Cover glass slide angle adjusting device of laser confocal microscope and use method of cover glass slide angle adjusting device

Abstract

The invention relates to the technical field of microscopes, in particular to a cover glass slide angle adjusting device of a laser confocal microscopy and a using method of the cover glass slide angle adjusting device of the laser confocal microscopy, and the cover glass slide angle adjusting device comprises a supporting bracket, a rotating rod, a rotating disc, a placing plate, a fixing frame, a telescopic spring, a clamping frame, an indicating component and a limiting component. Therefore, an operator not only can visually scan and detect the surface of the blade, but also can scan the cross section of the blade after turning over the cover glass slide on the placing plate.

Description

Technical Field

[0001] This invention relates to the field of microscopy, and more particularly to a cover slide angle adjustment device for a laser confocal microscope and its usage method. Background Technology

[0002] Traditional equipment, by introducing a laser light source and a conjugate focusing pinhole device, combined with computer image processing technology, has broken through the diffraction limit of traditional microscopes, improving the resolution by 30%-40%, and realizing optical slicing and three-dimensional reconstruction functions.

[0003] The introduction of hybrid laser and ultraviolet laser technologies in existing laser confocal microscopy further expands its multi-wavelength excitation capabilities, making CLSM a key tool in fields such as morphology, molecular biology, and neuroscience. The core principle of laser confocal microscopy lies in using a laser beam to scan the sample point by point, allowing only the focal plane signal to pass through the pinhole detector, effectively suppressing off-focal plane interference, thereby obtaining high-contrast, high-resolution images of subcellular structures.

[0004] However, when existing coverslips and slides are used to add liquid and then a test leaf is added for testing, they can only visually scan the surface of the live leaf and cannot scan and collect signals related to the cross-section. Summary of the Invention

[0005] The purpose of this invention is to provide a cover slide angle adjustment device for a laser confocal microscope and its usage method, which solves the problem that when the existing cover slide and slide are dripped with liquid and then a detection leaf is added for detection, it can only visually scan the surface of the live leaf and cannot scan and collect cross-sectional signals.

[0006] To achieve the above objectives, the present invention provides a coverslip angle adjustment device for a laser confocal microscope, comprising a support bracket, a rotating rod, a rotating disk, a placement plate, a fixing frame, a telescopic spring, a clamping frame, an indicating component, and a limiting component. The rotating rod is rotatably connected to the support bracket and located on one side of the support bracket. The rotating disk is fixedly connected to the rotating rod and located on one side of the rotating rod. The placement plate is fixedly connected to the rotating rod and located on the side of the rotating rod away from the rotating disk. The fixing frame is fixedly connected to the placement plate and located on one side of the placement plate. The telescopic spring is fixedly connected to the fixing frame and located on one side of the fixing frame. The clamping frame is connected to the fixing frame via the limiting component and is connected to the telescopic spring, and is located on the side of the fixing frame closer to the placement stage. The indicating component is disposed on the support bracket and connected to the rotating disk. The limiting component is disposed on the fixing frame and connected to the clamping frame.

[0007] The indicating component includes a scale sticker and a pointer. The scale sticker is fixedly connected to the rotating disk and located on one side of the rotating disk. The pointer is fixedly connected to the support bracket and located on the side of the support bracket closer to the scale sticker.

[0008] The limiting component includes a limiting cylinder and a limiting rod. The limiting cylinder is fixedly connected to the fixing frame and is located on one side of the fixing frame. The limiting rod is slidably connected to the limiting cylinder and fixedly connected to the clamping frame, and is located on the side of the limiting cylinder closer to the clamping frame.

[0009] The cover slide angle adjustment device of the laser confocal microscope further includes a limiting ring, which is fixedly connected to the rotating rod and located on the side of the rotating rod near the support bracket.

[0010] The cover slide angle adjustment device of the laser confocal microscope also includes a rubber pad, which is fixedly connected to the clamping frame and located on the side of the clamping frame near the placement plate.

[0011] The cover slide angle adjustment device of the laser confocal microscope also includes a locking bolt, which is disposed on the support bracket and abuts against the rotating rod.

[0012] The cover slide angle adjustment device of the laser confocal microscope further includes a fixing component, which includes a first fixing ring and a second fixing ring. The first fixing ring is fixedly connected to the fixing frame and the telescopic spring, respectively; the second fixing ring is fixedly connected to the clamping frame and the telescopic spring, respectively.

[0013] The cover slide angle adjustment device of the laser confocal microscope further includes a moving component, which includes a moving frame and a pull ring. The moving frame is fixedly connected to the clamping frame and is located on one side of the clamping frame; the pull ring is fixedly connected to the moving frame and is located on the side of the moving frame away from the clamping frame.

[0014] A method for using a coverslip angle adjustment device for a laser confocal microscope, characterized by comprising the following steps: Fix the leaf sample in the center of the glass slide and cover it with a coverslip; Move the clamp to place the cover glass slide between the clamp and the placement stage; Release the clamping frame to hold and fix the cover glass slide on the placement stage; The laser confocal microscope body is turned on to scan the surface (back) of the leaf sample in the cover glass slide; Rotate the rotating disk to cause the placement platform to flip; The microscope body is used to scan the cross-section of the sample in the flipped coverslip.

[0015] This invention discloses a cover slide angle adjustment device and its usage method for a laser confocal microscope. After the cover slide and slide are infused with a drop of liquid, a test blade is added and placed on a placement plate for testing. When flipping is required, the operator moves the clamp upwards, placing the cover slide and slide on the placement plate. The clamp is then released, allowing it to spring back under the deformation of a telescopic spring. The clamp, in conjunction with the placement plate, fixes the cover slide on the placement plate. The operator rotates the rotating disk to drive the rotating rod, which in turn flips the placement plate. The flipped placement plate then scans the cross-section of the blade in the cover slide. Through the clamping and fixing by the clamp and the rotation of the placement plate by the rotating rod, the operator can not only visually scan the surface of the test blade but also scan the cross-section of the blade after flipping the cover slide on the placement plate. Attached Figure Description

[0016] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the accompanying drawings used in the description of the embodiments or the prior art will be briefly introduced below.

[0017] Figure 1 This is a schematic diagram of the cover slide angle adjustment device of the laser confocal microscope according to the first embodiment of the present invention.

[0018] Figure 2 This is a schematic diagram of the support bracket according to the first embodiment of the present invention.

[0019] Figure 3 This is a schematic diagram of the structure of the fixing frame according to the first embodiment of the present invention.

[0020] Figure 4 This is a schematic diagram of the cover slide angle adjustment device of the laser confocal microscope according to the second embodiment of the present invention.

[0021] Figure 5 This is a schematic diagram of the cover slide angle adjustment device of the laser confocal microscope according to the third embodiment of the present invention.

[0022] Figure 6 This is a step diagram illustrating the method of using the coverslip angle adjustment device of the laser confocal microscope according to the fourth embodiment of the present invention.

[0023] In the diagram: 101-Microscope body, 102-Support bracket, 103-Rotating rod, 104-Rotating disk, 105-Placement plate, 106-Fixed frame, 107-Telescopic spring, 108-Clamping frame, 109-Limiting ring, 110-Rubber pad, 111-Locking bolt, 112-Scale sticker, 113-Pointer, 114-Limiting cylinder, 115-Limiting rod, 201-First fixing ring, 202-Second fixing ring, 301-Moving frame, 302-Pull ring. Detailed Implementation

[0024] The embodiments of the present invention are described in detail below. Examples of the embodiments are shown in the accompanying drawings. The embodiments described below with reference to the accompanying drawings are exemplary and intended to explain the present invention, but should not be construed as limiting the present invention.

[0025] The first embodiment of this application is as follows: Please see Figures 1-3 ,in Figure 1 This is a schematic diagram of the cover slide angle adjustment device of the laser confocal microscope according to the first embodiment of the present invention. Figure 2 This is a schematic diagram of the support bracket according to the first embodiment of the present invention. Figure 3 This is a schematic diagram of the structure of the fixing frame according to the first embodiment of the present invention.

[0026] This invention provides a coverslip angle adjustment device for a laser confocal microscope, comprising a support bracket 102, a rotating rod 103, a rotating disk 104, a placement plate 105, a fixing frame 106, a telescopic spring 107, a clamping frame 108, an indicating component, a limiting component, a limiting ring 109, a rubber pad 110, and a locking bolt 111. The indicating component includes a scale sticker 112 and a pointer 113, and the limiting component includes a limiting cylinder 114 and a limiting rod 115. This solution solves the problem that existing methods, when adding a detection blade after dripping liquid onto the coverslip and slide for testing, can only visually scan the surface of the live blade and cannot scan and acquire cross-sectional signals.

[0027] In this embodiment, the support bracket 102 can be mounted on the microscope body 101. The rotating rod 103 is rotatably connected to the support bracket 102 and is located on one side of the support bracket 102. The rotating disk is fixedly connected to the rotating rod 103 and is located on one side of the rotating rod 103. The placement plate 105 is fixedly connected to the rotating rod 103 and is located on the side of the rotating rod 103 away from the rotating disk 104. The fixing frame 106 is fixedly connected to the placement plate 105 and is located on one side of the placement plate 105. The telescopic spring 107 is fixedly connected to the fixing frame 106 and is located on one side of the fixing frame 106. The clamping frame 108 is connected to the fixing frame 106 via... The limiting component is connected to and connected to the telescopic spring 107, and is located on the side of the fixing frame 106 near the placement stage. The indicating component is disposed on the support bracket 102 and connected to the rotating disk 104. The limiting component is disposed on the fixing frame 106 and connected to the clamping frame 108. The microscope body 101 is a laser confocal microscope. The laser confocal microscope is based on optical conjugate focusing, which scans the sample point by point with a laser and receives the focal signal. Combined with longitudinal scanning, a high-resolution three-dimensional image is reconstructed. The laser confocal microscope is existing technology and will not be described in detail here. There are two support brackets 102, and the two support brackets 102 are welded to the lower part of the lens of the microscope body 101. There are two rotating rods 103, which are rotatably connected to the corresponding two support brackets 102. There are also two rotating disks 104, which are welded to the corresponding two rotating rods 103. The rotating disks 104 allow the operator to easily rotate the rotating rods 103. A placement plate 105 is welded to the two rotating rods 103. Two fixing brackets 106 are welded to the placement plate 105. Multiple telescopic springs 107 are welded to the two fixing brackets 106. Two clamping brackets 108 are welded to the multiple telescopic springs 107. The cover glass and slide are placed on the placement plate 105, and... The deformation capability of the telescopic spring 107 allows the clamping frame 108 to abut and clamp the cover glass slide on the placement plate 105. The indicating member is mounted on the support bracket 102 and connected to the rotating disk 104, providing accurate indication of the rotation angle of the rotating disk 104. The limiting member is mounted on the fixing frame 106 and connected to the clamping frame 108, providing connection support and directional limitation for the movement of the clamping frame 108. This allows for the addition of a detection blade after the cover glass slide and slide have been dripped with liquid and placed on the placement plate 105 for testing. When it needs to be flipped, the operator moves the clamping frame 108 upwards.The cover glass and slide are placed on the placement plate 105. The clamping frame 108 is released, allowing it to spring back to its original position under the deformation of the telescopic spring 107. This, in conjunction with the placement plate 105, fixes the cover glass on the placement table. The operator rotates the rotating disk 104 to rotate the rotating rod 103, causing the placement table to flip. This flipped placement plate 105 allows for scanning of the cross-section of the blades on the cover glass. The clamping and fixing by the clamping frame 108 and the flipping of the placement plate 105 by the rotating rod 103 enable the operator to not only visually scan the surface of the blades but also to scan the cross-section of the blades after flipping the cover glass on the placement plate 105.

[0028] The scale sticker 112 is fixedly connected to the rotating disk 104 and located on one side of the rotating disk 104; the pointer 113 is fixedly connected to the support bracket 102 and located on the side of the support bracket 102 near the scale sticker 112. There are two scale stickers 112, which are adhered to the corresponding two rotating disks 104. The scale sticker 112 has scale values. There are two pointers 113, which are welded to the corresponding two support brackets 102. The pointers 113 can indicate the scale values ​​on the scale sticker 112. Thus, the scale sticker 112 and the pointers 113 make it easy for the operator to accurately read the flip angle of the cover glass connected to the rotating disk 104 rod.

[0029] Secondly, the limiting cylinder 114 is fixedly connected to the fixing frame 106 and is located on one side of the fixing frame 106; the limiting rod 115 is slidably connected to the limiting cylinder 114 and fixedly connected to the clamping frame 108, and is located on the side of the limiting cylinder 114 close to the clamping frame 108.

[0030] Meanwhile, the limiting ring 109 is fixedly connected to the rotating rod 103 and is located on the side of the rotating rod 103 near the support bracket 102. There are two limiting rings 109, which are welded to the rotating rod 103. The limiting rings 109 can prevent the rotating rod 103 from shifting or coming out of the support bracket 102 when it rotates inside the support bracket 102.

[0031] In addition, the rubber pad 110 is fixedly connected to the clamping frame 108 and is located on the side of the clamping frame 108 near the placement plate 105. There are two rubber pads 110, and the two rubber pads 110 are bonded to the corresponding two clamping frames 108. The rubber pads 110 can increase the friction force when the clamping frame 108 abuts against and clamps the cover glass slide, thereby making the cover glass slide more stable when flipping.

[0032] Finally, the locking bolt 111 is disposed on the support bracket 102 and abuts against the rotating rod 103. There are two locking bolts 111, which are disposed in the threaded holes of the support bracket 102. By turning the locking bolt 111 into the threaded holes of the support bracket 102, it can abut against the rotating rod 103 rotating in the support bracket 102, thereby locking the rotation of the rotating rod 103 when necessary.

[0033] When using the coverslip angle adjustment device and its method for use in a laser confocal microscope according to this embodiment, after the coverslip and slide are dripped with liquid, a detection blade is added and placed on the placement plate 105 for detection. When it is necessary to flip them, the operator moves the clamping frame 108 upwards, places the coverslip and slide on the placement plate 105, and releases the clamping frame 108 so that it returns to its original position under the deformation of the telescopic spring 107. Thus, the clamping frame 108, in conjunction with the placement plate 105, fixes the coverslip on the placement stage. The operator rotates the rotating disk 104 to drive the rotating rod 103 to rotate, thereby causing the placement platform to flip. The flipped placement plate 105 can then scan the cross-section of the blade in the cover glass slide on the placement plate 105. The clamping and fixing by the clamping frame 108 and the flipping of the placement plate 105 by the rotating rod 103 allow the operator to not only visually scan and inspect the surface of the blade, but also to scan the cross-section of the blade after flipping the cover glass slide on the placement plate 105.

[0034] The second embodiment of this application is as follows: Please see Figure 4 Based on the first embodiment, the cover slide angle adjustment device of the laser confocal microscope in this embodiment further includes a fixing component, which includes a first fixing ring 201 and a second fixing ring 202.

[0035] The first fixing ring 201 is fixedly connected to the fixing frame 106 and the telescopic spring 107 respectively; the second fixing ring 202 is fixedly connected to the clamping frame 108 and the telescopic spring 107 respectively. There are multiple first fixing rings 201, which are welded to two fixing frames 106 and to one end of the corresponding multiple telescopic springs 107. There are multiple second fixing rings 202, which are welded to two clamping frames 108 and to the other end of the corresponding multiple telescopic springs 107. Thus, the two ends of the telescopic spring 107 can be better connected and fixed to the fixing frame 106 and the clamping frame 108 through the first fixing ring 201 and the second fixing ring 202.

[0036] The third embodiment of this application is as follows: Please see Figure 5 Based on the second embodiment, the cover slide angle adjustment device of the laser confocal microscope in this embodiment further includes a moving component, which includes a moving frame 301 and a pull ring 302.

[0037] The movable frame 301 is fixedly connected to the clamping frame 108 and is located on one side of the clamping frame 108; the pull ring 302 is fixedly connected to the movable frame 301 and is located on the side of the movable frame 301 away from the clamping frame 108. There are two movable frames 301, which are welded to the corresponding two clamping frames 108. There are two pull rings 302, which are welded to the corresponding two movable frames 301. Thus, the pull rings 302 make it easier for the operator to move the clamping frame 108.

[0038] The fourth embodiment of this application is as follows: Based on the third embodiment, please refer to Figure 6 , Figure 6 This is a step diagram illustrating the method of using the coverslip angle adjustment device of the laser confocal microscope according to the third embodiment of the present invention.

[0039] The invention provides a method for using a coverslip angle adjustment device for a laser confocal microscope, comprising the following steps: S401: Fix the leaf sample in the center of the glass slide and cover it with a coverslip; S402: Move the clamping frame 108 to place the cover glass slide between the clamping frame 108 and the placement stage; S403: Release the clamping frame 108 so that the clamping frame 108 clamps and fixes the cover glass slide on the placement platform; S404: Turn on the microscope body 101 to scan the surface of the leaf sample in the cover slide; S405: Twist the rotating disk 104 to drive the placement platform to flip; S406: The cross-section of the sample in the flipped coverslip is photographed and scanned by the microscope body 101.

[0040] Place the cut leaf sample in the center of a clean glass slide, ensuring it is laid flat and free of wrinkles or tilting. Use a dropper to slowly add an appropriate amount of water or a specific fixative to the leaf sample, ensuring it is completely covered. Gently pick up a coverslip with tweezers, ensuring one edge of the coverslip contacts the liquid droplet on the slide first, then slowly lower the coverslip to gradually cover the leaf sample. Carefully move the prepared coverslip to the operating area and clamp it onto the placement plate 105 using the holder 108, ensuring the edges of the coverslip are flush with the holder 108. Align the edges of the stage so that the coverslip is centered in the microscope's field of view. Turn on the microscope body 101. Select the appropriate combination of objective and eyepiece lenses according to the observation needs. Adjust the microscope's focal length so that the surface of the leaf sample is clearly presented in the field of view. After observing and photographing the surface (back) of the leaf sample, when it is necessary to observe the cross-sectional structure of the leaf sample, rotate the rotating disk 104 to rotate the stage by 90 degrees so that the leaf sample presents a cross-sectional state. Adjust the microscope's focal length again so that the cross-section of the rotated leaf sample is clearly presented in the field of view for observation and photographing.

[0041] The above-disclosed embodiments are merely one or more preferred embodiments of this application and should not be construed as limiting the scope of this application. Those skilled in the art will understand that all or part of the processes for implementing the above embodiments and equivalent variations made in accordance with the claims of this application are still within the scope of this application.

Claims

1. A coverslip angle adjustment device for a laser confocal microscope, characterized in that, The device includes a support bracket, a rotating rod, a rotating disk, a placement plate, a fixing frame, a telescopic spring, a clamping frame, an indicating component, and a limiting component. The rotating rod is rotatably connected to the support bracket and located on one side of the support bracket. The rotating disk is fixedly connected to the rotating rod and located on one side of the rotating rod. The placement plate is fixedly connected to the rotating rod and located on the side of the rotating rod away from the rotating disk. The fixing frame is fixedly connected to the placement plate and located on one side of the placement plate. The telescopic spring is fixedly connected to the fixing frame and located on one side of the fixing frame. The clamping frame is connected to the fixing frame via the limiting component and is connected to the telescopic spring, and is located on the side of the fixing frame closer to the placement plate. The indicating component is disposed on the support bracket and connected to the rotating disk. The limiting component is disposed on the fixing frame and connected to the clamping frame.

2. The coverslip angle adjustment device for a laser confocal microscope as described in claim 1, characterized in that, The indicating component includes a scale sticker and a pointer. The scale sticker is fixedly connected to the rotating disk and located on one side of the rotating disk. The pointer is fixedly connected to the support bracket and located on the side of the support bracket closer to the scale sticker.

3. The coverslip angle adjustment device for a laser confocal microscope as described in claim 1, characterized in that, The limiting component includes a limiting cylinder and a limiting rod. The limiting cylinder is fixedly connected to the fixing frame and is located on one side of the fixing frame. The limiting rod is slidably connected to the limiting cylinder and fixedly connected to the clamping frame, and is located on the side of the limiting cylinder closer to the clamping frame.

4. The coverslip angle adjustment device for a laser confocal microscope as described in claim 1, characterized in that, The cover slide angle adjustment device of the laser confocal microscope also includes a limiting ring, which is fixedly connected to the rotating rod and located on the side of the rotating rod near the support bracket.

5. The coverslip angle adjustment device for a laser confocal microscope as described in claim 1 and its method of use, characterized in that, The cover slide angle adjustment device of the laser confocal microscope further includes a fixing component, which includes a first fixing ring and a second fixing ring. The first fixing ring is fixedly connected to the fixing frame and the telescopic spring, respectively; the second fixing ring is fixedly connected to the clamping frame and the telescopic spring, respectively.

6. The coverslip angle adjustment device for a laser confocal microscope as described in claim 1 and its method of use, characterized in that, The cover slide angle adjustment device of the laser confocal microscope further includes a moving component, which includes a moving frame and a pull ring. The moving frame is fixedly connected to the clamping frame and is located on one side of the clamping frame; the pull ring is fixedly connected to the moving frame and is located on the side of the moving frame away from the clamping frame.

7. A method of using a coverslip angle adjustment device for a laser confocal microscope, applicable to the coverslip angle adjustment device for a laser confocal microscope as described in any one of claims 1 to 6, characterized in that, Includes the following steps: Fix the leaf sample in the center of the glass slide and cover it with a coverslip; Move the clamp to place the cover glass slide between the clamp and the placement stage; Release the clamping frame to hold and fix the cover glass slide on the placement stage; Turn on the microscope body to scan the surface of the leaf sample in the cover slide; Rotate the rotating disk to cause the placement platform to flip; The microscope body is used to scan the cross-section of the sample in the flipped coverslip.

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