Application of rhein as herbicide synergistic aid

Abstract

The invention relates to the field of pesticides, in particular to application of rhein as a herbicide synergistic additive. The invention discloses a herbicide synergistic aid. Rhein is used as the herbicide synergistic aid. The herbicide is a systemic transduction type broad-spectrum sterilant herbicide and is glyphosate. Rhein can be mixed with a herbicide barrel or prepared into a preparation with the herbicide for use. Rhein is used as a herbicide synergistic aid, so that the preparation cost is low; the weed death speed can be effectively increased, the herbicide dosage is reduced, and the pesticide effect is improved.

Description

Technical Field

[0001] This invention relates to the field of pesticides, specifically to the application of rhein as a herbicide synergist. Background Technology

[0002] In modern agricultural production, chemical herbicides are widely used due to their high efficiency and rapid application. However, the long-term, singular, and large-scale use of herbicides has led to numerous problems, including increased herbicide resistance, crop damage, environmental pollution, and increased costs.

[0003] Herbicides remain the most effective and cost-effective tool in weed management. To address the aforementioned issues, researchers are dedicated to developing herbicide synergists. Synergists themselves generally possess little or no herbicidal activity, but when mixed with herbicides, they can significantly enhance the activity of the herbicide, reduce its dosage, or overcome the negative effects of environmental stress. Therefore, developing highly efficient, low-toxicity, and environmentally friendly natural-source herbicide synergists is of significant practical importance.

[0004] Rhein, a natural anthraquinone compound extracted and isolated from plants such as rhubarb, a traditional Chinese medicine, can significantly enhance the activity of herbicides when applied in combination with them. In recent years, it has shown great application potential in agriculture, particularly as a herbicide synergist (or adjuvant). Its core value lies in its ability to significantly improve the efficacy of existing herbicides, thereby potentially reducing the amount of herbicides used, aligning with the development trend of "reduced application and increased efficiency" in pesticides and green agriculture. Summary of the Invention

[0005] The purpose of this invention is to provide an adjuvant that enhances the activity of herbicides and its application method, so as to solve the above-mentioned technical problems.

[0006] The technical solution of this invention is as follows:

[0007] This invention discloses the application of rhein as a herbicide synergist.

[0008] Preferably, the active ingredient of the herbicide includes glyphosate.

[0009] Preferably, the concentration ratio of rhein to glyphosate is 5-100:32.

[0010] Preferably, the herbicide targets broadleaf weeds and grass weeds. More preferably, the target weed is barnyard grass.

[0011] A novel herbicide formulation comprising the following components:

[0012] Component A: Rhein;

[0013] Component B: Glyphosate.

[0014] Preferably, the concentration ratio of rhein to glyphosate is 5-100:32.

[0015] Preferably, the target weeds of the new herbicide formulation are broadleaf weeds and grass weeds.

[0016] Preferably, the target herbicide formulation is barnyard grass.

[0017] The beneficial effects of this invention are:

[0018] This invention discloses the application of rhein in the preparation of plant herbicide synergists. The herbicide synergists containing rhein of this invention significantly improve the field control effect of herbicides on broadleaf weeds and grass weeds. They are low in cost and require less dosage, and have high application value in farmland weed control. Attached Figure Description

[0019] Figure 1 Phenotypic diagrams showing the control effects of glyphosate and different adjuvants on barnyard grass;

[0020] Figure 2 Phenotypic diagrams showing the control effects of glyphosate combined with different adjuvants on barnyard grass;

[0021] Figure 3 Phenotypic diagrams showing the control effects of different concentrations of rhein combined with recommended doses of glyphosate on barnyardgrass;

[0022] Figure 4 Phenotypic diagram showing the control effect of a mixture of rhein and glyphosate at gradient concentrations on barnyardgrass. Detailed Implementation

[0023] To make the objectives, technical solutions, and advantages of this invention clearer, the technical solutions in the embodiments of this invention will be clearly and completely described below in conjunction with the embodiments of this invention. Obviously, the described embodiments are only some embodiments of this invention, not all embodiments. Based on the embodiments of this invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this invention.

[0024] Example 1: Control effect of glyphosate with different adjuvants (single agent) and field recommended dosage on barnyard grass.

[0025] Barnyard grass was cultivated in a pot experiment under artificial climate chamber conditions. The effects of recommended doses of glyphosate and different adjuvants (resveratrol (Res), baicalin (Bai), curcumin (Cur), camptothecin (Cam), quercetin (Que), rhein (Rhe), simvastatin (Sim), betaine (Bet), and fluorescein (Flu)) on the bioactivity of barnyard grass were investigated using the following methods:

[0026] 1. Sow barnyard grass seeds in plastic pots with a top diameter of 9 cm, cover with 0.5 cm of soil (enough to cover the weed seeds), then place the pots in enamel dishes filled with water and water using bottom irrigation. Transfer the pots to an artificial climate chamber for cultivation. The soil used in the experiment consisted of top 20 cm loam and culture medium mixed at a ratio of 3:1.

[0027] 2. In an artificial climate chamber (16 h light exposure at 100-120 μmol / m²), -2 .s -1 Weeds were cultivated at 22 / 18°C.

[0028] 3. Indoors, use the ASS-4 type automatic control spraying system for pesticide spraying, with fan-shaped nozzles. Combine nozzle pressure and flow rate, etc., according to the actual spraying area (1.1 m²). 2 Spray 50 mL of pesticide solution (equivalent to 30 L of water per acre), adjust the operating speed, and evenly arrange the plastic basins to be treated on the spraying platform for uniform spraying. Spray pressure: 0.35 MPa; fan nozzle flow rate: 800 mL / min. Spray from low to high dosage sequentially. Repeat each treatment 3 times. After spraying, allow to dry for one day, then place in an artificial climate chamber and water regularly to keep the soil moist.

[0029] 4. The blank control was an administration of an ethanol-Tween 80 aqueous solution without any added drug;

[0030] 5. The concentration of glyphosate solution used is 3.2 mg / mL. -1 The concentration of each adjuvant in the drug solution is 10 mg / mL. -1 ;

[0031] 6. Seven days after application of the pesticide, take photos to record the symptoms of weed damage. The results are as follows: Figure 1 The above-ground fresh weight of the weeds in each treatment was measured 7 days later, and the fresh weight control efficacy (%) of each treatment was calculated using the following formula:

[0032] E = 100 × (CT) / C

[0033] In the formula, E represents the fresh weight efficacy; C represents the fresh weight of the control aboveground parts; and T represents the fresh weight of the treated aboveground parts.

[0034] Table 1. Control efficacy of glyphosate against barnyard grass by single application of different adjuvants and field recommended dosage.

[0035]

[0036] Table 1 and Figure 1 The results showed that the individual adjuvants resveratrol, baicalein, curcumin, camptothecin, quercetin, rhein, simvastatin, betaine, and fluorescein did not possess herbicidal activity.

[0037] Example 2: Control effect of different adjuvants combined with glyphosate field recommended dosage on barnyard grass

[0038] The planting conditions and application methods for barnyard grass were the same as in Example 1. The bioactivity of glyphosate combined with different adjuvants on barnyard grass was studied using foliar treatments. The specific methods are as follows:

[0039] 1. The blank control group was treated with ethanol-Tween 80 aqueous solution without any glyphosate, while the treatment groups were treated with glyphosate and glyphosate with different agonists.

[0040] 2. The concentration of glyphosate solution used is 3.2 mg / mL. -1 The concentration of different adjuvants in the compound drug solution is 10 mg / mL. -1 ;

[0041] 3. Seven days after application of the pesticide, take photos to record the symptoms of weed damage. The results are as follows: Figure 1 The above-ground fresh weight of the weeds in each treatment was measured 7 days later, and the fresh weight control efficacy (%) of each treatment was calculated using the following formula:

[0042] E = 100 × (CT) / C

[0043] In the formula, E represents the fresh weight efficacy; C represents the fresh weight of the control aboveground parts; and T represents the fresh weight of the treated aboveground parts.

[0044] Table 2. Control effects of different adjuvants combined with glyphosate field recommended dosage on barnyardgrass.

[0045]

[0046] Table 2 and Figure 2 The results showed that the recommended field dose of glyphosate was similar to that of rhein at 10 mg / mL. -1 Seven days after the compound formulation was applied, the fresh weight inhibition rate of barnyard grass reached 90.2%. Therefore, adding rhein as an adjuvant to glyphosate can increase its fresh weight inhibition rate and accelerate its lethality against barnyard grass.

[0047] Example 3: Control effect of different concentrations of rhein combined with the field-recommended dose of glyphosate on barnyard grass.

[0048] The planting conditions and application methods for barnyard grass were the same as in Example 1. The bioactivity of foliar treatment with different concentrations of rhein and the recommended field dose of glyphosate on barnyard grass was studied. The specific methods are as follows:

[0049] 1. The blank control was the application of ethanol-Tween 80 aqueous solution without any drug, the positive control was glyphosate alone, and the treatment group was glyphosate with added rhein;

[0050] 2. The concentration of glyphosate solution used is 3.2 mg / mL. -1 The concentration of rhein in the drug solution was 0.05 mg / mL. -1 0.1 mg mL -1 0.5 mg mL -1 1mg mL -1 2.5 mg mL -1 5 mg mL -1 10 mg mL -1 25 mg / mL -1 ;

[0051] 3. Seven days after application of the pesticide, take photos to record the symptoms of weed damage. The results are as follows: Figure 3 The above-ground fresh weight of the weeds in each treatment was measured 7 days later, and the fresh weight control efficacy (%) of each treatment was calculated using the following formula:

[0052] E = 100 × (CT) / C

[0053] In the formula, E represents the fresh weight efficacy; C represents the fresh weight of the control aboveground parts; and T represents the fresh weight of the treated aboveground parts.

[0054] The experimental results are shown in Table 3.

[0055] Table 3. Control effects of different concentrations of rhein combined with the recommended field dose of glyphosate on barnyard grass.

[0056]

[0057] Table 3 and Figure 3 The results showed that glyphosate at the recommended field dose inhibited the fresh weight of barnyardgrass by only 56.6% seven days after application. The recommended field dose of glyphosate was comparable to that of rhein (1 mg / mL). -1 Seven days after application, the fresh weight inhibition rate of rhein in barnyard grass reached 82.7%. Therefore, the optimal recommended concentration for compound application of rhein is 1 mg / mL. -1In summary, adding different concentrations of rhein as an adjuvant to glyphosate can increase its fresh weight inhibition rate and accelerate its lethality against barnyard grass.

[0058] Example 4: The optimal recommended combination concentration of rhein with different glyphosate concentrations for barnyard grass control.

[0059] The planting conditions and application methods for barnyard grass were the same as in Example 1. The bioactivity of stem and leaf treatments with different concentrations of glyphosate mixed with the optimal recommended concentration of rhein on barnyard grass was studied. The specific methods are as follows:

[0060] 1. The blank control was an ethanol-Tween 80 aqueous solution without pesticides, the positive control was the optimal recommended concentration of rhein and the field recommended concentration of glyphosate alone, and the treatment group was glyphosate with added rhein;

[0061] 2. The concentration of rhein solution used is: 1 mg / mL -1 The glyphosate application rate is 0.4 mg / mL. -1 0.8 mg mL -1 1.6 mg mL -1 3.2 mg mL -1 6.4 mg mL -1 12.8 mg mL -1 ;

[0062] 3. Seven days after application of the pesticide, photographs were taken to record the symptoms of barnyard grass damage. The results were as follows: Figure 4 The above-ground fresh weight of the weeds in each treatment was measured 7 days later, and the fresh weight inhibition rate was calculated.

[0063] Fresh weight inhibition rate = (average fresh weight of control - average fresh weight of treatment) / average fresh weight of control × 100%.

[0064] Table 4. Control effects of different concentrations of glyphosate combined with the optimal recommended concentration of rhein on barnyard grass.

[0065]

[0066] Table 4 and Figure 4 The results showed that the optimal recommended concentration of rhein in the compound formulation was 1 mg / mL. -1 The study compared the fresh weight inhibition rate of barnyardgrass with different concentrations of glyphosate, showing that the optimal recommended concentration of rhein in the compound formulation was 1 mg / mL. -1 With glyphosate 6.4 mg mL -1 The combination of herbs with barnyard grass has the best control effect.

[0067] Example 5: Field control effect of rhein combined with glyphosate on barnyard grass.

[0068] Select typical non-cultivated weed-prone areas to evaluate the field efficacy of the optimal indoor ratio of glyphosate and rhein against barnyardgrass. The specific methods are as follows:

[0069] 1. The experimental site was selected at the Gaoqiao Demonstration Base of the Hunan Academy of Agricultural Sciences (27.9565°N, 114.1283°E), with each plot having an area of ​​2 m². 2 Evenly scatter barnyard grass seeds, and treat them when they grow to the 3-4 leaf stage. Repeat the experiment three times.

[0070] 2. The experiment consisted of four treatments: the optimal ratio of rhein to glyphosate as shown in Example 4, rhein alone, glyphosate alone, and a blank control treated with an ethanol-Tween 80 aqueous solution without any pesticides. The experiment was designed with three replicates, totaling 12 plots arranged in a randomized block design. Each plot was 1 m × 2 m in size, planted in 4 rows and 3 columns. Irrigation and other management practices followed local farming customs.

[0071] 3. The single-dose concentration of rhein is 1 mg / mL. -1 The glyphosate application rate was 6.4 mg / mL. -1 The combined application concentration of rhein and glyphosate is 1 + 6.4 mg / mL. -1 ;

[0072] 5. Seven days after application, photographs were taken to record the symptoms of weed damage. The results are shown in Table 5. Seven days later, the fresh weight of the above-ground portion of the weeds in each treatment was weighed, and the fresh weight inhibition rate was calculated.

[0073] Fresh weight inhibition rate = (average fresh weight of control - average fresh weight of treatment) / average fresh weight of control × 100%.

[0074] Table 5. Field control effects of rhein and glyphosate on barnyard grass.

[0075]

[0076] Table 5 shows that the optimal ratio of rhein to glyphosate significantly improved barnyardgrass control 7 days after application compared to single-agent application.

Claims

1. Application of rhein as a herbicide synergist.

2. The application according to claim 1, characterized in that, The active ingredient in the herbicide includes glyphosate.

3. The application according to claim 2, characterized in that, The concentration ratio of rhein to glyphosate is 5-100:

32.

4. The application according to claim 3, characterized in that, The concentration ratio of rhein to glyphosate is 5:

32.

5. The application according to any one of claims 1-4, characterized in that, The herbicide targets barnyard grass.

6. A novel herbicide formulation, characterized in that, The novel herbicide formulation comprises the following components: Component A: Rhein; Component B: Glyphosate.

7. The novel herbicide formulation according to claim 6, characterized in that, The concentration ratio of rhein to glyphosate is 5-100:

32.

8. The novel herbicide formulation according to claim 7, characterized in that, The concentration ratio of rhein to glyphosate is 5:

32.

9. The novel herbicide formulation according to any one of claims 6-8, characterized in that, The target herbicide formulation is barnyard grass.

Images