Print fading and repairing composition as well as preparation method and application thereof

Abstract

The invention relates to the technical field of cosmetics, in particular to a mark fading and repairing composition as well as a preparation method and application thereof. The invention relates to a mark fading and repairing composition which comprises the following components in percentage by weight: 0.1%-5% of dragon's blood extract, 0.01%-0.5% of keratinase and 1%-5% of nicotinamide, the preparation method comprises the following steps: preparing the eutectic solvent; performing ultrasonic-assisted extraction; separation and post-treatment; the invention has the following beneficial effects: the resina draconis extract can inhibit continuous pigmentation caused by acne inflammation residue, promote healing of skin minimal wounds and reduce dermis layer injury of acne mark parts; keratinase specifically hydrolyzes stratum corneum aged keratin, cutin metabolism of acne mark parts is accelerated, and accumulation of pigments in stratum corneum is avoided; nicotinamide inhibits melanocytes from transferring melanin to keratinocytes; through the synergistic effect of the three components, a closed-loop printing fading mechanism of anti-inflammatory repair-keratin metabolism-pigment inhibition is formed, the printing fading effect is more comprehensive and more efficient, and skin repair and barrier protection can be both considered.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, and in particular to a blemish repair composition, its preparation method, and its application. Background Technology

[0002] Redness (red acne marks) and pigmentation (dark brown acne marks) left after acne healing are common skin problems. Red acne marks are mainly related to post-inflammatory telangiectasia and damage to the skin barrier; dark brown acne marks are caused by inflammation stimulating melanocytes to produce excessive melanin. Currently, most products on the market that fade acne marks focus on a single pathway, such as using niacinamide to inhibit melanin transport or using acidic ingredients to accelerate keratin metabolism, often neglecting the simultaneous repair of the inflammatory microenvironment and the skin barrier, resulting in limited effectiveness and potential irritation. Summary of the Invention

[0003] To address the shortcomings of existing technologies, one objective of this invention is to provide a blemish-fading and repairing composition; another objective is to provide a method for preparing the composition; and a third objective is to provide the application of the composition in the preparation of cosmetics for fading acne scars and repairing the skin barrier.

[0004] One of the objectives of this invention is achieved through the following technical solution: A blemish repair composition comprising, by weight percentage, the following active ingredients: 0.1%-5% dragon's blood extract, 0.01%-0.5% keratinase and 1%-5% niacinamide.

[0005] Preferably, a blemish repair composition comprises, by weight percentage, the following active ingredients: 0.5%-3% dragon's blood extract, 0.05%-0.3% keratinase and 2%-4% niacinamide.

[0006] Preferably, the dragon's blood extract is obtained by ultrasound-assisted eutectic solvent extraction, wherein the eutectic solvent is composed of hydrogen bond acceptors and hydrogen bond donors, wherein the hydrogen bond acceptor is betaine and the hydrogen bond donor is a mixture of 1,3-butanediol and glycerol.

[0007] Preferably, in the eutectic solvent, the mass ratio of betaine, 1,3-butanediol, and glycerol is 1:(2-4):(1-3). The DES formed under this ratio has moderate viscosity and achieves the highest solubility and extraction rate for active ingredients such as flavonoids and phenols in dragon's blood.

[0008] Preferably, the composition further comprises a cosmetically acceptable carrier and auxiliary ingredients, and is formulated into dosage forms such as serum, toner, lotion, gel, or mask.

[0009] The second objective of this invention is achieved through the following technical solution: A method for preparing a blemish repair composition includes the following steps: Preparation of eutectic solvent: Betaine, 1,3-butanediol and glycerol are mixed in proportion and stirred at 60-80℃ until a homogeneous and transparent liquid is formed, thus obtaining the eutectic solvent.

[0010] Ultrasonic-assisted extraction: Mix the raw material powder of dragon's blood with the above-mentioned eutectic solvent at a material-to-liquid ratio of 1:10-1:30 (g / mL), place it in an ultrasonic device, and ultrasonically extract at 40-60℃ for 20-60 minutes.

[0011] Separation and post-processing: Centrifuge or filter the extract, and collect the supernatant to obtain the dragon's blood extract. This extract can be used directly in the preparation of compositions or after appropriate dilution.

[0012] Preferably, the mass ratio of 1,3-butanediol to glycerol is 3:2.

[0013] Preferably, the ultrasonic device is equipped with an ultrasonic power of 250W.

[0014] Preferably, the centrifugation speed is 9000 r / min and the centrifugation time is 12 min.

[0015] The third objective of this invention is achieved through the following technical solution: The application of a blemish-fading and repairing composition in the preparation of cosmetics for fading acne scars, improving post-inflammatory hyperpigmentation, and repairing the skin barrier.

[0016] The beneficial effects of this invention are as follows: Dragon's Blood Extract: Its flavonoid and phenolic active ingredients have strong anti-inflammatory and antioxidant effects, which can inhibit the persistent pigmentation caused by residual acne inflammation, while promoting the healing of micro-wounds in the skin, reducing dermal damage in acne scar areas, and laying the foundation for skin regeneration.

[0017] Keratinase: Specifically hydrolyzes aged keratin in the stratum corneum, accelerates keratin metabolism in acne scar areas, and prevents pigment accumulation in the stratum corneum; at the same time, it activates the proliferation of skin fibroblasts, promotes collagen synthesis, and improves the smoothness of the skin in acne scar areas, especially having a repairing effect on pitted acne scars; in addition, keratinase can also increase the skin penetration rate of dragon's blood extract and niacinamide, enhancing the utilization rate of the ingredients.

[0018] Niacinamide: It inhibits the transfer of melanin from melanocytes to keratinocytes, reducing pigmentation at the source; at the same time, it promotes the metabolism of the stratum corneum, accelerates the shedding of melanin-containing keratinocytes, and works synergistically with keratinase to improve the efficiency of keratin metabolism; in addition, niacinamide can repair the skin barrier, enhance the skin's resistance, and reduce sensitivity and redness caused by barrier damage in acne scar areas.

[0019] The three components work synergistically to form a closed-loop mechanism for fading blemishes: anti-inflammatory repair, keratin metabolism, and pigmentation inhibition. Compared to a single ingredient, this method provides a more comprehensive and efficient blemish-fading effect while also addressing skin repair and barrier protection. Detailed Implementation

[0020] The following is a further explanation with reference to specific implementation methods: Example 1

[0021] The preparation method of dragon's blood extract includes the following steps: 1. Raw material pretreatment: Take 50g of dragon's blood medicinal material, crush it and pass it through a 100-mesh sieve to obtain dragon's blood powder. 2. Preparation of low cosolvent: Weigh 10g of betaine (hydrogen bond acceptor), 30g of 1,3-butanediol, and 20g of glycerol (hydrogen bond donor, 1,3-butanediol to glycerol mass ratio 3:2), mix and stir at 45℃ for 12 minutes to form a transparent low cosolvent system. 3. Ultrasonic extraction: Mix the dragon's blood powder with a low co-solvent at a material-to-liquid ratio of 1:15 (g / mL), place it in an ultrasonic device, and set the ultrasonic power to 250W, the extraction temperature to 40℃, and the extraction time to 45 minutes. 4. Post-processing: The extract was centrifuged at 9000 r / min for 12 minutes, and the supernatant was filtered through a 0.45 μm filter membrane to obtain the dragon's blood extract.

[0022] Examples 2-4 The formulations for Examples 2-4 are shown in Table 1.

[0023] Table 1. Formula of Fading Scar Repair Essence

[0024] The preparation methods in Examples 2-4 include the following steps: 1. Add phase A raw material to water in sequence, heat to 80-85℃, stir until completely dissolved and homogeneous, and cool to below 40℃.

[0025] 2. Add phase B raw material to phase A and stir evenly.

[0026] 3. When the system temperature drops below 35℃, add phase C keratinase and stir gently to avoid enzyme inactivation.

[0027] 4. Finally, add phase D pH adjuster to adjust the final pH value of the product to 6.5.

[0028] 5. Stir well and discharge to obtain an essence with blemish-repairing effects.

[0029] Comparative Example 1: Traditional alcohol-extracted dragon's blood extract Take 5g of dragon's blood powder, add 75mL of 70% ethanol aqueous solution, place it in an ultrasonic device, set the ultrasonic power to 250W, the extraction temperature to 40℃, and the extraction time to 45 minutes, and centrifuge to collect the supernatant.

[0030] Comparative Example 2 The difference from Example 2 is that the dragon's blood extract used is the dragon's blood extract obtained by the conventional alcohol extraction method in Comparative Example 1.

[0031] Comparative Example 3 The difference from Example 2 is that it does not contain dragon's blood extract.

[0032] Comparative Example 4 The difference from Example 2 is that it does not contain nicotinamide.

[0033] Comparative Example 5 The difference from Example 2 is that it does not contain keratinase.

[0034] Effect test: Determination of the inhibition rate of tyrosinase activity from dragon's blood extract 1. The tyrosinase inhibition rate (%) of the dragon's blood extracts prepared in Example 1 and Comparative Example 1 was determined according to T / GDCA 006—2021 "Test Method for Inhibition of Tyrosinase Activity by Cosmetic Raw Materials (In Vitro Method)".

[0035] Test sample: 1g of dragon's blood extract and 99g of deionized water, mixed evenly.

[0036] 2. The hyaluronidase inhibition rate (%) of the dragon's blood extracts prepared in Example 1 and Comparative Example 1 was determined according to T / JSRH 003-2023 "Test of anti-allergic activity of cosmetic raw materials - hyaluronidase inhibition method".

[0037] Test sample: 1g of dragon's blood extract and 99g of deionized water, mixed evenly.

[0038] The experimental data for Experiments 1-2 are detailed in Table 2.

[0039] Table 2:

[0040] 3. Evaluation of human efficacy Eighty participants aged 18-60 were randomly divided into eight groups of ten each: Examples 2-4, Comparative Examples 2-5, and a blank control group. These were Asian adult test subjects with significant acne scars. Under normal circumstances, the volunteers continuously used the test samples on their entire face for 28 days to evaluate the efficacy of the test samples prepared from the acne-skin fading composition of each example and comparative example.

[0041] Blank control: 5g glycerin, 0.3g hydroxyethyl cellulose, 4g 1,3-butanediol, 0.5g fentanyl, 0.5g hexanediol, appropriate amount of pH adjuster (citric acid / sodium citrate) to pH 6.5, and 100g deionized water, mix well.

[0042] The specific steps are as follows: Volunteers applied 5ml of the test sample to their face after cleansing each morning and evening, covering all acne scars and massaging until absorbed. Follow-up visits were conducted on days 7, 14, and 28, with 10 volunteers per group. On the day of the visit, volunteers washed their faces with water without applying any product and sat quietly for 30 minutes in an air-conditioned room with a temperature of 23±1℃ and humidity of 50±5%. Instruments were used to quantify and photograph the improvement of different types of acne scars, including measuring the a* value with VISIA7 and measuring the EI value (heme content) and MI value (melanin content) with MX18. Changes in a* and EI values ​​represent the degree of improvement of red acne scars; lower values ​​indicate more significant fading. Changes in MI values ​​represent the degree of improvement of black acne scars; lower values ​​indicate more significant fading. The transepidermal water loss (TEWL) value of the subjects' skin was measured using the TM Hex probe, and the average value for each group of 10 was calculated, further determining the transepidermal water loss rate.

[0043] The improvement of acne scars is represented by the improvement rate of each value, calculated as follows: .

[0044] TWEL value change rate = [(Average transdermal water loss of 10 people in each group after using the test sample - Average transdermal water loss of 10 people in each group before using the test sample (T0)) / Average transdermal water loss of 10 people in each group before using the test sample (T0)] × 100% The results of the light-marking effect are shown in Table 3.

[0045] Table 3. Effects of Light Printing

[0046] The results of the repair efficacy are shown in Table 4.

[0047] Table 4 Repair Efficacy

[0048] 4. Patch Test Test substances: Examples 2-4 and Comparative Examples 2-5 of the present invention; Participants: A total of 34 people, including 8 men and 26 women, aged 20-40 years, who met the criteria for voluntary participation. Test method: A closed test method was used. The test product was placed in a suitable patch applicator and applied to the flexor side of the subject's arm with hypoallergenic adhesive tape. The patch was then gently pressed with the palm of the hand to ensure even application to the skin for 24 hours. The blank control group consisted of subjects who did not use the product. Results of observation: The test apparatus was removed after 24 hours, and skin reactions were observed and recorded at 0.5h, 24h, and 48h. The severity of adverse skin reactions is shown in Table 5, and the test results are shown in Table 6.

[0049] Table 5. Grading Criteria for Skin Reactions in Occlusive Patch Tests

[0050] Table 6 Results of human skin patch test

[0051] Patch tests have shown that the essence obtained by this invention is non-irritating to human skin and will not cause allergies.

[0052] The embodiments and descriptions above are merely illustrative of the principles and preferred embodiments of the present invention. Various changes and modifications may be made to the present invention without departing from its spirit and scope, and all such changes and modifications fall within the scope of the present invention as claimed.

Claims

1. A composition for repairing fading marks, characterized in that, By weight percentage, it includes the following active ingredients: Dragon's Blood Extract 0.1%-5%, Keratinase 0.01%-0.5%, and Nicotinamide 1%-5%.

2. The fading mark repair composition according to claim 1, characterized in that: By weight percentage, it includes the following active ingredients: Dragon's Blood Extract 0.5%-3%, Keratinase 0.05%-0.3%, and Nicotinamide 2%-4%.

3. The fading mark repair composition according to claim 2, characterized in that: The dragon's blood extract was obtained by ultrasound-assisted eutectic solvent extraction, wherein the eutectic solvent is composed of hydrogen bond acceptors and hydrogen bond donors, wherein the hydrogen bond acceptor is betaine and the hydrogen bond donor is a mixture of 1,3-butanediol and glycerol.

4. The fading blemish repair composition according to claim 3, characterized in that: In the eutectic solvent, the mass ratio of betaine, 1,3-butanediol and glycerol is 1:(2-4):(1-3).

5. The fading blemish repair composition according to any one of claims 1-4, characterized in that: It also contains cosmetically acceptable carriers and auxiliary ingredients.

6. The method for preparing the fading blemish repair composition according to any one of claims 4, characterized in that, Includes the following steps: Preparation of eutectic solvent: Betaine, 1,3-butanediol and glycerol are mixed in proportion and stirred at 60-80℃ until a homogeneous and transparent liquid is formed to obtain the eutectic solvent; Ultrasonic-assisted extraction: Mix the raw material powder of dragon's blood with the above-mentioned eutectic solvent at a material-to-liquid ratio of 1:10-1:30 (g / mL), place it in an ultrasonic device, and ultrasonically extract at 40-60℃ for 20-60 minutes; Separation and post-processing: Centrifuge or filter the extract and take the supernatant to obtain the dragon's blood extract.

7. The method for preparing the fading blemish repair composition according to claim 6, characterized in that: The mass ratio of 1,3-butanediol to glycerol is 3:

2.

8. The method for preparing the fading blemish repair composition according to claim 6, characterized in that: The ultrasonic device is set with an ultrasonic power of 250W.

9. The method for preparing the fading blemish repair composition according to claim 6, characterized in that: The centrifugation speed was 9000 r / min and the centrifugation time was 12 min.

10. The use of the acne scar fading and repairing composition as described in claim 5 in the preparation of cosmetics for fading acne scars, improving post-inflammatory hyperpigmentation, and repairing the skin barrier, wherein the dosage form is one of serum, toner, lotion, gel, or mask.

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