Immune quantitative detection device for blood tumor markers

Abstract

The invention discloses an immune quantitative detection device for hematologic tumor markers in the technical field of immune quantitative detection.The immune quantitative detection device comprises a shell, an immune reaction module and a detection module are arranged in the shell, the immune reaction module comprises a sample containing container, a driving piece is installed in the sample containing container and connected with a cylindrical cam, and the cylindrical cam is connected with the detection module; a sliding groove is formed in the outer side wall of the cylindrical cam, a sliding block is arranged in the sliding groove in a sliding fit mode and connected with a vertical rod, the vertical rod is sleeved with a spring, and the spring is sleeved with a drainage tube. The vertical rod is of a hollow structure, round holes communicated with the outside are formed in the middle and the bottom end of the vertical rod, the drainage tube penetrates through the bottom of the sample containing container and is fixedly connected with the sample containing container, the detection module comprises a detector, the detector is in signal connection with a controller, and the controller is in signal connection with the motor. The detection process is automatically completed by the detection device and can be completed in a short time, the detection efficiency is improved, the size is small, carrying is convenient, and detection work can be carried out anytime and anywhere.

Description

Technical Field

[0001] This invention belongs to the field of immunoquantitative detection technology, specifically an immunoquantitative detection device for blood tumor markers. Background Technology

[0002] People with weakened immune systems are more susceptible to cancer: the occurrence, development, recurrence rate, and survival time of cancer are all closely related to the body's immune status. The human immune system consists of immune organs, immune cells, immune molecules, and the lymphatic circulation network. Immune cells travel to all organs and tissues throughout the body via the bloodstream.

[0003] Quantitative analysis of changes in the proportion of T lymphocyte subsets in human peripheral blood and qualitative and quantitative analysis of changes in T cell antigen receptor (TCR) diversity are used to assess the cellular immune response capacity (including anti-tumor and anti-infection capacity) of human individuals, so as to guide the determination of clinical anti-tumor treatment plans.

[0004] Commonly used immunological detection techniques include: 1. Immunoturbidimetry, which quantitatively detects soluble antigens and antibodies by measuring the light refraction or absorption of the complexes formed. However, this method has low sensitivity and is not suitable for trace detection. 2. Solid-phase enzyme immunoassay is a highly sensitive detection method with significant advantages such as ease of operation, high sensitivity, wide linear response range, and ease of automation. However, its long reaction time limits its use. 3. Chemiluminescence immunoassay is a highly sensitive micro and trace analysis technique with significant advantages such as ease of operation, high sensitivity, wide linear response range, and ease of automation. However, its high requirements for detection equipment also affect its use. In addition, immunofluorescence labeling, flow cytometry, and colloidal gold technology are also commonly used detection techniques, but each has its corresponding shortcomings.

[0005] To address the above problems, this invention aims to provide a solution that is highly sensitive, suitable for trace detection, has a short reaction time, and requires minimal equipment. This design enables rapid, accurate, and reliable detection of blood tumor markers. Therefore, this invention proposes an immunoassay device for blood tumor markers to solve the aforementioned problems. Summary of the Invention

[0006] This invention proposes an immunoassay device for quantitative detection of blood tumor markers. The entire process, from mixing and incubating serum samples and antibodies to detection and result output, can be completed automatically by the device, improving detection efficiency. It can be completed in a short time, shortening the patient's waiting time. It is small in size, easy to carry, and can be used for testing anytime and anywhere.

[0007] To achieve the above objectives, the technical solution of the present invention is as follows: An immunoassay device for quantitative detection of blood tumor markers includes a housing, an immunoassay module and a detection module inside the housing, the immunoassay module includes a sample container, the sample container is fixedly connected to the upper inner wall of the housing, the housing and the sample container are provided with a liquid inlet at the top, a driving component is fixedly connected to the center of the inner top wall of the sample container, a cylindrical cam is coaxially fixedly connected to the output shaft of the driving component, a groove is provided on the outer wall of the cylindrical cam, the groove is a vertical groove structure that runs obliquely through, a slider is slidably fitted in the groove, a vertical rod is fixedly connected to the end of the slider away from the groove, a spring is sleeved on the vertical rod, and a drainage tube is sleeved on the spring.

[0008] The vertical rod is longer than the drainage tube. The vertical rod has a hollow structure and has round holes in the middle and bottom that communicate with the outside. The drainage tube passes through the bottom of the sample container and is fixedly connected to it. The detection module includes a detector, which is fixedly connected to the bottom wall inside the outer shell. The detector signal is connected to a controller, which is connected to a motor signal.

[0009] The above scheme achieves the following beneficial effects: the detection of tumor markers in serum samples is realized by using the immune response module and the detection module.

[0010] The entire testing process is automated. From mixing and incubating serum samples and antibodies to detection and result output, everything is completed automatically by the testing device, greatly improving testing efficiency. Using standards as a reference and employing a precise detector, the concentration of tumor markers in serum samples can be accurately measured, improving detection accuracy. The testing device uses highly sensitive antibodies that can detect low concentrations of tumor markers, which is beneficial for early diagnosis. The entire testing process can be completed in a short time, shortening patient waiting time and facilitating timely diagnosis and treatment. The testing device uses a reliable mechanical structure and detection method, ensuring the stability and reliability of the test results. If repeated testing is required, the same serum sample can be used for multiple trials to ensure the reliability of the results. The testing device is small and portable, allowing testing to be conducted anytime, anywhere.

[0011] While rotating, the cylindrical cam also stirs the mixture in the sample container, ensuring more thorough binding and improving the binding efficiency of tumor markers and antibodies in serum samples. The outer casing provides dust protection, safeguarding the immunoassay device for blood tumor markers and reducing contamination.

[0012] Furthermore, one side of the outer casing is made of transparent hard plastic, and a display screen is fixedly connected to the outer casing. The display screen is connected to the controller signal.

[0013] Beneficial effects: The transparent rigid plastic casing allows users to easily observe the internal workings of the testing device, making operation more convenient and ensuring that samples and reagents are added correctly. The transparent casing increases the transparency of the testing process, giving users greater confidence in the test results. Furthermore, users can view the device's status and test results on the display screen, making it easier to assess the device's operation and the reliability of the results.

[0014] Furthermore, the detection device connects to mobile devices via wireless communication technology to achieve remote monitoring and data transmission functions.

[0015] Beneficial effects: Wireless communication technology enables remote monitoring, allowing users to view the testing device's status and results in real time via mobile devices. This is extremely useful in scenarios requiring real-time results, such as when doctors conduct remote medical consultations at a patient's home, allowing them to view blood tumor marker test results via mobile devices for more accurate diagnoses. Wireless communication also enables data transmission, transferring data from the testing device to mobile devices or cloud storage. This allows users to access test results and data anytime, anywhere, facilitating analysis and processing. Furthermore, cloud storage enhances data security and reliability.

[0016] Furthermore, an opening for cleaning is provided on one side of the outer casing, a cover is installed at the opening, a latch is installed on the cover, and a locking pin that matches the latch is installed on the outer casing.

[0017] Beneficial effects: The opening on one side of the outer casing facilitates cleaning and maintenance of the testing device. The cover protects the opening, preventing dust and impurities from entering the device. A latch on the cover, along with matching locking pins on the outer casing, allows the cover to be locked when the device is not in use, enhancing security. The latch and locking pins fit snugly against the casing, increasing the device's stability and robustness. Furthermore, the cover design prevents moisture and dust from entering the device, improving its reliability.

[0018] Furthermore, a temperature control module is installed inside the casing. The temperature control module includes a temperature sensor, a heater, and a cooler, all of which are connected to the controller signal.

[0019] Beneficial Effects: The temperature control module ensures that the internal temperature of the detection device is maintained within a set range to support the accuracy of the immune response and the detection process. The heater brings samples and reagents to the appropriate temperature when needed, while the cooler lowers the temperature as required. The temperature control module prevents the internal temperature of the detection device from becoming too high or too low, thus protecting it from damage. Excessive heat may damage internal circuit boards or affect the normal operation of the detection device, while excessive cold may cause internal components to freeze or malfunction. Users can set appropriate temperature ranges as needed and connect the temperature control module to the controller. This makes it easier for users to operate the detection device and ensures the smooth progress of the detection process.

[0020] In summary, this design, which incorporates a temperature control module inside the casing of the immunoassay device for blood tumor markers, can improve the performance and accuracy of the device, while also protecting it, extending its lifespan, and facilitating user operation.

[0021] Furthermore, an alert module is embedded in the casing, which includes a buzzer and an indicator light, both of which are connected to the controller signal.

[0022] Beneficial effects: By using a buzzer and indicator lights in combination, users can more easily determine the status and results of the testing device. For example, the buzzer can emit different sounds or frequencies to indicate different states or results, while the indicator lights can provide more intuitive prompts by displaying different colors or flashing patterns. The reminder module can alert the user to the working status or results of the testing device by emitting sound or flashing lights. The design of the reminder module can avoid unnecessary operations by the user while waiting for test results, such as frequently checking the testing device screen or manually recording test results, thus reducing user steps and improving testing efficiency.

[0023] In summary, this design with an embedded reminder module on the outer casing can improve the efficiency and safety of the immunoassay device for blood tumor markers, while also making it easier for users to judge the status of the device and the test results.

[0024] Furthermore, a cover is detachably connected to the liquid inlet.

[0025] Beneficial effects: The cap protects the inlet from external contamination, ensuring the cleanliness and hygiene of the injected sample and reducing the entry of bacteria, dust, and other impurities into the sample container. The cap is easily removable, making the inlet easy to clean. Users can regularly clean and disinfect the inlet to maintain the cleanliness and hygiene of the detection device. This design, with a detachable cap at the inlet, is highly beneficial in immunoassay devices for blood tumor markers, improving the device's hygiene, safety, and stability, and facilitating user operation and maintenance.

[0026] Furthermore, an anti-slip layer is fixedly connected to the bottom wall of the outer shell.

[0027] Beneficial effects: The anti-slip layer increases friction between the testing device and the table or work surface, making the device more stable and less prone to sliding or tipping over. This is especially important for immunoassay devices for blood tumor markers, which require precise operation, improving stability and safety. The anti-slip layer also reduces friction and wear between the device and the table or work surface, thus extending the device's lifespan. Attached Figure Description

[0028] Figure 1 This is an isometric view of an embodiment of the immunoquantitative detection device for blood tumor markers of the present invention.

[0029] Figure 2 This is a cross-sectional view of an embodiment of the immunoquantitative detection device for blood tumor markers of the present invention.

[0030] Figure 3 This is a partial isometric view of the cylindrical cam in an embodiment of the immunoquantitative detection device for blood tumor markers of the present invention. Detailed Implementation

[0031] Embodiments of the present invention are described in detail below. Examples of these embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and should not be construed as limiting the present invention.

[0032] In the description of this invention, it should be understood that the terms "longitudinal", "lateral", "vertical", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", and "outer" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this invention.

[0033] In the description of this invention, unless otherwise specified and limited, it should be noted that the terms "installation", "connection" and "linking" should be interpreted broadly. For example, they can refer to mechanical or electrical connections, or internal connections between two components. They can be direct connections or indirect connections through an intermediate medium. Those skilled in the art can understand the specific meaning of the above terms according to the specific circumstances.

[0034] The following detailed description illustrates the specific implementation method:

[0035] The reference numerals in the accompanying drawings include: outer casing 1, liquid inlet 101, sample container 2, motor 201, cylindrical cam 3, slide 301, slider 4, vertical rod 5, spring 501, drainage tube 6, detector 7, and anti-slip layer 8.

[0036] Example 1, basically as shown in the attached document. Figure 1-3 As shown: An immunoassay device for quantitative detection of blood tumor markers includes a housing 1. An immunoassay module and a detection module are provided inside the housing 1. The immunoassay module includes a sample container 2, which is fixedly connected to the upper inner wall of the housing 1. An inlet 101 is provided at the top of the housing 1 and the sample container 2, and a cover is detachably connected to the inlet 101.

[0037] A driving component is fixedly connected to the center of the top wall of the sample container 2. In this embodiment, the driving component is a motor 201. A cylindrical cam 3 is coaxially fixedly connected to the output shaft of the driving component. The outer wall of the cylindrical cam 3 has a groove 301, which is a vertical groove structure that runs obliquely through the surface. A slider 4 slides within the groove 301. A vertical rod 5 is fixedly connected to the end of the slider 4 away from the groove 301. A spring 501 is sleeved on the vertical rod 5, and a drainage tube 6 is sleeved on the spring 501. The vertical rod 5 has a hollow structure, and round holes communicating with the outside are provided in the middle and at the bottom of the vertical rod 5.

[0038] The vertical rod 5 is longer than the drainage tube 6. The vertical rod 5 has a hollow structure and circular holes at its middle and bottom ends that communicate with the outside. The drainage tube 6 passes through the bottom of the sample container 2 and is fixedly connected to it. The detection module includes a detector 7, which is fixedly connected to the bottom wall inside the outer shell 1. The detector 7 is signal-connected to a controller, which is signal-connected to the motor 201. One side of the outer shell 1 is made of transparent rigid plastic, and a display screen is fixedly connected to it. The display screen is signal-connected to the controller. An anti-slip layer 8 is fixedly connected to the bottom wall of the outer shell 1.

[0039] The specific implementation process is as follows: Blood samples are collected from the patient using vacuum blood collection tubes or a syringe. The collected blood samples are placed in a centrifuge to separate the serum. The serum samples are then transferred to sample container 2 for immunoassay. A known concentration of standard is added to the serum sample as a reference. An appropriate concentration of antibody is added to each sample to allow it to specifically bind to the tumor marker. The sample is incubated for a period of time to allow the antibody to fully bind to the tumor marker. Detector 7 detects the concentration of the bound antibody. Based on the antibody concentrations of the standard and the unknown sample, the concentration of the tumor marker in the unknown sample is calculated.

[0040] In use, pour the serum sample and an appropriate concentration of antibody into the sample container 2 through the inlet 101, and close the cap at the inlet 101. Start the motor 201 via the controller. The motor 201 drives the cylindrical cam 3, causing the slider 4 to slide in the groove 301 on the surface of the cylindrical cam 3. The movement of the slider 4 drives the vertical rod 5, which moves up and down in the drainage tube 6. When the vertical rod 5 moves upward, the circular hole in the middle of the vertical rod 5 protrudes from the top of the drainage tube 6, and the serum sample and antibody mixture in the sample container 2 enters the interior of the vertical rod 5 through the circular hole. When the vertical rod 5 moves downward, the circular hole at the bottom of the vertical rod 5 protrudes from the bottom of the drainage tube 6, and the mixture inside the vertical rod 5 drips from the circular hole onto the detector 7. The controller then activates the detector 7, which detects the antibody concentration in the mixture. Based on the antibody concentrations of the standard and the unknown sample, the concentration of the tumor marker in the unknown sample is calculated. While rotating, the cylindrical cam 3 also stirs the mixture in the sample container 2, ensuring more thorough binding and improving the binding efficiency of tumor markers and antibodies in serum samples. The outer shell 1 provides a certain degree of dust protection, safeguarding the immunoassay device for blood tumor markers and reducing contamination.

[0041] The controller starts motor 201, which drives cylindrical cam 3 to rotate. This causes slider 4 to slide in groove 301 on the surface of cylindrical cam 3. The movement of slider 4 drives vertical rod 5, which moves up and down in drainage tube 6. This automated operation reduces manual operation and improves testing efficiency. The entire testing process can be completed in a short time, shortening patient waiting time and facilitating timely diagnosis and treatment. The testing device uses a reliable mechanical structure and testing method, ensuring the stability and reliability of the test results. If repeated testing is required, the same serum sample can be used for multiple tests to ensure the reliability of the results.

[0042] The detection device is small in size and easy to carry, allowing for testing anytime, anywhere. The entire device integrates multiple steps such as transfer, sample addition, incubation, and detection, which not only improves detection efficiency but also reduces the impact of the external environment on experimental results.

[0043] Example 2 differs from the above examples in that the detection device connects to a mobile device via wireless communication technology to achieve remote monitoring and data transmission functions.

[0044] The specific implementation process is as follows: In this embodiment, wireless communication technologies such as Wi-Fi or Bluetooth modules are used to communicate with mobile devices. Mobile devices include smartphones, tablets, and laptops. Users can receive data and status information from the detection device through their mobile devices. This data may include detection results, device status, and work progress. Simultaneously, users can also send commands to the detection device through their mobile devices, such as resetting the device and stopping operation.

[0045] During use, users can receive data and status information from the detection device via their mobile devices. This information can be displayed to users in the form of charts, tables, etc., so that users can better understand the device's operation and detection results. At the same time, users can also send commands to the detection device via their mobile devices to operate and control the device.

[0046] In summary, this design, which utilizes wireless communication technology to connect to mobile devices in the immunoquantitative detection device for blood tumor markers, improves the efficiency and convenience of the device, allowing users to remotely monitor and transmit data anytime, anywhere. It also reduces maintenance and time costs, and enhances the reliability and stability of the device.

[0047] Example 3 differs from the above examples in that: one side of the outer casing 1 has an opening for cleaning, a cover is installed at the opening, a latch is installed on the cover, and a locking pin that matches the latch is installed on the outer casing 1.

[0048] The specific implementation process is as follows: During use, the user can lock or unlock the cover by rotating the latch for cleaning and maintenance. When the testing device needs cleaning or maintenance, the user can open the cover and remove the internal components for cleaning or maintenance. After completion, the user can close and lock the cover to ensure that the internal components of the testing device will not accidentally slide out.

[0049] In summary, the design of this immunoassay device for blood tumor markers, which has an opening for cleaning on one side of the outer casing 1, a cover with a latch, and a locking pin on the outer casing 1 that matches the latch, can improve the ease of cleaning and maintenance, safety, and stability of the detection device.

[0050] Example 4 differs from the above examples in that a temperature control module is also installed inside the outer casing 1. The temperature control module includes a temperature sensor, a heater, and a cooler, all of which are connected to the controller signal.

[0051] The specific implementation process is as follows: In this embodiment, the temperature sensor can be selected from thermistors or thermocouples to measure the internal temperature of the detection device. The temperature signal can be converted into an electrical signal so that the controller can read and control the temperature of the detection device.

[0052] Heaters, such as heating elements or heating rods, can be used to increase the internal temperature of the detection device. The heater can transfer heat to the inside of the detection device through methods such as electric current or thermal radiation to achieve the required temperature.

[0053] The cooler can be a thermoelectric cooler or a compressor cooler, etc., to lower the temperature inside the detection device. The cooler can transfer heat from inside the detection device by absorbing heat or through phase change, etc., in order to achieve the required temperature.

[0054] During the immunoassay reaction, the sample and reagents need to be heated to a certain temperature to ensure the reaction proceeds. At this time, the temperature control module can raise the internal temperature of the detection device using a heater and monitor whether the temperature has reached the set range using a temperature sensor. After the immunoassay reaction is complete, the internal temperature of the detection device needs to be lowered to maintain the stability of the sample and reagents. At this time, the temperature control module can lower the internal temperature of the detection device using a cooler and monitor whether the temperature has dropped to the set range using a temperature sensor. During long-term use, it is necessary to maintain a stable internal temperature of the detection device to avoid affecting the sample and reagents. At this time, the temperature control module can alternately operate the heater and cooler to maintain a stable internal temperature within the set range.

[0055] Example 5 differs from the above examples in that: a reminder module is embedded in the outer casing 1, the reminder module includes a buzzer and an indicator light, and both the buzzer and the indicator light are connected to the controller signal.

[0056] The specific implementation process is as follows: By using a buzzer and indicator lights in combination, users can more easily determine the status and results of the detection device. For example, the buzzer can emit different sounds or frequencies to indicate different states or results, while the indicator lights can provide more intuitive prompts by displaying different colors or flashing patterns.

[0057] Once the test is complete, the buzzer will sound to notify the user that the test is finished, and the indicator light will show green or red to indicate whether the test result is positive or negative.

[0058] When the detection device malfunctions or experiences an abnormality, the buzzer can emit a specific sound or sound a continuous alarm to remind the user to take timely action or stop operation in order to avoid further damage or danger.

[0059] The above descriptions are merely embodiments of the present invention, and common knowledge such as specific structures and / or characteristics in the solutions are not described in detail here. It should be noted that those skilled in the art can make various modifications and improvements without departing from the structure of the present invention, and these should also be considered within the scope of protection of the present invention. These modifications and improvements will not affect the effectiveness of the implementation of the present invention or the practicality of the patent. The scope of protection claimed in this application should be determined by the content of its claims, and the specific embodiments described in the specification can be used to interpret the content of the claims.

Claims

1. An immunoquantitative detection device for a blood tumor marker, characterized by: The device includes an outer shell, inside which are an immune reaction module and a detection module. The immune reaction module includes a sample container, which is fixedly connected to the upper inner wall of the outer shell. The outer shell and the sample container have liquid inlets at the top. A drive unit is fixedly connected to the center of the inner top wall of the sample container. A cylindrical cam is coaxially fixedly connected to the output shaft of the drive unit. A groove is provided on the outer wall of the cylindrical cam. The groove is a vertical groove structure that runs obliquely through the groove. A slider slides inside the groove. A vertical rod is fixedly connected to the end of the slider away from the groove. A spring is sleeved on the vertical rod. A drainage tube is sleeved on the spring. The vertical rod is longer than the drainage tube. The vertical rod has a hollow structure and has round holes in the middle and bottom that communicate with the outside. The drainage tube passes through the bottom of the sample container and is fixedly connected to it. The detection module includes a detector, which is fixedly connected to the bottom wall inside the outer shell. The detector signal is connected to a controller, which is connected to a motor signal.

2. The immunoassay device for quantitative detection of blood tumor markers according to claim 1, characterized in that: One side of the casing is made of transparent hard plastic, and a display screen is fixedly connected to the casing. The display screen is connected to the controller signal.

3. The immunoassay device for quantitative detection of blood tumor markers according to claim 1, characterized in that: The detection device connects to mobile devices via wireless communication technology to enable remote monitoring and data transmission.

4. The immunoassay device for quantitative detection of blood tumor markers according to claim 1, characterized in that: The outer casing has an opening for cleaning on one side, a cover is installed at the opening, a latch is installed on the cover, and a locking pin that matches the latch is installed on the outer casing.

5. The immunoassay device for quantitative detection of blood tumor markers according to claim 1, characterized in that: The casing also houses a temperature control module, which includes a temperature sensor, a heater, and a cooler. All three components are connected to the controller via signals.

6. The immunoassay device for quantitative detection of blood tumor markers according to claim 1, characterized in that: An alert module is embedded in the outer casing. The alert module includes a buzzer and an indicator light, both of which are connected to the controller signal.

7. The immunoassay device for quantitative detection of blood tumor markers according to claim 1, characterized in that: The liquid inlet is detachably connected to a cover.

8. The immunoassay device for quantitative detection of blood tumor markers according to claim 1, characterized in that: An anti-slip layer is fixedly connected to the bottom wall of the outer shell.

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