Cutter adjusting method, control device and system of ultra-thin section imaging device

Abstract

The invention relates to the technical field of biological section imaging, and provides a cutter adjusting method, a control device and a system for an ultrathin section imaging device, and the method comprises the following steps: pre-determining a corresponding maximum cutting influence threshold value when the imaging quality does not meet the requirement; setting a correction threshold based on the maximum cutting influence threshold; and when it is determined that the actual cumulative cutting influence reaches the correction threshold value, the angle of the cutter is corrected so as to offset angle cumulative deviation generated by the cutter within the correction threshold value. Through the arrangement, the angle correction node of the cutter can be prepositioned, the problems of imaging defocus, image blurring and the like caused by the fact that accumulated offset approaches or breaks through a critical threshold value are avoided, it is ensured that the whole-process imaging quality meets the requirement, and the problem of accumulation and superposition of micro displacement of a pitch angle and a rolling angle caused by periodic cutting force can be effectively solved; the optimal cutting angle of the cutter is maintained to realize continuous and stable imaging.

Description

Technical Field

[0001] This invention relates to the field of biological slide imaging technology, and in particular to a blade adjustment method, control device and system for an ultrathin slide imaging device. Background Technology

[0002] Fluorescence microtomography (fMOST) is one of the core technologies for analyzing the three-dimensional structure of biological tissues. This technology adopts the principle of "slicing first and then imaging". The fixed biological sample is continuously sliced ​​into ultra-thin sections (with a thickness of up to the micrometer level) and imaged layer by layer. After a specified thickness is removed from each slice, a flat section is formed on the sample surface. Then, the sample platform is raised to the corresponding thickness distance, and the flat section is completely scanned and imaged by a fluorescence microscope. This process is repeated until complete information of each layer of the biological tissue is obtained. Then, the three-dimensional spatial structure of the biological sample is reconstructed by software algorithms.

[0003] In the ultrathin sectioning process of fMOST technology, high-sharpness cutting tools are a prerequisite for obtaining precise biological sections at the nanometer to micrometer level. The radius of the cutting edge of such tools is only tens to hundreds of nanometers, which can cut a mirror-level flat section in one go, significantly reducing the operational difficulty of subsequent optical analysis, mass spectrometry detection, sequencing and pathological research.

[0004] In actual research, it was found that the quality of ultrathin sections of samples is mainly affected by two key angular parameters: the tool pitch angle and the roll angle (e.g., ...). Figures 1 to 3 (As shown). Since the sample surface area is usually larger than the blade width, an ultrathin section with a complete cross-section needs to be completed step by step through multiple sections. If the roll angle is too large, adjacent steps will form on the section surface, affecting the focusing accuracy of subsequent imaging. The pitch angle will affect the cutting smoothness of the sample surface and the tool life.

[0005] Further research revealed that traditional cutting tools, locked by friction after side mounting, inevitably experience slight changes in their pitch and roll angles under the influence of periodic cutting forces. This leads to variations in the cutting quality of the sample surface, particularly the slice thickness. Changes in the pitch angle cause the tool to be out of optimal position during slicing, resulting in poor slice quality. Changes in slice thickness cause inconsistencies between the slice thickness and the height the platform rises. Over time, these discrepancies cause the distance deviation between the sample surface and the objective lens to exceed the objective lens's depth of field (approximately 0.3 micrometers for a commonly used 40x water scope and approximately 0.13 micrometers for a commonly used 60x water scope), ultimately resulting in defocused imaging and blurred images.

[0006] In view of the above problems and findings, how to maintain the ideal working angle of the cutting tool and achieve continuous and stable imaging has become an important technical problem that urgently needs to be solved.

[0007] It should be clarified here that the above description is intended to facilitate understanding of the overall background of the present invention, and should not be construed as an admission or implication in any way that the information constitutes prior art known to those skilled in the art. Summary of the Invention

[0008] This invention provides a tool adjustment method, control device, and system for an ultrathin slice imaging device, which solves the problem that existing ultrathin slice imaging devices cannot achieve continuous and stable imaging due to tool angle deviation. It enables dynamic adjustment of the tool angle, thereby keeping the tool at the ideal working angle and achieving continuous and stable imaging.

[0009] This invention provides a blade adjustment method for an ultrathin slice imaging device, comprising the following steps: Predetermine the maximum cutting impact threshold corresponding to when the imaging quality does not meet the requirements; A correction threshold is set based on the maximum cutting impact threshold; When the actual cumulative cutting effect reaches the correction threshold, the tool angle is corrected to offset the cumulative angular offset of the tool within the correction threshold.

[0010] According to the tool adjustment method of an ultrathin slice imaging device provided by the present invention, the maximum cutting influence threshold includes the maximum cutting time, and the correction threshold is set as a preset time interval within the maximum cutting time.

[0011] According to the tool adjustment method of an ultrathin slice imaging device provided by the present invention, the maximum cutting influence threshold includes the maximum cutting stroke, and the correction threshold is set as a preset cutting stroke interval within the maximum cutting stroke.

[0012] According to the tool adjustment method of an ultrathin slice imaging device provided by the present invention, the determination of the maximum cutting influence threshold includes the following steps: The image sharpness of slices under different cumulative cutting influence thresholds is evaluated based on the image sharpness evaluation algorithm; When it is determined that the imaging quality does not meet the requirements, the cumulative cutting influence threshold at this time is obtained as the maximum cutting influence threshold.

[0013] According to the present invention, a method for adjusting the blade of an ultrathin slice imaging device includes the following steps: When the actual cumulative cutting reaches the correction threshold, the slice is defocused. The angle of the cutting tool is adjusted based on the defocusing judgment result.

[0014] According to the present invention, a blade adjustment method for an ultrathin slice imaging device includes the following steps in determining defocus: The image is sliced ​​and imaged at the current reference angle of the cutting tool, and the image sharpness index is obtained. After adjusting the cutting tool to a preset angle in the positive direction, slice the image and obtain the image sharpness index; After adjusting the cutting tool to a preset angle in the negative direction, slice the image and obtain the image sharpness index; By comparing the sharpness change trends of the three images, the defocus direction is determined based on the sharpness change trends.

[0015] According to the present invention, a method for adjusting the blade of an ultrathin slice imaging device, based on the defocus judgment result, corrects the angle of the blade, including the following steps: Based on the defocusing direction, the tool is continuously angled, and the continuous angle adjustment includes step adjustment with preset step size or dynamic step size. During the angle adjustment process, slicing operations are performed simultaneously, and the image sharpness index of the slices is detected in real time to generate a continuous dataset of angle and sharpness indexes. The sharpness index is analyzed in real time, and the maximum sharpness value and the corresponding target angle are determined based on three non-monotonic sharpness indices. Adjust the cutting tool to the target angle.

[0016] The present invention also provides a tool adjustment control device, comprising: The determination module is used to pre-determine the maximum cutting impact threshold when the imaging quality does not meet the requirements; The setting module is used to set a correction threshold based on the maximum cutting influence threshold; The correction module is used to correct the tool angle when the actual cumulative cutting reaches the correction threshold, so as to offset the cumulative angular offset of the tool within the correction threshold.

[0017] According to a tool adjustment control device provided by the present invention, the correction module includes: The defocus determination unit is configured to slice and image at the current reference angle of the tool and obtain the image sharpness index; adjust the tool to a preset angle in the positive direction and slice and image to obtain the image sharpness index; adjust the tool to a preset angle in the negative direction and slice and image to obtain the image sharpness index; compare the sharpness change trend of the three images and determine the defocus direction based on the sharpness change trend. An angle correction unit is configured to continuously adjust the angle of the tool based on the defocus direction. The continuous angle adjustment includes a step adjustment with a preset step size or a dynamic step size. During the angle adjustment, a slicing operation is performed simultaneously, and the image sharpness index of the slice is detected in real time to generate a continuous angle and sharpness index dataset. The sharpness index is analyzed in real time, and the maximum sharpness value and the corresponding target angle are determined based on three non-monotonic sharpness indices. The tool is then adjusted to the target angle.

[0018] The present invention also provides an ultrathin slice imaging system, comprising: a three-dimensional platform, a probe structure, a cutting tool, and a roll angle adjustment device and a pitch angle adjustment device connected to the cutting tool; It also includes the tool adjustment control device described in any one of the above, wherein the tool adjustment control device is communicatively connected to the roll angle adjustment device and the pitch angle adjustment device, and is used to control the roll angle adjustment device and the pitch angle adjustment device to adjust the roll angle and pitch angle of the tool.

[0019] The present invention also provides an electronic device, including a memory, a processor, and a computer program stored in the memory and executable on the processor, wherein the processor executes the computer program to implement the blade adjustment method of any of the above-described ultrathin slice imaging devices.

[0020] The tool adjustment method, control device, and system of the ultrathin section imaging device provided by this invention, through a closed-loop control method of "threshold preset - correction trigger - angle calibration", first pre-calibrates the maximum cutting influence threshold for substandard imaging quality, and clarifies the "safety boundary" in the cutting process. Then, based on the maximum threshold, a correction threshold is set. When the actual cumulative cutting influence reaches the correction threshold, the tool angle is corrected in time to offset the cumulative angular offset generated by the tool within the threshold range. This not only advances the tool angle correction node, avoiding problems such as image defocusing and image blurring caused by cumulative offset approaching or exceeding the critical threshold, ensuring that the imaging quality meets the requirements throughout the process, but also effectively solves the problem of cumulative superposition of small displacements of pitch angle and roll angle caused by periodic cutting force, maintaining the consistency between the optimal cutting position of the tool and the slice thickness, and the cutting smoothness of the sample surface. At the same time, by continuously suppressing the cumulative effect of angular offset, it avoids the superposition and amplification of slice thickness fluctuations and platform rise height deviations, providing high-quality raw data for the three-dimensional reconstruction of biological tissue structures and improving the accuracy and reliability of three-dimensional reconstruction. Attached Figure Description

[0021] To more clearly illustrate the technical solutions in this invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of this invention. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0022] Figure 1 This is a schematic diagram of the tool roll angle and pitch angle.

[0023] Figure 2 This is a schematic diagram illustrating the effect of the tool roll angle.

[0024] Figure 3 This is a schematic diagram illustrating the effect of the tool pitch angle.

[0025] Figure 4 This is a schematic diagram of the structure of the ultrathin slice imaging system provided in an embodiment of the present invention.

[0026] Figure 5 This is a schematic flowchart of the tool adjustment method provided in an embodiment of the present invention.

[0027] Figure 6 This is a schematic diagram of the process for determining the maximum cutting influence threshold provided in an embodiment of the present invention.

[0028] Figure 7 This is a schematic diagram of the tool angle correction process provided in an embodiment of the present invention.

[0029] Figure 8 This is a schematic diagram of the defocus judgment process provided in an embodiment of the present invention.

[0030] Figure 9 This is a schematic diagram of the process for correcting the tool angle based on the defocusing result provided in an embodiment of the present invention.

[0031] Figure 10 This is a schematic diagram of the tool adjustment and control device provided in an embodiment of the present invention.

[0032] Figure 11 This is a schematic diagram of the structure of the electronic device provided in an embodiment of the present invention.

[0033] Figure label: 11. Detection structure; 111. Objective lens; 12. Conventional tool holder fixing plate; 13. Tool; 14. Sample; 15. 3D platform; 16. Roll angle adjustment device; 17. Pitch angle adjustment device; 21. Determination module; 22. Setting module; 23. Correction module; 231. Defocus judgment unit; 232. Angle correction unit; 31. Processor; 32. Communication interface; 33. Memory; 34. Communication bus. Detailed Implementation

[0034] To make the objectives, technical solutions, and advantages of this invention clearer, the technical solutions of this invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of this invention. All other embodiments obtained by those skilled in the art based on the embodiments of this invention without creative effort are within the scope of protection of this invention.

[0035] To better understand the blade adjustment method, control device, and system of the ultrathin slice imaging device provided in the embodiments of the present invention, its application background is first introduced. In the ultrathin slice stage of fMOST technology, a high-sharpness blade is a prerequisite for obtaining nano- to micron-level precision biological slices, and the quality of the ultrathin slice of the sample is mainly affected by two key angle parameters: the blade pitch angle and the roll angle.

[0036] Traditional cutting tools are locked in place by friction after being mounted on the side. Under the influence of periodic cutting forces, the pitch and roll angles of the tool inevitably undergo slight changes, leading to variations in the cutting quality of the sample surface, especially the slice thickness. Changes in the pitch angle cause the tool to be out of optimal position during slicing, resulting in poor slice quality. Changes in slice thickness cause the slice thickness to be inconsistent with the height of the platform rise. Over time, this discrepancy causes the distance deviation between the sample surface and the objective lens to exceed the depth of field of the objective lens (the depth of field of a commonly used 40x water scope is about 0.3 micrometers, and that of a commonly used 60x water scope is about 0.13 micrometers), ultimately resulting in a blurred image due to defocusing.

[0037] In view of the above problems, embodiments of the present invention provide a tool adjustment method, control device and system for an ultrathin slice imaging device, which can realize dynamic adjustment of the tool angle, thereby keeping the tool at an ideal working angle and achieving continuous and stable imaging.

[0038] The following is combined with Figures 1 to 11 The present invention describes the blade adjustment method, control device, and system of the ultrathin slice imaging apparatus.

[0039] Reference Figure 4 and Figure 5 A method for adjusting the cutting tool in an ultrathin slice imaging device includes the following steps: Step S1: Predetermine the maximum cutting impact threshold corresponding to the situation where the imaging quality does not meet the requirements.

[0040] Step S2: Set a correction threshold based on the maximum cutting impact threshold.

[0041] Step S3: When the actual cumulative cutting effect reaches the correction threshold, the angle of the tool 13 is corrected to offset the cumulative angular offset of the tool 13 within the correction threshold.

[0042] This setup, through a closed-loop control method of "threshold preset - correction trigger - angle calibration," first pre-calibrates the maximum cutting impact threshold that results in substandard imaging quality, clarifying the "safety boundary" during the cutting process. Then, based on this maximum threshold, a correction threshold is set. When the actual cumulative cutting impact reaches the correction threshold, the angle of the tool 13 is corrected in a timely manner to offset the cumulative angle offset generated by the tool 13 within the threshold range. This not only advances the angle correction node of the tool 13, avoiding problems such as image defocusing and image blurring caused by cumulative offset approaching or exceeding the critical threshold, ensuring that the imaging quality meets the requirements throughout the process, but also effectively solves the problem of cumulative superposition of small displacements of pitch and roll angles caused by periodic cutting forces. It maintains the consistency between the optimal cutting position of the tool 13 and the slice thickness, as well as the cutting smoothness of the sample surface. At the same time, by continuously suppressing the cumulative effect of angle offset, it avoids the superposition and amplification of slice thickness fluctuations and platform rise height deviations, providing high-quality raw data for the reconstruction of three-dimensional structures of biological tissues and improving the accuracy and reliability of three-dimensional reconstruction.

[0043] In one example of the present invention, the maximum cutting impact threshold can be configured as the cumulative cutting time, and the correction threshold is set as a preset time interval within the cumulative cutting time.

[0044] In detail, as the cumulative cutting time increases, the tool 13 continuously bears the effect of periodic cutting force, and the number of contact friction and impact with the sample continues to accumulate. The mounting structure, which was originally locked by side friction force, will experience slight stress release and wear, which in turn leads to the gradual accumulation of angular offset and the increase of micro displacement change. When the cumulative cutting time reaches a certain critical value, the angular offset of the tool 13 will exceed the upper limit allowed by the imaging quality. At this time, the critical value is the maximum cutting time. By setting a time interval within the maximum cutting time to correct the angle of the tool 13, the angle correction node of the tool 13 can be moved forward, avoiding problems such as image defocusing and image blurring caused by the cumulative offset approaching or exceeding the critical value.

[0045] For example, if it is determined through experiments or experience that the imaging quality is not up to standard after 5 hours of cumulative cutting, the correction threshold can be set to time intervals of 1h / time, 2h / time, 2.5h / time, 3h / time, etc., so that after the actual cumulative cutting time reaches the time intervals of 1h, 2h, 2.5h, 3h, etc., the angle of the tool 13 can be corrected, thereby keeping the tool 13 at the ideal working angle.

[0046] In another example of the present invention, the maximum cutting influence threshold can also be configured as the cumulative cutting stroke, and the correction threshold is set to a preset cutting stroke interval within the cumulative cutting stroke.

[0047] In detail, the cutting stroke directly reflects the actual physical distance at which the cutting edge of tool 13 participates in cutting. The longer the stroke, the more obvious the cumulative effect of frictional wear and periodic impact between the cutting edge of tool 13 and the sample. The angular offset will also accumulate synchronously with the increase of the stroke. When the accumulated cutting stroke reaches a certain critical value, the angular offset of tool 13 will exceed the upper limit allowed by the imaging quality. At this time, the critical value is the maximum cutting stroke. By presetting the cutting stroke interval within the maximum cutting stroke to correct the angle of tool 13, the angle correction node of tool 13 can be moved forward, avoiding problems such as image defocusing and image blurring caused by the accumulated offset approaching or exceeding the critical value.

[0048] For example, if it is determined through experiments or experience that the imaging quality fails when the maximum cutting stroke is 50mm, the correction threshold can be set to 10mm / time, 15mm / time, 20mm / time, 30mm / time, etc., so that after the actual cutting stroke reaches the cutting stroke interval of 10mm, 15mm, 20mm, 30mm, etc., the angle of the tool 13 is corrected, thereby keeping the tool 13 at the ideal working angle.

[0049] In one example of the present invention, reference is made to Figure 6 The determination of the maximum cumulative cutting impact threshold includes the following steps: Step S11: Evaluate the image sharpness of slices under different cumulative cutting influence thresholds based on the image sharpness evaluation algorithm.

[0050] Step S12: When the imaging quality does not meet the requirements, obtain the cumulative cutting influence threshold at this time as the maximum cutting influence threshold.

[0051] In detail, in individual experimental scenarios or during routine slice imaging with the fMOST device, images can be automatically saved when a certain cumulative cutting impact threshold is reached. For example, when the cumulative cutting time reaches 1h, 1.5h, 2h, 3h, etc., or the cumulative cutting stroke reaches 10mm, 15mm, 20mm, 30mm, etc., the scanned image of that layer is saved. The saved images are then imported into conventional algorithm analysis software, and image sharpness evaluation algorithms, such as the Laplacian variance method, are used to calculate the image sharpness value at each threshold node. Combining the depth of field and 3D reconstruction requirements of the objective lens, a sharpness qualification threshold is set. By comparing the sharpness qualification threshold with the image sharpness values ​​at each threshold node, the cumulative cutting impact threshold corresponding to the first time the sharpness falls below the qualification threshold is selected as the maximum cutting impact threshold.

[0052] In one example of the present invention, reference is made to Figure 7 After determining the maximum cutting influence threshold and the correction threshold, the angle of tool 13 is corrected whenever the actual cumulative cutting influence reaches the correction threshold, specifically including the following steps: Step S31: When the actual cumulative cutting reaches the correction threshold, perform a defocus judgment on the slice.

[0053] To elaborate further, refer to Figure 8 Defocus detection includes the following steps: Step S311: Slice and image the image at the current reference angle of the tool 13 and obtain the image sharpness index.

[0054] Step S312: After adjusting the cutter 13 to a preset angle in the positive direction, slice and image the image and obtain the image clarity index.

[0055] Step S313: After adjusting the cutter 13 to a preset angle along the negative direction, slice and image the image and obtain the image clarity index.

[0056] Step S314: Compare the sharpness change trends of the three images, and determine the defocus direction based on the sharpness change trends.

[0057] In detail, when the actual cumulative cutting effect reaches the correction threshold, the tool 13 maintains its current reference angle, performs image scanning on the slice surface after slicing, and evaluates the image sharpness index VL0 using image sharpness evaluation algorithms such as the Laplacian variance method. Then, based on the reference angle, the tool 13 is adjusted in the positive direction to a preset angle for slicing and imaging, and the image sharpness index VL+ at that angle is evaluated. Then, the tool 13 is returned to the reference angle, and based on the reference angle, the tool 13 is adjusted in the negative direction to a preset angle for slicing and imaging, and the image sharpness index VL- at that angle is evaluated. By comparing the sharpness change trends of the three imaging operations, the angle adjustment direction of the tool 13 can be determined. For example, if VL- < VL0 < VL+, it indicates that the angle of the tool 13 is shifted in the negative direction, and the angle of the tool 13 needs to be corrected in the positive direction to offset the shift. Conversely, if VL+ < VL0 < VL-, it indicates that the angle of the tool 13 is shifted in the positive direction, and the angle of the tool 13 needs to be corrected in the negative direction to offset the shift.

[0058] It should be noted here that the defocus direction determination of the pitch angle and roll angle of tool 13 needs to be performed separately and determined independently.

[0059] Step S32: Adjust the angle of the tool 13 based on the defocus judgment result.

[0060] To elaborate further, refer to Figure 9 Step S32 includes: S321. Based on the defocusing direction, the tool 13 is continuously angled, including step adjustment with preset step size or dynamic step size. S322. During the angle adjustment process, the slicing operation is performed simultaneously, and the image sharpness index of the slice is detected in real time to generate a continuous dataset of angle and sharpness index. S323. Perform real-time analysis of the sharpness index, and determine the maximum sharpness value and the corresponding target angle based on three non-monotonic sharpness indices. S324. Adjust tool 13 to the target angle.

[0061] Based on the aforementioned determination of the pitch angle and roll angle of the tool 13, the tool 13 is continuously adjusted in angle. The adjustment method can be a step-by-step adjustment with a preset step size or a dynamic step size. The preset step size can be set to 0.0005 degrees / step, 0.001 degrees / step, 0.002 degrees / step, etc. The dynamic step size can be adaptively adjusted according to the real-time sharpness change rate. For example, when the sharpness improves rapidly, the step size is kept at 0.001 degrees, and when the improvement slows down, the step size is reduced to 0.0005 degrees. During the continuous angle adjustment, the ultrathin slicing operation is performed simultaneously. The imaging sharpness index of each slice is evaluated by the image sharpness evaluation algorithm to form a continuous dataset of angle-sharpness index.

[0062] The sharpness indicators acquired in real time can be dynamically analyzed using algorithms such as second-order difference. When three sharpness indicators that are not monotonically changing are detected, i.e., a "rise-peak-fall" trend is observed, and the three indicators are not monotonically increasing or decreasing, the maximum sharpness value in this trend and the corresponding angle are determined as the target angle. Then, the angle of the tool 13 is adjusted to the target angle to complete the angle correction and ensure that the tool 13 is restored to the optimal cutting position, thus ensuring the consistency of subsequent slice thickness and the sharpness of the image.

[0063] The tool adjustment control device provided by the present invention is described below. The tool adjustment control device described below can be referred to in correspondence with the tool adjustment method of the ultrathin slice imaging device described above.

[0064] Reference Figure 10 A tool adjustment control device includes a determining module 21, a setting module 22, and a correction module 23. The determining module 21 is used to predetermine the maximum cutting influence threshold corresponding to when the imaging quality does not meet the requirements. The setting module 22 is used to set a correction threshold according to the maximum cutting influence threshold. The correction module 23 is used to correct the angle of the tool 13 when the actual cumulative cutting reaches the correction threshold, so as to offset the cumulative angle offset of the tool 13 within the correction threshold.

[0065] In detail, the correction module 23 includes a defocus judgment unit 231 and an angle correction unit 232. The defocus judgment unit 231 is configured to: slice and image at the current reference angle of the cutter 13 and obtain the image sharpness index; adjust the cutter 13 to a preset angle in the positive direction and slice and image again, adjust the cutter 13 to a preset angle in the negative direction and slice and image again, compare the sharpness change trends of the three images, and determine the defocus direction based on the sharpness change trend. The angle correction unit 232 is configured to: continuously adjust the angle of the cutter 13 based on the defocus direction, including step-by-step adjustment with preset or dynamic step sizes; simultaneously perform slicing operations during angle adjustment and detect the image sharpness index of the slice in real time; generate a continuous angle and sharpness index dataset; perform real-time analysis of the sharpness index; determine the maximum sharpness value and the corresponding target angle based on three non-monotonic sharpness indices; and adjust the cutter 13 to the target angle.

[0066] On the other hand, the present invention also provides a schematic diagram of the physical structure of an electronic device, such as... Figure 11 As shown, the electronic device may include a processor 31, a communication interface 32, a memory 33, and a communication bus 34. The processor 31, communication interface 32, and memory 33 communicate with each other via the communication bus 34. The processor 31 can call logical instructions in the memory 33 to execute a tool adjustment method for the ultrathin slice imaging device. This method includes the following steps: pre-determining the maximum cutting influence threshold corresponding to when the imaging quality does not meet the requirements; setting a correction threshold based on the maximum cutting influence threshold; and correcting the angle of the tool 13 when the actual cumulative cutting influence reaches the correction threshold to offset the cumulative angular offset of the tool 13 within the correction threshold.

[0067] Furthermore, the logical instructions in the aforementioned memory 33 can be implemented as software functional units and, when sold or used as independent products, can be stored in a computer-readable storage medium. Based on this understanding, the technical solution of the present invention, or the part that contributes to the prior art, or a part of the technical solution, can be embodied in the form of a software product. This computer software product is stored in a storage medium and includes several instructions to cause a computer device (which may be a personal computer, server, or network device, etc.) to execute all or part of the steps of the methods described in the various embodiments of the present invention. The aforementioned storage medium includes various media capable of storing program code, such as USB flash drives, portable hard drives, read-only memory (ROM), random access memory (RAM), magnetic disks, or optical disks.

[0068] On the other hand, the present invention also provides an ultrathin slice imaging system, including a three-dimensional platform 15, a probe structure 11, a cutting tool 13, and a roll angle adjustment device 16 and a pitch angle adjustment device 17 connected to the cutting tool 13; it also includes a cutting tool adjustment control device provided in any of the above examples, the cutting tool adjustment control device being communicatively connected to the roll angle adjustment device 16 and the pitch angle adjustment device 17, for controlling the roll angle adjustment device 16 and the pitch angle adjustment device 17 to adjust the roll angle and pitch angle of the cutting tool 13 when the actual cumulative cutting effect reaches a correction threshold.

[0069] It should be noted that the specific structures of the three-dimensional platform 15, the detection structure 11, and the tool 13 can refer to existing fluorescence microtomography, while the control roll angle adjustment device 16 and the pitch angle adjustment device 17 can adopt existing electric angular displacement platforms to precisely adjust the roll angle and pitch angle of the tool 13.

[0070] It is understood that, without contradiction, those skilled in the art can combine and integrate the different embodiments or examples described in this specification, as well as the features of the different embodiments or examples.

[0071] The tool adjustment method, control device, and system of the ultrathin slice imaging device provided in this invention, through the closed-loop control method of "threshold preset - correction trigger - angle calibration", can not only advance the angle correction node of the tool 13 to avoid problems such as image defocusing and image blurring caused by the cumulative offset approaching or exceeding the critical threshold, ensuring that the imaging quality meets the requirements throughout the process, but also effectively solve the problem of the cumulative superposition of small displacements of pitch angle and roll angle caused by periodic cutting force, maintain the consistency between the optimal cutting position of the tool 13 and the slice thickness, and the cutting smoothness of the sample surface, but also avoid the superposition and amplification of slice thickness fluctuation and platform rise height deviation by continuously suppressing the cumulative effect of angle offset, providing high-quality raw data for the reconstruction of three-dimensional structure of biological tissue, and improving the accuracy and reliability of three-dimensional reconstruction.

[0072] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A method for adjusting the cutting tool in an ultrathin slice imaging device, characterized in that, Includes the following steps: Predetermine the maximum cutting impact threshold corresponding to when the imaging quality does not meet the requirements; A correction threshold is set based on the maximum cutting impact threshold; When the actual cumulative cutting effect reaches the correction threshold, the angle of the tool (13) is corrected to offset the cumulative angular offset of the tool (13) within the correction threshold.

2. The blade adjustment method of the ultrathin slice imaging device according to claim 1, characterized in that, The maximum cutting impact threshold includes the maximum cutting time, and the correction threshold is set to a preset time interval within the maximum cutting time.

3. The blade adjustment method of the ultrathin slice imaging device according to claim 1, characterized in that, The maximum cutting impact threshold includes the maximum cutting stroke, and the correction threshold is set as a preset cutting stroke interval within the maximum cutting stroke.

4. The blade adjustment method of the ultrathin slice imaging device according to any one of claims 1 to 3, characterized in that, The determination of the maximum cutting influence threshold includes the following steps: The image sharpness of slices under different cumulative cutting influence thresholds is evaluated based on the image sharpness evaluation algorithm; When it is determined that the imaging quality does not meet the requirements, the cumulative cutting influence threshold at this time is obtained as the maximum cutting influence threshold.

5. The blade adjustment method of the ultrathin slice imaging device according to claim 4, characterized in that, The correction of the angle of the cutting tool (13) includes the following steps: When the actual cumulative cutting reaches the correction threshold, the slice is defocused. The angle of the cutting tool (13) is corrected based on the defocus judgment result.

6. The blade adjustment method of the ultrathin slice imaging device according to claim 5, characterized in that, The defocus determination includes the following steps: The image is sliced ​​and imaged at the current reference angle of the cutting tool (13) to obtain the image sharpness index; After adjusting the cutting tool (13) to a preset angle in the positive direction, slice the image and obtain the image clarity index; After adjusting the cutter (13) to a preset angle in the negative direction, slice the image and obtain the image clarity index; By comparing the sharpness change trends of the three images, the defocus direction is determined based on the sharpness change trends.

7. The blade adjustment method of the ultrathin slice imaging device according to claim 6, characterized in that, The angle of the tool (13) is corrected based on the defocus judgment result, including the following steps: Based on the defocusing direction, the tool (13) is continuously angled, and the continuous angle adjustment includes step adjustment with preset step size or dynamic step size; During the angle adjustment process, slicing operations are performed simultaneously, and the image sharpness index of the slices is detected in real time to generate a continuous dataset of angle and sharpness indexes. The sharpness index is analyzed in real time, and the maximum sharpness value and the corresponding target angle are determined based on three non-monotonic sharpness indices. Adjust the cutting tool (13) to the target angle.

8. A tool adjustment and control device, characterized in that, include: The determination module (21) is used to pre-determine the maximum cutting influence threshold when the imaging quality does not meet the requirements; Setting module (22) is used to set a correction threshold according to the maximum cutting influence threshold; The correction module (23) is used to correct the angle of the tool (13) when the actual cumulative cutting reaches the correction threshold, so as to offset the cumulative angle offset of the tool (13) within the correction threshold.

9. The tool adjustment control device according to claim 8, characterized in that, The correction module (23) includes: The defocus determination unit (231) is configured to slice and image at the current reference angle of the cutter (13) and obtain the image sharpness index, adjust the cutter (13) to a preset angle in the positive direction and slice and image and obtain the image sharpness index, adjust the cutter (13) to a preset angle in the negative direction and slice and image and obtain the image sharpness index, compare the sharpness change trend of the three images, and determine the defocus direction based on the sharpness change trend. Angle correction unit (232) is configured to continuously adjust the angle of the tool (13) based on the defocus direction. The continuous angle adjustment includes step adjustment with a preset step size or a dynamic step size. During the angle adjustment, slicing operation is performed simultaneously, and the image sharpness index of the slice is detected in real time to generate a continuous angle and sharpness index dataset. The sharpness index is analyzed in real time, and the maximum sharpness value and the corresponding target angle are determined based on three non-monotonic sharpness indices. The tool (13) is adjusted to the target angle.

10. An ultrathin slice imaging system, characterized in that, include: The three-dimensional platform (15), the detection structure (11), the cutting tool (13), and the roll angle adjustment device (16) and the pitch angle adjustment device (17) connected to the cutting tool (13). It also includes a tool adjustment control device as described in claim 8 or 9, wherein the tool adjustment control device is communicatively connected to the roll angle adjustment device (16) and the pitch angle adjustment device (17), and is used to control the roll angle adjustment device (16) and the pitch angle adjustment device (17) to adjust the roll angle and pitch angle of the tool (13) when the actual cumulative cutting influence reaches the correction threshold.

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