Multiplexed nonlinear optical signal collection device

By using a multi-channel nonlinear optical signal collection device, the problems of low efficiency and insufficient sensitivity in multi-angle detection of existing equipment are solved, realizing multi-angle synchronous measurement and efficient signal capture, which is suitable for the accurate detection of a variety of nonlinear optical signals.

CN121877809BActive Publication Date: 2026-06-02CHANGCHUN INST OF OPTICS FINE MECHANICS & PHYSICS CHINESE ACAD OF SCI

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
CHANGCHUN INST OF OPTICS FINE MECHANICS & PHYSICS CHINESE ACAD OF SCI
Filing Date
2026-03-17
Publication Date
2026-06-02

AI Technical Summary

Technical Problem

Existing nonlinear optical signal collection devices have low multi-angle detection efficiency and insufficient sensitivity, making it difficult to simultaneously capture the subtle features of multiple nonlinear optical signals.

Method used

A multi-channel nonlinear optical signal collection device was designed, including a laser emission module, an adjustment stage, a reflection transmission unit, a transmission transmission unit, a flip mirror, a first signal processing module, and a second signal processing module. The sample pose is adjusted by the adjustment stage, and multi-angle signal collection and processing are achieved by using the flip mirror and various optical components. The device is then combined with a spectrometer and a photomultiplier tube for full-band analysis.

Benefits of technology

It enables simultaneous measurement of multi-angle nonlinear optical signals, improving detection efficiency and sensitivity. It can accurately capture subtle features of various nonlinear optical signals and is suitable for signal characterization of different samples.

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Abstract

The present application belongs to the technical field of optical signal collection imaging, and particularly relates to a multi-path nonlinear optical signal collection device. An adjusting table is used to carry a sample to be measured and adjust the spatial pose of the sample to be measured. A laser emission module is used to emit laser. After the sample to be measured is excited by the laser, nonlinear optical signals are obtained. The nonlinear optical signals are incident on a flip mirror through a reflection transmission unit or a transmission transmission unit. The flip mirror reflects the nonlinear optical signals to a first signal processing module or a second signal processing module through angle adjustment. The first signal processing module processes the nonlinear optical signals from the reflection transmission unit or the transmission transmission unit to obtain spectral analysis processing results. The second signal processing module processes the nonlinear optical signals from the reflection transmission unit or the transmission transmission unit to obtain electrical signal processing results. The present application is used to collect nonlinear optical signals of reflection, transmission and oblique incidence and other far-end detection optical paths.
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Description

Technical Field

[0001] This invention belongs to the field of optical signal collection and imaging technology, and particularly relates to a multi-channel nonlinear optical signal collection device. Background Technology

[0002] Existing nonlinear optical signal collection devices, when adapting to optical signals with different excitation or collection angles, are mostly limited by the inherent constraints of the shaping optical path, and can only be used for signal acquisition at a single excitation or collection angle. Meanwhile, the collection devices need to handle the collection of multiple different types of nonlinear optical signals, which places high demands on their optical response range. In summary, current work on the detection of multi-angle nonlinear optical signals still faces the following prominent problems:

[0003] Firstly, the existing equipment has a relatively limited measurement angle, which makes it impossible to complete signal measurement at different angles in a short time, resulting in low detection efficiency and difficulty in meeting the needs of multi-angle synchronous detection.

[0004] Secondly, the coexistence of multiple nonlinear optical signals during the detection process places stringent demands on the performance of the detection device, while the detection sensitivity of existing equipment is insufficient, making it difficult to accurately capture the subtle features of various signals.

[0005] The two aforementioned shortcomings mean that while current nonlinear optical signal detection devices can meet the basic requirements of nonlinear optical signal research to a certain extent, they still have obvious drawbacks and limitations. In particular, there is still considerable room for improvement in detecting various nonlinear components of different samples and characterizing signals of nonlinear samples with different morphologies. Summary of the Invention

[0006] In view of this, the present invention aims to provide a multi-path nonlinear optical signal collection device to solve the problems of low detection efficiency, difficulty in meeting the needs of multi-angle synchronous detection and insufficient detection sensitivity of existing detection equipment. The present invention is used to collect nonlinear optical signals from remote detection optical paths such as reflection, transmission and oblique incidence, and has stronger adaptability and adjustability.

[0007] To achieve the above objectives, the technical solution created by this invention is implemented as follows:

[0008] A multi-channel nonlinear optical signal collection device includes: a laser emitting module, an adjustment stage, a reflection transmission unit, a transmission transmission unit, a flip mirror, a first signal processing module, and a second signal processing module, wherein:

[0009] The adjustment stage is used to hold the sample to be tested and adjust its spatial orientation. The laser emission module is used to emit laser light. After the sample to be tested is excited by the laser, it obtains a nonlinear optical signal. The nonlinear optical signal is incident on the flip mirror through the reflection transmission unit or the transmission transmission unit. The flip mirror reflects the nonlinear optical signal to the first signal processing module or the second signal processing module by adjusting the angle. The first signal processing module processes the nonlinear optical signal from the reflection transmission unit or the transmission transmission unit to obtain the spectral analysis processing result. The second signal processing module processes the nonlinear optical signal from the reflection transmission unit or the transmission transmission unit to obtain the electrical signal processing result.

[0010] Furthermore, the laser emission module includes a laser, a polarization modulation module, a first beam splitter, a first objective lens, and a light source. The laser emitted by the laser is modulated into polarized light with a preset polarization state by the polarization modulation module. At the same time, the light source emits white light, which is used to supplement the measurement area of ​​the sample under test. After the polarized light is transmitted through the first beam splitter, transmitted light is obtained. After the white light is reflected by the first beam splitter, reflected light is obtained. The transmitted light and reflected light are focused by the first objective lens and then incident on the surface of the sample under test.

[0011] Furthermore, if the preset polarization state is s-polarization or p-polarization, the polarization modulation module is a first Glan prism; if the preset polarization state is circularly polarized light, the polarization modulation module includes a first Glan prism and a first quarter-wave plate arranged sequentially in the direction of light propagation.

[0012] Furthermore, the reflection transmission unit includes a second objective lens, a second beam splitter, an imaging device, a filter, a reflector, a filter module, and a second Glan prism, wherein:

[0013] The nonlinear optical signal reflected by the sample under test is incident on the second beam splitter through the second objective lens. Part of the signal is incident on the imaging device, which then images the measurement area of ​​the excited sample under test. The other part of the signal is incident on the flip mirror through the filter, the mirror, the filter module and the second Glan prism in sequence.

[0014] The transmission unit includes a third objective lens and a third Glan prism. The nonlinear optical signal transmitted by the sample under test is irradiated onto the flip mirror through the third objective lens and the third Glan prism.

[0015] Furthermore, the imaging device performs image acquisition every preset time interval. Based on the results of two adjacent image acquisitions, it determines whether the sample to be tested and the laser spot on the sample to be tested have moved. If so, the spatial pose of the sample to be tested is adjusted by the adjustment stage so that the spatial position of the sample to be tested and the position of the laser spot on the sample to be tested remain unchanged.

[0016] Furthermore, the filter module is a pinhole, which is used to control the imaging depth of the sample under test.

[0017] Furthermore, the filtering module includes a filter group and a coupling lens arranged sequentially along the optical path propagation direction. The filter group is used to filter signal light of a preset wavelength, and the coupling lens is used to couple the signal light to the optical fiber so that the signal light illuminates the second Glan prism.

[0018] Furthermore, nonlinear optical signals include two-photon fluorescence signals and second harmonic signals.

[0019] Furthermore, the first signal processing module includes a full-band spectrometer or multiple spectrometers with different detection bands, and the multiple spectrometers with different detection bands work together to achieve full-band spectral analysis of nonlinear optical signals.

[0020] Furthermore, the second signal processing module includes a full-band photomultiplier tube or multiple photomultiplier tubes with different detection spectral bands, and the multiple photomultiplier tubes with different detection spectral bands work together to achieve photoelectric conversion of nonlinear optical signals.

[0021] Compared with the prior art, the present invention can achieve the following beneficial effects:

[0022] This invention creates a multi-channel nonlinear optical signal collection device, which effectively solves the technical pain points of existing equipment, such as single measurement angle and insufficient detection sensitivity, and has significant practical value and technical advantages. This invention can simultaneously collect two-photon fluorescence signals and second harmonic signals in front of a remote detection device and converge them into a dual-channel nonlinear optical signal collection optical path (i.e., the nonlinear optical signal is incident on a flip mirror via a reflection transmission unit or a transmission transmission unit; the flip mirror, through angle adjustment, reflects the nonlinear optical signal to a first signal processing module or a second signal processing module), greatly improving the collection efficiency of nonlinear optical signals and ensuring that most signals can be captured, providing more accurate data support for micron-level sample structure imaging.

[0023] Furthermore, the device of this invention overcomes the limitations of the shaped optical path and can be adapted to various remote detection optical paths such as reflection, transmission, and oblique incidence, enabling simultaneous measurement of multi-angle nonlinear optical signals without frequent optical path adjustments. This significantly shortens measurement time, improves detection efficiency, and solves the problem that existing equipment cannot complete multi-angle signal measurement in a short time. Simultaneously, the combination of the spectrometer and photomultiplier tube effectively enhances detection sensitivity, accurately capturing the subtle features of multiple coexisting nonlinear optical signals, meeting the signal characterization needs of nonlinear components and nonlinear samples with different morphologies. Attached Figure Description

[0024] The accompanying drawings, which form part of this invention, are used to provide a further understanding of the invention. The illustrative embodiments and descriptions of the invention are used to explain the invention and do not constitute an undue limitation of the invention. In the drawings:

[0025] Figure 1 A schematic diagram of the structure of the multi-channel nonlinear optical signal collection device described in the embodiment of the present invention;

[0026] Figure 2 The diagram shows the structure of the reflection transmission optical path and the transmission optical path as described in the embodiments of the present invention.

[0027] Explanation of reference numerals in the attached figures:

[0028] 1. Laser; 2. Polarization modulation module; 3. Light source; 4. First beam splitter; 5. First objective lens; 6. Second objective lens; 7. Imaging device; 8. Second beam splitter; 9. Filter; 10. Reflector; 11. Filter module; 12. Second Glan prism; 13. Adjustment stage; 14. Third objective lens; 15. Third Glan prism; 16. Flip mirror; 17. First signal processing module; 18. Second signal processing module; 19. Filter unit; 20. Coupler; 11-1. Filter group; 11-2. Coupler lens. Detailed Implementation

[0029] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to the accompanying drawings and specific embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and do not constitute a limitation thereof.

[0030] It should be noted that, unless otherwise specified, the embodiments and features described in the present invention can be combined with each other.

[0031] In the description of this invention, it should be understood that the terms "center," "longitudinal," "lateral," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," and "outer," etc., indicating orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation on this invention. Furthermore, the terms "first," "second," etc., are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Thus, features defined with "first," "second," etc., may explicitly or implicitly include one or more of that feature. In the description of this invention, unless otherwise stated, "a plurality of" means two or more.

[0032] In the description of this invention, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art will understand the specific meaning of the above terms in this invention based on the specific circumstances.

[0033] The invention will now be described in detail with reference to the accompanying drawings and embodiments.

[0034] like Figures 1-2 As shown, this invention proposes a multi-channel nonlinear optical signal collection device, comprising: a laser emission module, an adjustment stage 13, a reflection transmission unit, a transmission transmission unit, a flip mirror 16, a first signal processing module 17, and a second signal processing module 18, wherein:

[0035] The adjustment stage 13 is used to carry the sample to be tested and adjust the spatial pose of the sample. The laser emission module is used to emit laser. After the sample is excited by the laser, it obtains a nonlinear optical signal. The nonlinear optical signal is incident on the flip mirror 16 through the reflection transmission unit or the transmission transmission unit. The flip mirror 16 reflects the nonlinear optical signal to the first signal processing module 17 or the second signal processing module 18 by adjusting the angle. The first signal processing module 17 processes the nonlinear optical signal from the reflection transmission unit or the transmission transmission unit to obtain the spectral analysis processing result. The second signal processing module 18 processes the nonlinear optical signal from the reflection transmission unit or the transmission transmission unit to obtain the electrical signal processing result.

[0036] It should be noted that the adjustment stage 13 of this invention is used to support the sample to be tested and flexibly adjust its spatial orientation to provide a suitable angle for laser excitation. The laser emitting module emits a laser to excite the sample to generate a nonlinear optical signal, which is transmitted to the flip mirror 16 via a reflection transmission unit or a transmission transmission unit. The flip mirror 16 can selectively reflect the nonlinear optical signal to the first signal processing module 17 or the second signal processing module 18 by adjusting its angle. The first signal processing module 17 processes the received signal and outputs the spectral analysis result; the second signal processing module 18 processes the signal to obtain the electrical signal processing result. This invention has a simple structure and flexible adjustment, and can efficiently realize the transmission, switching, and differentiated processing of nonlinear optical signals to meet diverse signal analysis needs.

[0037] In some embodiments, the laser emitting module includes a laser 1, a polarization modulation module 2, a first beam splitter 4, a first objective lens 5, and a light source 3. The laser emitted from the laser 1 is modulated into polarized light with a preset polarization state by the polarization modulation module 2. At the same time, the light source 3 emits white light, which is used to supplement the measurement area of ​​the sample to be tested. After the polarized light is transmitted through the first beam splitter 4, transmitted light is obtained. After the white light is reflected by the first beam splitter 4, reflected light is obtained. The transmitted light and the reflected light are focused by the first objective lens 5 and incident on the surface of the sample to be tested.

[0038] It should be noted that laser 1 is a femtosecond pulsed laser, and the emitted laser is a pulsed laser. Femtosecond pulsed lasers have ultra-short pulse widths (femtosecond level) and high peak power, which can effectively excite the sample under test to generate nonlinear optical signals such as two-photon fluorescence and second harmonics.

[0039] In some embodiments, if the preset polarization state is s-polarization or p-polarization, then the polarization modulation module 2 is a first Glan prism; if the preset polarization state is circularly polarized light, then the polarization modulation module 2 includes a first Glan prism and a first quarter-wave plate arranged sequentially in the direction of light propagation.

[0040] It should be noted that the laser generated by laser 1 is linearly polarized or randomly polarized. Therefore, this invention first converts the laser into linearly polarized light with a specific polarization direction using a Glan prism (full name: Glan-Thompson prism). If circularly polarized light is required, the linearly polarized light generated by the Glan-Thompson prism is then passed through a 1 / 4 wavelength liquid crystal glass plate (i.e., a 1 / 4 wave plate). The liquid crystal glass plate can generate a 1 / 4 wavelength phase difference along its fast and slow axes, and the difference between different wavelengths can be compensated for by voltage to ensure phase difference stability.

[0041] In some embodiments, the reflection transmission unit includes a second objective lens 6, a second beam splitter 8, an imaging device 7, a filter 9, a reflector 10, a filter module 11, and a second Glan prism 12, wherein:

[0042] The nonlinear optical signal reflected by the sample under test is incident on the second beam splitter 8 through the second objective lens 6. Part of the signal is incident on the imaging device 7, so that the imaging device 7 can image the measurement area of ​​the sample under test after excitation. The other part of the signal is incident on the flip mirror 16 through the filter 9, the reflector 10, the filter module 11, and the second Glan prism 12 in sequence.

[0043] The transmission unit includes a third objective lens 14 and a third Glan prism 15. The nonlinear optical signal transmitted by the sample under test is irradiated onto the flip mirror 16 through the third objective lens 14 and the third Glan prism 15.

[0044] It should be noted that the white light emitted by the light source 3 is used to illuminate the measurement area of ​​the sample to be tested, so that the imaging device 7 can acquire the image of the measurement area of ​​the sample after excitation. The imaging device 7 can be turned on or off according to actual needs. In addition, the filter 9 is used to filter out the excitation laser, that is, the laser emitted by the laser emitting module, to avoid the excitation laser from generating thermal noise in the subsequent detection process.

[0045] It should also be noted that between the third objective lens 14 and the third Glan prism 15, there is also a filter unit 19 and a coupling mirror 20 arranged sequentially in the direction of light propagation. The filter unit 19 includes a filter 9.

[0046] In some embodiments, the imaging device 7 performs image acquisition once every preset time interval. Based on the results of two adjacent image acquisitions, it determines whether the sample to be tested and the laser spot on the sample to be tested have moved. If so, the spatial pose of the sample to be tested is adjusted by the adjustment stage 13 so that the spatial position of the sample to be tested and the position of the laser spot on the sample to be tested remain unchanged.

[0047] It should be noted that the preset time is one minute. The adjustment stage 13 has five degrees of freedom of motion adjustment function, specifically including horizontal X-axis translation, horizontal Y-axis translation, vertical Z-axis lifting, pitch adjustment around the horizontal axis, and circumferential rotation adjustment around the vertical axis. Through the coordinated operation of the above five dimensions, the adjustment stage 13 can accurately carry the sample to be tested and fully control its spatial position and attitude. It can achieve precise positioning of the sample measurement area in the horizontal plane and vertical focusing distance adaptation, and can flexibly adjust the tilt angle and axial orientation of the sample surface, thereby providing a stable and accurate positional guarantee for the laser to efficiently excite the sample at a preset angle.

[0048] In the adjustment stage 13, the support stage used to support the sample to be tested has a hollow structure. The sample is placed on the surface of the fully transparent sample carrier, and the sample carrier is placed on the support stage. The sample carrier is a rectangular sample carrier, and the length of the sample carrier is greater than the maximum length of the hollow part of the support stage. The two ends of the sample carrier are supported on the edge of the hollow part, and the whole sample spans the hollow part of the support stage. The area of ​​the sample to be tested is smaller than the area of ​​the hollow part, and the entire sample to be tested is located in the area corresponding to the hollow part, so that the sample to be tested is not obstructed. The transmission light path is located directly below the hollow part and is used to receive light transmitted from various angles of the sample to be tested. In addition, it should be noted that the test area of ​​the sample to be tested is located on the sample carrier.

[0049] Furthermore, the filter module 11 is a small hole, which is used to control the imaging depth of the sample under test.

[0050] It should be noted that when the user does not need to select the wavelength of the optical signal after filtering the optical module 11, the filter module 11 is configured as a pinhole. The pinhole can also limit the light beam aperture and filter out stray light. However, if there are special requirements for the wavelength of the optical signal after filtering the optical module 11, the filter module 11 is configured to include a filter group 11-1 and a coupling lens 11-2 arranged sequentially along the optical path propagation direction. In this case, the wavelength of the optical signal after filtering the optical module 11 is set by setting the filter group 11-1.

[0051] Furthermore, the filtering module 11 includes a filter group 11-1 and a coupling lens 11-2 arranged sequentially along the optical path propagation direction. The filter group 11-1 is used to filter signal light of a preset wavelength, and the coupling lens 11-2 is used to couple the signal light to the optical fiber so that the signal light illuminates the second Glan prism 12.

[0052] Furthermore, nonlinear optical signals include two-photon fluorescence signals and second harmonic signals.

[0053] Furthermore, the first signal processing module 17 includes a full-band spectrometer or multiple spectrometers with different detection bands, and the multiple spectrometers with different detection bands work together to achieve full-band spectral analysis of nonlinear optical signals.

[0054] It should be noted that, for example, if the nonlinear optical signal generated by the sample under test after laser excitation covers the entire 200nm-1500nm band, a spectrometer covering this entire band can be selected to directly perform full-band scanning analysis of the signal and obtain complete spectral characteristics. Alternatively, three spectrometers with different detection bands can be used in concert. The first spectrometer detects the visible light band signal of 200nm-900nm, the second detects the near-infrared band signal of 400nm-1000nm, and the third detects the mid-far-infrared band signal of 900nm-1500nm. The three spectrometers are synchronously acquired and data fused to jointly achieve accurate spectral analysis of the nonlinear optical signal of the entire 200nm-1500nm band, adapting to the fine detection needs of different spectral band signals.

[0055] Furthermore, the second signal processing module 18 includes a full-band photomultiplier tube or multiple photomultiplier tubes with different detection spectral bands, and the multiple photomultiplier tubes with different detection spectral bands work together to achieve photoelectric conversion of nonlinear optical signals.

[0056] It should be noted that, for example, if it is necessary to perform photoelectric conversion on the nonlinear optical signal generated by the sample under test across the entire wavelength range, a photomultiplier tube covering the entire wavelength range of the signal can be selected to directly convert the received nonlinear optical signal (such as two-photon fluorescence, second harmonic signal) into an electrical signal and transmit it to the subsequent processing unit. Alternatively, according to the signal spectral distribution, two photomultiplier tubes with different detection spectral ranges can be selected to work together. One of them is adapted to two-photon fluorescence signals of 200nm-900nm, which is specifically used to convert optical signals in this wavelength range into corresponding electrical signals. The other is adapted to second harmonic signals of 800nm-1500nm, which is responsible for the conversion of optical signals in this wavelength range into electrical signals. The two work together to ensure that nonlinear optical signals in different spectral ranges can be photoelectrically converted efficiently and accurately, ensuring the accuracy of the subsequent electrical signal processing results.

[0057] It should be understood that the various forms of processes shown above can be used to reorder, add, or delete steps. For example, the steps described in this invention disclosure can be executed in parallel, sequentially, or in different orders, as long as the desired result of the technical solution disclosed in this invention can be achieved, and this is not limited herein.

[0058] The specific embodiments described above do not constitute a limitation on the scope of protection of this invention. Those skilled in the art should understand that various modifications, combinations, sub-combinations, and substitutions can be made according to design requirements and other factors. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of this invention should be included within the scope of protection of this invention.

Claims

1. A multipass nonlinear optical signal collection device, comprising: include: The system comprises a laser emitting module, an adjustment stage, a reflection transmission unit, a transmission transmission unit, a flip mirror, a first signal processing module, and a second signal processing module, wherein: The adjustment stage is used to hold the sample to be tested and adjust its spatial orientation. The laser emission module is used to emit laser light. After the sample to be tested is excited by the laser, it obtains a nonlinear optical signal. The nonlinear optical signal is incident on the flip mirror through the reflection transmission unit or the transmission transmission unit. The flip mirror reflects the nonlinear optical signal to the first signal processing module or the second signal processing module by adjusting the angle. The first signal processing module processes the nonlinear optical signal from the reflection transmission unit or the transmission transmission unit to obtain the spectral analysis processing result. The second signal processing module processes the nonlinear optical signal from the reflection transmission unit or the transmission transmission unit to obtain the electrical signal processing result.

2. The multi-channel nonlinear optical signal collection device according to claim 1, characterized in that: The laser emission module includes a laser, a polarization modulation module, a first beam splitter, a first objective lens, and a light source. The laser emitted by the laser is modulated into polarized light with a preset polarization state by the polarization modulation module. At the same time, the light source emits white light, which is used to supplement the measurement area of ​​the sample under test. After the polarized light is transmitted through the first beam splitter, transmitted light is obtained. After the white light is reflected by the first beam splitter, reflected light is obtained. The transmitted light and reflected light are focused by the first objective lens and then incident on the surface of the sample under test.

3. The multi-channel nonlinear optical signal collection device according to claim 1, characterized in that: If the preset polarization state is s-polarization or p-polarization, the polarization modulation module is a first Glan prism; if the preset polarization state is circularly polarized light, the polarization modulation module includes a first Glan prism and a first quarter-wave plate arranged sequentially in the direction of light propagation.

4. The multi-channel nonlinear optical signal collection device according to claim 2, characterized in that: The reflection transmission unit includes a second objective lens, a second beam splitter, an imaging device, a filter, a reflector, a filter module, and a second Glan prism, wherein: The nonlinear optical signal reflected by the sample under test is incident on the second beam splitter through the second objective lens. Part of the signal is incident on the imaging device, which then images the measurement area of ​​the excited sample under test. The other part of the signal is incident on the flip mirror through the filter, the mirror, the filter module and the second Glan prism in sequence. The transmission unit includes a third objective lens and a third Glan prism. The nonlinear optical signal transmitted by the sample under test is irradiated onto the flip mirror through the third objective lens and the third Glan prism.

5. The multi-channel nonlinear optical signal collection device according to claim 4, characterized in that: The imaging device acquires an image every preset time interval. Based on the results of two consecutive image acquisitions, it determines whether the sample under test and the laser spot on the sample under test have moved. If so, it adjusts the spatial pose of the sample under test through the adjustment stage so that the spatial position of the sample under test and the position of the laser spot on the sample under test remain unchanged.

6. The multi-channel nonlinear optical signal collection device according to claim 4, characterized in that: The filter module is a small aperture, which is used to control the imaging depth of the sample under test.

7. The multi-channel nonlinear optical signal collection device according to claim 4, characterized in that: The filtering module includes a filter group and a coupling lens arranged sequentially along the optical path propagation direction. The filter group is used to filter signal light of a preset wavelength, and the coupling lens is used to couple the signal light to the optical fiber so that the signal light illuminates the second Glan prism.

8. The multi-channel nonlinear optical signal collection device according to claim 1, characterized in that: Nonlinear optical signals include two-photon fluorescence signals and second harmonic signals.

9. The multi-channel nonlinear optical signal collection device according to claim 1, characterized in that: The first signal processing module includes a full-band spectrometer or multiple spectrometers with different detection bands. Multiple spectrometers with different detection bands work together to achieve full-band spectral analysis of nonlinear optical signals.

10. The multi-channel nonlinear optical signal collection device according to claim 1, characterized in that: The second signal processing module includes a full-band photomultiplier tube or multiple photomultiplier tubes with different detection spectral bands. Multiple photomultiplier tubes with different detection spectral bands work together to achieve photoelectric conversion of nonlinear optical signals.