Efficient nano green bioengineering water-soluble fertilizer

Abstract

The invention discloses a high-efficiency nano green bioengineering water-soluble fertilizer, which relates to the field of fertilizers, and the active component of the high-efficiency nano green bioengineering water-soluble fertilizer is mainly composed of microbial metabolites and is obtained in a biological extraction mode; the water-soluble fertilizer comprises the following components in percentage by mass: 56.5% of organic matters and not less than 55.6% of total nutrients; the pH value of the water-soluble fertilizer is 8.7; the total nutrient content of the efficient nano green bioengineering water-soluble fertilizer is up to 55.6%, the organic matter content is up to 56.5%, meanwhile, the efficient nano green bioengineering water-soluble fertilizer is rich in multiple major elements, medium elements, trace elements and high-content amino acid, comprehensive and balanced nutrition can be provided for crops, and healthy growth of the crops is promoted.

Description

Technical Field

[0001] This invention relates to fertilizer technology, specifically to a high-efficiency nano-green bioengineering water-soluble fertilizer. Background Technology

[0002] Water-soluble fertilizer is a multi-component compound fertilizer that can be completely dissolved in water. After being dissolved or diluted in water, it can be used for irrigation fertilization, foliar fertilization, soilless cultivation, seed soaking and root dipping, etc. Its form includes liquid or solid. It can dissolve quickly in water without residue, so crops absorb it quickly and have a high utilization rate. It is especially suitable for use in facility agriculture in combination with micro-sprinkler irrigation and drip irrigation to achieve water and fertilizer integration, thereby saving water, fertilizer and labor.

[0003] With the pursuit of efficient, environmentally friendly, and sustainable production methods in modern agriculture, traditional chemical fertilizers, due to their low utilization rate and tendency to cause soil compaction and environmental pollution, are no longer sufficient to meet the needs of green agricultural development. Water-soluble fertilizers, as a new type of high-efficiency fertilizer, have been widely used due to their advantages such as good solubility, rapid nutrient absorption, and compatibility with irrigation systems. However, many existing water-soluble fertilizers still have some problems: First, some water-soluble fertilizers rely heavily on chemical synthesis, resulting in high energy consumption during production, and the products may contain chemicals that are unfriendly to soil microbial communities; second, some water-soluble fertilizers based on organic materials often have low nutrient content, especially in macro and mesonutrients, or unbalanced nutrient ratios, making it difficult to meet the comprehensive needs of crops throughout their entire growth cycle; third, micronutrients in some products exist mainly in the form of inorganic salts, which are easily fixed by the soil and have low bioavailability. Therefore, developing a high-efficiency water-soluble fertilizer with high nutrient content, balanced composition, easy absorption, and environmental friendliness has become an urgent technical problem to be solved in this field. Summary of the Invention

[0004] The purpose of this invention is to overcome the shortcomings of existing technologies and provide a highly efficient nano-green bioengineering water-soluble fertilizer. This water-soluble fertilizer uses microbial metabolites as its core and is obtained through bio-extraction technology. It has advantages such as comprehensive nutrients, high content, easily absorbed by crops, and environmental friendliness.

[0005] To achieve the above objectives, the present invention provides the following technical solution: a high-efficiency nano-green bioengineering water-soluble fertilizer, the active ingredients of which are mainly composed of microbial metabolites and obtained through biological extraction; the organic matter content of the water-soluble fertilizer is 56.5% by mass percentage, and the total nutrient content is not less than 55.6%; the pH value of the water-soluble fertilizer is 8.7.

[0006] Furthermore, the water-soluble fertilizer contains the following components at the following mass concentrations: total nitrogen (N) 0.6%, total potassium (K2O) 6.1%, zinc (Zn) 103.306 g / L, magnesium (Mg) 843 g / L, copper (Cu) 25.159 g / L, total phosphorus (P2O5) < 0.5%, manganese (Mn) 99.08 g / L, boron (B) 4.905 g / L, iron (Fe) 350 g / L, molybdenum (Mo) 0.2 g / L, cobalt (Co) 0.5 g / L, amino acids 250 g / L, calcium (Ca) 8391 mg / kg, and sulfur (S) 0.4%.

[0007] Furthermore, the microbial metabolites are derived from the fermentation products of a microbial community including Bacillus amyloliquefaciens and / or Bacillus jelly-like bacteria.

[0008] Furthermore, the trace elements in the water-soluble fertilizer exist in the form of organic chelates.

[0009] A method for preparing the above-mentioned high-efficiency nano-green bioengineering water-soluble fertilizer includes the following steps:

[0010] S1. High-density fermentation of functional microbial strains to obtain microbial fermentation broth;

[0011] S2. Perform membrane separation and concentration on the fermentation broth obtained in step S1 to obtain concentrated active liquid;

[0012] S3. Mix the concentrated active liquid with the mineral source and carry out a bio-activation and transformation reaction;

[0013] S4. Add the trace element organic chelate to the reaction product obtained in step S3 and mix well.

[0014] S5. The mixture obtained in step S4 is subjected to nano-scale pulverization and homogenization.

[0015] S6. Dry the material processed in step S5 to obtain the water-soluble fertilizer product.

[0016] Furthermore, the membrane separation described in step S2 includes microfiltration and ultrafiltration.

[0017] Furthermore, the nano-scale pulverization and homogenization process described in step S5 employs a high-pressure homogenizer to reduce the particle size of the solid particles in the material to the nanoscale.

[0018] Applications of high-efficiency nano-green bioengineering water-soluble fertilizers in increasing crop yield, improving crop quality, or improving soil.

[0019] Furthermore, the water-soluble fertilizer can be applied by foliar spraying, drip irrigation, or fertigation.

[0020] Furthermore, when used for foliar spraying, the water-soluble fertilizer is diluted 600-1000 times.

[0021] Compared with existing technologies, the high-efficiency nano-green bioengineering water-soluble fertilizer provided by this invention has the following beneficial effects:

[0022] I. The product of this invention has a total nutrient content of up to 55.6% and an organic matter content of 56.5%. It is also rich in a variety of macro, meso, and micro elements and high levels of amino acids, which can provide crops with comprehensive and balanced nutrition and promote healthy crop growth.

[0023] Second, the core of this fertilizer is microbial metabolites, in which nutrients are mostly in organic complex or chelate form. With the help of nanotechnology, the particle size is made smaller, making it easier for crop roots and leaves to absorb, thus greatly improving the utilization rate of nutrients.

[0024] Third, this product is prepared using a biological extraction method, and the production process is green and environmentally friendly. The product itself is rich in organic matter and beneficial microbial metabolites, which can effectively improve soil aggregate structure, stimulate the reproduction of beneficial microorganisms in the soil, enhance soil vitality, and long-term use can gradually repair degraded soil, which is in line with the development direction of green agriculture.

[0025] IV. The product has a weakly alkaline pH value and is stable. It can be mixed with most pesticides (except for strong acids and alkalis), making it convenient for various fertilization methods such as foliar spraying, drip irrigation, and fertigation, saving time and labor. Attached Figure Description

[0026] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in this invention. For those skilled in the art, other drawings can be obtained based on these drawings.

[0027] Figure 1 A diagram illustrating the preparation method of the high-efficiency nano-green bioengineering water-soluble fertilizer provided in this embodiment of the invention. Detailed Implementation

[0028] To enable those skilled in the art to better understand the technical solution of the present invention, the present invention will be further described in detail below with reference to the accompanying drawings.

[0029] Example 1:

[0030] A highly efficient nano-green bioengineering water-soluble fertilizer, the active ingredients of which are mainly composed of microbial metabolites and obtained through biological extraction; by mass percentage, the organic matter content of the water-soluble fertilizer is 56.5%, and the total nutrient content is not less than 55.6%; the pH value of the water-soluble fertilizer is 8.7.

[0031] Its active ingredients are mainly composed of microbial metabolites, which are obtained through bio-extraction. Utilizing the natural metabolic processes of microorganisms, it produces beneficial active substances for plants, such as plant hormones, enzymes, and antibiotics, thus providing a green and environmentally friendly fertilizer solution. The water-soluble fertilizer's organic matter content of 56.5% ensures abundant organic matter, which helps improve soil structure, increase soil water retention, and provide slow-release nutrients, promoting soil microbial activity. A total nutrient content of no less than 55.6% guarantees a high nutrient concentration, meeting the high demands of crops for macro-, meso-, and micronutrients, supporting comprehensive crop development. A pH of 8.7 indicates that the fertilizer is alkaline, which helps neutralize acidic soil, improves the availability of certain nutrients, and reduces soil heavy metal activity, thereby optimizing the crop growth environment.

[0032] The water-soluble fertilizer contains the following components at the following mass concentrations: total nitrogen (N) 0.6%, total potassium (K2O) 6.1%, zinc (Zn) 103.306 g / L, magnesium (Mg) 843 g / L, copper (Cu) 25.159 g / L, total phosphorus (P2O5) < 0.5%, manganese (Mn) 99.08 g / L, boron (B) 4.905 g / L, iron (Fe) 350 g / L, molybdenum (Mo) 0.2 g / L, cobalt (Co) 0.5 g / L, amino acids 250 g / L, calcium (Ca) 8391 mg / kg, and sulfur (S) 0.4%.

[0033] Containing various nutrients at specific mass concentrations, such as total nitrogen (0.6%) providing the basic nitrogen source needed for crop growth, promoting protein synthesis and chlorophyll formation; total potassium (6.1%) enhancing crop photosynthesis, water regulation, and stress resistance; zinc (103.306 g / L) participating in auxin synthesis as an enzyme activator; magnesium (843 g / L), a core component of chlorophyll supporting photosynthesis; copper (25.159 g / L) participating in redox reactions and enzyme function; total phosphorus (less than 0.5%) reducing the potential pollution risk of phosphorus to the environment while meeting the basic needs of crops; manganese (99.08 g / L) promoting photosynthesis and enzyme activity; boron (4.905 g / L) affecting cell division and pollen development; iron (350 g / L), key to chlorophyll synthesis and respiration; molybdenum (0.2 g / L) participating in nitrogen metabolism; cobalt (0.5 g / L) promoting vitamin B12 synthesis and nitrogen fixation; amino acids (250 g / L) providing crops with a directly absorbable organic nitrogen source, accelerating protein synthesis and cell repair; and calcium (8391 g / L). mg / kg strengthens cell wall structure and membrane stability, while sulfur (0.4%) is a component of proteins and vitamins. These elements work synergistically at precise concentrations to ensure crops receive comprehensive and balanced nutrition, effectively preventing nutrient deficiencies and improving crop health, yield, and quality.

[0034] The microbial metabolites are derived from the fermentation products of a microbial community including Bacillus amyloliquefaciens and / or Bacillus jelly-like bacteria.

[0035] Microbial metabolites originate from the fermentation products of Bacillus amyloliquefaciens and / or Bacillus colloidis microbial communities. These specific species produce a variety of substances that promote plant growth during fermentation, such as plant growth hormones, antimicrobial peptides, polysaccharides, and organic acids. This inhibits the growth of soil pathogens and reduces disease occurrence through the antagonistic effect of microorganisms. Furthermore, the metabolites stimulate crop root development, enhance nutrient absorption capacity and stress resistance, and improve the bioavailability and environmental friendliness of fertilizers. At the same time, these microbial communities can also decompose insoluble nutrients in the soil, increase nutrient availability, and achieve efficient fertilizer utilization and soil ecological improvement.

[0036] The trace elements in the water-soluble fertilizer exist in the form of organic chelates.

[0037] Micronutrients exist in the form of organic chelates. These organic chelates bind micronutrients such as zinc, iron, copper, and manganese to organic molecules such as amino acids or organic acids through coordination bonds, forming stable complexes. This form protects micronutrients from being fixed by phosphates, carbonates, or hydroxides in the soil, preventing nutrient loss and passivation. It also improves the mobility and bioavailability of micronutrients in the soil, making it easier for crop roots or leaves to absorb and utilize these elements. This effectively corrects nutrient deficiencies, enhances crop enzyme activity, photosynthetic efficiency, and stress resistance, while reducing fertilizer application frequency and environmental burden.

[0038] A method for preparing a high-efficiency nano-green bioengineering water-soluble fertilizer includes the following steps:

[0039] S1. High-density fermentation of functional microbial strains to obtain microbial fermentation broth;

[0040] S2. Perform membrane separation and concentration on the fermentation broth obtained in step S1 to obtain concentrated active liquid;

[0041] S3. Mix the concentrated active liquid with the mineral source and carry out a bio-activation and transformation reaction;

[0042] S4. Add the trace element organic chelate to the reaction product obtained in step S3 and mix well.

[0043] S5. The mixture obtained in step S4 is subjected to nano-scale pulverization and homogenization.

[0044] S6. Dry the material processed in step S5 to obtain the water-soluble fertilizer product.

[0045] Step S1 involves high-density fermentation of functional microbial strains to obtain a microbial fermentation broth, maximizing the yield of microbial metabolites by optimizing fermentation conditions such as temperature, pH, and aeration. Step S2 involves membrane separation and concentration of the fermentation broth to obtain a concentrated active liquid, removing impurities and retaining active ingredients through physical separation technology. Step S3 involves mixing the concentrated active liquid with a mineral source and conducting a bioactivation and transformation reaction, using microbial enzymes to convert the minerals into a more easily absorbed form. Step S4 involves adding trace element organic chelates to the reaction product and mixing them evenly to ensure the stability and uniform distribution of trace elements. Step S5 involves nano-scale pulverization and homogenization of the mixture to reduce particle size and increase specific surface area. Step S6 involves drying the treated material to obtain a water-soluble fertilizer product. This series of steps ensures the efficient extraction, purification, and retention of active ingredients, the effective integration of minerals, and the nano-scale characteristics of the fertilizer, thereby improving the fertilizer's solubility, stability, and crop absorption efficiency, achieving green and efficient fertilizer production.

[0046] The membrane separation described in step S2 includes microfiltration and ultrafiltration.

[0047] In S2, membrane separation includes microfiltration and ultrafiltration. Microfiltration uses membranes with larger pore sizes to remove large particulate impurities, bacteria, and suspended solids from the fermentation broth, achieving preliminary purification. Ultrafiltration uses membranes with smaller pore sizes to separate macromolecules such as proteins, polysaccharides, and colloids, while retaining small-molecule active metabolites. This purifies and concentrates microbial metabolites, ensuring the purity and concentration of active ingredients, while reducing the damage to heat-sensitive components. This provides a high-quality concentrated active liquid for subsequent steps, improving the efficacy and consistency of the fertilizer.

[0048] The nano-scale pulverization and homogenization process described in step S5 uses a high-pressure homogenizer to reduce the particle size of solid particles in the material to the nanoscale.

[0049] In step S5, the nano-scale pulverization and homogenization process uses a high-pressure homogenizer. The high-pressure homogenizer pressurizes the material through a narrow homogenization valve using a high-pressure pump, generating extremely high shear force, impact force, and cavitation effect, which breaks the solid particles down to the nanoscale. This significantly increases the specific surface area of ​​the fertilizer, improves its solubility, dispersibility, and stability in water, thereby enhancing the crop's nutrient absorption efficiency and application effect. At the same time, nanoscale particles can more easily penetrate plant cell walls, promoting rapid nutrient transport and utilization, and improving the fertilizer's bioavailability and crop response.

[0050] Applications of high-efficiency nano-green bioengineering water-soluble fertilizers in increasing crop yield, improving crop quality, or improving soil.

[0051] The microbial metabolites and comprehensive nutrients in water-soluble fertilizers can directly promote crop growth, increase chlorophyll synthesis and photosynthetic efficiency, thereby increasing biomass and yield. At the same time, organic matter and beneficial microbial metabolites can improve crop quality, such as increasing sugar, protein and vitamin content, enhancing fruit color and storage resistance. In addition, the alkaline pH and organic content of water-soluble fertilizers help neutralize soil acidity, increase soil organic matter and aggregate structure, promote soil microbial diversity and activity, inhibit pathogens, improve soil fertility and water retention capacity, and achieve sustainable soil utilization and ecological environment improvement.

[0052] The water-soluble fertilizer can be applied by foliar spraying, drip irrigation, or fertigation.

[0053] Application methods include foliar spraying, drip irrigation, or fertigation. Foliar spraying allows nutrients to be absorbed directly through leaf stomata and the cuticle, quickly correcting nutrient deficiencies and providing immediate nutritional support. Drip irrigation delivers fertilizer solution directly to the crop root zone, improving water and nutrient use efficiency and reducing evaporation and loss. Fertigation distributes fertilizer evenly across the soil surface using irrigation water, suitable for large-scale fertilization, ensuring nutrient penetration and root absorption. These multiple application methods provide flexibility to adapt to different crop types, growth stages, and field conditions, maximizing fertilizer effectiveness and convenience while reducing labor costs and environmental impact.

[0054] When used for foliar spraying, the water-soluble fertilizer should be diluted 600-1000 times.

[0055] The dilution range is based on the fertilizer's nutrient concentration and crop tolerance, ensuring that the fertilizer solution is within a safe and effective range. This avoids excessively high concentrations that could cause leaf burn, stomatal blockage, or metabolic disorders, while also preventing excessively low concentrations from affecting nutrient supply and effectiveness. The optimized dilution ratio allows nutrients to quickly penetrate leaf tissues, promoting efficient absorption and translocation, thereby enhancing crop photosynthesis, stress resistance, and yield, and ensuring optimal efficiency and crop safety for foliar fertilization.

[0056] Example 2:

[0057] This embodiment provides a method for preparing a highly efficient nano-green bioengineering water-soluble fertilizer based on Embodiment 1:

[0058] First, specific functional strains (such as *Bacillus amyloliquefaciens* and *Bacillus mucilaginosus*) are selected for high-density fermentation. By controlling fermentation conditions (such as temperature, pH, dissolved oxygen, and culture medium composition), the microorganisms are encouraged to grow in large quantities and produce metabolic products rich in amino acids, organic acids, polysaccharides, and various enzymes. After fermentation, the fermentation broth is initially purified and concentrated using membrane separation technologies such as microfiltration and ultrafiltration to remove bacterial cells and large molecular impurities while retaining active small molecules. Subsequently, the concentrated broth is transferred to a bioreactor, and natural mineral sources rich in calcium, magnesium, and sulfur are added according to a preset ratio. A microbial community with bioextraction capabilities is then inoculated, and a bioactivation and transformation reaction is carried out under suitable conditions, allowing the elements in the minerals to be effectively decomposed and complexed by the microorganisms. After the reaction, a fixed amount of organic chelates of zinc, copper, manganese, boron, iron, molybdenum, and cobalt (such as EDTA chelates and amino acid chelates) are added to the system and mixed uniformly. Finally, the mixed slurry is treated using a high-pressure homogenizer and nano-pulverization technology to disperse the solid particles at the nanoscale. After low-temperature spray drying, a powdered, high-efficiency nano-green bioengineering water-soluble fertilizer is obtained. The product was tested and found to contain 56.5% organic matter, 55.6% total nutrients, and a pH of 8.7. Its specific components are: total nitrogen (N) 0.6%, total potassium (K₂O) 6.1%, zinc (Zn) 103.306 g / L, magnesium (Mg) 843 g / L, copper (Cu) 25.159 g / L, total phosphorus (P₂O₅) < 0.5%, manganese (Mn) 99.08 g / L, boron (B) 4.905 g / L, iron (Fe) 350 g / L, molybdenum (Mo) 0.2 g / L, cobalt (Co) 0.5 g / L, amino acids 250 g / L, calcium (Ca) 8391 mg / kg, and sulfur (S) 0.4%.

[0059] Example 3:

[0060] This embodiment provides a method for preparing a highly efficient nano-green bioengineering water-soluble fertilizer based on Embodiment 2:

[0061] The steps are basically the same as in Example 2, except that the microbial strain used is a complex of Trichoderma harzianum and Saccharomyces cerevisiae, and the mineral source used in the bioactivation stage is a mixture of seaweed powder and oyster shell powder. The final water-soluble fertilizer product has an organic matter content of 55.8%, a total nutrient content of 55.0%, and a pH value of 8.5. The content of each component is similar to that in Example 2, and all are within the range defined in this scheme.

[0062] Example 4:

[0063] This embodiment provides a method for preparing a highly efficient nano-green bioengineering water-soluble fertilizer based on Embodiment 2:

[0064] The preparation method is the same as in Example 2, but after nano-scale pulverization and homogenization, spray drying is not performed. Instead, appropriate amounts of water, stabilizers, and suspending agents are added, and a stable suspension is formed by high-speed shearing. This liquid formulation is easy to dilute and use directly, and its active ingredients and content are equivalent to those of the powder product.

[0065] Example 5:

[0066] This embodiment demonstrates the application effect of the high-efficiency nano-green bioengineering water-soluble fertilizer prepared in Example 2 on tomato cultivation.

[0067] In a greenhouse where long-term cultivation had led to a decline in soil fertility, tomato seedlings were transplanted. At the initial flowering, fruit enlargement, and color-changing stages, this water-soluble fertilizer was diluted 800 times and applied via foliar spraying and drip irrigation, respectively. For drip irrigation, the dosage was 5 kg per acre per application. Results showed that, compared to a control group using conventional water-soluble fertilizer of equal nutrient value, tomato plants using this invention exhibited vigorous growth, thicker, darker green leaves, and a 15.3% increase in flowering and fruit setting rate. The fruits were uniform in size, bright in color, and showed an approximately 10% increase in vitamin C content and an approximately 8% increase in soluble solids content. Simultaneously, soil looseness and water retention were significantly improved. No obvious nutrient deficiency symptoms occurred throughout the entire growth period.

[0068] Example 6:

[0069] This embodiment demonstrates the application effect of the high-efficiency nano-green bioengineering water-soluble fertilizer prepared in Example 2 on rice.

[0070] During the early tillering and booting stages of rice, dilute this water-soluble fertilizer 1000 times and apply it as a foliar spray, using 50 kg of diluted solution per acre per application.

[0071] The results showed that, compared with the control group which used ordinary compound fertilizer, the experimental group of rice had an 8.5% increase in the number of effective tillers, a significant increase in leaf chlorophyll content, a 5.1% increase in thousand-grain weight, and an 11.7% increase in yield. Moreover, the rice in the experimental group had a lower content of heavy metals than the control group, indicating better quality.

[0072] Comparative Example 1:

[0073] A commercially available general-purpose chemically synthesized water-soluble fertilizer with a total nutrient content ≥55% was selected. Its nitrogen, phosphorus, and potassium ratio was similar to that of the product of this invention, but it did not contain organic matter, and the trace elements were in the form of inorganic salts. A comparative experiment was conducted under the same tomato cultivation conditions as in Example 5.

[0074] The results showed that although the tomatoes grew well in the early stages, they were prone to premature aging in the middle and later stages, resulting in a bland fruit flavor and slightly exacerbated soil compaction. Soil microbial diversity testing indicated that the soil microbial community richness was lower in soils treated with this chemical water-soluble fertilizer than in soils treated with the product of this invention.

[0075] Comparative Example 2:

[0076] A commercially available organic water-soluble fertilizer with potassium humate as the main raw material was selected. Its organic matter content was ≥50%, but its total nutrient content (N+K2O) was only 15%. Under the same tomato cultivation conditions as in Example 5, several times the dosage of this invention was required to achieve a similar nutrient supply level. This not only increased costs and labor intensity, but also, due to its excessively high potassium content and relative deficiency of nitrogen and micronutrients, led to a nutritional imbalance in the crop, causing excessive vegetative growth and a decrease in fruit set.

[0077] The above examples and comparative examples fully demonstrate that the high-efficiency nano-green bioengineering water-soluble fertilizer provided by the present invention is significantly superior to existing chemical water-soluble fertilizers or ordinary organic water-soluble fertilizers in terms of nutrient comprehensiveness, effectiveness, crop yield and quality improvement effects, and soil friendliness, with obvious comprehensive advantages.

[0078] The foregoing has only described certain exemplary embodiments of the present invention by way of illustration. Undoubtedly, those skilled in the art can modify the described embodiments in various ways without departing from the spirit and scope of the present invention. Therefore, the foregoing drawings and descriptions are illustrative in nature and should not be construed as limiting the scope of protection of the claims of the present invention.

Claims

1. A high-efficiency nano-green bioengineering water-soluble fertilizer, characterized in that, Its active ingredients are mainly composed of microbial metabolites, obtained through biological extraction; by mass percentage, the organic matter content of the water-soluble fertilizer is 56.5%, and the total nutrient content is not less than 55.6%; the pH value of the water-soluble fertilizer is 8.

7.

2. The high-efficiency nano-green bioengineering water-soluble fertilizer according to claim 1, characterized in that, The water-soluble fertilizer contains the following components at the following mass concentrations: total nitrogen (N) 0.6%, total potassium (K2O) 6.1%, zinc (Zn) 103.306 g / L, magnesium (Mg) 843 g / L, copper (Cu) 25.159 g / L, total phosphorus (P2O5) < 0.5%, manganese (Mn) 99.08 g / L, boron (B) 4.905 g / L, iron (Fe) 350 g / L, molybdenum (Mo) 0.2 g / L, cobalt (Co) 0.5 g / L, amino acids 250 g / L, calcium (Ca) 8391 mg / kg, and sulfur (S) 0.4%.

3. The high-efficiency nano-green bioengineering water-soluble fertilizer according to claim 1, characterized in that, The microbial metabolites are derived from the fermentation products of a microbial community including Bacillus amyloliquefaciens and / or Bacillus jelly-like bacteria.

4. The high-efficiency nano-green bioengineering water-soluble fertilizer according to claim 1, characterized in that, The trace elements in the water-soluble fertilizer exist in the form of organic chelates.

5. A method for preparing a high-efficiency nano-green bioengineering water-soluble fertilizer as described in any one of claims 1 to 4, characterized in that, Includes the following steps: S1. High-density fermentation of functional microbial strains to obtain microbial fermentation broth; S2. Perform membrane separation and concentration on the fermentation broth obtained in step S1 to obtain concentrated active liquid; S3. Mix the concentrated active liquid with the mineral source and carry out a bio-activation and transformation reaction; S4. Add the trace element organic chelate to the reaction product obtained in step S3 and mix well. S5. The mixture obtained in step S4 is subjected to nano-scale pulverization and homogenization. S6. Dry the material processed in step S5 to obtain the water-soluble fertilizer product.

6. The method for preparing a high-efficiency nano-green bioengineering water-soluble fertilizer according to claim 5, characterized in that, The membrane separation described in step S2 includes microfiltration and ultrafiltration.

7. The method for preparing a high-efficiency nano-green bioengineering water-soluble fertilizer according to claim 5, characterized in that, The nano-scale pulverization and homogenization process described in step S5 uses a high-pressure homogenizer to reduce the particle size of solid particles in the material to the nanoscale.

8. The application of the high-efficiency nano-green bioengineering water-soluble fertilizer according to any one of claims 1-4 in improving crop yield, crop quality, or soil.

9. The application according to claim 8, characterized in that, The water-soluble fertilizer can be applied by foliar spraying, drip irrigation, or fertigation.

10. The application according to claim 9, characterized in that, When used for foliar spraying, the water-soluble fertilizer should be diluted 600-1000 times.

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