Anti-tumor drug screening device based on in-vitro cell culture

Abstract

The invention discloses an anti-tumor drug screening device based on in-vitro cell culture, and particularly relates to the technical field of drug screening, the anti-tumor drug screening device comprises a device base, a first mounting hole is formed in the upper end of the device base, a plurality of L-shaped rods are arranged on the outer surface of the device base in an array mode, and a first mounting plate is jointly arranged at the upper ends of the vertical parts of the L-shaped rods; an electric push rod is arranged in the middle of the lower end of the first mounting plate, a cleaning brush is arranged at the output end of the electric push rod, a first supporting plate is jointly arranged among the L-shaped rods, and a second mounting hole matched with the first mounting hole is formed in the upper end of the first supporting plate; and a screening device is arranged between the first mounting hole and the second mounting hole. According to the anti-tumor drug screening device based on in-vitro cell culture, the design of the screening device is adopted, so that the device can adjust the proportion of discharged water to drugs according to different drugs, and meanwhile, the device is convenient to operate.

Description

Technical Field

[0001] This invention relates to the field of drug screening technology, and in particular to a device for screening antitumor drugs based on in vitro cell culture. Background Technology

[0002] Cell culture refers to a method of simulating the in vivo environment to enable cells to survive, grow, reproduce, and maintain their main structures and functions. Cell culture is also called cell cloning technology, and the formal term in biology is cell culture technique. Whether for the entire field of bioengineering or for biological cloning, cell culture is an indispensable process; it is essentially the large-scale cloning of cells. Cell culture technology is an important and commonly used technique in cell biology research. Through cell culture, large numbers of cells can be obtained, and these cells can be used to study cell signal transduction, cellular synthesis and metabolism, cell growth and proliferation, etc. Cell culture requires the provision of drugs to provide the nutrients needed for cell growth, and these drugs are mainly composed of powdered drugs mixed with water.

[0003] Chinese patent document CN110118863A discloses a high-throughput automated drug screening device and method, including a three-dimensional moving device, a gas path system, and an electrostatic field on / off control system. The three-dimensional moving device includes four motor modules, a nozzle, and a base platform arranged along the X, Y, and Z axes. The base platform is slidably mounted on the upper surface of the Y-axis motor module, and the nozzle is slidably mounted on the front end face of the X-axis motor module, directly facing the base platform. The three-dimensional moving device is controlled by a displacement control system. The nozzle is connected to the gas path system. The nozzle and base platform are controlled by the electrostatic field on / off control system. The displacement control system, gas path system, and electrostatic field on / off control system are controlled by a host computer control system. This device achieves automated control, reduces workload, minimizes operational errors, and improves screening efficiency. The high-voltage electric field microdroplet generation technology reduces drug consumption and significantly reduces experimental costs, thus meeting the needs of rapid, high-efficiency, and micro-quantity drug screening technology.

[0004] The device cannot adjust the ratio of discharged water to drug according to different drugs, which makes it unsuitable for use and inconvenient to operate. Summary of the Invention

[0005] The main objective of this invention is to provide a device for screening anti-tumor drugs based on in vitro cell culture, which can effectively solve the problems of poor applicability and inconvenience in operation of the device.

[0006] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0007] A device for screening antitumor drugs based on in vitro cell culture includes a device base with a first mounting hole at the upper end. Multiple L-shaped rods are arrayed on the outer surface of the device base. A first mounting plate is provided at the upper end of the vertical sections of the multiple L-shaped rods. An electric actuator is provided at the middle of the lower end of the first mounting plate. A cleaning brush is provided at the output end of the electric actuator. A first support plate is provided between the multiple L-shaped rods. A second mounting hole adapted to the first mounting hole is provided at the upper end of the first support plate. A screening device is provided between the first mounting hole and the second mounting hole.

[0008] Preferably, the screening device includes a screening barrel, a first external toothed ring is provided on the upper part of the outer surface of the screening barrel, a drive motor is provided on the upper part of the first support plate, a first transmission gear adapted to the first external toothed ring is provided at the output end of the drive motor, discharge pipes are provided on both the upper and lower sides of the outer surface of the screening barrel, and the discharge pipes communicate with the interior of the screening barrel, a sealing block adapted to the screening barrel is provided in the inner cavity of the discharge pipe, and an adjustment device is provided at the lower end of the screening barrel.

[0009] Preferably, the outer surface of the screening bucket is in contact with the inner surface of the second mounting hole and the inner surface of the first mounting hole.

[0010] Preferably, the sealing block is made of a flexible soft material, the end of the discharge pipe away from the center of the screening barrel is provided with a fixing nut with a notch, and the end of the sealing block away from the center of the screening barrel is provided with a fixing screw that matches the fixing nut.

[0011] Preferably, the adjusting device includes multiple sets of first limiting blocks, and the multiple sets of first limiting blocks are arranged in a ring on the bottom surface of the inner cavity of the screening barrel. Each set of first limiting blocks has two blocks. A first support rod is provided between the two first limiting blocks in the same set. An inner toothed ring is provided at the lower end of the screening barrel. An adjusting handle is provided on the outer surface of the inner toothed ring. Multiple second support rods are arranged in an array at the lower end of the screening barrel. An adjusting mechanism is provided at the lower end of the multiple second support rods. An adjusting membrane is provided on the lower part of the inner surface of the screening barrel.

[0012] Preferably, the regulating membrane is an elastic isolation membrane, and the plurality of first support rods are located at the lower part of the regulating membrane, and the side of the plurality of first support rods near the center of the screening barrel is in contact with the bottom surface of the regulating membrane.

[0013] Preferably, the adjustment mechanism includes a second mounting plate, the lower end of the second mounting plate is provided with a plurality of limiting grooves corresponding to the first support rod, the inner cavity of the limiting groove is provided with an adjustment screw, the lower end of the screening barrel is provided with a plurality of second external toothed rings adapted to the adjustment screw, and the plurality of second external toothed rings are engaged with the inner toothed ring.

[0014] Preferably, when the regulating membrane is in its natural state, the lowest point of the connection between the discharge pipe and the screening barrel located below is at the same level as the top surface of the inner cavity of the regulating membrane.

[0015] Preferably, the connection between the two is a sliding connection, the connection between the two is a threaded connection, and the upper part penetrates through the bottom wall of the inner cavity.

[0016] Compared with the prior art, the present invention has the following beneficial effects:

[0017] This invention employs a screening device design. Through the interaction between an inner toothed ring and a second outer toothed ring, the rotation of the inner toothed ring drives multiple second outer toothed rings to rotate. The interaction between the second outer toothed rings, the adjusting screw, and the limiting groove causes the adjusting screw to slide upwards when the second outer toothed rings rotate. The interaction between the adjusting screw and the first support rod causes the adjusting membrane to deform, thereby adjusting the volume of the screening barrel located between the two discharge pipes. This allows the device to adjust the ratio of water to drug discharged according to different drugs. The interaction between the first transmission gear and the first outer toothed ring causes the screening barrel to centrifugally rotate the drug, separating the water and drug from the liquid drug, facilitating the operation of the device. Attached Figure Description

[0018] Figure 1 This is a schematic diagram of the overall structure of the present invention;

[0019] Figure 2 This is a partial structural schematic diagram of the device of the present invention;

[0020] Figure 3 This is a schematic diagram of the screening device of the present invention;

[0021] Figure 4 For the present invention Figure 1 Enlarged view of point A in the middle;

[0022] Figure 5 This is a partial structural diagram of the adjusting device of the present invention. Figure 1 ;

[0023] Figure 6 This is a partial structural diagram of the adjusting device of the present invention. Figure 2 ;

[0024] Figure 7 For the present invention Figure 6 Enlarged view of point B in the middle;

[0025] Figure 8 This is an exploded view of the regulating device of the present invention;

[0026] Figure 9 For the present invention Figure 8 Enlarged view of point C in the middle;

[0027] Figure 10 This is a plan view of the device of the present invention in use.

[0028] In the diagram: 1. Device base; 2. Screening device; 3. Adjusting device; 11. First mounting hole; 12. L-shaped rod; 13. First mounting plate; 14. Electric push rod; 15. Cleaning brush; 16. First support plate; 17. Second mounting hole; 21. Screening barrel; 22. First external gear ring; 23. Drive motor; 24. First transmission gear; 25. Discharge pipe; 26. Sealing block; 261. Fixing nut; 262. Fixing screw; 31. First limiting block; 32. First support rod; 33. Internal gear ring; 34. Adjusting handle; 35. Second support rod; 36. Adjusting mechanism; 361. Second mounting plate; 362. Limiting groove; 363. Adjusting screw; 364. Second external gear ring; 37. Adjusting membrane. Detailed Implementation

[0029] To make the technical means, creative features, objectives and effects of this invention easier to understand, the invention will be further described below in conjunction with specific embodiments.

[0030] like Figure 1-10 As shown, an antitumor drug screening device based on in vitro cell culture includes a device base 1. A first mounting hole 11 is provided at the upper end of the device base 1. Multiple L-shaped rods 12 are arrayed on the outer surface of the device base 1. The device base 1 and the multiple L-shaped rods 12 are all fixedly connected. A first mounting plate 13 is provided at the upper end of the vertical portion of the multiple L-shaped rods 12. The first mounting plate 13 is also fixedly connected to the multiple L-shaped rods 12. The first mounting plate 13 is used for mounting an electric actuator 14. The electric actuator 14 is located at the lower center of the first mounting plate 13. The connection between the first mounting plate 13 and the electric actuator 14 is fixedly connected. The electric actuator 14 is a prior art technology. The electric actuator 14 has a cleaning brush 15 at its output end. The connection between the output end of the electric actuator 14 and the cleaning brush 15 is fixed. The cleaning brush 15 is existing technology and is used to clean the screening barrel 21 after use. It is also used to seal the screening barrel 21 when it is rotating at high speed to prevent the screening barrel 21 from throwing out the drug when it is rotating at high speed. A first support plate 16 is provided between multiple L-shaped rods 12. The connection between the first support plate 16 and multiple L-shaped rods 12 is fixed. A second mounting hole 17 adapted to the first mounting hole 11 is opened at the upper end of the first support plate 16. The screening device 2 is provided between the first mounting hole 11 and the second mounting hole 17.

[0031] Furthermore, to enable the device to centrifuge liquid drugs during use, removing water from the drugs, and facilitating the recovery of diluted drugs to reduce waste, such as... Figure 2-10 As shown, the screening device 2 includes a screening barrel 21. The outer surface of the screening barrel 21 is in contact with the inner surface of the second mounting hole 17 and the inner surface of the first mounting hole 11. The screening barrel 21 is rotatably connected to the first mounting hole 11 and the second mounting hole 17. The first mounting hole 11 and the second mounting hole 17 are used to support the screening barrel 21. A first external toothed ring 22 is provided on the upper part of the outer surface of the screening barrel 21. The screening barrel 21 is fixedly connected to the first external toothed ring 22. A drive motor 23 is provided on the upper end of the first support plate 16. The first support plate 16 is fixedly connected to the drive motor 23. 23 is existing technology, and the drive motor 23 is a high-speed motor that can drive the screening barrel 21 to rotate at high speed, so that the screening barrel 21 can centrifuge the liquid drug when rotating at high speed. The output end of the drive motor 23 is provided with a first transmission gear 24 that is adapted to the first external gear ring 22. The connection between the output end of the drive motor 23 and the first transmission gear 24 is a fixed connection. The first transmission gear 24 and the first external gear ring 22 mesh with each other, so that when the drive motor 23 rotates, it drives the screening barrel 21 to rotate, thereby facilitating the centrifugation of the liquid drug in the screening barrel 21 when it rotates.

[0032] Specifically, centrifugation is a method of separating substances with different specific gravities using centrifugal force. Because centrifuges and similar equipment can generate very high angular velocities, the centrifugal force is much greater than gravity, making it easier for suspended solids in a solution to precipitate. Furthermore, since substances with different specific gravities experience different centrifugal forces, they settle at different rates, thus achieving separation. Centrifugation is the most commonly used biochemical separation method for biomolecules because different biomolecules have different volumes and densities, allowing them to settle and separate under varying centrifugal forces. With the development of life sciences and technologies, centrifugation has become an indispensable separation technique in biochemistry and molecular biology. Centrifugation allows diluted drugs to be separated from water, facilitating drug recovery and reuse.

[0033] Specifically, the screening barrel 21 has discharge pipes 25 on both the upper and lower sides of its outer surface, and the discharge pipes 25 are connected to the interior of the screening barrel 21. The connection between the screening barrel 21 and the discharge pipes 25 is a fixed connection. The two discharge pipes 25 are used to discharge the drug and water respectively. The upper discharge pipe 25 is used to discharge the drug or water with lower density after high-speed centrifugation, and the lower discharge pipe 25 is used to discharge the drug or water with higher density after high-speed centrifugation. The inner cavity of the discharge pipe 25 is provided with a sealing block 26 that is compatible with the screening barrel 21. The connection between the sealing block 26 and the discharge pipe 25 is a sliding connection. The lower end of the screening barrel 21 is provided with an adjustment device 3.

[0034] Furthermore, in order to seal the discharge pipe 25 during use, such as... Figure 3-10 As shown, the sealing block 26 is made of a flexible, soft material, including but not limited to elastic rubber, silicone, latex, etc., used to seal the discharge pipe 25. It also pushes residual medicine or water from the inner wall of the discharge pipe 25 into the screening tank 21, allowing the cleaning brush 15 to remove the medicine or water pushed into the screening tank 21 during cleaning. A notched fixing nut 261 is provided at the end of the discharge pipe 25 furthest from the center of the screening tank 21. The connection between the fixing nut 261 and the discharge pipe 25 is a fixed connection. The sealing block 26 is provided at the end furthest from the center of the screening tank 21... The fixing nut 261 is matched with the fixing screw 262. The connection between the sealing block 26 and the fixing screw 262 is a rotatable connection, while the connection between the fixing nut 261 and the fixing screw 262 is a threaded connection. The fixing nut 261 and the fixing screw 262 cooperate with each other to fix the sealing block 26, preventing the sealing block 26 from failing to seal the discharge pipe 25 when the screening barrel 21 is rotating at high speed, thus preventing the medicine liquid in the screening barrel 21 from flowing out. At the same time, the fixing nut 261 adopts a notch design to prevent medicine liquid or water from entering the thread when discharged, thereby causing contamination of the fixing nut 261.

[0035] Furthermore, to allow the device to adjust the ratio of discharged water to drug according to different drugs during use, such as... Figure 5-10As shown, the adjusting device 3 includes multiple sets of first limiting blocks 31, which are arranged in a ring on the bottom surface of the inner cavity of the screening barrel 21. Each set of first limiting blocks 31 consists of two blocks. The connection between the multiple first limiting blocks 31 and the screening barrel 21 is a fixed connection. The two first limiting blocks 31 in the same set are used to support the first support rod 32. The two first limiting blocks 31 in the same set share the first support rod 32. The connection between the two first limiting blocks 31 in the same set and the first support rod 32 is a rotatable connection. The multiple first support rods 32 cooperate with each other to deform the adjusting membrane 37, thereby adjusting the volume of the screening barrel 21 located between the two discharge pipes 25. This allows the device to adjust the ratio of water to drug discharged according to different drugs.

[0036] Specifically, the lower end of the screening barrel 21 is provided with an internal toothed ring 33. The connection between the screening barrel 21 and the internal toothed ring 33 is a rotatable connection. The internal toothed ring 33 is used to drive multiple second external toothed rings 364 to rotate. The outer surface of the internal toothed ring 33 is provided with an adjustment handle 34. The connection between the internal toothed ring 33 and the adjustment handle 34 is a fixed connection. The adjustment handle 34 is used to drive the internal toothed ring 33 to rotate. The lower end of the screening barrel 21 is provided with multiple second support rods 35. The connection between the screening barrel 21 and the multiple second support rods 35 is a fixed connection. The multiple second support rods 35 are used to support the second mounting plate 361 to facilitate the installation of the second mounting plate 361. The lower ends of the multiple second support rods 35 are provided with an adjustment mechanism 36. The lower part of the inner surface of the screening barrel 21 is provided with an adjustment membrane 37. The connection between the inner surface of the screening barrel 21 and the outer surface of the adjustment membrane 37 is a fixed connection.

[0037] Specifically, the regulating membrane 37 is an elastic isolation membrane, including but not limited to elastic rubber, silicone, latex, etc., to prevent drug leakage. At the same time, the regulating membrane 37 can cooperate with the first support rod 32, so that when the first support rod 32 rotates upward or downward, the regulating membrane 37 can deform. Multiple first support rods 32 are located at the lower part of the regulating membrane 37, and the side of multiple first support rods 32 near the center of the screening barrel 21 is in contact with the bottom surface of the regulating membrane 37. When multiple first support rods 32 rotate upward, the regulating membrane 37 can deform, thereby adjusting the volume of the screening barrel 21 located between the two discharge pipes 25. This allows the device to adjust the ratio of discharged water to drug according to different drugs.

[0038] When the regulating membrane 37 is in its natural state, the lowest point of the connection between the discharge pipe 25 and the screening barrel 21 is at the same level as the top surface of the inner cavity of the regulating membrane 37, so that the liquid in the screening barrel 21 can be completely discharged, preventing the liquid in the screening barrel 21 or the cleaning liquid after cleaning from not being completely discharged, which would cause contamination to other drugs when the device is used again.

[0039] Furthermore, to facilitate the adjustment of the regulating membrane 37, such as... Figure 3-10 As shown, the adjustment mechanism 36 includes a second mounting plate 361. The second mounting plate 361 and the multiple second support rods 35 are all fixedly connected. The lower end of the second mounting plate 361 has multiple limiting grooves 362 corresponding to the first support rods 32. The inner cavity of the limiting groove 362 is provided with an adjusting screw 363. The limiting groove 362 and the adjusting screw 363 are slidably connected. The adjusting screw 363 extends upward through the bottom wall of the inner cavity of the screening barrel 21. The distance between the adjusting screw 363 and the screening barrel 21 is... The connection is a sliding connection. The lower end of the screening barrel 21 is arrayed with multiple second external toothed rings 364 that are adapted to the adjusting screw 363. All the second external toothed rings 364 mesh with the internal toothed ring 33. The connection between the second external toothed rings 364 and the adjusting screw 363 is a threaded connection. When the internal toothed ring 33 drives the second external toothed rings 364 to rotate, the adjusting screw 363 can move up or down relative to the screening barrel 21, which further causes the first support rod 32 to rotate up or down.

[0040] The specific implementation method is as follows: When using the device, firstly, according to different drugs, push the adjusting handle 34 to rotate the internal gear ring 33. The internal gear ring 33, through its interaction with multiple second external gear rings 364, causes the multiple second external gear rings 364 to rotate simultaneously. The second external gear rings 364, through their interaction with the adjusting screws 363, cause the multiple adjusting screws 363 to move upward or downward. When the adjusting screws 363 move upward or downward, through their interaction with the first support rod 32, the first support rod 32 rotates upward or downward, further... The ground causes the regulating membrane 37 to deform, thereby adjusting the volume of the screening barrel 21 located between the two discharge pipes 25. Then, the liquid medicine to be screened is poured into the screening barrel 21. Then, the electric push rod 14 extends, so that the cleaning brush 15 seals the screening barrel 21. The drive motor 23 is started, so that the first transmission gear 24 meshes with the first external gear ring 22, thereby driving the screening barrel 21 to rotate at high speed. When the screening barrel 21 rotates, it drives the medicine to rotate centrifugally, so that the water medicine in the liquid medicine is separated, which facilitates the recovery of the diluted medicine and reduces waste.

[0041] After the drug is centrifuged, the drive motor 23 stops rotating. First, rotate the upper fixing bolt 262, then remove the upper sealing block 26 to allow the liquid (water or drug solution) to drain out. Figure 10As shown at point E, the liquid at point E has a lower density. After the discharge pipe 25 stops discharging liquid, rotate the fixing bolt 262 below, and then remove the sealing block 26 below to allow the liquid (water or medicine) below to drain out. Figure 10 As shown at point F, where F is a liquid with a higher density, after the liquid in the screening tank 21 is completely discharged, the two discharge pipes 25 are sealed, and then cleaning liquid is added to the screening tank 21 to clean it. At the same time, the drive motor 23 rotates, further causing the screening tank 21 to rotate. When the screening tank 21 rotates, the electric push rod 14 extends, causing the cleaning brush 15 to clean the screening tank 21, and then the cleaning liquid in the cleaned screening tank 21 is discharged.

[0042] It should be noted that the specific installation and control methods of the electric actuator 14, cleaning brush 15, drive motor 23, etc. in this invention are all conventional designs in the prior art, and will not be described in detail in this invention.

[0043] The foregoing has shown and described the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of the invention. Various changes and modifications can be made to the invention without departing from its spirit and scope, and all such changes and modifications fall within the scope of the present invention as claimed. The scope of protection of this invention is defined by the appended claims and their equivalents.

Claims

1. A device for screening antitumor drugs based on in vitro cell culture, comprising a device base (1), characterized in that: The device base (1) has a first mounting hole (11) at its upper end. The outer surface of the device base (1) is provided with a plurality of L-shaped rods (12). The upper ends of the vertical parts of the plurality of L-shaped rods (12) are provided with a first mounting plate (13). The lower middle part of the first mounting plate (13) is provided with an electric push rod (14). The output end of the electric push rod (14) is provided with a cleaning brush (15). The plurality of L-shaped rods (12) are provided with a first support plate (16). The upper end of the first support plate (16) is provided with a second mounting hole (17) that is adapted to the first mounting hole (11). The first mounting hole (11) and the second mounting hole (17) are provided with a screening device (2).

2. The device for screening antitumor drugs based on in vitro cell culture according to claim 1, characterized in that: The screening device (2) includes a screening barrel (21). A first external toothed ring (22) is provided on the upper part of the outer surface of the screening barrel (21). A drive motor (23) is provided on the upper end of the first support plate (16). A first transmission gear (24) adapted to the first external toothed ring (22) is provided at the output end of the drive motor (23). Discharge pipes (25) are provided on both the upper and lower sides of the outer surface of the screening barrel (21). The discharge pipes (25) are connected to the inside of the screening barrel (21). A sealing block (26) adapted to the screening barrel (21) is provided in the inner cavity of the discharge pipe (25). An adjustment device (3) is provided at the lower end of the screening barrel (21).

3. The device for screening antitumor drugs based on in vitro cell culture according to claim 2, characterized in that: The outer surface of the screening bucket (21) is in contact with the inner surface of the second mounting hole (17) and the inner surface of the first mounting hole (11).

4. The device for screening antitumor drugs based on in vitro cell culture according to claim 2, characterized in that: The sealing block (26) is made of a flexible soft material. The end of the discharge pipe (25) away from the center of the screening barrel (21) is provided with a fixing nut (261) with a notch. The end of the sealing block (26) away from the center of the screening barrel (21) is provided with a fixing screw (262) that is compatible with the fixing nut (261).

5. The device for screening antitumor drugs based on in vitro cell culture according to claim 2, characterized in that: The adjusting device (3) includes multiple sets of first limiting blocks (31), and the multiple sets of first limiting blocks (31) are arranged in a ring on the bottom surface of the inner cavity of the screening barrel (21). Each set of first limiting blocks (31) has two blocks. A first support rod (32) is provided between the two first limiting blocks (31) in the same set. An internal toothed ring (33) is provided at the lower end of the screening barrel (21). An adjusting handle (34) is provided on the outer surface of the internal toothed ring (33). Multiple second support rods (35) are arranged in an array at the lower end of the screening barrel (21). An adjusting mechanism (36) is provided at the lower end of the multiple second support rods (35). An adjusting membrane (37) is provided on the lower part of the inner surface of the screening barrel (21).

6. The device for screening antitumor drugs based on in vitro cell culture according to claim 5, characterized in that: The regulating membrane (37) is an elastic isolation membrane. Multiple first support rods (32) are located at the lower part of the regulating membrane (37), and the side of the multiple first support rods (32) near the center of the screening barrel (21) is in contact with the bottom surface of the regulating membrane (37).

7. The device for screening antitumor drugs based on in vitro cell culture according to claim 5, characterized in that: The adjustment mechanism (36) includes a second mounting plate (361). The lower end of the second mounting plate (361) is provided with a plurality of limiting grooves (362) corresponding to the first support rod (32). The inner cavity of the limiting groove (362) is provided with an adjustment screw (363). The lower end of the screening barrel (21) is provided with a plurality of second external toothed rings (364) adapted to the adjustment screws (363), and the plurality of second external toothed rings (364) are engaged with the internal toothed ring (33).

8. The device for screening antitumor drugs based on in vitro cell culture according to claim 7, characterized in that: When the regulating membrane (37) is in its natural state, the lowest point of the connection between the discharge pipe (25) and the screening barrel (21) located below is at the same level as the top surface of the inner cavity of the regulating membrane (37).

9. The device for screening antitumor drugs based on in vitro cell culture according to claim 7, characterized in that: The connection between (363) and (362) is a sliding connection, and the connection between (363) and (364) is a threaded connection, with (363) extending upward through the bottom wall of the inner cavity of (21).

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