose polymers.

[0023] According to a specific embodiment of the present invention, in the infant formula of the present invention, the galactooligosaccharide can be generated by online enzymatic hydrolysis of lactose or can be derived from commercially available galactooligosaccharides.

[0024] According to a specific embodiment of the present invention, the infant formula of the present invention contains 130-1800 mg of calcium per 100g of dry matter and 0.2-32.5g of galactose per 100g of dry matter.

[0025] According to some specific embodiments of the present invention, the amount of calcium contained in the infant formula of the present invention is 130-200mg / 100g dry matter, 200-500mg / 100g dry matter, 500-800mg / 100g dry matter, 800-1000mg / 100g dry matter, 1000-1500mg / 100g dry matter, or 1500-1800mg / 100g dry matter.

[0026] According to some specific embodiments of the present invention, the galactose content in the infant formula of the present invention is 0.2-25 g / 100 g of dry matter. For example, the galactose content in the infant formula can be 0.2-1 g / 100 g of dry matter, 1-2 g / 100 g of dry matter, 2-15 g / 100 g of dry matter, or 15-25 g / 100 g of dry matter. Alternatively, the food can be prepared based on a galactose energy contribution ratio of 0.1%-10%, preferably 0.15%-4%, more preferably 0.15%-2% or 0.2%-1%.

[0027] According to a specific embodiment of the present invention, the amount of galacto-oligosaccharides in the infant formula of the present invention is 0-25g / 100g of dry matter, preferably 0-11g / 100g of dry matter. The mass ratio of galacto-oligosaccharides to galactose can be (9-28):(2-3), preferably (4-12):1. Alternatively, the food can be prepared according to the proportion of galacto-oligosaccharides in the dietary fiber of the food being 1%-50%, preferably 10%-20%, 20%-30%, 30%-40%, or 40%-45%.

[0028] In some specific embodiments of the present invention, the infant formula food further includes linoleic acid and α-linolenic acid, with the linoleic acid content being 2700-6000 mg / 100g and the α-linolenic acid content being 270-600 mg / 100g based on the dry matter content of the infant formula food.

[0029] In some specific embodiments of the present invention, the infant formula food is powdered infant formula food, liquid infant formula milk, or powdered children's modified milk powder. The powdered infant formula food includes powdered infant formula food, powdered follow-up formula food, and powdered toddler formula food. The liquid infant formula milk includes liquid infant formula milk, liquid follow-up formula milk, and liquid toddler formula milk.

[0030] According to specific embodiments of the present invention, the infant formula food further includes conventional components of infant formula or children's modified milk powder, such as one or more of nutrients, dietary fiber, inositol, L-carnitine, eicosapentaenoic acid (EPA), lutein, and nucleotides. Preferably, it includes a compound nutrient packet. In some preferred embodiments, the infant formula food or children's modified milk powder includes 0-20 parts of nutrients, which include vitamins and minerals, or further selectively include dietary fiber (fructooligosaccharides, galactooligosaccharides, polyfructose, human milk oligosaccharides, etc.), inositol, L-carnitine, docosahexaenoic acid (DHA), EPA, lutein, nucleotides, and one or more of Bifidobacterium animalis subsp. lactis. The main components of the compound nutrients include: compound vitamins: vitamin A, vitamin D, vitamin E, vitamin K1, vitamin B1, vitamin B2, vitamin B6, vitamin B12, niacin, folic acid, pantothenic acid, vitamin C, and biotin; compound minerals: sodium, potassium, calcium, phosphorus, copper, iron, zinc, manganese, iodine, selenium, magnesium, potassium, and their derivatives. The source, content, and dosage of each vitamin and mineral should comply with national standards and relevant regulations for infant formula and modified milk powder.

[0031] In some specific embodiments of the present invention, the raw material composition of the powdered infant formula food, based on the preparation of 1000 parts by weight, includes: 0-7000 parts by weight of raw milk, 0-600 parts by weight of demineralized whey powder, 0-500 parts by weight of lactose, 0-600 parts by weight of skim milk powder, 0-400 parts by weight of edible vegetable blended oil, 0-100 parts by weight of prebiotics, 0-120 parts by weight of whey protein powder, 0-20 parts by weight of phospholipids, 0-20 parts by weight of vitamins, 0-40 parts by weight of casein, and 0-30 parts by weight of minerals.

[0032] In some specific embodiments of the present invention, to prepare 1000 parts by weight of the infant formula liquid milk, the raw material composition of the infant formula liquid milk includes: 0-7000 parts by weight of raw milk, 0-500 parts by weight of skim milk powder, 0-500 parts by weight of demineralized whey powder, 0-500 parts by weight of lactose, 0-400 parts by weight of edible vegetable blended oil, 0-100 parts by weight of prebiotics, 0-120 parts by weight of whey protein powder, 0-10 parts by weight of phospholipids, 0-40 parts by weight of casein, 0-20 parts by weight of vitamins, and 0-30 parts by weight of minerals.

[0033] In some specific embodiments of the present invention, for preparing 1000 parts by weight of the powdered infant formula milk powder, the raw material composition of the powdered infant formula milk powder includes: 0-7000 parts by weight of raw milk, 0-500 parts by weight of skim milk powder, 0-600 parts by weight of demineralized whey powder, 0-120 parts by weight of whey protein powder, 0-500 parts by weight of lactose, 0-50 parts by weight of prebiotics, 0-5 parts by weight of choline, 0-10 parts by weight of phospholipids, 0-20 parts by weight of vitamins, and 0-30 parts by weight of minerals.

[0034] According to a specific embodiment of the present invention, the infant formula food (including powdered infant formula food and / or liquid infant formula milk) of the present invention, in terms of dry matter, has a total protein content of 7.8g-22.8g / 100g, a whey protein content of 40%-75% of the total protein, a fat content of 14.9g-33.8g / 100g, and a carbohydrate content of 40.7g-79.3g / 100g; the powdered infant formula has a total protein content of 16.5g-30g / 100g, a fat content of 10g-35g / 100g, and a carbohydrate content of 40g-70g / 100g.

[0035] Preferably, the infant formula has a total protein content of 10g-18g / 100g on a dry matter basis; whey protein accounts for 40%-70% of the total protein; fat content of 18g-30g / 100g; and carbohydrate content of 52g-60g / 100g. The modified infant formula has a total protein content of 17g-25g / 100g, a fat content of 12g-25g / 100g, and a carbohydrate content of 45g-60g / 100g on a dry matter basis.

[0036] According to a specific embodiment of the present invention, the infant formula food (including powdered infant formula food and / or liquid infant formula milk) of the present invention has a linoleic acid content of 2700-6000 mg / 100g and an α-linolenic acid content of 270-600 mg / 100g in terms of dry matter.

[0037] According to a specific embodiment of the present invention, the infant formula foods of the present invention include formula foods for infants aged 0-6 months, formula foods for older infants aged 6-12 months, and formula foods for toddlers aged 12-36 months; wherein: the formula foods for infants aged 0-6 months, in terms of dry matter content, have a total protein content of 8.5-16g / 100g, a whey protein content of 60-75% of the total protein, a fat content of 20.5-32.5g / 100g, a linoleic acid content of 2700-6000mg / 100g, an α-linolenic acid content of 270-600mg / 100g, and a carbohydrate content of 50-60g / 100g; the formula foods for older infants aged 6-12 months, in terms of dry matter content... In the formula, the total protein content is 10-20g / 100g, with whey protein accounting for 40-75% of the total protein; the fat content is 20-30g / 100g; the linoleic acid content is 2700-4500mg / 100g; the α-linolenic acid content is 270-450mg / 100g; and the carbohydrate content is 50-60g / 100g. For infant formula for 12-36 months, the dry matter content is 10-20g / 100g, the fat content is 20-30g / 100g, the linoleic acid content is 2700-4500mg / 100g, the α-linolenic acid content is 270-450mg / 100g; and the carbohydrate content is 50-60g / 100g.

[0038] The present invention also provides a method for preparing the above-mentioned infant formula food, wherein the infant formula food includes powdered infant formula food, liquid infant formula milk or powdered children's modified milk powder;

[0039] The preparation methods for powdered infant formula and powdered children's modified milk include the following steps:

[0040] S101: Mixing and Formulation: Mix the corresponding ingredients in proportion according to the type of infant formula to ensure uniform distribution and formula stability, and obtain a mixture.

[0041] S102: Production and Processing: After filtration and impurity removal, the above-mentioned mixed materials are homogenized at a temperature not lower than 50°C and a pressure not lower than 120 bar to obtain homogenized material. The homogenized material is then concentrated, with a dry matter weight content of 40% - 60% to obtain concentrated material. The concentrated material is then sterilized at a temperature above 80°C for 10 to 60 seconds to obtain sterilized material. The sterilized material is then dried in a spray drying tower with an inlet air temperature of 150°C - 200°C, an exhaust air temperature of 80°C - 120°C, a negative pressure of -10 to -2 mmWG, and a high-pressure pump pressure of 140 - 250 bar. The material is then introduced into a fluidized bed for secondary drying to obtain powdered food.

[0042] The preparation method of infant formula liquid milk includes the following steps: fresh milk purification, cold storage, mixing in proportion, high-pressure homogenization, volume adjustment, ultra-high temperature instantaneous sterilization, and aseptic filling. Specifically, after the fresh milk is purified by membrane filtration, it is stored at 2-8℃ for later use. The ingredients are mixed in proportion at 55-65℃ for 10-20 minutes to ensure uniform distribution of the liquid and stability of the formula. After high-pressure homogenization at 300-350 bar, it is cooled and volume adjusted. After ultra-high temperature instantaneous sterilization at 135-150℃ for 2-8 seconds, it is aseptically filled.

[0043] The present invention also provides the application of the above-mentioned infant formula food in the preparation of products that help improve and / or enhance individual visual function.

[0044] In the above applications, preferably, the enhancement and / or improvement of individual visual function includes:

[0045] It helps improve visual ability.

[0046] According to some specific embodiments of the present invention, in the application of the present invention, the contribution to enhancing and / or improving an individual's visual function includes:

[0047] It can improve visual function decline caused by individual lead exposure.

[0048] According to a specific embodiment of the present invention, the visual function or ability includes, but is not limited to, contrast sensitivity, dark adaptation, glare tolerance, and other capabilities.

[0049] According to some specific embodiments of the present invention, in the application of the present invention, the ability to enhance and / or improve an individual's visual function includes: the ability to enhance visual contrast sensitivity.

[0050] According to some specific embodiments of the present invention, in the application of the present invention, the improvement and / or enhancement of an individual's visual function includes the improvement of visual dark adaptation ability.

[0051] According to some specific embodiments of the present invention, in the application of the present invention, the improvement and / or enhancement of an individual's visual function includes the improvement of visual glare tolerance.

[0052] According to some specific embodiments of the present invention, in the application of the present invention, the contribution to improving and / or enhancing an individual's visual function includes: helping to slow down or prevent the decline or deterioration of visual ability.

[0053] In the above applications, preferably, the individual is a child, such as an infant, toddler, or child. It can also be a person with a high blood lead level.

[0054] This definition applies to all parts of this invention involving "children" to ensure that the technology, products, or methods of this invention meet safety standards and are suitable for use by children. The design and usage parameters of specific products or devices may be appropriately adjusted according to the characteristics of different age groups to ensure their safety and effectiveness.

[0055] According to some specific embodiments of the present invention, the product can be any of the aforementioned food products, or a food product made from the aforementioned infant formula food as raw material, without or with the addition of other substances, through further processing.

[0056] In some specific embodiments of the present invention, a growth animal model of lead exposure was constructed, and a 28-day nutritional intervention was conducted to observe the technical effect of the formulated food of the present invention in improving the visual function decline caused by lead exposure. The results showed that the combined application of a specific ratio of galactose and calcium could exert a better synergistic effect, further improving the visual function decline caused by lead exposure, and exhibiting a certain synergistic enhancement effect.

[0057] In summary, the infant formula containing calcium and galactose provided by this invention helps to enhance and / or improve visual function. Attached Figure Description

[0058] Figure 1 This is a schematic diagram of a visual light and dark box.

[0059] Figure 2 Displays the duration of the exposed box area for different groups.

[0060] Figure 3 Displays the number of times different groups entered the open box area. Detailed Implementation

[0061] Before further describing specific embodiments of the present invention, it should be understood that the scope of protection of the present invention is not limited to the specific embodiments described below; it should also be understood that the terminology used in the embodiments of the present invention is for describing specific embodiments and not for limiting the scope of protection of the present invention.

[0062] When numerical ranges are given in the embodiments, it should be understood that, unless otherwise stated in the present invention, the two endpoints of each numerical range and any value between the two endpoints may be selected.

[0063] Unless otherwise defined, all technical and scientific terms used in this invention have the same meaning as commonly understood by one of ordinary skill in the art.

[0064] As used herein, the term "improved visual function" means an enhancement of visual function, including but not limited to improving visual ability, mitigating, slowing down, or preventing visual decline or deterioration, compared to the same response in the absence of the composition comprising calcium and galactose of this invention. The visual function or ability described includes, but is not limited to, abilities such as contrast sensitivity, dark adaptation, and glare tolerance. In relation to any beneficial effect on visual function or ability, the term "improved" is synonymous with "enhanced," "elevated," or "promoted." In some embodiments of this invention, particularly in cases where an individual's visual function is impaired or threatened, the term "improved visual function" means an effect that reduces, mitigates, or alleviates the degree of visual function decline, or reduces the risk of visual function decline or threat, including but not limited to: alleviating adverse symptoms associated with visual function decline; slowing down or preventing the onset of symptoms before they occur; slowing down or preventing the progression of visual function decline; slowing down or preventing the worsening of visual function decline; slowing down or preventing irreversible damage in the progressive (or chronic) phase of visual function decline; delaying the onset of (progressive) visual function decline; reducing the severity of visual function decline; restoring impaired visual function to normal levels; and preventing the occurrence of visual function decline. The term "visual function decline" or "visual impairment" as used in this article refers to a decrease or decline in the ability of visual function, to the point that it is difficult to maintain or achieve normal function without intervention. Some common signs of visual function decline include a decrease in contrast sensitivity, dark adaptation, glare tolerance, and other abilities.

[0065] As used herein, the term “individual” means any animal or human who may benefit from the product of the present invention that helps improve visual function.

[0066] Unless otherwise specified, the term "child" in this instruction manual refers to a person aged 0 to 12 years. Based on different age groups and developmental characteristics, "child" can be further divided into infants, toddlers, or children. The term "infant" refers to a human being aged 0-3 years from birth. Infancy (0-1 year): refers to children from birth to one year of age. Toddlerhood (1-3 years): refers to children aged 1 to 3 years. The term "child" refers to a human being aged 3-15 years from birth.

[0067] In addition to the specific methods, equipment, and materials used in the embodiments, based on the knowledge of those skilled in the art and the description of this invention, any prior art methods, equipment, and materials similar to or equivalent to those described, equipment, and materials in the embodiments of this invention can be used to implement this invention.

[0068] Unless otherwise stated, the experimental methods, detection methods and preparation methods disclosed in this invention all adopt conventional techniques in this technical field.

[0069] In all embodiments and experiments, the added raw materials, including calcium source, D-galactose, and galactooligosaccharides, were all commercially available food-grade raw materials. Unless otherwise specified, the calcium, D-galactose, and galactooligosaccharides mentioned in this invention are based on the effective content of the functional components calcium and D-galactose in the raw materials.

[0070] Example 1

[0071] This embodiment provides a powdered infant formula milk powder with a total protein content of 20g / 100g, a calcium content of 1800mg / 100g, a galactose content of 12g / 100g, a calcium:galactose ratio of 1:6.6, a fat content of 13.5g / 100g, and a carbohydrate content of 46.5g / 100g.

[0072] This embodiment is achieved by compounding the following raw materials in parts by weight, and the raw material composition includes (for preparation of 1000 parts by weight):

[0073] 3400 parts raw milk, 315 parts skim milk powder, 240 parts lactose, 3 parts compound vitamins, 15 parts calcium carbonate, 20 parts milk mineral salts, 2 parts phospholipids, 1 part compound minerals, and 1 part choline.

[0074] The powdered infant formula milk powder of this embodiment is prepared using a combined dry and wet production process. The preparation method is as follows: pasteurization of raw milk, ingredient mixing, blending, preheating, homogenization, concentration and sterilization, spray drying, and dry mixing to obtain the finished product; the specific steps include:

[0075] Raw milk is inspected, coarsely filtered, cooled and stored, then pasteurized (85℃, 15s) and placed in a mixing tank; the main ingredients and other raw materials are thoroughly mixed in proportion to ensure uniform distribution and formula stability, resulting in a mixture.

[0076] After filtration to remove impurities, the mixture is homogenized at a temperature of 50°C and a pressure of 120 bar to obtain homogenized material.

[0077] Subsequently, the homogeneous material was concentrated, and the dry matter content of the concentrate was 50%, thus obtaining the concentrated material.

[0078] The concentrated material is sterilized at 85℃ for 15 seconds to obtain sterilized material.

[0079] The sterilized material is dried in a spray drying tower (inlet air temperature 175℃, exhaust air temperature 100℃, negative pressure -8mmWG, high pressure pump pressure 200 bar), and then introduced into a fluidized bed for secondary drying to obtain powdered infant formula milk powder.

[0080] Testing showed that all indicators of the finished product met the standards for children's formulated milk powder.

[0081] Example 2

[0082] This embodiment provides a powdered infant formula milk powder with a total protein content of 20g / 100g, a calcium content of 695mg / 100g, a galactose content of 12.5g / 100g, a calcium:galactose ratio of 1:18, a fat content of 12g / 100g, and a carbohydrate content of 37.5g / 100g.

[0083] This embodiment is achieved by compounding the following raw materials in parts by weight, and the raw material composition includes (for preparation of 1000 parts by weight):

[0084] 3100 parts raw milk, 350 parts skim milk powder, 250 parts lactose, 2.5 parts compound vitamins, 2 parts phospholipids, 1 part compound minerals, and 1 part choline.

[0085] The powdered infant formula milk powder of this embodiment is prepared using a combined dry and wet production process. The preparation method is as follows: pasteurization of raw milk, ingredient mixing, blending, preheating, homogenization, concentration and sterilization, spray drying, and dry mixing to obtain the finished product; the specific steps include:

[0086] Raw milk is inspected, coarsely filtered, cooled and stored, then pasteurized (85℃, 15s) and placed in a mixing tank; the main ingredients and other raw materials are thoroughly mixed in proportion to ensure uniform distribution and formula stability, resulting in a mixture.

[0087] After filtration to remove impurities, the mixture is homogenized at a temperature of 50°C and a pressure of 120 bar to obtain homogenized material.

[0088] Subsequently, the homogeneous material was concentrated, and the dry matter content of the concentrate was 50%, thus obtaining the concentrated material.

[0089] The concentrated material is sterilized at 85℃ for 15 seconds to obtain sterilized material.

[0090] The sterilized material is dried in a spray drying tower (inlet air temperature 175℃, exhaust air temperature 100℃, negative pressure -8mmWG, high pressure pump pressure 200 bar), and then introduced into a fluidized bed for secondary drying to obtain powdered infant formula milk powder.

[0091] Testing showed that all indicators of the finished product met the standards for children's formulated milk powder.

[0092] Example 3

[0093] This embodiment provides a powdered infant formula milk powder with a total protein content of 27g / 100g, a calcium content of 750mg / 100g, a galactose content of 0.75g / 100g, a calcium:galactose ratio of 1:1, a fat content of 16.5g / 100g, and a carbohydrate content of 48g / 100g.

[0094] This embodiment is achieved by compounding the following raw materials in parts by weight, and the raw material composition includes (for preparation of 1000 parts by weight):

[0095] 4400 parts raw milk, 420 parts skim milk powder, 7.5 parts galactose, 2.5 parts compound vitamins, 2 parts phospholipids, 1 part compound minerals, and 1 part choline.

[0096] The powdered infant formula milk powder of this embodiment is prepared using a combined dry and wet production process. The preparation method is as follows: pasteurization of raw milk, ingredient mixing, blending, preheating, homogenization, concentration and sterilization, spray drying, and dry mixing to obtain the finished product; the specific steps include:

[0097] Raw milk is inspected, coarsely filtered, cooled and stored, then pasteurized (85℃, 15s) and placed in a mixing tank; the main ingredients and other raw materials are thoroughly mixed in proportion to ensure uniform distribution and formula stability, resulting in a mixture.

[0098] After filtration to remove impurities, the mixture is homogenized at a temperature of 50°C and a pressure of 120 bar to obtain homogenized material.

[0099] Subsequently, the homogeneous material was concentrated, and the dry matter content of the concentrate was 50%, thus obtaining the concentrated material.

[0100] The concentrated material is sterilized at 85℃ for 15 seconds to obtain sterilized material.

[0101] The sterilized material is dried in a spray drying tower (inlet air temperature 175℃, exhaust air temperature 100℃, negative pressure -8mmWG, high pressure pump pressure 200 bar), and then introduced into a fluidized bed for secondary drying to obtain powdered infant formula milk powder.

[0102] Testing showed that all indicators of the finished product met the standards for children's formulated milk powder.

[0103] Example 4

[0104] This embodiment provides a powdered infant formula milk powder with a total protein content of 20g / 100g, a calcium content of 443mg / 100g, a galactose content of 1.329g / 100g, a galacto-oligosaccharide content of 12.4g / 100g, a calcium:galactose:galacto-oligosaccharide ratio of 1:3:28, a fat content of 14.5g / 100g, and a carbohydrate content of 45g / 100g.

[0105] This embodiment is achieved by compounding the following raw materials in parts by weight, and the raw material composition includes (for preparation of 1000 parts by weight):

[0106] 3900 parts raw milk, 400 parts skim milk powder, 27 parts galactose, 300 parts galacto-oligosaccharide syrup, 2.5 parts vitamins, 2 parts phospholipids, 1 part compound minerals, and 1 part choline.

[0107] The powdered infant formula milk powder of this embodiment is prepared using a combined dry and wet production process. The preparation method is as follows: pasteurization of raw milk, ingredient mixing, blending, preheating, homogenization, concentration and sterilization, spray drying, and dry mixing to obtain the finished product; the specific steps include:

[0108] Raw milk is inspected, coarsely filtered, cooled and stored, then pasteurized (85℃, 15s) and placed in a mixing tank; the main ingredients and other raw materials are thoroughly mixed in proportion to ensure uniform distribution and formula stability, resulting in a mixture.

[0109] After filtration to remove impurities, the mixture is homogenized at a temperature of 50°C and a pressure of 120 bar to obtain homogenized material.

[0110] Subsequently, the homogeneous material was concentrated, and the dry matter content of the concentrate was 50%, thus obtaining the concentrated material.

[0111] The concentrated material is sterilized at 85℃ for 15 seconds to obtain sterilized material.

[0112] The sterilized material is dried in a spray drying tower (inlet air temperature 175℃, exhaust air temperature 100℃, negative pressure -8mmWG, high pressure pump pressure 200 bar), and then introduced into a fluidized bed for secondary drying to obtain powdered infant formula milk powder.

[0113] Testing showed that all indicators of the finished product met the standards for children's formulated milk powder.

[0114] Example 5

[0115] This embodiment provides a powdered infant formula milk powder with a total protein content of 21g / 100g, a calcium content of 600mg / 100g, a galactose content of 1.2g / 100g, a galacto-oligosaccharide content of 4.8g / 100g, a calcium:galactose:galacto-oligosaccharide ratio of 1:2:8, a fat content of 14.5g / 100g, and a carbohydrate content of 50.5g / 100g.

[0116] This embodiment is achieved by compounding the following raw materials in parts by weight, and the raw material composition includes (for preparation of 1000 parts by weight):

[0117] 3000 parts raw milk, 540 parts skim milk powder, 80 parts galactooligosaccharide syrup, 24 parts lactose, 3 parts calcium carbonate, 2.5 parts compound vitamins, 2 parts phospholipids, 1 part compound minerals, and 1 part choline.

[0118] The powdered infant formula milk powder of this embodiment is prepared using a combined dry and wet production process. The preparation method is as follows: pasteurization of raw milk, ingredient mixing, blending, preheating, homogenization, concentration and sterilization, spray drying, and dry mixing to obtain the finished product; the specific steps include:

[0119] Raw milk is inspected, coarsely filtered, cooled and stored, then pasteurized (85℃, 15s) and placed in a mixing tank; the main ingredients and other raw materials are thoroughly mixed in proportion to ensure uniform distribution and formula stability, resulting in a mixture.

[0120] After filtration to remove impurities, the mixture is homogenized at a temperature of 50°C and a pressure of 120 bar to obtain homogenized material.

[0121] Subsequently, the homogeneous material was concentrated, and the dry matter content of the concentrate was 50%, thus obtaining the concentrated material.

[0122] The concentrated material is sterilized at 85℃ for 15 seconds to obtain sterilized material.

[0123] The sterilized material is dried in a spray drying tower (inlet air temperature 175℃, exhaust air temperature 100℃, negative pressure -8mmWG, high pressure pump pressure 200 bar), and then introduced into a fluidized bed for secondary drying to obtain powdered infant formula milk powder.

[0124] Testing showed that all indicators of the finished product met the standards for children's formulated milk powder.

[0125] Example 6: Experiment on the efficacy of formulated food in improving individual visual function

[0126] In this experiment, a growth animal model of lead exposure was constructed, and a 28-day nutritional intervention was conducted to observe the technical effect of the combination of calcium, galactose, and galactooligosaccharides in the food formula of this invention on improving visual function.

[0127] 1. Animal husbandry

[0128] Seventy-two three-week-old female SD rats were purchased from Beijing Speford Biotechnology Co., Ltd. and housed in the animal facility of China Agricultural University. During the experiment, the animal facility was kept quiet, clean, well-ventilated, and under suitable lighting conditions, with a light-dark cycle of 12 hours, a temperature of 22 ± 1°C, and a relative humidity of 50–60%. Rats were randomly divided into six groups of 12 rats each: a normal group (AIN93G diet), a model group (orally administered 200 mg / kg / day lead acetate, with AIN93G diet containing 2500 mg / kg calcium), a control group 1 (lead exposure intervention group with AIN93G diet containing 5000 mg / kg calcium), a control group 2 (orally administered 200 mg / kg / day lead acetate, with AIN93G diet containing galactose replacing half of the sucrose, achieving a 5% energy ratio), an experimental group 1 (lead exposure intervention group with AIN93G diet containing galactose partially replacing sucrose, achieving a 0.2% energy ratio, and galactooligosaccharides accounting for 40% of dietary fiber), and an experimental group 2 (lead exposure intervention group with AIN93G diet containing galactose partially replacing sucrose, achieving a 1% energy ratio). The diets for each group are shown in Table 1. Sensory indicators of the diets are shown in Table 2.

[0129] Table 1. Feed and intervention details for each group

[0130]

[0131] Note: In Experiment 1, 20g of the 50g dietary fiber in the feed was galactooligosaccharide, while the dietary fiber in other groups was the standard dietary fiber in AIN93G feed.

[0132] In Experiment Example 1, the dietary design of the feed formula was: galactose energy accounted for 0.2% of the total dietary energy; compared with the model group, the dietary calcium supplementation was 50% of the recommended intake (RNI). Under these conditions, based on the equivalence of the population diet, the mass ratio of galactose to calcium can be estimated as follows: (1) According to the dietary habits of a 3-year-old girl (energy requirement 1150 kcal / d, recommended calcium intake: 600 mg / d), the corresponding amount of galactose is about 0.568 g / d, and the ratio with the calcium intake of 600 mg / d is about 1:1. Considering that the calcium intake of other daily diets is about 1 / 2 of the RNI, the ratio of galactose to calcium in the formula food of this invention as a nutritional supplement can be about 2:1. (2) Based on the dietary habits of a 15-year-old boy (energy requirement 2950 kcal / d, recommended calcium intake 1000 mg / d), the corresponding amount of galactose is about 1.458 g / d, and the ratio of galactose to calcium intake of 1000 mg / d is about 1.5:1. Considering that the calcium intake of other daily diets is about 1 / 2 of the RNI, the ratio of galactose to calcium in the formula food of the present invention as a nutritional supplement can be about 3:1.

[0133] In Experiment 1, the feed formulation was designed to contain 40% galactooligosaccharides as dietary fiber, corresponding to a dietary energy ratio of approximately 0.9%. Under these conditions, based on the equivalence of the population diet, the mass ratio of galactooligosaccharides to calcium can be estimated as follows: (1) Based on the dietary habits of a 3-year-old girl, the ratio of galactooligosaccharides (5.43 g / d) to calcium intake is approximately 9:1. Considering the calcium intake from other daily diets, the ratio of galactooligosaccharides to calcium in the formula food of this invention as a nutritional supplement can be designed to be approximately 18:1; (2) Based on the dietary habits of a 15-year-old boy, the ratio of galactooligosaccharides (13.94 g / d) to calcium intake is approximately 14:1. Considering the calcium intake from other daily diets, the ratio of galactooligosaccharides to calcium in the formula food of this invention as a nutritional supplement can be designed to be approximately 28:1.

[0134] In Experiment Example 2, the dietary design of the feed formula was as follows: the energy ratio of galactose accounted for 1% of the total dietary energy; compared with the model group, the dietary calcium supplementation was 50% of the recommended intake (RNI). Under these conditions, based on the equivalence of the population diet, (1) according to the dietary habits of 3-year-old girls, the ratio of galactose to calcium intake was estimated to be about 4.5:1. Considering the calcium intake of other daily diets, the ratio of galactose to calcium in the formula food of this invention as a nutritional supplement can be designed to be about 9:1; (2) according to the dietary habits of 15-year-old boys, the ratio of galactose to calcium intake was estimated to be about 7.3:1. Considering the calcium intake of other daily diets, the ratio of galactose to calcium in the formula food of this invention as a nutritional supplement can be designed to be about 15:1.

[0135] Table 2. Sensory indicators of sucrose, galactose, dietary fiber, and mineral packets in each group of feeds after being formulated separately.

[0136]

[0137] 2. Visual-Light Box Behavioral Evaluation

[0138] Taking advantage of rodents' aversion to light, their visual function was analyzed by measuring the time, distance traveled, and number of times they entered a brightly lit box. Experimental equipment included... Figure 1 As shown, the experimental animal was placed in the center of a bright box, and a camera recorded its duration and trajectory within the box for 5 minutes. These metrics were then analyzed. Experimental equipment included... Figure 1 As shown.

[0139] 3. Statistical methods

[0140] All data are expressed as mean ± SEM. Statistical analysis was performed using GraphPad Prism 8 software. One-way ANOVA was used to compare multiple groups, and a p-value < 0.05 was considered statistically significant.

[0141] 4. Experimental Results

[0142] The specific results are shown in Table 3. Figure 2 , Figure 3 Rodents are known to be afraid of light sources. The model group (lead exposure + low calcium model) had the highest duration of activity in the bright box area and the highest number of times it entered the bright box area. This means that lead exposure and low calcium intake caused rats to move more frequently in the bright box area, indicating that lead exposure and low dietary calcium intake caused a decline in visual function.

[0143] Table 3

[0144]

[0145] Note: Different letters in the table indicate significant differences.

[0146] Compared with the model, Comparative Example 1 showed a decreasing trend in the time spent in the open box and the number of times the model entered the open box under calcium intervention, but this was not statistically significant, indicating that relying solely on dietary calcium supplementation cannot achieve the expected protective effect on visual function.

[0147] Compared to Comparative Example 1, Comparative Example 2 showed a decreasing trend in time spent in the open box and the number of times the participant entered the open box under galactose intervention, but this was not statistically significant. This indicates that relying solely on high-dose galactose supplementation cannot achieve the expected protective effect on visual function. Compared to the model group, Comparative Example 2 showed a decreasing trend in time spent in the open box and the number of times the participant entered the open box within a galactose:calcium ratio of (47-73):1, but this was not statistically significant. This suggests that the ratio of galactose to calcium requires careful design and exploration to achieve unexpected results.

[0148] Compared with the model group, Comparative Example 1 and Comparative Example 2, Experimental Example 1 showed a significant decrease in the time spent in the open box area and the number of times it entered the open box area within the ratio range of galactose:calcium = (2-3):1 and within the ratio range of galactose:galactooligosaccharide:calcium = (2-3):(18-28):1. The number of times it entered the open box area reached the level of the normal control group, achieving an unexpected visual function protection effect.

[0149] Compared with the model group, in Experiment 2, within the ratio range of galactose:calcium = (9-15):1, the time spent in the open box area and the number of times entering the open box area both showed a significant decrease. The time spent in the open box area was significantly different from that of Comparative Example 1 and showed a clear downward trend compared with Comparative Example 2. The number of times entering the open box area also showed a clear downward trend compared with Comparative Examples 1 and 2, achieving an unexpected visual function protection effect.

[0150] The above description of the embodiments is provided to enable those skilled in the art to understand and use the invention. It will be apparent to those skilled in the art that various modifications can be made to these embodiments, and the general principles described herein can be applied to other embodiments without inventive effort. Therefore, the present invention is not limited to the above embodiments, and any improvements and modifications made by those skilled in the art based on the disclosure of the present invention without departing from the scope of the invention should be within the protection scope of the present invention.

Claims

1. A pediatric formula comprising calcium and galactose, wherein, The mass ratio of calcium to galactose is 1:(1-18); The total protein content is 7.8-30 g / 100 g, the fat content is 10-35 g / 100 g, and the carbohydrate content is 37.5-79.3 g / 100 g based on the dry matter of the infant formula.

2. The pediatric formula of claim 1, wherein, The mass ratio of calcium to galactose is 1:(1-6) or 1:(9-15).

3. The infant formula according to claim 1 or 2, further comprising galacto-oligosaccharides, and the mass ratio of the galacto-oligosaccharides to calcium is (9-28):

1.

4. The infant formula according to claim 1, wherein the amount of calcium is 130-1800 mg / 100 g of dry matter of the infant formula, the amount of galactose is 0.2-32.5 g / 100 g of dry matter of the infant formula, and the amount of galacto-oligosaccharides is 0-25 g / 100 g of dry matter of the infant formula.

5. The pediatric formula of any one of claims 1-4, wherein, The infant formula is a powdered infant formula, an infant formula liquid milk, or a powdered children's formula milk powder, wherein the powdered infant formula includes a powdered infant formula, a powdered older infant formula, and a powdered young child formula, and the infant formula liquid milk includes an infant formula liquid milk, an older infant formula liquid milk, and a young child formula liquid milk.

6. The pediatric formula of claim 5, wherein, The raw material composition of the powdered infant formula includes 0-7000 parts by weight of raw cow milk, 0-600 parts by weight of desalted whey powder, 0-500 parts by weight of lactose, 0-600 parts by weight of skim milk powder, 0-400 parts by weight of edible vegetable blend oil, 0-100 parts by weight of prebiotics, 0-120 parts by weight of whey protein powder, 0-20 parts by weight of phospholipids, 0-20 parts by weight of vitamins, 0-40 parts by weight of casein, and 0-30 parts by weight of minerals, in preparation of 1000 parts by weight of the powdered infant formula. The raw material composition of the infant formula liquid milk includes 0-7000 parts by weight of raw cow milk, 0-500 parts by weight of skim milk powder, 0-500 parts by weight of desalted whey powder, 0-500 parts by weight of lactose, 0-400 parts by weight of edible vegetable blend oil, 0-100 parts by weight of prebiotics, 0-120 parts by weight of whey protein powder, 0-10 parts by weight of phospholipids, 0-40 parts by weight of casein, 0-20 parts by weight of vitamins, and 0-30 parts by weight of minerals, in preparation of 1000 parts by weight of the infant formula liquid milk. The raw material composition of the powdered children's formula milk powder includes 0-7000 parts by weight of raw cow milk, 0-500 parts by weight of skim milk powder, 0-600 parts by weight of desalted whey powder, 0-120 parts by weight of whey protein powder, 0-500 parts by weight of lactose, 0-50 parts by weight of prebiotics, 0-5 parts by weight of choline, 0-10 parts by weight of phospholipids, 0-20 parts by weight of vitamins, and 0-30 parts by weight of minerals, in preparation of 1000 parts by weight of the powdered children's formula milk powder.

7. The pediatric formula of claim 5 or 6, wherein, The pediatric formula includes linoleic acid and alpha-linolenic acid, the content of linoleic acid is 2700-6000 mg / 100 g, and the content of alpha-linolenic acid is 270-600 mg / 100 g based on the dry matter of the pediatric formula.

8. A process for the preparation of a pediatric formula as claimed in any one of claims 1 to 7 wherein, The pediatric formula includes powdered infant formula, infant formula liquid milk or powdered children's formula milk powder; The preparation method of the powdered infant formula and the powdered children's formula milk includes the following steps: S101: mixing and preparation: the corresponding ingredients are mixed in proportion according to the type of the pediatric formula, and the uniform distribution and stability of the formula are ensured to obtain the mixed material; S102: production and processing: the above mixed material is subjected to homogenization treatment after filtering impurities, the homogenization temperature is not less than 50℃, and the pressure is not less than 120 bar to obtain the homogenized material, the homogenized material is concentrated, the dry matter content of the concentrate is 40%-60%, the concentrated material is obtained, the concentrated material is subjected to sterilization treatment at 80℃ or above for 10-60 seconds to obtain the sterilized material, and the sterilized material is dried through a spray drying tower, the inlet air temperature is 150℃-200℃, the exhaust air temperature is 80℃-120℃, the negative pressure is-10 to-2mmWG, and the high-pressure pump pressure is 140-250 bar, then the material is introduced into a fluidized bed for secondary drying to obtain the powdered food; The preparation method of the infant formula liquid milk includes the following steps: fresh milk clarification, cold storage, proportioning, high-pressure homogenization, constant volume, ultra-high temperature instant sterilization, and sterile filling, specifically, the fresh milk is clarified after membrane filtration and stored at 2-8℃ for use, the ingredients are mixed after being proportioned and melted at 55-65℃ for 10-20 min to ensure uniform distribution of the material and stability of the formula, and the material is cooled and filled after high-pressure homogenization at 300-350 bar, and the material is sterilely filled after ultra-high temperature instant sterilization at 135-150℃ for 2-8 s.

9. Use of the pediatric formula of any one of claims 1-7 in the preparation of a product that helps to enhance and / or improve visual function of an individual.

10. Use according to claim 9, wherein, The help to enhance and / or improve visual function of an individual includes: helping to enhance visual ability; helping to enhance visual contrast sensitivity; helping to improve visual dark adaptation ability; helping to improve visual glare tolerance ability; and / or helping to slow down or stop the decline or recession of visual ability.

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