Multifunctional array ultrasonic driving particle focusing system and method thereof

Abstract

The invention discloses a multifunctional array ultrasonic driving particle focusing system and a method thereof. The system comprises a particle focusing module with a sample channel and an ultrasonic transducer array which is arranged on the particle focusing module and at least comprises two piezoelectric transducer units, and the ultrasonic transducer array receives through ultrasonic driving of multiple channels; when the piezoelectric transducer is used as a transmitting end, a stable sound energy potential well is generated in the sample channel; the passing particles are stably gathered to the lowest potential energy position through the sound energy potential well; the sound field generated by each pair of piezoelectric transducers is a one-dimensional sound field, and particles are gathered at the center of the flow channel after passing through the sound fields generated by at least two pairs of piezoelectric transducers. When particles are focused, the frequency of generating the maximum acoustic potential energy difference in the sample channel can be monitored in real time through multi-channel ultrasonic driving receiving, frequency feedback is made, and the particle focusing precision and sensitivity are guaranteed.

Description

Technical Field

[0001] This invention relates to an ultrasonic-driven particle focusing system, which relates to the fields of microfluidics and acoustic manipulation technology, and specifically to a multifunctional array ultrasonic-driven particle focusing system and method thereof. Background Technology

[0002] In fields such as biomedical engineering, clinical diagnostics, new drug development, and environmental monitoring, the precise and efficient sorting, enrichment, capture, and arrangement of microscopic targets (such as cells, viruses, bacteria, DNA molecules, and microplastic particles) in liquid samples is a crucial fundamental technology. Among numerous micromanipulation technologies, acoustic fluid manipulation, which utilizes the interaction between ultrasound and microfluidics to manipulate particles, is gaining increasing popularity due to its outstanding advantages, including non-contact operation, minimal damage to biological samples (especially living cells), label-free operation, and system integrability.

[0003] A typical application that heavily relies on precise particle focusing is flow cytometry. A flow cytometer is a high-throughput detection tool capable of rapid, multi-parameter quantitative analysis and sorting of cells or other microparticles suspended in a fluid. To ensure accuracy and high resolution, a core prerequisite is that the analyte particles must be arranged in a precise single column within the microfluidic channel—a "single-cell flow"—ensuring that each particle passes through the laser detection point at the same speed and position. Traditional flow cytometry primarily relies on hydrodynamic focusing, using a high-speed "sheath fluid" to compress the sample flow from the periphery, confining it to the center of the channel. However, this method has inherent drawbacks, including consuming large amounts of expensive sheath fluid, diluting the sample, and reducing focusing effectiveness at high throughput. Therefore, acoustic focusing, as an alternative or auxiliary technique that eliminates or significantly reduces sheath fluid dependence, has become an important direction for development in this field.

[0004] However, existing acoustic focusing technologies still have significant limitations in practical applications, especially when meeting the requirements of complex systems such as high-performance flow cytometers. These technologies typically employ a fixed-function design approach, achieving specific manipulation purposes through carefully designed, fixed structures. For example, the technology disclosed in patent CN106807459B achieves two-dimensional focusing by setting fixed longitudinal and transverse transducers above and to the sides of the microchannel; another example is patent CN207336317U, which uses two independent transducers to perform focusing and sorting tasks respectively.

[0005] However, ultrasonic manipulation based on the resonance principle is extremely sensitive to the operating frequency. To achieve efficient focusing, the system must operate at a precise system resonant frequency. However, in actual operation, the heat generation effect of the piezoelectric transducer itself, as well as the dynamic changes in the temperature, composition, or flow rate of the fluid sample, will cause changes in the sound velocity and geometry within the microfluidic cavity, resulting in a real-time "drift" of the optimal resonant frequency. Existing technologies typically use a fixed driving frequency. Once frequency drift occurs, the preset frequency and the actual optimal frequency become mismatched, leading to a sharp decrease in the intensity of the acoustic standing wave field, a deterioration in the focusing effect, or even complete failure. These systems generally lack real-time monitoring capabilities for the chip's acoustic state and corresponding frequency feedback correction mechanisms, making them open-loop control systems. Their stability and reliability are difficult to guarantee when operating continuously for extended periods or dealing with complex and variable samples.

[0006] Therefore, there is an urgent need in this field for a stable particle manipulation technology to break through the existing open-loop control design concept and instead adopt a sound field manipulation method that can be programmed in real time via electrical signals and has adaptive adjustment capabilities. Summary of the Invention

[0007] To address the problems existing in the background art, this invention provides a multifunctional array ultrasonic-driven particle focusing system and method. This invention develops a universal method capable of flexibly performing efficient focusing tasks on a single, highly integrated device according to requirements, and capable of real-time monitoring of system status to compensate for frequency drift and ensure long-term stable and efficient operation. This provides a more efficient, universal, and reliable microscopic manipulation platform for fields such as life sciences, medical diagnostics, and materials science.

[0008] The technical solution adopted in this invention is: I. A multifunctional array ultrasound-driven particle focusing system: The particle focusing system includes an ultrasonic transducer array and a particle focusing module with a sample channel. The ultrasonic transducer array includes at least two piezoelectric transducer units, which are arranged sequentially along the length of the particle focusing module on the outer wall of the particle focusing module. Each piezoelectric transducer unit includes a pair of independently controlled piezoelectric transducers, and each pair of piezoelectric transducers is arranged opposite each other on the outer wall of the particle focusing module. Each pair of piezoelectric transducers is divided and constructed into a first focusing subset and a second focusing subset. The first focusing subset and the second focusing subset are driven and controlled at different times in a time-multiplexed manner, so that the first focusing subset and the second focusing subset work in driving mode and monitoring mode, thereby controlling the particle focusing system to switch the focusing driving mode and periodically repeat the scanning-monitoring-locking process to realize ultrasonic-driven particle focusing of the particle fluid in the sample channel.

[0009] The piezoelectric transducers in the ultrasonic transducer array are evenly spaced along the circumference of the particle focusing module, that is, each piezoelectric transducer is attached around the outer wall of the particle focusing module and does not overlap in the circumferential direction; the two piezoelectric transducer units in each piezoelectric transducer unit are symmetrically distributed about the axis of the particle focusing module; the piezoelectric transducer units are staggered along the length of the particle focusing module, and the positions of two adjacent piezoelectric transducer units in the length direction can be spaced apart or overlapped, and each piezoelectric transducer unit does not need to cover the entire length of the particle focusing module.

[0010] II. A particle focusing method for a multifunctional array ultrasound particle focusing module, comprising: After particle fluid is introduced into the sample channel of the particle focusing module, a first driving signal is output to each piezoelectric transducer in the first focusing subset of the ultrasonic transducer array. The first focusing subset is in driving mode to generate an acoustic field in the sample channel for manipulating the particle fluid and performing a scanning process. Then, feedback signals are received from each piezoelectric transducer in the second focusing subset for monitoring. The second focusing subset is in monitoring mode. The feedback signal is related to the acoustic characteristic parameters of the sample channel and is used to characterize the current optimal resonant frequency of the sample channel. Based on the feedback signal, the optimal resonant frequency is determined, and the frequency of the first driving signal is dynamically adjusted to match or track the optimal resonant frequency. Then, the ultrasonic transducer array switches to focusing driving mode, outputs a second driving signal to each piezoelectric transducer in both the first and second focusing subsets, and locks them. Both the first and second focusing subsets are in driving mode, and then the scanning-monitoring-locking process is repeated periodically to achieve ultrasonic-driven particle focusing.

[0011] The scanning of the first driving signal is a range scan, and the scanning frequency of the range scan is the preset center resonant frequency f. c The scan frequency is ±500kHz, supplemented by 1-10kHz.

[0012] After receiving the feedback signal, each piezoelectric transducer in the second focusing subset determines the sound pressure amplitude in the sample channel. When the first driving signal is scanned, the maximum value of the sound pressure amplitude is determined, and the frequency at which the sound pressure amplitude is at its maximum is taken as the optimal resonant frequency f. opt .

[0013] The frequency of the second driving signal is the optimal resonant frequency f. opt Linear scan drive within the range of ±1-50kHz, not driven at a single frequency, with a single frequency drive time of 1-100ms.

[0014] The phase adjustment range of the first driving signal and the second driving signal is -180° to 180°, and the amplitude adjustment range is 0 to 30V.

[0015] The ultrasonic transducer array of this invention uses a multi-channel ultrasonic drive and receiver. When the piezoelectric transducer is used as the transmitter, it can generate a stable acoustic potential well in the sample channel. The acoustic potential well contains a high and low potential energy difference, which can stably gather the passing particles to the lowest potential energy point. The sound field generated by each pair of piezoelectric transducers is a one-dimensional sound field. After passing through the sound field generated by at least two pairs of piezoelectric transducers, the particles can gather at the center of the flow channel. Each pair of piezoelectric transducers contains at least one transceiver material. Before, during and after particle focusing, the frequency of the maximum acoustic potential energy difference generated in the sample channel can be monitored in real time by the multi-channel ultrasonic drive and receiving circuit, and frequency feedback can be made to ensure the particle focusing accuracy and sensitivity.

[0016] The beneficial effects of this invention are: This invention divides the transducer array into a driving unit and a monitoring unit, and introduces closed-loop feedback control, which can monitor and compensate for the resonant frequency drift caused by factors such as temperature in real time, ensuring the stability and efficiency of particle manipulation effect under long-term continuous operation.

[0017] In this invention, since the system can always maintain the optimal operating frequency, its sound field energy conversion efficiency and the force on particles are guaranteed, thereby enabling more accurate and efficient particle focusing. It is especially suitable for applications with extremely high requirements for stability and accuracy, such as high-performance flow cytometry analysis.

[0018] This invention enables efficient ultrasonic particle focusing to concentrate a particle stream into a smaller area. Furthermore, through the combination of transducer arrays, stable particle focusing can still be achieved under high-speed flow, significantly increasing sample throughput and realizing high-throughput particle focusing.

[0019] The particle focusing system driven by array ultrasound in this invention can be well integrated with existing flow cytometers, and has good versatility and compatibility. Attached Figure Description

[0020] Figure 1 This is a system framework diagram of the present invention; Figure 2 This is a schematic diagram of the 4-array particle focusing system of the present invention; Figure 3 This is a schematic diagram of the 6-array particle focusing system of the present invention; Figure 4 This is a simulation diagram of the focusing effect of the 4-array particle focusing system of the present invention; Figure 5 This is a diagram showing the relationship between frequency scanning and received voltage signal according to the present invention; Figure 6 This is a graph showing the relationship between frequency and focus width in this invention; Figure 7 This is a comparison diagram of the focusing width between single-frequency drive and frequency sweep drive of the present invention; In the figure: 10, sample channel; 20, ultrasonic transducer array; 30, particle focusing module. Detailed Implementation

[0021] The present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0022] like Figure 1 As shown, the multifunctional array ultrasonic-driven particle focusing system of the present invention includes an ultrasonic transducer array 20 and a particle focusing module 30 with a sample channel 10. The ultrasonic transducer array 20 includes at least two piezoelectric transducer units, which are arranged sequentially on the outer side wall of the particle focusing module 30 along the length direction of the particle focusing module 30. The ultrasonic transducer array 20 is connected to the particle focusing module 30 by epoxy resin. Each piezoelectric transducer unit includes a pair of independently controlled piezoelectric transducers, and each pair of piezoelectric transducers is arranged opposite each other on the outer side wall of the particle focusing module 30. Each pair of piezoelectric transducers is divided and constructed into a first focusing subset and a second focusing subset. The first focusing subset and the second focusing subset are driven and controlled at different times in a time-multiplexed manner, so that the first focusing subset and the second focusing subset work in a driving mode and a monitoring mode, so as to control the particle focusing system to switch the focusing driving mode and periodically repeat the scanning-monitoring-locking process to realize ultrasonic-driven particle focusing of the particle fluid in the sample channel 10.

[0023] The piezoelectric transducers in the ultrasonic transducer array 20 are evenly spaced along the circumference of the particle focusing module 30, meaning each piezoelectric transducer is attached around the outer wall of the particle focusing module 30 and does not overlap in the circumferential direction; the two piezoelectric transducer units in each unit are symmetrically distributed about the axis of symmetry of the particle focusing module 30; the piezoelectric transducer units are staggered along the length of the particle focusing module 30, and the positions of adjacent piezoelectric transducer units can be spaced apart or overlapped in the length direction, and each piezoelectric transducer unit does not need to cover the entire length of the particle focusing module 30. Figure 2 As shown, this is a four-array ultrasonic transducer array 20, with four piezoelectric transducers arranged at 90° intervals on the cross-section of sample channel 10. Figure 3 As shown, this is a six-array particle focusing system, in which six piezoelectric transducers are placed at 60° intervals on the cross section of sample channel 10.

[0024] The spacing between each piezoelectric transducer unit in the ultrasonic transducer array 20 along the length of the particle focusing module 30 does not exceed 1 mm. The width of the piezoelectric transducer is 3-8 mm, and the length is 5-50 mm. The total length of the piezoelectric transducers along the length of the particle focusing module 30 is 10-150 mm, preferably 100 mm.

[0025] The ultrasonic transducer array 20 is ultrasonically controlled by at least four channels, each channel controlling one piezoelectric transducer, with at least half of the channels capable of receiving ultrasonic signals. The input voltage of the piezoelectric transducer is 10-50V, preferably 25V. The operating frequency of the piezoelectric transducer is ±500kHz, centered on the frequency corresponding to half the wavelength of the sound wave in water corresponding to the size of the sample channel 10.

[0026] The material of each piezoelectric transducer in the ultrasonic transducer array 20 is one of the following: lead zirconate titanate (PZT4), PZT5, quartz crystal, and lithium niobate crystal. Preferably, the material of the piezoelectric transducer used for the second focusing subset is PZT5.

[0027] The particle focusing module 30 has a circular or regular polygonal cross-section with an even number of sides. When the particle focusing module 30 has a regular polygonal cross-section, the total number of piezoelectric transducers is also even and can be the same as or different from the number of sides of the particle focusing module 30. The maximum width of the sample channel 10 of the particle focusing module 30 is 100µm-1000µm. Preferably, the sample channel 10 has a circular cross-section with a maximum width of 300µm.

[0028] The particle focusing module 30 can be a capillary or a microfluidic chip. The capillary can be used in flow cytometers. The particle focusing module 30 is manufactured by integral molding or machining of one of the following materials: stainless steel, aluminum alloy, glass, quartz, plastic, resin, or polydimethylsiloxane (PDMS). Preferably, when the particle focusing module 30 uses a capillary, the material is 316L stainless steel.

[0029] The particle materials include polystyrene, magnetic beads, and cells. The particle size ranges from 1 to 50 μm, and the particle flow rate in sample channel 10 is 10 μL / min to 5000 μL / min.

[0030] The particle focusing method of the multifunctional array ultrasound particle focusing module of the present invention is as follows: The ultrasonic transducer array 20 is controlled to generate a stable sound field in the sample channel 10 for particle focusing. The particle focusing module 30 is used for particle flow. Specifically, after the particle fluid is introduced into the sample channel 10 of the particle focusing module 30, a first driving signal is output to each piezoelectric transducer in the first focusing subset of the ultrasonic transducer array 20. The first focusing subset is in driving mode to generate a sound field in the sample channel 10 for manipulating the particle fluid and performing a scanning process. The scanning of the first driving signal is a range scan, and the scanning frequency of the range scan is a preset center resonant frequency f. c The scanning frequency is ±500kHz, supplemented by 1-10kHz, with a scanning step size of 1 kHz-10 kHz, preferably 1kHz; the preset center resonant frequency f c The frequency is defined as the half-wavelength of the sound wave flowing through the sample channel 10. Then, feedback signals are received from each piezoelectric transducer in the second focusing subset for monitoring. The second focusing subset is in monitoring mode, and the feedback signals are related to the acoustic characteristic parameters of the sample channel 10, characterizing the current optimal resonant frequency of the sample channel 10. Based on the feedback signals, the optimal resonant frequency is determined. After receiving the feedback signals, each piezoelectric transducer in the second focusing subset determines the sound pressure amplitude in the sample channel 10. When the first driving signal scans, the maximum sound pressure amplitude is determined, and the frequency at which the sound pressure amplitude is at its maximum is taken as the optimal resonant frequency f. opt The frequency of the first driving signal is dynamically adjusted using a feedback control algorithm to match or track the optimal resonant frequency. Then, the ultrasonic transducer array 20 switches to focused driving mode, outputting and locking a second driving signal to each piezoelectric transducer in both the first and second focused subsets. The frequency of the second driving signal is the optimal resonant frequency f. opt Linear scanning drive is performed within the range of ±1-50kHz, not at a single frequency. The driving time for a single frequency is 1-100ms, preferably 1ms for each frequency point. Both the first and second focusing subsets are in driving mode. The phase adjustment range of the first and second driving signals is -180° to 180°, and the amplitude adjustment range is 0-30V. Then, the scanning-monitoring-locking process is repeated periodically for 1-30 minutes to achieve ultrasonic-driven particle focusing.

[0031] The present invention specifically adopts the following implementation steps: Step 1: Upon startup, the system first calculates a theoretical center resonant frequency f based on the geometry (e.g., diameter or width) of sample channel 10 and the velocity of sound in the fluid medium (typically water or buffer). c(If the cross-section is circular, the maximum width is the diameter). Then, the system uses this center frequency as a reference to set a frequency scanning range and scanning step size.

[0032] Step 2: The system drives the first focusing subset to rapidly scan the frequency of its emitted signal within a preset scanning range. Simultaneously, the second focusing subset receives the sound waves in real time and converts them into a voltage signal. The system monitors the key acoustic characteristic parameters of the feedback voltage signal, preferably the sound pressure amplitude. When the driving frequency matches the current optimal resonant frequency of the channel, resonance occurs within sample channel 10, the sound pressure amplitude reaches its maximum, and the voltage signal of the second focusing subset is at its maximum.

[0033] Step 3: The system uses a peak-finding algorithm to find the frequency point that maximizes the sound pressure amplitude and determines it as the current optimal resonant frequency f. opt Subsequently, the system switched to focused drive mode. In focused drive mode, all transducer units (including the second focused subset originally used for monitoring) were configured to drive to maximize the sound field energy. The frequency of the drive signal was set to f. opt To cope with small, rapid frequency fluctuations and further enhance stability, in f opt A linear scan drive is performed in a small area nearby.

[0034] Throughout the module's operation, the control system periodically repeats the aforementioned "scan-monitor-lock" process, thereby achieving control over f. opt Dynamic tracking and compensation ensure that the system always operates at its most efficient state.

[0035] Example 1: In a specific implementation of this invention, the particle focusing module 30 uses a 316L stainless steel capillary tube to prepare particles such as... Figure 2 The diagram shows a four-array particle focusing system. All piezoelectric transducers are 6mm wide and 50mm wide, made of PZT5 material. The spacing between each piezoelectric transducer unit along the length of the particle focusing module 30 is 0.5mm. The capillary inner diameter is 300µm, the outer diameter is 600µm, and other parameters are optimized. The preset center resonant frequency f is... c The frequency was 2.54 MHz. Frequency scanning was performed on the first focusing subset of the ultrasonic transducer array 20 within the frequency range of 2.52 MHz to 2.56 MHz, while the second focusing subset was controlled to receive the sound pressure amplitude signal. 3 μm polystyrene microparticles were focused, with a particle flow rate of 600 μL / min in the sample channel 10.

[0036] like Figure 4The image shows the focusing simulation effect when particles pass through a four-array particle focusing system. On section 10 of the sample flow channel, the particles initially exhibit linear focusing, then focus into a point shape. (See image for details.) Figure 5 As shown, this is the voltage signal converted from the sound pressure signal received by the second focusing subset, with the voltage signal being the largest at 2.55MHz. The particle focusing effect within the capillary is observed using a high-speed camera, as shown... Figure 6 The image shows the particle focusing effect within the scanning frequency range. At 2.54MHz, the focusing width of the particles within the capillary is the smallest, therefore the optimal resonant frequency f is... opt It is 2.54MHz.

[0037] Example 2: Similar to Example 1, the outer diameter of the capillary was changed to 700 μm, while the inner diameter remained at 300 μm. The preset center resonant frequency f... c The optimal resonant frequency is 2.54MHz. opt The optimal resonant frequency was determined to be approximately 2.8 MHz. In this embodiment, after determining the optimal resonant frequency to be around 2.8 MHz, all piezoelectric transducers were driven using single-frequency drive and linear sweep frequency drive within a frequency range of 2.798-2.812 MHz. 3 μm polystyrene microparticles were focused, with a particle flow rate of 800 μL / min in sample channel 10.

[0038] like Figure 7 The image shows the focusing effect corresponding to single-frequency drive and sweep-frequency drive. It can be seen that sweep-frequency drive can average the driving effect of frequencies within the sweep range, although the effect is not as good as the resonant frequency f. opt However, it can better cope with frequency drift during long-term operation.

[0039] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of this application, and not to limit them. Although this application has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of this application.

Claims

1. A multifunctional array ultrasonic-driven particle focusing system, characterized in that: The system includes an ultrasonic transducer array (20) and a particle focusing module (30) with a sample channel (10). The ultrasonic transducer array (20) includes at least two piezoelectric transducer units. The piezoelectric transducer units are arranged sequentially on the outer wall of the particle focusing module (30) along the length direction of the particle focusing module (30). Each piezoelectric transducer unit includes a pair of independently controlled piezoelectric transducers. Each pair of piezoelectric transducers is arranged opposite each other on the outer wall of the particle focusing module (30). Each pair of piezoelectric transducers is divided and constructed into a first focusing subset and a second focusing subset. The first focusing subset and the second focusing subset are driven and controlled at different times in a time-multiplexed manner, so that the first focusing subset and the second focusing subset work in the driving mode and the monitoring mode, so as to control the particle focusing system to switch the focusing driving mode and periodically repeat the scanning-monitoring-locking process, thereby realizing ultrasonic-driven particle focusing of the particle fluid in the sample channel (10).

2. The multifunctional array ultrasonic-driven particle focusing system according to claim 1, characterized in that: The piezoelectric transducers in the ultrasonic transducer array (20) are evenly spaced along the circumference of the particle focusing module (30), and the two piezoelectric transducers of each piezoelectric transducer unit are symmetrically distributed with respect to the axis of the particle focusing module (30); the piezoelectric transducer units are staggered along the length of the particle focusing module (30).

3. The particle focusing method of the multifunctional array ultrasonic particle focusing module according to any one of claims 1-2, characterized in that, include: After the particle fluid is introduced into the sample channel (10) of the particle focusing module (30), a first driving signal is output to each piezoelectric transducer in the first focusing subset of the ultrasonic transducer array (20). The first focusing subset is in driving mode to generate an acoustic field in the sample channel (10) for manipulating the particle fluid and perform a scanning process. Then, feedback signals are received from each piezoelectric transducer in the second focusing subset for monitoring. The second focusing subset is in monitoring mode. Based on the feedback signal, the optimal resonant frequency is determined, and the frequency of the first driving signal is dynamically adjusted to match the frequency of the first driving signal with the optimal resonant frequency. Then, the ultrasonic transducer array (20) switches to the focusing driving mode, outputs a second driving signal to each piezoelectric transducer in both the first and second focusing subsets and locks them. Both the first and second focusing subsets are in driving mode. Then, the scanning-monitoring-locking process is repeated periodically to realize ultrasonic-driven particle focusing.

4. The particle focusing method of the multifunctional array ultrasound particle focusing module according to claim 3, characterized in that: The scanning of the first driving signal is a range scan, and the scanning frequency of the range scan is the preset center resonant frequency f. c The scan frequency is ±500kHz, supplemented by 1-10kHz.

5. The particle focusing method of the multifunctional array ultrasound particle focusing module according to claim 3, characterized in that: After receiving the feedback signal, each piezoelectric transducer in the second focusing subset determines the sound pressure amplitude in the sample channel (10). When the first driving signal is scanned, the maximum value of the sound pressure amplitude is determined, and the frequency at which the sound pressure amplitude is at its maximum is taken as the optimal resonant frequency f. opt .

6. The particle focusing method of the multifunctional array ultrasound particle focusing module according to claim 3, characterized in that: The frequency of the second driving signal is the optimal resonant frequency f. opt Linear scan drive within the range of ±1-50kHz, with a drive time of 1-100ms for a single frequency.

7. The particle focusing method of the multifunctional array ultrasound particle focusing module according to claim 3, characterized in that: The phase adjustment range of the first driving signal and the second driving signal is -180° to 180°, and the amplitude adjustment range is 0 to 30V.

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