Synthetic bacterial community of ligusticum chuanxiong hance endophytic bacteria, and use and plant protection agent for preventing and treating root rot and method for cultivating disease-resistant seedlings

Abstract

The present application belongs to the field of biological control, and particularly relates to a synthetic bacterial community of Ligusticum chuanxiong Hara endophytic bacteria, uses thereof, a plant protection agent for preventing and treating root rot, and a method for cultivating disease-resistant seedlings. Plantibacter flavus (P36-15), Microbacterium schleiferi (P48-28), Rothia aeria (P48-75), are proportionally compounded, inoculated into Ligusticum chuanxiong Hara seedlings, can prevent root rot caused by Fusarium oxysporum and Fusarium solani, improve the ability of Ligusticum chuanxiong Hara plants to resist root rot, improve the field control effect and medicinal material yield for preventing and controlling Ligusticum chuanxiong Hara Fusarium oxysporum and Fusarium solani type root rot, and have popularization and application value.

Description

Technical Field

[0001] This invention belongs to the field of biological control, specifically relating to a synthetic bacterial community of endophytic bacteria of Ligusticum chuanxiong and its uses, a plant protectant for controlling root rot, and a method for cultivating disease-resistant seedlings. Background Technology

[0002] Chuanxiong is a plant of the Apiaceae family called Ligusticum chuanxiong. Oak chuanxiong The dried rhizome of Hort. is a commonly used traditional Chinese medicine for promoting blood circulation and regulating qi. It is pungent and warm in nature and enters the liver, gallbladder and pericardium meridians. It has the effects of promoting blood circulation and removing blood stasis, regulating qi and relieving pain, and dispelling wind and dispersing. Clinically, it is often used for dysmenorrhea, chest pain, headache and rheumatic pain caused by blood stasis and qi stagnation. Modern research has also confirmed that it can improve microcirculation and inhibit platelet aggregation. It is a representative of "blood qi medicine".

[0003] Ligusticum chuanxiong, mainly produced in Sichuan Province, is a traditional Chinese medicine. However, its cultivation has long been plagued by root rot caused by Fusarium oxysporum and Fusarium solani. These pathogens infect the roots, causing rot and seedling death, reducing the efficacy of medicinal herbs, and hindering the sustainable development of the industry. Current control methods include agricultural control, which is limited by farming conditions, lacks specificity in inhibiting bacteria, and has little effect on already diseased plants; chemical control is prone to leaving pollution. Therefore, biological control strategies that utilize artificially introduced highly efficient biocontrol bacteria and reconstruct the plant's microecology to achieve green balance and promote host health are becoming a research hotspot and sustainable alternative for the control of root rot in Ligusticum chuanxiong. However, in practical applications, due to poor environmental adaptability and low colonization efficiency, exogenous bacteria often fail to achieve ideal control effects. Therefore, constructing synthetic microbial communities with long-term stable colonization capabilities is considered the core breakthrough. Summary of the Invention

[0004] To address the unsatisfactory practical application effect of biological control of root rot in Ligusticum chuanxiong, this invention provides a synthetic bacterial community of endophytic bacteria in Ligusticum chuanxiong, which is composed of any combination of Microbacterium schrenckii, L. airborne bacteria, and Bacillus flavus.

[0005] The *Schönleinii* species is *Schönleinii*. Microbacterium schleiferi P48-28, deposited in the Medicinal Plant Microbiology Bank of the National Germplasm Resource Bank of Chinese Medicinal Herbs, accession number: LiCh24RhB051;

[0006] The *Rochetomyces airborne* is *Rochetomyces airborne*. Rothia aerea P48-75, deposited in the Medicinal Plant Microbiology Bank of the National Germplasm Resource Bank of Chinese Medicinal Herbs, accession number: LiCh24RhB054;

[0007] The yellow plant bacteria is yellow plant bacteria. Plantibacter flavus P36-15 is deposited in the Medicinal Plant Microbial Bank of the National Germplasm Resource Bank of Chinese Medicinal Herbs, accession number: LiCh24RhB030.

[0008] Furthermore, it is composed of Microbacterium schlegelii, L. airborne bacteria, and Bacillus flavus;

[0009] The CFU ratio of *Microbacterium schlegelii*, *Rochetomyces airborneum*, and *Flavobacterium chrysogenum* is 1-5:1-5:1-5.

[0010] The present invention also provides the use of the aforementioned synthetic bacterial community in the cultivation of disease-resistant seedlings.

[0011] Furthermore, the disease-resistant seedlings are Ligusticum chuanxiong seedlings resistant to root rot;

[0012] The root rot mentioned is a root rot caused by fungi of the genus Fusarium;

[0013] The Fusarium fungi mentioned include Fusarium oxysporum and Fusarium solanum.

[0014] The present invention also provides the use of the aforementioned synthetic bacterial community in the preparation of plant protectants for the prevention and / or treatment of root rot.

[0015] Furthermore, the plant protectant has the effect of inhibiting the pathogens causing root rot in Ligusticum chuanxiong.

[0016] Furthermore, the pathogenic bacteria include fungi of the genus Fusarium; the fungi of the genus Fusarium include Fusarium oxysporum and Fusarium solani.

[0017] The present invention also provides a plant protectant for preventing root rot, which is a formulation made with the aforementioned synthetic bacterial community as the active ingredient and acceptable excipients.

[0018] The formulations include solutions, granules, powders, ointments, and sprays;

[0019] The solution includes microbial fertilizer aqueous solution, the granules include microbial fertilizer granules, the powder includes microbial fertilizer powder, and the paste includes microbial fertilizer paste.

[0020] Furthermore, the viable count of the synthetic bacterial community in the formulation is ≥1×10⁻⁶. 7 CFU is characterized by inoculating the aforementioned endogenous synthetic bacterial community into plant seedlings;

[0021] The plant seedlings mentioned are Ligusticum chuanxiong seedlings;

[0022] The Ligusticum chuanxiong seedlings include aseptic tissue culture seedlings of Ligusticum chuanxiong;

[0023] Each Ligusticum chuanxiong seedling was inoculated with a synthetic bacterial community of 1~10×10⁶. 7 CFU.

[0024] The "synthetic bacterial community" described in this invention is an artificial microbial system that can perform a predetermined function and is stable and controllable, constructed by artificially combining microbial strains with clearly defined classifications and functions in a certain proportion.

[0025] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0026] The present invention synthesizes a bacterial community of endophytic bacteria from Ligusticum chuanxiong, by using three endophytic bacteria strains screened from Ligusticum chuanxiong. P.flavus (P36-15) M.schleiferi (P48-28) R.aeria (P48-75), when mixed in proportion and inoculated at the roots of Ligusticum chuanxiong seedlings, can significantly inhibit the root rot pathogens Fusarium oxysporum and Fusarium solanum, enhance the resistance of Ligusticum chuanxiong plants to root rot, improve the field control efficacy and yield of medicinal materials for controlling Fusarium oxysporum and Fusarium solanum root rot, and has the value for promotion and application.

[0027] Obviously, based on the above description of the present invention, and according to common technical knowledge and conventional methods in the field, various other modifications, substitutions or alterations can be made without departing from the basic technical concept of the present invention.

[0028] The following detailed embodiments further illustrate the above-described content of the present invention. However, this should not be construed as limiting the scope of the present invention to the following examples. All technologies implemented based on the above-described content of the present invention fall within the scope of the present invention. Attached Figure Description

[0029] Figure 1 Neighbor-joining phylogenetic tree constructed based on rDNA-16S base sequence;

[0030] Figure 2 Bacterial screening using the streak plate method;

[0031] Figure 3 In vitro pathogenicity of the strain inoculated with Ligusticum chuanxiong leaves;

[0032] Figure 4 In vivo pathogenicity study of strains inoculated with Ligusticum chuanxiong tissue culture seedlings (blank represents uninoculated strains, pathogens are...) Fusarium solani Fusarium solani S3-6 and Fusarium oxysporum Fusarium oxysporum S-4).

[0033] Figure 5 Growth and disease incidence of Ligusticum chuanxiong seedlings under synthetic microbial community treatment (the numbers in the lower right corner of SCP, SCT, and FUS represent the disease severity grading of Ligusticum chuanxiong root rot). Detailed Implementation

[0034] The raw materials, reagents, and equipment used in the specific embodiments of this invention were all purchased commercially.

[0035] Example 1: Construction and efficacy experiment of endophytic synthetic bacterial community of Ligusticum chuanxiong.

[0036] Three endophytic bacteria were isolated from the rhizome of Ligusticum chuanxiong: Plantibacter flavus (P36-15) Microbacterium schleiferi (P48-28) Rothia aerea (P48-75). A Neighbor-joining phylogenetic tree constructed based on the rDNA-16S base sequence for strains P36-15, P48-28, and P48-75 is shown below. Figure 1 .

[0037] from Figure 1 It is evident that strain P48-28 exists in an independent environment, distinct from known strains. Microbacterium schleiferi The clearly separated branches, with high support, indicate that it is a novel strain of *Microbacterium stearotherum*. Microbacterium schleiferi This strain was classified and named: Microbacterium schrenckii. Microbacterium grinders It is currently deposited in the Medicinal Plant Microbial Bank of the National Germplasm Bank of Chinese Medicinal Herbs https: / / zyd.cdutcm.edu.cn / zyzzzy, accession number: LiCh24RhB051, deposit address: Chengdu University of Chinese Medicine, No. 1166 Liutai Avenue, Wenjiang District, Chengdu, Sichuan Province;

[0038] strain P48-75 was in an independent, known... Rothia aerea The clearly separated branches of the sequence, and the high support of these branches, indicate that it is a novel strain of *Rhodotorula avium*. Rothia aerea This strain was classified and named: *Rochetomyces airborne*. Rothia aerea It is currently deposited in the Medicinal Plant Microbial Bank of the National Germplasm Bank of Chinese Medicinal Herbs https: / / zyd.cdutcm.edu.cn / zyzzzy, accession number: LiCh24RhB054, deposit address: Chengdu University of Chinese Medicine, No. 1166 Liutai Avenue, Wenjiang District, Chengdu, Sichuan Province;

[0039] strain P36-15 was in an independent, known... Plantibacter flavus The clearly separated branches of the sequence, and the high support of these branches, indicate that it is a new strain of *Bacillus flavovirens*. Plantibacter flavus This strain was classified and named: *Flavobacterium flavonoids*. Plantibacter flavusIt is currently deposited in the Medicinal Plant Microbial Bank of the National Germplasm Resource Bank of Chinese Medicinal Herbs https: / / zyd.cdutcm.edu.cn / zyzzzy, accession number: LiCh24RhB030, deposit address: Chengdu University of Chinese Medicine, No. 1166 Liutai Avenue, Wenjiang District, Chengdu, Sichuan Province;

[0040] The strain information of the above three strains was officially published on February 10, 2026, through the "Information Disclosure of Strains of the Innovative Research Platform for Microecology of Traditional Chinese Medicine" column on the WeChat official account "Weishuo Bencao". The public can obtain it through the National Germplasm Resource Bank of Traditional Chinese Medicine of Chengdu University of Traditional Chinese Medicine or through the applicant.

[0041] 2. Construction of synthetic microbial communities

[0042] 2.1 Compatibility assessment of strains (pairwise streak method)

[0043] Prepare LB solid culture plates and place three candidate strains on them. P. flavus (P36-15) M. schleiferi (P48-28) R. aeria (P48-75) Inoculate the plating lines in pairs onto LB agar plates and incubate at 37°C for 24 hours. Observe whether a blank inhibition band appears at the intersection of the streaks (no inhibition band indicates no antagonism and synergistic growth). Results are shown below. Figure 2 .

[0044] The results of the pairwise underlining show that these three endophytic fungi of Ligusticum chuanxiong P. flavus (P36-15) M. schleiferi (P48-28) R. aeria (P48-75) can grow synergistically.

[0045] 2.2 Pathogenicity assessment of the strain

[0046] (1) In vitro experiments (inoculation of detached leaflets)

[0047] Experimental Design: Two strains of pathogenic fungi (Fusarium oxysporum) isolated from *Ligusticum chuanxiong* suffering from root rot in the laboratory were preserved in the Medicinal Plant Microbial Bank of the National Germplasm Bank of Chinese Medicinal Herbs (Chengdu University of Chinese Medicine). Fusarium oxysporum (S-4), Fusarium solani Fusarium solani (S 3-6) served as two disease control groups, uninoculated PDB and brain heart infusion (BHI) culture media served as two blank control groups, and three bacterial cell blocks (P36-15, P48-28, P48-75) served as three experimental groups.

[0048] Specific operating procedures: Mature leaves of healthy Ligusticum chuanxiong were cut and sterilized with ultraviolet light for 30 minutes in a clean bench. The leaves were then placed flat on a petri dish lined with filter paper using sterile forceps. The leaf surface was gently incised with a sterile blade to create wounds. Pathogenic fungi were extracted using a 5 mm punch and inoculated onto the wound surface. Bacteria were inoculated onto the leaf surface using a bacterial suspension spot method. Filter paper and cotton soaked in sterile water were used to provide the leaves with the necessary moisture. The leaves were cultured for 5 days, and the lesion status was observed to determine the potential pathogenicity of the strain to Ligusticum chuanxiong.

[0049] See results Figure 3 The in vitro pathogenicity of these three endophytic fungi from *Ligusticum chuanxiong* was examined by inoculating detached leaves. P. flavus (P36-15) M. schleiferi (P48-28) R. aeria (P48-75) It has no potential pathogenicity to the host, Ligusticum chuanxiong.

[0050] (2) In vivo experiments (inoculation of tissue culture seedlings)

[0051] The potential pathogenicity of each bacterial strain to *Ligusticum chuanxiong* plants was further investigated using a tissue culture seedling inoculation method. A mixed pathogenic bacteria treatment served as a disease control group, an uninoculated treatment served as a blank control group, and three bacterial strains were used as three experimental groups. The culture apparatus consisted of tissue culture bottles, and the culture medium was rooting medium. One month after rooting, five bottles were used per group, with one rooted *Ligusticum chuanxiong* seedling per bottle. The roots were inoculated with the pathogenic bacteria / bacteria. The bacterial concentration was 10... 5 CFU / mL, 20 μL per bottle, cultured for 14 days, and observed the disease incidence of rooted Ligusticum chuanxiong seedlings.

[0052] See results Figure 4 The tissue culture seedlings were inoculated and tested for pathogenicity in vivo, and no pathogenicity was found.

[0053] Inter-strain compatibility ( Figure 2 ) and host pathogenicity ( Figure 3~4 The testing experiments confirmed that these three endophytic fungi of Ligusticum chuanxiong, which have good compatibility and are disease-free on Ligusticum chuanxiong plants, can be used. P. flavus (P36-15) M. schleiferi (P48-28) R. air (P48-75), used to construct synthetic communities.

[0054] 3. Effect of synthetic microbial communities on the control of root rot in Ligusticum chuanxiong and cultivation of disease-resistant seedlings

[0055] 3.1 Effect of Synthetic Microbial Community on Controlling Root Rot of Ligusticum chuanxiong

[0056] Healthy and similarly vigorous Ligusticum chuanxiong tissue culture seedlings were selected for inoculation treatment. Experimental groups included: control (CK), synthetic bacterial culture (SC), synthetic bacterial culture followed by pathogenic bacteria (SCP), pathogenic bacteria followed by synthetic bacterial culture (SCT), and pathogenic bacteria (FUS). Each group consisted of 12 pots, with 2 seedlings per pot, for a total of 5 groups.

[0057] (1) CK plants were not inoculated and were irrigated with 2 mL of sterile water per plant;

[0058] (2) The SC group was inoculated with only 2 mL / strain of synthetic bacterial culture;

[0059] (3) The SCP group was first inoculated with 2 mL of synthetic bacterial culture per plant, and 5 days later, it was inoculated with 2 mL of mixed pathogenic bacterial culture per plant.

[0060] (4) The SCT group was first inoculated with 2 mL of mixed pathogenic bacterial solution per plant, and 5 days later, 2 mL of synthetic bacterial solution per plant was inoculated.

[0061] (5) The disease group (FUS group) was inoculated with only 2 mL of mixed pathogenic bacterial solution per plant;

[0062] Synthetic community bacterial suspension (10 7 (CFU / mL) represents the concentrations of the three bacterial strains, which are 10... 7 CFU / mL were mixed in equal proportions (1:1:1); the mixed pathogenic bacterial suspension (10 5 (CFU / mL) represents the concentrations of the two pathogens, each at 10... 5 CFU / mL, mixed in an equal ratio (1:1).

[0063] Weigh and record the initial weight, and culture continuously for 20 days. During this period, weigh and replenish water every 5 days, and water with modified Hoagland nutrient solution once every 10 days.

[0064] After 20 days of co-culturing with the microorganisms, the *Ligusticum chuanxiong* seedlings were gently removed from the pots with tweezers. The roots were carefully washed with sterile water, and excess moisture was wiped dry with sterile filter paper. Based on the disease severity grading criteria for *Ligusticum chuanxiong* root rot (Table 1), the disease index of the CK, SC, SCP, SCT, and FUS groups was calculated, as well as the control efficacy of the SCP group. The control efficacy refers to the effect of the synthetic microbial community on the control of *Ligusticum chuanxiong* root rot caused by the pathogen. The calculation formula is as follows:

[0065] Table 1 Grading Criteria for Root Rot Disease in Ligusticum chuanxiong

[0066]

[0067] Disease index (DI) = [∑(total number of diseased plants at each level × representative value) / (total number of plants surveyed × representative value of the highest disease level)] × 100%;

[0068] Control efficiency (CE) = (DI of disease group - DI of treatment group) / DI of disease group × 100%.

[0069] The disease prevention effects of each group are shown in Table 2 and Figure 5 .

[0070] Table 2. Disease resistance effect of synthetic microbial communities on Ligusticum chuanxiong seedlings

[0071]

[0072] Note: Different lowercase letters in the same column indicate significant differences between treatment groups. P <0.05)

[0073] As can be seen from the above, administering synthetic microbial communities to Ligusticum chuanxiong seedlings (SCP group) can enhance the plant's resistance to pathogens. Compared with the pathogen-infected FUS group, the disease index was reduced by 52%, and the control efficacy was as high as 74.29%. Administering synthetic microbial communities to diseased Ligusticum chuanxiong seedlings (SCT group) also showed a certain therapeutic effect. Prioritizing inoculation with synthetic microbial communities can reduce the severity of root rot in Ligusticum chuanxiong, achieving a disease "prevention" effect.

[0074] In the preliminary experiments, the inhibitory effect of any single strain of the synthetic microbial community on pathogens was not as good as that of the synthetic microbial community. In the formal pot experiment, regardless of whether the synthetic microbial community was inoculated first or later, the plants were significantly resistant to root rot, indicating that the synthetic community can quickly occupy the ecological niche in the rhizosphere and continuously suppress the disease. Its robust colonization and competitiveness have met the requirements for cultivating resistant seedlings of Ligusticum chuanxiong.

[0075] 3.2 Cultivation of disease-resistant seedlings of Ligusticum chuanxiong

[0076] Synthetic communities have demonstrated strong community stability and disease resistance in simulated rhizosphere environments, providing strong evidence for their colonization and disease resistance in complex field environments. Based on the cultivation methods of Ligusticum chuanxiong seedlings, a method for large-scale cultivation of disease-resistant Ligusticum chuanxiong seedlings is presented below:

[0077] Step 1: Cultivation of Aseptic Tissue Culture Seedlings of Ligusticum chuanxiong: Tender stem and node tissues of Ligusticum chuanxiong are collected and inducing callus, shoot clustering, and rooting to become aseptic tissue culture seedlings of Ligusticum chuanxiong, providing plant material for subsequent cultivation of disease-resistant seedlings. The callus culture conditions are MS + sucrose + agar + 0.6~1 mg / L 6-BA + 2.5~3 mg / L NAA; the shoot clustering culture conditions are MS + sucrose + agar + 0.5~1 mg / L 6-BA + 0.3~0.8 mg / L IAA; and the rooting culture conditions are MS + sucrose + agar + 0.5~1 mg / L IAA.

[0078] Step 2: Preparation of bacterial culture solution for synthesizing bacterial flora: 10 μL of bacterial culture solution containing 3 bacterial strains. 7 CFU / mL, mixed in equal proportions, to prepare a synthetic bacterial solution, to be used immediately after preparation.

[0079] Step 3: Acclimation of Tissue Culture Seedlings: In a clean bench, transfer the *Ligusticum chuanxiong* tissue culture seedlings from the rooting medium to sterile potting containers filled with shale and vermiculite for acclimation. At transplanting, water each seedling with 5 mL of modified Hoagland nutrient solution and 100 mL of sterile water, record the initial weight, and cover with a transparent sterile plastic film. Culture for 7 days under the following conditions: 18–25 ℃, 7–14 h light, 60–80% humidity, and 4000–6000 Lux light intensity. During the culture period, weigh and water the seedlings according to their growth requirements.

[0080] Step Four: Cultivation of Disease-Resistant Seedlings: After a week of acclimatization, select robust potted seedlings for cultivation as disease-resistant seedlings. Inoculate each seedling with 5 mL of a 10% concentration at the root. 7 A CFU / mL synthetic bacterial culture was prepared, and an appropriate amount of sterile water was added to bring it to the initial weight. The culture was then covered with a transparent sterile plastic film. The culture was continuously incubated for 20 days, with water replenished every 5 days during this period.

[0081] Step 5: Hardening off and transplanting disease-resistant seedlings: Remove the plastic wrap from the potted disease-resistant seedlings and continue to cultivate them for 7 days to allow them to adapt to the external environment.

[0082] Gently remove the resistant seedlings from the pots, leaving some of the substrate soil around the roots, and transplant them to the field.

[0083] In summary, this invention utilizes three endophytic bacteria screened from Ligusticum chuanxiong. P.flavus (P36-15) M.schleiferi (P48-28) R.aeria (P48-75), when mixed in proportion and inoculated at the roots of Ligusticum chuanxiong seedlings, can significantly inhibit the root rot pathogens Fusarium oxysporum and Fusarium solanum, enhance the resistance of Ligusticum chuanxiong plants to root rot, improve the field control efficacy and yield of medicinal materials for controlling Fusarium oxysporum and Fusarium solanum root rot, and has the value for promotion and application.

Claims

1. A synthetic bacterial community of endophytic bacteria from Ligusticum chuanxiong, characterized in that: It is composed of Microbacterium schlegelii, Lorva airborne bacteria, and Bacillus flavus; The *Schönleinii* species is *Schönleinii*. Microbacterium schleiferi P48-28, deposited in the Medicinal Plant Microbiology Bank of the National Germplasm Resource Bank of Chinese Medicinal Herbs, accession number: LiCh24RhB051; The *Rochetomyces airborne* is *Rochetomyces airborne*. Rothia aeria P48-75, deposited in the Medicinal Plant Microbiology Bank of the National Germplasm Resource Bank of Chinese Medicinal Herbs, accession number: LiCh24RhB054; The yellow plant bacteria is yellow plant bacteria. Plantibacter flavus P36-15 is deposited in the Medicinal Plant Microbial Bank of the National Germplasm Resource Bank of Chinese Medicinal Herbs, accession number: LiCh24RhB030.

2. The synthetic bacterial community according to claim 1, characterized in that: The CFU ratio of *Microbacterium schlegelii*, *Rochetomyces airborneum*, and *Flavobacterium chrysogenum* is 1-5:1-5:1-5.

3. The use of the synthetic bacterial community according to claim 1 or 2 in the cultivation of disease-resistant seedlings, characterized in that: The disease-resistant seedlings are Ligusticum chuanxiong seedlings resistant to root rot.

4. The use according to claim 3, characterized in that: The root rot is a root rot caused by fungi of the genus Fusarium; the fungi of the genus Fusarium include Fusarium oxysporum and Fusarium solanum.

5. The use of the synthetic bacterial community of claim 1 in the preparation of plant protectants for the prevention and / or treatment of root rot, characterized in that: The plant protectant has the effect of inhibiting the pathogens causing root rot in Ligusticum chuanxiong.

6. The use as described in claim 5, characterized in that: The pathogenic bacteria include fungi of the genus Fusarium; the fungi of the genus Fusarium include Fusarium oxysporum and Fusarium solani.

7. A plant protectant for preventing root rot, characterized in that: It is a formulation made with the synthetic bacterial community as described in claim 1 or 2 as the active ingredient, plus acceptable excipients; The formulations include solutions, granules, powders, ointments, and sprays; The solution includes microbial fertilizer aqueous solution, the granules include microbial fertilizer granules, the powder includes microbial fertilizer powder, and the paste includes microbial fertilizer paste.

8. The plant protectant as described in claim 7, characterized in that: The viable count of the synthetic bacterial community in the formulation is ≥1×10⁻⁶. 7 CFU / mL.

9. A method for cultivating disease-resistant seedlings, characterized in that: It includes inoculating the synthetic bacterial community of the endophytic bacteria of Ligusticum chuanxiong as described in claim 1 or 2 into the plant seedlings; The plant seedlings mentioned are Ligusticum chuanxiong seedlings; The Ligusticum chuanxiong seedlings include aseptic tissue culture seedlings of Ligusticum chuanxiong; Each Ligusticum chuanxiong seedling was inoculated with a synthetic bacterial community of 1~10×10⁶. 7 CFU.

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