Composition with synergistic effect of resisting acnes and photo-thermal damage and application of composition

Abstract

The invention relates to the technical field of skin care products, in particular to a composition with a synergistic effect of resisting acnes and photo-thermal damage and application. The composition is prepared from the following components: rhodomyrtus tomentosa fruit extract, dendrobium nobile stem extract, tephros glauca seed extract, verbascin root extract and cocoa seed fat, and the five components are synergistically regulated and controlled to synchronously realize dual effects of improving acne and relieving photo-thermal induced aging signs; through a multi-target and multi-layer action mechanism, collaborative intervention is performed from four key pathological links of regulating micro-ecological imbalance, blocking inflammation cascade reaction, repairing physical and biological barriers and neutralizing oxidative stress, a collaborative intervention network aiming at acne generation and photo-thermal aging is constructed, and the comprehensive effect from symptom improvement to root protection is achieved.

Description

Technical Field

[0001] This invention relates to the technical field of skincare products, specifically to a composition and its application that has synergistic effects in combating acne and photothermal damage. Background Technology

[0002] Acne, a common chronic inflammatory disease of the pilosebaceous unit, is influenced not only by internal factors but also by external environmental factors. Modern research has found that in addition to ultraviolet radiation from sunlight and high-energy blue light emitted by electronic devices, the local heat accumulation effect caused by wearing masks and protective gear in daily life can also exacerbate acne. These environmental stressors act on acne lesions through different mechanisms: photoradiation stimulates the sebaceous glands to produce large amounts of reactive oxygen species, triggering lipid peroxidation; while the local heat accumulation environment leads to increased skin temperature, increased sebum secretion, and creates an anaerobic environment conducive to the proliferation of Propionibacterium acnes. These factors collectively exacerbate the inflammatory response, leading to abnormal pigmentation and the formation of more difficult-to-resolve post-inflammatory hyperpigmentation (PIH). Simultaneously, photodamage and thermal damage synergistically disrupt the integrity of the skin barrier and alter the composition of the microbial community, creating favorable conditions for the occurrence and development of acne. For individuals with sensitive skin whose barrier function is already fragile, this synergistic damaging effect of multiple environmental factors on acne is particularly significant, requiring them to address the dual challenges of accelerated photoaging and thermal damage repair while treating acne, creating a complex and contradictory skincare dilemma.

[0003] Current skincare products for sensitive acne primarily focus on gentle cleansing, low-concentration acid peels, and physical sun protection, but rarely address the repair of heat damage. Existing methods have significant limitations: besides the potential irritation from chemical sunscreens and the tendency of physical sunscreens to clog pores, conventional skincare products lack targeted repair methods for localized heat accumulation damage caused by wearing masks, etc. More importantly, existing products often separate acne treatment from environmental protection, failing to recognize the synergistic deterioration relationship between light radiation, heat damage, and acne. In reality, acne-prone skin, due to its chronic inflammatory state, already has an imbalanced collagen metabolism. Continuous light and heat damage further accelerate matrix degradation, making effective environmental protection crucial while addressing acne. Ignoring these environmental factors, even if acne symptoms are temporarily controlled, it's difficult to avoid skin deterioration caused by environmental stress.

[0004] These challenges reflect the industry's urgent need for a new, multi-target, multi-level synergistic solution that can achieve the dual benefits of improving acne and reducing signs of photothermal aging, thereby meeting the market's pressing demand for products with comprehensive efficacy, from symptom improvement to root cause protection. Summary of the Invention

[0005] The purpose of this invention is to overcome the shortcomings of the prior art and provide a composition and its application for synergistic effects against acne and photothermal damage. The raw material components in the composition work synergistically to intervene in four key pathological links through a multi-target and multi-level mechanism of action: regulating microecological imbalance, blocking inflammatory cascade reactions, repairing physical and biological barriers, and neutralizing oxidative stress. This constructs a synergistic intervention network targeting acne occurrence and photothermal aging, achieving the dual effects of improving acne and reducing signs of photothermal aging.

[0006] To achieve the above objectives, the technical solution adopted by the present invention is as follows: In a first aspect, the present invention provides a composition for synergistic effects against acne and photothermal damage, comprising the following components: myrtle fruit extract, dendrobium stem extract, edamame seed extract, mullein root extract and cocoa seed butter, wherein the weight ratio of myrtle fruit extract, dendrobium stem extract, edamame seed extract, mullein root extract and cocoa seed butter is (1-8):(0.1-5):(0.01-1):(0.1-2):(0.01-1).

[0007] Myrtle fruit extract, as a precise regulator of the microecology and a polyphenolic antioxidant, has core active ingredients—gallic acid and ellagic acid—that specifically interfere with the biofilm formation of Propionibacterium acnes and inhibit its lipase activity, thereby reducing the stimulation of hair follicles by free fatty acids. In terms of antioxidation, its polyphenolic structure can directly terminate the lipid peroxidation chain reaction through a hydrogen donation mechanism, protecting the pilosebaceous unit from oxidative damage. Moreover, this extract achieves precise inhibition of pathogenic bacteria while avoiding widespread destruction of beneficial bacteria, which is key to targeted regulation of the microecology.

[0008] Dendrobium nobile stem extract serves as the core anti-inflammatory and immunomodulatory hub of the composition. Its active polysaccharides and phenanthrene compounds can target and inhibit the activation of key inflammatory signaling pathways such as nuclear factor-κB (NF-κB), significantly downregulate the overexpression of pro-inflammatory factors such as acne and UV-induced tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6), and rapidly control erythema and swelling. At the same time, its excellent moisturizing properties enhance skin hydration by upregulating the expression of aquaporin 3 (AQP3), which not only soothes dryness and discomfort, but also creates a stable colonization environment for probiotics on the skin surface, achieving dual stabilization at the immune and microecological levels.

[0009] The extract of soybean seed plays a dual role in regulating keratinocytes and repairing oxidative damage in the composition. Its unique flavonoids can not only effectively interfere with the formation of Propionibacterium acnes biofilm, but also inhibit the lipid peroxidation chain reaction through its strong antioxidant capacity, repairing DNA damage and functional abnormalities of keratinocytes caused by photoradiation and heat stress. By regulating the normal differentiation of keratinocytes at the hair follicle opening, the extract prevents keratin plugging problems induced by photothermal factors, blocking the synergistic deterioration of oxidative damage and acne from the source. In addition, this component can also significantly inhibit the overexpression of ROS-mediated matrix metalloproteinase-1 (MMP-1) and MMP-9, thereby protecting type I and type III collagen in the dermis from degradation. It is a component that combats collagen loss caused by photothermal aging.

[0010] As an active soothing and physical barrier strengthener, mullein root extract accelerates the completion of the epidermal differentiation terminal process by promoting the synthesis of key structural proteins such as filaggrin and loricrin in keratinocytes, thereby thickening and consolidating the "brick wall structure" of the stratum corneum. This process not only effectively reduces transepidermal water loss (TEWL) and repairs the physical barrier damaged by acne inflammation or photodamage, but also inhibits the excessive proliferation of harmful bacteria by maintaining a stable skin pH and low osmotic pressure environment, providing a structural basis for the balance of the skin microecology.

[0011] Cocoa seed butter, as a biomimetic lipid supplement and long-lasting barrier stabilizer, has a unique fatty acid composition (high content of oleic acid, stearic acid, and palmitic acid) that is highly similar to human sebum. It can instantly integrate into the sebum film and effectively repair the gaps in the lipid bilayer. In addition, as an excellent penetration enhancer, it can promote the transdermal absorption of other active ingredients. More importantly, it prevents compensatory sebum secretion caused by excessive dryness by continuously moisturizing, thus avoiding providing excessive "nutrients" to Propionibacterium acnes from the source and achieving a microecological balance between moisturizing and oil control.

[0012] Preferably, the weight ratio of the myrtle fruit extract, the Dendrobium nobile stem extract, the edamame seed extract, the mullein root extract, and the cocoa seed butter is (2-6):(0.5-3):(0.01-0.5):(0.5-1.5):(0.1-0.8).

[0013] More preferably, the weight ratio of the myrtle fruit extract, the Dendrobium nobile stem extract, the edamame seed extract, the mullein root extract and the cocoa seed butter is (3-5):(1-1.5):(0.1-0.5):(1-1.2):(0.2-0.5).

[0014] In a second aspect, the present invention provides the use of the composition described in the first aspect for synergistic effects against acne and photothermal damage in the preparation of skin care products.

[0015] Preferably, the skin care product is any one of toner, lotion, cream, mask or freeze-dried powder, and the amount of the composition added is 5%-10% of the total weight of the skin care product.

[0016] Thirdly, the present invention provides an emulsion comprising the following raw materials in weight percentages: 5%-10% of the composition described in the first aspect, 0.05%-0.5% of a thickener, 1%-10% of a humectant, 1%-6% of an emulsifier, 5%-15% of oils, 0.5%-3% of a preservative, and 0.01%-0.3% of a pH adjuster, with the balance being deionized water.

[0017] Preferably, the thickener includes at least one of xanthan gum, carbomer, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, ammonium acryloyldimethyl taurate / VP copolymer, and sclerotium gum.

[0018] Preferably, the moisturizer comprises at least one of allantoin, betaine, β-glucan, trehalose, caprylyl glycol, dipropylene glycol, sodium hyaluronate, 1,3-butanediol, glycerin, D-panthenol, and ceramide.

[0019] Preferably, the emulsifier comprises at least one of C14-22 alcohol, C12-20 alkyl glucoside, cetearyl glucoside, and sucrose stearate.

[0020] Preferably, the oil comprises at least one of caprylic / capric triglyceride, isononyl isononanoate, pentaerythritol tetraester, polydimethylsiloxane, stearyl alcohol, hydroxystearic acid, polymethylsilsesquioxane, and pentaerythritol distearate.

[0021] Preferably, the preservative includes at least one of 1,2-propanediol, 1,2-hexanediol, and p-hydroxyacetophenone.

[0022] Preferably, the pH adjuster includes at least one of arginine, tromethamine, and citric acid.

[0023] Fourthly, the present invention provides a method for preparing the emulsion described in the third aspect, comprising the following steps: S1. Mix the thickener and humectant evenly, add deionized water, heat and homogenize, keep warm for later use, to obtain component A; S2. Mix the oil and emulsifier, heat and stir until melted, keep warm for later use, to obtain component B; S3. After heating component A, add component B and homogenize to obtain emulsion base material; S4. Stir and cool the emulsified base material, add the components of the composition described in the first aspect, continue stirring until the material is uniform, then add the preservative and pH adjuster, stop stirring, discharge the material, and obtain the emulsion.

[0024] Preferably, the heating temperature in steps S1, S2, and S3 is 70-80℃.

[0025] Preferably, in step S4, the temperature is reduced to 30-50°C.

[0026] Compared with the prior art, the beneficial effects of the present invention are as follows: This invention provides a composition for synergistic anti-acne and anti-photothermal damage effects, effectively combining myrtle fruit extract, dendrobium stem extract, edamame seed extract, mullein root extract, and cocoa seed butter. These five components form a layered, mutually reinforcing "defense-regulation-repair" closed-loop system. Myrtle fruit extract and edamame seed extract synergistically inhibit lipid peroxidation, protecting cells from the source of oxidation. Cocoa seed butter and mullein root extract synergistically strengthen the skin barrier by replenishing lipids and synthesizing proteins, respectively. Dendrobium extract and mullein root extract enhance the vitality of dermal fibroblasts and keratinocytes, respectively, promoting... Tissue repair and renewal; Myrtle fruit extract and cocoa seed butter synergistically regulate the microecological basis from both "antibacterial" and "oil control" ends; Dendrobium nobile extract powerfully "anti-inflammatory" on this basis, blocking subsequent damage; Edamame seed extract, as the "antioxidant shield" of the whole system, resists photothermal aging from the source; while Mullein root extract is responsible for the final "barrier reinforcement", consolidating the curative effect and preventing recurrence; This composition, through multi-pathway synergy, synergistically intervenes in four key pathological links: regulating microecological imbalance, blocking inflammatory cascade reactions, repairing physical and biological barriers, and neutralizing oxidative stress, to achieve comprehensive treatment of the complex comorbidity of acne and photothermal aging. Attached Figure Description

[0027] Figure 1 The images show the improvement in acne in volunteers who used the lotion in Example 1 for 0, 14, and 28 days in Test Example 4. Detailed Implementation

[0028] To better illustrate the purpose, technical solution, and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0029] The sources of the raw materials used in the following examples and comparative examples are as follows: Myrtle fruit extract was purchased from GREENTECHS.A (Raylintech Co., Ltd.), under the trade name ACNILYS; The Dendrobium nobile stem extract was purchased from Shaanxi Yongyuan Biotechnology Co., Ltd., with the product name Dendrobium nobile extract and product number YYS-050. The edamame seed extract was purchased from Givaudan Fragrance & Flavor (Shanghai) Co., Ltd., under the trade name NEUROPHROLINE; The root extract of Verbena officinalis was purchased from Lai Yu Biotechnology (International) Group under the trade name LUMINESCINE; The cocoa butter was purchased from Nott-Shanghai Hongjiu Enterprise Development Co., Ltd., and its trade name is NL COCOA70.

[0030] Unless otherwise specified, all other materials and reagents used in the examples are commercially available.

[0031] The component composition (parts by weight) of the compositions for synergistic effects against acne and photothermal damage in Examples 1-7 of this invention is shown in Table 1 below. Each component was weighed according to the formula amount, mixed and homogenized to obtain each composition.

[0032] Table 1. Components and their weight parts of the compositions in Examples 1-7 Group Myrtle fruit extract (parts by weight) Dendrobium nobile stem extract (parts by weight) Edamame seed extract (parts by weight) Verbena officinalis root extract (parts by weight) Cocoa seed butter (parts by weight) Example 1 4 2 0.3 1 0.5 Example 2 3 1 0.1 1 0.2 Example 3 5 1.5 0.5 1.2 0.5 Example 4 2 0.5 0.01 0.5 0.1 Example 5 6 3 0.5 1.5 0.8 Example 6 1 0.1 0.01 0.1 0.01 Example 7 8 5 1 2 1 Comparative Example 1 The difference between Comparative Example 1 and Example 1 is that Comparative Example 1 does not add myrtle fruit extract, but uses Dendrobium nobile stem extract, Edamame seed extract, Verbena officinalis root extract and cocoa seed butter in a weight ratio of 2:0.3:1:0.5 to make up for the missing amount.

[0033] Comparative Example 2 The difference between Comparative Example 2 and Example 1 is that: no Dendrobium nobile stem extract was added in Comparative Example 2, and Myrtle fruit extract, Edamame seed extract, Verbena root extract and cocoa seed butter in a weight ratio of 4:0.3:1:0.5 were used to make up for the missing amount.

[0034] Comparative Example 3 The difference between Comparative Example 3 and Example 1 is that Comparative Example 3 does not add edamame seed extract, but uses myrtle fruit extract, dendrobium stem extract, mullein root extract and cocoa seed butter in a weight ratio of 4:2:1:0.5 to make up for the missing amount.

[0035] Comparative Example 4 The difference between Comparative Example 4 and Example 1 is that: in Comparative Example 4, no mullein root extract was added, and the missing amount was made up by using myrtle fruit extract, dendrobium stem extract, edamame seed extract and cocoa seed butter in a weight ratio of 4:2:0.3:0.5.

[0036] Comparative Example 5 The difference between Comparative Example 5 and Example 1 is that: no cocoa seed butter was added in Comparative Example 5, and the missing amount was made up by extracts of myrtle fruit, Dendrobium nobile stem, Edamame seed, and Mullein root in a weight ratio of 4:2:0.3:1.

[0037] Comparative Example 6 The difference between Comparative Example 6 and Example 1 is that Comparative Example 6 uses myrtle fruit extract, Dendrobium nobile stem extract, Edamame seed extract, Verbena officinalis root extract and cocoa seed butter in a weight ratio of 4:0.3:2:1:0.5.

[0038] The total weight of the compositions in Examples 1-7 and Comparative Examples 1-6 above is equal.

[0039] Test Example 1: Composition's ability to inhibit lipid peroxidation (LPO) and its anti-inflammatory properties. The core pathological process of acne is closely related to lipid peroxidation and inflammatory response. Lipids secreted by sebaceous glands undergo lipid peroxidation under the metabolism of Propionibacterium acnes and external environmental stress, producing lipid peroxides (LPOs) such as malondialdehyde. These peroxidation products not only directly damage the pilosebaceous unit but also act as inflammatory mediators, activating inflammatory pathways such as TLR2 / NF-κB, forming a vicious cycle of "oxidative stress-inflammatory amplification." Measuring LPO levels can assess the inhibitory effect of this composition on the core pathogenic link of acne—sebaceous peroxidation; simultaneously, detecting the expression levels of key inflammatory factors (such as TNF-α and IL-1β) can verify its effect in blocking the inflammatory cascade. These two indicators, from the dimensions of oxidative damage control and inflammatory pathway regulation, jointly demonstrate that this composition achieves fundamental symptom relief by breaking the vicious cycle of oxidation-inflammation in acne.

[0040] Samples: Compositions prepared in Examples 1-7 and Comparative Examples 1-6; 1.1 Assessing the ability to inhibit lipid peroxides (LPO) Experimental materials: Human sebaceous gland cells SZ95; penicillin-streptomycin antibiotic solution (Gibco); DMEM high glucose medium; lipid peroxide (LPO) ELISA kit; The test conditions were: incubator temperature 37±1℃, humidity 90±5%, carbon dioxide 5±1%; The testing method is as follows: (1) Resuscitation: The cell suspension was prepared at 2×10 3 Cells / well were seeded into 96-well cell culture plates, and 100 μL of DMEM high glucose medium containing 10% (v / v) fetal bovine serum and 1% penicillin-streptomycin solution was added to each well. The cells were cultured for 24 h. (2) Sample feeding: Discard the supernatant, wash 3 times with PBS buffer, add 100 μL of DMEM high glucose medium containing 10 mM hydrocortisone to each well of the control group, add 100 μL of DMEM high glucose medium containing 0.1% (v / v) of the composition and 10 mM hydrocortisone to each well of the sample group, set 3 replicates for each group, and continue to incubate for 24 h; (3) Detection of lipid peroxides (LPO): Lipid peroxides (LPO) were detected using an ELISA kit; (4) Calculate the LPO inhibition rate: The inhibitory effect of the sample on LPO is expressed as the LPO inhibition rate, which is calculated according to formula 1): LPO inhibition rate (%) = [1- (X 样品组 / X 对照组 )]×100% 1); In the formula: X 样品组 The lipid peroxide value of the sample group; X 对照组 The lipid peroxide value is for the control group. 1.2 Testing anti-inflammatory capabilities Experimental materials: RAW264.7 mouse macrophages were seeded in T75 culture flasks; DMEM medium; mouse tumor necrosis factor α (TNF-α) kit (Shanghai ELISA kit, catalog number ml002095); mouse interleukin 1β (IL-1β) ELISA kit (Shanghai ELISA kit, catalog number ml106733); The test conditions were: incubator temperature 37±1℃, humidity 90±5%, carbon dioxide 5±1%; The testing method is as follows: (1) Sample preparation: The compositions in the examples and comparative examples were dissolved in DMSO and then diluted with DMEM medium to a final experimental concentration of 0.1% (v / v). (2) Resuscitation of RAW264.7 cells: The cell suspension was seeded into 96-well cell culture plates at a density of 2.0 × 10⁶ cells per well. 4 Add 100 μL of DMEM medium containing 10% (v / v) fetal bovine serum and 1% penicillin-streptomycin solution to each well and revive and culture for 24 h; (3) Modeling: Discard the supernatant, wash 3 times with PBS buffer, divide the cells into model group and sample group, and set 3 replicates for each group; add 100 μL of DMEM medium containing 1 µg / mL LPS to each well of the model group and sample group for pretreatment for 12 h, discard the supernatant, wash 3 times with PBS buffer, and establish an inflammation model; (4) Sample feeding: Add 100 μL of DMEM medium to each well of the model group; add 100 μL of medium containing 0.1% of the corresponding composition to each well of the sample group, culture for 2 h, collect cells from each group, discard the medium, wash twice with PBS buffer; add 100 μL of TRIzol lysis buffer to each well, scrape the cells, transfer them to EP tubes, and store at -80℃ for later use. (5) Protein content detection by ELISA: The levels of inflammatory factors such as TNF-α and IL-1β in RAW264.7 cells were detected by mouse tumor necrosis factor α (TNF-α) kit and mouse interleukin 1β (IL-1β) ELISA kit, respectively. (6) Calculate the TNF-α inhibition rate and IL-1β inhibition rate according to formula 2); Inhibition rate = [1 - (X)] 样品组 / X 模型组 )] × 100% 2); In the formula: X 样品组 The content of TNF-α or IL-1β in the sample group; X 模型组 The TNF-α or IL-1β content in the model group; The greater the inhibition rate of the inflammatory factors, the stronger the anti-inflammatory ability of the composition; the calculated results are shown in Table 2. Table 2. Data on the inhibitory effect of lipid peroxides (LPO) and anti-inflammatory activity. Group LPO inhibition rate (%) TNF-α inhibition rate (%) IL-1β inhibition rate (%) Example 1 68.2 83.6 85.1 Example 2 65.8 80.5 83.7 Example 3 66.3 81.2 84.6 Example 4 63.4 77.8 81.4 Example 5 64.1 79.0 80.5 Example 6 60.5 76.3 77.9 Example 7 61.7 75.4 78.8 Comparative Example 1 41.2 53.1 55.3 Comparative Example 2 44.1 48.9 50.6 Comparative Example 3 43.9 55.7 56.1 Comparative Example 4 45.6 56.3 58.7 Comparative Example 5 47.3 58.6 60.2 Comparative Example 6 59.8 74.1 76.3 Test Example 2: Test on the ability of the composition to inhibit the growth of Propionibacterium acnes The biofilm of Propionibacterium acnes is its core barrier against external attacks and for maintaining pathogenicity. This structure significantly enhances bacterial drug resistance by forming a physical protective layer and continuously stimulates inflammatory responses. By verifying the inhibitory ability of the composition on biofilm formation, it can be demonstrated that it can break through the core defense mechanism of bacteria and directly undermine the microecological advantage of Propionibacterium acnes. This biofilm-targeting intervention strategy not only effectively blocks bacterial colonization and reproduction but also controls acne development from the source.

[0041] This study investigates the inhibitory effect of the compositions prepared in Examples 1-7 and Comparative Examples 1-6 on Propionibacterium acnes.

[0042] The test method is as follows: Using the 96-well plate crystal violet staining method, each composition was added to a solution with a concentration of 5 × 10⁻⁶. 5The final drug concentration was 200 µg / mL in a bacterial culture containing cfu / mL. The culture was anaerobic at 37℃ for 6 days. The upper layer of planktonic cells was discarded, and the cells were washed with sterile water and fixed with formaldehyde for 10 min. After removal, the cells were air-dried for 5 min. The biofilm was stained with 0.1% crystal violet for 20 min. After removing the dye, 95% (v / v) ethanol solution was added for decolorization for 15 min. 100 µL of the decolorized solution was added to a blank plate, and the absorbance was measured at 570 nm using an ELISA reader. The percentage of biofilm formation in each experimental group was calculated with the biofilm of the control group without the added composition as 100%. The results are shown in Table 3.

[0043] The inhibitory rate of the composition against Propionibacterium acnes is demonstrated by inhibiting the formation of the Propionibacterium acnes biofilm. The less biofilm formed, the better the inhibition of the composition against Propionibacterium acnes.

[0044] Table 3 Biofilm formation rate data Group Biofilm formation rate (%) Example 1 56.31 Example 2 59.87 Example 3 58.92 Example 4 61.14 Example 5 63.05 Example 6 65.89 Example 7 64.68 Comparative Example 1 89.23 Comparative Example 2 86.16 Comparative Example 3 84.02 Comparative Example 4 83.77 Comparative Example 5 81.24 Comparative Example 6 70.83 The data in Tables 2-3 regarding the LPO inhibition rate, TNF-α inhibition rate, IL-1β inhibition rate, and biofilm formation rate of Examples 1 and Comparative Examples 1-5 show that the composition prepared by combining five components—myrtle fruit extract, Dendrobium nobile stem extract, Edamame seed extract, Verbena root extract, and cocoa seed butter—significantly improved the LPO inhibition rate, TNF-α inhibition rate, and IL-1β inhibition rate, and significantly reduced the biofilm formation rate compared to the composition prepared by combining any four of these components. This indicates that the five components—myrtle fruit extract, Dendrobium nobile stem extract, Edamame seed extract, Verbena root extract, and cocoa seed butter—have a synergistic effect.

[0045] Based on the data on LPO inhibition rate, TNF-α inhibition rate, IL-1β inhibition rate, and biofilm formation rate of Examples 1-7 and Comparative Example 6 in Tables 2-3, it can be concluded that the synergistic effect of the composition is better when the weight ratio of Myrtle Fruit Extract, Dendrobium nobile Stem Extract, Edamame Seed Extract, Verbena Root Extract, and Cocoa Seed Butter is (1-8):(0.1-5):(0.01-1):(0.1-2):(0.01-1); and the composition has the best effect when the weight ratio of Myrtle Fruit Extract, Dendrobium nobile Stem Extract, Edamame Seed Extract, Verbena Root Extract, and Cocoa Seed Butter is 4:2:0.3:1:0.5.

[0046] Application Example 1-7 and Comparative Application Example 1-7 The compositions of Examples 1-7 and Comparative Examples 1-6 were added to the emulsion at a concentration of 10 wt% to obtain the emulsions of Application Examples 1-7 and Comparative Application Examples 1-6. The formulations are shown in Table 4.

[0047] The methods for preparing emulsions in Application Examples 1-7 and Comparative Application Examples 1-6 include the following steps: The method for preparing an emulsion includes the following steps: S1. Mix the thickener and humectant evenly, add deionized water, heat to 80°C and homogenize, keep warm for later use, to obtain component A; S2. Mix the oil and emulsifier, heat and stir at 80°C until melted, keep warm for later use, and obtain component B; S3. Heat component A to 80°C, add component B, homogenize, and obtain emulsion base material; S4. Stir the emulsified base material and cool it to 40°C. Add the components of the composition and continue stirring until the material is uniform. Then add the preservative and pH adjuster, stop stirring, and discharge the material to obtain the emulsion.

[0048] Table 4. Emulsion formulations of Application Examples 1-7 and Comparative Application Examples 1-6

[0049] Compare with application example 7 (blank application example) The difference between Comparative Application Example 7 and Application Example 1 is that the emulsion in Comparative Application Example 7 does not contain the composition, but uses an equal amount of deionized water instead of the composition, and the preparation method is the same as in Application Example 1.

[0050] Test Example 3: Human Skin Patch Test To rigorously verify the high safety and gentleness of the emulsion prepared using the composition of this application for sensitive skin, this study designed and implemented an enhanced human occlusive patch test.

[0051] The trial recruited 150 volunteers aged 20 to 50 (50% of whom reported having sensitive skin), with an equal number of men and women, and randomly divided into 15 groups of 10 each. All volunteers signed informed consent forms. To simulate the common barrier damage state of sensitive skin, medical 3M tape was used to repeatedly peel off the test area on the flexor surface of the volunteers' arms five times to create a more stringent testing environment. Subsequently, 0.020 mL of test samples (the lotions prepared in Application Examples 1-7 and Control Application Examples 1-7) and purified water (negative control group) were placed in 10-well patch applicators and applied to the pretreated skin with hypoallergenic tape for 24 hours. At 0, 24, and 48 hours after removing the patch applicators, professional assessors observed and recorded skin reactions according to the adverse reaction grade (see Table 5). The final safety assessment criterion was: if the average irritation score of the application groups was not higher than that of the blank control group, and no adverse reactions of grade 2 or above occurred, the product was deemed safe and non-irritating and suitable for sensitive skin.

[0052] Table 5. Adverse skin reaction levels Rating levels Skin reaction 0 negative reaction 1 Suspicious reaction, only slight erythema 2 Weak positive reaction (erythema reaction): erythema, infiltration, edema, and papules may be present. 3 Strong positive reaction (herpes reaction): erythema, infiltration, edema, papules; the reaction may extend beyond the test area. 4 Extremely strong positive reaction (confluent herpes simplex reaction): obvious erythema, severe infiltration, edema, confluent herpes simplex; reaction extends beyond the test area. The results of the human skin patch test showed that the skin reactions of volunteers in the application examples 1-7 and the control examples 1-7 were observed at 0 h, 24 h and 48 h. No adverse skin reactions were observed in any of them, and all were negative reactions. This indicates that the emulsions prepared by the composition provided by the present invention are safe and non-irritating to human skin.

[0053] Test Example 4: Human Efficacy Test Experimental Methods: Based on the principles of human efficacy evaluation in the "Cosmetic Safety Technical Specifications" (2015 edition), this experiment aims to scientifically evaluate the emulsions corresponding to Application Examples 1-7 and compare the emulsions prepared using Application Examples 1-7, and to conduct safety and efficacy tests in the following aspects. Volunteer Selection Criteria: Volunteers with mild facial acne (e.g., ≤10 papules or pustules) and independent acne scars ≥3mm in diameter are eligible. Daily electronic device use must exceed 4 hours / day or prolonged mask-wearing is required. Transdermal water loss (TEWL) must be ≥20 g / m²·h (measured using Tewameter™ Hex). Exclusion Criteria: Recent (within 1 month) use of hormonal medications, anti-acne drugs, or facial cosmetic treatments; allergy to any component of the test sample; pregnant or breastfeeding women. Grouping and Number of Participants: The 70 eligible volunteers were randomly divided into 14 groups (Application Examples 1-7 and Comparative Application Examples 1-7), with 5 people in each group.

[0054] Test samples: Emulsions prepared in Application Examples 1-7 and Comparative Application Examples 1-7 (double-blind method numbering), wherein the emulsion prepared in Comparative Application Example 7 served as the blank control group, and the remaining application examples and comparative application examples served as the sample groups.

[0055] Instructions for use: After cleansing in the morning and evening, volunteers should take 1mL of the sample and apply it evenly to the entire face, gently massaging until fully absorbed. Continue using for 28 days (D0, D14, D28). The condition of the facial skin after 28 days of product use will be tested to comprehensively evaluate the product's efficacy in acne removal, redness reduction, and repair. Testing: Volunteers in each group underwent instrument testing on day 0 (D0 baseline period), day 14 (D14), and day 28 (D28 trial endpoint); Preparation before testing: a) After the subject arrives, cleanse the face with a uniform, non-irritating facial cleanser; b) Rest quietly for 30 minutes in a constant temperature and humidity environment (temperature 21±1℃, humidity 50±10%); c) Keep eyes closed and relaxed during the test to avoid facial expressions and movements that may interfere with the test. Test indicators: a) Acne-removing efficacy: Facial acne was quantified and photographed using the VISIA-CR facial image analyzer, and the number of acne lesions was counted; b) Repair and redness-reducing efficacy: The TEWL value of the skin surface and facial erythema were measured using a skin moisture loss tester (Tewameter™ Hex) and MX 18, respectively, to evaluate the skin barrier repair effect and redness-reducing effect; c) Anti-aging efficacy: The improvement of fine lines under the eyes was detected using Visia to evaluate the anti-photoaging effect of the composition. The calculation formulas for each test indicator are as follows: TEWL Improvement Rate (%) = (TEWL D0 -TEWL D14或28 ) / TEWL D0 ×100%; In the formula: TEWL D0 This is the TEWL data for day 0; TEWL D14或D28 TEWL data for day 14 or day 28; Acne improvement rate (%) = (acne D0 Acne D14或D28 Acne D0 ×100%; In the formula: Acne D0 This is the acne data for day 0; Acne D14或D28 Acne data for day 14 or day 28; Fine line improvement rate (%) = (fine lines D0 Fine lines D14或D28 Fine lines D0 ×100%; In the formula: Fine lines D0 This is the fine line data for day 0; Fine lines D14或D28 Data on fine lines on day 14 or day 28; Erythema improvement rate (%) = (erythema) D0 -erythema D14或D28 ) / erythema D0 ×100%; In the formula: erythema D0 Erythema data for day 0; erythema D14或D28 Erythema data for day 14 or day 28; The calculation results are averaged, as shown in Table 6.

[0056] Table 6. Human efficacy test data for each group of emulsions

[0057] Table 6 shows the TEWL improvement rate, acne improvement rate, fine line improvement rate, and erythema improvement rate of Application Example 1 and Comparative Application Examples 1-5 and 7. It can be seen that the emulsion prepared using a combination of five ingredients—myrtle fruit extract, Dendrobium nobile stem extract, Edamame seed extract, Verbena root extract, and cocoa seed butter—has a high TEWL improvement rate, acne improvement rate, fine line improvement rate, and erythema improvement rate. This indicates that the five ingredients—myrtle fruit extract, Dendrobium nobile stem extract, Edamame seed extract, Verbena root extract, and cocoa seed butter—work synergistically, enhancing the acne-removing and repairing abilities of the composition.

[0058] Based on the data of TEWL improvement rate, acne improvement rate, fine line improvement rate, and erythema improvement rate in Application Examples 1-7 and Comparative Application Example 6 in Table 6, it can be concluded that the synergistic effect of the composition is better when the weight ratio of myrtle fruit extract, Dendrobium nobile stem extract, Edamame seed extract, Verbena root extract, and cocoa seed butter is (1-8):(0.1-5):(0.01-1):(0.1-2):(0.01-1); and the composition has the best effect when the weight ratio of myrtle fruit extract, Dendrobium nobile stem extract, Edamame seed extract, Verbena root extract, and cocoa seed butter is 4:2:0.3:1:0.5.

[0059] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A composition for anti-acne, anti-photo-damage synergistic efficacy, characterized in that, The composition comprises the following components: Myrica rubra extract, Dendrobium stem extract, Millettia pinnata seed extract, Verbascum thapsus root extract and Theobroma seed butter, and the weight ratio of the Myrica rubra extract, the Dendrobium stem extract, the Millettia pinnata seed extract, the Verbascum thapsus root extract and the Theobroma seed butter is (1-8):(0.1-5):(0.01-1):(0.1-2):(0.01-1).

2. The composition for anti-acne anti-photothermal damage synergistic efficacy according to claim 1, wherein, The weight ratio of the Myrica rubra extract, the Dendrobium stem extract, the Millettia pinnata seed extract, the Verbascum thapsus root extract and the Theobroma seed butter is (2-6):(0.5-3):(0.01-0.5):(0.5-1.5):(0.1-0.8).

3. The composition for anti-acne anti-photothermal damage synergistic efficacy according to claim 1, wherein The weight ratio of the Myrica rubra extract, the Dendrobium stem extract, the Millettia pinnata seed extract, the Verbascum thapsus root extract and the Theobroma seed butter is (3-5):(1-1.5):(0.1-0.5):(1-1.2):(0.2-0.5).

4. Use of the composition for anti-acne and anti-photo-thermal damage synergistic efficacy according to any one of claims 1-3 in the preparation of a skin care product.

5. Use of a composition for anti-acne, anti-photothermal damage synergistic efficacy according to claim 4, for the preparation of a skin care product, characterized in that, The skin care product is any one of a toner, a lotion, a cream, a mask or a lyophilized powder, and the added amount of the composition is 5%-10% of the total weight of the skin care product.

6. An emulsion characterized in that, The raw materials include the following weight percentages: 5%-10% of the composition according to any one of claims 1-3, 0.05%-0.5% of a thickening agent, 1%-10% of a humectant, 1%-6% of an emulsifier, 5%-15% of an oil, 0.5%-3% of a preservative and 0.01%-0.3% of a pH adjuster, and the balance is deionized water.

7. The emulsion of claim 6, wherein, The raw materials are selected from at least one of (a)-(f): (a) the thickening agent includes at least one of xanthan gum, carbomer, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, ammonium acryloyldimethyl taurate / VP copolymer and arthrobium gum; (b) the humectant includes at least one of allantoin, betaine, beta-glucan, trehalose, caprylyl glycol, dipropylene glycol, sodium hyaluronate, 1,3-butanediol, glycerol, D-panthenol and ceramide; (c) the emulsifier includes at least one of C14-22 alcohol, C12-20 alkyl glucoside, cetyl stearyl glucoside and sucrose stearate; (d) the oil includes at least one of caprylic / capric triglyceride, isononyl isononanoate, pentaerythrityl tetraester, dimethicone, stearyl alcohol, hydroxystearic acid, polymethylsilsesquioxane and pentaerythrityl distearate; (e) the preservative includes at least one of 1,2-propanediol, 1,2-hexanediol and p-hydroxyacetophenone; (f) the pH adjuster includes at least one of arginine, tromethamine and citric acid.

8. A process for the preparation of an emulsion as claimed in claim 6 or 7, characterised in that, The method comprises the following steps: S1, uniformly mix the thickening agent with the humectant, add deionized water, homogenize after heating, and keep warm for standby, to obtain component A; S2, mix the oil with the emulsifier, heat and stir until melted, and keep warm for standby, to obtain component B; S3, after heating component A, add component B, and homogenize, to obtain an emulsified base; S4, stirring and cooling the emulsifying base, adding each component of the composition according to any one of claims 1-3, continuing to stir until the material is uniform, then adding a preservative and a pH regulator, stopping stirring, discharging, and obtaining the emulsion.

9. The production method according to claim 8, wherein The heating temperature in the steps S1, S2, and S3 is 70-80°C.

10. The production method according to claim 8, wherein The cooling temperature in the step S4 is 30-50°C. The cooling temperature in the step S4 is 30-50°C.

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