Micro-emulsification inclusion method of asiaticoside in scar-removing gel matrix

Abstract

The invention discloses a scar-removing gel matrix asiaticoside micro-emulsification inclusion method, and relates to the technical field of cosmetic preparation, the scar-removing gel matrix asiaticoside micro-emulsification inclusion method comprises the following steps: S1, adding hydroxypropyl-beta-cyclodextrin into a reaction kettle, adding deionized water, stirring, adding an asiaticoside modified liquid, and stirring to obtain an inclusion compound solution; s2, corn oil, Tween 80, absolute ethyl alcohol and propylene glycol are mixed and stirred, the inclusion compound solution is added and stirred, deionized water is added and stirred, polysorbate 20 and tert-butylhydroquinone are added and stirred, and asiaticoside microemulsion is obtained; s3, adding the asiaticoside microemulsion into the gel matrix and stirring, adding the panthenol modified liquid and stirring, adding triethanolamine and stirring, and adding ethyl p-hydroxybenzoate and stirring to obtain scar-removing gel matrix asiaticoside; according to the invention, the panthenol modified liquid is added, so that the transdermal efficiency of the panthenol is improved, anchoring retention of the panthenol in the skin cuticle is realized, the aging of barrier repair is prolonged, and the storage stability of the gel can also be improved.

Description

Technical Field

[0001] This invention relates to the field of cosmetic preparation technology, and in particular to a method for microemulsifying and encapsulating asiaticoside in a scar-reducing gel matrix. Background Technology

[0002] Inclusion methods are techniques that embed guest molecules into the hollow structure of host molecules through physical interactions, primarily relying on intermolecular forces and requiring no covalent bonds. Commonly used methods include coprecipitation, grinding, saturated aqueous solutions, freeze-drying, and spray drying.

[0003] Traditional methods for encapsulating asiaticoside in scar-reducing gel matrices using microemulsions suffer from several drawbacks. asiaticoside itself is crystalline with low solubility, is easily oxidized and degraded, lacks targeting specific scar tissue, has low transdermal efficiency, and a short residence time on the skin. Panthenol is easily lost with skin moisture, resulting in insufficient time for skin barrier repair and poor transdermal effects. Microemulsion systems are prone to demulsification and particle aggregation, exhibiting poor particle size stability and affecting the encapsulation of active ingredients. The gel matrix is ​​sticky, has poor spreadability, insufficient moisturizing ability, and can lead to rapid release of active ingredients, preventing long-term efficacy at the scar site. Furthermore, the gel is susceptible to pH fluctuations and changes in physicochemical properties during storage, leading to further degradation and loss of active ingredients. The existing system also suffers from high skin irritation, poor antiseptic and antibacterial effects, and susceptibility to microbial growth. Therefore, this invention provides a method for encapsulating asiaticoside in a scar-reducing gel matrice using microemulsions. Summary of the Invention

[0004] The main objective of this invention is to provide a method for microemulsifying and encapsulating asiaticoside in a scar-removing gel matrix with a long shelf life and high β-glucan content, which is applied in a method for microemulsifying and encapsulating asiaticoside in a scar-removing gel matrix.

[0005] To achieve the above objectives, the technical solution adopted by the present invention is as follows: This invention provides a method for microemulsifying and encapsulating asiaticoside in a scar-removing gel matrix, the method comprising the following steps: S1. Hydroxypropyl-β-cyclodextrin was added to a reaction vessel, deionized water was added and stirred, the water bath temperature was set to 45℃, the stirring speed was 100 rpm, and the stirring was kept constant for 20 minutes. Centella asiatica glycoside modified solution was added and stirred, the temperature was set to 45℃, the stirring speed was 100 rpm, and the stirring was kept constant for 4 hours to obtain a mixture. The mixture was refrigerated, the temperature was set to 4℃, and the refrigeration was carried out for 15 minutes. The particles were filtered out using a 0.45μm microporous membrane to obtain an inclusion complex solution. S2. Mix corn oil, Tween 80, anhydrous ethanol and propylene glycol, set the water bath temperature to 37°C, the stirring speed to 500 rpm, and stir at a constant temperature for 15 minutes. Add the inclusion complex solution and stir, set the temperature to 37°C, the stirring speed to 500 rpm, and stir for 10 minutes. Add deionized water and stir, set the stirring speed to 800 rpm, and stir for 20 minutes. Add polysorbate 20 and tert-butylhydroquinone and stir, set the stirring speed to 600 rpm, and stir for 5 minutes to obtain asiaticoside microemulsion. S3. Add the asiaticoside microemulsion to the gel matrix and stir at 500 rpm for 15 minutes. Add the panthenol modification solution and stir at 500 rpm for 8 minutes. Add triethanolamine and stir at 300 rpm for 5 minutes. Add ethylparaben and stir at 300 rpm for 5 minutes. Filter the gel particles using a 0.8 μm microporous membrane to obtain the scar-removing gel matrix asiaticoside. The scar-removing gel matrix, containing asiaticoside, comprises the following raw materials: 20-24 parts hydroxypropyl-β-cyclodextrin, 1.7-1.9 parts asiaticoside modifying solution, 14-18 parts corn oil, 43-47 parts Tween 80, 8-10 parts anhydrous ethanol, 8-10 parts propylene glycol, 0.07-0.09 parts polysorbate 20, 0.02-0.04 parts tert-butylhydroquinone, 40-42.4 parts gel matrix, 2.5-2.7 parts panthenol modifying solution, and 0.09-0.11 parts ethylparaben.

[0006] The mass ratio of hydroxypropyl-β-cyclodextrin to deionized water in S1 is 11:25.

[0007] The mass ratio of deionized water to Tween 80 in S2 is 0.38:1.

[0008] Hydroxypropyl-β-cyclodextrin has a purity of ≥98%, is pharmaceutical grade, has a degree of substitution of 5-7, and a cavity size of 0.7-0.8 nm. It has excellent water solubility and can form a fourfold stable inclusion complex with modified asiaticoside, significantly improving the solubilization effect and encapsulation rate of asiaticoside.

[0009] The corn oil has a purity of ≥99%, is pharmaceutical / food grade, has a viscosity of 30-40 mPa·s at 25℃, and an acid value of ≤0.5 mgKOH / g. Its moderate viscosity and good compatibility with the HLB values ​​of emulsifiers and co-emulsifiers can effectively expand the stable region of microemulsions and provide a uniform dispersion carrier for asiaticoside inclusion complexes.

[0010] Tween 80 has a purity of ≥99%, is pharmaceutical grade, has an HLB value of 15.0, is readily soluble in both water and oil phases, effectively reduces the surface tension between the oil and water phases, promotes the formation of microemulsion systems, stabilizes the interfacial film of microemulsions, and prevents microemulsion particle aggregation. It has strong drug loading capacity, can efficiently carry asiaticoside inclusion complexes, and has excellent compatibility with all components, ensuring that the microemulsion system remains clear and transparent throughout the process.

[0011] Anhydrous ethanol has a purity of ≥95% and is pharmaceutical grade.

[0012] Polysorbate 20 has a purity of ≥99%, is pharmaceutical grade, and has an HLB value of 16.7. It can precisely stabilize the nanoparticles of microemulsions, prevent the aggregation and increase in particle size of microemulsion particles during long-term storage of the gel, and effectively improve the storage stability of the gel.

[0013] Tert-butylhydroquinone has a purity of ≥99%, is pharmaceutical / food grade, and has a melting point of 126-128℃. It has strong antioxidant properties, and adding a small amount can effectively protect active ingredients such as asiaticoside and panthenol from oxidative degradation, improve the storage stability of the gel, and ensure the content of active ingredients within the shelf life.

[0014] Ethyl paraben has a purity of ≥99%, is pharmaceutical grade, and has a melting point of 116-118℃. It has a broad antibacterial spectrum and stable antibacterial effect. Adding a small amount can effectively inhibit the growth of bacteria, mold and other microorganisms in the gel and extend the shelf life of the gel.

[0015] Triethanolamine purity ≥99%, pharmaceutical grade, boiling point: 360℃.

[0016] Furthermore, the gel matrix is ​​prepared by mixing carbomer 940, carbomer 980 and deionized water.

[0017] Furthermore, the mass ratio of Carbomer 940, Carbomer 980 and deionized water is 2.1:0.9:100.

[0018] Carbomer 940 has a purity of ≥99%, is pharmaceutical grade, has a solid content of ≥98%, and a viscosity of 40,000-60,000 mPa·s at 25°C for a 1% aqueous solution.

[0019] Carbomer 980 has a purity of ≥99%, is pharmaceutical grade, has a solid content of ≥98%, and a viscosity of 1% aqueous solution at 25℃ of 100,000-120,000 mPa·s.

[0020] Furthermore, the preparation of the asiaticoside modified solution includes the following steps: A1. Place asiaticoside, anhydrous lactose and maltol into an agate mortar and grind them at a speed of 300-400 rpm for 5 minutes to obtain a mixed powder; A2. Add deionized water and chitosan quaternary ammonium salt to the mixed powder and stir. Set the speed to 300 rpm and stir magnetically for 5 minutes to obtain the modified Centella asiatica extract.

[0021] The purity of asiaticoside is ≥98% by HPLC, and its molecular weight is 480.49D. It can directly inhibit the abnormal deposition of collagen in scar tissue and exert its scar-removing effect from the root, which is the basis for the core function of the gel.

[0022] Anhydrous lactose has a purity of ≥99.5%, is pharmaceutical injection grade, and has a molecular weight of 342.30 Da. It is highly safe, non-irritating and residue-free when used externally, and has excellent compatibility with asiaticoside. During the grinding process, it can efficiently destroy the crystal lattice of asiaticoside through mechanical force, transforming it into an amorphous structure, significantly improving the solubility and dissolution rate of asiaticoside, laying a good foundation for subsequent inclusion and microemulsion preparation.

[0023] Maltol has a purity of ≥99%, is pharmaceutical / food grade, and has a molecular weight of 126.11 Da. It can form stable hydrogen bonds with amorphous asiaticoside through hydroxyl and carbonyl groups to prevent recrystallization, and can also protect the glycosidic bonds of asiaticoside from oxidative degradation by the strong antioxidant properties of phenolic hydroxyl groups, thereby improving the chemical stability of the active ingredient. At the same time, it has a mild anti-inflammatory effect and can form a synergistic scar-reducing effect with asiaticoside.

[0024] Furthermore, the mass ratio of asiaticoside, anhydrous lactose, and maltol is 3:6:1.

[0025] Furthermore, the mass ratio of the mixed powder, deionized water, and chitosan quaternary ammonium salt is 1.5:2:0.3.

[0026] Furthermore, the chitosan quaternary ammonium salt is one of hydroxypropyltrimethylammonium chloride chitosan, carboxymethyltrimethylammonium chloride chitosan, and methyltrimethylammonium chloride chitosan.

[0027] Chitosan quaternary ammonium salt can form a stable electrostatic complex with asiaticoside-maltol complex, giving asiaticoside precise cationic targeting to scar fibroblasts and significantly increasing skin retention time; at the same time, its cationic structure can form an electrostatic interaction with the anionic components in the gel, helping to stabilize the microemulsion interface film, and has low skin irritation, can directly contact damaged scar tissue, and is suitable for use on sensitive skin and damaged scar sites.

[0028] Hydroxypropyltrimethylammonium chloride chitosan has a molecular weight of 30,000 to 50,000 Da (low molecular weight range), a degree of quaternization of 90% to 95%, and a water solubility of ≥99%. It is cosmetic / pharmaceutical grade.

[0029] Carboxymethyl trimethylammonium chloride chitosan has a molecular weight of 30,000 to 50,000 Da, a degree of quaternization of 90% to 92%, and a water solubility of ≥98% at room temperature. It is cosmetic / pharmaceutical grade.

[0030] Methyltrimethylammonium chloride chitosan has a molecular weight of 20,000 to 40,000 Da, a degree of quaternization of 92% to 95%, and a water solubility of ≥97% at room temperature. It is pharmaceutical grade.

[0031] Furthermore, the preparation of the panthenol modified solution includes the following steps: panthenol, hydroxypropyl-α-cyclodextrin and γ-polyglutamic acid are added to a reaction vessel, deionized water is added and stirred, the stirring speed is set to 300 rpm, and magnetic stirring is performed for 8 minutes to obtain the panthenol modified solution.

[0032] Panthenol has a purity of ≥99%, is pharmaceutical grade, and has a molecular weight of 205.24 Da. It can effectively repair the damaged skin barrier in scar areas, relieve skin dryness and discomfort, reduce external stimulation to scar areas, and form a synergistic effect with Centella asiatica glycoside in scar removal and repair.

[0033] Hydroxypropyl-α-cyclodextrin has a purity of ≥98%, is pharmaceutical grade, has a degree of substitution of 4-6, and a cavity size of 0.5-0.6 nm. It achieves precise inclusion of panthenol, effectively reduces the water solubility of panthenol, enhances its ability to penetrate the stratum corneum, and prevents the volatilization and loss of panthenol.

[0034] The γ-polyglutamic acid has a purity of ≥90% (cosmetic grade) and a molecular weight of ≤100,000 Da. It can form stable hydrogen bonds and electrostatic complexes with panthenol-hydroxypropyl-α-cyclodextrin inclusion complex, anchoring panthenol to the stratum corneum of the skin and significantly prolonging the time of barrier repair. At the same time, as an anionic peptide, it can form intermolecular forces with carbomer and Tween 80 in the gel, helping to stabilize the microemulsion interface film, improve the storage stability of the gel, and has its own moisturizing effect. It is highly safe, non-irritating to the skin, and can further reduce the overall irritation of the gel.

[0035] Furthermore, the mass ratio of panthenol, hydroxypropyl-α-cyclodextrin, γ-polyglutamic acid, and deionized water is 4:8:1:10.

[0036] Furthermore, the addition of triethanolamine adjusts the pH conditions to 6-6.5.

[0037] The present invention has the following beneficial effects: 1. In this invention, a modified solution of asiaticoside is added. As the core scar-reducing ingredient, asiaticoside can directly inhibit abnormal collagen deposition in scar tissue, improving scar formation from the root. Anhydrous lactose, maltol, and low molecular weight chitosan quaternary ammonium salt transform crystalline asiaticoside into an amorphous structure through triple modification effects of crystal form regulation, hydrogen bonding, and cationic targeting. This solves the problems of poor water solubility and easy oxidation and degradation of the original asiaticoside, and also gives it precise targeting to scar fibroblasts. At the same time, the modified overall structure can efficiently form a quadruple stable inclusion complex with hydroxypropyl-β-cyclodextrin, perfectly matching the subsequent microemulsification inclusion process, greatly improving the transdermal efficiency and skin retention time of asiaticoside, allowing the scar-reducing active ingredient to act precisely and for a long time on scar tissue, ensuring that the microemulsified asiaticoside fully exerts its scar-reducing effect.

[0038] 2. In this invention, panthenol modification solution is added, which can form a synergistic effect with asiaticoside and the modified materials as a whole, while also helping to optimize the overall performance of the microemulsion inclusion gel. Panthenol, as the core skin repair ingredient, can effectively repair damaged skin barriers, alleviate skin discomfort at scar sites, and reduce external irritation to scar sites. Hydroxypropyl-α-cyclodextrin and low molecular weight γ-polyglutamic acid, through precise inclusion and supramolecular complexation, enhance the transdermal efficiency of panthenol and achieve its anchoring and retention in the stratum corneum, prolonging the barrier repair time. Furthermore, this modification is completely compatible with the microemulsion inclusion system and gel matrix, without damaging the microemulsion particle structure or gel formation. Simultaneously, the γ-polyglutamic acid it contains can form intermolecular forces with the gel components, helping to stabilize the microemulsion interface film and improve the storage stability of the gel. While achieving repair efficacy, it further optimizes the safety and storage stability of the asiaticoside microemulsion inclusion gel.

[0039] 3. In this invention, a gel matrix is ​​added, which is prepared by compounding Carbomer 940 and Carbomer 980. It retains the good gelling properties and three-dimensional gel framework support of carbomer materials, and can efficiently and uniformly support the asiaticoside microemulsion system and the dual-modified material as a whole. The gel framework enables the slow release of active ingredients and prolongs the duration of drug efficacy. Through precise optimization of the compounding ratio, the problems of stickiness caused by single carbomer gel and poor spreadability caused by cyclodextrin dosage are solved, giving the gel excellent application characteristics such as easy application, no residue, and good skin adhesion. At the same time, the anionic structure of the matrix can form electrostatic and intermolecular forces with the cationic asiaticoside modified whole and the anionic panthenol modified whole, realizing the anchoring of the dual-modified material as a whole, further improving the skin retention of active ingredients. It also has excellent compatibility with the microemulsion system, which can ensure that the microemulsion particles are uniformly dispersed in the gel and avoid problems such as gel layering and crystallization. This provides key guarantees for the appearance, user experience, drug efficacy release, and storage stability of the asiaticoside microemulsion-encapsulated scar removal gel. Detailed Implementation

[0040] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0041] It should be noted that all raw materials used in the following experiments are commercially available.

[0042] Example 1: A method for microemulsifying and encapsulating asiaticoside in a scar-reducing gel matrix. The method includes the following steps: S1. Hydroxypropyl-β-cyclodextrin was added to a reaction vessel, deionized water was added and stirred, the water bath temperature was set to 45℃, the stirring speed was 100 rpm, and the stirring was kept constant for 20 minutes. Centella asiatica glycoside modified solution was added and stirred, the temperature was set to 45℃, the stirring speed was 100 rpm, and the stirring was kept constant for 4 hours to obtain a mixture. The mixture was refrigerated, the temperature was set to 4℃, and the refrigeration was carried out for 15 minutes. The particles were filtered out using a 0.45μm microporous membrane to obtain an inclusion complex solution. S2. Mix corn oil, Tween 80, anhydrous ethanol and propylene glycol, set the water bath temperature to 37°C, the stirring speed to 500 rpm, and stir at a constant temperature for 15 minutes. Add the inclusion complex solution and stir, set the temperature to 37°C, the stirring speed to 500 rpm, and stir for 10 minutes. Add deionized water and stir, set the stirring speed to 800 rpm, and stir for 20 minutes. Add polysorbate 20 and tert-butylhydroquinone and stir, set the stirring speed to 600 rpm, and stir for 5 minutes to obtain asiaticoside microemulsion. S3. Add the asiaticoside microemulsion to the gel matrix and stir at 500 rpm for 15 minutes. Add the panthenol modification solution and stir at 500 rpm for 8 minutes. Add triethanolamine and stir at 300 rpm for 5 minutes. Add ethylparaben and stir at 300 rpm for 5 minutes. Filter the gel particles using a 0.8 μm microporous membrane to obtain the scar-removing gel matrix asiaticoside. The scar-removing gel matrix, containing asiaticoside, comprises the following raw materials: 20 parts hydroxypropyl-β-cyclodextrin, 1.7 parts asiaticoside modification solution, 14 parts corn oil, 43 parts Tween 80, 8 parts anhydrous ethanol, 8 parts propylene glycol, 0.07 parts polysorbate 20, 0.02 parts tert-butylhydroquinone, 40 parts gel matrix, 2.5 parts panthenol modification solution, and 0.09 parts ethylparaben.

[0043] The gel matrix was prepared by mixing carbomer 940, carbomer 980 and deionized water.

[0044] The mass ratio of Carbomer 940, Carbomer 980 and deionized water is 2.1:0.9:100.

[0045] The preparation of the asiaticoside modified solution includes the following steps: A1. Place asiaticoside, anhydrous lactose and maltol into an agate mortar and grind for 5 minutes at a speed of 300 rpm to obtain a mixed powder; A2. Add deionized water and chitosan quaternary ammonium salt to the mixed powder and stir. Set the speed to 300 rpm and stir magnetically for 5 minutes to obtain the modified Centella asiatica extract.

[0046] The mass ratio of asiaticoside, anhydrous lactose, and maltol is 3:6:1.

[0047] The mass ratio of the mixed powder, deionized water, and chitosan quaternary ammonium salt is 1.5:2:0.3.

[0048] The chitosan quaternary ammonium salt is one of hydroxypropyltrimethylammonium chloride chitosan, carboxymethyltrimethylammonium chloride chitosan, and methyltrimethylammonium chloride chitosan.

[0049] The preparation of panthenol modified solution includes the following steps: panthenol, hydroxypropyl-α-cyclodextrin and γ-polyglutamic acid are added to a reaction vessel, deionized water is added and stirred, the stirring speed is set to 300 rpm and magnetic stirring is performed for 8 minutes to obtain panthenol modified solution.

[0050] The mass ratio of panthenol, hydroxypropyl-α-cyclodextrin, γ-polyglutamic acid, and deionized water was 4:8:1:10.

[0051] Add triethanolamine to adjust the pH to 6.

[0052] Example 2: A method for microemulsifying and encapsulating asiaticoside in a scar-reducing gel matrix. The method includes the following steps: S1. Hydroxypropyl-β-cyclodextrin was added to a reaction vessel, deionized water was added and stirred, the water bath temperature was set to 45℃, the stirring speed was 100 rpm, and the stirring was kept constant for 20 minutes. Centella asiatica glycoside modified solution was added and stirred, the temperature was set to 45℃, the stirring speed was 100 rpm, and the stirring was kept constant for 4 hours to obtain a mixture. The mixture was refrigerated, the temperature was set to 4℃, and the refrigeration was carried out for 15 minutes. The particles were filtered out using a 0.45μm microporous membrane to obtain an inclusion complex solution. S2. Mix corn oil, Tween 80, anhydrous ethanol and propylene glycol, set the water bath temperature to 37°C, the stirring speed to 500 rpm, and stir at a constant temperature for 15 minutes. Add the inclusion complex solution and stir, set the temperature to 37°C, the stirring speed to 500 rpm, and stir for 10 minutes. Add deionized water and stir, set the stirring speed to 800 rpm, and stir for 20 minutes. Add polysorbate 20 and tert-butylhydroquinone and stir, set the stirring speed to 600 rpm, and stir for 5 minutes to obtain asiaticoside microemulsion. S3. Add the asiaticoside microemulsion to the gel matrix and stir at 500 rpm for 15 minutes. Add the panthenol modification solution and stir at 500 rpm for 8 minutes. Add triethanolamine and stir at 300 rpm for 5 minutes. Add ethylparaben and stir at 300 rpm for 5 minutes. Filter the gel particles using a 0.8 μm microporous membrane to obtain the scar-removing gel matrix asiaticoside. The scar-removing gel matrix, containing asiaticoside, comprises the following raw materials: 22 parts hydroxypropyl-β-cyclodextrin, 1.8 parts asiaticoside modification solution, 16 parts corn oil, 45 parts Tween 80, 9 parts anhydrous ethanol, 9 parts propylene glycol, 0.08 parts polysorbate 20, 0.03 parts tert-butylhydroquinone, 41.2 parts gel matrix, 2.6 parts panthenol modification solution, and 0.1 parts ethylparaben.

[0053] The gel matrix was prepared by mixing carbomer 940, carbomer 980 and deionized water.

[0054] The mass ratio of Carbomer 940, Carbomer 980 and deionized water is 2.1:0.9:100.

[0055] The preparation of the asiaticoside modified solution includes the following steps: A1. Place asiaticoside, anhydrous lactose and maltol into an agate mortar and grind them at 350 rpm for 5 minutes to obtain a mixed powder. A2. Add deionized water and chitosan quaternary ammonium salt to the mixed powder and stir. Set the speed to 300 rpm and stir magnetically for 5 minutes to obtain the modified Centella asiatica extract.

[0056] The mass ratio of asiaticoside, anhydrous lactose, and maltol is 3:6:1.

[0057] The mass ratio of the mixed powder, deionized water, and chitosan quaternary ammonium salt is 1.5:2:0.3.

[0058] The chitosan quaternary ammonium salt is one of hydroxypropyltrimethylammonium chloride chitosan, carboxymethyltrimethylammonium chloride chitosan, and methyltrimethylammonium chloride chitosan.

[0059] The preparation of panthenol modified solution includes the following steps: panthenol, hydroxypropyl-α-cyclodextrin and γ-polyglutamic acid are added to a reaction vessel, deionized water is added and stirred, the stirring speed is set to 300 rpm and magnetic stirring is performed for 8 minutes to obtain panthenol modified solution.

[0060] The mass ratio of panthenol, hydroxypropyl-α-cyclodextrin, γ-polyglutamic acid, and deionized water was 4:8:1:10.

[0061] Add triethanolamine to adjust the pH to 6.3.

[0062] Example 3: A method for microemulsifying and encapsulating asiaticoside in a scar-reducing gel matrix. The method includes the following steps: S1. Hydroxypropyl-β-cyclodextrin was added to a reaction vessel, deionized water was added and stirred, the water bath temperature was set to 45℃, the stirring speed was 100 rpm, and the stirring was kept constant for 20 minutes. Centella asiatica glycoside modified solution was added and stirred, the temperature was set to 45℃, the stirring speed was 100 rpm, and the stirring was kept constant for 4 hours to obtain a mixture. The mixture was refrigerated, the temperature was set to 4℃, and the refrigeration was carried out for 15 minutes. The particles were filtered out using a 0.45μm microporous membrane to obtain an inclusion complex solution. S2. Mix corn oil, Tween 80, anhydrous ethanol and propylene glycol, set the water bath temperature to 37°C, the stirring speed to 500 rpm, and stir at a constant temperature for 15 minutes. Add the inclusion complex solution and stir, set the temperature to 37°C, the stirring speed to 500 rpm, and stir for 10 minutes. Add deionized water and stir, set the stirring speed to 800 rpm, and stir for 20 minutes. Add polysorbate 20 and tert-butylhydroquinone and stir, set the stirring speed to 600 rpm, and stir for 5 minutes to obtain asiaticoside microemulsion. S3. Add the asiaticoside microemulsion to the gel matrix and stir at 500 rpm for 15 minutes. Add the panthenol modification solution and stir at 500 rpm for 8 minutes. Add triethanolamine and stir at 300 rpm for 5 minutes. Add ethylparaben and stir at 300 rpm for 5 minutes. Filter the gel particles using a 0.8 μm microporous membrane to obtain the scar-removing gel matrix asiaticoside. The scar-removing gel matrix, containing asiaticoside, comprises the following raw materials: 24 parts hydroxypropyl-β-cyclodextrin, 1.9 parts asiaticoside modification solution, 18 parts corn oil, 47 parts Tween 80, 10 parts anhydrous ethanol, 10 parts propylene glycol, 0.09 parts polysorbate 20, 0.04 parts tert-butylhydroquinone, 42.4 parts gel matrix, 2.7 parts panthenol modification solution, and 0.11 parts ethylparaben.

[0063] The gel matrix was prepared by mixing carbomer 940, carbomer 980 and deionized water.

[0064] The mass ratio of Carbomer 940, Carbomer 980 and deionized water is 2.1:0.9:100.

[0065] The preparation of the asiaticoside modified solution includes the following steps: A1. Place asiaticoside, anhydrous lactose and maltol into an agate mortar and grind them at 400 rpm for 5 minutes to obtain a mixed powder. A2. Add deionized water and chitosan quaternary ammonium salt to the mixed powder and stir. Set the speed to 300 rpm and stir magnetically for 5 minutes to obtain the modified Centella asiatica extract.

[0066] The mass ratio of asiaticoside, anhydrous lactose, and maltol is 3:6:1.

[0067] The mass ratio of the mixed powder, deionized water, and chitosan quaternary ammonium salt is 1.5:2:0.3.

[0068] The chitosan quaternary ammonium salt is one of hydroxypropyltrimethylammonium chloride chitosan, carboxymethyltrimethylammonium chloride chitosan, and methyltrimethylammonium chloride chitosan.

[0069] The preparation of panthenol modified solution includes the following steps: panthenol, hydroxypropyl-α-cyclodextrin and γ-polyglutamic acid are added to a reaction vessel, deionized water is added and stirred, the stirring speed is set to 300 rpm and magnetic stirring is performed for 8 minutes to obtain panthenol modified solution.

[0070] The mass ratio of panthenol, hydroxypropyl-α-cyclodextrin, γ-polyglutamic acid, and deionized water was 4:8:1:10.

[0071] Add triethanolamine to adjust the pH to 6.5.

[0072] Comparative Example 1: The difference between this comparative example and Example 1 is that: Unmodified asiaticoside was used in this comparative example.

[0073] Comparative Example 2: The difference between this comparative example and Example 1 is that: Unmodified panthenol was used in this comparative example.

[0074] Comparative Example 3 differs from Example 1 in that: No gel matrix was used in this comparative example.

[0075] Performance testing: The microemulsion inclusion method of asiaticoside in scar-reducing gel matrix prepared in Examples 1, 2, 3, Comparative Examples 1, 2, and 3 was tested.

[0076] Performance testing: The relevant properties of the scar-removing gel matrix samples prepared by the microemulsion encapsulation method of asiaticoside in Examples 1-3 and Comparative Examples 1-3 were tested respectively, and the test data are recorded in Table 1 below:

[0077] Based on the above data, the following conclusions can be drawn: Among them, the scar smoothness improvement test was conducted using the pharmacodynamic test method of General Chapter 1144 of the Chinese Pharmacopoeia 2025, Example 1, Example 2, Example 3, Comparative Example 1, Comparative Example 2 and Comparative Example 3, which prepared the scar-removing gel matrix with the microemulsion inclusion method of Centella asiatica glycoside. The scar-removing gel matrix prepared using the transdermal absorption assay method in General Chapter 0913 of the Chinese Pharmacopoeia 2025 was tested for panthenol retention in the stratum corneum for 24 hours using the method described in Examples 1, 2, 3, Comparative Examples 1, 2, and 3. The cumulative release rate of the scar-reducing gel matrix prepared by the microemulsion inclusion method of Centella asiatica glycoside in the scar-reducing gel matrix prepared by Example 1, Example 2, Example 3, Comparative Example 1, Comparative Example 2 and Comparative Example 3 was tested in vitro for 24 hours using the release rate determination method in General Chapter 0931 of the Chinese Pharmacopoeia 2025.

[0078] Through the above demonstrations, the present invention is significantly superior to the control group in terms of improving scar smoothness, panthenol retention in the stratum corneum of the skin over 24 hours, and cumulative release rate over 24 hours of in vitro release, thus verifying the advanced nature and rationality of the preparation process.

[0079] In the description of this specification, references to terms such as "an embodiment," "example," "specific example," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the invention. In this specification, illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0080] The preferred embodiments of the present invention disclosed above are merely illustrative of the invention. These preferred embodiments do not exhaustively describe all details, nor do they limit the invention to the specific implementations described. Clearly, many modifications and variations can be made based on the content of this specification. This specification selects and specifically describes these embodiments to better explain the principles and practical applications of the invention, thereby enabling those skilled in the art to better understand and utilize the invention. The invention is limited only by the claims and their full scope and equivalents.

Claims

1. A method for microemulsifying and encapsulating asiaticoside in a scar-reducing gel matrix, characterized in that, The method for microemulsifying and encapsulating the scar-reducing gel matrix with asiaticoside includes the following steps: S1. Add hydroxypropyl-β-cyclodextrin to the reaction vessel, add deionized water and stir, add asiaticoside modified solution and stir to obtain a mixture, refrigerate the mixture, filter out particles using a filter membrane to obtain an inclusion complex solution; S2. Mix corn oil, Tween 80, anhydrous ethanol and propylene glycol, add inclusion complex solution and stir, add deionized water and stir, add polysorbate 20 and tert-butylhydroquinone and stir to obtain asiaticoside microemulsion. S3. Add the asiaticoside microemulsion to the gel matrix and stir, add panthenol modification solution and stir, add triethanolamine and stir, add ethylparaben and stir, filter out the gel particles using a filter membrane to obtain the scar-removing gel matrix asiaticoside. The scar-removing gel matrix, containing asiaticoside, comprises the following raw materials: 20-24 parts hydroxypropyl-β-cyclodextrin, 1.7-1.9 parts asiaticoside modification solution, 14-18 parts corn oil, 43-47 parts Tween 80, 8-10 parts anhydrous ethanol, 8-10 parts propylene glycol, 0.07-0.09 parts polysorbate 20, 0.02-0.04 parts tert-butylhydroquinone, 40-42.4 parts gel matrix, 2.5-2.7 parts panthenol modification solution, 0.09-0.11 parts ethylparaben, and the remainder being deionized water.

2. The method for microemulsifying and encapsulating asiaticoside in a scar-reducing gel matrix according to claim 1, characterized in that, The gel matrix is ​​prepared by mixing carbomer 940, carbomer 980 and deionized water.

3. The method for microemulsifying and encapsulating asiaticoside in a scar-reducing gel matrix according to claim 2, characterized in that, The mass ratio of Carbomer 940, Carbomer 980 and deionized water is 2.1:0.9:

100.

4. The method for microemulsifying and encapsulating asiaticoside in a scar-reducing gel matrix according to claim 1, characterized in that, The preparation of the asiaticoside modified solution includes the following steps: A1. Place asiaticoside, anhydrous lactose and maltol into an agate mortar and grind them to obtain a mixed powder; A2. Add deionized water and chitosan quaternary ammonium salt to the mixed powder and stir to obtain the modified solution of asiaticoside.

5. The method for microemulsifying and encapsulating the scar-removing gel matrix with asiaticoside according to claim 4, characterized in that, The mass ratio of asiaticoside, anhydrous lactose, and maltol is 3:6:

1.

6. The method for microemulsifying and encapsulating the scar-removing gel matrix with asiaticoside according to claim 4, characterized in that, The mass ratio of the mixed powder, deionized water, and chitosan quaternary ammonium salt is 1.5:2:0.

3.

7. The method for microemulsifying and encapsulating the scar-removing gel matrix with asiaticoside according to claim 4, characterized in that, The chitosan quaternary ammonium salt is one of hydroxypropyltrimethylammonium chloride chitosan, carboxymethyltrimethylammonium chloride chitosan, and methyltrimethylammonium chloride chitosan.

8. The method for microemulsifying and encapsulating asiaticoside in a scar-reducing gel matrix according to claim 1, characterized in that, The preparation of the panthenol modified solution includes the following steps: panthenol, hydroxypropyl-α-cyclodextrin and γ-polyglutamic acid are added to a reaction vessel, deionized water is added and stirred to obtain the panthenol modified solution.

9. The method for microemulsifying and encapsulating asiaticoside in a scar-reducing gel matrix according to claim 8, characterized in that, The mass ratio of panthenol, hydroxypropyl-α-cyclodextrin, γ-polyglutamic acid, and deionized water is 4:8:1:

10.

10. The method for microemulsifying and encapsulating asiaticoside in a scar-reducing gel matrix according to claim 1, characterized in that, The addition of triethanolamine adjusts the pH to 6-6.5.

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